Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1347270 | KEH18157.1 | 93.671 | 79 | 5 | 0 | 1 | 79 | 1 | 79 | 2.09e-46 | 152 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1347270 | sp|P82787|DEF19_ARATH | 51.351 | 74 | 33 | 1 | 9 | 79 | 5 | 78 | 1.87e-20 | 80.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1347270 | A0A072TL50 | 93.671 | 79 | 5 | 0 | 1 | 79 | 1 | 79 | 9.98e-47 | 152 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0010150 | Msa1347270 | 0.804024 | 2.633986e-49 | -8.615850e-47 |
| Msa0059880 | Msa1347270 | 0.812404 | 4.312850e-51 | -8.615850e-47 |
| Msa1347270 | Msa1378160 | 0.865354 | 6.275332e-65 | -8.615850e-47 |
| Msa1347270 | Msa1378180 | 0.854379 | 1.280437e-61 | -8.615850e-47 |
| Msa0786940 | Msa1347270 | 0.819908 | 9.069576e-53 | -8.615850e-47 |
| Msa0822410 | Msa1347270 | 0.801435 | 9.015171e-49 | -8.615850e-47 |
| Msa0150910 | Msa1347270 | 0.809852 | 1.542036e-50 | -8.615850e-47 |
| Msa1230790 | Msa1347270 | 0.872742 | 2.517033e-67 | -8.615850e-47 |
| Msa1273410 | Msa1347270 | 0.822315 | 2.529528e-53 | -8.615850e-47 |
| Msa1323030 | Msa1347270 | 0.801042 | 1.085150e-48 | -8.615850e-47 |
| Msa1006170 | Msa1347270 | 0.801600 | 8.342892e-49 | -8.615850e-47 |
| Msa1018590 | Msa1347270 | 0.803256 | 3.801864e-49 | -8.615850e-47 |
| Msa1120680 | Msa1347270 | 0.854925 | 8.897097e-62 | -8.615850e-47 |
| Msa1156240 | Msa1347270 | 0.817052 | 4.027365e-52 | -8.615850e-47 |
| Msa0580100 | Msa1347270 | 0.803039 | 4.217174e-49 | -8.615850e-47 |
| Msa0854840 | Msa1347270 | 0.802810 | 4.700641e-49 | -8.615850e-47 |
| Msa0861730 | Msa1347270 | 0.823378 | 1.430688e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1347270 | MtrunA17_Chr8g0339701 | 93.671 | 79 | 5 | 0 | 1 | 79 | 1 | 79 | 1.92e-50 | 152 |
| Msa1347270 | MtrunA17_Chr8g0339681 | 89.873 | 79 | 8 | 0 | 1 | 79 | 1 | 79 | 7.83e-49 | 150 |
| Msa1347270 | MtrunA17_Chr8g0339681 | 64.706 | 68 | 24 | 0 | 12 | 79 | 69 | 136 | 6.06e-28 | 97.8 |
| Msa1347270 | MtrunA17_Chr8g0339721 | 67.089 | 79 | 26 | 0 | 1 | 79 | 1 | 79 | 2.18e-29 | 99.8 |
| Msa1347270 | MtrunA17_Chr8g0339691 | 76.923 | 52 | 12 | 0 | 27 | 78 | 1 | 52 | 1.60e-26 | 92.8 |
| Msa1347270 | MtrunA17_Chr8g0339741 | 53.750 | 80 | 36 | 1 | 1 | 79 | 1 | 80 | 6.48e-24 | 85.9 |
| Msa1347270 | MtrunA17_Chr8g0339711 | 50.000 | 80 | 38 | 2 | 1 | 79 | 1 | 79 | 2.23e-20 | 77.0 |
| Msa1347270 | MtrunA17_Chr8g0339751 | 56.962 | 79 | 33 | 1 | 1 | 79 | 1 | 78 | 4.72e-20 | 76.3 |
| Msa1347270 | MtrunA17_Chr3g0084701 | 51.899 | 79 | 36 | 1 | 1 | 79 | 1 | 77 | 1.50e-19 | 74.7 |
