AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0840180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 30 sgRNAs with CRISPR-Local

Find 258 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATCTTCTGTTCATAAAAT+TGG 0.134160 5_4:+15760731 Msa0840180:CDS
AGGTCTCGTCACATTGAATT+TGG 0.174216 5_4:+15760701 Msa0840180:CDS
AAGCACAAGGTGTGCCTAAA+AGG 0.210103 5_4:-15762676 None:intergenic
GCCAACATCAAATCAACTTA+TGG 0.253094 5_4:+15760619 Msa0840180:exon
TGTGTAACACAACGAGCTTC+AGG 0.310182 5_4:+15762521 Msa0840180:three_prime_UTR
ACTTTCTGTTGAGTCTCGAC+TGG 0.373300 5_4:-15762424 None:intergenic
AGCAAGTAGAGTAAACATTA+TGG 0.375216 5_4:-15762652 None:intergenic
GGTCTCGTCACATTGAATTT+GGG 0.382854 5_4:+15760702 Msa0840180:CDS
CCGGTGGCGATCCAGTAAAA+AGG 0.455562 5_4:+15760670 Msa0840180:CDS
CTTTCTGTTGAGTCTCGACT+GGG 0.456306 5_4:-15762423 None:intergenic
TCCATAAGTTGATTTGATGT+TGG 0.459028 5_4:-15760620 None:intergenic
AGGCACACCTTGTGCTTCAA+AGG 0.465103 5_4:+15762682 Msa0840180:three_prime_UTR
GACGAAGAATAAAATATGTA+AGG 0.477766 5_4:+15762786 Msa0840180:three_prime_UTR
TCCAGAGCTATATTGGGAGT+CGG 0.486378 5_4:+15762367 Msa0840180:intron
GGCACACCTTGTGCTTCAAA+GGG 0.487026 5_4:+15762683 Msa0840180:three_prime_UTR
ACGAGTAGCGGAGGAGAAAG+TGG 0.489711 5_4:+15762559 Msa0840180:three_prime_UTR
CCAGAGCTATATTGGGAGTC+GGG 0.499074 5_4:+15762368 Msa0840180:intron
AATGCGCCGACAAATATTAC+CGG 0.508412 5_4:+15760651 Msa0840180:CDS
AGCACAAGGTGTGCCTAAAA+GGG 0.516690 5_4:-15762675 None:intergenic
GTGTAACACAACGAGCTTCA+GGG 0.521715 5_4:+15762522 Msa0840180:three_prime_UTR
AAATCAAGACTTAAATTCGA+TGG 0.526399 5_4:+15762753 Msa0840180:three_prime_UTR
TCGCCACCGGTAATATTTGT+CGG 0.542553 5_4:-15760657 None:intergenic
CTGAGATGGGCTGAGAGAAG+AGG 0.580583 5_4:-15760592 None:intergenic
TGATGTTGGCGAAACTGAGA+TGG 0.581790 5_4:-15760606 None:intergenic
GATGTTGGCGAAACTGAGAT+GGG 0.609774 5_4:-15760605 None:intergenic
AAAACTCCCTTTGAAGCACA+AGG 0.615275 5_4:-15762689 None:intergenic
GAGCTATATTGGGAGTCGGG+TGG 0.629738 5_4:+15762371 Msa0840180:intron
CTTTGTCATAAAACGAGTAG+CGG 0.644655 5_4:+15762547 Msa0840180:three_prime_UTR
TGTCATAAAACGAGTAGCGG+AGG 0.659127 5_4:+15762550 Msa0840180:three_prime_UTR
GCGCCGACAAATATTACCGG+TGG 0.694721 5_4:+15760654 Msa0840180:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTCTTTTACTTTTTTTT+AGG + chr5_4:15762612-15762631 Msa0840180:three_prime_UTR 10.0%
!!! TTTTTAATCTATTTTGTTTT+TGG + chr5_4:15762709-15762728 Msa0840180:three_prime_UTR 10.0%
!!! ATTTTCTTTTACTTTTTTTT+AGG + chr5_4:15762612-15762631 Msa0840180:three_prime_UTR 10.0%
!!! TTTTTAATCTATTTTGTTTT+TGG + chr5_4:15762709-15762728 Msa0840180:three_prime_UTR 10.0%
!!! AATGTTATCATTGATTTTTT+TGG + chr5_4:15760902-15760921 Msa0840180:intron 15.0%
!!! GTTTGTGTTTTTTTTTTTTA+AGG + chr5_4:15760942-15760961 Msa0840180:intron 15.0%
!! ACAATCATTAATCATAAAAA+GGG - chr5_4:15761099-15761118 None:intergenic 15.0%
!! AACAATCATTAATCATAAAA+AGG - chr5_4:15761100-15761119 None:intergenic 15.0%
