AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1234880

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1234880 MtrunA17_Chr8g0344441 100.000 128 0 0 1 128 1 128 3.20e-91 259
Msa1234880 MtrunA17_Chr2g0301051 82.812 128 22 0 1 128 1 128 2.35e-76 222
Msa1234880 MtrunA17_Chr4g0044211 38.356 73 44 1 14 85 7 79 1.97e-12 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1234880 AT3G14080.2 86.719 128 17 0 1 128 1 128 1.72e-80 233
Msa1234880 AT3G14080.1 86.719 128 17 0 1 128 1 128 1.72e-80 233
Msa1234880 AT1G19120.1 81.250 128 24 0 1 128 1 128 2.07e-74 217
Msa1234880 AT3G14080.4 83.621 116 17 1 15 128 3 118 1.43e-65 194
Msa1234880 AT3G14080.3 90.123 81 8 0 1 81 1 81 5.61e-50 154
Msa1234880 AT1G65700.2 38.667 75 45 1 12 85 4 78 5.58e-12 58.2
Msa1234880 AT1G65700.1 38.667 75 45 1 12 85 4 78 5.58e-12 58.2

Find 62 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGTTAATTTCTGGAAATTT+AGG 0.147955 8_2:-12625147 Msa1234880:five_prime_UTR
GAAGCGAAAGAGTGAAAATT+TGG 0.161483 8_2:+12625249 None:intergenic
TAGATCGAAATCACAATAAA+AGG 0.191374 8_2:+12625190 None:intergenic
AAGTGGGTGATTTGAGAAAA+AGG 0.224442 8_2:+12625216 None:intergenic
TCTTCTTTATATATAGGAAT+TGG 0.262226 8_2:-12625116 Msa1234880:five_prime_UTR
AGGGATGCTTCAGAACTAAA+AGG 0.302591 8_2:-12622338 Msa1234880:CDS
GGGGAAAATGTTGTTCTAAT+TGG 0.311566 8_2:-12624062 Msa1234880:CDS
AAGCGAAAGAGTGAAAATTT+GGG 0.332275 8_2:+12625250 None:intergenic
CCTAAATTTCCAGAAATTAA+CGG 0.358389 8_2:+12625147 None:intergenic
CTTCTTTATATATAGGAATT+GGG 0.377863 8_2:-12625115 Msa1234880:five_prime_UTR
TTGTTCTTCTGTGATATTGT+TGG 0.380565 8_2:-12622210 Msa1234880:three_prime_UTR
GGAATTCAATCTCAGTGAAT+AGG 0.381686 8_2:-12625094 Msa1234880:five_prime_UTR
AGAAAGCAACGGAATAATAA+TGG 0.398474 8_2:+12622136 None:intergenic
ATCACAATAAAAGGGTTAAG+TGG 0.445738 8_2:+12625199 None:intergenic
GTAGAAAGGTAGAGATCCTC+AGG 0.460456 8_2:+12625008 None:intergenic
TATCTGGTTTGGAAATGAAA+AGG 0.463066 8_2:-12622040 Msa1234880:three_prime_UTR
GATATTCAAAATGTCAGTTA+TGG 0.463126 8_2:+12622059 None:intergenic
TAACGGAATGAAAAGAACTA+AGG 0.464650 8_2:+12625164 None:intergenic
AGATCGAAATCACAATAAAA+GGG 0.480054 8_2:+12625191 None:intergenic
AAGAATGGAGTTCCTTGACT+TGG 0.481713 8_2:-12622303 Msa1234880:CDS
GCAGCTAACGCTGTTCTTGA+GGG 0.491345 8_2:-12624152 Msa1234880:intron
GTGTTCCTACAGAAGAAATC+AGG 0.501365 8_2:-12623911 Msa1234880:CDS
TTCTTGAGGGTGCCTGTGAG+AGG 0.503890 8_2:-12624139 Msa1234880:CDS
GTAGGAACACGCGTCATATG+TGG 0.510896 8_2:+12623924 None:intergenic
TGCAGCTAACGCTGTTCTTG+AGG 0.511473 8_2:-12624153 Msa1234880:intron
TATGTCTTGGGCTGCTCCTG+AGG 0.516307 8_2:-12625024 Msa1234880:CDS
GATATTTCAGGCACAGAAAG+TGG 0.522168 8_2:-12622363 Msa1234880:intron
AACTAGCAAGTGAAGTAGAA+AGG 0.522695 8_2:+12624994 None:intergenic