| Msa1347270 | MtrunA17_Chr8g0339651 | 46.835 | 79 | 37 | 2 | 4 | 79 | 13 | 89 | 1.14e-18 | 72.8 |
| Msa1347270 | MtrunA17_Chr7g0260561 | 46.341 | 82 | 38 | 4 | 1 | 79 | 1 | 79 | 6.53e-18 | 70.9 |
| Msa1347270 | MtrunA17_Chr8g0357271 | 50.617 | 81 | 35 | 4 | 1 | 79 | 1 | 78 | 2.50e-17 | 69.3 |
| Msa1347270 | MtrunA17_Chr8g0345161 | 48.052 | 77 | 36 | 2 | 3 | 79 | 4 | 76 | 1.52e-16 | 67.0 |
| Msa1347270 | MtrunA17_Chr8g0339481 | 45.000 | 80 | 43 | 1 | 1 | 79 | 1 | 80 | 1.93e-14 | 62.0 |
| Msa1347270 | MtrunA17_Chr8g0359831 | 42.857 | 77 | 39 | 2 | 4 | 79 | 5 | 77 | 1.33e-13 | 59.7 |
| Msa1347270 | MtrunA17_Chr8g0339501 | 46.875 | 64 | 33 | 1 | 1 | 63 | 1 | 64 | 2.87e-13 | 60.1 |
| Msa1347270 | MtrunA17_Chr8g0339571 | 46.835 | 79 | 39 | 2 | 1 | 79 | 9 | 84 | 1.86e-12 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1347270 | AT1G19610.1 | 51.351 | 74 | 33 | 1 | 9 | 79 | 5 | 78 | 1.90e-21 | 80.1 |
| Msa1347270 | AT2G26020.1 | 37.975 | 79 | 44 | 1 | 6 | 79 | 2 | 80 | 2.31e-12 | 57.0 |
| Msa1347270 | AT5G44420.1 | 35.443 | 79 | 46 | 1 | 6 | 79 | 2 | 80 | 3.20e-11 | 53.9 |
| Msa1347270 | AT5G44430.1 | 40.506 | 79 | 42 | 2 | 6 | 79 | 2 | 80 | 4.24e-11 | 53.9 |
Find 15 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCTCCTTATTTCCACATC+TGG | 0.358521 | tig0009642:+39298 | Msa1347270:CDS |
| TTGTACCAGATGTGGAAATA+AGG | 0.362903 | tig0009642:-39303 | None:intergenic |
| GCTTGTCACCGTCAAGGCTT+TGG | 0.378692 | tig0009642:+39521 | Msa1347270:CDS |
| GATGTGGAGCAGGCTACTTT+TGG | 0.459196 | tig0009642:+39497 | Msa1347270:CDS |
| AAGCAAAACCAAAGCCTTGA+CGG | 0.464247 | tig0009642:-39529 | None:intergenic |
| GGTGTAAAATTTACTAGTAG+AGG | 0.495463 | tig0009642:-39251 | None:intergenic |
| AAACGAAGTAAAACGTGGTC+AGG | 0.502910 | tig0009642:+39434 | Msa1347270:CDS |
| TGATTAGTTTGTACCAGATG+TGG | 0.519499 | tig0009642:-39311 | None:intergenic |
| CAGTTTCCTGTGTTAAGACA+AGG | 0.529102 | tig0009642:-39458 | None:intergenic |
| ACAATGCGTTGATGTGGAGC+AGG | 0.533581 | tig0009642:+39487 | Msa1347270:CDS |
| AGATGTGGAAATAAGGAGAA+AGG | 0.535384 | tig0009642:-39296 | None:intergenic |
| TTTGGTGCTTGTCACCGTCA+AGG | 0.553812 | tig0009642:+39515 | Msa1347270:CDS |
| TAAAAGACAATGCGTTGATG+TGG | 0.623871 | tig0009642:+39481 | Msa1347270:CDS |
| TGGAAATAAGGAGAAAGGCA+AGG | 0.649319 | tig0009642:-39291 | None:intergenic |
| TCAGGACCTTGTCTTAACAC+AGG | 0.703356 | tig0009642:+39452 | Msa1347270:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATGAACAGTTATTAATAT+AGG | - | tig0009642:39342-39361 | None:intergenic | 15.0% |
