AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1278960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1278960 MtrunA17_Chr8g0348961 97.326 187 5 0 1 187 262 448 1.03e-132 379
Msa1278960 MtrunA17_Chr1g0203061 63.934 183 64 2 3 184 265 446 2.33e-77 238
Msa1278960 MtrunA17_Chr3g0110381 46.667 180 85 4 1 173 262 437 4.77e-48 162
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1278960 AT4G00500.1 60.870 184 68 3 4 184 266 448 9.78e-74 229
Msa1278960 AT4G00500.2 60.870 184 68 3 4 184 266 448 9.78e-74 229
Msa1278960 AT3G49050.1 54.545 209 70 2 2 185 265 473 2.99e-71 223
Msa1278960 AT5G37710.1 46.237 186 82 3 1 183 262 432 1.79e-48 163
Msa1278960 AT5G37710.2 73.171 82 22 0 1 82 262 343 1.22e-36 130

Find 67 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATTATTTGCTATTAGTTT+TGG 0.174137 8_3:-15576199 Msa1278960:three_prime_UTR
TGATATAAAGAAATAATTTA+AGG 0.194108 8_3:+15576233 None:intergenic
AATATCATACTAAACTAAAA+TGG 0.228673 8_3:+15576140 None:intergenic
ATTGTAATTTAGAGCCTATT+TGG 0.229749 8_3:-15576077 Msa1278960:three_prime_UTR
AAACATTGTTATTTCTTTCA+TGG 0.258381 8_3:-15579304 Msa1278960:five_prime_UTR
ATTGTGTCTCATGTGCTTAA+AGG 0.265957 8_3:-15579204 Msa1278960:exon
TTTCTTTATATCAAGCTTCA+TGG 0.285716 8_3:-15576223 Msa1278960:three_prime_UTR
CTATTTGGATTAAACTTCTT+AGG 0.290416 8_3:-15576062 Msa1278960:three_prime_UTR
CTAAGAAGTTTAATCCAAAT+AGG 0.326933 8_3:+15576063 None:intergenic
GAAGATTTCCACCCATTGTT+AGG 0.336238 8_3:-15577651 Msa1278960:CDS
AGATCGGAATATGGAGATTC+CGG 0.339282 8_3:-15576534 Msa1278960:CDS
GCTAGAGAAAGATCGGAATA+TGG 0.341114 8_3:-15576543 Msa1278960:CDS
ATTGATGCTGAGGAAGATTC+TGG 0.359458 8_3:-15576377 Msa1278960:CDS
ATGAAAGAAATAACAATGTT+TGG 0.365328 8_3:+15579306 None:intergenic
TTGTCGAGCGAAAGCCCTTC+AGG 0.380817 8_3:-15579109 Msa1278960:intron
CCTTCGGAGGGCAGTTGCTT+TGG 0.387374 8_3:-15576447 Msa1278960:CDS
CTACTGACCATGCCATCCTT+TGG 0.397629 8_3:-15577573 Msa1278960:CDS
GCATACTCACCTTCCTCATA+TGG 0.405824 8_3:-15576413 Msa1278960:CDS
TTCATGAAATGTTCCATATG+AGG 0.417496 8_3:+15576400 None:intergenic
ATAATAAACGATCAGAAAGT+TGG 0.423551 8_3:-15576322 Msa1278960:CDS
ATGTTTGGAGCTCATCCTAA+TGG 0.441126 8_3:+15579321 None:intergenic
CGTGGACGACACAGGTCATA+TGG 0.445994 8_3:-15576273 Msa1278960:CDS
ATATCATACTAAACTAAAAT+GGG 0.447087 8_3:+15576141 None:intergenic
CGGCAGAGCAAAAGATGGTT+AGG 0.467187 8_3:-15576514 Msa1278960:CDS
GCTTACATCGAGAGCCCGTT+GGG 0.485898 8_3:+15577537 None:intergenic
TTGTGATGCTCAATTTAGCT+TGG 0.497889 8_3:-15576114 Msa1278960:three_prime_UTR
ATGATGGAGCTTCTGAAAGC+TGG 0.499191 8_3:+15575978 None:intergenic
