Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1230790 | Msa1273410 | 0.832365 | 9.854422e-56 | -8.615850e-47 |
| Msa1273410 | Msa1347270 | 0.822315 | 2.529528e-53 | -8.615850e-47 |
| Msa1273410 | Msa1378160 | 0.899738 | 1.471676e-77 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCTCCTTTCACAAAGTTT+TGG | 0.209669 | 8_3:+7143787 | None:intergenic |
| ATATCATATAAAGGTGGAAA+TGG | 0.362622 | 8_3:+7143611 | None:intergenic |
| GCTTGTCATCGTCAAGGCTT+TGG | 0.378692 | 8_3:-7144048 | Msa1273410:CDS |
| AATGTGGAGCATGCTACTTT+TGG | 0.451256 | 8_3:-7144072 | Msa1273410:CDS |
| AAGCAAAACCAATGCCTTGA+CGG | 0.461808 | 8_3:+7143677 | None:intergenic |
| GCTTGTCACCGTCAAGGCAT+TGG | 0.463579 | 8_3:-7143685 | Msa1273410:CDS |
| AGGAGAAGCAAAACATGGTC+AGG | 0.478213 | 8_3:-7143772 | Msa1273410:CDS |
| AAACGAAGTAAAACATGGTC+AGG | 0.485517 | 8_3:-7144135 | Msa1273410:CDS |
| AATGTGGAGCATGCAACTTC+TGG | 0.503666 | 8_3:-7143709 | Msa1273410:CDS |
| TCAGGACCTTGTCTTATCTC+AGG | 0.512067 | 8_3:-7143754 | Msa1273410:CDS |
| CAGTTTCCTGTGTTAAGACA+AGG | 0.529102 | 8_3:+7144111 | None:intergenic |
| TTTGGTGCTTGTCATCGTCA+AGG | 0.545774 | 8_3:-7144054 | Msa1273410:CDS |
| TAAAAGACAATGCGTTAATG+TGG | 0.555166 | 8_3:-7144088 | Msa1273410:CDS |
| CAATTTCCTGAGATAAGACA+AGG | 0.570373 | 8_3:+7143748 | None:intergenic |
| TATGAAATATCATATAAAGG+TGG | 0.585944 | 8_3:+7143605 | None:intergenic |
| TGGAACCAAAACTTTGTGAA+AGG | 0.587476 | 8_3:-7143792 | Msa1273410:CDS |
| TCTGGTGCTTGTCACCGTCA+AGG | 0.605234 | 8_3:-7143691 | Msa1273410:CDS |
| GTGAAAGGAGAAGCAAAACA+TGG | 0.650226 | 8_3:-7143777 | Msa1273410:CDS |
| TAAAAGACAGTGCATCAATG+TGG | 0.668011 | 8_3:-7143725 | Msa1273410:CDS |
| TCAGGACCTTGTCTTAACAC+AGG | 0.703356 | 8_3:-7144117 | Msa1273410:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTATTAGGAACATAAATA+AGG | + | chr8_3:7143741-7143760 | None:intergenic | 15.0% |
| !! | TAATAATGAAGCAAATTATT+AGG | + | chr8_3:7143754-7143773 | None:intergenic | 15.0% |
| !!! | ATATGGTTATATTATAGTTT+TGG | + | chr8_3:7144011-7144030 | None:intergenic | 15.0% |
| !! | GATTAATTGTTACATTTATA+TGG | + | chr8_3:7144028-7144047 | None:intergenic | 15.0% |
| !! | AAATGAACAATTATTAATGT+AGG | + | chr8_3:7144081-7144100 | None:intergenic | 15.0% |
| !! | AAATTAAGAACACAAAAATA+TGG | + | chr8_3:7144110-7144129 | None:intergenic | 15.0% |
