AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1187720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1187720 MtrunA17_Chr8g0354711 84.820 751 107 5 1 745 717 1466 0.0 1273
Msa1187720 MtrunA17_Chr8g0354701 68.809 747 231 2 1 746 722 1467 0.0 1030
Msa1187720 MtrunA17_Chr8g0354721 54.557 790 294 11 1 744 721 1491 0.0 762
Msa1187720 MtrunA17_Chr8g0354681 60.299 335 120 4 1 329 725 1052 9.06e-115 371
Msa1187720 MtrunA17_Chr8g0354671 49.223 386 147 4 402 743 2 382 2.57e-103 322
Msa1187720 MtrunA17_Chr8g0353371 45.263 380 190 7 1 367 733 1107 5.22e-83 286
Msa1187720 MtrunA17_Chr8g0371711 37.903 372 170 9 1 367 724 1039 2.31e-62 226
Msa1187720 MtrunA17_Chr5g0415831 36.243 378 175 9 1 367 91 413 1.72e-54 193
Msa1187720 MtrunA17_Chr8g0379351 37.377 305 140 3 2 304 250 505 2.99e-51 186
Msa1187720 MtrunA17_Chr6g0472561 34.817 382 181 11 1 371 1 325 7.42e-51 181
Msa1187720 MtrunA17_Chr6g0480981 34.865 370 178 12 3 367 463 774 3.19e-50 188
Msa1187720 MtrunA17_Chr6g0481011 35.356 379 175 11 1 371 735 1051 2.92e-48 184
Msa1187720 MtrunA17_Chr6g0482141 33.248 391 181 10 1 382 893 1212 2.92e-46 178
Msa1187720 MtrunA17_Chr6g0482511 34.545 385 173 11 1 367 749 1072 1.83e-45 176
Msa1187720 MtrunA17_Chr3g0077971 34.133 375 164 10 1 368 1260 1558 2.16e-45 176
Msa1187720 MtrunA17_Chr6g0482461 33.777 376 169 13 1 368 374 677 1.39e-43 167
Msa1187720 MtrunA17_Chr3g0084151 32.723 382 189 8 1 365 703 1033 5.88e-43 168
Msa1187720 MtrunA17_Chr3g0081531 23.885 762 458 19 1 727 709 1383 7.56e-43 168
Msa1187720 MtrunA17_Chr6g0482491 31.367 373 190 6 1 367 964 1276 1.46e-42 167
Msa1187720 MtrunA17_Chr1g0172541 31.152 382 182 10 1 376 836 1142 2.60e-42 166
Msa1187720 MtrunA17_Chr6g0480911 33.245 379 188 9 1 369 731 1054 2.70e-40 159
Msa1187720 MtrunA17_Chr6g0482351 32.258 372 179 12 1 368 1176 1478 2.32e-39 157
Msa1187720 MtrunA17_Chr8g0350801 35.762 302 125 9 75 367 836 1077 2.44e-39 157
Msa1187720 MtrunA17_Chr6g0482321 40.234 256 124 10 127 372 913 1149 3.06e-39 156
Msa1187720 MtrunA17_Chr6g0482441 31.818 374 173 13 1 368 978 1275 1.11e-37 152
Msa1187720 MtrunA17_Chr8g0353381 42.945 163 83 5 210 367 1 158 3.47e-36 134
Msa1187720 MtrunA17_Chr6g0451551 29.708 377 184 11 1 368 867 1171 3.96e-36 147
Msa1187720 MtrunA17_Chr4g0006771 35.069 288 135 4 1 282 707 948 2.64e-35 144
Msa1187720 MtrunA17_Chr2g0300251 31.358 405 204 13 1 390 711 1056 2.39e-33 138
Msa1187720 MtrunA17_Chr6g0479631 28.571 357 193 9 14 358 697 1003 6.91e-32 133
Msa1187720 MtrunA17_Chr6g0482111 29.333 375 201 8 1 367 719 1037 1.73e-31 132
Msa1187720 MtrunA17_Chr6g0481231 29.487 390 183 11 1 373 101 415 3.88e-31 127
Msa1187720 MtrunA17_Chr6g0481251 28.643 398 184 13 1 377 565 883 1.28e-30 129
Msa1187720 MtrunA17_Chr6g0481141 26.448 397 196 9 1 377 758 1078 1.17e-29 126
Msa1187720 MtrunA17_Chr6g0481001 34.615 208 113 9 175 371 775 970 1.44e-29 126
Msa1187720 MtrunA17_Chr6g0484161 29.412 391 177 13 1 369 727 1040 1.58e-29 125
Msa1187720 MtrunA17_Chr6g0480051 30.851 376 188 11 1 366 483 796 1.27e-28 122
Msa1187720 MtrunA17_Chr6g0480041 29.262 393 183 13 1 373 723 1040 1.37e-28 123
Msa1187720 MtrunA17_Chr6g0479591 29.723 397 185 15 19 373 651 995 4.79e-28 121
Msa1187720 MtrunA17_Chr6g0479841 29.674 337 173 10 14 337 350 635 5.56e-28 120
Msa1187720 MtrunA17_Chr6g0479001 27.416 445 224 15 1 420 691 1061 3.82e-27 118
Msa1187720 MtrunA17_Chr1g0153511 50.769 130 59 2 1 127 1284 1411 6.98e-27 117
Msa1187720 MtrunA17_Chr6g0480421 30.423 378 162 15 1 367 566 853 3.64e-26 115
Msa1187720 MtrunA17_Chr6g0478451 30.208 384 180 13 1 367 710 1022 6.07e-26 114
Msa1187720 MtrunA17_Chr6g0480451 29.925 401 196 20 1 381 743 1078 1.12e-25 114
Msa1187720 MtrunA17_Chr6g0479741 29.560 318 145 13 74 367 35 297 1.84e-25 107
Msa1187720 MtrunA17_Chr6g0479671 30.025 403 198 16 1 380 706 1047 2.43e-25 112
Msa1187720 MtrunA17_Chr6g0479621 28.465 404 177 15 1 367 703 1031 6.82e-25 111
Msa1187720 MtrunA17_Chr6g0479641 29.639 388 171 14 1 367 709 1015 6.92e-25 111
Msa1187720 MtrunA17_Chr6g0480321 29.737 380 176 16 1 367 562 863 1.56e-24 109
Msa1187720 MtrunA17_Chr6g0480401 32.540 252 119 6 1 247 178 383 1.87e-24 106
Msa1187720 MtrunA17_Chr6g0478841 29.189 370 193 13 14 369 682 996 2.07e-24 109
Msa1187720 MtrunA17_Chr4g0025831 25.858 379 188 12 1 367 704 1001 1.29e-23 107
Msa1187720 MtrunA17_Chr6g0479051 26.997 363 174 10 1 353 719 1000 2.10e-23 106
Msa1187720 MtrunA17_Chr1g0153501 37.654 162 79 8 212 368 1 145 7.00e-23 95.9
Msa1187720 MtrunA17_Chr6g0479821 29.878 328 168 17 19 327 639 923 1.47e-22 103
Msa1187720 MtrunA17_Chr5g0417051 31.873 251 124 7 1 248 75 281 1.52e-22 98.6
Msa1187720 MtrunA17_Chr6g0480521 27.128 376 182 14 1 367 705 997 1.62e-22 103
Msa1187720 MtrunA17_Chr6g0480861 40.449 178 94 3 1 177 1 167 2.27e-22 95.1
Msa1187720 MtrunA17_Chr6g0478771 29.644 253 104 5 1 247 692 876 4.56e-22 102
Msa1187720 MtrunA17_Chr1g0181021 27.134 328 158 9 74 369 27 305 5.54e-22 97.4