GAAAGTTGGAATGAATTTGT+CGG 0.506851 8_3:-15576308 Msa1278960:CDS
GATTGAGAAGGAATCCCAAC+GGG 0.509870 8_3:-15577551 Msa1278960:CDS
ACAAGGCCAACTGCCAAGAA+AGG 0.515320 8_3:+15579231 None:intergenic
AAGAGTACCGTGCAGCCCTT+CGG 0.515446 8_3:-15576463 Msa1278960:CDS
CCAGGTGCATACAGACGCCT+TGG 0.522285 8_3:+15579146 None:intergenic
GGTACTGCTGTCCTAACAAT+GGG 0.534930 8_3:+15577640 None:intergenic
ACCACTGTAGCTCATAAAGC+TGG 0.538098 8_3:+15579260 None:intergenic
AGCTTACATCGAGAGCCCGT+TGG 0.546689 8_3:+15577536 None:intergenic
AGTTAATGCTAGAGAAAGAT+CGG 0.550817 8_3:-15576550 Msa1278960:CDS
CTGCCCTCCGAAGGGCTGCA+CGG 0.552923 8_3:+15576456 None:intergenic
CAGGTGCATACAGACGCCTT+GGG 0.556377 8_3:+15579147 None:intergenic
AGTACCGTGCAGCCCTTCGG+AGG 0.557733 8_3:-15576460 Msa1278960:CDS
TGCCATCCTTTGGATTGAGA+AGG 0.562134 8_3:-15577563 Msa1278960:CDS
TGATCAAACCTTCCATCGAC+AGG 0.566884 8_3:+15577619 None:intergenic
CCAAAGCAACTGCCCTCCGA+AGG 0.567097 8_3:+15576447 None:intergenic
CCAAGGCGTCTGTATGCACC+TGG 0.569450 8_3:-15579146 Msa1278960:CDS
CTTAAAGGATACTTGCACAC+TGG 0.573680 8_3:-15579189 Msa1278960:CDS
ATTTCATGAAATTGATGCTG+AGG 0.574640 8_3:-15576387 Msa1278960:CDS
TTCCTTCTCAATCCAAAGGA+TGG 0.575356 8_3:+15577561 None:intergenic
GGGATTCCTTCTCAATCCAA+AGG 0.576786 8_3:+15577557 None:intergenic
GAGGAAGGTGAGTATGCTTG+AGG 0.590075 8_3:+15576419 None:intergenic
AGAAGAAACTTCGAGACCCA+AGG 0.591979 8_3:-15579163 Msa1278960:CDS
GAAGATTCTGGAAGATCTAG+TGG 0.592068 8_3:-15576365 Msa1278960:CDS
CAAAGCAACTGCCCTCCGAA+GGG 0.592141 8_3:+15576448 None:intergenic
AGGTACTGCTGTCCTAACAA+TGG 0.595170 8_3:+15577639 None:intergenic
TGTCGGACGCATGTTTGACG+TGG 0.597332 8_3:-15576291 Msa1278960:CDS
TCTCAATCCAAAGGATGGCA+TGG 0.607183 8_3:+15577566 None:intergenic
CAGCAGTACCTGTCGATGGA+AGG 0.608153 8_3:-15577627 Msa1278960:CDS
TCCAGCTTTATGAGCTACAG+TGG 0.615256 8_3:-15579261 Msa1278960:five_prime_UTR
GATTCCGGCAGAGCAAAAGA+TGG 0.617790 8_3:-15576519 Msa1278960:CDS
TGAAATGTTCCATATGAGGA+AGG 0.619523 8_3:+15576404 None:intergenic
ACTGCTGTCCTAACAATGGG+TGG 0.627208 8_3:+15577643 None:intergenic
TGATCGTTTATTATGAGACA+AGG 0.629967 8_3:+15576331 None:intergenic
ATGTTTGACGTGGACGACAC+AGG 0.631845 8_3:-15576281 Msa1278960:CDS
ACAATGTGATAGAGACGACC+AGG 0.636901 8_3:+15579128 None:intergenic
AGCAGCATACTGTACCTGAA+GGG 0.650637 8_3:+15579095 None:intergenic
AGGACAGCAGTACCTGTCGA+TGG 0.651084 8_3:-15577631 Msa1278960:CDS
CATGAGACACAATATAAACA+AGG 0.655102 8_3:+15579214 None:intergenic
GGATTGAGAAGGAATCCCAA+CGG 0.658153 8_3:-15577552 Msa1278960:CDS
