AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1276770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1276770 MtrunA17_Chr8g0344441 100.000 128 0 0 1 128 1 128 3.20e-91 259
Msa1276770 MtrunA17_Chr2g0301051 82.812 128 22 0 1 128 1 128 2.35e-76 222
Msa1276770 MtrunA17_Chr4g0044211 38.356 73 44 1 14 85 7 79 1.97e-12 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1276770 AT3G14080.2 86.719 128 17 0 1 128 1 128 1.72e-80 233
Msa1276770 AT3G14080.1 86.719 128 17 0 1 128 1 128 1.72e-80 233
Msa1276770 AT1G19120.1 81.250 128 24 0 1 128 1 128 2.07e-74 217
Msa1276770 AT3G14080.4 83.621 116 17 1 15 128 3 118 1.43e-65 194
Msa1276770 AT3G14080.3 90.123 81 8 0 1 81 1 81 5.61e-50 154
Msa1276770 AT1G65700.2 38.667 75 45 1 12 85 4 78 5.58e-12 58.2
Msa1276770 AT1G65700.1 38.667 75 45 1 12 85 4 78 5.58e-12 58.2

Find 62 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGTTAATTTCTGGAAATTT+AGG 0.147955 8_3:+11621369 Msa1276770:five_prime_UTR
GAAGCGAAAGAGTGAAAATT+TGG 0.161483 8_3:-11621267 None:intergenic
TAGATCGAAATCACAATAAA+AGG 0.191374 8_3:-11621326 None:intergenic
AAGTGGGTGATTTGAGAAAA+AGG 0.224442 8_3:-11621300 None:intergenic
TCTTCTTTATATATAGGAAT+TGG 0.262226 8_3:+11621400 Msa1276770:five_prime_UTR
AGGGATGCTTCAGAACTAAA+AGG 0.302591 8_3:+11624164 Msa1276770:CDS
GGGGAAAATGTTGTTCTAAT+TGG 0.311566 8_3:+11622454 Msa1276770:CDS
AAGCGAAAGAGTGAAAATTT+GGG 0.332275 8_3:-11621266 None:intergenic
CCTAAATTTCCAGAAATTAA+CGG 0.358389 8_3:-11621369 None:intergenic
CTTCTTTATATATAGGAATT+GGG 0.377863 8_3:+11621401 Msa1276770:five_prime_UTR
TTGTTCTTCTGTGATATTGT+TGG 0.380565 8_3:+11624292 Msa1276770:three_prime_UTR
GGAATTCAATCTCAGTGAAT+AGG 0.381686 8_3:+11621422 Msa1276770:five_prime_UTR
ATCACAATAAAAGGGTTAAG+TGG 0.445738 8_3:-11621317 None:intergenic
GTAGAAAGGTAGAGATCCTC+AGG 0.460456 8_3:-11621508 None:intergenic
TATCTGGTTTGGAAATGAAA+AGG 0.463066 8_3:+11624462 Msa1276770:three_prime_UTR
GATATTCAAAATGTCAGTTA+TGG 0.463126 8_3:-11624443 None:intergenic
TAACGGAATGAAAAGAACTA+AGG 0.464650 8_3:-11621352 None:intergenic
GGGAGGGATGAAAACATTGT+TGG 0.466313 8_3:+11624322 Msa1276770:three_prime_UTR
AGATCGAAATCACAATAAAA+GGG 0.480054 8_3:-11621325 None:intergenic
AAGAATGGAGTTCCTTGACT+TGG 0.481713 8_3:+11624199 Msa1276770:CDS
GCAGCTAACGCTGTTCTTGA+GGG 0.491345 8_3:+11622364 Msa1276770:intron
GTGTTCCTACAGAAGAAATC+AGG 0.501365 8_3:+11622605 Msa1276770:CDS
TTCTTGAGGGTGCCTGTGAG+AGG 0.503890 8_3:+11622377 Msa1276770:CDS
GTAGGAACACGCGTCATATG+TGG 0.510896 8_3:-11622592 None:intergenic
TGCAGCTAACGCTGTTCTTG+AGG 0.511473 8_3:+11622363 Msa1276770:intron
TATGTCTTGGGCTGCTCCTG+AGG 0.516307 8_3:+11621492 Msa1276770:exon
AACTAGCAAGTGAAGTAGAA+AGG 0.522695 8_3:-11621522 None:intergenic