| !!! | TTTTTCTTTTTTATATGAAG+AGG | - | chr8_3:7144139-7144158 | Msa1273410:CDS | 15.0% |
| !! | AATTATTAGGAACATAAATA+AGG | + | chr8_3:7143741-7143760 | None:intergenic | 15.0% |
| !! | TAATAATGAAGCAAATTATT+AGG | + | chr8_3:7143754-7143773 | None:intergenic | 15.0% |
| !!! | ATATGGTTATATTATAGTTT+TGG | + | chr8_3:7144011-7144030 | None:intergenic | 15.0% |
| !! | GATTAATTGTTACATTTATA+TGG | + | chr8_3:7144028-7144047 | None:intergenic | 15.0% |
| !! | AAATGAACAATTATTAATGT+AGG | + | chr8_3:7144081-7144100 | None:intergenic | 15.0% |
| !! | AAATTAAGAACACAAAAATA+TGG | + | chr8_3:7144110-7144129 | None:intergenic | 15.0% |
| !!! | TTTTTCTTTTTTATATGAAG+AGG | - | chr8_3:7144139-7144158 | Msa1273410:CDS | 15.0% |
| !! | AATGTTAACAATATACTCTT+TGG | + | chr8_3:7143963-7143982 | None:intergenic | 20.0% |
| !! | AATGTTAACAATATACTCTT+TGG | + | chr8_3:7143963-7143982 | None:intergenic | 20.0% |
| ! | AACAATTAATCAGATACATG+TGG | - | chr8_3:7144037-7144056 | Msa1273410:CDS | 25.0% |
| !!! | TTCTTTTTTATATGAAGAGG+TGG | - | chr8_3:7144142-7144161 | Msa1273410:CDS | 25.0% |
| ! | AACAATTAATCAGATACATG+TGG | - | chr8_3:7144037-7144056 | Msa1273410:CDS | 25.0% |
| !!! | TTCTTTTTTATATGAAGAGG+TGG | - | chr8_3:7144142-7144161 | Msa1273410:CDS | 25.0% |
| !! | TGATTAATTTGTACCAGATG+TGG | + | chr8_3:7143684-7143703 | None:intergenic | 30.0% |
| ! | TTTTTGCTTTCTATGTGTAG+AGG | - | chr8_3:7143776-7143795 | Msa1273410:CDS | 30.0% |
| GTCAAAAACGAAGTAAAACA+TGG | - | chr8_3:7143814-7143833 | Msa1273410:intron | 30.0% | |
| TAAAAGACAATGCGTTAATG+TGG | - | chr8_3:7143866-7143885 | Msa1273410:intron | 30.0% | |
| !! | TGATTAATTTGTACCAGATG+TGG | + | chr8_3:7143684-7143703 | None:intergenic | 30.0% |
| ! | TTTTTGCTTTCTATGTGTAG+AGG | - | chr8_3:7143776-7143795 | Msa1273410:CDS | 30.0% |
| GTCAAAAACGAAGTAAAACA+TGG | - | chr8_3:7143814-7143833 | Msa1273410:intron | 30.0% | |
| TAAAAGACAATGCGTTAATG+TGG | - | chr8_3:7143866-7143885 | Msa1273410:intron | 30.0% | |
| GCTGTAGAATTTACTAACAG+AGG | + | chr8_3:7143624-7143643 | None:intergenic | 35.0% | |
| AGATGTGGAAATAAGGAGTT+GGG | + | chr8_3:7143669-7143688 | None:intergenic | 35.0% | |
| TTGTACCAGATGTGGAAATA+AGG | + | chr8_3:7143676-7143695 | None:intergenic | 35.0% | |
| AAACGAAGTAAAACATGGTC+AGG | - | chr8_3:7143819-7143838 | Msa1273410:intron | 35.0% | |
| TGGAACCAAAACTTTGTGAA+AGG | - | chr8_3:7144162-7144181 | Msa1273410:intron | 35.0% | |