TCTAAATTACAATATTCACA+TGG 0.730026 8_3:+15576087 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGTTAAAATTCATAATTT+TGG + chr8_3:15576398-15576417 None:intergenic 10.0%
!!! TTTTTAAAATCTAAAACAAA+CGG + chr8_3:15576520-15576539 None:intergenic 10.0%
!!! TGTTTTAGATTTTAAAAAAA+TGG - chr8_3:15576522-15576541 Msa1278960:CDS 10.0%
!!! GTTTTTTTTAAAATAGATTT+TGG + chr8_3:15576934-15576953 None:intergenic 10.0%
!!! ACAATTTTAAACAATTTATA+TGG + chr8_3:15578645-15578664 None:intergenic 10.0%
!! TGATATAAAGAAATAATTTA+AGG + chr8_3:15579133-15579152 None:intergenic 10.0%
!!! TTTTAAAATCTAAAACAAAC+GGG + chr8_3:15576519-15576538 None:intergenic 15.0%
!!! TTCCTTTGTATTTTTAAAAA+TGG - chr8_3:15576548-15576567 Msa1278960:CDS 15.0%
!!! TTTAAGATAAACTATTCAAA+CGG - chr8_3:15576839-15576858 Msa1278960:intron 15.0%
!!! TTTTAACAAAGAAATTTTCA+AGG + chr8_3:15576884-15576903 None:intergenic 15.0%
!! TTAAAAAAAACTAGAACAAA+CGG - chr8_3:15576943-15576962 Msa1278960:intron 15.0%
!! TACTCTAGTAAAAATAAAAA+TGG - chr8_3:15577393-15577412 Msa1278960:intron 15.0%
!! ACTCTAGTAAAAATAAAAAT+GGG - chr8_3:15577394-15577413 Msa1278960:intron 15.0%
!! ATATTATATACAACTAACAA+AGG + chr8_3:15577445-15577464 None:intergenic 15.0%
!! TACAAAATTAAAATAGAAAC+TGG + chr8_3:15578064-15578083 None:intergenic 15.0%
!! AATTTCAACAATAATTTAGA+TGG - chr8_3:15578323-15578342 Msa1278960:intron 15.0%
!! TTAATTGATACATGTATATT+CGG + chr8_3:15578464-15578483 None:intergenic 15.0%
!! TAATAATAATTAAAACCTTG+AGG + chr8_3:15578751-15578770 None:intergenic 15.0%
!! ATATCATACTAAACTAAAAT+GGG + chr8_3:15579225-15579244 None:intergenic 15.0%
!! AATATCATACTAAACTAAAA+TGG + chr8_3:15579226-15579245 None:intergenic 15.0%
!!! AATTTTTCATGAATATGCAT+TGG - chr8_3:15575998-15576017 Msa1278960:three_prime_UTR 20.0%
!! ATGAAAGAAATAACAATGTT+TGG + chr8_3:15576060-15576079 None:intergenic 20.0%
!!! AAACATTGTTATTTCTTTCA+TGG - chr8_3:15576059-15576078 Msa1278960:three_prime_UTR 20.0%
!!! CTCCATTTTTAAAAATACAA+AGG + chr8_3:15576553-15576572 None:intergenic 20.0%
!! ATTATAACCAAATGATGAAA+TGG - chr8_3:15576804-15576823 Msa1278960:intron 20.0%
!! TAAAAAAAACTAGAACAAAC+GGG - chr8_3:15576944-15576963 Msa1278960:intron 20.0%
!!! TAAAATAAGTTTTGGTCATT+TGG - chr8_3:15577085-15577104 Msa1278960:intron 20.0%
!!! AAACTTATACAACTTTTCTA+TGG + chr8_3:15577421-15577440 None:intergenic 20.0%
!! TTCATTGAAACATTTAAATG+TGG + chr8_3:15578164-15578183 None:intergenic 20.0%
!!! GTTAATGATGTTTAAAATCA+TGG - chr8_3:15578521-15578540 Msa1278960:intron 20.0%
!!! CATTATTTGCTATTAGTTTT+GGG - chr8_3:15579165-15579184 Msa1278960:CDS 20.0%
!! TCTAAATTACAATATTCACA+TGG + chr8_3:15579279-15579298 None:intergenic 20.0%
