AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1286000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1286000 MtrunA17_Chr2g0289091 64.205 176 16 3 1 136 252 420 3.32e-64 202
Msa1286000 MtrunA17_Chr2g0289121 70.833 144 8 1 44 153 658 801 2.66e-60 199
Msa1286000 MtrunA17_Chr2g0289031 63.571 140 17 1 48 153 236 375 4.35e-52 170
Msa1286000 MtrunA17_Chr4g0075261 61.654 133 17 1 55 153 714 846 3.23e-44 154
Msa1286000 MtrunA17_Chr1g0183311 54.478 134 27 1 54 153 728 861 5.55e-40 142
Msa1286000 MtrunA17_Chr1g0211711 52.518 139 31 2 50 153 892 1030 6.98e-39 139
Msa1286000 MtrunA17_Chr8g0361391 62.963 108 7 1 40 114 30 137 7.22e-38 127
Msa1286000 MtrunA17_Chr5g0426961 52.985 134 29 1 54 153 681 814 8.17e-38 136
Msa1286000 MtrunA17_Chr5g0420361 54.688 128 24 1 60 153 650 777 4.56e-37 134
Msa1286000 MtrunA17_Chr6g0472441 52.344 128 27 1 60 153 572 699 3.96e-35 128
Msa1286000 MtrunA17_Chr7g0275771 53.125 128 26 1 60 153 779 906 5.95e-35 128
Msa1286000 MtrunA17_Chr1g0147021 50.394 127 29 1 61 153 1551 1677 3.80e-32 120
Msa1286000 MtrunA17_Chr1g0211701 70.492 61 18 0 93 153 37 97 9.08e-26 95.9
Msa1286000 MtrunA17_Chr4g0048531 38.168 131 48 2 56 153 1128 1258 5.84e-23 94.0
Msa1286000 MtrunA17_Chr1g0192011 29.143 175 91 3 8 149 764 938 1.75e-20 87.0
Msa1286000 MtrunA17_Chr2g0281051 34.646 127 49 2 61 153 810 936 2.73e-18 80.5
Msa1286000 MtrunA17_Chr1g0211691 54.902 51 23 0 46 96 895 945 1.23e-12 64.3
Msa1286000 MtrunA17_Chr1g0211671 64.103 39 14 0 115 153 17 55 3.93e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1286000 AT1G62310.1 58.156 141 24 2 48 153 736 876 1.18e-44 156
Msa1286000 AT1G11950.1 59.542 131 19 1 57 153 720 850 3.96e-43 152
Msa1286000 AT1G11950.3 59.542 131 19 1 57 153 720 850 3.96e-43 152
Msa1286000 AT3G07610.1 51.562 128 28 1 60 153 746 873 1.80e-35 130
Msa1286000 AT3G07610.3 51.562 128 28 1 60 153 768 895 2.18e-35 129
Msa1286000 AT4G00990.1 44.828 145 44 2 45 153 667 811 7.55e-31 117
Msa1286000 AT1G09060.2 34.400 125 52 2 55 149 744 868 4.50e-16 74.7
Msa1286000 AT1G09060.1 34.400 125 52 2 55 149 744 868 4.50e-16 74.7
Msa1286000 AT1G09060.4 35.000 120 48 2 60 149 763 882 4.60e-16 74.7
Msa1286000 AT1G09060.3 35.000 120 48 2 60 149 763 882 4.60e-16 74.7
Msa1286000 AT1G11950.2 45.122 82 11 1 57 104 720 801 1.83e-12 64.3

Find 38 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAATATTCCAAAGAATTT+AGG 0.204576 8_3:-32582922 Msa1286000:CDS
ATGAAGGCCCTTCCATTTCT+TGG 0.236275 8_3:+32584312 None:intergenic
ATAAGTATGCCTAAATTCTT+TGG 0.239219 8_3:+32582913 None:intergenic
TCTCTTGCCCTGTGATTCTT+AGG 0.277632 8_3:+32581437 None:intergenic
GAGGGTCTTGTTCAAGATAA+TGG 0.316458 8_3:-32583080 Msa1286000:CDS
GTGATTGGAGGAACTTCATT+TGG 0.317060 8_3:+32583025 None:intergenic
TCTGATGTACCTTCTGTGAT+TGG 0.338412 8_3:+32583010 None:intergenic