Msa1187720 MtrunA17_Chr6g0479651 27.344 384 201 10 1 366 715 1038 6.06e-22 101
Msa1187720 MtrunA17_Chr1g0146421 26.893 383 189 12 1 367 701 1008 1.05e-21 100
Msa1187720 MtrunA17_Chr6g0479701 28.531 354 176 13 3 327 623 928 1.49e-21 100
Msa1187720 MtrunA17_Chr5g0417071 28.814 295 140 9 84 367 308 543 2.85e-21 98.6
Msa1187720 MtrunA17_Chr4g0025771 26.250 400 227 17 19 367 87 469 4.37e-21 97.8
Msa1187720 MtrunA17_Chr5g0442761 26.684 386 224 12 1 367 718 1063 9.44e-21 98.2
Msa1187720 MtrunA17_Chr8g0368251 26.753 385 183 14 1 367 703 1006 3.13e-20 96.3
Msa1187720 MtrunA17_Chr6g0480311 27.841 352 168 11 16 354 709 987 1.09e-19 94.7
Msa1187720 MtrunA17_Chr4g0010521 26.131 398 205 14 1 369 710 1047 7.54e-19 92.0
Msa1187720 MtrunA17_Chr4g0025811 24.439 401 229 14 19 367 625 1003 8.21e-19 91.7
Msa1187720 MtrunA17_Chr4g0025691 28.622 283 155 13 101 367 40 291 1.30e-18 88.2
Msa1187720 MtrunA17_Chr4g0010651 25.952 420 215 17 1 387 712 1068 3.12e-18 90.1
Msa1187720 MtrunA17_Chr6g0479761 36.943 157 74 2 1 156 565 697 3.29e-17 86.3
Msa1187720 MtrunA17_Chr6g0478741 34.756 164 76 2 1 159 671 808 1.44e-16 84.3
Msa1187720 MtrunA17_Chr6g0480361 35.047 214 111 13 167 367 247 445 3.01e-16 82.0
Msa1187720 MtrunA17_Chr6g0480931 37.662 154 79 9 223 369 1 144 2.79e-15 74.3
Msa1187720 MtrunA17_Chr6g0480961 42.105 76 42 2 210 283 1 76 5.99e-15 70.9
Msa1187720 MtrunA17_Chr4g0025751 28.358 268 141 10 19 249 616 869 7.72e-15 79.0
Msa1187720 MtrunA17_Chr8g0363891 26.378 254 162 6 1 239 303 546 4.68e-13 72.8
Msa1187720 MtrunA17_Chr6g0465271 50.877 57 25 2 207 260 11 67 1.21e-12 63.9
Msa1187720 MtrunA17_Chr7g0248121 26.117 291 156 7 1 260 767 1029 1.29e-11 68.6
Msa1187720 MtrunA17_Chr4g0025741 32.857 140 89 2 14 153 692 826 1.68e-11 67.8
Msa1187720 MtrunA17_Chr8g0363851 28.195 266 152 8 1 246 767 1013 1.73e-11 68.2
Msa1187720 MtrunA17_Chr2g0299921 25.000 348 213 15 1 332 621 936 2.30e-11 67.8
Msa1187720 MtrunA17_Chr2g0281531 36.842 133 66 3 1 132 267 382 4.48e-11 65.5
Msa1187720 MtrunA17_Chr2g0299881 24.128 344 224 13 1 332 627 945 4.67e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1187720 AT1G27180.1 28.854 253 160 7 1 237 1148 1396 1.59e-14 78.6
Msa1187720 AT1G27180.5 28.854 253 160 7 1 237 984 1232 1.75e-14 78.2
Msa1187720 AT1G27180.8 28.854 253 160 7 1 237 984 1232 1.75e-14 78.2
Msa1187720 AT1G27180.2 28.854 253 160 7 1 237 986 1234 1.91e-14 78.2
Msa1187720 AT1G27180.7 28.854 253 160 7 1 237 810 1058 2.15e-14 77.8
Msa1187720 AT1G27180.6 28.854 253 160 7 1 237 810 1058 2.15e-14 77.8
Msa1187720 AT1G27180.4 28.854 253 160 7 1 237 810 1058 2.15e-14 77.8
Msa1187720 AT1G27170.1 28.063 253 162 7 1 237 974 1222 3.81e-13 73.9
Msa1187720 AT1G27170.2 28.063 253 162 7 1 237 974 1222 4.68e-13 73.6
Msa1187720 AT5G41740.1 25.574 305 193 12 16 309 650 931 4.04e-12 70.5
Msa1187720 AT5G41740.3 25.574 305 193 12 16 309 498 779 4.05e-12 70.5
Msa1187720 AT5G41740.2 25.574 305 193 12 16 309 650 931 4.94e-12 70.1
Msa1187720 AT5G17880.2 25.000 364 213 16 2 341 750 1077 8.78e-12 69.3
Msa1187720 AT5G17880.1 25.000 364 213 16 2 341 750 1077 1.00e-11 69.3
Msa1187720 AT5G36930.4 28.723 188 118 2 12 185 703 888 1.56e-11 68.6
Msa1187720 AT5G36930.1 28.723 188 118 2 12 185 703 888 1.56e-11 68.6
Msa1187720 AT5G36930.2 28.723 188 118 2 12 185 706 891 1.57e-11 68.6
Msa1187720 AT5G36930.3 28.723 188 118 2 12 185 703 888 1.82e-11 68.6
Msa1187720 AT5G45060.2 24.101 278 176 9 2 265 726 982 5.07e-11 67.0
Msa1187720 AT5G45060.1 24.101 278 176 9 2 265 726 982 5.07e-11 67.0
Msa1187720 AT5G45200.1 26.645 304 169 12 2 265 752 1041 7.61e-11 66.6

Find 171 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTCAGAAATTGCAACTTTA+AGG 0.128822 8_1:+27599159 Msa1187720:CDS
TCCATCTTCCCCAATATTTC+TGG 0.129400 8_1:-27596750 None:intergenic
GTGGAGATTCAATATCTTTC+TGG 0.141684 8_1:+27597856 Msa1187720:CDS
CAAAGAAAACATCTTGTTTC+CGG 0.169239 8_1:-27597793 None:intergenic
TACATGGATTCCATTATATT+TGG 0.181653 8_1:-27598174 None:intergenic
GCACAGTGGTTTGGTCTTCC+TGG 0.214345 8_1:-27598407 None:intergenic
ACTGGAGTTATTGATGATTA+AGG 0.232756 8_1:+27598815 Msa1187720:CDS
CACAGTCTCGTGCTGAATTC+TGG 0.251793 8_1:-27599045 None:intergenic
ATTTGTCTGTGCCTTGTTAT+TGG 0.253995 8_1:+27597900 Msa1187720:CDS
CGAGAGTTTCCCAGAAATAT+TGG 0.259548 8_1:+27596740 Msa1187720:five_prime_UTR
CTTCAATTCAAAACCTTAAT+CGG 0.261544 8_1:+27596823 Msa1187720:CDS
ATCTTTGAGAGAACTTATTC+TGG 0.269120 8_1:+27597274 Msa1187720:CDS
GCACGCAGTCATCTATCTTT+AGG 0.274344 8_1:-27597154 None:intergenic
CACATTTACCAATGTTTGTT+GGG 0.297193 8_1:-27598237 None:intergenic
TTCATCTCTATTGTCACCTT+GGG 0.297294 8_1:-27598918 None:intergenic
ATCCAAGAGCTGACGAAGTT+TGG 0.303358 8_1:-27598874 None:intergenic
TGCAAGCTTGTTAGTGAATC+AGG 0.310293 8_1:+27597388 Msa1187720:CDS
TCTCCCAAAGGCATCTTTAC+TGG 0.311797 8_1:-27598510 None:intergenic
TATTTGGCAACCACTTTGAT+TGG 0.323181 8_1:-27598158 None:intergenic