!!! AATGTTATCATTGATTTTTT+TGG + chr5_4:15760902-15760921 Msa0840180:intron 15.0%
!!! GTTTGTGTTTTTTTTTTTTA+AGG + chr5_4:15760942-15760961 Msa0840180:intron 15.0%
!! ACAATCATTAATCATAAAAA+GGG - chr5_4:15761099-15761118 None:intergenic 15.0%
!! AACAATCATTAATCATAAAA+AGG - chr5_4:15761100-15761119 None:intergenic 15.0%
!!! TTTTCTCATTGTTACTTATT+TGG + chr5_4:15760780-15760799 Msa0840180:intron 20.0%
!!! TTTCTCATTGTTACTTATTT+GGG + chr5_4:15760781-15760800 Msa0840180:intron 20.0%
!! CAATCATTAATCATAAAAAG+GGG - chr5_4:15761098-15761117 None:intergenic 20.0%
!! TACAATTAGGTAAAACTATT+TGG - chr5_4:15761179-15761198 None:intergenic 20.0%
!! AAATCATGAACAACAAAAAT+AGG - chr5_4:15761864-15761883 None:intergenic 20.0%
!! ACAATAAATTACAGTTAATG+TGG - chr5_4:15761893-15761912 None:intergenic 20.0%
!! AACTTATTCACATTTCAAAT+TGG - chr5_4:15762092-15762111 None:intergenic 20.0%
!!! TGCAGATGTTAAAAATATTT+TGG + chr5_4:15762320-15762339 Msa0840180:intron 20.0%
!!! TTACATATTTTATTCTTCGT+CGG - chr5_4:15762787-15762806 None:intergenic 20.0%
!!! TTTTCTCATTGTTACTTATT+TGG + chr5_4:15760780-15760799 Msa0840180:intron 20.0%
!!! TTTCTCATTGTTACTTATTT+GGG + chr5_4:15760781-15760800 Msa0840180:intron 20.0%
!! CAATCATTAATCATAAAAAG+GGG - chr5_4:15761098-15761117 None:intergenic 20.0%
!! TACAATTAGGTAAAACTATT+TGG - chr5_4:15761179-15761198 None:intergenic 20.0%
!! AAATCATGAACAACAAAAAT+AGG - chr5_4:15761864-15761883 None:intergenic 20.0%
!! ACAATAAATTACAGTTAATG+TGG - chr5_4:15761893-15761912 None:intergenic 20.0%
!! AACTTATTCACATTTCAAAT+TGG - chr5_4:15762092-15762111 None:intergenic 20.0%
!!! TGCAGATGTTAAAAATATTT+TGG + chr5_4:15762320-15762339 Msa0840180:intron 20.0%
!!! TTACATATTTTATTCTTCGT+CGG - chr5_4:15762787-15762806 None:intergenic 20.0%
! AGATCTTCTGTTCATAAAAT+TGG + chr5_4:15760731-15760750 Msa0840180:CDS 25.0%
!!! TTTGGTTTGTTTTGTGTTTA+AGG + chr5_4:15760920-15760939 Msa0840180:intron 25.0%
! TCACATATCACATAATATCT+AGG - chr5_4:15761124-15761143 None:intergenic 25.0%
!! AAACTATTTGGTTTACAAGA+AGG - chr5_4:15761167-15761186 None:intergenic 25.0%
!! GCAATTTTGTCATTACAATT+AGG - chr5_4:15761192-15761211 None:intergenic 25.0%
! ATTGTAATGACAAAATTGCT+AGG + chr5_4:15761193-15761212 Msa0840180:intron 25.0%
! TTGTAATGACAAAATTGCTA+GGG + chr5_4:15761194-15761213 Msa0840180:intron 25.0%
!!! AAGCAGAGTTTTATCTAATT+AGG + chr5_4:15761244-15761263 Msa0840180:intron 25.0%
!!! AGCAGAGTTTTATCTAATTA+GGG + chr5_4:15761245-15761264 Msa0840180:intron 25.0%
! TAACTAAGGACAAAATTACT+AGG + chr5_4:15761321-15761340 Msa0840180:intron 25.0%
! AACTAAGGACAAAATTACTA+GGG + chr5_4:15761322-15761341 Msa0840180:intron 25.0%
! TATAGTCAGGTAAATTATGA+GGG - chr5_4:15761554-15761573 None:intergenic 25.0%
! ATATAGTCAGGTAAATTATG+AGG - chr5_4:15761555-15761574 None:intergenic 25.0%
! TTGTCATGTAAAATATAGTC+AGG - chr5_4:15761567-15761586 None:intergenic 25.0%
! TATAGTCAGGTAAATTATGA+GGG - chr5_4:15761554-15761573 None:intergenic 25.0%