! TAAGAAGAGTCAATTAATTG+CGG - chr8_3:15576457-15576476 Msa1278960:CDS 25.0%
! AACATGATGAATTTGATCTA+AGG - chr8_3:15576483-15576502 Msa1278960:CDS 25.0%
! ACATGATGAATTTGATCTAA+GGG - chr8_3:15576484-15576503 Msa1278960:CDS 25.0%
!! TTCATAAGATTTTTGCTGAA+AGG - chr8_3:15577016-15577035 Msa1278960:intron 25.0%
! TTGAGAAGAAAAAAAATCAC+AGG + chr8_3:15577114-15577133 None:intergenic 25.0%
!!! GATTTTTTTTCTTCTCAAGT+TGG - chr8_3:15577116-15577135 Msa1278960:intron 25.0%
! CACATCAACACAAAATAATT+TGG - chr8_3:15577250-15577269 Msa1278960:intron 25.0%
! ATTGTTCATAATAGAACAAC+TGG - chr8_3:15577482-15577501 Msa1278960:intron 25.0%
! ACGCATTGAATTCAATAATT+TGG + chr8_3:15577635-15577654 None:intergenic 25.0%
!!! CTTAATTCATTTTGAAACTC+TGG - chr8_3:15577923-15577942 Msa1278960:intron 25.0%
! AAGTTCAAACCAATATTTGT+TGG - chr8_3:15577968-15577987 Msa1278960:intron 25.0%
!!! CCTTATTTTCCAACAAATAT+TGG + chr8_3:15577980-15577999 None:intergenic 25.0%
!! CCAATATTTGTTGGAAAATA+AGG - chr8_3:15577977-15577996 Msa1278960:intron 25.0%
! ATGAAGTTATTTATTCATGC+AGG - chr8_3:15578179-15578198 Msa1278960:intron 25.0%
! TGAAGTTATTTATTCATGCA+GGG - chr8_3:15578180-15578199 Msa1278960:intron 25.0%
! TAGTTAATATGTTCACTGAA+TGG - chr8_3:15578365-15578384 Msa1278960:intron 25.0%
! GTTAATGCAGTTTAAATACA+TGG + chr8_3:15578623-15578642 None:intergenic 25.0%
! TAATAATTAAAACCTTGAGG+TGG + chr8_3:15578748-15578767 None:intergenic 25.0%
! ATAATAAACGATCAGAAAGT+TGG - chr8_3:15579041-15579060 Msa1278960:intron 25.0%
! TTTCTTTATATCAAGCTTCA+TGG - chr8_3:15579140-15579159 Msa1278960:CDS 25.0%
!!! GCATTATTTGCTATTAGTTT+TGG - chr8_3:15579164-15579183 Msa1278960:CDS 25.0%
!! ATTGTAATTTAGAGCCTATT+TGG - chr8_3:15579286-15579305 Msa1278960:five_prime_UTR 25.0%
! CTAAGAAGTTTAATCCAAAT+AGG + chr8_3:15579303-15579322 None:intergenic 25.0%
! CTATTTGGATTAAACTTCTT+AGG - chr8_3:15579301-15579320 Msa1278960:five_prime_UTR 25.0%
! TAAATTCAGCTTTTGCCATT+AGG - chr8_3:15576027-15576046 Msa1278960:three_prime_UTR 30.0%
CATGAGACACAATATAAACA+AGG + chr8_3:15576152-15576171 None:intergenic 30.0%
TCTTCGTAACTTTCTAATCA+TGG - chr8_3:15576427-15576446 Msa1278960:CDS 30.0%
CATGATGAATTTGATCTAAG+GGG - chr8_3:15576485-15576504 Msa1278960:CDS 30.0%
! AATTTGATCTAAGGGGATTT+TGG - chr8_3:15576492-15576511 Msa1278960:CDS 30.0%
! ATTTGATCTAAGGGGATTTT+GGG - chr8_3:15576493-15576512 Msa1278960:CDS 30.0%
TAACCAAATGATGAAATGGT+TGG - chr8_3:15576808-15576827 Msa1278960:intron 30.0%
ATTCCAACCATTTCATCATT+TGG + chr8_3:15576814-15576833 None:intergenic 30.0%
AGCAGAATTTACACGATAAA+GGG + chr8_3:15576970-15576989 None:intergenic 30.0%