TTCCATTTCTTGGATTTCCT+TGG 0.346100 8_3:+32584322 None:intergenic
AGAAACAGTTGGTGCACTTT+GGG 0.351100 8_3:-32582990 Msa1286000:CDS
AAGAAATGGAAGGGCCTTCA+TGG 0.363671 8_3:-32584310 Msa1286000:CDS
TCACCTTCAGATTTCGAACT+TGG 0.382940 8_3:+32581665 None:intergenic
CAGAAACAGTTGGTGCACTT+TGG 0.398016 8_3:-32582991 Msa1286000:CDS
CACCAAGGAAATCCAAGAAA+TGG 0.417916 8_3:-32584324 Msa1286000:CDS
TGATTGGAGGAACTTCATTT+GGG 0.423722 8_3:+32583026 None:intergenic
AGCTTTCCTTTGATCTCAAA+AGG 0.429428 8_3:+32583053 None:intergenic
CACCTTCAGATTTCGAACTT+GGG 0.457793 8_3:+32581666 None:intergenic
TTATTGTTCTCCAGTAAAAG+AGG 0.483787 8_3:-32582894 Msa1286000:intron
GTGTCTACAATTGCATCTGC+TGG 0.487941 8_3:+32584344 None:intergenic
GTCGCCTTCCTAAGAATCAC+AGG 0.498370 8_3:-32581445 Msa1286000:CDS
TTGCCCTGTGATTCTTAGGA+AGG 0.529013 8_3:+32581441 None:intergenic
ACCTTCAGATTTCGAACTTG+GGG 0.534211 8_3:+32581667 None:intergenic
GATGTACCTTCTGTGATTGG+AGG 0.535773 8_3:+32583013 None:intergenic
CTTTGTGTCACCAGAAAATG+TGG 0.546636 8_3:-32581498 Msa1286000:CDS
ACTTGGGGAGGGCATCCAGC+AGG 0.559265 8_3:+32581682 None:intergenic
TTCAGATTTCGAACTTGGGG+AGG 0.560279 8_3:+32581670 None:intergenic
AACGTCGGCATTTGATCAAG+CGG 0.578329 8_3:-32584390 Msa1286000:five_prime_UTR
TCAGATTTCGAACTTGGGGA+GGG 0.580132 8_3:+32581671 None:intergenic
AAGGAAATCCAAGAAATGGA+AGG 0.594356 8_3:-32584320 Msa1286000:CDS
TCCCCAAGTTCGAAATCTGA+AGG 0.601439 8_3:-32581668 Msa1286000:intron
GAAGCAGTATTTATACCTGC+TGG 0.606997 8_3:-32581697 Msa1286000:CDS
ATGGAAAGTGAGAAAAGAAG+AGG 0.612198 8_3:-32584291 Msa1286000:intron
GAAGTTCCTCCAATCACAGA+AGG 0.616489 8_3:-32583019 Msa1286000:CDS
AGGAAATCCAAGAAATGGAA+GGG 0.618012 8_3:-32584319 Msa1286000:CDS
GAAGGTACATCAGAAACAGT+TGG 0.648315 8_3:-32583001 Msa1286000:CDS
TCAAGCGGATGATACAAGAC+AGG 0.650009 8_3:-32584375 Msa1286000:five_prime_UTR
CTGTCATTGGAGTTCAACGT+CGG 0.678503 8_3:-32584405 None:intergenic
AGATGCAATTGTAGACACCA+AGG 0.690581 8_3:-32584339 Msa1286000:CDS
TCGCCTTCCTAAGAATCACA+GGG 0.703131 8_3:-32581444 Msa1286000:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTCATTTCTGAAAATTA+TGG - chr8_3:32581819-32581838 Msa1286000:intron 15.0%
!!! GAAATTAATAAAAAGCATAA+TGG - chr8_3:32581841-32581860 Msa1286000:intron 15.0%
!! TATAATAAAAATTGACACAT+GGG + chr8_3:32583035-32583054 None:intergenic 15.0%
!! ATATAATAAAAATTGACACA+TGG + chr8_3:32583036-32583055 None:intergenic 15.0%
!!! AGTAACATTTTATTTTATAG+AGG + chr8_3:32583705-32583724 None:intergenic 15.0%
!!! TTTTTTTAACAAGGTTTATA+TGG - chr8_3:32583894-32583913 Msa1286000:intron 15.0%
!!! TTTTTTAACAAGGTTTATAT+GGG - chr8_3:32583895-32583914 Msa1286000:intron 15.0%
!!! GTACTTTTAAACTTAAATTT+AGG + chr8_3:32584209-32584228 None:intergenic 15.0%
!!! TGATCTAAAATAGCTTTTAA+AGG + chr8_3:32581799-32581818 None:intergenic 20.0%