CTATATTGTGACATCCCTCT+AGG 0.524354 8_2:-12624101 Msa1234880:CDS
ACGAGATGGTAGAAAGCTCA+TGG 0.524959 8_2:-12624276 Msa1234880:CDS
CAATCTCAGTGAATAGGTTG+TGG 0.530649 8_2:-12625088 Msa1234880:five_prime_UTR
AAGAACAAAATAAAATAACT+TGG 0.534155 8_2:+12622225 None:intergenic
GGGATGCTTCAGAACTAAAA+GGG 0.534576 8_2:-12622337 Msa1234880:CDS
TAGGAACACGCGTCATATGT+GGG 0.543008 8_2:+12623925 None:intergenic
TCAGGCACAGAAAGTGGAAA+GGG 0.543526 8_2:-12622357 Msa1234880:intron
AAGAGCCGCTGTAATATTTG+CGG 0.553134 8_2:+12622250 None:intergenic
TCACAATAAAAGGGTTAAGT+GGG 0.553685 8_2:+12625200 None:intergenic
GCCTGTGAGAGGGTAATTGT+TGG 0.562739 8_2:-12624128 Msa1234880:CDS
AGCGAAAGAGTGAAAATTTG+GGG 0.565663 8_2:+12625251 None:intergenic
CTAGGTCTTTATGTAATCCG+TGG 0.570170 8_2:-12624083 Msa1234880:CDS
TGTTGTTCTAATTGGTGAGA+TGG 0.571580 8_2:-12624054 Msa1234880:intron
CTTCTTATATTGCTACGAGA+TGG 0.573728 8_2:-12624290 Msa1234880:CDS
TATATCACAAAATTCACCAA+TGG 0.584239 8_2:+12625060 None:intergenic
CGAGATGGTAGAAAGCTCAT+GGG 0.590247 8_2:-12624275 Msa1234880:CDS
TAGGTCTTTATGTAATCCGT+GGG 0.590765 8_2:-12624082 Msa1234880:CDS
TTCAGGCACAGAAAGTGGAA+AGG 0.592068 8_2:-12622358 Msa1234880:intron
TCTTGAGGGTGCCTGTGAGA+GGG 0.598880 8_2:-12624138 Msa1234880:CDS
TTGACAAGAAAAGAAAGCAA+CGG 0.605836 8_2:+12622125 None:intergenic
ACCCTCCTGATTTCTTCTGT+AGG 0.607048 8_2:+12623906 None:intergenic
ACCAACAATTACCCTCTCAC+AGG 0.608128 8_2:+12624127 None:intergenic
ATAGGTTGTGGCAATGCCAT+TGG 0.610982 8_2:-12625076 Msa1234880:five_prime_UTR
TATTTGCGGATGAGCGAGTG+TGG 0.613060 8_2:+12622264 None:intergenic
AGGAACACGCGTCATATGTG+GGG 0.634207 8_2:+12623926 None:intergenic
TTCCTACAGAAGAAATCAGG+AGG 0.637433 8_2:-12623908 Msa1234880:CDS
TTAATCACTAGTCCAAGTCA+AGG 0.651795 8_2:+12622291 None:intergenic
CTCATCCGCAAATATTACAG+CGG 0.668106 8_2:-12622255 Msa1234880:three_prime_UTR
GGATTACATAAAGACCTAGA+GGG 0.677541 8_2:+12624087 None:intergenic
GGAACACGCGTCATATGTGG+GGG 0.684221 8_2:+12623927 None:intergenic
CGGATTACATAAAGACCTAG+AGG 0.700189 8_2:+12624086 None:intergenic
TCCTACAGAAGAAATCAGGA+GGG 0.714873 8_2:-12623907 Msa1234880:intron
GAGATGGTAGAAAGCTCATG+GGG 0.721011 8_2:-12624274 Msa1234880:CDS
AGGTCTTTATGTAATCCGTG+GGG 0.758020 8_2:-12624081 Msa1234880:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTCTTCTTTATATAT+AGG - chr8_2:12622176-12622195 Msa1234880:three_prime_UTR 10.0%
!! AAAAATCATAATTATGTCTT+GGG - chr8_2:12622262-12622281 Msa1234880:three_prime_UTR 15.0%
!! ATTAGACAAAAATAAAAACA+TGG + chr8_2:12622369-12622388 None:intergenic 15.0%
!!! ATCATAAAGAAGTTTAATTT+AGG + chr8_2:12623425-12623444 None:intergenic 15.0%
!! TATTAATATACGACAATAAT+AGG + chr8_2:12623533-12623552 None:intergenic 15.0%
!!! TAAGATTTTATTTTTTAAGC+AGG + chr8_2:12624675-12624694 None:intergenic 15.0%
