AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1286580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1286580 MtrunA17_Chr8g0349251 96.078 102 4 0 1 102 1 102 4.98e-69 201
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1286580 AT5G44710.1 64.706 102 36 0 1 102 1 102 5.21e-47 146

Find 37 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCTTTCCTTGTCCCTTCTT+AGG 0.171222 8_3:-34756864 None:intergenic
AGAGTTGAAACCATCTAATT+TGG 0.216798 8_3:+34757064 Msa1286580:three_prime_UTR
AATTCATCCTATGTGTATAT+TGG 0.277518 8_3:+34756930 Msa1286580:three_prime_UTR
AAACTCTAAACGTAAGTTAA+TGG 0.300253 8_3:+34757224 Msa1286580:three_prime_UTR
GGTCATCAATTGAATCCTTC+AGG 0.310963 8_3:+34754648 Msa1286580:CDS
ATTTCTTATTGACCTCTTCC+TGG 0.339458 8_3:+34757021 Msa1286580:three_prime_UTR
GCTTTCCTTGTCCCTTCTTA+GGG 0.339596 8_3:-34756863 None:intergenic
CGTAAGTTAATGGTGTGTTT+TGG 0.368519 8_3:+34757234 Msa1286580:three_prime_UTR
ATTCTCCGTATGAAAATGTT+TGG 0.383146 8_3:+34754690 Msa1286580:CDS
CTAGAATAACAAAATCAAAA+AGG 0.385198 8_3:-34757173 None:intergenic
TCTGCACGAGCAACGATATT+CGG 0.397670 8_3:+34754627 Msa1286580:CDS
AGATCTCCAACCAAATTAGA+TGG 0.408977 8_3:-34757074 None:intergenic
TAAAGATGATCCCAATGTTA+TGG 0.410197 8_3:+34754749 Msa1286580:CDS
TTGAAACCATCTAATTTGGT+TGG 0.469958 8_3:+34757068 Msa1286580:three_prime_UTR
TGTTGACGAGCCATAACATT+GGG 0.470795 8_3:-34754759 None:intergenic
ATGTTGAAGCGTCGAGGTAA+AGG 0.476989 8_3:+34756837 Msa1286580:CDS
AACCTTGTGATAAACATTCA+AGG 0.499200 8_3:-34757139 None:intergenic
GTCATCAATTGAATCCTTCA+GGG 0.500225 8_3:+34754649 Msa1286580:CDS
TTCTTTCAGAACGAAGAAGA+TGG 0.512412 8_3:+34754551 Msa1286580:exon
TTTCTTATTGACCTCTTCCT+GGG 0.513599 8_3:+34757022 Msa1286580:three_prime_UTR
ATTTAAACCAATATACACAT+AGG 0.522661 8_3:-34756937 None:intergenic
TGTTATTTCTATTATCAAAG+AGG 0.528929 8_3:+34757274 Msa1286580:three_prime_UTR
TTTCCTTGTCCCTTCTTAGG+GGG 0.531658 8_3:-34756861 None:intergenic
GAAAGAAGATAATTGAATTG+TGG 0.539393 8_3:-34754535 None:intergenic
TCATCTTTATTGATATCATG+AGG 0.545670 8_3:-34754735 None:intergenic
TTCCTTGTCCCTTCTTAGGG+GGG 0.548132 8_3:-34756860 None:intergenic
TGAGCAGATTTCTTCCCTGA+AGG 0.553711 8_3:-34754663 None:intergenic
CTTTCCTTGTCCCTTCTTAG+GGG 0.574866 8_3:-34756862 None:intergenic
TACCTTGAATGTTTATCACA+AGG 0.599503 8_3:+34757137 Msa1286580:three_prime_UTR
ATGTTAACCTCTGCAATCAA+CGG 0.613390 8_3:+34754594 Msa1286580:CDS