TTGAATGTAACTACATGTGT+CGG - chr8_3:15577286-15577305 Msa1278960:intron 30.0%
AAGTCTACCTCACTAAATTA+CGG - chr8_3:15577561-15577580 Msa1278960:CDS 30.0%
! TTTTACCAAAACAGTAGACA+TGG - chr8_3:15577877-15577896 Msa1278960:intron 30.0%
TTAGTGTTTACTAAAGGTTG+GGG + chr8_3:15578230-15578249 None:intergenic 30.0%
GTTAGTGTTTACTAAAGGTT+GGG + chr8_3:15578231-15578250 None:intergenic 30.0%
AACTCGTTAGTGTTTACTAA+AGG + chr8_3:15578236-15578255 None:intergenic 30.0%
GATGATTCATTGATACAACA+GGG + chr8_3:15578584-15578603 None:intergenic 30.0%
TTGTATCAATGAATCATCCA+TGG - chr8_3:15578586-15578605 Msa1278960:intron 30.0%
AGTTAATGCTAGAGAAAGAT+CGG - chr8_3:15578813-15578832 Msa1278960:intron 30.0%
TTCATGAAATGTTCCATATG+AGG + chr8_3:15578966-15578985 None:intergenic 30.0%
ATTTCATGAAATTGATGCTG+AGG - chr8_3:15578976-15578995 Msa1278960:intron 30.0%
TGATCGTTTATTATGAGACA+AGG + chr8_3:15579035-15579054 None:intergenic 30.0%
GAAAGTTGGAATGAATTTGT+CGG - chr8_3:15579055-15579074 Msa1278960:intron 30.0%
ATTGTGTCTCATGTGCTTAA+AGG - chr8_3:15576159-15576178 Msa1278960:three_prime_UTR 35.0%
TGGATTACATAATGACCACA+AGG + chr8_3:15576378-15576397 None:intergenic 35.0%
GAGCAGAATTTACACGATAA+AGG + chr8_3:15576971-15576990 None:intergenic 35.0%
TTATCGTGTAAATTCTGCTC+AGG - chr8_3:15576971-15576990 Msa1278960:intron 35.0%
! TTCCAAACCCAATCATTTTG+TGG - chr8_3:15577172-15577191 Msa1278960:intron 35.0%
! TCCAAACCCAATCATTTTGT+GGG - chr8_3:15577173-15577192 Msa1278960:intron 35.0%
! AAAGTGCCAGTTATGAAACA+CGG - chr8_3:15577209-15577228 Msa1278960:intron 35.0%
CATCAAACCGTAATTTAGTG+AGG + chr8_3:15577571-15577590 None:intergenic 35.0%
!! TGCAGCAGTAATTTCTAGTT+TGG - chr8_3:15577670-15577689 Msa1278960:intron 35.0%
! CTCTTTGCTGTATCAGAATA+TGG - chr8_3:15578011-15578030 Msa1278960:intron 35.0%
CGTTAGTGTTTACTAAAGGT+TGG + chr8_3:15578232-15578251 None:intergenic 35.0%
! CATCATTAACCACGATTTTC+AGG + chr8_3:15578511-15578530 None:intergenic 35.0%
GGTTAATTGTGTTCAGTTGA+TGG - chr8_3:15578542-15578561 Msa1278960:intron 35.0%
GGATGATTCATTGATACAAC+AGG + chr8_3:15578585-15578604 None:intergenic 35.0%
ACATGGTTAATCATGTTCCA+TGG + chr8_3:15578606-15578625 None:intergenic 35.0%
TGAAATGTTCCATATGAGGA+AGG + chr8_3:15578962-15578981 None:intergenic 35.0%
!!! TTGCTATTAGTTTTGGGTCA+AGG - chr8_3:15579171-15579190 Msa1278960:CDS 35.0%
!!! TGCTATTAGTTTTGGGTCAA+GGG - chr8_3:15579172-15579191 Msa1278960:CDS 35.0%
TTGTGATGCTCAATTTAGCT+TGG - chr8_3:15579249-15579268 Msa1278960:five_prime_UTR 35.0%
! ATGTTTGGAGCTCATCCTAA+TGG + chr8_3:15576045-15576064 None:intergenic 40.0%
!! CAGTGGTTGTTTTCCTTTCT+TGG - chr8_3:15576119-15576138 Msa1278960:three_prime_UTR 40.0%