CTATATTGTGACATCCCTCT+AGG 0.524354 8_3:+11622415 Msa1276770:CDS
ACGAGATGGTAGAAAGCTCA+TGG 0.524959 8_3:+11622240 Msa1276770:CDS
GATATTGCAGGCACAGAAAG+TGG 0.530521 8_3:+11624139 Msa1276770:intron
CAATCTCAGTGAATAGGTTG+TGG 0.530649 8_3:+11621428 Msa1276770:five_prime_UTR
AAGAACAAAATAAAATAACT+TGG 0.534155 8_3:-11624277 None:intergenic
GGGATGCTTCAGAACTAAAA+GGG 0.534576 8_3:+11624165 Msa1276770:CDS
TAGGAACACGCGTCATATGT+GGG 0.543008 8_3:-11622591 None:intergenic
AAGAGCCGCTGTAATATTTG+CGG 0.553134 8_3:-11624252 None:intergenic
TCACAATAAAAGGGTTAAGT+GGG 0.553685 8_3:-11621316 None:intergenic
GCAGGCACAGAAAGTGGAAA+GGG 0.554246 8_3:+11624145 Msa1276770:intron
GCCTGTGAGAGGGTAATTGT+TGG 0.562739 8_3:+11622388 Msa1276770:CDS
AGCGAAAGAGTGAAAATTTG+GGG 0.565663 8_3:-11621265 None:intergenic
CTAGGTCTTTATGTAATCCG+TGG 0.570170 8_3:+11622433 Msa1276770:CDS
TGTTGTTCTAATTGGTGAGA+TGG 0.571580 8_3:+11622462 Msa1276770:CDS
CTTCTTATATTGCTACGAGA+TGG 0.573728 8_3:+11622226 Msa1276770:CDS
TTGACAAGAAAAGAAAGCAA+AGG 0.583127 8_3:-11624377 None:intergenic
TATATCACAAAATTCACCAA+TGG 0.584239 8_3:-11621456 None:intergenic
CGAGATGGTAGAAAGCTCAT+GGG 0.590247 8_3:+11622241 Msa1276770:CDS
TAGGTCTTTATGTAATCCGT+GGG 0.590765 8_3:+11622434 Msa1276770:CDS
TCTTGAGGGTGCCTGTGAGA+GGG 0.598880 8_3:+11622378 Msa1276770:CDS
ACCCTCCTGATTTCTTCTGT+AGG 0.607048 8_3:-11622610 None:intergenic
ACCAACAATTACCCTCTCAC+AGG 0.608128 8_3:-11622389 None:intergenic
TGCAGGCACAGAAAGTGGAA+AGG 0.609461 8_3:+11624144 Msa1276770:intron
ATAGGTTGTGGCAATGCCAT+TGG 0.610982 8_3:+11621440 Msa1276770:five_prime_UTR
TATTTGCGGATGAGCGAGTG+TGG 0.613060 8_3:-11624238 None:intergenic
AGGAACACGCGTCATATGTG+GGG 0.634207 8_3:-11622590 None:intergenic
TTCCTACAGAAGAAATCAGG+AGG 0.637433 8_3:+11622608 Msa1276770:CDS
TTAATCACTAGTCCAAGTCA+AGG 0.651795 8_3:-11624211 None:intergenic
CTCATCCGCAAATATTACAG+CGG 0.668106 8_3:+11624247 Msa1276770:three_prime_UTR
GGATTACATAAAGACCTAGA+GGG 0.677541 8_3:-11622429 None:intergenic
GGAACACGCGTCATATGTGG+GGG 0.684221 8_3:-11622589 None:intergenic
CGGATTACATAAAGACCTAG+AGG 0.700189 8_3:-11622430 None:intergenic
TCCTACAGAAGAAATCAGGA+GGG 0.714873 8_3:+11622609 Msa1276770:CDS
GAGATGGTAGAAAGCTCATG+GGG 0.721011 8_3:+11622242 Msa1276770:CDS
AGGTCTTTATGTAATCCGTG+GGG 0.758020 8_3:+11622435 Msa1276770:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTCTTCTTTATATAT+AGG + chr8_3:11621394-11621413 Msa1276770:five_prime_UTR 10.0%
!! AAAAATCATAATTATGTCTT+GGG + chr8_3:11621480-11621499 Msa1276770:exon 15.0%
!! ATTAGACAAAAATAAAAACA+TGG - chr8_3:11621587-11621606 None:intergenic 15.0%
!!! ATCATAAAGAAGTTTAATTT+AGG - chr8_3:11622643-11622662 None:intergenic 15.0%
!! TATTAATATACGACAATAAT+AGG - chr8_3:11622751-11622770 None:intergenic 15.0%