CATCTTTATTGATATCATGA+GGG 0.630102 8_3:-34754734 None:intergenic
CTTGAAATGTTGAAGCGTCG+AGG 0.641392 8_3:+34756831 Msa1286580:CDS
TTGTTGACGAGCCATAACAT+TGG 0.647703 8_3:-34754760 None:intergenic
AATACCGCCGTTGATTGCAG+AGG 0.659011 8_3:-34754601 None:intergenic
AGAACGAAGAAGATGGCTAG+CGG 0.708452 8_3:+34754558 Msa1286580:exon
ACGAAGAAGATGGCTAGCGG+CGG 0.720581 8_3:+34754561 Msa1286580:exon
TTAACCTCTGCAATCAACGG+CGG 0.723215 8_3:+34754597 Msa1286580:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTAGTTTTTTTTTATGT+GGG + chr8_3:34755038-34755057 Msa1286580:intron 10.0%
!!! ATTGTTTTATTTTGATTAAT+GGG + chr8_3:34755061-34755080 Msa1286580:intron 10.0%
!!! TAAAGTTCAATTTTTTAAAT+TGG + chr8_3:34755097-34755116 Msa1286580:intron 10.0%
!!! AAAGTTCAATTTTTTAAATT+GGG + chr8_3:34755098-34755117 Msa1286580:intron 10.0%
!!! ATTTCAGTTTTTTTTTTAAG+TGG + chr8_3:34754928-34754947 Msa1286580:intron 15.0%
!!! TTTCAGTTTTTTTTTTAAGT+GGG + chr8_3:34754929-34754948 Msa1286580:intron 15.0%
!!! GTTTTTAGTTTTTTTTTATG+TGG + chr8_3:34755037-34755056 Msa1286580:intron 15.0%
!!! GATTGTTTTATTTTGATTAA+TGG + chr8_3:34755060-34755079 Msa1286580:intron 15.0%
!!! GATATTATTTTTTTTCAAAC+TGG - chr8_3:34756311-34756330 None:intergenic 15.0%
!!! GAAATTGTTTTCTTTTGTTT+TGG + chr8_3:34754850-34754869 Msa1286580:intron 20.0%
!! ATGCAGTTAATAGAAAAAAT+AGG + chr8_3:34755315-34755334 Msa1286580:intron 20.0%
!!! TTATTTTTTTTCAAACTGGA+GGG - chr8_3:34756307-34756326 None:intergenic 20.0%
!!! ATTATTTTTTTTCAAACTGG+AGG - chr8_3:34756308-34756327 None:intergenic 20.0%
!!! ATATTCTTAGTCTATTGTTT+TGG + chr8_3:34756444-34756463 Msa1286580:intron 20.0%
!! AATTAGTTAAAGTACGTAAA+TGG + chr8_3:34756586-34756605 Msa1286580:intron 20.0%
!!! ATATTTGGCATATTTTTTAC+TGG + chr8_3:34756731-34756750 Msa1286580:intron 20.0%
!! TATCAAAATTCTTGAATGAA+TGG - chr8_3:34756774-34756793 None:intergenic 20.0%
!! ATTTAAACCAATATACACAT+AGG - chr8_3:34756940-34756959 None:intergenic 20.0%
!! CTAGAATAACAAAATCAAAA+AGG - chr8_3:34757176-34757195 None:intergenic 20.0%
!!! TGTTATTTCTATTATCAAAG+AGG + chr8_3:34757274-34757293 Msa1286580:three_prime_UTR 20.0%
! GAAAGAAGATAATTGAATTG+TGG - chr8_3:34754538-34754557 None:intergenic 25.0%
!! TTTCACCAAACATTTTCATA+CGG - chr8_3:34754698-34754717 None:intergenic 25.0%
! CATCTTTATTGATATCATGA+GGG - chr8_3:34754737-34754756 None:intergenic 25.0%