!!! TTGATATTTTTGTTATGATT+TGG - chr8_2:12624817-12624836 Msa1234880:intron 15.0%
!! AAGAACAAAATAAAATAACT+TGG + chr8_2:12625076-12625095 None:intergenic 15.0%
!! TCTTCTTTATATATAGGAAT+TGG - chr8_2:12622182-12622201 Msa1234880:three_prime_UTR 20.0%
!! CTTCTTTATATATAGGAATT+GGG - chr8_2:12622183-12622202 Msa1234880:three_prime_UTR 20.0%
!! GAAAAATCATAATTATGTCT+TGG - chr8_2:12622261-12622280 Msa1234880:three_prime_UTR 20.0%
!! AAGAAACATATAGATATGTT+TGG - chr8_2:12622618-12622637 Msa1234880:intron 20.0%
!! TTATTAATGGTAATTAAGCT+TGG - chr8_2:12622908-12622927 Msa1234880:intron 20.0%
!! TATTAATGGTAATTAAGCTT+GGG - chr8_2:12622909-12622928 Msa1234880:intron 20.0%
!!! GAAATAGTTTTAAATGCAAA+GGG + chr8_2:12623288-12623307 None:intergenic 20.0%
!!! AGAAATAGTTTTAAATGCAA+AGG + chr8_2:12623289-12623308 None:intergenic 20.0%
!!! AAAAGTACAATAATTGTGTT+TGG + chr8_2:12623758-12623777 None:intergenic 20.0%
!! AAATAAAGTGGATAAACTAA+TGG - chr8_2:12624014-12624033 Msa1234880:intron 20.0%
!!! TTCAAGGATATATCAAATTT+TGG + chr8_2:12624631-12624650 None:intergenic 20.0%
!!! TGAAATTTAGGATTAATTCA+AGG + chr8_2:12624647-12624666 None:intergenic 20.0%
!! TTGAATTAATCCTAAATTTC+AGG - chr8_2:12624646-12624665 Msa1234880:intron 20.0%
!! ATCATAACAAAAATATCAAC+TGG + chr8_2:12624816-12624835 None:intergenic 20.0%
!! GTTAAATATTCTATATTGAG+TGG - chr8_2:12624906-12624925 Msa1234880:intron 20.0%
!!! AAATTTGGGGATTTTGAAAT+GGG + chr8_2:12622037-12622056 None:intergenic 25.0%
!!! AAAATTTGGGGATTTTGAAA+TGG + chr8_2:12622038-12622057 None:intergenic 25.0%
! AGATCGAAATCACAATAAAA+GGG + chr8_2:12622110-12622129 None:intergenic 25.0%
! TAGATCGAAATCACAATAAA+AGG + chr8_2:12622111-12622130 None:intergenic 25.0%
! CCTAAATTTCCAGAAATTAA+CGG + chr8_2:12622154-12622173 None:intergenic 25.0%
! TATATCACAAAATTCACCAA+TGG + chr8_2:12622241-12622260 None:intergenic 25.0%
! ATAATTAAAAAAGACCCGTT+TGG - chr8_2:12622425-12622444 Msa1234880:intron 25.0%
! ATAAATAAGTCAATCCAAAC+AGG + chr8_2:12622495-12622514 None:intergenic 25.0%
! TATCTATATGTTTCTTGTCA+AGG + chr8_2:12622614-12622633 None:intergenic 25.0%
! TGATGAAAACCAGTTATTAA+TGG - chr8_2:12622895-12622914 Msa1234880:intron 25.0%
! GCTTAATTACCATTAATAAC+TGG + chr8_2:12622907-12622926 None:intergenic 25.0%
! TCAGTAATAGAACATAAGAT+TGG + chr8_2:12622971-12622990 None:intergenic 25.0%
! TAGTCAGTTAGAAATTAGAT+TGG - chr8_2:12623647-12623666 Msa1234880:intron 25.0%
!! AAATTAGATTGGTCATGTTT+GGG - chr8_2:12623658-12623677 Msa1234880:intron 25.0%
! GTGATATCTACTAATTGTTA+TGG - chr8_2:12623782-12623801 Msa1234880:intron 25.0%
!! TGCATACTTCATAATCATTT+TGG - chr8_2:12624037-12624056 Msa1234880:intron 25.0%
! GAGAGAATATATAGCTAATT+AGG - chr8_2:12624197-12624216 Msa1234880:intron 25.0%
