AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1283050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1283050 MtrunA17_Chr8g0354711 85.087 751 105 5 1 745 717 1466 0.0 1276
Msa1283050 MtrunA17_Chr8g0354701 68.809 747 231 2 1 746 722 1467 0.0 1030
Msa1283050 MtrunA17_Chr8g0354721 54.684 790 293 11 1 744 721 1491 0.0 764
Msa1283050 MtrunA17_Chr8g0354681 60.299 335 120 4 1 329 725 1052 6.11e-115 372
Msa1283050 MtrunA17_Chr8g0354671 49.482 386 146 4 402 743 2 382 6.44e-104 323
Msa1283050 MtrunA17_Chr8g0353371 45.263 380 190 7 1 367 733 1107 3.55e-83 286
Msa1283050 MtrunA17_Chr8g0371711 37.903 372 170 9 1 367 724 1039 9.28e-63 227
Msa1283050 MtrunA17_Chr5g0415831 36.243 378 175 9 1 367 91 413 1.06e-54 194
Msa1283050 MtrunA17_Chr8g0379351 37.377 305 140 3 2 304 250 505 1.09e-51 187
Msa1283050 MtrunA17_Chr6g0472561 34.817 382 181 11 1 371 1 325 4.32e-51 182
Msa1283050 MtrunA17_Chr6g0480981 34.865 370 178 12 3 367 463 774 1.90e-50 189
Msa1283050 MtrunA17_Chr6g0481011 35.356 379 175 11 1 371 735 1051 1.77e-48 185
Msa1283050 MtrunA17_Chr6g0482141 33.248 391 181 10 1 382 893 1212 1.79e-46 179
Msa1283050 MtrunA17_Chr6g0482511 34.545 385 173 11 1 367 749 1072 1.04e-45 176
Msa1283050 MtrunA17_Chr3g0077971 34.133 375 164 10 1 368 1260 1558 1.44e-45 176
Msa1283050 MtrunA17_Chr6g0482461 33.777 376 169 13 1 368 374 677 8.88e-44 168
Msa1283050 MtrunA17_Chr3g0084151 32.723 382 189 8 1 365 703 1033 3.75e-43 168
Msa1283050 MtrunA17_Chr6g0482491 31.367 373 190 6 1 367 964 1276 9.14e-43 167
Msa1283050 MtrunA17_Chr1g0172541 31.152 382 182 10 1 376 836 1142 1.68e-42 166
Msa1283050 MtrunA17_Chr3g0081531 23.753 762 459 19 1 727 709 1383 2.79e-41 163
Msa1283050 MtrunA17_Chr6g0480911 33.245 379 188 9 1 369 731 1054 2.03e-40 160
Msa1283050 MtrunA17_Chr6g0482351 32.258 372 179 12 1 368 1176 1478 1.17e-39 158
Msa1283050 MtrunA17_Chr8g0350801 32.447 376 162 11 1 367 785 1077 2.00e-39 157
Msa1283050 MtrunA17_Chr6g0482321 40.234 256 124 10 127 372 913 1149 2.58e-39 157
Msa1283050 MtrunA17_Chr6g0482441 31.818 374 173 13 1 368 978 1275 6.86e-38 152
Msa1283050 MtrunA17_Chr6g0451551 29.708 377 184 11 1 368 867 1171 2.59e-36 147
Msa1283050 MtrunA17_Chr8g0353381 42.945 163 83 5 210 367 1 158 3.27e-36 134
Msa1283050 MtrunA17_Chr4g0006771 35.069 288 135 4 1 282 707 948 2.21e-35 144
Msa1283050 MtrunA17_Chr2g0300251 31.358 405 204 13 1 390 711 1056 1.95e-33 138
Msa1283050 MtrunA17_Chr6g0482111 29.600 375 200 8 1 367 719 1037 4.55e-32 134
Msa1283050 MtrunA17_Chr6g0479631 28.571 357 193 9 14 358 697 1003 5.36e-32 134
Msa1283050 MtrunA17_Chr6g0481231 29.744 390 182 11 1 373 101 415 7.95e-32 129
Msa1283050 MtrunA17_Chr6g0481251 28.894 398 183 13 1 377 565 883 2.82e-31 131
Msa1283050 MtrunA17_Chr6g0481141 26.700 397 195 9 1 377 758 1078 3.36e-30 128
Msa1283050 MtrunA17_Chr6g0484161 29.668 391 176 13 1 369 727 1040 3.91e-30 127
Msa1283050 MtrunA17_Chr6g0481001 34.615 208 113 9 175 371 775 970 1.42e-29 126
Msa1283050 MtrunA17_Chr6g0480051 30.851 376 188 11 1 366 483 796 8.87e-29 123
Msa1283050 MtrunA17_Chr6g0480041 29.262 393 183 13 1 373 723 1040 1.04e-28 123
Msa1283050 MtrunA17_Chr6g0479841 29.674 337 173 10 14 337 350 635 4.32e-28 120
Msa1283050 MtrunA17_Chr6g0479001 28.463 397 188 14 1 375 691 1013 2.94e-27 119
Msa1283050 MtrunA17_Chr6g0479591 29.620 395 184 15 21 373 653 995 3.82e-27 118
Msa1283050 MtrunA17_Chr1g0153511 50.769 130 59 2 1 127 1284 1411 4.37e-27 118
Msa1283050 MtrunA17_Chr6g0480421 30.423 378 162 15 1 367 566 853 2.80e-26 115
Msa1283050 MtrunA17_Chr6g0478451 30.208 384 180 13 1 367 710 1022 5.24e-26 114
Msa1283050 MtrunA17_Chr6g0479671 30.273 403 197 16 1 380 706 1047 6.20e-26 114
Msa1283050 MtrunA17_Chr6g0480451 29.925 401 196 20 1 381 743 1078 9.32e-26 114
Msa1283050 MtrunA17_Chr6g0479641 29.897 388 170 14 1 367 709 1015 1.57e-25 113
Msa1283050 MtrunA17_Chr6g0479621 28.713 404 176 15 1 367 703 1031 1.66e-25 113
Msa1283050 MtrunA17_Chr6g0479741 29.560 318 145 13 74 367 35 297 1.88e-25 107
Msa1283050 MtrunA17_Chr6g0480401 32.271 251 97 5 1 247 202 383 4.75e-25 108
Msa1283050 MtrunA17_Chr6g0480321 29.737 380 176 16 1 367 562 863 9.95e-25 110
Msa1283050 MtrunA17_Chr6g0478841 29.189 370 193 13 14 369 682 996 1.65e-24 110
Msa1283050 MtrunA17_Chr4g0025831 25.858 379 188 12 1 367 704 1001 9.38e-24 107
Msa1283050 MtrunA17_Chr6g0479051 26.997 363 174 10 1 353 719 1000 1.72e-23 106
Msa1283050 MtrunA17_Chr1g0153501 37.654 162 79 8 212 368 1 145 7.21e-23 95.5
Msa1283050 MtrunA17_Chr6g0480861 40.449 178 94 3 1 177 1 167 8.73e-23 96.3
Msa1283050 MtrunA17_Chr5g0417051 31.873 251 124 7 1 248 75 281 1.01e-22 99.0
Msa1283050 MtrunA17_Chr6g0480521 27.128 376 182 14 1 367 705 997 1.19e-22 103
Msa1283050 MtrunA17_Chr1g0146421 27.154 383 188 12 1 367 701 1008 2.87e-22 102
Msa1283050 MtrunA17_Chr6g0478771 29.644 253 104 5 1 247 692 876 3.40e-22 102