!! TTATGTATTATCAATGTAAA+TGG - chr8_3:11623785-11623804 None:intergenic 15.0%
!!! TAAGATTTTATTTTTTAAGC+AGG - chr8_3:11623899-11623918 None:intergenic 15.0%
!!! TTGATATTTTTGTTATGATT+TGG + chr8_3:11624041-11624060 Msa1276770:intron 15.0%
!! AAGAACAAAATAAAATAACT+TGG - chr8_3:11624280-11624299 None:intergenic 15.0%
!! TCTTCTTTATATATAGGAAT+TGG + chr8_3:11621400-11621419 Msa1276770:five_prime_UTR 20.0%
!! CTTCTTTATATATAGGAATT+GGG + chr8_3:11621401-11621420 Msa1276770:five_prime_UTR 20.0%
!! GAAAAATCATAATTATGTCT+TGG + chr8_3:11621479-11621498 Msa1276770:exon 20.0%
!! AAGAAACATATAGATATGTT+TGG + chr8_3:11621836-11621855 Msa1276770:intron 20.0%
!! TTATTAATGGTAATTAAGCT+TGG + chr8_3:11622126-11622145 Msa1276770:intron 20.0%
!! TATTAATGGTAATTAAGCTT+GGG + chr8_3:11622127-11622146 Msa1276770:intron 20.0%
!!! GAAATAGTTTTAAATGCAAA+GGG - chr8_3:11622506-11622525 None:intergenic 20.0%
!!! AGAAATAGTTTTAAATGCAA+AGG - chr8_3:11622507-11622526 None:intergenic 20.0%
!! AAGTTAGATTAGTAATGTTT+GGG + chr8_3:11622874-11622893 Msa1276770:intron 20.0%
!!! AAAAGTACAATAATTGTGTT+TGG - chr8_3:11622974-11622993 None:intergenic 20.0%
!! AAATAAAGTGGATAAACTAA+TGG + chr8_3:11623230-11623249 Msa1276770:intron 20.0%
!!! TGAAATTTAGGATTAATTGA+AGG - chr8_3:11623871-11623890 None:intergenic 20.0%
!!! TTTTTGTTATGATTTGGAAT+TGG + chr8_3:11624047-11624066 Msa1276770:intron 20.0%
!!! AAATTTGGGGATTTTGAAAT+GGG - chr8_3:11621255-11621274 None:intergenic 25.0%
!!! AAAATTTGGGGATTTTGAAA+TGG - chr8_3:11621256-11621275 None:intergenic 25.0%
! AGATCGAAATCACAATAAAA+GGG - chr8_3:11621328-11621347 None:intergenic 25.0%
! TAGATCGAAATCACAATAAA+AGG - chr8_3:11621329-11621348 None:intergenic 25.0%
! CCTAAATTTCCAGAAATTAA+CGG - chr8_3:11621372-11621391 None:intergenic 25.0%
! TATATCACAAAATTCACCAA+TGG - chr8_3:11621459-11621478 None:intergenic 25.0%
! ATAATTAAAAAAGACCCGTT+TGG + chr8_3:11621643-11621662 Msa1276770:intron 25.0%
! ATAAATAAGTCAATCCAAAC+AGG - chr8_3:11621713-11621732 None:intergenic 25.0%
! TATCTATATGTTTCTTGTCA+AGG - chr8_3:11621832-11621851 None:intergenic 25.0%
! TGATGAAAACCAGTTATTAA+TGG + chr8_3:11622113-11622132 Msa1276770:intron 25.0%
! GCTTAATTACCATTAATAAC+TGG - chr8_3:11622125-11622144 None:intergenic 25.0%
! TCAGTAATAGAACATAAGAT+TGG - chr8_3:11622189-11622208 None:intergenic 25.0%
! GAAGTTAGATTAGTAATGTT+TGG + chr8_3:11622873-11622892 Msa1276770:intron 25.0%
! GTGATATCTACTAATTGTTA+TGG + chr8_3:11622998-11623017 Msa1276770:intron 25.0%
! GTGAAATAAGGGTAAATTAT+AGG + chr8_3:11623037-11623056 Msa1276770:intron 25.0%
!! TGCATACTTCATAATCATTT+TGG + chr8_3:11623253-11623272 Msa1276770:intron 25.0%
!! AGATGTTTTCTTTTTGTAGT+TGG + chr8_3:11623366-11623385 Msa1276770:intron 25.0%