! TGTTTTCCTTTCTTGGCAGT+TGG - chr8_3:15576126-15576145 Msa1278960:three_prime_UTR 40.0%
CTTAAAGGATACTTGCACAC+TGG - chr8_3:15576174-15576193 Msa1278960:three_prime_UTR 40.0%
! TAGTTCAGTAAGGCGTCATA+TGG + chr8_3:15576333-15576352 None:intergenic 40.0%
TCGTCCTTGTTAGTTCAGTA+AGG + chr8_3:15576343-15576362 None:intergenic 40.0%
GAAGAGTCAATTAATTGCGG+AGG - chr8_3:15576460-15576479 Msa1278960:CDS 40.0%
TGAAAGGTTTACACAGCTGT+AGG - chr8_3:15577032-15577051 Msa1278960:intron 40.0%
! CCAAACCCAATCATTTTGTG+GGG - chr8_3:15577174-15577193 Msa1278960:intron 40.0%
TTGTGTCCGTGTTTCATAAC+TGG + chr8_3:15577218-15577237 None:intergenic 40.0%
AATCTTCCTAACCTGCAATC+AGG + chr8_3:15577699-15577718 None:intergenic 40.0%
GAAGATTTCCACCCATTGTT+AGG - chr8_3:15577712-15577731 Msa1278960:intron 40.0%
TTCCTTCTCAATCCAAAGGA+TGG + chr8_3:15577805-15577824 None:intergenic 40.0%
! GTATGCCATGTCTACTGTTT+TGG + chr8_3:15577885-15577904 None:intergenic 40.0%
TTCGGACATCTGCAAAATTG+TGG + chr8_3:15578446-15578465 None:intergenic 40.0%
GTAAACTCGATCACAAACTG+TGG + chr8_3:15578487-15578506 None:intergenic 40.0%
GAGTTTACACCTGAAAATCG+TGG - chr8_3:15578499-15578518 Msa1278960:intron 40.0%
GTTGAATTCTTTCCACCTCA+AGG - chr8_3:15578733-15578752 Msa1278960:intron 40.0%
GCTAGAGAAAGATCGGAATA+TGG - chr8_3:15578820-15578839 Msa1278960:intron 40.0%
AGATCGGAATATGGAGATTC+CGG - chr8_3:15578829-15578848 Msa1278960:intron 40.0%
ATTGATGCTGAGGAAGATTC+TGG - chr8_3:15578986-15579005 Msa1278960:intron 40.0%
! GAAGATTCTGGAAGATCTAG+TGG - chr8_3:15578998-15579017 Msa1278960:intron 40.0%
! TCCAGCTTTATGAGCTACAG+TGG - chr8_3:15576102-15576121 Msa1278960:three_prime_UTR 45.0%
ACCACTGTAGCTCATAAAGC+TGG + chr8_3:15576106-15576125 None:intergenic 45.0%
AGAAGAAACTTCGAGACCCA+AGG - chr8_3:15576200-15576219 Msa1278960:three_prime_UTR 45.0%
ACAATGTGATAGAGACGACC+AGG + chr8_3:15576238-15576257 None:intergenic 45.0%
AGCAGCATACTGTACCTGAA+GGG + chr8_3:15576271-15576290 None:intergenic 45.0%
GACGCCTTACTGAACTAACA+AGG - chr8_3:15576336-15576355 Msa1278960:CDS 45.0%
CGATCTTCTGATAGTCCTTG+TGG - chr8_3:15576360-15576379 Msa1278960:CDS 45.0%
TCGTGTAAATTCTGCTCAGG+AGG - chr8_3:15576974-15576993 Msa1278960:intron 45.0%
CCCCACAAAATGATTGGGTT+TGG + chr8_3:15577177-15577196 None:intergenic 45.0%
CAGGTCCCCACAAAATGATT+GGG + chr8_3:15577182-15577201 None:intergenic 45.0%
AACTGGCACTTTGTTGAAGC+AGG + chr8_3:15577201-15577220 None:intergenic 45.0%
! TACATGTGTCGGTGTCTTGT+TGG - chr8_3:15577297-15577316 Msa1278960:intron 45.0%
ACAACTGTACGTGTCAGACA+CGG - chr8_3:15577320-15577339 Msa1278960:intron 45.0%