Msa1283050 MtrunA17_Chr6g0479651 27.344 384 201 10 1 366 715 1038 3.87e-22 102
Msa1283050 MtrunA17_Chr6g0479701 28.814 354 175 13 3 327 623 928 3.97e-22 102
Msa1283050 MtrunA17_Chr1g0181021 27.134 328 158 9 74 369 27 305 5.09e-22 97.4
Msa1283050 MtrunA17_Chr6g0479821 38.286 175 84 9 167 327 759 923 6.54e-22 101
Msa1283050 MtrunA17_Chr5g0417071 28.814 295 140 9 84 367 308 543 2.68e-21 98.6
Msa1283050 MtrunA17_Chr5g0442761 26.684 386 224 12 1 367 718 1063 6.47e-21 98.6
Msa1283050 MtrunA17_Chr8g0368251 26.753 385 183 14 1 367 703 1006 2.14e-20 96.7
Msa1283050 MtrunA17_Chr4g0025771 26.065 399 227 17 20 367 88 469 3.39e-20 95.1
Msa1283050 MtrunA17_Chr6g0480311 27.841 352 168 11 16 354 709 987 7.52e-20 95.1
Msa1283050 MtrunA17_Chr4g0010521 26.131 398 205 14 1 369 710 1047 5.73e-19 92.4
Msa1283050 MtrunA17_Chr4g0025691 28.622 283 155 13 101 367 40 291 1.10e-18 88.6
Msa1283050 MtrunA17_Chr4g0010651 25.952 420 215 17 1 387 712 1068 2.43e-18 90.5
Msa1283050 MtrunA17_Chr4g0025811 24.250 400 229 14 20 367 626 1003 6.73e-18 89.0
Msa1283050 MtrunA17_Chr6g0479761 36.943 157 74 2 1 156 565 697 2.29e-17 86.7
Msa1283050 MtrunA17_Chr6g0478741 34.756 164 76 2 1 159 671 808 1.05e-16 84.7
Msa1283050 MtrunA17_Chr6g0480361 35.047 214 111 13 167 367 247 445 2.71e-16 82.4
Msa1283050 MtrunA17_Chr6g0480931 37.662 154 79 9 223 369 1 144 2.44e-15 74.3
Msa1283050 MtrunA17_Chr6g0480961 42.105 76 42 2 210 283 1 76 5.99e-15 70.9
Msa1283050 MtrunA17_Chr4g0025751 30.000 260 121 10 20 249 641 869 5.30e-14 76.3
Msa1283050 MtrunA17_Chr8g0363891 26.378 254 162 6 1 239 303 546 2.72e-13 73.6
Msa1283050 MtrunA17_Chr6g0465271 50.877 57 25 2 207 260 11 67 1.21e-12 63.9
Msa1283050 MtrunA17_Chr7g0248121 29.348 276 136 9 4 260 794 1029 2.94e-12 70.5
Msa1283050 MtrunA17_Chr8g0363851 28.175 252 143 7 14 246 781 1013 9.51e-12 68.9
Msa1283050 MtrunA17_Chr4g0025741 32.857 140 89 2 14 153 692 826 1.23e-11 68.6
Msa1283050 MtrunA17_Chr2g0281531 36.842 133 66 3 1 132 267 382 2.85e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1283050 AT1G27180.1 28.854 253 160 7 1 237 1148 1396 1.23e-14 79.0
Msa1283050 AT1G27180.2 28.854 253 160 7 1 237 986 1234 1.36e-14 78.6
Msa1283050 AT1G27180.5 28.854 253 160 7 1 237 984 1232 1.44e-14 78.6
Msa1283050 AT1G27180.8 28.854 253 160 7 1 237 984 1232 1.44e-14 78.6
Msa1283050 AT1G27180.7 28.854 253 160 7 1 237 810 1058 1.61e-14 78.6
Msa1283050 AT1G27180.6 28.854 253 160 7 1 237 810 1058 1.61e-14 78.6
Msa1283050 AT1G27180.4 28.854 253 160 7 1 237 810 1058 1.61e-14 78.6
Msa1283050 AT1G27170.1 28.063 253 162 7 1 237 974 1222 2.69e-13 74.3
Msa1283050 AT1G27170.2 28.063 253 162 7 1 237 974 1222 3.41e-13 73.9
Msa1283050 AT5G41740.1 25.902 305 192 12 16 309 650 931 8.60e-13 72.8
Msa1283050 AT5G41740.3 25.902 305 192 12 16 309 498 779 9.94e-13 72.4
Msa1283050 AT5G41740.2 25.902 305 192 12 16 309 650 931 1.29e-12 72.0
Msa1283050 AT5G45230.1 24.233 326 208 11 2 303 748 1058 1.79e-12 71.6
Msa1283050 AT5G17880.2 25.000 364 213 16 2 341 750 1077 4.71e-12 70.5
Msa1283050 AT5G17880.1 25.000 364 213 16 2 341 750 1077 5.70e-12 70.1
Msa1283050 AT5G36930.4 30.481 187 115 3 1 185 715 888 6.75e-12 69.7
Msa1283050 AT5G36930.1 30.481 187 115 3 1 185 715 888 6.75e-12 69.7
Msa1283050 AT5G36930.2 30.481 187 115 3 1 185 718 891 6.93e-12 69.7
Msa1283050 AT5G36930.3 30.481 187 115 3 1 185 715 888 7.35e-12 69.7
Msa1283050 AT5G45200.1 26.974 304 168 12 2 265 752 1041 2.23e-11 68.2
Msa1283050 AT5G45060.2 24.101 278 176 9 2 265 726 982 3.57e-11 67.4
Msa1283050 AT5G45060.1 24.101 278 176 9 2 265 726 982 3.57e-11 67.4
Msa1283050 AT5G45200.2 26.974 304 168 12 2 265 508 797 4.16e-11 67.4

Find 169 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCATCTTCCCCAATATTTC+TGG 0.129400 8_3:-24247471 None:intergenic
GTGGAGATTCAATATCTTTC+TGG 0.141684 8_3:+24248582 Msa1283050:CDS
CAAAGAAAACATCTTGTTTC+CGG 0.169239 8_3:-24248519 None:intergenic
TACATGGATTCCATTATATT+TGG 0.181653 8_3:-24248900 None:intergenic
GCACAGTGGTTTGGTCTTCC+TGG 0.214345 8_3:-24249133 None:intergenic
ACTGGAGTTATTGATGATTA+AGG 0.232756 8_3:+24249541 Msa1283050:CDS
CACAGTCTCGTGCTGAATTC+TGG 0.251793 8_3:-24249771 None:intergenic
ATTTGTCTGTGCCTTGTTAT+TGG 0.253995 8_3:+24248626 Msa1283050:CDS
CTTCAATTCAAAACCTTAAT+CGG 0.254512 8_3:+24247544 Msa1283050:CDS
CGAGAGTTTCCCAGAAATAT+TGG 0.259548 8_3:+24247461 Msa1283050:five_prime_UTR
ATCTTTGAGAGAACTTATTC+TGG 0.269120 8_3:+24247995 Msa1283050:CDS
GCACGCAGTCATCTATCTTT+AGG 0.274344 8_3:-24247875 None:intergenic
CACATTTACCAATGTTTGTT+GGG 0.297193 8_3:-24248963 None:intergenic
TTCATCTCTATTGTCACCTT+GGG 0.297294 8_3:-24249644 None:intergenic
ATCCAAGAGCTGACGAAGTT+TGG 0.303358 8_3:-24249600 None:intergenic
TGCAAGCTTGTTAGTGAATC+AGG 0.310293 8_3:+24248109 Msa1283050:CDS
TCTCCCAAAGGCATCTTTAC+TGG 0.311797 8_3:-24249236 None:intergenic
TATTTGGCAACCACTTTGAT+TGG 0.323181 8_3:-24248884 None:intergenic
TTTGTCTGTGCCTTGTTATT+GGG 0.323547 8_3:+24248627 Msa1283050:CDS