! TCATCTTTATTGATATCATG+AGG - chr8_3:34754738-34754757 None:intergenic 25.0%
!!! TTTTGGAGTATTCTTATGTA+GGG + chr8_3:34754867-34754886 Msa1286580:intron 25.0%
!!! GCGTTATTTTCATTGATAAA+TGG + chr8_3:34754892-34754911 Msa1286580:intron 25.0%
!!! TTTTTTAAGTGGGTTTATGA+TGG + chr8_3:34754939-34754958 Msa1286580:intron 25.0%
!!! TTTTTAAGTGGGTTTATGAT+GGG + chr8_3:34754940-34754959 Msa1286580:intron 25.0%
!!! GTTTTATTTTGATTAATGGG+TGG + chr8_3:34755064-34755083 Msa1286580:intron 25.0%
! AATTGAGTTATGCAGTTAAT+AGG + chr8_3:34755145-34755164 Msa1286580:intron 25.0%
! TATGCAGTTAATAGGAAAAT+AGG + chr8_3:34755153-34755172 Msa1286580:intron 25.0%
!!! TATGAGAGTTGTTTTAATGA+AGG - chr8_3:34755257-34755276 None:intergenic 25.0%
!!! TTCGTTTTTCAGAAAAACAA+TGG + chr8_3:34755726-34755745 Msa1286580:intron 25.0%
!!! TCGTTTTTCAGAAAAACAAT+GGG + chr8_3:34755727-34755746 Msa1286580:intron 25.0%
! AGTACATACTTCAAAAACTT+TGG - chr8_3:34756695-34756714 None:intergenic 25.0%
!!! AGTTTTTGAAGTATGTACTT+TGG + chr8_3:34756696-34756715 Msa1286580:intron 25.0%
!!! GTTTTTGAAGTATGTACTTT+GGG + chr8_3:34756697-34756716 Msa1286580:intron 25.0%
! TGGGTTACAATCATAATATT+TGG + chr8_3:34756716-34756735 Msa1286580:intron 25.0%
! AATTCATCCTATGTGTATAT+TGG + chr8_3:34756930-34756949 Msa1286580:three_prime_UTR 25.0%
! AAACTCTAAACGTAAGTTAA+TGG + chr8_3:34757224-34757243 Msa1286580:three_prime_UTR 25.0%
ATTCTCCGTATGAAAATGTT+TGG + chr8_3:34754690-34754709 Msa1286580:CDS 30.0%
TTGGTGAAAAAGTTACTCAA+TGG + chr8_3:34754709-34754728 Msa1286580:CDS 30.0%
TAAAGATGATCCCAATGTTA+TGG + chr8_3:34754749-34754768 Msa1286580:CDS 30.0%
!!! GTTTTGGAGTATTCTTATGT+AGG + chr8_3:34754866-34754885 Msa1286580:intron 30.0%
!! TTTTAAGTGGGTTTATGATG+GGG + chr8_3:34754941-34754960 Msa1286580:intron 30.0%
ATGGTAATTCGAATCTATTG+AGG + chr8_3:34754965-34754984 Msa1286580:intron 30.0%
ATTGGGTATATTGTAGTGTT+TGG + chr8_3:34755115-34755134 Msa1286580:intron 30.0%
GCTCAAAAAACATGATATGA+GGG - chr8_3:34755272-34755291 None:intergenic 30.0%
TGCTCAAAAAACATGATATG+AGG - chr8_3:34755273-34755292 None:intergenic 30.0%
!!! ATGAATTGCTTTTTCCATCT+GGG + chr8_3:34755447-34755466 Msa1286580:intron 30.0%
TTTATTGAGTGAAATCCTTG+TGG + chr8_3:34755516-34755535 Msa1286580:intron 30.0%
TTATTGAGTGAAATCCTTGT+GGG + chr8_3:34755517-34755536 Msa1286580:intron 30.0%
TCTAACAGTCACTATCTTAT+CGG - chr8_3:34755600-34755619 None:intergenic 30.0%