! GATCGAAGTCTTTTCTCGCA+GGG + chr8_3:15577519-15577538 None:intergenic 45.0%
! TAGTTTGGCTTCCTGATTGC+AGG - chr8_3:15577685-15577704 Msa1278960:intron 45.0%
GGTACTGCTGTCCTAACAAT+GGG + chr8_3:15577726-15577745 None:intergenic 45.0%
! AGGTACTGCTGTCCTAACAA+TGG + chr8_3:15577727-15577746 None:intergenic 45.0%
TGATCAAACCTTCCATCGAC+AGG + chr8_3:15577747-15577766 None:intergenic 45.0%
TCTCAATCCAAAGGATGGCA+TGG + chr8_3:15577800-15577819 None:intergenic 45.0%
TGCCATCCTTTGGATTGAGA+AGG - chr8_3:15577800-15577819 Msa1278960:intron 45.0%
GGGATTCCTTCTCAATCCAA+AGG + chr8_3:15577809-15577828 None:intergenic 45.0%
GGATTGAGAAGGAATCCCAA+CGG - chr8_3:15577811-15577830 Msa1278960:intron 45.0%
GATTGAGAAGGAATCCCAAC+GGG - chr8_3:15577812-15577831 Msa1278960:intron 45.0%
! CTAACCATCTTTTGCTCTGC+CGG + chr8_3:15578851-15578870 None:intergenic 45.0%
GCATACTCACCTTCCTCATA+TGG - chr8_3:15578950-15578969 Msa1278960:intron 45.0%
!!! TTTTTTTTTAAAATAAGTTT+TGG - chr8_3:15577077-15577096 Msa1278960:intron 5.0%
ACAAGGCCAACTGCCAAGAA+AGG + chr8_3:15576135-15576154 None:intergenic 50.0%
GAGCAGCATACTGTACCTGA+AGG + chr8_3:15576272-15576291 None:intergenic 50.0%
GCAGGTCCCCACAAAATGAT+TGG + chr8_3:15577183-15577202 None:intergenic 50.0%
CAACTGTACGTGTCAGACAC+GGG - chr8_3:15577321-15577340 Msa1278960:intron 50.0%
! CGATCGAAGTCTTTTCTCGC+AGG + chr8_3:15577520-15577539 None:intergenic 50.0%
CGAGAAAAGACTTCGATCGC+TGG - chr8_3:15577521-15577540 Msa1278960:intron 50.0%
TGGCTTCCTGATTGCAGGTT+AGG - chr8_3:15577690-15577709 Msa1278960:intron 50.0%
ACTGCTGTCCTAACAATGGG+TGG + chr8_3:15577723-15577742 None:intergenic 50.0%
CTACTGACCATGCCATCCTT+TGG - chr8_3:15577790-15577809 Msa1278960:intron 50.0%
GATTCCGGCAGAGCAAAAGA+TGG - chr8_3:15578844-15578863 Msa1278960:intron 50.0%
CGGCAGAGCAAAAGATGGTT+AGG - chr8_3:15578849-15578868 Msa1278960:intron 50.0%
GAGGAAGGTGAGTATGCTTG+AGG + chr8_3:15578947-15578966 None:intergenic 50.0%
ATGTTTGACGTGGACGACAC+AGG - chr8_3:15579082-15579101 Msa1278960:intron 50.0%
CAGGTGCATACAGACGCCTT+GGG + chr8_3:15576219-15576238 None:intergenic 55.0%
TTGTCGAGCGAAAGCCCTTC+AGG - chr8_3:15576254-15576273 Msa1278960:CDS 55.0%
! CGAAGTCTTTTCTCGCAGGG+AGG + chr8_3:15577516-15577535 None:intergenic 55.0%
AGGACAGCAGTACCTGTCGA+TGG - chr8_3:15577732-15577751 Msa1278960:intron 55.0%
CAGCAGTACCTGTCGATGGA+AGG - chr8_3:15577736-15577755 Msa1278960:intron 55.0%
GCTTACATCGAGAGCCCGTT+GGG + chr8_3:15577829-15577848 None:intergenic 55.0%
AGCTTACATCGAGAGCCCGT+TGG + chr8_3:15577830-15577849 None:intergenic 55.0%
!! AAGAGTACCGTGCAGCCCTT+CGG - chr8_3:15578900-15578919 Msa1278960:intron 55.0%