TTGCCATTGAGGAACACTTT+GGG 0.333133 8_3:-24248686 None:intergenic
GGCTGAAAATAGTAGGAAAA+AGG 0.333719 8_3:+24249856 Msa1283050:CDS
GTCCTTCCATTGATCCTTGC+CGG 0.338475 8_3:-24249795 None:intergenic
TGCTAAAATTTGAAGATAAC+GGG 0.340618 8_3:+24248786 Msa1283050:CDS
CACTCTGGAATCTTTGTTCC+TGG 0.341186 8_3:-24248542 None:intergenic
GTTTGCCAATGTGAAAGATT+TGG 0.346979 8_3:+24247788 Msa1283050:CDS
AAAGTGGTTGCCAAATATAA+TGG 0.350054 8_3:+24248890 Msa1283050:CDS
GAGAGTTTCCCAGAAATATT+GGG 0.352225 8_3:+24247462 Msa1283050:five_prime_UTR
GGCAAGAAAACCATATCTTC+TGG 0.353901 8_3:+24249467 Msa1283050:CDS
CACAGTGGTTTGGTCTTCCT+GGG 0.354035 8_3:-24249132 None:intergenic
ATCAGGTAGTCTACAAGTTC+AGG 0.358501 8_3:+24248726 Msa1283050:CDS
AAGATGAAAGAAAACATTCT+TGG 0.366204 8_3:+24250154 Msa1283050:CDS
ATTTCCAGTAAAGATGCCTT+TGG 0.366306 8_3:+24249232 Msa1283050:CDS
AGAACTTACCCCAGAAGATA+TGG 0.370636 8_3:-24249477 None:intergenic
CTTCATCTCTATTGTCACCT+TGG 0.372573 8_3:-24249645 None:intergenic
TCCTTCCCATAGGTCTGTCT+TGG 0.378407 8_3:+24247766 Msa1283050:CDS
GGTGACCCCACGATTGAATC+AGG 0.380402 8_3:-24248995 None:intergenic
CCATCTTCCCCAATATTTCT+GGG 0.382320 8_3:-24247470 None:intergenic
TTCCAAACTTCGTCAGCTCT+TGG 0.382450 8_3:+24249598 Msa1283050:CDS
TCTTATTAAGCTGGCCGGTT+AGG 0.388508 8_3:-24250135 None:intergenic
TTAACAGAGCTTCGTATAAT+CGG 0.391776 8_3:+24247498 Msa1283050:CDS
CCCAGAAATATTGGGGAAGA+TGG 0.394468 8_3:+24247470 Msa1283050:exon
GCAGAGAAATGATCCAAGTT+TGG 0.396675 8_3:-24248056 None:intergenic
TGTAAGGTCTATGTATTTCA+AGG 0.398123 8_3:-24247961 None:intergenic
TGTGTTGAATCTCAAGAATC+AGG 0.398962 8_3:+24249946 Msa1283050:CDS
GTGAAAGATTTGGACCTATC+CGG 0.403332 8_3:+24247798 Msa1283050:CDS
AAGAATAGTGAAATTATTCC+CGG 0.403620 8_3:-24247817 None:intergenic
AGGATCTTATTACCAACAAT+GGG 0.411889 8_3:+24248858 Msa1283050:CDS
AGTAGCATAAGCTTCATTAT+CGG 0.413443 8_3:-24249288 None:intergenic
TGACAGGGTTCTGCCAAACT+TGG 0.414150 8_3:+24248043 Msa1283050:CDS
GGGCTAAACTTGACTGAGAT+AGG 0.418290 8_3:-24248665 None:intergenic
GAAGGTTTCTTTGTACATCA+AGG 0.418734 8_3:+24249835 Msa1283050:CDS
CGAACAGTCCAGTGGCAATC+AGG 0.418755 8_3:+24248709 Msa1283050:CDS
TCCCATTCTTCACTTATGTC+CGG 0.421817 8_3:-24247684 None:intergenic
TCACATTTACCAATGTTTGT+TGG 0.421838 8_3:-24248964 None:intergenic
AATAGAAAACTATGCGTTAG+TGG 0.421946 8_3:+24249446 Msa1283050:CDS
AAGATTGACATTCTTCTGTA+AGG 0.427098 8_3:-24247977 None:intergenic
TTTCCAGTAAAGATGCCTTT+GGG 0.434197 8_3:+24249233 Msa1283050:CDS
TTTCGAGATGTGAAGGGTTG+TGG 0.435104 8_3:+24247652 Msa1283050:CDS
TTGATTTCAGAGGGTGGAGA+AGG 0.435200 8_3:-24249185 None:intergenic
TACTACTTTGTTGAGATACA+TGG 0.435509 8_3:-24248916 None:intergenic
TCAAAGAGTGTCACTTTCTC+AGG 0.436276 8_3:+24247850 Msa1283050:CDS
ATGCTAAAATTTGAAGATAA+CGG 0.437875 8_3:+24248785 Msa1283050:CDS
TCATGTGGTGGTTTCATATG+AGG 0.442036 8_3:+24248838 Msa1283050:CDS
TTTATCTTCGAGAAATTGAC+AGG 0.444611 8_3:+24248027 Msa1283050:CDS
TTTCTTTCATCTTATTAAGC+TGG 0.454296 8_3:-24250144 None:intergenic
TTTGTTGGGGATTGCTGAAT+CGG 0.455265 8_3:-24248949 None:intergenic
ACCGGACATAAGTGAAGAAT+GGG 0.456105 8_3:+24247683 Msa1283050:CDS
GTTGCCATTGAGGAACACTT+TGG 0.458585 8_3:-24248687 None:intergenic
TTCAATCCTGATTCAATCGT+GGG 0.461007 8_3:+24248989 Msa1283050:CDS
GCAAGAAAACCATATCTTCT+GGG 0.462088 8_3:+24249468 Msa1283050:CDS
TAGAGAGACTATTAATTCAC+TGG 0.463466 8_3:+24249523 Msa1283050:CDS
TTTAAGAGAGGTCAATATGA+TGG 0.466004 8_3:-24247409 None:intergenic
ATCATCCGAGTCAGAACCTT+CGG 0.466988 8_3:-24249408 None:intergenic
ACATCAAGGCTGAAAATAGT+AGG 0.467304 8_3:+24249849 Msa1283050:CDS
CTGTGGCCGGCAAGGATCAA+TGG 0.470479 8_3:+24249789 Msa1283050:CDS
TTTGATTGGAACCCCATTGT+TGG 0.471015 8_3:-24248870 None:intergenic
ATATCTTCTGGGGTAAGTTC+TGG 0.471326 8_3:+24249479 Msa1283050:CDS
AACCGGACATAAGTGAAGAA+TGG 0.473396 8_3:+24247682 Msa1283050:CDS
ACAAGGCACAGACAAATTGC+AGG 0.478724 8_3:-24248620 None:intergenic
TGAAAGATTTGGACCTATCC+GGG 0.481393 8_3:+24247799 Msa1283050:CDS
AGAGTAGGCTCAAGTGGACT+TGG 0.482501 8_3:+24250104 Msa1283050:CDS
AGCCCCAAAGTGTTCCTCAA+TGG 0.485552 8_3:+24248683 Msa1283050:CDS
AGGGAGACAGCACAGTGGTT+TGG 0.488924 8_3:-24249142 None:intergenic
AGGGTTGTGGTTATCTAAAC+CGG 0.489591 8_3:+24247665 Msa1283050:CDS
ACTTGGTCTATTGACCTAAC+CGG 0.494695 8_3:+24250121 Msa1283050:CDS
TAGAGCAGAGTAGGCTCAAG+TGG 0.496961 8_3:+24250098 Msa1283050:CDS
CAGCTGAAATAGAGCAGAGT+AGG 0.498400 8_3:+24250089 Msa1283050:CDS
ACAGCTAGCTTCCAAAGAAA+AGG 0.499290 8_3:+24249910 Msa1283050:CDS
GAAGAATTATGAGAATCATC+TGG 0.505535 8_3:+24249748 Msa1283050:CDS
AAATTCTGTCTTCGAAGACT+TGG 0.512096 8_3:-24247709 None:intergenic
GATTCAACACAAGTTGTTCG+AGG 0.516813 8_3:-24249934 None:intergenic
CGAGTCAGAACCTTCGGTGT+TGG 0.518580 8_3:-24249402 None:intergenic
TCACAGATTCAACAGTGGCT+TGG 0.518584 8_3:-24249718 None:intergenic
GGCATCTTTACTGGAAATGT+AGG 0.519141 8_3:-24249227 None:intergenic