!!! ATTTTGATTTCGATACTACT+TGG + chr8_3:11623432-11623451 Msa1276770:intron 25.0%
!!! TTTTGATTTCGATACTACTT+GGG + chr8_3:11623433-11623452 Msa1276770:intron 25.0%
! TAACTAATTAGCTACAACTA+AGG - chr8_3:11623525-11623544 None:intergenic 25.0%
! CAGAATACTTTATACATTGA+AGG + chr8_3:11623722-11623741 Msa1276770:intron 25.0%
! GAAACAAGATAATCCAAAAT+TGG + chr8_3:11623839-11623858 Msa1276770:intron 25.0%
!! TTTTATTCAAGCAATAACAG+TGG + chr8_3:11624110-11624129 Msa1276770:intron 25.0%
! GATATTCAAAATGTCAGTTA+TGG - chr8_3:11624446-11624465 None:intergenic 25.0%
!! TAACTGACATTTTGAATATC+TGG + chr8_3:11624446-11624465 Msa1276770:three_prime_UTR 25.0%
AAGCGAAAGAGTGAAAATTT+GGG - chr8_3:11621269-11621288 None:intergenic 30.0%
TCACAATAAAAGGGTTAAGT+GGG - chr8_3:11621319-11621338 None:intergenic 30.0%
ATCACAATAAAAGGGTTAAG+TGG - chr8_3:11621320-11621339 None:intergenic 30.0%
TAACGGAATGAAAAGAACTA+AGG - chr8_3:11621355-11621374 None:intergenic 30.0%
! CTTTTCATTCCGTTAATTTC+TGG + chr8_3:11621360-11621379 Msa1276770:five_prime_UTR 30.0%
! CCGTTAATTTCTGGAAATTT+AGG + chr8_3:11621369-11621388 Msa1276770:five_prime_UTR 30.0%
CAGCTTATAAGCTATAATCA+AGG - chr8_3:11621621-11621640 None:intergenic 30.0%
CTTGATTATAGCTTATAAGC+TGG + chr8_3:11621619-11621638 Msa1276770:intron 30.0%
AATAAGTTCGTGTTACCAAA+CGG - chr8_3:11621661-11621680 None:intergenic 30.0%
TTTCTTGTCAAGGTAGTTTA+CGG - chr8_3:11621822-11621841 None:intergenic 30.0%
TACCTATCAACTTAAGCTTT+GGG + chr8_3:11621915-11621934 Msa1276770:intron 30.0%
ACATCCTGAAAATTGAAAGA+CGG - chr8_3:11622553-11622572 None:intergenic 30.0%
! CATTGTATTGCTTTAAGTGA+GGG - chr8_3:11622699-11622718 None:intergenic 30.0%
CATTGTTTGTAAAGAGATGT+AGG - chr8_3:11622779-11622798 None:intergenic 30.0%
CTACATCTCTTTACAAACAA+TGG + chr8_3:11622777-11622796 Msa1276770:intron 30.0%
! CAGATTTAGAGTTACAGAAT+TGG + chr8_3:11622903-11622922 Msa1276770:intron 30.0%
! GTTACAGAATTGGCTTAAAT+AGG + chr8_3:11622913-11622932 Msa1276770:intron 30.0%
ATTGTTATGGTAAGTAACCT+TGG + chr8_3:11623011-11623030 Msa1276770:intron 30.0%
TAGGATTATTACGATGATGA+CGG + chr8_3:11623056-11623075 Msa1276770:intron 30.0%
GCTAGTACTACTACTTATTA+GGG + chr8_3:11623335-11623354 Msa1276770:intron 30.0%
! TGCTTCGTAGTTATGATTTT+TGG + chr8_3:11623692-11623711 Msa1276770:intron 30.0%
ATGTAAATGGTCGAGTTATA+TGG - chr8_3:11623772-11623791 None:intergenic 30.0%
TCAATGTAGACATGTTAAAG+AGG - chr8_3:11623821-11623840 None:intergenic 30.0%
! TTGAAGGATGTATCCAATTT+TGG - chr8_3:11623855-11623874 None:intergenic 30.0%
AAGCAGGATTCTTGAAATTT+AGG - chr8_3:11623883-11623902 None:intergenic 30.0%
TTGTTCTTCTGTGATATTGT+TGG + chr8_3:11624292-11624311 Msa1276770:three_prime_UTR 30.0%
!!! TGTGATATTGTTGGTTTTCA+TGG + chr8_3:11624301-11624320 Msa1276770:three_prime_UTR 30.0%