!!! ATTCATTTGAAATATTTGCA+AGG - chr8_3:32581877-32581896 Msa1286000:intron 20.0%
!! ATCATCCAATAAAGTAAAAA+TGG - chr8_3:32582001-32582020 Msa1286000:intron 20.0%
!! GAAAATATTCCAAAGAATTT+AGG - chr8_3:32582898-32582917 Msa1286000:CDS 20.0%
!!! TAATATAATACCTCTTTTAC+TGG + chr8_3:32582939-32582958 None:intergenic 20.0%
!! ATCACATTTCATCAATATTT+CGG - chr8_3:32582958-32582977 Msa1286000:CDS 20.0%
!! TATTTCGGCTTATATTTATT+TGG - chr8_3:32582973-32582992 Msa1286000:CDS 20.0%
!! ATTTCGGCTTATATTTATTT+GGG - chr8_3:32582974-32582993 Msa1286000:CDS 20.0%
!!! ATATTTATTTGGGTTATGTT+TGG - chr8_3:32582984-32583003 Msa1286000:CDS 20.0%
!!! ATACTGAGTTTTTTTTAACA+AGG - chr8_3:32583885-32583904 Msa1286000:intron 20.0%
!!! AATTAATCTAGAAACAGTTT+TGG + chr8_3:32584021-32584040 None:intergenic 20.0%
!! AAACTTAAATTTAGGTAAAG+AGG + chr8_3:32584201-32584220 None:intergenic 20.0%
! ATCATCCAATAAAGTAAAAC+TGG + chr8_3:32581768-32581787 None:intergenic 25.0%
!!! CTTTGCCATTTTTACTTTAT+TGG + chr8_3:32582009-32582028 None:intergenic 25.0%
! ATAAGTATGCCTAAATTCTT+TGG + chr8_3:32582910-32582929 None:intergenic 25.0%
!! CAAAACCTAGAATTTAAGAT+CGG + chr8_3:32583570-32583589 None:intergenic 25.0%
! CAAGAATCTTAATCAATTAC+AGG - chr8_3:32584066-32584085 Msa1286000:intron 25.0%
TGTTATCAAATTGTACTCCA+AGG - chr8_3:32581680-32581699 Msa1286000:CDS 30.0%
! CTTTGCCAGTTTTACTTTAT+TGG - chr8_3:32581760-32581779 Msa1286000:intron 30.0%
!!! CTGAGTTTTAAAACAAGGTT+AGG + chr8_3:32581965-32581984 None:intergenic 30.0%
!!! TTGCTCTGAGTTTTAAAACA+AGG + chr8_3:32581970-32581989 None:intergenic 30.0%
!!! TGTTTTAAAACTCAGAGCAA+AGG - chr8_3:32581970-32581989 Msa1286000:intron 30.0%
TGATATCAAATTGTACTGCA+AGG + chr8_3:32582089-32582108 None:intergenic 30.0%
! TTTTGTATATCTTGCGCTAA+CGG + chr8_3:32581637-32581656 None:intergenic 30.0%
ACATTTAGCATTTGATCAAG+CGG + chr8_3:32582339-32582358 None:intergenic 30.0%
! GAAAAGCTAGTTGATTCTAT+GGG + chr8_3:32582428-32582447 None:intergenic 30.0%
! TGAAAAGCTAGTTGATTCTA+TGG + chr8_3:32582429-32582448 None:intergenic 30.0%
CACATCATTATTGAAACCTT+CGG - chr8_3:32582483-32582502 Msa1286000:intron 30.0%
ATTGAAGTCAGTTGAAAAAC+CGG - chr8_3:32582522-32582541 Msa1286000:intron 30.0%
!! TGAATGTTGATTTCGAACAT+CGG + chr8_3:32582621-32582640 None:intergenic 30.0%
TGTAAACCATTGTCAATAAC+TGG - chr8_3:32582709-32582728 Msa1286000:intron 30.0%
TTATTGTTCTCCAGTAAAAG+AGG - chr8_3:32582926-32582945 Msa1286000:CDS 30.0%
AATAAAAATTGACACATGGG+TGG + chr8_3:32583032-32583051 None:intergenic 30.0%
TTGAATTACAGTAGATCTGA+GGG + chr8_3:32583140-32583159 None:intergenic 30.0%
AGTTTCCGATCTTAAATTCT+AGG - chr8_3:32583562-32583581 Msa1286000:intron 30.0%
!! AGTTTGATGAACTGTTTCTA+CGG - chr8_3:32583748-32583767 Msa1286000:intron 30.0%
! GGTATTTCATCTTTTGATGA+AGG + chr8_3:32583936-32583955 None:intergenic 30.0%
AAAACTATTCTGCCACATAA+GGG - chr8_3:32583956-32583975 Msa1286000:intron 30.0%