GAAAACATGGCAATGCTACT+TGG 0.520021 8_3:-24247609 None:intergenic
TCTGTGCCTTGTTATTGGGC+AGG 0.525049 8_3:+24248631 Msa1283050:CDS
GAAAGTGTTTCTAATTTGCA+TGG 0.526053 8_3:-24247921 None:intergenic
AAAGATGCCTTTGGGAGATG+TGG 0.528159 8_3:+24249241 Msa1283050:CDS
CAAGGTGACAATAGAGATGA+AGG 0.529920 8_3:+24249646 Msa1283050:CDS
GGCAAACCAAGACAGACCTA+TGG 0.529986 8_3:-24247772 None:intergenic
GGAAGAAGACTCTTCTTCAC+AGG 0.533956 8_3:-24249381 None:intergenic
TTTCTATTAACTAAGTCGAA+GGG 0.537656 8_3:-24249431 None:intergenic
CAGCAATCCCCAACAAACAT+TGG 0.541571 8_3:+24248955 Msa1283050:CDS
ATGTGTGTTTCGAGATGTGA+AGG 0.542705 8_3:+24247645 Msa1283050:CDS
TCTCTATTGTCACCTTGGGG+TGG 0.549648 8_3:-24249640 None:intergenic
GAGGATCTTATTACCAACAA+TGG 0.554704 8_3:+24248857 Msa1283050:CDS
TAGTGAAATTATTCCCGGAT+AGG 0.556157 8_3:-24247812 None:intergenic
CGACAGGAAATGATTGAAGC+CGG 0.557548 8_3:+24248500 Msa1283050:intron
TGCCATTGAGGAACACTTTG+GGG 0.561919 8_3:-24248685 None:intergenic
AGAGGGTGATGATGTCGAGC+TGG 0.564077 8_3:+24249349 Msa1283050:CDS
TATTGCTGAGTTCAAGAGAA+AGG 0.566425 8_3:+24249673 Msa1283050:CDS
TCAGATGATTTCCTTCCCAT+AGG 0.566951 8_3:+24247756 Msa1283050:CDS
ACATTTACCAATGTTTGTTG+GGG 0.567699 8_3:-24248962 None:intergenic
CAAACCAAGTTGTGAAAACA+TGG 0.570025 8_3:-24247622 None:intergenic
TTTCATCTTATTAAGCTGGC+CGG 0.571028 8_3:-24250140 None:intergenic
CATCCGCCTGCCCAATAACA+AGG 0.573139 8_3:-24248637 None:intergenic
TGGACTGTTCGTTGCCATTG+AGG 0.573399 8_3:-24248697 None:intergenic
ATATTGAATCTCCACTTGTA+CGG 0.575170 8_3:-24248574 None:intergenic
GTAGACTACCTGATTGCCAC+TGG 0.579096 8_3:-24248717 None:intergenic
ATTCAGCACGAGACTGTGGC+CGG 0.579952 8_3:+24249776 Msa1283050:CDS
AGAGAGGTCAATATGATGGG+TGG 0.580171 8_3:-24247405 None:intergenic
GGGGTCACCTCAAAGAGTAA+AGG 0.582126 8_3:+24249009 Msa1283050:CDS
AAGATGCCTTTGGGAGATGT+GGG 0.586108 8_3:+24249242 Msa1283050:CDS
AGAACTACAAATCTGTGGAA+TGG 0.586446 8_3:+24247578 Msa1283050:CDS
CTCATTCAAAGATGTAGAGA+GGG 0.586658 8_3:+24249332 Msa1283050:CDS
TCTCTACATCTTTGAATGAG+AGG 0.587511 8_3:-24249329 None:intergenic
CAACACCGAAGGTTCTGACT+CGG 0.591531 8_3:+24249403 Msa1283050:CDS
TTTCAATCCTGATTCAATCG+TGG 0.595064 8_3:+24248988 Msa1283050:CDS
GAATTCATGAAATGATGCAA+AGG 0.595202 8_3:-24249696 None:intergenic
CGAAGTTTGGAATGTGCGGA+AGG 0.596748 8_3:-24249587 None:intergenic
TTAAGAGAGGTCAATATGAT+GGG 0.598217 8_3:-24247408 None:intergenic
AGATTGTGTTGATGTGAGTG+AGG 0.603379 8_3:-24249162 None:intergenic
ACAGTGGTTTGGTCTTCCTG+GGG 0.606601 8_3:-24249131 None:intergenic
GCAAACCAAGACAGACCTAT+GGG 0.611839 8_3:-24247771 None:intergenic
TCATCTCTATTGTCACCTTG+GGG 0.613937 8_3:-24249643 None:intergenic
CAGAATTCAGCACGAGACTG+TGG 0.614851 8_3:+24249772 Msa1283050:CDS
ATCATCTGATAAATTGCAGT+AGG 0.618009 8_3:-24247742 None:intergenic
GAGTCTTCTTCCAACACCGA+AGG 0.618932 8_3:+24249392 Msa1283050:CDS
TCTCATTCAAAGATGTAGAG+AGG 0.619473 8_3:+24249331 Msa1283050:CDS
GTACGGTGTGAAAACCACTC+TGG 0.623125 8_3:-24248557 None:intergenic
TAGGTCCAAATCTTTCACAT+TGG 0.626169 8_3:-24247793 None:intergenic
AAATTAGAACTACAAATCTG+TGG 0.626177 8_3:+24247573 Msa1283050:CDS
TCTCTTCCCACATCTCCCAA+AGG 0.627588 8_3:-24249248 None:intergenic
CTGACGAAGTTTGGAATGTG+CGG 0.628337 8_3:-24249591 None:intergenic
AAAGTTGAGCTGTTTAAGAG+AGG 0.629374 8_3:-24247421 None:intergenic
GGCCGGCAAGGATCAATGGA+AGG 0.631326 8_3:+24249793 Msa1283050:CDS
GTGCCTTGTTATTGGGCAGG+CGG 0.633594 8_3:+24248634 Msa1283050:CDS
GATTATGACTAAGAGACCCC+AGG 0.634497 8_3:+24249115 Msa1283050:intron
TGTGTGTTTCGAGATGTGAA+GGG 0.635378 8_3:+24247646 Msa1283050:CDS
ACCAAGACAGACCTATGGGA+AGG 0.641967 8_3:-24247767 None:intergenic
GAGTGCAAGTAGCCACCCCA+AGG 0.646264 8_3:+24249628 Msa1283050:CDS
AGCGGTGAGATAAAACATGA+TGG 0.648523 8_3:+24250061 Msa1283050:CDS
CAAGAAAACCATATCTTCTG+GGG 0.650883 8_3:+24249469 Msa1283050:CDS
CAATGGGGTTCCAATCAAAG+TGG 0.653320 8_3:+24248874 Msa1283050:CDS
TCAATCCTGATTCAATCGTG+GGG 0.660470 8_3:+24248990 Msa1283050:CDS
CAAGGCACAGACAAATTGCA+GGG 0.661851 8_3:-24248619 None:intergenic
GATTGTGTTGATGTGAGTGA+GGG 0.663041 8_3:-24249161 None:intergenic
AGAGTTTCCCAGAAATATTG+GGG 0.664942 8_3:+24247463 Msa1283050:five_prime_UTR
GCTAAAATTTGAAGATAACG+GGG 0.694140 8_3:+24248787 Msa1283050:CDS
GATGATCTACTTGCTTCAAG+CGG 0.694403 8_3:+24250043 Msa1283050:CDS
CAGGAACAAAGATTCCAGAG+TGG 0.696773 8_3:+24248543 Msa1283050:CDS
AATGGCAACGAACAGTCCAG+TGG 0.699808 8_3:+24248701 Msa1283050:CDS
TTATCTTCGAGAAATTGACA+GGG 0.715676 8_3:+24248028 Msa1283050:CDS
CAATGAGTGGAATCATGTGG+TGG 0.723623 8_3:+24248826 Msa1283050:CDS
GGTGACAATAGAGATGAAGG+AGG 0.725003 8_3:+24249649 Msa1283050:CDS
CTTACTCACAGATTCAACAG+TGG 0.730006 8_3:-24249723 None:intergenic
GGATCTTATTACCAACAATG+GGG 0.731369 8_3:+24248859 Msa1283050:CDS
AGACAATGAGTGGAATCATG+TGG 0.742228 8_3:+24248823 Msa1283050:CDS
GAGTGAGGGAGACAGCACAG+TGG 0.813091 8_3:-24249147 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTCATGTTGATTATTA+TGG + chr8_3:24248187-24248206 Msa1283050:intron 15.0%