! ATATAGTCAGGTAAATTATG+AGG - chr5_4:15761555-15761574 None:intergenic 25.0%
! TTGTCATGTAAAATATAGTC+AGG - chr5_4:15761567-15761586 None:intergenic 25.0%
! ACTATATAGAAGTTAAGCAT+AGG - chr5_4:15761777-15761796 None:intergenic 25.0%
! TATGCTTAACTTCTATATAG+TGG + chr5_4:15761776-15761795 Msa0840180:intron 25.0%
!! GAGCTTAATCTCATATTTTA+AGG + chr5_4:15761993-15762012 Msa0840180:intron 25.0%
! AAATTTCTGCTCAAGAAATA+TGG - chr5_4:15762028-15762047 None:intergenic 25.0%
!! TATTTCTTGAGCAGAAATTT+TGG + chr5_4:15762028-15762047 Msa0840180:intron 25.0%
!! TTGGTAATTAGCACAAATAT+GGG - chr5_4:15762073-15762092 None:intergenic 25.0%
!! ATTGGTAATTAGCACAAATA+TGG - chr5_4:15762074-15762093 None:intergenic 25.0%
! ATCATCATTAATCTGTATCT+AGG + chr5_4:15762233-15762252 Msa0840180:intron 25.0%
!!! ATATTTTGGTGATAAACTGA+TGG + chr5_4:15762334-15762353 Msa0840180:intron 25.0%
! AAATCAAGACTTAAATTCGA+TGG + chr5_4:15762753-15762772 Msa0840180:three_prime_UTR 25.0%
! AGATCTTCTGTTCATAAAAT+TGG + chr5_4:15760731-15760750 Msa0840180:CDS 25.0%
!!! TTTGGTTTGTTTTGTGTTTA+AGG + chr5_4:15760920-15760939 Msa0840180:intron 25.0%
! TCACATATCACATAATATCT+AGG - chr5_4:15761124-15761143 None:intergenic 25.0%
!! AAACTATTTGGTTTACAAGA+AGG - chr5_4:15761167-15761186 None:intergenic 25.0%
!! GCAATTTTGTCATTACAATT+AGG - chr5_4:15761192-15761211 None:intergenic 25.0%
! ATTGTAATGACAAAATTGCT+AGG + chr5_4:15761193-15761212 Msa0840180:intron 25.0%
! TTGTAATGACAAAATTGCTA+GGG + chr5_4:15761194-15761213 Msa0840180:intron 25.0%
!!! AAGCAGAGTTTTATCTAATT+AGG + chr5_4:15761244-15761263 Msa0840180:intron 25.0%
!!! AGCAGAGTTTTATCTAATTA+GGG + chr5_4:15761245-15761264 Msa0840180:intron 25.0%
! TAACTAAGGACAAAATTACT+AGG + chr5_4:15761321-15761340 Msa0840180:intron 25.0%
! AACTAAGGACAAAATTACTA+GGG + chr5_4:15761322-15761341 Msa0840180:intron 25.0%
! TATAGTCAGGTAAATTATGA+GGG - chr5_4:15761554-15761573 None:intergenic 25.0%
! ATATAGTCAGGTAAATTATG+AGG - chr5_4:15761555-15761574 None:intergenic 25.0%
! TTGTCATGTAAAATATAGTC+AGG - chr5_4:15761567-15761586 None:intergenic 25.0%
! TATAGTCAGGTAAATTATGA+GGG - chr5_4:15761554-15761573 None:intergenic 25.0%
! ATATAGTCAGGTAAATTATG+AGG - chr5_4:15761555-15761574 None:intergenic 25.0%
! TTGTCATGTAAAATATAGTC+AGG - chr5_4:15761567-15761586 None:intergenic 25.0%
! ACTATATAGAAGTTAAGCAT+AGG - chr5_4:15761777-15761796 None:intergenic 25.0%
! TATGCTTAACTTCTATATAG+TGG + chr5_4:15761776-15761795 Msa0840180:intron 25.0%
!! GAGCTTAATCTCATATTTTA+AGG + chr5_4:15761993-15762012 Msa0840180:intron 25.0%
! AAATTTCTGCTCAAGAAATA+TGG - chr5_4:15762028-15762047 None:intergenic 25.0%
!! TATTTCTTGAGCAGAAATTT+TGG + chr5_4:15762028-15762047 Msa0840180:intron 25.0%
!! TTGGTAATTAGCACAAATAT+GGG - chr5_4:15762073-15762092 None:intergenic 25.0%
!! ATTGGTAATTAGCACAAATA+TGG - chr5_4:15762074-15762093 None:intergenic 25.0%
! ATCATCATTAATCTGTATCT+AGG + chr5_4:15762233-15762252 Msa0840180:intron 25.0%
!!! ATATTTTGGTGATAAACTGA+TGG + chr5_4:15762334-15762353 Msa0840180:intron 25.0%