TTTGTCTGTGCCTTGTTATT+GGG 0.323547 8_1:+27597901 Msa1187720:CDS
TTGCCATTGAGGAACACTTT+GGG 0.333133 8_1:-27597960 None:intergenic
GGCTGAAAATAGTAGGAAAA+AGG 0.333719 8_1:+27599130 Msa1187720:CDS
GTCCTTCCATTGATCCTTGC+CGG 0.338475 8_1:-27599069 None:intergenic
CACTCTGGAATCTTTGTTCC+TGG 0.341186 8_1:-27597816 None:intergenic
GTTTGCCAATGTGAAAGATT+TGG 0.346979 8_1:+27597067 Msa1187720:CDS
CAACAGTGGCTTGGAATTCA+TGG 0.348439 8_1:-27598983 None:intergenic
AAAGTGGTTGCCAAATATAA+TGG 0.350054 8_1:+27598164 Msa1187720:CDS
GAGAGTTTCCCAGAAATATT+GGG 0.352225 8_1:+27596741 Msa1187720:five_prime_UTR
GGCAAGAAAACCATATCTTC+TGG 0.353901 8_1:+27598741 Msa1187720:CDS
CACAGTGGTTTGGTCTTCCT+GGG 0.354035 8_1:-27598406 None:intergenic
ATCAGGTAGTCTACAAGTTC+AGG 0.358501 8_1:+27598000 Msa1187720:CDS
AAGATGAAAGAAAACATTCT+TGG 0.366204 8_1:+27599428 Msa1187720:CDS
ATTTCCAGTAAAGATGCCTT+TGG 0.366306 8_1:+27598506 Msa1187720:CDS
AGAACTTACCCCAGAAGATA+TGG 0.370636 8_1:-27598751 None:intergenic
CTTCATCTCTATTGTCACCT+TGG 0.372573 8_1:-27598919 None:intergenic
TCCTTCCCATAGGTCTGTCT+TGG 0.378407 8_1:+27597045 Msa1187720:CDS
CCATCTTCCCCAATATTTCT+GGG 0.382320 8_1:-27596749 None:intergenic
TTCCAAACTTCGTCAGCTCT+TGG 0.382450 8_1:+27598872 Msa1187720:CDS
TCTTATTAAGCTGGCCGGTT+AGG 0.388508 8_1:-27599409 None:intergenic
TTAACAGAGCTTCGTATAAT+CGG 0.391776 8_1:+27596777 Msa1187720:CDS
CCCAGAAATATTGGGGAAGA+TGG 0.394468 8_1:+27596749 Msa1187720:exon
GCAGAGAAATGATCCAAGTT+TGG 0.396675 8_1:-27597335 None:intergenic
TGTAAGGTCTATGTATTTCA+AGG 0.398123 8_1:-27597240 None:intergenic
TGTGTTGAATCTCAAGAATC+AGG 0.398962 8_1:+27599220 Msa1187720:CDS
GTGAAAGATTTGGACCTATC+CGG 0.403332 8_1:+27597077 Msa1187720:CDS
AAGAATAGTGAAATTATTCC+CGG 0.403620 8_1:-27597096 None:intergenic
AGGATCTTATTACCAACAAT+GGG 0.411889 8_1:+27598132 Msa1187720:CDS
AGTAGCATAAGCTTCATTAT+CGG 0.413443 8_1:-27598562 None:intergenic
TGACAGGGTTCTGCCAAACT+TGG 0.414150 8_1:+27597322 Msa1187720:CDS
GGGCTAAACTTGACTGAGAT+AGG 0.418290 8_1:-27597939 None:intergenic
GAAGGTTTCTTTGTACATCA+AGG 0.418734 8_1:+27599109 Msa1187720:CDS
CGAACAGTCCAGTGGCAATC+AGG 0.418755 8_1:+27597983 Msa1187720:CDS
TCCCATTCTTCACTTATGTC+CGG 0.421817 8_1:-27596963 None:intergenic
TCACATTTACCAATGTTTGT+TGG 0.421838 8_1:-27598238 None:intergenic
AATAGAAAACTATGCGTTAG+TGG 0.421946 8_1:+27598720 Msa1187720:CDS
AGGTGACCCCACGATTGAAT+CGG 0.426236 8_1:-27598270 None:intergenic
AAGATTGACATTCTTCTGTA+AGG 0.427098 8_1:-27597256 None:intergenic
TTTCCAGTAAAGATGCCTTT+GGG 0.434197 8_1:+27598507 Msa1187720:CDS
TTTCGAGATGTGAAGGGTTG+TGG 0.435104 8_1:+27596931 Msa1187720:CDS
TACTACTTTGTTGAGATACA+TGG 0.435509 8_1:-27598190 None:intergenic
TCAAAGAGTGTCACTTTCTC+AGG 0.436276 8_1:+27597129 Msa1187720:CDS
TCATGTGGTGGTTTCATATG+AGG 0.442036 8_1:+27598112 Msa1187720:CDS
TTTATCTTCGAGAAATTGAC+AGG 0.444611 8_1:+27597306 Msa1187720:CDS
TTTCTTTCATCTTATTAAGC+TGG 0.454296 8_1:-27599418 None:intergenic
TTTGTTGGGGATTGCTGAAT+CGG 0.455265 8_1:-27598223 None:intergenic
ACCGGACATAAGTGAAGAAT+GGG 0.456105 8_1:+27596962 Msa1187720:CDS
GTTGCCATTGAGGAACACTT+TGG 0.458585 8_1:-27597961 None:intergenic
GCAAGAAAACCATATCTTCT+GGG 0.462088 8_1:+27598742 Msa1187720:CDS
TAGAGAGACTATTAATTCAC+TGG 0.463466 8_1:+27598797 Msa1187720:CDS
TTTAAGAGAGGTCAATATGA+TGG 0.466004 8_1:-27596688 None:intergenic
ATCATCCGAGTCAGAACCTT+CGG 0.466988 8_1:-27598682 None:intergenic
ACATCAAGGCTGAAAATAGT+AGG 0.467304 8_1:+27599123 Msa1187720:CDS
CTGTGGCCGGCAAGGATCAA+TGG 0.470479 8_1:+27599063 Msa1187720:CDS
TTTGATTGGAACCCCATTGT+TGG 0.471015 8_1:-27598144 None:intergenic
ATATCTTCTGGGGTAAGTTC+TGG 0.471326 8_1:+27598753 Msa1187720:CDS
AACCGGACATAAGTGAAGAA+TGG 0.473396 8_1:+27596961 Msa1187720:CDS
GGTGACCCCACGATTGAATC+GGG 0.474584 8_1:-27598269 None:intergenic
ACAAGGCACAGACAAATTGC+AGG 0.478724 8_1:-27597894 None:intergenic
TGAAAGATTTGGACCTATCC+GGG 0.481393 8_1:+27597078 Msa1187720:CDS
AGAGTAGGCTCAAGTGGACT+TGG 0.482501 8_1:+27599378 Msa1187720:CDS
AGCCCCAAAGTGTTCCTCAA+TGG 0.485552 8_1:+27597957 Msa1187720:CDS
AGGGAGACAGCACAGTGGTT+TGG 0.488924 8_1:-27598416 None:intergenic
ATGCTAAAATTTGAAGATAA+CGG 0.488952 8_1:+27598059 Msa1187720:CDS
AGGGTTGTGGTTATCTAAAC+CGG 0.489591 8_1:+27596944 Msa1187720:CDS
TTCAATCCCGATTCAATCGT+GGG 0.492151 8_1:+27598263 Msa1187720:CDS
ACTTGGTCTATTGACCTAAC+CGG 0.494695 8_1:+27599395 Msa1187720:CDS
TAGAGCAGAGTAGGCTCAAG+TGG 0.496961 8_1:+27599372 Msa1187720:CDS
CAGCTGAAATAGAGCAGAGT+AGG 0.498400 8_1:+27599363 Msa1187720:CDS
ACAGCTAGCTTCCAAAGAAA+AGG 0.499290 8_1:+27599184 Msa1187720:CDS
CCACCCTCTGAAATCAAAAG+AGG 0.503404 8_1:+27598465 Msa1187720:CDS
TTGATTTCAGAGGGTGGAGA+AGG 0.503515 8_1:-27598459 None:intergenic
GAAGAATTATGAGAATCATC+TGG 0.505535 8_1:+27599022 Msa1187720:CDS
AAATTCTGTCTTCGAAGACT+TGG 0.512096 8_1:-27596988 None:intergenic
GATTCAACACAAGTTGTTCG+AGG 0.516813 8_1:-27599208 None:intergenic
CGAGTCAGAACCTTCGGTGT+TGG 0.518580 8_1:-27598676 None:intergenic
TCACAGATTCAACAGTGGCT+TGG 0.518584 8_1:-27598992 None:intergenic