!! AAATGTTAGAAACTTATAAA+AGG - chr8_3:24248349-24248368 None:intergenic 15.0%
!! AACTTATAAAAGGTTACTAT+GGG - chr8_3:24248339-24248358 None:intergenic 20.0%
!! AAACTTATAAAAGGTTACTA+TGG - chr8_3:24248340-24248359 None:intergenic 20.0%
!! TAATATCAGTTTCTATCAAA+TGG + chr8_3:24248413-24248432 Msa1283050:intron 20.0%
!! AATATCAGTTTCTATCAAAT+GGG + chr8_3:24248414-24248433 Msa1283050:intron 20.0%
!!! ATCATATACTTTTGTTTGAT+GGG + chr8_3:24248759-24248778 Msa1283050:CDS 20.0%
!! ATGCTAAAATTTGAAGATAA+CGG + chr8_3:24248785-24248804 Msa1283050:CDS 20.0%
!! CTTCAAAAAATCAATAAAGA+AGG + chr8_3:24249817-24249836 Msa1283050:CDS 20.0%
!!! TTAAGGTTTTGAATTGAAGA+TGG - chr8_3:24247543-24247562 None:intergenic 25.0%
! CTTCAATTCAAAACCTTAAT+CGG + chr8_3:24247544-24247563 Msa1283050:CDS 25.0%
! AAATTAGAACTACAAATCTG+TGG + chr8_3:24247573-24247592 Msa1283050:CDS 25.0%
! AAGAATAGTGAAATTATTCC+CGG - chr8_3:24247820-24247839 None:intergenic 25.0%
! AATAAAACAGAGATCAAAAG+AGG - chr8_3:24248137-24248156 None:intergenic 25.0%
! ACTTATAAAAGGTTACTATG+GGG - chr8_3:24248338-24248357 None:intergenic 25.0%
!! TAGAAGTTTGAGATATTGAT+AGG - chr8_3:24248448-24248467 None:intergenic 25.0%
!!! GATCATATACTTTTGTTTGA+TGG + chr8_3:24248758-24248777 Msa1283050:CDS 25.0%
! TGCTAAAATTTGAAGATAAC+GGG + chr8_3:24248786-24248805 Msa1283050:CDS 25.0%
! TACATGGATTCCATTATATT+TGG - chr8_3:24248903-24248922 None:intergenic 25.0%
!! AATTTTACCTTTACTCTTTG+AGG - chr8_3:24249019-24249038 None:intergenic 25.0%
! TTTCTATTAACTAAGTCGAA+GGG - chr8_3:24249434-24249453 None:intergenic 25.0%
!! TTTTCTATTAACTAAGTCGA+AGG - chr8_3:24249435-24249454 None:intergenic 25.0%
! TTTCTTTCATCTTATTAAGC+TGG - chr8_3:24250147-24250166 None:intergenic 25.0%
! AAGATGAAAGAAAACATTCT+TGG + chr8_3:24250154-24250173 Msa1283050:CDS 25.0%
TTAAGAGAGGTCAATATGAT+GGG - chr8_3:24247411-24247430 None:intergenic 30.0%
TTTAAGAGAGGTCAATATGA+TGG - chr8_3:24247412-24247431 None:intergenic 30.0%
TTAACAGAGCTTCGTATAAT+CGG + chr8_3:24247498-24247517 Msa1283050:CDS 30.0%
! GAAGATGGCAATTCTTTTAT+AGG - chr8_3:24247528-24247547 None:intergenic 30.0%
ATCATCTGATAAATTGCAGT+AGG - chr8_3:24247745-24247764 None:intergenic 30.0%
!! GAAAGTGTTTCTAATTTGCA+TGG - chr8_3:24247924-24247943 None:intergenic 30.0%
TGTAAGGTCTATGTATTTCA+AGG - chr8_3:24247964-24247983 None:intergenic 30.0%
AAGATTGACATTCTTCTGTA+AGG - chr8_3:24247980-24247999 None:intergenic 30.0%
! ATCTTTGAGAGAACTTATTC+TGG + chr8_3:24247995-24248014 Msa1283050:CDS 30.0%
TTTATCTTCGAGAAATTGAC+AGG + chr8_3:24248027-24248046 Msa1283050:CDS 30.0%
TTATCTTCGAGAAATTGACA+GGG + chr8_3:24248028-24248047 Msa1283050:CDS 30.0%
! AGTCAATAAAGTGCAGTTTT+TGG - chr8_3:24248081-24248100 None:intergenic 30.0%
! AGTTGATGCTATAATGTATC+AGG + chr8_3:24248226-24248245 Msa1283050:intron 30.0%
! CAAAGAAAACATCTTGTTTC+CGG - chr8_3:24248522-24248541 None:intergenic 30.0%
ATATTGAATCTCCACTTGTA+CGG - chr8_3:24248577-24248596 None:intergenic 30.0%
GCTAAAATTTGAAGATAACG+GGG + chr8_3:24248787-24248806 Msa1283050:CDS 30.0%
AGGATCTTATTACCAACAAT+GGG + chr8_3:24248858-24248877 Msa1283050:CDS 30.0%
AAAGTGGTTGCCAAATATAA+TGG + chr8_3:24248890-24248909 Msa1283050:CDS 30.0%
TACTACTTTGTTGAGATACA+TGG - chr8_3:24248919-24248938 None:intergenic 30.0%
! ACATTTACCAATGTTTGTTG+GGG - chr8_3:24248965-24248984 None:intergenic 30.0%
CACATTTACCAATGTTTGTT+GGG - chr8_3:24248966-24248985 None:intergenic 30.0%
TCACATTTACCAATGTTTGT+TGG - chr8_3:24248967-24248986 None:intergenic 30.0%
GATGTATAACAGGATTAACA+GGG - chr8_3:24249057-24249076 None:intergenic 30.0%
TGATGTATAACAGGATTAAC+AGG - chr8_3:24249058-24249077 None:intergenic 30.0%
!!! ATGCCTTTTTTGATTTCAGA+GGG - chr8_3:24249197-24249216 None:intergenic 30.0%
!!! AATGCCTTTTTTGATTTCAG+AGG - chr8_3:24249198-24249217 None:intergenic 30.0%
AGTAGCATAAGCTTCATTAT+CGG - chr8_3:24249291-24249310 None:intergenic 30.0%
AATAGAAAACTATGCGTTAG+TGG + chr8_3:24249446-24249465 Msa1283050:CDS 30.0%
TAGAGAGACTATTAATTCAC+TGG + chr8_3:24249523-24249542 Msa1283050:CDS 30.0%
! ACTGGAGTTATTGATGATTA+AGG + chr8_3:24249541-24249560 Msa1283050:CDS 30.0%
GAATTCATGAAATGATGCAA+AGG - chr8_3:24249699-24249718 None:intergenic 30.0%
GAAGAATTATGAGAATCATC+TGG + chr8_3:24249748-24249767 Msa1283050:CDS 30.0%
AAAGTTGAGCTGTTTAAGAG+AGG - chr8_3:24247424-24247443 None:intergenic 35.0%
GAGAGTTTCCCAGAAATATT+GGG + chr8_3:24247462-24247481 Msa1283050:five_prime_UTR 35.0%
AGAGTTTCCCAGAAATATTG+GGG + chr8_3:24247463-24247482 Msa1283050:five_prime_UTR 35.0%
! CTAATTTTCGAAGCCGATTA+AGG - chr8_3:24247560-24247579 None:intergenic 35.0%
AGAACTACAAATCTGTGGAA+TGG + chr8_3:24247578-24247597 Msa1283050:CDS 35.0%
! ATTGCCATGTTTTCACAACT+TGG + chr8_3:24247618-24247637 Msa1283050:CDS 35.0%
CAAACCAAGTTGTGAAAACA+TGG - chr8_3:24247625-24247644 None:intergenic 35.0%
AAATTCTGTCTTCGAAGACT+TGG - chr8_3:24247712-24247731 None:intergenic 35.0%