! AAATCAAGACTTAAATTCGA+TGG + chr5_4:15762753-15762772 Msa0840180:three_prime_UTR 25.0%
!! TCCATAAGTTGATTTGATGT+TGG - chr5_4:15760623-15760642 None:intergenic 30.0%
! CCTATTATACTCCTTTTTAC+TGG - chr5_4:15760684-15760703 None:intergenic 30.0%
! CCAGTAAAAAGGAGTATAAT+AGG + chr5_4:15760681-15760700 Msa0840180:CDS 30.0%
!! TGTTTGTGTTGGTTCTTTAT+AGG - chr5_4:15760825-15760844 None:intergenic 30.0%
TTGGTCAAATATCTGAATTG+AGG + chr5_4:15761032-15761051 Msa0840180:intron 30.0%
! CTATATAGTGGAAGTTACTA+TGG + chr5_4:15761788-15761807 Msa0840180:intron 30.0%
TGTGAATAAGTTTGTAGCAA+TGG + chr5_4:15762100-15762119 Msa0840180:intron 30.0%
ATAGTTATCTATCTGTTGAG+TGG + chr5_4:15762169-15762188 Msa0840180:intron 30.0%
ATCATTAATCTGTATCTAGG+CGG + chr5_4:15762236-15762255 Msa0840180:intron 30.0%
! ATTTTTCTCCAGAGCTATAT+TGG + chr5_4:15762360-15762379 Msa0840180:intron 30.0%
! TTTTTCTCCAGAGCTATATT+GGG + chr5_4:15762361-15762380 Msa0840180:intron 30.0%
!! CTTTTTAGAGAAAACACTGA+AGG + chr5_4:15762451-15762470 Msa0840180:CDS 30.0%
AGCAAGTAGAGTAAACATTA+TGG - chr5_4:15762655-15762674 None:intergenic 30.0%
!! TCCATAAGTTGATTTGATGT+TGG - chr5_4:15760623-15760642 None:intergenic 30.0%
! CCTATTATACTCCTTTTTAC+TGG - chr5_4:15760684-15760703 None:intergenic 30.0%
! CCAGTAAAAAGGAGTATAAT+AGG + chr5_4:15760681-15760700 Msa0840180:CDS 30.0%
!! TGTTTGTGTTGGTTCTTTAT+AGG - chr5_4:15760825-15760844 None:intergenic 30.0%
TTGGTCAAATATCTGAATTG+AGG + chr5_4:15761032-15761051 Msa0840180:intron 30.0%
! CTATATAGTGGAAGTTACTA+TGG + chr5_4:15761788-15761807 Msa0840180:intron 30.0%
TGTGAATAAGTTTGTAGCAA+TGG + chr5_4:15762100-15762119 Msa0840180:intron 30.0%
ATAGTTATCTATCTGTTGAG+TGG + chr5_4:15762169-15762188 Msa0840180:intron 30.0%
ATCATTAATCTGTATCTAGG+CGG + chr5_4:15762236-15762255 Msa0840180:intron 30.0%
! ATTTTTCTCCAGAGCTATAT+TGG + chr5_4:15762360-15762379 Msa0840180:intron 30.0%
! TTTTTCTCCAGAGCTATATT+GGG + chr5_4:15762361-15762380 Msa0840180:intron 30.0%
!! CTTTTTAGAGAAAACACTGA+AGG + chr5_4:15762451-15762470 Msa0840180:CDS 30.0%
AGCAAGTAGAGTAAACATTA+TGG - chr5_4:15762655-15762674 None:intergenic 30.0%
GCCAACATCAAATCAACTTA+TGG + chr5_4:15760619-15760638 Msa0840180:exon 35.0%
GCTACAGTGATATGTAGATA+CGG + chr5_4:15760851-15760870 Msa0840180:intron 35.0%
GAGAAAAGATGCTGAATTGT+TGG + chr5_4:15761013-15761032 Msa0840180:intron 35.0%
! AATTGCTAGGGTTTGAAGTT+AGG + chr5_4:15761206-15761225 Msa0840180:intron 35.0%
ACGAGTGTTTATAAACTACC+CGG - chr5_4:15761277-15761296 None:intergenic 35.0%
TAATTCTTCAGTCGAGGTTA+AGG - chr5_4:15761430-15761449 None:intergenic 35.0%
ATCAAGTAATTCTTCAGTCG+AGG - chr5_4:15761436-15761455 None:intergenic 35.0%
TAATTCTTCAGTCGAGGTTA+AGG - chr5_4:15761430-15761449 None:intergenic 35.0%
ATCAAGTAATTCTTCAGTCG+AGG - chr5_4:15761436-15761455 None:intergenic 35.0%
TGTTAAGTATGAGAGCCATT+TGG + chr5_4:15761912-15761931 Msa0840180:intron 35.0%
TGCAGATTCTTTAAGTCTGT+AGG + chr5_4:15761945-15761964 Msa0840180:intron 35.0%