ATTGCCCATGATATCATTTA+TGG + chr8_3:34755699-34755718 Msa1286580:intron 30.0%
TTGCCCATGATATCATTTAT+GGG + chr8_3:34755700-34755719 Msa1286580:intron 30.0%
TTCAGAAAAACAATGGGAAA+TGG + chr8_3:34755733-34755752 Msa1286580:intron 30.0%
TGAACTTATTTCCTCAATCA+AGG + chr8_3:34755836-34755855 Msa1286580:intron 30.0%
TGAACTGATCTCAACAATAA+TGG + chr8_3:34756005-34756024 Msa1286580:intron 30.0%
CTTAGATGCAATAAAAGAAG+TGG - chr8_3:34756037-34756056 None:intergenic 30.0%
! CACTTCTTTTATTGCATCTA+AGG + chr8_3:34756035-34756054 Msa1286580:intron 30.0%
CTTAGCTATAATTGAACTCA+AGG - chr8_3:34756061-34756080 None:intergenic 30.0%
CTTGAGTTCAATTATAGCTA+AGG + chr8_3:34756059-34756078 Msa1286580:intron 30.0%
TTATAGCTAAGGTCAATTAG+TGG + chr8_3:34756070-34756089 Msa1286580:intron 30.0%
TGAGTGAAGTGAAAAAATGT+AGG + chr8_3:34756145-34756164 Msa1286580:intron 30.0%
GAGTGAAGTGAAAAAATGTA+GGG + chr8_3:34756146-34756165 Msa1286580:intron 30.0%
AGGTGTTAACTACTTAGTAT+AGG + chr8_3:34756193-34756212 Msa1286580:intron 30.0%
TAACTACTTAGTATAGGAAG+AGG + chr8_3:34756199-34756218 Msa1286580:intron 30.0%
AACTGAAATTACCTCATTCA+TGG + chr8_3:34756421-34756440 Msa1286580:intron 30.0%
ACTAAGAATATCCATGAATG+AGG - chr8_3:34756435-34756454 None:intergenic 30.0%
!!! TTCTTAGTCTATTGTTTTGG+TGG + chr8_3:34756447-34756466 Msa1286580:intron 30.0%
! AAAGTTTTCTCTCTGAGTTT+TGG + chr8_3:34756478-34756497 Msa1286580:intron 30.0%
CAATGAACACATCTACAAAA+TGG - chr8_3:34756506-34756525 None:intergenic 30.0%
CCAATGTAGTCAAATTCATA+GGG - chr8_3:34756616-34756635 None:intergenic 30.0%
TACCATTAAAAGAATGCACT+TGG - chr8_3:34756645-34756664 None:intergenic 30.0%
AGAGTTGAAACCATCTAATT+TGG + chr8_3:34757064-34757083 Msa1286580:three_prime_UTR 30.0%
TTGAAACCATCTAATTTGGT+TGG + chr8_3:34757068-34757087 Msa1286580:three_prime_UTR 30.0%
TACCTTGAATGTTTATCACA+AGG + chr8_3:34757137-34757156 Msa1286580:three_prime_UTR 30.0%
AACCTTGTGATAAACATTCA+AGG - chr8_3:34757142-34757161 None:intergenic 30.0%
TTCTTTCAGAACGAAGAAGA+TGG + chr8_3:34754551-34754570 Msa1286580:exon 35.0%
ATGTTAACCTCTGCAATCAA+CGG + chr8_3:34754594-34754613 Msa1286580:CDS 35.0%
GTCATCAATTGAATCCTTCA+GGG + chr8_3:34754649-34754668 Msa1286580:CDS 35.0%
AAATCCCATGTGATGGTAAA+GGG + chr8_3:34755189-34755208 Msa1286580:intron 35.0%
AAACCGCATGTAATGGTAAA+GGG + chr8_3:34755351-34755370 Msa1286580:intron 35.0%
AATCCCTTTACCATTACATG+CGG - chr8_3:34755357-34755376 None:intergenic 35.0%