GTTTGCCAATGTGAAAGATT+TGG + chr8_3:24247788-24247807 Msa1283050:CDS 35.0%
TAGGTCCAAATCTTTCACAT+TGG - chr8_3:24247796-24247815 None:intergenic 35.0%
TAGTGAAATTATTCCCGGAT+AGG - chr8_3:24247815-24247834 None:intergenic 35.0%
CTATAATGTATCAGGCAGAT+TGG + chr8_3:24248234-24248253 Msa1283050:intron 35.0%
TTTCTAAACTTATCACCGAC+AGG + chr8_3:24248484-24248503 Msa1283050:intron 35.0%
! AACAAGATGTTTTCTTTGCC+AGG + chr8_3:24248524-24248543 Msa1283050:CDS 35.0%
GTGGAGATTCAATATCTTTC+TGG + chr8_3:24248582-24248601 Msa1283050:CDS 35.0%
! ATTTGTCTGTGCCTTGTTAT+TGG + chr8_3:24248626-24248645 Msa1283050:CDS 35.0%
!! TTTGTCTGTGCCTTGTTATT+GGG + chr8_3:24248627-24248646 Msa1283050:CDS 35.0%
! TAGTGTTTTCAGACAATGAG+TGG + chr8_3:24248813-24248832 Msa1283050:CDS 35.0%
GAGGATCTTATTACCAACAA+TGG + chr8_3:24248857-24248876 Msa1283050:CDS 35.0%
GGATCTTATTACCAACAATG+GGG + chr8_3:24248859-24248878 Msa1283050:CDS 35.0%
TATTTGGCAACCACTTTGAT+TGG - chr8_3:24248887-24248906 None:intergenic 35.0%
TTTCAATCCTGATTCAATCG+TGG + chr8_3:24248988-24249007 Msa1283050:CDS 35.0%
TTCAATCCTGATTCAATCGT+GGG + chr8_3:24248989-24249008 Msa1283050:CDS 35.0%
ATTTCCAGTAAAGATGCCTT+TGG + chr8_3:24249232-24249251 Msa1283050:CDS 35.0%
TTTCCAGTAAAGATGCCTTT+GGG + chr8_3:24249233-24249252 Msa1283050:CDS 35.0%
TCTCTACATCTTTGAATGAG+AGG - chr8_3:24249332-24249351 None:intergenic 35.0%
TCTCATTCAAAGATGTAGAG+AGG + chr8_3:24249331-24249350 Msa1283050:CDS 35.0%
CTCATTCAAAGATGTAGAGA+GGG + chr8_3:24249332-24249351 Msa1283050:CDS 35.0%
GCAAGAAAACCATATCTTCT+GGG + chr8_3:24249468-24249487 Msa1283050:CDS 35.0%
CAAGAAAACCATATCTTCTG+GGG + chr8_3:24249469-24249488 Msa1283050:CDS 35.0%
TTCATCTCTATTGTCACCTT+GGG - chr8_3:24249647-24249666 None:intergenic 35.0%
TATTGCTGAGTTCAAGAGAA+AGG + chr8_3:24249673-24249692 Msa1283050:CDS 35.0%
GAAGGTTTCTTTGTACATCA+AGG + chr8_3:24249835-24249854 Msa1283050:CDS 35.0%
ACATCAAGGCTGAAAATAGT+AGG + chr8_3:24249849-24249868 Msa1283050:CDS 35.0%
GGCTGAAAATAGTAGGAAAA+AGG + chr8_3:24249856-24249875 Msa1283050:CDS 35.0%
TGTGTTGAATCTCAAGAATC+AGG + chr8_3:24249946-24249965 Msa1283050:CDS 35.0%
TTTCATCTTATTAAGCTGGC+CGG - chr8_3:24250143-24250162 None:intergenic 35.0%
CGAGAGTTTCCCAGAAATAT+TGG + chr8_3:24247461-24247480 Msa1283050:five_prime_UTR 40.0%
CCATCTTCCCCAATATTTCT+GGG - chr8_3:24247473-24247492 None:intergenic 40.0%
TCCATCTTCCCCAATATTTC+TGG - chr8_3:24247474-24247493 None:intergenic 40.0%
GAAAACATGGCAATGCTACT+TGG - chr8_3:24247612-24247631 None:intergenic 40.0%
ATGTGTGTTTCGAGATGTGA+AGG + chr8_3:24247645-24247664 Msa1283050:CDS 40.0%
TGTGTGTTTCGAGATGTGAA+GGG + chr8_3:24247646-24247665 Msa1283050:CDS 40.0%
AGGGTTGTGGTTATCTAAAC+CGG + chr8_3:24247665-24247684 Msa1283050:CDS 40.0%
AACCGGACATAAGTGAAGAA+TGG + chr8_3:24247682-24247701 Msa1283050:CDS 40.0%
ACCGGACATAAGTGAAGAAT+GGG + chr8_3:24247683-24247702 Msa1283050:CDS 40.0%
TCCCATTCTTCACTTATGTC+CGG - chr8_3:24247687-24247706 None:intergenic 40.0%
TCAGATGATTTCCTTCCCAT+AGG + chr8_3:24247756-24247775 Msa1283050:CDS 40.0%
GTGAAAGATTTGGACCTATC+CGG + chr8_3:24247798-24247817 Msa1283050:CDS 40.0%
TGAAAGATTTGGACCTATCC+GGG + chr8_3:24247799-24247818 Msa1283050:CDS 40.0%
!! TCAAAGAGTGTCACTTTCTC+AGG + chr8_3:24247850-24247869 Msa1283050:CDS 40.0%
GCAGAGAAATGATCCAAGTT+TGG - chr8_3:24248059-24248078 None:intergenic 40.0%
!! TGCAAGCTTGTTAGTGAATC+AGG + chr8_3:24248109-24248128 Msa1283050:CDS 40.0%
!!! GATTGGTTGATGGAGTGTTT+TGG + chr8_3:24248251-24248270 Msa1283050:intron 40.0%
TTGCCATTGAGGAACACTTT+GGG - chr8_3:24248689-24248708 None:intergenic 40.0%
ATCAGGTAGTCTACAAGTTC+AGG + chr8_3:24248726-24248745 Msa1283050:CDS 40.0%
AGACAATGAGTGGAATCATG+TGG + chr8_3:24248823-24248842 Msa1283050:CDS 40.0%
TCATGTGGTGGTTTCATATG+AGG + chr8_3:24248838-24248857 Msa1283050:CDS 40.0%
TTTGATTGGAACCCCATTGT+TGG - chr8_3:24248873-24248892 None:intergenic 40.0%
TTTGTTGGGGATTGCTGAAT+CGG - chr8_3:24248952-24248971 None:intergenic 40.0%
TCAATCCTGATTCAATCGTG+GGG + chr8_3:24248990-24249009 Msa1283050:CDS 40.0%
GAGCATGAGTGATGTATAAC+AGG - chr8_3:24249067-24249086 None:intergenic 40.0%
! GATTGTGTTGATGTGAGTGA+GGG - chr8_3:24249164-24249183 None:intergenic 40.0%
! AGATTGTGTTGATGTGAGTG+AGG - chr8_3:24249165-24249184 None:intergenic 40.0%
!!! CCTTTTTTGATTTCAGAGGG+TGG - chr8_3:24249194-24249213 None:intergenic 40.0%
!! CCACCCTCTGAAATCAAAAA+AGG + chr8_3:24249191-24249210 Msa1283050:CDS 40.0%
GGCATCTTTACTGGAAATGT+AGG - chr8_3:24249230-24249249 None:intergenic 40.0%
GGCAAGAAAACCATATCTTC+TGG + chr8_3:24249467-24249486 Msa1283050:CDS 40.0%
AGAACTTACCCCAGAAGATA+TGG - chr8_3:24249480-24249499 None:intergenic 40.0%
!! ATATCTTCTGGGGTAAGTTC+TGG + chr8_3:24249479-24249498 Msa1283050:CDS 40.0%
TCATCTCTATTGTCACCTTG+GGG - chr8_3:24249646-24249665 None:intergenic 40.0%
CTTCATCTCTATTGTCACCT+TGG - chr8_3:24249648-24249667 None:intergenic 40.0%
CAAGGTGACAATAGAGATGA+AGG + chr8_3:24249646-24249665 Msa1283050:CDS 40.0%