TATGGGAATCTTACTCTGAT+AGG - chr5_4:15762056-15762075 None:intergenic 35.0%
TCATTAATCTGTATCTAGGC+GGG + chr5_4:15762237-15762256 Msa0840180:intron 35.0%
CTTTGTCATAAAACGAGTAG+CGG + chr5_4:15762547-15762566 Msa0840180:three_prime_UTR 35.0%
GCCAACATCAAATCAACTTA+TGG + chr5_4:15760619-15760638 Msa0840180:exon 35.0%
GCTACAGTGATATGTAGATA+CGG + chr5_4:15760851-15760870 Msa0840180:intron 35.0%
GAGAAAAGATGCTGAATTGT+TGG + chr5_4:15761013-15761032 Msa0840180:intron 35.0%
! AATTGCTAGGGTTTGAAGTT+AGG + chr5_4:15761206-15761225 Msa0840180:intron 35.0%
ACGAGTGTTTATAAACTACC+CGG - chr5_4:15761277-15761296 None:intergenic 35.0%
TAATTCTTCAGTCGAGGTTA+AGG - chr5_4:15761430-15761449 None:intergenic 35.0%
ATCAAGTAATTCTTCAGTCG+AGG - chr5_4:15761436-15761455 None:intergenic 35.0%
TAATTCTTCAGTCGAGGTTA+AGG - chr5_4:15761430-15761449 None:intergenic 35.0%
ATCAAGTAATTCTTCAGTCG+AGG - chr5_4:15761436-15761455 None:intergenic 35.0%
TGTTAAGTATGAGAGCCATT+TGG + chr5_4:15761912-15761931 Msa0840180:intron 35.0%
TGCAGATTCTTTAAGTCTGT+AGG + chr5_4:15761945-15761964 Msa0840180:intron 35.0%
TATGGGAATCTTACTCTGAT+AGG - chr5_4:15762056-15762075 None:intergenic 35.0%
TCATTAATCTGTATCTAGGC+GGG + chr5_4:15762237-15762256 Msa0840180:intron 35.0%
CTTTGTCATAAAACGAGTAG+CGG + chr5_4:15762547-15762566 Msa0840180:three_prime_UTR 35.0%
! AATGCGCCGACAAATATTAC+CGG + chr5_4:15760651-15760670 Msa0840180:CDS 40.0%
AGGTCTCGTCACATTGAATT+TGG + chr5_4:15760701-15760720 Msa0840180:CDS 40.0%
GGTCTCGTCACATTGAATTT+GGG + chr5_4:15760702-15760721 Msa0840180:CDS 40.0%
GTAGCTATCGTTGTTTGTGT+TGG - chr5_4:15760836-15760855 None:intergenic 40.0%
TATCTAATTAGGGTCAGAGC+CGG + chr5_4:15761255-15761274 Msa0840180:intron 40.0%
GAGTCTGACTCTTCTAACTA+AGG + chr5_4:15761307-15761326 Msa0840180:intron 40.0%
TCTACATCAATGTTCCAACC+AGG - chr5_4:15761359-15761378 None:intergenic 40.0%
GGTTGGAACATTGATGTAGA+AGG + chr5_4:15761359-15761378 Msa0840180:intron 40.0%
GGTAAATTATGAGGGAAGTG+AGG - chr5_4:15761546-15761565 None:intergenic 40.0%
GGTAAATTATGAGGGAAGTG+AGG - chr5_4:15761546-15761565 None:intergenic 40.0%
ATAAGTTTGTAGCAATGGCC+TGG + chr5_4:15762105-15762124 Msa0840180:intron 40.0%
GATCCAATTCATCATAAGCC+AGG - chr5_4:15762126-15762145 None:intergenic 40.0%
GATAGATAACTATGATGCGC+AGG - chr5_4:15762162-15762181 None:intergenic 40.0%
! TACTCTACTTGCTCCCTTTT+AGG + chr5_4:15762662-15762681 Msa0840180:three_prime_UTR 40.0%
!! AAAACTCCCTTTGAAGCACA+AGG - chr5_4:15762692-15762711 None:intergenic 40.0%
! AATGCGCCGACAAATATTAC+CGG + chr5_4:15760651-15760670 Msa0840180:CDS 40.0%
AGGTCTCGTCACATTGAATT+TGG + chr5_4:15760701-15760720 Msa0840180:CDS 40.0%
GGTCTCGTCACATTGAATTT+GGG + chr5_4:15760702-15760721 Msa0840180:CDS 40.0%
GTAGCTATCGTTGTTTGTGT+TGG - chr5_4:15760836-15760855 None:intergenic 40.0%
TATCTAATTAGGGTCAGAGC+CGG + chr5_4:15761255-15761274 Msa0840180:intron 40.0%
GAGTCTGACTCTTCTAACTA+AGG + chr5_4:15761307-15761326 Msa0840180:intron 40.0%