! ATGCATATATCCACATTTTC+TGG + chr8_3:32584335-32584354 None:intergenic 30.0%
TTCCATTTCTTGGATTTCCT+TGG + chr8_3:32581501-32581520 None:intergenic 35.0%
AAGGAAATCCAAGAAATGGA+AGG - chr8_3:32581500-32581519 Msa1286000:CDS 35.0%
AGGAAATCCAAGAAATGGAA+GGG - chr8_3:32581501-32581520 Msa1286000:CDS 35.0%
! TCTTTTCTCACTTTCCATGA+AGG + chr8_3:32581527-32581546 None:intergenic 35.0%
ATGGAAAGTGAGAAAAGAAG+AGG - chr8_3:32581529-32581548 Msa1286000:intron 35.0%
!! TGCAGACTGAGTTTTAATCT+TGG + chr8_3:32581932-32581951 None:intergenic 35.0%
ATGGAAAGTGAGAAAAGAAG+AGG + chr8_3:32581529-32581548 None:intergenic 35.0%
TATCAAAGAAGACAGTCTTG+AGG + chr8_3:32582378-32582397 None:intergenic 35.0%
! TAGTTGATTCTATGGGACAA+AGG + chr8_3:32582421-32582440 None:intergenic 35.0%
AGGTTTCAATAATGATGTGG+AGG + chr8_3:32582482-32582501 None:intergenic 35.0%
CGAAGGTTTCAATAATGATG+TGG + chr8_3:32582485-32582504 None:intergenic 35.0%
AATTTCAAAAGTTCTGCCGA+AGG + chr8_3:32582502-32582521 None:intergenic 35.0%
GTCAAAATGGTGACTTAGTT+AGG + chr8_3:32582576-32582595 None:intergenic 35.0%
ATCAACATTCACTTCATCGA+TGG - chr8_3:32582630-32582649 Msa1286000:intron 35.0%
TCAACATTCACTTCATCGAT+GGG - chr8_3:32582631-32582650 Msa1286000:intron 35.0%
TTTAACGTTAGGTTCCCATT+TGG + chr8_3:32582661-32582680 None:intergenic 35.0%
GTGTTGCAAACTTTAACGTT+AGG + chr8_3:32582672-32582691 None:intergenic 35.0%
TCTGAACCAGTTATTGACAA+TGG + chr8_3:32582718-32582737 None:intergenic 35.0%
! TCAATAACTGGTTCAGAAAG+AGG - chr8_3:32582721-32582740 Msa1286000:intron 35.0%
! CAATAACTGGTTCAGAAAGA+GGG - chr8_3:32582722-32582741 Msa1286000:intron 35.0%
AGCTTTCCTTTGATCTCAAA+AGG + chr8_3:32582770-32582789 None:intergenic 35.0%
TGATTGGAGGAACTTCATTT+GGG + chr8_3:32582797-32582816 None:intergenic 35.0%
ATGGGTGGTATGGAAATTAA+AGG + chr8_3:32583017-32583036 None:intergenic 35.0%
GAAAAAATACCGTAACTCGT+CGG - chr8_3:32583103-32583122 Msa1286000:intron 35.0%
GTTGAATTACAGTAGATCTG+AGG + chr8_3:32583141-32583160 None:intergenic 35.0%
ATCATTGAAAGAGGAAAGAG+CGG + chr8_3:32583384-32583403 None:intergenic 35.0%
GATAGGTTCATCATTGAAAG+AGG + chr8_3:32583393-32583412 None:intergenic 35.0%
!! GTTTGATGAACTGTTTCTAC+GGG - chr8_3:32583749-32583768 Msa1286000:intron 35.0%
! CTTATGTGGCAGAATAGTTT+TGG + chr8_3:32583957-32583976 None:intergenic 35.0%
CAAAACTATTCTGCCACATA+AGG - chr8_3:32583955-32583974 Msa1286000:intron 35.0%
AAACTATTCTGCCACATAAG+GGG - chr8_3:32583957-32583976 Msa1286000:intron 35.0%
AAGATTGTGTTTCCAGCTAT+GGG - chr8_3:32583985-32584004 Msa1286000:intron 35.0%
AGATGCAATTGTAGACACCA+AGG - chr8_3:32581481-32581500 Msa1286000:CDS 40.0%
CACCAAGGAAATCCAAGAAA+TGG - chr8_3:32581496-32581515 Msa1286000:CDS 40.0%
AATTGTACTCCAAGGAGTCA+TGG - chr8_3:32581688-32581707 Msa1286000:CDS 40.0%
AGATGTTAACCATGACTCCT+TGG + chr8_3:32581700-32581719 None:intergenic 40.0%