AGGGATTGAGTGAGTTTAAA+CGG + chr8_3:34755370-34755389 Msa1286580:intron 35.0%
!!! CATGAATTGCTTTTTCCATC+TGG + chr8_3:34755446-34755465 Msa1286580:intron 35.0%
!! AGAGTGAGTGTTGAAATTTG+TGG - chr8_3:34755496-34755515 None:intergenic 35.0%
TTTATGTGGGACCAATTTCT+AGG + chr8_3:34755549-34755568 Msa1286580:intron 35.0%
TTATGTGGGACCAATTTCTA+GGG + chr8_3:34755550-34755569 Msa1286580:intron 35.0%
CTAACAGTCACTATCTTATC+GGG - chr8_3:34755599-34755618 None:intergenic 35.0%
GAAAAGGTAATGTCAACAAG+AGG - chr8_3:34755653-34755672 None:intergenic 35.0%
ACAAAGCTCTCTTCAAGAAA+AGG - chr8_3:34755669-34755688 None:intergenic 35.0%
TGCCCATGATATCATTTATG+GGG + chr8_3:34755701-34755720 Msa1286580:intron 35.0%
AGCCCCATAAATGATATCAT+GGG - chr8_3:34755706-34755725 None:intergenic 35.0%
AAGCCCCATAAATGATATCA+TGG - chr8_3:34755707-34755726 None:intergenic 35.0%
GGTAGAATTTAGACCATCAA+TGG - chr8_3:34755772-34755791 None:intergenic 35.0%
GTCTAAATTCTACCCACATT+AGG + chr8_3:34755778-34755797 Msa1286580:intron 35.0%
TTCCTCAATCAAGGATTCAT+TGG + chr8_3:34755845-34755864 Msa1286580:intron 35.0%
AACCAATGAATCCTTGATTG+AGG - chr8_3:34755850-34755869 None:intergenic 35.0%
!! TCAAGGATTCATTGGTTTCA+TGG + chr8_3:34755853-34755872 Msa1286580:intron 35.0%
!! CAAGGATTCATTGGTTTCAT+GGG + chr8_3:34755854-34755873 Msa1286580:intron 35.0%
! CTATTGTTAGCTACTTTCCT+TGG + chr8_3:34755922-34755941 Msa1286580:intron 35.0%
! ATGTGAAGTGCTAGTTAAGA+TGG + chr8_3:34756119-34756138 Msa1286580:intron 35.0%
! TGTGAAGTGCTAGTTAAGAT+GGG + chr8_3:34756120-34756139 Msa1286580:intron 35.0%
TGAAGTGAAAAAATGTAGGG+AGG + chr8_3:34756149-34756168 Msa1286580:intron 35.0%
GAAGTGAAAAAATGTAGGGA+GGG + chr8_3:34756150-34756169 Msa1286580:intron 35.0%
!!! TCTATTGTTTTGGTGGTAGA+CGG + chr8_3:34756454-34756473 Msa1286580:intron 35.0%
AAGAGTCTAACACGTGAATA+AGG - chr8_3:34756536-34756555 None:intergenic 35.0%
CCCTATGAATTTGACTACAT+TGG + chr8_3:34756613-34756632 Msa1286580:intron 35.0%
GCCAATGTAGTCAAATTCAT+AGG - chr8_3:34756617-34756636 None:intergenic 35.0%
! CACCAAGTGCATTCTTTTAA+TGG + chr8_3:34756640-34756659 Msa1286580:intron 35.0%
ATTTCTTATTGACCTCTTCC+TGG + chr8_3:34757021-34757040 Msa1286580:three_prime_UTR 35.0%
TTTCTTATTGACCTCTTCCT+GGG + chr8_3:34757022-34757041 Msa1286580:three_prime_UTR 35.0%
AGATCTCCAACCAAATTAGA+TGG - chr8_3:34757077-34757096 None:intergenic 35.0%
! GTATCTTTTCGCTTACTGTT+TGG + chr8_3:34757201-34757220 Msa1286580:three_prime_UTR 35.0%