!!! GTGATATTGTTGGTTTTCAT+GGG + chr8_3:11624302-11624321 Msa1276770:three_prime_UTR 30.0%
TTGACAAGAAAAGAAAGCAA+AGG - chr8_3:11624380-11624399 None:intergenic 30.0%
! TGCTTTCTTTTCTTGTCAAA+AGG + chr8_3:11624381-11624400 Msa1276770:three_prime_UTR 30.0%
! GACATTTTGAATATCTGGTT+TGG + chr8_3:11624451-11624470 Msa1276770:three_prime_UTR 30.0%
!! TATCTGGTTTGGAAATGAAA+AGG + chr8_3:11624462-11624481 Msa1276770:three_prime_UTR 30.0%
AGCGAAAGAGTGAAAATTTG+GGG - chr8_3:11621268-11621287 None:intergenic 35.0%
GAAGCGAAAGAGTGAAAATT+TGG - chr8_3:11621270-11621289 None:intergenic 35.0%
AGTGGGTGATTTGAGAAAAA+GGG - chr8_3:11621302-11621321 None:intergenic 35.0%
AAGTGGGTGATTTGAGAAAA+AGG - chr8_3:11621303-11621322 None:intergenic 35.0%
GGAATTCAATCTCAGTGAAT+AGG + chr8_3:11621422-11621441 Msa1276770:five_prime_UTR 35.0%
AACTAGCAAGTGAAGTAGAA+AGG - chr8_3:11621525-11621544 None:intergenic 35.0%
ATAAGTTCGTGTTACCAAAC+GGG - chr8_3:11621660-11621679 None:intergenic 35.0%
AAGTAGCTTATTGACCTGTT+TGG + chr8_3:11621696-11621715 Msa1276770:intron 35.0%
TTCTTGTCAAGGTAGTTTAC+GGG - chr8_3:11621821-11621840 None:intergenic 35.0%
CTACCTATCAACTTAAGCTT+TGG + chr8_3:11621914-11621933 Msa1276770:intron 35.0%
ACCTATCAACTTAAGCTTTG+GGG + chr8_3:11621916-11621935 Msa1276770:intron 35.0%
CTTCTTATATTGCTACGAGA+TGG + chr8_3:11622226-11622245 Msa1276770:CDS 35.0%
! CTTCGCTCTTTTGATCAATT+TGG + chr8_3:11622268-11622287 Msa1276770:CDS 35.0%
GTAAAGATTTGACATGCGTT+AGG + chr8_3:11622310-11622329 Msa1276770:intron 35.0%
GTTAGCTGCATGTTATTGAT+AGG - chr8_3:11622353-11622372 None:intergenic 35.0%
GGATTACATAAAGACCTAGA+GGG - chr8_3:11622432-11622451 None:intergenic 35.0%
TAGGTCTTTATGTAATCCGT+GGG + chr8_3:11622434-11622453 Msa1276770:CDS 35.0%
! TTAGAACAACATTTTCCCCA+CGG - chr8_3:11622453-11622472 None:intergenic 35.0%
GGGGAAAATGTTGTTCTAAT+TGG + chr8_3:11622454-11622473 Msa1276770:CDS 35.0%
TGTTGTTCTAATTGGTGAGA+TGG + chr8_3:11622462-11622481 Msa1276770:CDS 35.0%
! ACAACCGTCTTTCAATTTTC+AGG + chr8_3:11622546-11622565 Msa1276770:intron 35.0%
!! GTCTTTCAATTTTCAGGATG+TGG + chr8_3:11622552-11622571 Msa1276770:intron 35.0%
! GCATTGTATTGCTTTAAGTG+AGG - chr8_3:11622700-11622719 None:intergenic 35.0%
TCTACATGAAACAGATTCGT+AGG + chr8_3:11622831-11622850 Msa1276770:intron 35.0%
! ACAGAATTGGCTTAAATAGG+AGG + chr8_3:11622916-11622935 Msa1276770:intron 35.0%
TAGGAGGTTATCTCATAACT+CGG + chr8_3:11622932-11622951 Msa1276770:intron 35.0%
!! TAACCTTGGCTTGTGAAATA+AGG + chr8_3:11623025-11623044 Msa1276770:intron 35.0%
!! AACCTTGGCTTGTGAAATAA+GGG + chr8_3:11623026-11623045 Msa1276770:intron 35.0%
AGGATTATTACGATGATGAC+GGG + chr8_3:11623057-11623076 Msa1276770:intron 35.0%
! GATATAGTGTGGGTTACTTT+AGG - chr8_3:11623138-11623157 None:intergenic 35.0%