AACTCAGTCTGCATACATTG+TGG - chr8_3:32581939-32581958 Msa1286000:intron 40.0%
TTACCGCTGTAACTTTCATG+CGG + chr8_3:32581720-32581739 None:intergenic 40.0%
AATTGTACTGCAAGGAGTCA+TGG + chr8_3:32582081-32582100 None:intergenic 40.0%
! TCTTTTCTCACTTTCCATGC+AGG - chr8_3:32582242-32582261 Msa1286000:intron 40.0%
GGGAAATCCAAGAAATGGAA+GGG + chr8_3:32582268-32582287 None:intergenic 40.0%
AGGGAAATCCAAGAAATGGA+AGG + chr8_3:32582269-32582288 None:intergenic 40.0%
TTCCATTTCTTGGATTTCCC+TGG - chr8_3:32582268-32582287 Msa1286000:intron 40.0%
TCAAGCGGATGATAGAAGAT+AGG + chr8_3:32582324-32582343 None:intergenic 40.0%
CTCAGAAACACAGAAGTTAC+CGG + chr8_3:32582544-32582563 None:intergenic 40.0%
! ACTTCTGTGTTTCTGAGTAG+TGG - chr8_3:32582547-32582566 Msa1286000:intron 40.0%
!! GAGGGTCTTGTTCAAGATAA+TGG - chr8_3:32582740-32582759 Msa1286000:intron 40.0%
!!! GGAAGTCCTTTTGAGATCAA+AGG - chr8_3:32582761-32582780 Msa1286000:intron 40.0%
GTGATTGGAGGAACTTCATT+TGG + chr8_3:32582798-32582817 None:intergenic 40.0%
TCTGATGTACCTTCTGTGAT+TGG + chr8_3:32582813-32582832 None:intergenic 40.0%
! GAAGGTACATCAGAAACAGT+TGG - chr8_3:32582819-32582838 Msa1286000:intron 40.0%
! AGAAACAGTTGGTGCACTTT+GGG - chr8_3:32582830-32582849 Msa1286000:intron 40.0%
AAATTGACACATGGGTGGTA+TGG + chr8_3:32583027-32583046 None:intergenic 40.0%
TAGATCTGAGGGCAATGAAA+AGG + chr8_3:32583129-32583148 None:intergenic 40.0%
ACAAATTCACGGCTAACTGA+GGG + chr8_3:32583464-32583483 None:intergenic 40.0%
AAACCCTAGCGACAAATTCA+CGG + chr8_3:32583475-32583494 None:intergenic 40.0%
!! GTTTCAGGTTGTGTTTCTTC+TGG - chr8_3:32583491-32583510 Msa1286000:intron 40.0%
TGTGTTTCTTCTGGTGCTTT+AGG - chr8_3:32583500-32583519 Msa1286000:intron 40.0%
! TTTCTTCTGGTGCTTTAGGT+TGG - chr8_3:32583504-32583523 Msa1286000:intron 40.0%
GGTTCTTCTGAATTGTGAGA+AGG - chr8_3:32583770-32583789 Msa1286000:intron 40.0%
TCTTCTGAATTGTGAGAAGG+AGG - chr8_3:32583773-32583792 Msa1286000:intron 40.0%
AATCTTGTACACCCCTTATG+TGG + chr8_3:32583971-32583990 None:intergenic 40.0%
CAAGATTGTGTTTCCAGCTA+TGG - chr8_3:32583984-32584003 Msa1286000:intron 40.0%
CAATTACAGGTGTAGTACCT+TGG - chr8_3:32584079-32584098 Msa1286000:intron 40.0%
GAAGCAGTATTTATACCTGC+TGG - chr8_3:32584123-32584142 Msa1286000:intron 40.0%
ACCTTCAGATTTCGAACTTG+GGG + chr8_3:32584156-32584175 None:intergenic 40.0%
CACCTTCAGATTTCGAACTT+GGG + chr8_3:32584157-32584176 None:intergenic 40.0%
TCACCTTCAGATTTCGAACT+TGG + chr8_3:32584158-32584177 None:intergenic 40.0%
CTTTGTGTCACCAGAAAATG+TGG - chr8_3:32584322-32584341 Msa1286000:CDS 40.0%
AACGTCGGCATTTGATCAAG+CGG - chr8_3:32581430-32581449 Msa1286000:CDS 45.0%
TCAAGCGGATGATACAAGAC+AGG - chr8_3:32581445-32581464 Msa1286000:CDS 45.0%
GTGTCTACAATTGCATCTGC+TGG + chr8_3:32581479-32581498 None:intergenic 45.0%
ATGAAGGCCCTTCCATTTCT+TGG + chr8_3:32581511-32581530 None:intergenic 45.0%