! TAACTAATTAGCTACAACTA+AGG + chr8_2:12624312-12624331 None:intergenic 25.0%
! TTGGTCAATTTATACATTGA+AGG - chr8_2:12624498-12624517 Msa1234880:intron 25.0%
! TTATGTATTACCACTGTAAA+TGG + chr8_2:12624561-12624580 None:intergenic 25.0%
! GATATTCAAAATGTCAGTTA+TGG + chr8_2:12625242-12625261 None:intergenic 25.0%
!! TAACTGACATTTTGAATATC+TGG - chr8_2:12625242-12625261 Msa1234880:five_prime_UTR 25.0%
AAGCGAAAGAGTGAAAATTT+GGG + chr8_2:12622051-12622070 None:intergenic 30.0%
TCACAATAAAAGGGTTAAGT+GGG + chr8_2:12622101-12622120 None:intergenic 30.0%
ATCACAATAAAAGGGTTAAG+TGG + chr8_2:12622102-12622121 None:intergenic 30.0%
TAACGGAATGAAAAGAACTA+AGG + chr8_2:12622137-12622156 None:intergenic 30.0%
! CTTTTCATTCCGTTAATTTC+TGG - chr8_2:12622142-12622161 Msa1234880:three_prime_UTR 30.0%
! CCGTTAATTTCTGGAAATTT+AGG - chr8_2:12622151-12622170 Msa1234880:three_prime_UTR 30.0%
CAGCTTATAAGCTATAATCA+AGG + chr8_2:12622403-12622422 None:intergenic 30.0%
CTTGATTATAGCTTATAAGC+TGG - chr8_2:12622401-12622420 Msa1234880:intron 30.0%
AATAAGTTCGTGTTACCAAA+CGG + chr8_2:12622443-12622462 None:intergenic 30.0%
TTTCTTGTCAAGGTAGTTTA+CGG + chr8_2:12622604-12622623 None:intergenic 30.0%
TACCTATCAACTTAAGCTTT+GGG - chr8_2:12622697-12622716 Msa1234880:intron 30.0%
ACATCCTGAAAATTGAAAGA+CGG + chr8_2:12623335-12623354 None:intergenic 30.0%
CATTGTTTGTAAAGAGAAGT+AGG + chr8_2:12623561-12623580 None:intergenic 30.0%
CTACTTCTCTTTACAAACAA+TGG - chr8_2:12623559-12623578 Msa1234880:intron 30.0%
! GAAATTAGATTGGTCATGTT+TGG - chr8_2:12623657-12623676 Msa1234880:intron 30.0%
ATTGTTATGGTAAGTTACCT+TGG - chr8_2:12623795-12623814 Msa1234880:intron 30.0%
TACGATTATTACGATGATGA+TGG - chr8_2:12623840-12623859 Msa1234880:intron 30.0%
ACGATTATTACGATGATGAT+GGG - chr8_2:12623841-12623860 Msa1234880:intron 30.0%
TTAAGCTAGTACGACTTATT+AGG - chr8_2:12624115-12624134 Msa1234880:CDS 30.0%
TAAGCTAGTACGACTTATTA+GGG - chr8_2:12624116-12624135 Msa1234880:CDS 30.0%
! AGATGTTTTCTTTGTGTAGT+TGG - chr8_2:12624153-12624172 Msa1234880:intron 30.0%
ACTTGAGAATATAGCTTAGT+TGG - chr8_2:12624236-12624255 Msa1234880:intron 30.0%
TACTATATTGAAATGCTTCG+TGG - chr8_2:12624466-12624485 Msa1234880:intron 30.0%
TATAACTCAACCATTTACAG+TGG - chr8_2:12624548-12624567 Msa1234880:intron 30.0%
GATATGTTAAAGAGGTCATT+TGG + chr8_2:12624589-12624608 None:intergenic 30.0%
GCAATGTAGATATGTTAAAG+AGG + chr8_2:12624597-12624616 None:intergenic 30.0%
TACAATGGACAAGTAGTTTA+TGG + chr8_2:12624707-12624726 None:intergenic 30.0%
TTGTCCATTGTACATATGAT+TGG - chr8_2:12624715-12624734 Msa1234880:intron 30.0%
CTTACCAATCATATGTACAA+TGG + chr8_2:12624722-12624741 None:intergenic 30.0%
TTGTTCTTCTGTGATATTGT+TGG - chr8_2:12625088-12625107 Msa1234880:five_prime_UTR 30.0%
!!! TGTGATATTGTTGGTTTTCA+TGG - chr8_2:12625097-12625116 Msa1234880:five_prime_UTR 30.0%