GGCTAGTACTACTACTTATT+AGG + chr8_3:11623334-11623353 Msa1276770:intron 35.0%
ACTTGGGAATATAGCTTAGT+TGG + chr8_3:11623449-11623468 Msa1276770:intron 35.0%
AATTAGCTACAACTAAGGAC+AGG - chr8_3:11623520-11623539 None:intergenic 35.0%
TACAATGGACACGTAGTTTA+TGG - chr8_3:11623931-11623950 None:intergenic 35.0%
TGTATGACTGCATGTCAAAA+AGG - chr8_3:11624084-11624103 None:intergenic 35.0%
AGGGACTATGAGAAAAAGAA+TGG + chr8_3:11624184-11624203 Msa1276770:CDS 35.0%
TTAATCACTAGTCCAAGTCA+AGG - chr8_3:11624214-11624233 None:intergenic 35.0%
!!! ATATTGTTGGTTTTCATGGG+AGG + chr8_3:11624305-11624324 Msa1276770:three_prime_UTR 35.0%
!!! TATTGTTGGTTTTCATGGGA+GGG + chr8_3:11624306-11624325 Msa1276770:three_prime_UTR 35.0%
CAATCTCAGTGAATAGGTTG+TGG + chr8_3:11621428-11621447 Msa1276770:five_prime_UTR 40.0%
ATGATCTAACTCCTGAGAGA+TGG + chr8_3:11621871-11621890 Msa1276770:intron 40.0%
! GTTGATAGGTAGCACATGAA+TGG - chr8_3:11621906-11621925 None:intergenic 40.0%
!! ACCCCAAAGCTTAAGTTGAT+AGG - chr8_3:11621920-11621939 None:intergenic 40.0%
ATCTTGCACACAAGTCTTTC+TGG + chr8_3:11622056-11622075 Msa1276770:intron 40.0%
CTATATTGTGACATCCCTCT+AGG + chr8_3:11622415-11622434 Msa1276770:CDS 40.0%
CGGATTACATAAAGACCTAG+AGG - chr8_3:11622433-11622452 None:intergenic 40.0%
CTAGGTCTTTATGTAATCCG+TGG + chr8_3:11622433-11622452 Msa1276770:CDS 40.0%
AGGTCTTTATGTAATCCGTG+GGG + chr8_3:11622435-11622454 Msa1276770:CDS 40.0%
!! TCAATTTTCAGGATGTGGAG+AGG + chr8_3:11622557-11622576 Msa1276770:intron 40.0%
!! CAATTTTCAGGATGTGGAGA+GGG + chr8_3:11622558-11622577 Msa1276770:intron 40.0%
GTGTTCCTACAGAAGAAATC+AGG + chr8_3:11622605-11622624 Msa1276770:CDS 40.0%
TTCCTACAGAAGAAATCAGG+AGG + chr8_3:11622608-11622627 Msa1276770:CDS 40.0%
TCCTACAGAAGAAATCAGGA+GGG + chr8_3:11622609-11622628 Msa1276770:CDS 40.0%
TTTAAGTGAGGGCAAGAACA+AGG - chr8_3:11622688-11622707 None:intergenic 40.0%
! TACCCTTATTTCACAAGCCA+AGG - chr8_3:11623031-11623050 None:intergenic 40.0%
GGTAAAGTCGGATATAGTGT+GGG - chr8_3:11623148-11623167 None:intergenic 40.0%
AGGTAAAGTCGGATATAGTG+TGG - chr8_3:11623149-11623168 None:intergenic 40.0%
CACTATATCCGACTTTACCT+TGG + chr8_3:11623149-11623168 Msa1276770:intron 40.0%
! GATCCACTGCAAGAGTTTTA+CGG + chr8_3:11623171-11623190 Msa1276770:intron 40.0%
GCAACGAGCTAGAAATAAAG+TGG + chr8_3:11623218-11623237 Msa1276770:intron 40.0%
CTTGCCAAATTCTAGCGATT+AGG + chr8_3:11623313-11623332 Msa1276770:intron 40.0%
CTAGCCTAATCGCTAGAATT+TGG - chr8_3:11623320-11623339 None:intergenic 40.0%
CTACAACTAAGGACAGGTAA+CGG - chr8_3:11623514-11623533 None:intergenic 40.0%
TTTCACTCTCTGATTCACCA+GGG + chr8_3:11623617-11623636 Msa1276770:intron 40.0%
CTTACCAATCATGCGTACAA+TGG - chr8_3:11623946-11623965 None:intergenic 40.0%