AAGAAATGGAAGGGCCTTCA+TGG - chr8_3:32581510-32581529 Msa1286000:CDS 45.0%
ACGCTGCATGAAAGTTACAG+CGG + chr8_3:32581726-32581745 None:intergenic 45.0%
ACACCGCATGAAAGTTACAG+CGG - chr8_3:32582043-32582062 Msa1286000:intron 45.0%
ATGTATGCCAGCAGATGCAA+TGG + chr8_3:32582300-32582319 None:intergenic 45.0%
!! TAAGTCACCATTTTGACCCC+AGG - chr8_3:32582579-32582598 Msa1286000:intron 45.0%
!! ACCATTTTGACCCCAGGAAT+CGG - chr8_3:32582585-32582604 Msa1286000:intron 45.0%
GAAGTTCCTCCAATCACAGA+AGG - chr8_3:32582801-32582820 Msa1286000:intron 45.0%
GATGTACCTTCTGTGATTGG+AGG + chr8_3:32582810-32582829 None:intergenic 45.0%
! CAGAAACAGTTGGTGCACTT+TGG - chr8_3:32582829-32582848 Msa1286000:intron 45.0%
ATGAAAAGGCCGACGAGTTA+CGG + chr8_3:32583115-32583134 None:intergenic 45.0%
TGAGGTTAGAGAAACACACG+AGG + chr8_3:32583229-32583248 None:intergenic 45.0%
CATGGATAGTTGGAGAGGAA+GGG + chr8_3:32583259-32583278 None:intergenic 45.0%
ACATGGATAGTTGGAGAGGA+AGG + chr8_3:32583260-32583279 None:intergenic 45.0%
AGAGACATGGATAGTTGGAG+AGG + chr8_3:32583264-32583283 None:intergenic 45.0%
GCGTAAGAGACATGGATAGT+TGG + chr8_3:32583269-32583288 None:intergenic 45.0%
! GTAAGAGAGCGTAAGAGACA+TGG + chr8_3:32583277-32583296 None:intergenic 45.0%
GGAGTGAAGGAATTGAGGAA+TGG + chr8_3:32583318-32583337 None:intergenic 45.0%
ACGATGGAGTGAAGGAATTG+AGG + chr8_3:32583323-32583342 None:intergenic 45.0%
GAAAACTGACGATGGAGTGA+AGG + chr8_3:32583331-32583350 None:intergenic 45.0%
GAGGTGAAACAAGGAACTGA+GGG + chr8_3:32583355-32583374 None:intergenic 45.0%
AGAGGTGAAACAAGGAACTG+AGG + chr8_3:32583356-32583375 None:intergenic 45.0%
CTAACGAGTTGTTCTGCGAT+AGG + chr8_3:32583410-32583429 None:intergenic 45.0%
GACAAATTCACGGCTAACTG+AGG + chr8_3:32583465-32583484 None:intergenic 45.0%
TTAGCCGTGAATTTGTCGCT+AGG - chr8_3:32583468-32583487 Msa1286000:intron 45.0%
TAGCCGTGAATTTGTCGCTA+GGG - chr8_3:32583469-32583488 Msa1286000:intron 45.0%
GAATTTGTCGCTAGGGTTTC+AGG - chr8_3:32583476-32583495 Msa1286000:intron 45.0%
AGCTTCAGCTCAAAAGTCCA+AGG + chr8_3:32584099-32584118 None:intergenic 45.0%
! TGGACTTTTGAGCTGAAGCT+TGG - chr8_3:32584099-32584118 Msa1286000:intron 45.0%
TCAGATTTCGAACTTGGGGA+GGG + chr8_3:32584152-32584171 None:intergenic 45.0%
TTCAGATTTCGAACTTGGGG+AGG + chr8_3:32584153-32584172 None:intergenic 45.0%
TCCCCAAGTTCGAAATCTGA+AGG - chr8_3:32584152-32584171 Msa1286000:intron 45.0%
TCGCCTTCCTAAGAATCACA+GGG - chr8_3:32584376-32584395 Msa1286000:five_prime_UTR 45.0%
TTGCCCTGTGATTCTTAGGA+AGG + chr8_3:32584382-32584401 None:intergenic 45.0%
TCTCTTGCCCTGTGATTCTT+AGG + chr8_3:32584386-32584405 None:intergenic 45.0%
AAGAAATGGAAGGGCCTGCA+TGG + chr8_3:32582259-32582278 None:intergenic 50.0%
ATGCAGGCCCTTCCATTTCT+TGG - chr8_3:32582258-32582277 Msa1286000:intron 50.0%
CGCCAGGGAAATCCAAGAAA+TGG + chr8_3:32582273-32582292 None:intergenic 50.0%