!!! GTGATATTGTTGGTTTTCAT+GGG - chr8_2:12625098-12625117 Msa1234880:five_prime_UTR 30.0%
AGAAAGCAACGGAATAATAA+TGG + chr8_2:12625165-12625184 None:intergenic 30.0%
TTGACAAGAAAAGAAAGCAA+CGG + chr8_2:12625176-12625195 None:intergenic 30.0%
! TGCTTTCTTTTCTTGTCAAA+AGG - chr8_2:12625177-12625196 Msa1234880:five_prime_UTR 30.0%
! GACATTTTGAATATCTGGTT+TGG - chr8_2:12625247-12625266 Msa1234880:five_prime_UTR 30.0%
!! TATCTGGTTTGGAAATGAAA+AGG - chr8_2:12625258-12625277 Msa1234880:five_prime_UTR 30.0%
AGCGAAAGAGTGAAAATTTG+GGG + chr8_2:12622050-12622069 None:intergenic 35.0%
GAAGCGAAAGAGTGAAAATT+TGG + chr8_2:12622052-12622071 None:intergenic 35.0%
AGTGGGTGATTTGAGAAAAA+GGG + chr8_2:12622084-12622103 None:intergenic 35.0%
AAGTGGGTGATTTGAGAAAA+AGG + chr8_2:12622085-12622104 None:intergenic 35.0%
GGAATTCAATCTCAGTGAAT+AGG - chr8_2:12622204-12622223 Msa1234880:three_prime_UTR 35.0%
AACTAGCAAGTGAAGTAGAA+AGG + chr8_2:12622307-12622326 None:intergenic 35.0%
ATAAGTTCGTGTTACCAAAC+GGG + chr8_2:12622442-12622461 None:intergenic 35.0%
AAGTAGCTTATTGACCTGTT+TGG - chr8_2:12622478-12622497 Msa1234880:intron 35.0%
TTCTTGTCAAGGTAGTTTAC+GGG + chr8_2:12622603-12622622 None:intergenic 35.0%
CTACCTATCAACTTAAGCTT+TGG - chr8_2:12622696-12622715 Msa1234880:intron 35.0%
ACCTATCAACTTAAGCTTTG+GGG - chr8_2:12622698-12622717 Msa1234880:intron 35.0%
CTTCTTATATTGCTACGAGA+TGG - chr8_2:12623008-12623027 Msa1234880:intron 35.0%
! CTTCGCTCTTTTGATCAATT+TGG - chr8_2:12623050-12623069 Msa1234880:intron 35.0%
GTAAAGATTTGACATGCGTT+AGG - chr8_2:12623092-12623111 Msa1234880:intron 35.0%
GTTAGCTGCATGTTATTGAT+AGG + chr8_2:12623135-12623154 None:intergenic 35.0%
GGATTACATAAAGACCTAGA+GGG + chr8_2:12623214-12623233 None:intergenic 35.0%
TAGGTCTTTATGTAATCCGT+GGG - chr8_2:12623216-12623235 Msa1234880:intron 35.0%
! TTAGAACAACATTTTCCCCA+CGG + chr8_2:12623235-12623254 None:intergenic 35.0%
GGGGAAAATGTTGTTCTAAT+TGG - chr8_2:12623236-12623255 Msa1234880:intron 35.0%
TGTTGTTCTAATTGGTGAGA+TGG - chr8_2:12623244-12623263 Msa1234880:intron 35.0%
! ACAACCGTCTTTCAATTTTC+AGG - chr8_2:12623328-12623347 Msa1234880:intron 35.0%
!! GTCTTTCAATTTTCAGGATG+TGG - chr8_2:12623334-12623353 Msa1234880:intron 35.0%
CATTGTATTGCCTTAAGTGA+GGG + chr8_2:12623481-12623500 None:intergenic 35.0%
ACATTGTATTGCCTTAAGTG+AGG + chr8_2:12623482-12623501 None:intergenic 35.0%
TCTACATGAAACAGATTCGT+AGG - chr8_2:12623615-12623634 Msa1234880:intron 35.0%
CTACATGAAACAGATTCGTA+GGG - chr8_2:12623616-12623635 Msa1234880:intron 35.0%
CAGAGTTAGAGTTACAGAAT+TGG - chr8_2:12623687-12623706 Msa1234880:intron 35.0%
! GTTACAGAATTGGCTTAGAT+AGG - chr8_2:12623697-12623716 Msa1234880:intron 35.0%
TAGGAGGTTATCTCATAACT+CGG - chr8_2:12623716-12623735 Msa1234880:intron 35.0%