GGCATCTTTACTGGAAATGT+AGG 0.519141 8_1:-27598501 None:intergenic
GAAAACATGGCAATGCTACT+TGG 0.520021 8_1:-27596888 None:intergenic
TCTGTGCCTTGTTATTGGGC+AGG 0.525049 8_1:+27597905 Msa1187720:CDS
GAAAGTGTTTCTAATTTGCA+TGG 0.526053 8_1:-27597200 None:intergenic
AAAGATGCCTTTGGGAGATG+TGG 0.528159 8_1:+27598515 Msa1187720:CDS
CAAGGTGACAATAGAGATGA+AGG 0.529920 8_1:+27598920 Msa1187720:CDS
GGCAAACCAAGACAGACCTA+TGG 0.529986 8_1:-27597051 None:intergenic
GGAAGAAGACTCTTCTTCAC+AGG 0.533956 8_1:-27598655 None:intergenic
TTTCTATTAACTAAGTCGAA+GGG 0.537656 8_1:-27598705 None:intergenic
CAGCAATCCCCAACAAACAT+TGG 0.541571 8_1:+27598229 Msa1187720:CDS
ATGTGTGTTTCGAGATGTGA+AGG 0.542705 8_1:+27596924 Msa1187720:CDS
TCTCTATTGTCACCTTGGGG+TGG 0.549648 8_1:-27598914 None:intergenic
GAGGATCTTATTACCAACAA+TGG 0.554704 8_1:+27598131 Msa1187720:CDS
TAGTGAAATTATTCCCGGAT+AGG 0.556157 8_1:-27597091 None:intergenic
CGACAGGAAATGATTGAAGC+CGG 0.557548 8_1:+27597774 Msa1187720:intron
TGCCATTGAGGAACACTTTG+GGG 0.561919 8_1:-27597959 None:intergenic
TATTGCTGAGTTCAAGAGAA+AGG 0.566425 8_1:+27598947 Msa1187720:CDS
TCAGATGATTTCCTTCCCAT+AGG 0.566951 8_1:+27597035 Msa1187720:CDS
ACATTTACCAATGTTTGTTG+GGG 0.567699 8_1:-27598236 None:intergenic
CAAACCAAGTTGTGAAAACA+TGG 0.570025 8_1:-27596901 None:intergenic
TTTCATCTTATTAAGCTGGC+CGG 0.571028 8_1:-27599414 None:intergenic
CATCCGCCTGCCCAATAACA+AGG 0.573139 8_1:-27597911 None:intergenic
TGGACTGTTCGTTGCCATTG+AGG 0.573399 8_1:-27597971 None:intergenic
ATATTGAATCTCCACTTGTA+CGG 0.575170 8_1:-27597848 None:intergenic
TTTCAATCCCGATTCAATCG+TGG 0.575753 8_1:+27598262 Msa1187720:CDS
GTAGACTACCTGATTGCCAC+TGG 0.579096 8_1:-27597991 None:intergenic
ATTCAGCACGAGACTGTGGC+CGG 0.579952 8_1:+27599050 Msa1187720:CDS
AGAGAGGTCAATATGATGGG+TGG 0.580171 8_1:-27596684 None:intergenic
GGGGTCACCTCAAAGAGTAA+AGG 0.582126 8_1:+27598283 Msa1187720:CDS
AAGATGCCTTTGGGAGATGT+GGG 0.586108 8_1:+27598516 Msa1187720:CDS
AGAACTACAAATCTGTGGAA+TGG 0.586446 8_1:+27596857 Msa1187720:CDS
TGAGGGTGATGATGTCGAGC+TGG 0.589014 8_1:+27598623 Msa1187720:CDS
CAACACCGAAGGTTCTGACT+CGG 0.591531 8_1:+27598677 Msa1187720:CDS
CTCATTCAAAGATGTAGTGA+GGG 0.592554 8_1:+27598606 Msa1187720:CDS
GAATTCATGGAATGATGCAA+AGG 0.596317 8_1:-27598970 None:intergenic
CGAAGTTTGGAATGTGCGGA+AGG 0.596748 8_1:-27598861 None:intergenic
TTAAGAGAGGTCAATATGAT+GGG 0.598217 8_1:-27596687 None:intergenic
AGATTGTGTTGATGTGAGTG+AGG 0.603379 8_1:-27598436 None:intergenic
TCACTACATCTTTGAATGAG+AGG 0.604939 8_1:-27598603 None:intergenic
ACAGTGGTTTGGTCTTCCTG+GGG 0.606601 8_1:-27598405 None:intergenic
GCAAACCAAGACAGACCTAT+GGG 0.611839 8_1:-27597050 None:intergenic
TCATCTCTATTGTCACCTTG+GGG 0.613937 8_1:-27598917 None:intergenic
CAGAATTCAGCACGAGACTG+TGG 0.614851 8_1:+27599046 Msa1187720:CDS
TCTCATTCAAAGATGTAGTG+AGG 0.615666 8_1:+27598605 Msa1187720:CDS
ATCATCTGATAAATTGCAGT+AGG 0.618009 8_1:-27597021 None:intergenic
GAGTCTTCTTCCAACACCGA+AGG 0.618932 8_1:+27598666 Msa1187720:CDS
GTACGGTGTGAAAACCACTC+TGG 0.623125 8_1:-27597831 None:intergenic
TAGGTCCAAATCTTTCACAT+TGG 0.626169 8_1:-27597072 None:intergenic
AAATTAGAACTACAAATCTG+TGG 0.626177 8_1:+27596852 Msa1187720:CDS
TCTCTTCCCACATCTCCCAA+AGG 0.627588 8_1:-27598522 None:intergenic
CTGACGAAGTTTGGAATGTG+CGG 0.628337 8_1:-27598865 None:intergenic
AAAGTTGAGCTGTTTAAGAG+AGG 0.629374 8_1:-27596700 None:intergenic
GGCCGGCAAGGATCAATGGA+AGG 0.631326 8_1:+27599067 Msa1187720:CDS
GTGCCTTGTTATTGGGCAGG+CGG 0.633594 8_1:+27597908 Msa1187720:CDS
GATTATGACTAAGAGACCCC+AGG 0.634497 8_1:+27598389 Msa1187720:intron
TGTGTGTTTCGAGATGTGAA+GGG 0.635378 8_1:+27596925 Msa1187720:CDS
ACCAAGACAGACCTATGGGA+AGG 0.641967 8_1:-27597046 None:intergenic
GAGTGCAAGTAGCCACCCCA+AGG 0.646264 8_1:+27598902 Msa1187720:CDS
AGCGGTGAGATAAAACATGA+TGG 0.648523 8_1:+27599335 Msa1187720:CDS
CAAGAAAACCATATCTTCTG+GGG 0.650883 8_1:+27598743 Msa1187720:CDS
CAATGGGGTTCCAATCAAAG+TGG 0.653320 8_1:+27598148 Msa1187720:CDS
TCAATCCCGATTCAATCGTG+GGG 0.660470 8_1:+27598264 Msa1187720:CDS
CAAGGCACAGACAAATTGCA+GGG 0.661851 8_1:-27597893 None:intergenic
GATTGTGTTGATGTGAGTGA+GGG 0.663041 8_1:-27598435 None:intergenic
AGAGTTTCCCAGAAATATTG+GGG 0.664942 8_1:+27596742 Msa1187720:five_prime_UTR
GATGATCTACTTGCTTCAAG+CGG 0.694403 8_1:+27599317 Msa1187720:CDS
CAGGAACAAAGATTCCAGAG+TGG 0.696773 8_1:+27597817 Msa1187720:CDS
AATGGCAACGAACAGTCCAG+TGG 0.699808 8_1:+27597975 Msa1187720:CDS
TTATCTTCGAGAAATTGACA+GGG 0.715676 8_1:+27597307 Msa1187720:CDS
CAATGAGTGGAATCATGTGG+TGG 0.723623 8_1:+27598100 Msa1187720:CDS
GGTGACAATAGAGATGAAGG+AGG 0.725003 8_1:+27598923 Msa1187720:CDS
CTTACTCACAGATTCAACAG+TGG 0.730006 8_1:-27598997 None:intergenic
GGATCTTATTACCAACAATG+GGG 0.731369 8_1:+27598133 Msa1187720:CDS
AGACAATGAGTGGAATCATG+TGG 0.742228 8_1:+27598097 Msa1187720:CDS
GAGTGAGGGAGACAGCACAG+TGG 0.813091 8_1:-27598421 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTCATGTTGATTATTA+TGG + chr8_1:27597466-27597485 Msa1187720:intron 15.0%