TGACTGCATGTCAAAAAGGA+CGG - chr8_3:11624080-11624099 None:intergenic 40.0%
CAGTGGAAATGTGATATTGC+AGG + chr8_3:11624127-11624146 Msa1276770:intron 40.0%
AGGGATGCTTCAGAACTAAA+AGG + chr8_3:11624164-11624183 Msa1276770:CDS 40.0%
GGGATGCTTCAGAACTAAAA+GGG + chr8_3:11624165-11624184 Msa1276770:CDS 40.0%
AAGAATGGAGTTCCTTGACT+TGG + chr8_3:11624199-11624218 Msa1276770:CDS 40.0%
CTCATCCGCAAATATTACAG+CGG + chr8_3:11624247-11624266 Msa1276770:three_prime_UTR 40.0%
AAGAGCCGCTGTAATATTTG+CGG - chr8_3:11624255-11624274 None:intergenic 40.0%
ATAGGTTGTGGCAATGCCAT+TGG + chr8_3:11621440-11621459 Msa1276770:five_prime_UTR 45.0%
GTAGAAAGGTAGAGATCCTC+AGG - chr8_3:11621511-11621530 None:intergenic 45.0%
GGTTACAAAAGCCATCTCTC+AGG - chr8_3:11621885-11621904 None:intergenic 45.0%
ACGAGATGGTAGAAAGCTCA+TGG + chr8_3:11622240-11622259 Msa1276770:CDS 45.0%
CGAGATGGTAGAAAGCTCAT+GGG + chr8_3:11622241-11622260 Msa1276770:CDS 45.0%
GAGATGGTAGAAAGCTCATG+GGG + chr8_3:11622242-11622261 Msa1276770:CDS 45.0%
ACCAACAATTACCCTCTCAC+AGG - chr8_3:11622392-11622411 None:intergenic 45.0%
TAGGAACACGCGTCATATGT+GGG - chr8_3:11622594-11622613 None:intergenic 45.0%
ACCCTCCTGATTTCTTCTGT+AGG - chr8_3:11622613-11622632 None:intergenic 45.0%
!! ATTACGATGATGACGGGTGT+TGG + chr8_3:11623063-11623082 Msa1276770:intron 45.0%
CAGTGGATCCAAGGTAAAGT+CGG - chr8_3:11623160-11623179 None:intergenic 45.0%
AAACTCTTGCAGTGGATCCA+AGG - chr8_3:11623169-11623188 None:intergenic 45.0%
CCATACTAGTACAGGCAACT+TGG - chr8_3:11623199-11623218 None:intergenic 45.0%
CCAAGTTGCCTGTACTAGTA+TGG + chr8_3:11623196-11623215 Msa1276770:intron 45.0%
CTTTCACTCTCTGATTCACC+AGG + chr8_3:11623616-11623635 Msa1276770:intron 45.0%
ACTGCTAAAGCAAGTAACCC+TGG - chr8_3:11623637-11623656 None:intergenic 45.0%
GTGTCCATTGTACGCATGAT+TGG + chr8_3:11623939-11623958 Msa1276770:intron 45.0%
! GCATGATTGGTAAGCTCTAG+TGG + chr8_3:11623952-11623971 Msa1276770:intron 45.0%
GATATTGCAGGCACAGAAAG+TGG + chr8_3:11624139-11624158 Msa1276770:intron 45.0%
GGGAGGGATGAAAACATTGT+TGG + chr8_3:11624322-11624341 Msa1276770:three_prime_UTR 45.0%
TGCAGCTAACGCTGTTCTTG+AGG + chr8_3:11622363-11622382 Msa1276770:intron 50.0%
GCAGCTAACGCTGTTCTTGA+GGG + chr8_3:11622364-11622383 Msa1276770:intron 50.0%
GCCTGTGAGAGGGTAATTGT+TGG + chr8_3:11622388-11622407 Msa1276770:CDS 50.0%
!! TTTTCAGGATGTGGAGAGGG+AGG + chr8_3:11622561-11622580 Msa1276770:intron 50.0%
AGGAACACGCGTCATATGTG+GGG - chr8_3:11622593-11622612 None:intergenic 50.0%
GTAGGAACACGCGTCATATG+TGG - chr8_3:11622595-11622614 None:intergenic 50.0%
!! TGGTGGCTTGGTGCAATAGT+TGG + chr8_3:11623083-11623102 Msa1276770:intron 50.0%
GTGCCGTAAAACTCTTGCAG+TGG - chr8_3:11623177-11623196 None:intergenic 50.0%
GCTCGTTGCCATACTAGTAC+AGG - chr8_3:11623207-11623226 None:intergenic 50.0%