!! TTACCTTGGCTTGTGAAATA+AGG - chr8_2:12623809-12623828 Msa1234880:intron 35.0%
!! TACCTTGGCTTGTGAAATAA+GGG - chr8_2:12623810-12623829 Msa1234880:intron 35.0%
! GATATAGTGTGGGTTACTTT+AGG + chr8_2:12623922-12623941 None:intergenic 35.0%
CTAGCTTAATCGCTAGAATT+TGG + chr8_2:12624104-12624123 None:intergenic 35.0%
AATTAGCTACAACTAAGGAC+AGG + chr8_2:12624307-12624326 None:intergenic 35.0%
! TGCTTCGTGGTTATGATTTT+TGG - chr8_2:12624479-12624498 Msa1234880:intron 35.0%
AAGCAGGATTCCTGAAATTT+AGG + chr8_2:12624659-12624678 None:intergenic 35.0%
! ATATGATTGGTAAGCTCTAG+TGG - chr8_2:12624728-12624747 Msa1234880:intron 35.0%
TGTATGACTGCATGTCAAAA+CGG + chr8_2:12624860-12624879 None:intergenic 35.0%
GAGTGGAAATGTGATATTTC+AGG - chr8_2:12624923-12624942 Msa1234880:intron 35.0%
AGGGACTATGAGAAAAAGAA+TGG - chr8_2:12624980-12624999 Msa1234880:intron 35.0%
TTAATCACTAGTCCAAGTCA+AGG + chr8_2:12625010-12625029 None:intergenic 35.0%
!!! ATATTGTTGGTTTTCATGGG+AGG - chr8_2:12625101-12625120 Msa1234880:five_prime_UTR 35.0%
!!! TATTGTTGGTTTTCATGGGA+GGG - chr8_2:12625102-12625121 Msa1234880:five_prime_UTR 35.0%
CAATCTCAGTGAATAGGTTG+TGG - chr8_2:12622210-12622229 Msa1234880:three_prime_UTR 40.0%
ATGATCTAACTCCTGAGAGA+TGG - chr8_2:12622653-12622672 Msa1234880:intron 40.0%
! GTTGATAGGTAGCACATGAA+TGG + chr8_2:12622688-12622707 None:intergenic 40.0%
!! ACCCCAAAGCTTAAGTTGAT+AGG + chr8_2:12622702-12622721 None:intergenic 40.0%
ATCTTGCACACAAGTCTTTC+TGG - chr8_2:12622838-12622857 Msa1234880:intron 40.0%
CTATATTGTGACATCCCTCT+AGG - chr8_2:12623197-12623216 Msa1234880:intron 40.0%
CGGATTACATAAAGACCTAG+AGG + chr8_2:12623215-12623234 None:intergenic 40.0%
CTAGGTCTTTATGTAATCCG+TGG - chr8_2:12623215-12623234 Msa1234880:intron 40.0%
AGGTCTTTATGTAATCCGTG+GGG - chr8_2:12623217-12623236 Msa1234880:intron 40.0%
!! TCAATTTTCAGGATGTGGAG+AGG - chr8_2:12623339-12623358 Msa1234880:intron 40.0%
!! CAATTTTCAGGATGTGGAGA+GGG - chr8_2:12623340-12623359 Msa1234880:intron 40.0%
GTGTTCCTACAGAAGAAATC+AGG - chr8_2:12623387-12623406 Msa1234880:intron 40.0%
TTCCTACAGAAGAAATCAGG+AGG - chr8_2:12623390-12623409 Msa1234880:intron 40.0%
TCCTACAGAAGAAATCAGGA+GGG - chr8_2:12623391-12623410 Msa1234880:intron 40.0%
ATAGGCACTTCTGCAATGAA+TGG + chr8_2:12623515-12623534 None:intergenic 40.0%
! ACAGAATTGGCTTAGATAGG+AGG - chr8_2:12623700-12623719 Msa1234880:intron 40.0%
! TACCCTTATTTCACAAGCCA+AGG + chr8_2:12623815-12623834 None:intergenic 40.0%
!! ATTACGATGATGATGGGTGT+TGG - chr8_2:12623847-12623866 Msa1234880:intron 40.0%
GGTAAAGTCGGATATAGTGT+GGG + chr8_2:12623932-12623951 None:intergenic 40.0%
AGGTAAAGTCGGATATAGTG+TGG + chr8_2:12623933-12623952 None:intergenic 40.0%
CACTATATCCGACTTTACCT+TGG - chr8_2:12623933-12623952 Msa1234880:CDS 40.0%