| ! | CTTCTCCTTTCACAAAGTTT+TGG | + | chr8_3:7144170-7144189 | None:intergenic | 35.0% |
| CAATTTCCTGAGATAAGACA+AGG | + | chr8_3:7144209-7144228 | None:intergenic | 35.0% | |
| TAAAAGACAGTGCATCAATG+TGG | - | chr8_3:7144229-7144248 | Msa1273410:intron | 35.0% | |
| GCTGTAGAATTTACTAACAG+AGG | + | chr8_3:7143624-7143643 | None:intergenic | 35.0% | |
| AGATGTGGAAATAAGGAGTT+GGG | + | chr8_3:7143669-7143688 | None:intergenic | 35.0% | |
| TTGTACCAGATGTGGAAATA+AGG | + | chr8_3:7143676-7143695 | None:intergenic | 35.0% | |
| AAACGAAGTAAAACATGGTC+AGG | - | chr8_3:7143819-7143838 | Msa1273410:intron | 35.0% | |
| TGGAACCAAAACTTTGTGAA+AGG | - | chr8_3:7144162-7144181 | Msa1273410:intron | 35.0% | |
| ! | CTTCTCCTTTCACAAAGTTT+TGG | + | chr8_3:7144170-7144189 | None:intergenic | 35.0% |
| CAATTTCCTGAGATAAGACA+AGG | + | chr8_3:7144209-7144228 | None:intergenic | 35.0% | |
| TAAAAGACAGTGCATCAATG+TGG | - | chr8_3:7144229-7144248 | Msa1273410:intron | 35.0% | |
| CAGATGTGGAAATAAGGAGT+TGG | + | chr8_3:7143670-7143689 | None:intergenic | 40.0% | |
| ! | CAACTCCTTATTTCCACATC+TGG | - | chr8_3:7143668-7143687 | Msa1273410:CDS | 40.0% |
| TTGCTTTCTATGTGTAGAGG+TGG | - | chr8_3:7143779-7143798 | Msa1273410:CDS | 40.0% | |
| CAGTTTCCTGTGTTAAGACA+AGG | + | chr8_3:7143846-7143865 | None:intergenic | 40.0% | |
| !!! | AATGTGGAGCATGCTACTTT+TGG | - | chr8_3:7143882-7143901 | Msa1273410:intron | 40.0% |
| GTGAAAGGAGAAGCAAAACA+TGG | - | chr8_3:7144177-7144196 | Msa1273410:intron | 40.0% | |
| AAGCAAAACCAATGCCTTGA+CGG | + | chr8_3:7144280-7144299 | None:intergenic | 40.0% | |
| CAGATGTGGAAATAAGGAGT+TGG | + | chr8_3:7143670-7143689 | None:intergenic | 40.0% | |
| ! | CAACTCCTTATTTCCACATC+TGG | - | chr8_3:7143668-7143687 | Msa1273410:CDS | 40.0% |
| TTGCTTTCTATGTGTAGAGG+TGG | - | chr8_3:7143779-7143798 | Msa1273410:CDS | 40.0% | |
| CAGTTTCCTGTGTTAAGACA+AGG | + | chr8_3:7143846-7143865 | None:intergenic | 40.0% | |
| !!! | AATGTGGAGCATGCTACTTT+TGG | - | chr8_3:7143882-7143901 | Msa1273410:intron | 40.0% |
| GTGAAAGGAGAAGCAAAACA+TGG | - | chr8_3:7144177-7144196 | Msa1273410:intron | 40.0% | |
| AAGCAAAACCAATGCCTTGA+CGG | + | chr8_3:7144280-7144299 | None:intergenic | 40.0% | |
| TGGAAATAAGGAGTTGGGCA+AGG | + | chr8_3:7143664-7143683 | None:intergenic | 45.0% | |
| TCAGGACCTTGTCTTAACAC+AGG | - | chr8_3:7143837-7143856 | Msa1273410:intron | 45.0% | |