TCTACATCAATGTTCCAACC+AGG - chr5_4:15761359-15761378 None:intergenic 40.0%
GGTTGGAACATTGATGTAGA+AGG + chr5_4:15761359-15761378 Msa0840180:intron 40.0%
GGTAAATTATGAGGGAAGTG+AGG - chr5_4:15761546-15761565 None:intergenic 40.0%
GGTAAATTATGAGGGAAGTG+AGG - chr5_4:15761546-15761565 None:intergenic 40.0%
ATAAGTTTGTAGCAATGGCC+TGG + chr5_4:15762105-15762124 Msa0840180:intron 40.0%
GATCCAATTCATCATAAGCC+AGG - chr5_4:15762126-15762145 None:intergenic 40.0%
GATAGATAACTATGATGCGC+AGG - chr5_4:15762162-15762181 None:intergenic 40.0%
! TACTCTACTTGCTCCCTTTT+AGG + chr5_4:15762662-15762681 Msa0840180:three_prime_UTR 40.0%
!! AAAACTCCCTTTGAAGCACA+AGG - chr5_4:15762692-15762711 None:intergenic 40.0%
GATGTTGGCGAAACTGAGAT+GGG - chr5_4:15760608-15760627 None:intergenic 45.0%
TGATGTTGGCGAAACTGAGA+TGG - chr5_4:15760609-15760628 None:intergenic 45.0%
TCGCCACCGGTAATATTTGT+CGG - chr5_4:15760660-15760679 None:intergenic 45.0%
! GGTTTACAAGAAGGTGCATC+TGG - chr5_4:15761158-15761177 None:intergenic 45.0%
ATCTAATTAGGGTCAGAGCC+GGG + chr5_4:15761256-15761275 Msa0840180:intron 45.0%
ACTAGGGTTCACTTAAGACC+TGG + chr5_4:15761338-15761357 Msa0840180:intron 45.0%
TTGATGTAGAAGGAGAGCCT+AGG + chr5_4:15761369-15761388 Msa0840180:intron 45.0%
GTCGAGGTTAAGGAGCTAAA+GGG - chr5_4:15761420-15761439 None:intergenic 45.0%
AGTCGAGGTTAAGGAGCTAA+AGG - chr5_4:15761421-15761440 None:intergenic 45.0%
AGCTAAAGGGTTGTCAGCAT+AGG - chr5_4:15761592-15761611 None:intergenic 45.0%
GTCGAGGTTAAGGAGCTAAA+GGG - chr5_4:15761420-15761439 None:intergenic 45.0%
AGTCGAGGTTAAGGAGCTAA+AGG - chr5_4:15761421-15761440 None:intergenic 45.0%
TGGCCTGGCTTATGATGAAT+TGG + chr5_4:15762120-15762139 Msa0840180:intron 45.0%
TATGATGCGCAGGTTACTGT+GGG - chr5_4:15762152-15762171 None:intergenic 45.0%
ATCTAGGCGGGATAAACTGA+TGG + chr5_4:15762249-15762268 Msa0840180:intron 45.0%
TCCAGAGCTATATTGGGAGT+CGG + chr5_4:15762367-15762386 Msa0840180:intron 45.0%
CTTTCTGTTGAGTCTCGACT+GGG - chr5_4:15762426-15762445 None:intergenic 45.0%
ACTTTCTGTTGAGTCTCGAC+TGG - chr5_4:15762427-15762446 None:intergenic 45.0%
TGTGTAACACAACGAGCTTC+AGG + chr5_4:15762521-15762540 Msa0840180:three_prime_UTR 45.0%
GTGTAACACAACGAGCTTCA+GGG + chr5_4:15762522-15762541 Msa0840180:three_prime_UTR 45.0%
TGTCATAAAACGAGTAGCGG+AGG + chr5_4:15762550-15762569 Msa0840180:three_prime_UTR 45.0%
AGCACAAGGTGTGCCTAAAA+GGG - chr5_4:15762678-15762697 None:intergenic 45.0%
AAGCACAAGGTGTGCCTAAA+AGG - chr5_4:15762679-15762698 None:intergenic 45.0%
GATGTTGGCGAAACTGAGAT+GGG - chr5_4:15760608-15760627 None:intergenic 45.0%
TGATGTTGGCGAAACTGAGA+TGG - chr5_4:15760609-15760628 None:intergenic 45.0%
TCGCCACCGGTAATATTTGT+CGG - chr5_4:15760660-15760679 None:intergenic 45.0%
! GGTTTACAAGAAGGTGCATC+TGG - chr5_4:15761158-15761177 None:intergenic 45.0%
ATCTAATTAGGGTCAGAGCC+GGG + chr5_4:15761256-15761275 Msa0840180:intron 45.0%
ACTAGGGTTCACTTAAGACC+TGG + chr5_4:15761338-15761357 Msa0840180:intron 45.0%
TTGATGTAGAAGGAGAGCCT+AGG + chr5_4:15761369-15761388 Msa0840180:intron 45.0%