TGCAGGCACAGAAAGTGGAA+AGG + chr8_3:11624144-11624163 Msa1276770:intron 50.0%
GCAGGCACAGAAAGTGGAAA+GGG + chr8_3:11624145-11624164 Msa1276770:intron 50.0%
TATTTGCGGATGAGCGAGTG+TGG - chr8_3:11624241-11624260 None:intergenic 50.0%
TATGTCTTGGGCTGCTCCTG+AGG + chr8_3:11621492-11621511 Msa1276770:exon 55.0%
!! TTCTTGAGGGTGCCTGTGAG+AGG + chr8_3:11622377-11622396 Msa1276770:CDS 55.0%
!! TCTTGAGGGTGCCTGTGAGA+GGG + chr8_3:11622378-11622397 Msa1276770:CDS 55.0%
GGAACACGCGTCATATGTGG+GGG - chr8_3:11622592-11622611 None:intergenic 55.0%
!! ACGATGATGACGGGTGTTGG+TGG + chr8_3:11623066-11623085 Msa1276770:intron 55.0%
!! GATGACGGGTGTTGGTGGCT+TGG + chr8_3:11623071-11623090 Msa1276770:intron 60.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 11621243 11624498 11621243 ID=Msa1276770;Name=Msa1276770
chr8_3 mRNA 11621243 11624498 11621243 ID=Msa1276770-mRNA-1;Parent=Msa1276770;Name=Msa1276770-mRNA-1;_AED=0.14;_eAED=0.14;_QI=250|1|1|1|1|1|5|272|128
chr8_3 exon 11621243 11621553 11621243 ID=Msa1276770-mRNA-1:exon:4709;Parent=Msa1276770-mRNA-1
chr8_3 exon 11622221 11622289 11622221 ID=Msa1276770-mRNA-1:exon:4710;Parent=Msa1276770-mRNA-1
chr8_3 exon 11622368 11622483 11622368 ID=Msa1276770-mRNA-1:exon:4711;Parent=Msa1276770-mRNA-1
chr8_3 exon 11622568 11622630 11622568 ID=Msa1276770-mRNA-1:exon:4712;Parent=Msa1276770-mRNA-1
chr8_3 exon 11624149 11624498 11624149 ID=Msa1276770-mRNA-1:exon:4713;Parent=Msa1276770-mRNA-1
chr8_3 five_prime_UTR 11621243 11621492 11621243 ID=Msa1276770-mRNA-1:five_prime_utr;Parent=Msa1276770-mRNA-1
chr8_3 CDS 11621493 11621553 11621493 ID=Msa1276770-mRNA-1:cds;Parent=Msa1276770-mRNA-1
chr8_3 CDS 11622221 11622289 11622221 ID=Msa1276770-mRNA-1:cds;Parent=Msa1276770-mRNA-1
chr8_3 CDS 11622368 11622483 11622368 ID=Msa1276770-mRNA-1:cds;Parent=Msa1276770-mRNA-1
chr8_3 CDS 11622568 11622630 11622568 ID=Msa1276770-mRNA-1:cds;Parent=Msa1276770-mRNA-1
chr8_3 CDS 11624149 11624226 11624149 ID=Msa1276770-mRNA-1:cds;Parent=Msa1276770-mRNA-1
chr8_3 three_prime_UTR 11624227 11624498 11624227 ID=Msa1276770-mRNA-1:three_prime_utr;Parent=Msa1276770-mRNA-1
Gene Sequence

>Msa1276770

ATGTCTTGGGCTGCTCCTGAGGATCTCTACCTTTCTACTTCACTTGCTAGTTATCTTGACAAGAAACTTCTTATATTGCTACGAGATGGTAGAAAGCTCATGGGGACACTTCGCTCTTTTGATCAATTTGCTAACGCTGTTCTTGAGGGTGCCTGTGAGAGGGTAATTGTTGGTGATCTATATTGTGACATCCCTCTAGGTCTTTATGTAATCCGTGGGGAAAATGTTGTTCTAATTGGTGAGATGGATGTGGAGAGGGAGGAACTTCCCCCACATATGACGCGTGTTCCTACAGAAGAAATCAGGAGGGCACAGAAAGTGGAAAGGGATGCTTCAGAACTAAAAGGGACTATGAGAAAAAGAATGGAGTTCCTTGACTTGGACTAG
Protein sequence

>Msa1276770

MSWAAPEDLYLSTSLASYLDKKLLILLRDGRKLMGTLRSFDQFANAVLEGACERVIVGDLYCDIPLGLYVIRGENVVLIGEMDVEREELPPHMTRVPTEEIRRAQKVERDASELKGTMRKRMEFLDLD*