AGATGCAATGGTAGACGCCA+GGG + chr8_3:32582288-32582307 None:intergenic 50.0%
GACAGTCTTGAGGCTGTCAT+TGG + chr8_3:32582368-32582387 None:intergenic 50.0%
ATGGGACAAAGGCTTGCAAG+TGG + chr8_3:32582410-32582429 None:intergenic 50.0%
! ACCGATTCCTGGGGTCAAAA+TGG + chr8_3:32582589-32582608 None:intergenic 50.0%
TGACCCCAGGAATCGGTATT+TGG - chr8_3:32582592-32582611 Msa1286000:intron 50.0%
GACCCCAGGAATCGGTATTT+GGG - chr8_3:32582593-32582612 Msa1286000:intron 50.0%
ACCCCAGGAATCGGTATTTG+GGG - chr8_3:32582594-32582613 Msa1286000:intron 50.0%
!! TCATCGATGGGAGCACCAAA+TGG - chr8_3:32582643-32582662 Msa1286000:intron 50.0%
!! CATCGATGGGAGCACCAAAT+GGG - chr8_3:32582644-32582663 Msa1286000:intron 50.0%
GAGAGGAAGGGTTAGAGATG+AGG + chr8_3:32583247-32583266 None:intergenic 50.0%
CTGAGGGAGAAAACTGACGA+TGG + chr8_3:32583339-32583358 None:intergenic 50.0%
CGGCAATGAGAGGTGAAACA+AGG + chr8_3:32583364-32583383 None:intergenic 50.0%
TTCACGGCTAACTGAGGGAT+CGG + chr8_3:32583459-32583478 None:intergenic 50.0%
GTGAGAAGGAGGATGAGAAG+AGG - chr8_3:32583784-32583803 Msa1286000:intron 50.0%
AGGATGAGAAGAGGAGTGAG+CGG - chr8_3:32583793-32583812 Msa1286000:intron 50.0%
GGTAAGTGAACACCCATAGC+TGG + chr8_3:32584000-32584019 None:intergenic 50.0%
GTCGCCTTCCTAAGAATCAC+AGG - chr8_3:32584375-32584394 Msa1286000:five_prime_UTR 50.0%
CAGATGCAATGGTAGACGCC+AGG + chr8_3:32582289-32582308 None:intergenic 55.0%
GCGTCTACCATTGCATCTGC+TGG - chr8_3:32582290-32582309 Msa1286000:intron 55.0%
!! CCCCCAAATACCGATTCCTG+GGG + chr8_3:32582598-32582617 None:intergenic 55.0%
CCCCAGGAATCGGTATTTGG+GGG - chr8_3:32582595-32582614 Msa1286000:intron 55.0%
!! GCCCCCAAATACCGATTCCT+GGG + chr8_3:32582599-32582618 None:intergenic 55.0%
GGTGGCAGCAACAGTGTTTG+TGG + chr8_3:32583163-32583182 None:intergenic 55.0%
GAGGAAAGAGCGGCAATGAG+AGG + chr8_3:32583374-32583393 None:intergenic 55.0%
ATGAGAAGAGGAGTGAGCGG+CGG - chr8_3:32583796-32583815 Msa1286000:intron 55.0%
!! GGCCCCCAAATACCGATTCC+TGG + chr8_3:32582600-32582619 None:intergenic 60.0%
CGGAGGAAGCAGAGAGGTTG+AGG + chr8_3:32583439-32583458 None:intergenic 60.0%
AGGGATCGGAGGAAGCAGAG+AGG + chr8_3:32583445-32583464 None:intergenic 60.0%
ACGGCTAACTGAGGGATCGG+AGG + chr8_3:32583456-32583475 None:intergenic 60.0%
AAGGAGAGAGGGGGCGATGA+TGG - chr8_3:32583831-32583850 Msa1286000:intron 60.0%
!! CCTTGGTTTGTGCGCCCGGA+GGG + chr8_3:32583194-32583213 None:intergenic 65.0%
!! GCTGCCTTGGTTTGTGCGCC+CGG + chr8_3:32583198-32583217 None:intergenic 65.0%
CGAGGCAGATCGTGCTGCCT+TGG + chr8_3:32583211-32583230 None:intergenic 65.0%
GAAGAGGAGTGAGCGGCGGA+CGG - chr8_3:32583800-32583819 Msa1286000:intron 65.0%
ACGGCGACGGTGAAGGAGAG+AGG - chr8_3:32583819-32583838 Msa1286000:intron 65.0%
CGGCGACGGTGAAGGAGAGA+GGG - chr8_3:32583820-32583839 Msa1286000:intron 65.0%
GGCGACGGTGAAGGAGAGAG+GGG - chr8_3:32583821-32583840 Msa1286000:intron 65.0%