GTCGAGGTTAAGGAGCTAAA+GGG - chr5_4:15761420-15761439 None:intergenic 45.0%
AGTCGAGGTTAAGGAGCTAA+AGG - chr5_4:15761421-15761440 None:intergenic 45.0%
AGCTAAAGGGTTGTCAGCAT+AGG - chr5_4:15761592-15761611 None:intergenic 45.0%
GTCGAGGTTAAGGAGCTAAA+GGG - chr5_4:15761420-15761439 None:intergenic 45.0%
AGTCGAGGTTAAGGAGCTAA+AGG - chr5_4:15761421-15761440 None:intergenic 45.0%
TGGCCTGGCTTATGATGAAT+TGG + chr5_4:15762120-15762139 Msa0840180:intron 45.0%
TATGATGCGCAGGTTACTGT+GGG - chr5_4:15762152-15762171 None:intergenic 45.0%
ATCTAGGCGGGATAAACTGA+TGG + chr5_4:15762249-15762268 Msa0840180:intron 45.0%
TCCAGAGCTATATTGGGAGT+CGG + chr5_4:15762367-15762386 Msa0840180:intron 45.0%
CTTTCTGTTGAGTCTCGACT+GGG - chr5_4:15762426-15762445 None:intergenic 45.0%
ACTTTCTGTTGAGTCTCGAC+TGG - chr5_4:15762427-15762446 None:intergenic 45.0%
TGTGTAACACAACGAGCTTC+AGG + chr5_4:15762521-15762540 Msa0840180:three_prime_UTR 45.0%
GTGTAACACAACGAGCTTCA+GGG + chr5_4:15762522-15762541 Msa0840180:three_prime_UTR 45.0%
TGTCATAAAACGAGTAGCGG+AGG + chr5_4:15762550-15762569 Msa0840180:three_prime_UTR 45.0%
AGCACAAGGTGTGCCTAAAA+GGG - chr5_4:15762678-15762697 None:intergenic 45.0%
AAGCACAAGGTGTGCCTAAA+AGG - chr5_4:15762679-15762698 None:intergenic 45.0%
!!! TTTTTTTTTTTTTTTTGAAA+AGG - chr5_4:15762735-15762754 None:intergenic 5.0%
!!! TTTTTTTTTTTTTTTTGAAA+AGG - chr5_4:15762735-15762754 None:intergenic 5.0%
! CCTTTTTACTGGATCGCCAC+CGG - chr5_4:15760673-15760692 None:intergenic 50.0%
GGGTTCACTTAAGACCTGGT+TGG + chr5_4:15761342-15761361 Msa0840180:intron 50.0%
GTGAGGTAGCTACTCAAGCT+AGG - chr5_4:15761529-15761548 None:intergenic 50.0%
GTGAGGTAGCTACTCAAGCT+AGG - chr5_4:15761529-15761548 None:intergenic 50.0%
CTATGATGCGCAGGTTACTG+TGG - chr5_4:15762153-15762172 None:intergenic 50.0%
CCAGAGCTATATTGGGAGTC+GGG + chr5_4:15762368-15762387 Msa0840180:intron 50.0%
AGGCACACCTTGTGCTTCAA+AGG + chr5_4:15762682-15762701 Msa0840180:three_prime_UTR 50.0%
GGCACACCTTGTGCTTCAAA+GGG + chr5_4:15762683-15762702 Msa0840180:three_prime_UTR 50.0%
! CCTTTTTACTGGATCGCCAC+CGG - chr5_4:15760673-15760692 None:intergenic 50.0%
GGGTTCACTTAAGACCTGGT+TGG + chr5_4:15761342-15761361 Msa0840180:intron 50.0%
GTGAGGTAGCTACTCAAGCT+AGG - chr5_4:15761529-15761548 None:intergenic 50.0%
GTGAGGTAGCTACTCAAGCT+AGG - chr5_4:15761529-15761548 None:intergenic 50.0%
CTATGATGCGCAGGTTACTG+TGG - chr5_4:15762153-15762172 None:intergenic 50.0%
CCAGAGCTATATTGGGAGTC+GGG + chr5_4:15762368-15762387 Msa0840180:intron 50.0%
AGGCACACCTTGTGCTTCAA+AGG + chr5_4:15762682-15762701 Msa0840180:three_prime_UTR 50.0%
GGCACACCTTGTGCTTCAAA+GGG + chr5_4:15762683-15762702 Msa0840180:three_prime_UTR 50.0%
!! CTGAGATGGGCTGAGAGAAG+AGG - chr5_4:15760595-15760614 None:intergenic 55.0%
! GCGCCGACAAATATTACCGG+TGG + chr5_4:15760654-15760673 Msa0840180:CDS 55.0%
CCGGTGGCGATCCAGTAAAA+AGG + chr5_4:15760670-15760689 Msa0840180:CDS 55.0%
GTCAATCCATCCCTCGTGTG+TGG - chr5_4:15761395-15761414 None:intergenic 55.0%