| !! | TTTGGTGCTTGTCATCGTCA+AGG | - | chr8_3:7143900-7143919 | Msa1273410:intron | 45.0% |
| AGGAGAAGCAAAACATGGTC+AGG | - | chr8_3:7144182-7144201 | Msa1273410:intron | 45.0% | |
| TCAGGACCTTGTCTTATCTC+AGG | - | chr8_3:7144200-7144219 | Msa1273410:intron | 45.0% | |
| AATGTGGAGCATGCAACTTC+TGG | - | chr8_3:7144245-7144264 | Msa1273410:intron | 45.0% | |
| TGGAAATAAGGAGTTGGGCA+AGG | + | chr8_3:7143664-7143683 | None:intergenic | 45.0% | |
| TCAGGACCTTGTCTTAACAC+AGG | - | chr8_3:7143837-7143856 | Msa1273410:intron | 45.0% | |
| !! | TTTGGTGCTTGTCATCGTCA+AGG | - | chr8_3:7143900-7143919 | Msa1273410:intron | 45.0% |
| AGGAGAAGCAAAACATGGTC+AGG | - | chr8_3:7144182-7144201 | Msa1273410:intron | 45.0% | |
| TCAGGACCTTGTCTTATCTC+AGG | - | chr8_3:7144200-7144219 | Msa1273410:intron | 45.0% | |
| AATGTGGAGCATGCAACTTC+TGG | - | chr8_3:7144245-7144264 | Msa1273410:intron | 45.0% | |
| GCTTGTCATCGTCAAGGCTT+TGG | - | chr8_3:7143906-7143925 | Msa1273410:intron | 50.0% | |
| GCTTGTCATCGTCAAGGCTT+TGG | - | chr8_3:7143906-7143925 | Msa1273410:intron | 50.0% | |
| TCTGGTGCTTGTCACCGTCA+AGG | - | chr8_3:7144263-7144282 | Msa1273410:intron | 55.0% | |
| GCTTGTCACCGTCAAGGCAT+TGG | - | chr8_3:7144269-7144288 | Msa1273410:intron | 55.0% | |
| TCTGGTGCTTGTCACCGTCA+AGG | - | chr8_3:7144263-7144282 | Msa1273410:intron | 55.0% | |
| GCTTGTCACCGTCAAGGCAT+TGG | - | chr8_3:7144269-7144288 | Msa1273410:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_3 | gene | 7143614 | 7144362 | 7143614 | ID=Msa1273410;Name=Msa1273410 |
| chr8_3 | mRNA | 7143614 | 7144362 | 7143614 | ID=Msa1273410-mRNA-1;Parent=Msa1273410;Name=Msa1273410-mRNA-1;_AED=0.30;_eAED=0.98;_QI=0|0|0|1|1|1|3|0|128 |
| chr8_3 | exon | 7144351 | 7144362 | 7144351 | ID=Msa1273410-mRNA-1:exon:2804;Parent=Msa1273410-mRNA-1 |
| chr8_3 | exon | 7144010 | 7144180 | 7144010 | ID=Msa1273410-mRNA-1:exon:2803;Parent=Msa1273410-mRNA-1 |
| chr8_3 | exon | 7143614 | 7143817 | 7143614 | ID=Msa1273410-mRNA-1:exon:2802;Parent=Msa1273410-mRNA-1 |
| chr8_3 | CDS | 7144351 | 7144362 | 7144351 | ID=Msa1273410-mRNA-1:cds;Parent=Msa1273410-mRNA-1 |
| chr8_3 | CDS | 7144010 | 7144180 | 7144010 | ID=Msa1273410-mRNA-1:cds;Parent=Msa1273410-mRNA-1 |
| chr8_3 | CDS | 7143614 | 7143817 | 7143614 | ID=Msa1273410-mRNA-1:cds;Parent=Msa1273410-mRNA-1 |
| Gene Sequence |
| Protein sequence |