CAAAGCAACTGCCCTCCGAA+GGG + chr8_3:15578918-15578937 None:intergenic 55.0%
TGTCGGACGCATGTTTGACG+TGG - chr8_3:15579072-15579091 Msa1278960:intron 55.0%
CGTGGACGACACAGGTCATA+TGG - chr8_3:15579090-15579109 Msa1278960:intron 55.0%
CCAGGTGCATACAGACGCCT+TGG + chr8_3:15576220-15576239 None:intergenic 60.0%
! CCAAGGCGTCTGTATGCACC+TGG - chr8_3:15576217-15576236 Msa1278960:three_prime_UTR 60.0%
CCAAAGCAACTGCCCTCCGA+AGG + chr8_3:15578919-15578938 None:intergenic 60.0%
CCTTCGGAGGGCAGTTGCTT+TGG - chr8_3:15578916-15578935 Msa1278960:intron 60.0%
AGTACCGTGCAGCCCTTCGG+AGG - chr8_3:15578903-15578922 Msa1278960:intron 65.0%
GTACCGTGCAGCCCTTCGGA+GGG - chr8_3:15578904-15578923 Msa1278960:intron 65.0%
CTGCCCTCCGAAGGGCTGCA+CGG + chr8_3:15578910-15578929 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 15575989 15579396 15575989 ID=Msa1278960;Name=Msa1278960
chr8_3 mRNA 15575989 15579396 15575989 ID=Msa1278960-mRNA-1;Parent=Msa1278960;Name=Msa1278960-mRNA-1;_AED=0.13;_eAED=0.13;_QI=180|1|1|1|1|1|3|261|187
chr8_3 exon 15575989 15576570 15575989 ID=Msa1278960-mRNA-1:exon:6025;Parent=Msa1278960-mRNA-1
chr8_3 exon 15577543 15577678 15577543 ID=Msa1278960-mRNA-1:exon:6024;Parent=Msa1278960-mRNA-1
chr8_3 exon 15579110 15579396 15579110 ID=Msa1278960-mRNA-1:exon:6023;Parent=Msa1278960-mRNA-1
chr8_3 five_prime_UTR 15579217 15579396 15579217 ID=Msa1278960-mRNA-1:five_prime_utr;Parent=Msa1278960-mRNA-1
chr8_3 CDS 15579110 15579216 15579110 ID=Msa1278960-mRNA-1:cds;Parent=Msa1278960-mRNA-1
chr8_3 CDS 15577543 15577678 15577543 ID=Msa1278960-mRNA-1:cds;Parent=Msa1278960-mRNA-1
chr8_3 CDS 15576250 15576570 15576250 ID=Msa1278960-mRNA-1:cds;Parent=Msa1278960-mRNA-1
chr8_3 three_prime_UTR 15575989 15576249 15575989 ID=Msa1278960-mRNA-1:three_prime_utr;Parent=Msa1278960-mRNA-1
Gene Sequence

>Msa1278960

ATGTGCTTAAAGGATACTTGCACACTGGAAGAGAAGAAACTTCGAGACCCAAGGCGTCTGTATGCACCTGGTCGTCTCTATCACATTGTCGAGCGAAAGCCCTTCAGGTTAGGAAGATTTCCACCCATTGTTAGGACAGCAGTACCTGTCGATGGAAGGTTTGATCATATAGTTCTTTCTTGCAATGCAACTACTGACCATGCCATCCTTTGGATTGAGAAGGAATCCCAACGGGCTCTCGATTTAATGCTAGAGAAAGATCGGAATATGGAGATTCCGGCAGAGCAAAAGATGGTTAGGCAAGAATCTCTAGCTCGCGAACACAGTGAAGAGTACCGTGCAGCCCTTCGGAGGGCAGTTGCTTTGGATATTCCTCAAGCATACTCACCTTCCTCATATGGAACATTTCATGAAATTGATGCTGAGGAAGATTCTGGAAGATCTAGTGGCAAAGACTCTTCCTTGTCTCATAATAAACGATCAGAAAGTTGGAATGAATTTGTCGGACGCATGTTTGACGTGGACGACACAGGTCATATGGTGTTCAAGAAGACAACTCCTTAA
Protein sequence

>Msa1278960

MCLKDTCTLEEKKLRDPRRLYAPGRLYHIVERKPFRLGRFPPIVRTAVPVDGRFDHIVLSCNATTDHAILWIEKESQRALDLMLEKDRNMEIPAEQKMVRQESLAREHSEEYRAALRRAVALDIPQAYSPSSYGTFHEIDAEEDSGRSSGKDSSLSHNKRSESWNEFVGRMFDVDDTGHMVFKKTTP*