GCAACGAGCTAGAAATAAAG+TGG - chr8_2:12624002-12624021 Msa1234880:intron 40.0%
AGTACGACTTATTAGGGATG+CGG - chr8_2:12624122-12624141 Msa1234880:CDS 40.0%
TTTCACTCTCTGATTCACCA+GGG - chr8_2:12624404-12624423 Msa1234880:intron 40.0%
GATATTTCAGGCACAGAAAG+TGG - chr8_2:12624935-12624954 Msa1234880:intron 40.0%
AGGGATGCTTCAGAACTAAA+AGG - chr8_2:12624960-12624979 Msa1234880:intron 40.0%
GGGATGCTTCAGAACTAAAA+GGG - chr8_2:12624961-12624980 Msa1234880:intron 40.0%
AAGAATGGAGTTCCTTGACT+TGG - chr8_2:12624995-12625014 Msa1234880:CDS 40.0%
CTCATCCGCAAATATTACAG+CGG - chr8_2:12625043-12625062 Msa1234880:exon 40.0%
AAGAGCCGCTGTAATATTTG+CGG + chr8_2:12625051-12625070 None:intergenic 40.0%
ATAGGTTGTGGCAATGCCAT+TGG - chr8_2:12622222-12622241 Msa1234880:three_prime_UTR 45.0%
GTAGAAAGGTAGAGATCCTC+AGG + chr8_2:12622293-12622312 None:intergenic 45.0%
GGTTACAAAAGCCATCTCTC+AGG + chr8_2:12622667-12622686 None:intergenic 45.0%
ACGAGATGGTAGAAAGCTCA+TGG - chr8_2:12623022-12623041 Msa1234880:intron 45.0%
CGAGATGGTAGAAAGCTCAT+GGG - chr8_2:12623023-12623042 Msa1234880:intron 45.0%
GAGATGGTAGAAAGCTCATG+GGG - chr8_2:12623024-12623043 Msa1234880:intron 45.0%
ACCAACAATTACCCTCTCAC+AGG + chr8_2:12623174-12623193 None:intergenic 45.0%
TAGGAACACGCGTCATATGT+GGG + chr8_2:12623376-12623395 None:intergenic 45.0%
ACCCTCCTGATTTCTTCTGT+AGG + chr8_2:12623395-12623414 None:intergenic 45.0%
CTTAAGTGAGGGCAAGAACA+AGG + chr8_2:12623470-12623489 None:intergenic 45.0%
CTTGTTCTTGCCCTCACTTA+AGG - chr8_2:12623468-12623487 Msa1234880:intron 45.0%
ACTTCTGCAATGAATGGCCA+CGG + chr8_2:12623509-12623528 None:intergenic 45.0%
CAGTGGATCCAAGGTAAAGT+CGG + chr8_2:12623944-12623963 None:intergenic 45.0%
AAACTCTTGCAGTGGATCCA+AGG + chr8_2:12623953-12623972 None:intergenic 45.0%
GTGCTGTAAAACTCTTGCAG+TGG + chr8_2:12623961-12623980 None:intergenic 45.0%
CCATACTAGTACAGGCAACT+TGG + chr8_2:12623983-12624002 None:intergenic 45.0%
CCAAGTTGCCTGTACTAGTA+TGG - chr8_2:12623980-12623999 Msa1234880:intron 45.0%
CTTTCACTCTCTGATTCACC+AGG - chr8_2:12624403-12624422 Msa1234880:intron 45.0%
ACTGCTAAAGCAAGTATCCC+TGG + chr8_2:12624424-12624443 None:intergenic 45.0%
TTCAGGCACAGAAAGTGGAA+AGG - chr8_2:12624940-12624959 Msa1234880:intron 45.0%
TCAGGCACAGAAAGTGGAAA+GGG - chr8_2:12624941-12624960 Msa1234880:intron 45.0%
TGCAGCTAACGCTGTTCTTG+AGG - chr8_2:12623145-12623164 Msa1234880:intron 50.0%
GCAGCTAACGCTGTTCTTGA+GGG - chr8_2:12623146-12623165 Msa1234880:intron 50.0%
GCCTGTGAGAGGGTAATTGT+TGG - chr8_2:12623170-12623189 Msa1234880:intron 50.0%
!! TTTTCAGGATGTGGAGAGGG+AGG - chr8_2:12623343-12623362 Msa1234880:intron 50.0%
AGGAACACGCGTCATATGTG+GGG + chr8_2:12623375-12623394 None:intergenic 50.0%
GTAGGAACACGCGTCATATG+TGG + chr8_2:12623377-12623396 None:intergenic 50.0%