!! AAATGTTAGAAACTTATAAA+AGG - chr8_1:27597623-27597642 None:intergenic 15.0%
!! AACTTATAAAAGGTTACTAT+GGG - chr8_1:27597613-27597632 None:intergenic 20.0%
!! AAACTTATAAAAGGTTACTA+TGG - chr8_1:27597614-27597633 None:intergenic 20.0%
!! TAATATCAGTTTCTATCAAA+TGG + chr8_1:27597687-27597706 Msa1187720:intron 20.0%
!! AATATCAGTTTCTATCAAAT+GGG + chr8_1:27597688-27597707 Msa1187720:intron 20.0%
!!! ATCATATACTTTTGTTTGAT+GGG + chr8_1:27598033-27598052 Msa1187720:CDS 20.0%
!! ATGCTAAAATTTGAAGATAA+CGG + chr8_1:27598059-27598078 Msa1187720:CDS 20.0%
!! CTTCAAAAAATCAATAAAGA+AGG + chr8_1:27599091-27599110 Msa1187720:CDS 20.0%
! CTTCAATTCAAAACCTTAAT+CGG + chr8_1:27596823-27596842 Msa1187720:CDS 25.0%
! AAATTAGAACTACAAATCTG+TGG + chr8_1:27596852-27596871 Msa1187720:CDS 25.0%
! AAGAATAGTGAAATTATTCC+CGG - chr8_1:27597099-27597118 None:intergenic 25.0%
! AATAAAACAGAGATCAAAAG+AGG - chr8_1:27597416-27597435 None:intergenic 25.0%
! ACTTATAAAAGGTTACTATG+GGG - chr8_1:27597612-27597631 None:intergenic 25.0%
!! TAGAAGTTTGAGATATTGAT+AGG - chr8_1:27597722-27597741 None:intergenic 25.0%
!!! GATCATATACTTTTGTTTGA+TGG + chr8_1:27598032-27598051 Msa1187720:CDS 25.0%
! TACATGGATTCCATTATATT+TGG - chr8_1:27598177-27598196 None:intergenic 25.0%
!! AATTTTACCTTTACTCTTTG+AGG - chr8_1:27598293-27598312 None:intergenic 25.0%
! TTTCTATTAACTAAGTCGAA+GGG - chr8_1:27598708-27598727 None:intergenic 25.0%
!! TTTTCTATTAACTAAGTCGA+AGG - chr8_1:27598709-27598728 None:intergenic 25.0%
! TTTCTTTCATCTTATTAAGC+TGG - chr8_1:27599421-27599440 None:intergenic 25.0%
! AAGATGAAAGAAAACATTCT+TGG + chr8_1:27599428-27599447 Msa1187720:CDS 25.0%
TTAAGAGAGGTCAATATGAT+GGG - chr8_1:27596690-27596709 None:intergenic 30.0%
TTTAAGAGAGGTCAATATGA+TGG - chr8_1:27596691-27596710 None:intergenic 30.0%
TTAACAGAGCTTCGTATAAT+CGG + chr8_1:27596777-27596796 Msa1187720:CDS 30.0%
!!! TTAAGGTTTTGAATTGAAGG+TGG - chr8_1:27596822-27596841 None:intergenic 30.0%
!!! CGATTAAGGTTTTGAATTGA+AGG - chr8_1:27596825-27596844 None:intergenic 30.0%
ATCATCTGATAAATTGCAGT+AGG - chr8_1:27597024-27597043 None:intergenic 30.0%
!! GAAAGTGTTTCTAATTTGCA+TGG - chr8_1:27597203-27597222 None:intergenic 30.0%
TGTAAGGTCTATGTATTTCA+AGG - chr8_1:27597243-27597262 None:intergenic 30.0%
AAGATTGACATTCTTCTGTA+AGG - chr8_1:27597259-27597278 None:intergenic 30.0%
! ATCTTTGAGAGAACTTATTC+TGG + chr8_1:27597274-27597293 Msa1187720:CDS 30.0%
TTTATCTTCGAGAAATTGAC+AGG + chr8_1:27597306-27597325 Msa1187720:CDS 30.0%
TTATCTTCGAGAAATTGACA+GGG + chr8_1:27597307-27597326 Msa1187720:CDS 30.0%
! AGTCAATAAAGTGCAGTTTT+TGG - chr8_1:27597360-27597379 None:intergenic 30.0%
! AGTTGATGCTATAATGTATC+AGG + chr8_1:27597505-27597524 Msa1187720:intron 30.0%
! CAAAGAAAACATCTTGTTTC+CGG - chr8_1:27597796-27597815 None:intergenic 30.0%
ATATTGAATCTCCACTTGTA+CGG - chr8_1:27597851-27597870 None:intergenic 30.0%
AGGATCTTATTACCAACAAT+GGG + chr8_1:27598132-27598151 Msa1187720:CDS 30.0%
AAAGTGGTTGCCAAATATAA+TGG + chr8_1:27598164-27598183 Msa1187720:CDS 30.0%
TACTACTTTGTTGAGATACA+TGG - chr8_1:27598193-27598212 None:intergenic 30.0%
! ACATTTACCAATGTTTGTTG+GGG - chr8_1:27598239-27598258 None:intergenic 30.0%
CACATTTACCAATGTTTGTT+GGG - chr8_1:27598240-27598259 None:intergenic 30.0%
TCACATTTACCAATGTTTGT+TGG - chr8_1:27598241-27598260 None:intergenic 30.0%
GATGTATAACAGGATTAACA+GGG - chr8_1:27598331-27598350 None:intergenic 30.0%
TGATGTATAACAGGATTAAC+AGG - chr8_1:27598332-27598351 None:intergenic 30.0%
AGTAGCATAAGCTTCATTAT+CGG - chr8_1:27598565-27598584 None:intergenic 30.0%
AATAGAAAACTATGCGTTAG+TGG + chr8_1:27598720-27598739 Msa1187720:CDS 30.0%
TAGAGAGACTATTAATTCAC+TGG + chr8_1:27598797-27598816 Msa1187720:CDS 30.0%
! ACTGGAGTTATTGATGATTA+AGG + chr8_1:27598815-27598834 Msa1187720:CDS 30.0%
GAAGAATTATGAGAATCATC+TGG + chr8_1:27599022-27599041 Msa1187720:CDS 30.0%
AGTCAGAAATTGCAACTTTA+AGG + chr8_1:27599159-27599178 Msa1187720:CDS 30.0%
AAAGTTGAGCTGTTTAAGAG+AGG - chr8_1:27596703-27596722 None:intergenic 35.0%
GAGAGTTTCCCAGAAATATT+GGG + chr8_1:27596741-27596760 Msa1187720:five_prime_UTR 35.0%
AGAGTTTCCCAGAAATATTG+GGG + chr8_1:27596742-27596761 Msa1187720:five_prime_UTR 35.0%
! GAAGGTGGCAATTCTTTTAT+AGG - chr8_1:27596807-27596826 None:intergenic 35.0%
! CTAATTTTCGAAGCCGATTA+AGG - chr8_1:27596839-27596858 None:intergenic 35.0%
AGAACTACAAATCTGTGGAA+TGG + chr8_1:27596857-27596876 Msa1187720:CDS 35.0%
! ATTGCCATGTTTTCACAACT+TGG + chr8_1:27596897-27596916 Msa1187720:CDS 35.0%
CAAACCAAGTTGTGAAAACA+TGG - chr8_1:27596904-27596923 None:intergenic 35.0%
AAATTCTGTCTTCGAAGACT+TGG - chr8_1:27596991-27597010 None:intergenic 35.0%
GTTTGCCAATGTGAAAGATT+TGG + chr8_1:27597067-27597086 Msa1187720:CDS 35.0%
TAGGTCCAAATCTTTCACAT+TGG - chr8_1:27597075-27597094 None:intergenic 35.0%
TAGTGAAATTATTCCCGGAT+AGG - chr8_1:27597094-27597113 None:intergenic 35.0%
CTATAATGTATCAGGCAGAT+TGG + chr8_1:27597513-27597532 Msa1187720:intron 35.0%