GCGACGGTGAAGGAGAGAGG+GGG - chr8_3:32583822-32583841 Msa1286000:intron 65.0%
! GAGAGAGGGGGCGATGATGG+TGG - chr8_3:32583834-32583853 Msa1286000:intron 65.0%
!! AGAGGGGGCGATGATGGTGG+AGG - chr8_3:32583837-32583856 Msa1286000:intron 65.0%
!! ACTTGGGGAGGGCATCCAGC+AGG + chr8_3:32584141-32584160 None:intergenic 65.0%
GGTTTGTGCGCCCGGAGGGA+CGG + chr8_3:32583190-32583209 None:intergenic 70.0%
CCCTCCGGGCGCACAAACCA+AGG - chr8_3:32583191-32583210 Msa1286000:intron 70.0%
!! GCCTTGGTTTGTGCGCCCGG+AGG + chr8_3:32583195-32583214 None:intergenic 70.0%
TGCCACCGCTGCCGTCCCTC+CGG - chr8_3:32583176-32583195 Msa1286000:intron 75.0%
GAGTGAGCGGCGGACGGCGA+CGG - chr8_3:32583806-32583825 Msa1286000:intron 75.0%
! GCCACCGCTGCCGTCCCTCC+GGG - chr8_3:32583177-32583196 Msa1286000:intron 80.0%
! TGCGCCCGGAGGGACGGCAG+CGG + chr8_3:32583184-32583203 None:intergenic 80.0%
! GCGGCGGACGGCGACGGTGA+AGG - chr8_3:32583812-32583831 Msa1286000:intron 80.0%
! GCCCGGAGGGACGGCAGCGG+TGG + chr8_3:32583181-32583200 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 32581427 32584415 32581427 ID=Msa1286000;Name=Msa1286000
chr8_3 mRNA 32581427 32584415 32581427 ID=Msa1286000-mRNA-1;Parent=Msa1286000;Name=Msa1286000-mRNA-1;_AED=0.35;_eAED=0.47;_QI=46|0|0|1|0.33|0|4|0|153
chr8_3 exon 32584292 32584415 32584292 ID=Msa1286000-mRNA-1:exon:10055;Parent=Msa1286000-mRNA-1
chr8_3 exon 32582895 32583101 32582895 ID=Msa1286000-mRNA-1:exon:10054;Parent=Msa1286000-mRNA-1
chr8_3 exon 32581669 32581725 32581669 ID=Msa1286000-mRNA-1:exon:10053;Parent=Msa1286000-mRNA-1
chr8_3 exon 32581427 32581543 32581427 ID=Msa1286000-mRNA-1:exon:10052;Parent=Msa1286000-mRNA-1
chr8_3 five_prime_UTR 32584370 32584415 32584370 ID=Msa1286000-mRNA-1:five_prime_utr;Parent=Msa1286000-mRNA-1
chr8_3 CDS 32584292 32584369 32584292 ID=Msa1286000-mRNA-1:cds;Parent=Msa1286000-mRNA-1
chr8_3 CDS 32582895 32583101 32582895 ID=Msa1286000-mRNA-1:cds;Parent=Msa1286000-mRNA-1
chr8_3 CDS 32581669 32581725 32581669 ID=Msa1286000-mRNA-1:cds;Parent=Msa1286000-mRNA-1
chr8_3 CDS 32581427 32581543 32581427 ID=Msa1286000-mRNA-1:cds;Parent=Msa1286000-mRNA-1
Gene Sequence

>Msa1286000

ATGCCAGCAGATGCAATTGTAGACACCAAGGAAATCCAAGAAATGGAAGGGCCTTCATGGAAAGTGAGAAAAGAAGAGAGGGTCTTGTTCAAGATAATGGAAGTCCTTTTGAGATCAAAGGAAAGCTATTTCCCAAATGAAGTTCCTCCAATCACAGAAGGTACATCAGAAACAGTTGGTGCACTTTGGGACATATTTCGAAGAGAAGATACTGCAAAGTTAGAAGCATATCTTAGAAAATATTCCAAAGAATTTAGGCATACTTATTGTTCTCCAGTAAAAGAGCTTGGTGAAGCAGTATTTATACCTGCTGGATGCCCTCCCCAAGTTCGAAATCTGAAGTCATGCACAAAAGTTGCAGTAGACTTTGTGTCACCAGAAAATGTGGATATATGCATGCTTTTAACTGAAGAGTTTCGTCGCCTTCCTAAGAATCACAGGGCAAGAGAAGACAAACTT
Protein sequence

>Msa1286000

MPADAIVDTKEIQEMEGPSWKVRKEERVLFKIMEVLLRSKESYFPNEVPPITEGTSETVGALWDIFRREDTAKLEAYLRKYSKEFRHTYCSPVKELGEAVFIPAGCPPQVRNLKSCTKVAVDFVSPENVDICMLLTEEFRRLPKNHRAREDKL