TGAGAGCCATTTGGCTAGCG+AGG + chr5_4:15761921-15761940 Msa0840180:intron 55.0%
CTGCAACCTCGCTAGCCAAA+TGG - chr5_4:15761930-15761949 None:intergenic 55.0%
CCCGACTCCCAATATAGCTC+TGG - chr5_4:15762371-15762390 None:intergenic 55.0%
GAGCTATATTGGGAGTCGGG+TGG + chr5_4:15762371-15762390 Msa0840180:intron 55.0%
! ACGAGTAGCGGAGGAGAAAG+TGG + chr5_4:15762559-15762578 Msa0840180:three_prime_UTR 55.0%
!! CTGAGATGGGCTGAGAGAAG+AGG - chr5_4:15760595-15760614 None:intergenic 55.0%
! GCGCCGACAAATATTACCGG+TGG + chr5_4:15760654-15760673 Msa0840180:CDS 55.0%
CCGGTGGCGATCCAGTAAAA+AGG + chr5_4:15760670-15760689 Msa0840180:CDS 55.0%
GTCAATCCATCCCTCGTGTG+TGG - chr5_4:15761395-15761414 None:intergenic 55.0%
TGAGAGCCATTTGGCTAGCG+AGG + chr5_4:15761921-15761940 Msa0840180:intron 55.0%
CTGCAACCTCGCTAGCCAAA+TGG - chr5_4:15761930-15761949 None:intergenic 55.0%
CCCGACTCCCAATATAGCTC+TGG - chr5_4:15762371-15762390 None:intergenic 55.0%
GAGCTATATTGGGAGTCGGG+TGG + chr5_4:15762371-15762390 Msa0840180:intron 55.0%
! ACGAGTAGCGGAGGAGAAAG+TGG + chr5_4:15762559-15762578 Msa0840180:three_prime_UTR 55.0%
AGAGCCTAGGCCACACACGA+GGG + chr5_4:15761382-15761401 Msa0840180:intron 60.0%
AGAGCCTAGGCCACACACGA+GGG + chr5_4:15761382-15761401 Msa0840180:intron 60.0%
GAGAGCCTAGGCCACACACG+AGG + chr5_4:15761381-15761400 Msa0840180:intron 65.0%
CCATCCCTCGTGTGTGGCCT+AGG - chr5_4:15761389-15761408 None:intergenic 65.0%
CCTAGGCCACACACGAGGGA+TGG + chr5_4:15761386-15761405 Msa0840180:intron 65.0%
GAGAGCCTAGGCCACACACG+AGG + chr5_4:15761381-15761400 Msa0840180:intron 65.0%
CCATCCCTCGTGTGTGGCCT+AGG - chr5_4:15761389-15761408 None:intergenic 65.0%
CCTAGGCCACACACGAGGGA+TGG + chr5_4:15761386-15761405 Msa0840180:intron 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 15760564 15762807 15760564 ID=Msa0840180;Name=Msa0840180
chr5_4 mRNA 15760564 15762807 15760564 ID=Msa0840180-mRNA-1;Parent=Msa0840180;Name=Msa0840180-mRNA-1;_AED=0.00;_eAED=0.00;_QI=74|1|1|1|0|0|2|318|80
chr5_4 exon 15760564 15760762 15760564 ID=Msa0840180-mRNA-1:exon:6733;Parent=Msa0840180-mRNA-1
chr5_4 exon 15762372 15762807 15762372 ID=Msa0840180-mRNA-1:exon:6734;Parent=Msa0840180-mRNA-1
chr5_4 five_prime_UTR 15760564 15760637 15760564 ID=Msa0840180-mRNA-1:five_prime_utr;Parent=Msa0840180-mRNA-1
chr5_4 CDS 15760638 15760762 15760638 ID=Msa0840180-mRNA-1:cds;Parent=Msa0840180-mRNA-1
chr5_4 CDS 15762372 15762489 15762372 ID=Msa0840180-mRNA-1:cds;Parent=Msa0840180-mRNA-1
chr5_4 three_prime_UTR 15762490 15762807 15762490 ID=Msa0840180-mRNA-1:three_prime_utr;Parent=Msa0840180-mRNA-1
Gene Sequence

>Msa0840180

ATGGATGATCAGAAATGCGCCGACAAATATTACCGGTGGCGATCCAGTAAAAAGGAGTATAATAGGTCTCGTCACATTGAATTTGGGATTTTGAGATCTTCTGTTCATAAAATTGGATTTTCAATAGCTATATTGGGAGTCGGGTGGTGTTACTCGCGATTTACTAGCTTGCTGAACCCAGTCGAGACTCAACAGAAAGTCAAACTTTTTAGAGAAAACACTGAAGGATTCAATGAGAACTAA
Protein sequence

>Msa0840180

MDDQKCADKYYRWRSSKKEYNRSRHIEFGILRSSVHKIGFSIAILGVGWCYSRFTSLLNPVETQQKVKLFRENTEGFNEN*