TTTCTAAACTTATCACCGAC+AGG + chr8_1:27597758-27597777 Msa1187720:intron 35.0%
! AACAAGATGTTTTCTTTGCC+AGG + chr8_1:27597798-27597817 Msa1187720:CDS 35.0%
GTGGAGATTCAATATCTTTC+TGG + chr8_1:27597856-27597875 Msa1187720:CDS 35.0%
! ATTTGTCTGTGCCTTGTTAT+TGG + chr8_1:27597900-27597919 Msa1187720:CDS 35.0%
!! TTTGTCTGTGCCTTGTTATT+GGG + chr8_1:27597901-27597920 Msa1187720:CDS 35.0%
! TAGTGTTTTCAGACAATGAG+TGG + chr8_1:27598087-27598106 Msa1187720:CDS 35.0%
GAGGATCTTATTACCAACAA+TGG + chr8_1:27598131-27598150 Msa1187720:CDS 35.0%
GGATCTTATTACCAACAATG+GGG + chr8_1:27598133-27598152 Msa1187720:CDS 35.0%
TATTTGGCAACCACTTTGAT+TGG - chr8_1:27598161-27598180 None:intergenic 35.0%
!!! ATGCCTCTTTTGATTTCAGA+GGG - chr8_1:27598471-27598490 None:intergenic 35.0%
!!! AATGCCTCTTTTGATTTCAG+AGG - chr8_1:27598472-27598491 None:intergenic 35.0%
ATTTCCAGTAAAGATGCCTT+TGG + chr8_1:27598506-27598525 Msa1187720:CDS 35.0%
TTTCCAGTAAAGATGCCTTT+GGG + chr8_1:27598507-27598526 Msa1187720:CDS 35.0%
TCACTACATCTTTGAATGAG+AGG - chr8_1:27598606-27598625 None:intergenic 35.0%
TCTCATTCAAAGATGTAGTG+AGG + chr8_1:27598605-27598624 Msa1187720:CDS 35.0%
CTCATTCAAAGATGTAGTGA+GGG + chr8_1:27598606-27598625 Msa1187720:CDS 35.0%
GCAAGAAAACCATATCTTCT+GGG + chr8_1:27598742-27598761 Msa1187720:CDS 35.0%
CAAGAAAACCATATCTTCTG+GGG + chr8_1:27598743-27598762 Msa1187720:CDS 35.0%
TTCATCTCTATTGTCACCTT+GGG - chr8_1:27598921-27598940 None:intergenic 35.0%
TATTGCTGAGTTCAAGAGAA+AGG + chr8_1:27598947-27598966 Msa1187720:CDS 35.0%
GAATTCATGGAATGATGCAA+AGG - chr8_1:27598973-27598992 None:intergenic 35.0%
GAAGGTTTCTTTGTACATCA+AGG + chr8_1:27599109-27599128 Msa1187720:CDS 35.0%
ACATCAAGGCTGAAAATAGT+AGG + chr8_1:27599123-27599142 Msa1187720:CDS 35.0%
GGCTGAAAATAGTAGGAAAA+AGG + chr8_1:27599130-27599149 Msa1187720:CDS 35.0%
TGTGTTGAATCTCAAGAATC+AGG + chr8_1:27599220-27599239 Msa1187720:CDS 35.0%
TTTCATCTTATTAAGCTGGC+CGG - chr8_1:27599417-27599436 None:intergenic 35.0%
CGAGAGTTTCCCAGAAATAT+TGG + chr8_1:27596740-27596759 Msa1187720:five_prime_UTR 40.0%
CCATCTTCCCCAATATTTCT+GGG - chr8_1:27596752-27596771 None:intergenic 40.0%
TCCATCTTCCCCAATATTTC+TGG - chr8_1:27596753-27596772 None:intergenic 40.0%
GAAAACATGGCAATGCTACT+TGG - chr8_1:27596891-27596910 None:intergenic 40.0%
ATGTGTGTTTCGAGATGTGA+AGG + chr8_1:27596924-27596943 Msa1187720:CDS 40.0%
TGTGTGTTTCGAGATGTGAA+GGG + chr8_1:27596925-27596944 Msa1187720:CDS 40.0%
AGGGTTGTGGTTATCTAAAC+CGG + chr8_1:27596944-27596963 Msa1187720:CDS 40.0%
AACCGGACATAAGTGAAGAA+TGG + chr8_1:27596961-27596980 Msa1187720:CDS 40.0%
ACCGGACATAAGTGAAGAAT+GGG + chr8_1:27596962-27596981 Msa1187720:CDS 40.0%
TCCCATTCTTCACTTATGTC+CGG - chr8_1:27596966-27596985 None:intergenic 40.0%
TCAGATGATTTCCTTCCCAT+AGG + chr8_1:27597035-27597054 Msa1187720:CDS 40.0%
GTGAAAGATTTGGACCTATC+CGG + chr8_1:27597077-27597096 Msa1187720:CDS 40.0%
TGAAAGATTTGGACCTATCC+GGG + chr8_1:27597078-27597097 Msa1187720:CDS 40.0%
!! TCAAAGAGTGTCACTTTCTC+AGG + chr8_1:27597129-27597148 Msa1187720:CDS 40.0%
GCAGAGAAATGATCCAAGTT+TGG - chr8_1:27597338-27597357 None:intergenic 40.0%
!! TGCAAGCTTGTTAGTGAATC+AGG + chr8_1:27597388-27597407 Msa1187720:CDS 40.0%
!!! GATTGGTTGATGGAGTGTTT+TGG + chr8_1:27597530-27597549 Msa1187720:intron 40.0%
TTGCCATTGAGGAACACTTT+GGG - chr8_1:27597963-27597982 None:intergenic 40.0%
ATCAGGTAGTCTACAAGTTC+AGG + chr8_1:27598000-27598019 Msa1187720:CDS 40.0%
AGACAATGAGTGGAATCATG+TGG + chr8_1:27598097-27598116 Msa1187720:CDS 40.0%
TCATGTGGTGGTTTCATATG+AGG + chr8_1:27598112-27598131 Msa1187720:CDS 40.0%
TTTGATTGGAACCCCATTGT+TGG - chr8_1:27598147-27598166 None:intergenic 40.0%
TTTGTTGGGGATTGCTGAAT+CGG - chr8_1:27598226-27598245 None:intergenic 40.0%
TTTCAATCCCGATTCAATCG+TGG + chr8_1:27598262-27598281 Msa1187720:CDS 40.0%
TTCAATCCCGATTCAATCGT+GGG + chr8_1:27598263-27598282 Msa1187720:CDS 40.0%
GAGCATGAGTGATGTATAAC+AGG - chr8_1:27598341-27598360 None:intergenic 40.0%
! GATTGTGTTGATGTGAGTGA+GGG - chr8_1:27598438-27598457 None:intergenic 40.0%
! AGATTGTGTTGATGTGAGTG+AGG - chr8_1:27598439-27598458 None:intergenic 40.0%
GGCATCTTTACTGGAAATGT+AGG - chr8_1:27598504-27598523 None:intergenic 40.0%
GGCAAGAAAACCATATCTTC+TGG + chr8_1:27598741-27598760 Msa1187720:CDS 40.0%
AGAACTTACCCCAGAAGATA+TGG - chr8_1:27598754-27598773 None:intergenic 40.0%
!! ATATCTTCTGGGGTAAGTTC+TGG + chr8_1:27598753-27598772 Msa1187720:CDS 40.0%
TCATCTCTATTGTCACCTTG+GGG - chr8_1:27598920-27598939 None:intergenic 40.0%
CTTCATCTCTATTGTCACCT+TGG - chr8_1:27598922-27598941 None:intergenic 40.0%
CAAGGTGACAATAGAGATGA+AGG + chr8_1:27598920-27598939 Msa1187720:CDS 40.0%
CTTACTCACAGATTCAACAG+TGG - chr8_1:27599000-27599019 None:intergenic 40.0%
! ACAGCTAGCTTCCAAAGAAA+AGG + chr8_1:27599184-27599203 Msa1187720:CDS 40.0%
! TTGTTCGAGGTCCTTTTCTT+TGG - chr8_1:27599198-27599217 None:intergenic 40.0%
GATTCAACACAAGTTGTTCG+AGG - chr8_1:27599211-27599230 None:intergenic 40.0%