! CGTAAGTTAATGGTGTGTTT+TGG + chr8_3:34757234-34757253 Msa1286580:three_prime_UTR 35.0%
GGTCATCAATTGAATCCTTC+AGG + chr8_3:34754648-34754667 Msa1286580:CDS 40.0%
TGTTGACGAGCCATAACATT+GGG - chr8_3:34754762-34754781 None:intergenic 40.0%
TTGTTGACGAGCCATAACAT+TGG - chr8_3:34754763-34754782 None:intergenic 40.0%
TCTATTGAGGATTGCTCACT+AGG + chr8_3:34754978-34754997 Msa1286580:intron 40.0%
TGAACTGAAATCCCATGTGA+TGG + chr8_3:34755182-34755201 Msa1286580:intron 40.0%
GAAATCCCATGTGATGGTAA+AGG + chr8_3:34755188-34755207 Msa1286580:intron 40.0%
AATCCCATGTGATGGTAAAG+GGG + chr8_3:34755190-34755209 Msa1286580:intron 40.0%
TGAACTGAAACCGCATGTAA+TGG + chr8_3:34755344-34755363 Msa1286580:intron 40.0%
GAAACCGCATGTAATGGTAA+AGG + chr8_3:34755350-34755369 Msa1286580:intron 40.0%
GAAATTGGTCCCACATAAAG+TGG - chr8_3:34755548-34755567 None:intergenic 40.0%
ACTCCTAATGTTGCCATTGA+TGG + chr8_3:34755756-34755775 Msa1286580:intron 40.0%
AGACCATCAATGGCAACATT+AGG - chr8_3:34755762-34755781 None:intergenic 40.0%
CTGAACTTTCAGCCTAATGT+GGG - chr8_3:34755793-34755812 None:intergenic 40.0%
!! TCATTGGTTTCATGGGATGA+TGG + chr8_3:34755861-34755880 Msa1286580:intron 40.0%
ATGGAGAATACAAAACCAGC+TGG + chr8_3:34755880-34755899 Msa1286580:intron 40.0%
AAAACAACTCAGTAACCAGC+TGG - chr8_3:34755898-34755917 None:intergenic 40.0%
! GTTAGCTACTTTCCTTGGAT+CGG + chr8_3:34755927-34755946 Msa1286580:intron 40.0%
CTGATAAATGAACCGATCCA+AGG - chr8_3:34755942-34755961 None:intergenic 40.0%
AGCACTAAAATGATGCACGT+TGG - chr8_3:34755966-34755985 None:intergenic 40.0%
TTAGTGGCTGAGTTGTCAAA+TGG + chr8_3:34756086-34756105 Msa1286580:intron 40.0%
TAGTGGCTGAGTTGTCAAAT+GGG + chr8_3:34756087-34756106 Msa1286580:intron 40.0%
TGAAAAAATGTAGGGAGGGT+GGG + chr8_3:34756154-34756173 Msa1286580:intron 40.0%
CTACTTAGTATAGGAAGAGG+TGG + chr8_3:34756202-34756221 Msa1286580:intron 40.0%
CTGAGTGTGAAATTGAGAGA+GGG + chr8_3:34756230-34756249 Msa1286580:intron 40.0%
TGAGTGTGAAATTGAGAGAG+GGG + chr8_3:34756231-34756250 Msa1286580:intron 40.0%
ATCCACGTATGAGTACAGTA+GGG - chr8_3:34756374-34756393 None:intergenic 40.0%
TATCCACGTATGAGTACAGT+AGG - chr8_3:34756375-34756394 None:intergenic 40.0%
! AATGCATGAAGTTTTCTCCC+AGG - chr8_3:34757042-34757061 None:intergenic 40.0%
AGAACGAAGAAGATGGCTAG+CGG + chr8_3:34754558-34754577 Msa1286580:exon 45.0%