CTTACTCACAGATTCAACAG+TGG - chr8_3:24249726-24249745 None:intergenic 40.0%
! ACAGCTAGCTTCCAAAGAAA+AGG + chr8_3:24249910-24249929 Msa1283050:CDS 40.0%
! TTGTTCGAGGTCCTTTTCTT+TGG - chr8_3:24249924-24249943 None:intergenic 40.0%
GATTCAACACAAGTTGTTCG+AGG - chr8_3:24249937-24249956 None:intergenic 40.0%
GATGATCTACTTGCTTCAAG+CGG + chr8_3:24250043-24250062 Msa1283050:CDS 40.0%
AGCGGTGAGATAAAACATGA+TGG + chr8_3:24250061-24250080 Msa1283050:CDS 40.0%
ACTTGGTCTATTGACCTAAC+CGG + chr8_3:24250121-24250140 Msa1283050:CDS 40.0%
! AGAGAGGTCAATATGATGGG+TGG - chr8_3:24247408-24247427 None:intergenic 45.0%
CCCAGAAATATTGGGGAAGA+TGG + chr8_3:24247470-24247489 Msa1283050:exon 45.0%
TTTCGAGATGTGAAGGGTTG+TGG + chr8_3:24247652-24247671 Msa1283050:CDS 45.0%
GCAAACCAAGACAGACCTAT+GGG - chr8_3:24247774-24247793 None:intergenic 45.0%
GCACGCAGTCATCTATCTTT+AGG - chr8_3:24247878-24247897 None:intergenic 45.0%
! GTATCAGGCAGATTGGTTGA+TGG + chr8_3:24248241-24248260 Msa1283050:intron 45.0%
CGGCTTCAATCATTTCCTGT+CGG - chr8_3:24248502-24248521 None:intergenic 45.0%
CGACAGGAAATGATTGAAGC+CGG + chr8_3:24248500-24248519 Msa1283050:intron 45.0%
!! CACTCTGGAATCTTTGTTCC+TGG - chr8_3:24248545-24248564 None:intergenic 45.0%
CAGGAACAAAGATTCCAGAG+TGG + chr8_3:24248543-24248562 Msa1283050:CDS 45.0%
! TGGTTTTCACACCGTACAAG+TGG + chr8_3:24248563-24248582 Msa1283050:CDS 45.0%
CAAGGCACAGACAAATTGCA+GGG - chr8_3:24248622-24248641 None:intergenic 45.0%
ACAAGGCACAGACAAATTGC+AGG - chr8_3:24248623-24248642 None:intergenic 45.0%
GGGCTAAACTTGACTGAGAT+AGG - chr8_3:24248668-24248687 None:intergenic 45.0%
TGCCATTGAGGAACACTTTG+GGG - chr8_3:24248688-24248707 None:intergenic 45.0%
GTTGCCATTGAGGAACACTT+TGG - chr8_3:24248690-24248709 None:intergenic 45.0%
CAATGAGTGGAATCATGTGG+TGG + chr8_3:24248826-24248845 Msa1283050:CDS 45.0%
CAATGGGGTTCCAATCAAAG+TGG + chr8_3:24248874-24248893 Msa1283050:CDS 45.0%
CAGCAATCCCCAACAAACAT+TGG + chr8_3:24248955-24248974 Msa1283050:CDS 45.0%
GATTATGACTAAGAGACCCC+AGG + chr8_3:24249115-24249134 Msa1283050:intron 45.0%
TTGATTTCAGAGGGTGGAGA+AGG - chr8_3:24249188-24249207 None:intergenic 45.0%
!! TCTCCCAAAGGCATCTTTAC+TGG - chr8_3:24249239-24249258 None:intergenic 45.0%
! AAAGATGCCTTTGGGAGATG+TGG + chr8_3:24249241-24249260 Msa1283050:CDS 45.0%
! AAGATGCCTTTGGGAGATGT+GGG + chr8_3:24249242-24249261 Msa1283050:CDS 45.0%
GGAAGAAGACTCTTCTTCAC+AGG - chr8_3:24249384-24249403 None:intergenic 45.0%
ATCATCCGAGTCAGAACCTT+CGG - chr8_3:24249411-24249430 None:intergenic 45.0%
! CTGACGAAGTTTGGAATGTG+CGG - chr8_3:24249594-24249613 None:intergenic 45.0%
TTCCAAACTTCGTCAGCTCT+TGG + chr8_3:24249598-24249617 Msa1283050:CDS 45.0%
ATCCAAGAGCTGACGAAGTT+TGG - chr8_3:24249603-24249622 None:intergenic 45.0%
GGTGACAATAGAGATGAAGG+AGG + chr8_3:24249649-24249668 Msa1283050:CDS 45.0%
TCACAGATTCAACAGTGGCT+TGG - chr8_3:24249721-24249740 None:intergenic 45.0%
CAGCTGAAATAGAGCAGAGT+AGG + chr8_3:24250089-24250108 Msa1283050:CDS 45.0%
TCTTATTAAGCTGGCCGGTT+AGG - chr8_3:24250138-24250157 None:intergenic 45.0%
TCCTTCCCATAGGTCTGTCT+TGG + chr8_3:24247766-24247785 Msa1283050:CDS 50.0%
ACCAAGACAGACCTATGGGA+AGG - chr8_3:24247770-24247789 None:intergenic 50.0%
GGCAAACCAAGACAGACCTA+TGG - chr8_3:24247775-24247794 None:intergenic 50.0%
TGACAGGGTTCTGCCAAACT+TGG + chr8_3:24248043-24248062 Msa1283050:CDS 50.0%
GTACGGTGTGAAAACCACTC+TGG - chr8_3:24248560-24248579 None:intergenic 50.0%
!! TCTGTGCCTTGTTATTGGGC+AGG + chr8_3:24248631-24248650 Msa1283050:CDS 50.0%
! AGCCCCAAAGTGTTCCTCAA+TGG + chr8_3:24248683-24248702 Msa1283050:CDS 50.0%
TGGACTGTTCGTTGCCATTG+AGG - chr8_3:24248700-24248719 None:intergenic 50.0%
AATGGCAACGAACAGTCCAG+TGG + chr8_3:24248701-24248720 Msa1283050:CDS 50.0%
GTAGACTACCTGATTGCCAC+TGG - chr8_3:24248720-24248739 None:intergenic 50.0%
GGGGTCACCTCAAAGAGTAA+AGG + chr8_3:24249009-24249028 Msa1283050:CDS 50.0%
!! ACAGTGGTTTGGTCTTCCTG+GGG - chr8_3:24249134-24249153 None:intergenic 50.0%
!! CACAGTGGTTTGGTCTTCCT+GGG - chr8_3:24249135-24249154 None:intergenic 50.0%
TCTCTTCCCACATCTCCCAA+AGG - chr8_3:24249251-24249270 None:intergenic 50.0%
GAGTCTTCTTCCAACACCGA+AGG + chr8_3:24249392-24249411 Msa1283050:CDS 50.0%
! CAACACCGAAGGTTCTGACT+CGG + chr8_3:24249403-24249422 Msa1283050:CDS 50.0%
! CGAAGTTTGGAATGTGCGGA+AGG - chr8_3:24249590-24249609 None:intergenic 50.0%
TCTCTATTGTCACCTTGGGG+TGG - chr8_3:24249643-24249662 None:intergenic 50.0%
CACAGTCTCGTGCTGAATTC+TGG - chr8_3:24249774-24249793 None:intergenic 50.0%
CAGAATTCAGCACGAGACTG+TGG + chr8_3:24249772-24249791 Msa1283050:CDS 50.0%
GTCCTTCCATTGATCCTTGC+CGG - chr8_3:24249798-24249817 None:intergenic 50.0%
TAGAGCAGAGTAGGCTCAAG+TGG + chr8_3:24250098-24250117 Msa1283050:CDS 50.0%
! AGAGTAGGCTCAAGTGGACT+TGG + chr8_3:24250104-24250123 Msa1283050:CDS 50.0%
!! GTGCCTTGTTATTGGGCAGG+CGG + chr8_3:24248634-24248653 Msa1283050:CDS 55.0%
CATCCGCCTGCCCAATAACA+AGG - chr8_3:24248640-24248659 None:intergenic 55.0%