GATGATCTACTTGCTTCAAG+CGG + chr8_1:27599317-27599336 Msa1187720:CDS 40.0%
AGCGGTGAGATAAAACATGA+TGG + chr8_1:27599335-27599354 Msa1187720:CDS 40.0%
ACTTGGTCTATTGACCTAAC+CGG + chr8_1:27599395-27599414 Msa1187720:CDS 40.0%
! AGAGAGGTCAATATGATGGG+TGG - chr8_1:27596687-27596706 None:intergenic 45.0%
CCCAGAAATATTGGGGAAGA+TGG + chr8_1:27596749-27596768 Msa1187720:exon 45.0%
TTTCGAGATGTGAAGGGTTG+TGG + chr8_1:27596931-27596950 Msa1187720:CDS 45.0%
GCAAACCAAGACAGACCTAT+GGG - chr8_1:27597053-27597072 None:intergenic 45.0%
GCACGCAGTCATCTATCTTT+AGG - chr8_1:27597157-27597176 None:intergenic 45.0%
! GTATCAGGCAGATTGGTTGA+TGG + chr8_1:27597520-27597539 Msa1187720:intron 45.0%
CGGCTTCAATCATTTCCTGT+CGG - chr8_1:27597776-27597795 None:intergenic 45.0%
CGACAGGAAATGATTGAAGC+CGG + chr8_1:27597774-27597793 Msa1187720:intron 45.0%
!! CACTCTGGAATCTTTGTTCC+TGG - chr8_1:27597819-27597838 None:intergenic 45.0%
CAGGAACAAAGATTCCAGAG+TGG + chr8_1:27597817-27597836 Msa1187720:CDS 45.0%
! TGGTTTTCACACCGTACAAG+TGG + chr8_1:27597837-27597856 Msa1187720:CDS 45.0%
CAAGGCACAGACAAATTGCA+GGG - chr8_1:27597896-27597915 None:intergenic 45.0%
ACAAGGCACAGACAAATTGC+AGG - chr8_1:27597897-27597916 None:intergenic 45.0%
GGGCTAAACTTGACTGAGAT+AGG - chr8_1:27597942-27597961 None:intergenic 45.0%
TGCCATTGAGGAACACTTTG+GGG - chr8_1:27597962-27597981 None:intergenic 45.0%
GTTGCCATTGAGGAACACTT+TGG - chr8_1:27597964-27597983 None:intergenic 45.0%
CAATGAGTGGAATCATGTGG+TGG + chr8_1:27598100-27598119 Msa1187720:CDS 45.0%
CAATGGGGTTCCAATCAAAG+TGG + chr8_1:27598148-27598167 Msa1187720:CDS 45.0%
CAGCAATCCCCAACAAACAT+TGG + chr8_1:27598229-27598248 Msa1187720:CDS 45.0%
TCAATCCCGATTCAATCGTG+GGG + chr8_1:27598264-27598283 Msa1187720:CDS 45.0%
GATTATGACTAAGAGACCCC+AGG + chr8_1:27598389-27598408 Msa1187720:intron 45.0%
TTGATTTCAGAGGGTGGAGA+AGG - chr8_1:27598462-27598481 None:intergenic 45.0%
!!! CCTCTTTTGATTTCAGAGGG+TGG - chr8_1:27598468-27598487 None:intergenic 45.0%
!! CCACCCTCTGAAATCAAAAG+AGG + chr8_1:27598465-27598484 Msa1187720:CDS 45.0%
!! TCTCCCAAAGGCATCTTTAC+TGG - chr8_1:27598513-27598532 None:intergenic 45.0%
! AAAGATGCCTTTGGGAGATG+TGG + chr8_1:27598515-27598534 Msa1187720:CDS 45.0%
! AAGATGCCTTTGGGAGATGT+GGG + chr8_1:27598516-27598535 Msa1187720:CDS 45.0%
GGAAGAAGACTCTTCTTCAC+AGG - chr8_1:27598658-27598677 None:intergenic 45.0%
ATCATCCGAGTCAGAACCTT+CGG - chr8_1:27598685-27598704 None:intergenic 45.0%
! CTGACGAAGTTTGGAATGTG+CGG - chr8_1:27598868-27598887 None:intergenic 45.0%
TTCCAAACTTCGTCAGCTCT+TGG + chr8_1:27598872-27598891 Msa1187720:CDS 45.0%
ATCCAAGAGCTGACGAAGTT+TGG - chr8_1:27598877-27598896 None:intergenic 45.0%
GGTGACAATAGAGATGAAGG+AGG + chr8_1:27598923-27598942 Msa1187720:CDS 45.0%
! CAACAGTGGCTTGGAATTCA+TGG - chr8_1:27598986-27599005 None:intergenic 45.0%
TCACAGATTCAACAGTGGCT+TGG - chr8_1:27598995-27599014 None:intergenic 45.0%
CAGCTGAAATAGAGCAGAGT+AGG + chr8_1:27599363-27599382 Msa1187720:CDS 45.0%
TCTTATTAAGCTGGCCGGTT+AGG - chr8_1:27599412-27599431 None:intergenic 45.0%
TCCTTCCCATAGGTCTGTCT+TGG + chr8_1:27597045-27597064 Msa1187720:CDS 50.0%
ACCAAGACAGACCTATGGGA+AGG - chr8_1:27597049-27597068 None:intergenic 50.0%
GGCAAACCAAGACAGACCTA+TGG - chr8_1:27597054-27597073 None:intergenic 50.0%
TGACAGGGTTCTGCCAAACT+TGG + chr8_1:27597322-27597341 Msa1187720:CDS 50.0%
GTACGGTGTGAAAACCACTC+TGG - chr8_1:27597834-27597853 None:intergenic 50.0%
!! TCTGTGCCTTGTTATTGGGC+AGG + chr8_1:27597905-27597924 Msa1187720:CDS 50.0%
! AGCCCCAAAGTGTTCCTCAA+TGG + chr8_1:27597957-27597976 Msa1187720:CDS 50.0%
TGGACTGTTCGTTGCCATTG+AGG - chr8_1:27597974-27597993 None:intergenic 50.0%
AATGGCAACGAACAGTCCAG+TGG + chr8_1:27597975-27597994 Msa1187720:CDS 50.0%
GTAGACTACCTGATTGCCAC+TGG - chr8_1:27597994-27598013 None:intergenic 50.0%
AGGTGACCCCACGATTGAAT+CGG - chr8_1:27598273-27598292 None:intergenic 50.0%
GGGGTCACCTCAAAGAGTAA+AGG + chr8_1:27598283-27598302 Msa1187720:CDS 50.0%
!! ACAGTGGTTTGGTCTTCCTG+GGG - chr8_1:27598408-27598427 None:intergenic 50.0%
!! CACAGTGGTTTGGTCTTCCT+GGG - chr8_1:27598409-27598428 None:intergenic 50.0%
TCTCTTCCCACATCTCCCAA+AGG - chr8_1:27598525-27598544 None:intergenic 50.0%
GAGTCTTCTTCCAACACCGA+AGG + chr8_1:27598666-27598685 Msa1187720:CDS 50.0%
! CAACACCGAAGGTTCTGACT+CGG + chr8_1:27598677-27598696 Msa1187720:CDS 50.0%
! CGAAGTTTGGAATGTGCGGA+AGG - chr8_1:27598864-27598883 None:intergenic 50.0%
TCTCTATTGTCACCTTGGGG+TGG - chr8_1:27598917-27598936 None:intergenic 50.0%
CACAGTCTCGTGCTGAATTC+TGG - chr8_1:27599048-27599067 None:intergenic 50.0%
CAGAATTCAGCACGAGACTG+TGG + chr8_1:27599046-27599065 Msa1187720:CDS 50.0%
GTCCTTCCATTGATCCTTGC+CGG - chr8_1:27599072-27599091 None:intergenic 50.0%
TAGAGCAGAGTAGGCTCAAG+TGG + chr8_1:27599372-27599391 Msa1187720:CDS 50.0%
! AGAGTAGGCTCAAGTGGACT+TGG + chr8_1:27599378-27599397 Msa1187720:CDS 50.0%
!! GTGCCTTGTTATTGGGCAGG+CGG + chr8_1:27597908-27597927 Msa1187720:CDS 55.0%
CATCCGCCTGCCCAATAACA+AGG - chr8_1:27597914-27597933 None:intergenic 55.0%