TTAACCTCTGCAATCAACGG+CGG + chr8_3:34754597-34754616 Msa1286580:CDS 45.0%
TCTGCACGAGCAACGATATT+CGG + chr8_3:34754627-34754646 Msa1286580:CDS 45.0%
TGAGCAGATTTCTTCCCTGA+AGG - chr8_3:34754666-34754685 None:intergenic 45.0%
! AGTGGGTTTATGATGGGGTA+TGG + chr8_3:34754946-34754965 Msa1286580:intron 45.0%
CAACCCCTTTACCATCACAT+GGG - chr8_3:34755196-34755215 None:intergenic 45.0%
TCAACCCCTTTACCATCACA+TGG - chr8_3:34755197-34755216 None:intergenic 45.0%
!!! TTGCTTTTTCCATCTGGGCA+CGG + chr8_3:34755452-34755471 Msa1286580:intron 45.0%
ATAAAGTGGTGTGACCCACA+AGG - chr8_3:34755534-34755553 None:intergenic 45.0%
TGGGTCACACCACTTTATGT+GGG + chr8_3:34755536-34755555 Msa1286580:intron 45.0%
TATCCCCACACCCTAGAAAT+TGG - chr8_3:34755563-34755582 None:intergenic 45.0%
!! ATGTCAACAAGAGGAGTGCT+AGG - chr8_3:34755644-34755663 None:intergenic 45.0%
GCTGAACTTTCAGCCTAATG+TGG - chr8_3:34755794-34755813 None:intergenic 45.0%
GCTTAGCAAGTGCTTTCTCT+AGG + chr8_3:34755812-34755831 Msa1286580:intron 45.0%
GTGAAAAAATGTAGGGAGGG+TGG + chr8_3:34756153-34756172 Msa1286580:intron 45.0%
GCTGAGTGTGAAATTGAGAG+AGG + chr8_3:34756229-34756248 Msa1286580:intron 45.0%
TGTGAAATTGAGAGAGGGGT+TGG + chr8_3:34756235-34756254 Msa1286580:intron 45.0%
! AAATTGAGAGAGGGGTTGGT+TGG + chr8_3:34756239-34756258 Msa1286580:intron 45.0%
!! AATTGAGAGAGGGGTTGGTT+GGG + chr8_3:34756240-34756259 Msa1286580:intron 45.0%
TACCCTACTGTACTCATACG+TGG + chr8_3:34756369-34756388 Msa1286580:intron 45.0%
!! CTTGAAATGTTGAAGCGTCG+AGG + chr8_3:34756831-34756850 Msa1286580:CDS 45.0%
!! ATGTTGAAGCGTCGAGGTAA+AGG + chr8_3:34756837-34756856 Msa1286580:CDS 45.0%
TTTCCTTGTCCCTTCTTAGG+GGG - chr8_3:34756864-34756883 None:intergenic 45.0%
CTTTCCTTGTCCCTTCTTAG+GGG - chr8_3:34756865-34756884 None:intergenic 45.0%
GCTTTCCTTGTCCCTTCTTA+GGG - chr8_3:34756866-34756885 None:intergenic 45.0%
! TGAAGTTTTCTCCCAGGAAG+AGG - chr8_3:34757036-34757055 None:intergenic 45.0%
! AATACCGCCGTTGATTGCAG+AGG - chr8_3:34754604-34754623 None:intergenic 50.0%
GTGGGTCACACCACTTTATG+TGG + chr8_3:34755535-34755554 Msa1286580:intron 50.0%
!! TGGGACCAATTTCTAGGGTG+TGG + chr8_3:34755555-34755574 Msa1286580:intron 50.0%
!! GGGACCAATTTCTAGGGTGT+GGG + chr8_3:34755556-34755575 Msa1286580:intron 50.0%
!! GGACCAATTTCTAGGGTGTG+GGG + chr8_3:34755557-34755576 Msa1286580:intron 50.0%
!! ATTGAGAGAGGGGTTGGTTG+GGG + chr8_3:34756241-34756260 Msa1286580:intron 50.0%