| !!! | TTTATTTTTTTTATGTGTAG+AGG | + | tig0009642:39391-39410 | Msa1347270:intron | 15.0% |
| ! | ACAAGTCAAGAACATAAATA+AGG | - | tig0009642:39371-39390 | None:intergenic | 25.0% |
| !!! | ATTTTTTTTATGTGTAGAGG+TGG | + | tig0009642:39394-39413 | Msa1347270:intron | 25.0% |
| GGTGTAAAATTTACTAGTAG+AGG | - | tig0009642:39254-39273 | None:intergenic | 30.0% | |
| AAGGCAGACAAAAACAAAGA+TGG | - | tig0009642:39275-39294 | None:intergenic | 35.0% | |
| AGATGTGGAAATAAGGAGAA+AGG | - | tig0009642:39299-39318 | None:intergenic | 35.0% | |
| ! | TTTCTCCTTATTTCCACATC+TGG | + | tig0009642:39298-39317 | Msa1347270:CDS | 35.0% |
| TTGTACCAGATGTGGAAATA+AGG | - | tig0009642:39306-39325 | None:intergenic | 35.0% | |
| !! | TGATTAGTTTGTACCAGATG+TGG | - | tig0009642:39314-39333 | None:intergenic | 35.0% |
| GTCAAAAACGAAGTAAAACG+TGG | + | tig0009642:39429-39448 | Msa1347270:CDS | 35.0% | |
| ! | TAAAAGACAATGCGTTGATG+TGG | + | tig0009642:39481-39500 | Msa1347270:CDS | 35.0% |
| TGGAAATAAGGAGAAAGGCA+AGG | - | tig0009642:39294-39313 | None:intergenic | 40.0% | |
| AAACGAAGTAAAACGTGGTC+AGG | + | tig0009642:39434-39453 | Msa1347270:CDS | 40.0% | |
| CAGTTTCCTGTGTTAAGACA+AGG | - | tig0009642:39461-39480 | None:intergenic | 40.0% | |
| AAGCAAAACCAAAGCCTTGA+CGG | - | tig0009642:39532-39551 | None:intergenic | 40.0% | |
| TCAGGACCTTGTCTTAACAC+AGG | + | tig0009642:39452-39471 | Msa1347270:CDS | 45.0% | |
| ! | ACAATGCGTTGATGTGGAGC+AGG | + | tig0009642:39487-39506 | Msa1347270:CDS | 50.0% |
| !! | GATGTGGAGCAGGCTACTTT+TGG | + | tig0009642:39497-39516 | Msa1347270:CDS | 50.0% |
| !! | TTTGGTGCTTGTCACCGTCA+AGG | + | tig0009642:39515-39534 | Msa1347270:CDS | 50.0% |
| GCTTGTCACCGTCAAGGCTT+TGG | + | tig0009642:39521-39540 | Msa1347270:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0009642 | gene | 39244 | 39574 | 39244 | ID=Msa1347270;Name=Msa1347270 |
| tig0009642 | mRNA | 39244 | 39574 | 39244 | ID=Msa1347270-mRNA-1;Parent=Msa1347270;Name=Msa1347270-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0|0|0|1|0|0.5|2|0|79 |
| tig0009642 | exon | 39244 | 39319 | 39244 | ID=Msa1347270-mRNA-1:exon:5815;Parent=Msa1347270-mRNA-1 |
| tig0009642 | exon | 39411 | 39574 | 39411 | ID=Msa1347270-mRNA-1:exon:5816;Parent=Msa1347270-mRNA-1 |
| tig0009642 | CDS | 39244 | 39319 | 39244 | ID=Msa1347270-mRNA-1:cds;Parent=Msa1347270-mRNA-1 |
| tig0009642 | CDS | 39411 | 39574 | 39411 | ID=Msa1347270-mRNA-1:cds;Parent=Msa1347270-mRNA-1 |
| Gene Sequence |
| Protein sequence |