GGCAATACAATGTTGTGCCG+TGG - chr8_2:12623489-12623508 Msa1234880:intron 50.0%
!! ACGATGATGATGGGTGTTGG+TGG - chr8_2:12623850-12623869 Msa1234880:intron 50.0%
!! TGGTGGCTTGGTGCAATAGT+TGG - chr8_2:12623867-12623886 Msa1234880:intron 50.0%
GCTCGTTGCCATACTAGTAC+AGG + chr8_2:12623991-12624010 None:intergenic 50.0%
TATTTGCGGATGAGCGAGTG+TGG + chr8_2:12625037-12625056 None:intergenic 50.0%
TATGTCTTGGGCTGCTCCTG+AGG - chr8_2:12622274-12622293 Msa1234880:three_prime_UTR 55.0%
!! TTCTTGAGGGTGCCTGTGAG+AGG - chr8_2:12623159-12623178 Msa1234880:intron 55.0%
!! TCTTGAGGGTGCCTGTGAGA+GGG - chr8_2:12623160-12623179 Msa1234880:intron 55.0%
GGAACACGCGTCATATGTGG+GGG + chr8_2:12623374-12623393 None:intergenic 55.0%
!! GATGATGGGTGTTGGTGGCT+TGG - chr8_2:12623855-12623874 Msa1234880:intron 55.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 12622026 12625294 12622026 ID=Msa1234880;Name=Msa1234880
chr8_2 mRNA 12622026 12625294 12622026 ID=Msa1234880-mRNA-1;Parent=Msa1234880;Name=Msa1234880-mRNA-1;_AED=0.14;_eAED=0.14;_QI=249|1|1|1|1|1|5|272|128
chr8_2 exon 12622026 12622375 12622026 ID=Msa1234880-mRNA-1:exon:4921;Parent=Msa1234880-mRNA-1
chr8_2 exon 12623908 12623970 12623908 ID=Msa1234880-mRNA-1:exon:4920;Parent=Msa1234880-mRNA-1
chr8_2 exon 12624055 12624170 12624055 ID=Msa1234880-mRNA-1:exon:4919;Parent=Msa1234880-mRNA-1
chr8_2 exon 12624249 12624317 12624249 ID=Msa1234880-mRNA-1:exon:4918;Parent=Msa1234880-mRNA-1
chr8_2 exon 12624985 12625294 12624985 ID=Msa1234880-mRNA-1:exon:4917;Parent=Msa1234880-mRNA-1
chr8_2 five_prime_UTR 12625046 12625294 12625046 ID=Msa1234880-mRNA-1:five_prime_utr;Parent=Msa1234880-mRNA-1
chr8_2 CDS 12624985 12625045 12624985 ID=Msa1234880-mRNA-1:cds;Parent=Msa1234880-mRNA-1
chr8_2 CDS 12624249 12624317 12624249 ID=Msa1234880-mRNA-1:cds;Parent=Msa1234880-mRNA-1
chr8_2 CDS 12624055 12624170 12624055 ID=Msa1234880-mRNA-1:cds;Parent=Msa1234880-mRNA-1
chr8_2 CDS 12623908 12623970 12623908 ID=Msa1234880-mRNA-1:cds;Parent=Msa1234880-mRNA-1
chr8_2 CDS 12622298 12622375 12622298 ID=Msa1234880-mRNA-1:cds;Parent=Msa1234880-mRNA-1
chr8_2 three_prime_UTR 12622026 12622297 12622026 ID=Msa1234880-mRNA-1:three_prime_utr;Parent=Msa1234880-mRNA-1
Gene Sequence

>Msa1234880

ATGTCTTGGGCTGCTCCTGAGGATCTCTACCTTTCTACTTCACTTGCTAGTTATCTTGACAAGAAACTTCTTATATTGCTACGAGATGGTAGAAAGCTCATGGGGACACTTCGCTCTTTTGATCAATTTGCTAACGCTGTTCTTGAGGGTGCCTGTGAGAGGGTAATTGTTGGTGATCTATATTGTGACATCCCTCTAGGTCTTTATGTAATCCGTGGGGAAAATGTTGTTCTAATTGGTGAGATGGATGTGGAGAGGGAGGAACTTCCCCCACATATGACGCGTGTTCCTACAGAAGAAATCAGGAGGGCACAGAAAGTGGAAAGGGATGCTTCAGAACTAAAAGGGACTATGAGAAAAAGAATGGAGTTCCTTGACTTGGACTAG
Protein sequence

>Msa1234880

MSWAAPEDLYLSTSLASYLDKKLLILLRDGRKLMGTLRSFDQFANAVLEGACERVIVGDLYCDIPLGLYVIRGENVVLIGEMDVEREELPPHMTRVPTEEIRRAQKVERDASELKGTMRKRMEFLDLD*