TTCCTTGTCCCTTCTTAGGG+GGG - chr8_3:34756863-34756882 None:intergenic 50.0%
CGCTTTCCTTGTCCCTTCTT+AGG - chr8_3:34756867-34756886 None:intergenic 50.0%
ACGAAGAAGATGGCTAGCGG+CGG + chr8_3:34754561-34754580 Msa1286580:exon 55.0%
TGGGAGCTAGCTAGCTAGCT+AGG + chr8_3:34756173-34756192 Msa1286580:intron 55.0%
! GTTGGGGAAGGTGTGGTGAA+GGG + chr8_3:34756257-34756276 Msa1286580:intron 55.0%
! TTGGGGAAGGTGTGGTGAAG+GGG + chr8_3:34756258-34756277 Msa1286580:intron 55.0%
AGGTAAAGGCCCCCCTAAGA+AGG + chr8_3:34756851-34756870 Msa1286580:CDS 55.0%
GGTAAAGGCCCCCCTAAGAA+GGG + chr8_3:34756852-34756871 Msa1286580:CDS 55.0%
GCAAAACGACCGTGCCCAGA+TGG - chr8_3:34755464-34755483 None:intergenic 60.0%
!! AGAGAGGGGTTGGTTGGGGA+AGG + chr8_3:34756245-34756264 Msa1286580:intron 60.0%
! GGTTGGGGAAGGTGTGGTGA+AGG + chr8_3:34756256-34756275 Msa1286580:intron 60.0%
! TGGGGAAGGTGTGGTGAAGG+GGG + chr8_3:34756259-34756278 Msa1286580:intron 60.0%
!! GGGGTTGGTTGGGGAAGGTG+TGG + chr8_3:34756250-34756269 Msa1286580:intron 65.0%
GGCCCCCCTAAGAAGGGACA+AGG + chr8_3:34756858-34756877 Msa1286580:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 34754528 34757316 34754528 ID=Msa1286580;Name=Msa1286580
chr8_3 mRNA 34754528 34757316 34754528 ID=Msa1286580-mRNA-1;Parent=Msa1286580;Name=Msa1286580-mRNA-1;_AED=0.00;_eAED=0.00;_QI=42|1|1|1|0|1|2|408|102
chr8_3 exon 34754528 34754787 34754528 ID=Msa1286580-mRNA-1:exon:10327;Parent=Msa1286580-mRNA-1
chr8_3 exon 34756818 34757316 34756818 ID=Msa1286580-mRNA-1:exon:10328;Parent=Msa1286580-mRNA-1
chr8_3 five_prime_UTR 34754528 34754569 34754528 ID=Msa1286580-mRNA-1:five_prime_utr;Parent=Msa1286580-mRNA-1
chr8_3 CDS 34754570 34754787 34754570 ID=Msa1286580-mRNA-1:cds;Parent=Msa1286580-mRNA-1
chr8_3 CDS 34756818 34756908 34756818 ID=Msa1286580-mRNA-1:cds;Parent=Msa1286580-mRNA-1
chr8_3 three_prime_UTR 34756909 34757316 34756909 ID=Msa1286580-mRNA-1:three_prime_utr;Parent=Msa1286580-mRNA-1
Gene Sequence

>Msa1286580

ATGGCTAGCGGCGGTTTGAAGAAGATGTTAACCTCTGCAATCAACGGCGGTATTACTTCTGCACGAGCAACGATATTCGGTCATCAATTGAATCCTTCAGGGAAGAAATCTGCTCACAAAATTCTCCGTATGAAAATGTTTGGTGAAAAAGTTACTCAATGGTACCCTCATGATATCAATAAAGATGATCCCAATGTTATGGCTCGTCAACAACAAGTACGTTTATCGAAGCTTGAAATGTTGAAGCGTCGAGGTAAAGGCCCCCCTAAGAAGGGACAAGGAAAGCGTGCTAAAAAAAGCAACAAATAG
Protein sequence

>Msa1286580

MASGGLKKMLTSAINGGITSARATIFGHQLNPSGKKSAHKILRMKMFGEKVTQWYPHDINKDDPNVMARQQQVRLSKLEMLKRRGKGPPKKGQGKRAKKSNK*