CGAACAGTCCAGTGGCAATC+AGG + chr8_3:24248709-24248728 Msa1283050:CDS 55.0%
GGTGACCCCACGATTGAATC+AGG - chr8_3:24248998-24249017 None:intergenic 55.0%
!! GCACAGTGGTTTGGTCTTCC+TGG - chr8_3:24249136-24249155 None:intergenic 55.0%
AGGGAGACAGCACAGTGGTT+TGG - chr8_3:24249145-24249164 None:intergenic 55.0%
! AGAGGGTGATGATGTCGAGC+TGG + chr8_3:24249349-24249368 Msa1283050:CDS 55.0%
! CGAGTCAGAACCTTCGGTGT+TGG - chr8_3:24249405-24249424 None:intergenic 55.0%
ATTCAGCACGAGACTGTGGC+CGG + chr8_3:24249776-24249795 Msa1283050:CDS 55.0%
GAGTGAGGGAGACAGCACAG+TGG - chr8_3:24249150-24249169 None:intergenic 60.0%
GAGTGCAAGTAGCCACCCCA+AGG + chr8_3:24249628-24249647 Msa1283050:CDS 60.0%
CTGTGGCCGGCAAGGATCAA+TGG + chr8_3:24249789-24249808 Msa1283050:CDS 60.0%
GGCCGGCAAGGATCAATGGA+AGG + chr8_3:24249793-24249812 Msa1283050:CDS 60.0%
GCACGAGACTGTGGCCGGCA+AGG + chr8_3:24249781-24249800 Msa1283050:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 24247392 24250189 24247392 ID=Msa1283050;Name=Msa1283050
chr8_3 mRNA 24247392 24250189 24247392 ID=Msa1283050-mRNA-1;Parent=Msa1283050;Name=Msa1283050-mRNA-1;_AED=0.34;_eAED=0.34;_QI=97|1|0.66|1|1|0.66|3|0|746
chr8_3 exon 24247392 24248130 24247392 ID=Msa1283050-mRNA-1:exon:8577;Parent=Msa1283050-mRNA-1
chr8_3 exon 24248506 24249030 24248506 ID=Msa1283050-mRNA-1:exon:8578;Parent=Msa1283050-mRNA-1
chr8_3 exon 24249116 24250189 24249116 ID=Msa1283050-mRNA-1:exon:8579;Parent=Msa1283050-mRNA-1
chr8_3 five_prime_UTR 24247392 24247488 24247392 ID=Msa1283050-mRNA-1:five_prime_utr;Parent=Msa1283050-mRNA-1
chr8_3 CDS 24247489 24248130 24247489 ID=Msa1283050-mRNA-1:cds;Parent=Msa1283050-mRNA-1
chr8_3 CDS 24248506 24249030 24248506 ID=Msa1283050-mRNA-1:cds;Parent=Msa1283050-mRNA-1
chr8_3 CDS 24249116 24250189 24249116 ID=Msa1283050-mRNA-1:cds;Parent=Msa1283050-mRNA-1
Gene Sequence

>Msa1283050

ATGGAAAACTTAACAGAGCTTCGTATAATCGGAAGTCCTATAAAAGAATTGCCATCTTCAATTCAAAACCTTAATCGGCTTCGAAAATTAGAACTACAAATCTGTGGAATGGTTCAGTTACCAAGTAGCATTGCCATGTTTTCACAACTTGGTTTGATGTGTGTTTCGAGATGTGAAGGGTTGTGGTTATCTAAACCGGACATAAGTGAAGAATGGGAGACCAAGTCTTCGAAGACAGAATTTCTTATTCTGTCCTACTGCAATTTATCAGATGATTTCCTTCCCATAGGTCTGTCTTGGTTTGCCAATGTGAAAGATTTGGACCTATCCGGGAATAATTTCACTATTCTTCACGCATGCATCAAAGAGTGTCACTTTCTCAGGAACCTAAAGATAGATGACTGCGTGCATATACGAGAAATTACAGTGATACCATGCAAATTAGAAACACTTTCTGCAAAACGATGCAAATCCTTGAAATACATAGACCTTACAGAAGAATGTCAATCTTTGAGAGAACTTATTCTGGACGACTGTATTTATCTTCGAGAAATTGACAGGGTTCTGCCAAACTTGGATCATTTCTCTGCCAAAAACTGCACTTTATTGACTTCTCAGTGTGCAAGCTTGTTAGTGAATCAGGAAATGATTGAAGCCGGAAACAAGATGTTTTCTTTGCCAGGAACAAAGATTCCAGAGTGGTTTTCACACCGTACAAGTGGAGATTCAATATCTTTCTGGTTTCGTAACAGATTCCCTGCAATTTGTCTGTGCCTTGTTATTGGGCAGGCGGATGAGCTACCTATCTCAGTCAAGTTTAGCCCCAAAGTGTTCCTCAATGGCAACGAACAGTCCAGTGGCAATCAGGTAGTCTACAAGTTCAGGATAGCTACTGATCATATACTTTTGTTTGATGGGCAGATGCTAAAATTTGAAGATAACGGGGAAATAGTGTTTTCAGACAATGAGTGGAATCATGTGGTGGTTTCATATGAGGATCTTATTACCAACAATGGGGTTCCAATCAAAGTGGTTGCCAAATATAATGGAATCCATGTATCTCAACAAAGTAGTATTGAGAATATCCGATTCAGCAATCCCCAACAAACATTGGTAAATGTGAATTTCAATCCTGATTCAATCGTGGGGTCACCTCAAAGAGTAAAGATTATGACTAAGAGACCCCAGGAAGACCAAACCACTGTGCTGTCTCCCTCACTCACATCAACACAATCTCCTTCTCCACCCTCTGAAATCAAAAAAGGCATTGTCACTCTTCCTACATTTCCAGTAAAGATGCCTTTGGGAGATGTGGGAAGAGAAAGTTCAGAGAATCTTTCCGATAATGAAGCTTATGCTACTGCAATTCAAAATGAGACTCCTCTCATTCAAAGATGTAGAGAGGGTGATGATGTCGAGCTGGAATTAGTATCCTGTGAAGAAGAGTCTTCTTCCAACACCGAAGGTTCTGACTCGGATGATCCCTTCGACTTAGTTAATAGAAAACTATGCGTTAGTGGCAAGAAAACCATATCTTCTGGGGTAAGTTCTGGAGATGCAAATCTAAGATCTATTAGAGAGACTATTAATTCACTGGAGTTATTGATGATTAAGGACTTATCTGAAGTCTCTGCTGATCCTTCCGCACATTCCAAACTTCGTCAGCTCTTGGATCTATTGAGTGCAAGTAGCCACCCCAAGGTGACAATAGAGATGAAGGAGGCTATTGCTGAGTTCAAGAGAAAGGCCTTTGCATCATTTCATGAATTCCAAGCCACTGTTGAATCTGTGAGTAAGCTGAAGAATTATGAGAATCATCTGGCCAGAATTCAGCACGAGACTGTGGCCGGCAAGGATCAATGGAAGGACTTCAAAAAATCAATAAAGAAGGTTTCTTTGTACATCAAGGCTGAAAATAGTAGGAAAAAGGAACTAGAGTCAGAAATTGCAACTTTAAGAAAACAGCTAGCTTCCAAAGAAAAGGACCTCGAACAACTTGTGTTGAATCTCAAGAATCAGGAGACAACACTCTCAACTTATTCAACGAGTTATGCTTCTCTCAATGAACATGCTCGAGCACTATCAGAAGAAGCTGATGATCTACTTGCTTCAAGCGGTGAGATAAAACATGATGGCGAAGCAGCTGAAATAGAGCAGAGTAGGCTCAAGTGGACTTGGTCTATTGACCTAACCGGCCAGCTTAATAAGATGAAAGAAAACATTCTTGGCTCATATGAGTAG
Protein sequence

>Msa1283050

MENLTELRIIGSPIKELPSSIQNLNRLRKLELQICGMVQLPSSIAMFSQLGLMCVSRCEGLWLSKPDISEEWETKSSKTEFLILSYCNLSDDFLPIGLSWFANVKDLDLSGNNFTILHACIKECHFLRNLKIDDCVHIREITVIPCKLETLSAKRCKSLKYIDLTEECQSLRELILDDCIYLREIDRVLPNLDHFSAKNCTLLTSQCASLLVNQEMIEAGNKMFSLPGTKIPEWFSHRTSGDSISFWFRNRFPAICLCLVIGQADELPISVKFSPKVFLNGNEQSSGNQVVYKFRIATDHILLFDGQMLKFEDNGEIVFSDNEWNHVVVSYEDLITNNGVPIKVVAKYNGIHVSQQSSIENIRFSNPQQTLVNVNFNPDSIVGSPQRVKIMTKRPQEDQTTVLSPSLTSTQSPSPPSEIKKGIVTLPTFPVKMPLGDVGRESSENLSDNEAYATAIQNETPLIQRCREGDDVELELVSCEEESSSNTEGSDSDDPFDLVNRKLCVSGKKTISSGVSSGDANLRSIRETINSLELLMIKDLSEVSADPSAHSKLRQLLDLLSASSHPKVTIEMKEAIAEFKRKAFASFHEFQATVESVSKLKNYENHLARIQHETVAGKDQWKDFKKSIKKVSLYIKAENSRKKELESEIATLRKQLASKEKDLEQLVLNLKNQETTLSTYSTSYASLNEHARALSEEADDLLASSGEIKHDGEAAEIEQSRLKWTWSIDLTGQLNKMKENILGSYE*