CGAACAGTCCAGTGGCAATC+AGG + chr8_1:27597983-27598002 Msa1187720:CDS 55.0%
GGTGACCCCACGATTGAATC+GGG - chr8_1:27598272-27598291 None:intergenic 55.0%
!! GCACAGTGGTTTGGTCTTCC+TGG - chr8_1:27598410-27598429 None:intergenic 55.0%
AGGGAGACAGCACAGTGGTT+TGG - chr8_1:27598419-27598438 None:intergenic 55.0%
! TGAGGGTGATGATGTCGAGC+TGG + chr8_1:27598623-27598642 Msa1187720:CDS 55.0%
! CGAGTCAGAACCTTCGGTGT+TGG - chr8_1:27598679-27598698 None:intergenic 55.0%
ATTCAGCACGAGACTGTGGC+CGG + chr8_1:27599050-27599069 Msa1187720:CDS 55.0%
GAGTGAGGGAGACAGCACAG+TGG - chr8_1:27598424-27598443 None:intergenic 60.0%
GAGTGCAAGTAGCCACCCCA+AGG + chr8_1:27598902-27598921 Msa1187720:CDS 60.0%
CTGTGGCCGGCAAGGATCAA+TGG + chr8_1:27599063-27599082 Msa1187720:CDS 60.0%
GGCCGGCAAGGATCAATGGA+AGG + chr8_1:27599067-27599086 Msa1187720:CDS 60.0%
GCACGAGACTGTGGCCGGCA+AGG + chr8_1:27599055-27599074 Msa1187720:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 27596671 27599463 27596671 ID=Msa1187720;Name=Msa1187720
chr8_1 mRNA 27596671 27599463 27596671 ID=Msa1187720-mRNA-1;Parent=Msa1187720;Name=Msa1187720-mRNA-1;_AED=0.38;_eAED=0.38;_QI=97|1|0.66|1|1|0.66|3|0|746
chr8_1 exon 27596671 27597409 27596671 ID=Msa1187720-mRNA-1:exon:9700;Parent=Msa1187720-mRNA-1
chr8_1 exon 27597780 27598304 27597780 ID=Msa1187720-mRNA-1:exon:9701;Parent=Msa1187720-mRNA-1
chr8_1 exon 27598390 27599463 27598390 ID=Msa1187720-mRNA-1:exon:9702;Parent=Msa1187720-mRNA-1
chr8_1 five_prime_UTR 27596671 27596767 27596671 ID=Msa1187720-mRNA-1:five_prime_utr;Parent=Msa1187720-mRNA-1
chr8_1 CDS 27596768 27597409 27596768 ID=Msa1187720-mRNA-1:cds;Parent=Msa1187720-mRNA-1
chr8_1 CDS 27597780 27598304 27597780 ID=Msa1187720-mRNA-1:cds;Parent=Msa1187720-mRNA-1
chr8_1 CDS 27598390 27599463 27598390 ID=Msa1187720-mRNA-1:cds;Parent=Msa1187720-mRNA-1
Gene Sequence

>Msa1187720

ATGGAAAACTTAACAGAGCTTCGTATAATCGGAAGTCCTATAAAAGAATTGCCACCTTCAATTCAAAACCTTAATCGGCTTCGAAAATTAGAACTACAAATCTGTGGAATGGTTCAGTTACCAAGTAGCATTGCCATGTTTTCACAACTTGGTTTGATGTGTGTTTCGAGATGTGAAGGGTTGTGGTTATCTAAACCGGACATAAGTGAAGAATGGGAGACCAAGTCTTCGAAGACAGAATTTCTTATTCTGTCCTACTGCAATTTATCAGATGATTTCCTTCCCATAGGTCTGTCTTGGTTTGCCAATGTGAAAGATTTGGACCTATCCGGGAATAATTTCACTATTCTTCACGCATGCATCAAAGAGTGTCACTTTCTCAGGAACCTAAAGATAGATGACTGCGTGCATATACGAGAAATTACAGTGATACCATGCAAATTAGAAACACTTTCTGCAAAACGATGCAAATCCTTGAAATACATAGACCTTACAGAAGAATGTCAATCTTTGAGAGAACTTATTCTGGACGACTGTATTTATCTTCGAGAAATTGACAGGGTTCTGCCAAACTTGGATCATTTCTCTGCCAAAAACTGCACTTTATTGACTTCTCAGTGTGCAAGCTTGTTAGTGAATCAGGAAATGATTGAAGCCGGAAACAAGATGTTTTCTTTGCCAGGAACAAAGATTCCAGAGTGGTTTTCACACCGTACAAGTGGAGATTCAATATCTTTCTGGTTTCGTAACAGATTCCCTGCAATTTGTCTGTGCCTTGTTATTGGGCAGGCGGATGAGCTACCTATCTCAGTCAAGTTTAGCCCCAAAGTGTTCCTCAATGGCAACGAACAGTCCAGTGGCAATCAGGTAGTCTACAAGTTCAGGATAGCTACTGATCATATACTTTTGTTTGATGGGCAGATGCTAAAATTTGAAGATAACGGAGAAATAGTGTTTTCAGACAATGAGTGGAATCATGTGGTGGTTTCATATGAGGATCTTATTACCAACAATGGGGTTCCAATCAAAGTGGTTGCCAAATATAATGGAATCCATGTATCTCAACAAAGTAGTATTGAGAATATCCGATTCAGCAATCCCCAACAAACATTGGTAAATGTGAATTTCAATCCCGATTCAATCGTGGGGTCACCTCAAAGAGTAAAGATTATGACTAAGAGACCCCAGGAAGACCAAACCACTGTGCTGTCTCCCTCACTCACATCAACACAATCTCCTTCTCCACCCTCTGAAATCAAAAGAGGCATTGTCACTCTTCCTACATTTCCAGTAAAGATGCCTTTGGGAGATGTGGGAAGAGAAAGTTCAGAGAATCTTTCCGATAATGAAGCTTATGCTACTGCAATTCAAAATGAGACTCCTCTCATTCAAAGATGTAGTGAGGGTGATGATGTCGAGCTGGAATTAGTATCCTGTGAAGAAGAGTCTTCTTCCAACACCGAAGGTTCTGACTCGGATGATCCCTTCGACTTAGTTAATAGAAAACTATGCGTTAGTGGCAAGAAAACCATATCTTCTGGGGTAAGTTCTGGAGATGCAAATCTAAGATCTATTAGAGAGACTATTAATTCACTGGAGTTATTGATGATTAAGGACTTATCTGAAGTCTCTGCTGATCCTTCCGCACATTCCAAACTTCGTCAGCTCTTGGATCTATTGAGTGCAAGTAGCCACCCCAAGGTGACAATAGAGATGAAGGAGGCTATTGCTGAGTTCAAGAGAAAGGCCTTTGCATCATTCCATGAATTCCAAGCCACTGTTGAATCTGTGAGTAAGCTGAAGAATTATGAGAATCATCTGGCCAGAATTCAGCACGAGACTGTGGCCGGCAAGGATCAATGGAAGGACTTCAAAAAATCAATAAAGAAGGTTTCTTTGTACATCAAGGCTGAAAATAGTAGGAAAAAGGAACTAGAGTCAGAAATTGCAACTTTAAGGAAACAGCTAGCTTCCAAAGAAAAGGACCTCGAACAACTTGTGTTGAATCTCAAGAATCAGGAGACAACACTCTCAACTTATTCAACGAGTTATGCTTCTCTCAATGAACATGCTCGAGCACTATCAGAAGAAGCTGATGATCTACTTGCTTCAAGCGGTGAGATAAAACATGATGGCGAAGCAGCTGAAATAGAGCAGAGTAGGCTCAAGTGGACTTGGTCTATTGACCTAACCGGCCAGCTTAATAAGATGAAAGAAAACATTCTTGGCTCATATGAGTAG
Protein sequence

>Msa1187720

MENLTELRIIGSPIKELPPSIQNLNRLRKLELQICGMVQLPSSIAMFSQLGLMCVSRCEGLWLSKPDISEEWETKSSKTEFLILSYCNLSDDFLPIGLSWFANVKDLDLSGNNFTILHACIKECHFLRNLKIDDCVHIREITVIPCKLETLSAKRCKSLKYIDLTEECQSLRELILDDCIYLREIDRVLPNLDHFSAKNCTLLTSQCASLLVNQEMIEAGNKMFSLPGTKIPEWFSHRTSGDSISFWFRNRFPAICLCLVIGQADELPISVKFSPKVFLNGNEQSSGNQVVYKFRIATDHILLFDGQMLKFEDNGEIVFSDNEWNHVVVSYEDLITNNGVPIKVVAKYNGIHVSQQSSIENIRFSNPQQTLVNVNFNPDSIVGSPQRVKIMTKRPQEDQTTVLSPSLTSTQSPSPPSEIKRGIVTLPTFPVKMPLGDVGRESSENLSDNEAYATAIQNETPLIQRCSEGDDVELELVSCEEESSSNTEGSDSDDPFDLVNRKLCVSGKKTISSGVSSGDANLRSIRETINSLELLMIKDLSEVSADPSAHSKLRQLLDLLSASSHPKVTIEMKEAIAEFKRKAFASFHEFQATVESVSKLKNYENHLARIQHETVAGKDQWKDFKKSIKKVSLYIKAENSRKKELESEIATLRKQLASKEKDLEQLVLNLKNQETTLSTYSTSYASLNEHARALSEEADDLLASSGEIKHDGEAAEIEQSRLKWTWSIDLTGQLNKMKENILGSYE*