AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1290320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1290320 MtrunA17_Chr8g0364521 94.930 355 18 0 1 355 1 355 0.0 602
Msa1290320 MtrunA17_Chr5g0414271 70.118 338 84 4 17 353 10 331 8.86e-148 420
Msa1290320 MtrunA17_Chr4g0041721 34.393 346 189 7 18 342 6 334 1.29e-55 185
Msa1290320 MtrunA17_Chr5g0412471 40.741 243 136 4 56 294 54 292 1.35e-54 182
Msa1290320 MtrunA17_Chr3g0133211 39.834 241 136 3 50 289 59 291 2.62e-50 172
Msa1290320 MtrunA17_Chr5g0427191 34.409 279 177 3 21 294 11 288 5.29e-47 163
Msa1290320 MtrunA17_Chr1g0208231 35.510 245 145 3 52 294 76 309 7.84e-43 152
Msa1290320 MtrunA17_Chr5g0401271 33.910 289 166 7 21 290 14 296 4.23e-41 147
Msa1290320 MtrunA17_Chr4g0053381 39.234 209 114 3 93 294 81 283 4.82e-41 147
Msa1290320 MtrunA17_Chr3g0134261 33.441 311 155 9 52 339 59 340 1.16e-40 146
Msa1290320 MtrunA17_Chr2g0323361 33.182 220 138 3 75 289 32 247 9.91e-34 126
Msa1290320 MtrunA17_Chr3g0134781 33.115 305 170 8 56 353 31 308 1.40e-32 124
Msa1290320 MtrunA17_Chr2g0280941 35.567 194 118 4 96 289 43 229 1.01e-31 120
Msa1290320 MtrunA17_Chr8g0367661 30.169 295 175 6 21 289 13 302 1.45e-30 119
Msa1290320 MtrunA17_Chr4g0054011 30.818 318 184 8 21 327 6 298 1.58e-25 104
Msa1290320 MtrunA17_Chr3g0102851 30.348 201 125 6 94 288 61 252 9.08e-18 82.4
Msa1290320 MtrunA17_Chr3g0103471 28.922 204 132 5 88 286 45 240 3.21e-16 77.8
Msa1290320 MtrunA17_Chr3g0125041 30.047 213 139 7 41 252 229 432 3.33e-16 79.7
Msa1290320 MtrunA17_Chr3g0102861 28.641 206 134 5 88 288 45 242 6.58e-16 77.0
Msa1290320 MtrunA17_Chr3g0125051 31.694 183 119 4 71 252 252 429 3.04e-15 76.6
Msa1290320 MtrunA17_Chr3g0102621 28.155 206 133 6 89 288 52 248 1.53e-14 73.2
Msa1290320 MtrunA17_Chr5g0441371 29.016 193 121 6 98 286 201 381 1.76e-14 74.3
Msa1290320 MtrunA17_Chr3g0102631 31.148 183 113 5 111 288 1 175 4.07e-14 70.5
Msa1290320 MtrunA17_Chr3g0102651 31.148 183 113 5 111 288 1 175 4.07e-14 70.5
Msa1290320 MtrunA17_Chr3g0102661 31.148 183 113 5 111 288 1 175 4.07e-14 70.5
Msa1290320 MtrunA17_Chr3g0102671 31.148 183 113 5 111 288 1 175 4.07e-14 70.5
Msa1290320 MtrunA17_Chr1g0148681 27.626 257 160 9 14 266 120 354 5.67e-14 72.8
Msa1290320 MtrunA17_Chr3g0134411 27.542 236 144 9 68 292 102 321 7.30e-14 72.0
Msa1290320 MtrunA17_Chr3g0138431 29.500 200 124 6 71 266 225 411 9.94e-14 72.0
Msa1290320 MtrunA17_Chr3g0134401 29.787 188 116 6 71 252 86 263 1.35e-13 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1290320 AT1G70210.1 58.480 342 121 6 11 350 12 334 6.81e-127 368
Msa1290320 AT2G22490.1 40.400 250 146 2 61 310 64 310 2.47e-52 177
Msa1290320 AT2G22490.2 40.800 250 146 1 61 310 64 311 2.70e-51 174
Msa1290320 AT5G65420.4 41.667 216 124 2 50 263 33 248 3.28e-50 169
Msa1290320 AT5G65420.1 41.667 216 124 2 50 263 33 248 4.58e-50 170
Msa1290320 AT5G10440.1 35.088 285 171 4 36 315 7 282 2.29e-48 165
Msa1290320 AT5G65420.3 39.823 226 124 3 50 263 33 258 5.83e-48 165
Msa1290320 AT4G34160.1 39.698 199 120 0 91 289 84 282 4.73e-46 161
Msa1290320 AT3G50070.1 43.523 193 109 0 94 286 86 278 5.41e-45 158
Msa1290320 AT5G67260.1 40.909 198 117 0 91 288 93 290 1.31e-41 149
Msa1290320 AT5G10440.2 38.614 202 119 2 36 232 7 208 1.45e-41 145
Msa1290320 AT5G65420.2 40.816 196 110 3 50 239 33 228 2.17e-41 145
Msa1290320 AT5G67260.2 40.909 198 117 0 91 288 93 290 4.42e-41 149
Msa1290320 AT4G03270.1 35.632 261 155 6 71 322 35 291 9.05e-39 140
Msa1290320 AT4G03270.2 35.632 261 154 7 71 322 35 290 8.73e-37 135
Msa1290320 AT4G37630.2 37.059 170 101 3 83 247 58 226 2.70e-32 122
Msa1290320 AT4G37630.1 32.016 253 157 5 86 324 64 315 2.91e-32 123
Msa1290320 AT4G37630.3 37.349 166 99 3 86 247 64 228 4.07e-31 119
Msa1290320 AT5G02110.1 30.568 229 148 5 61 287 45 264 2.51e-21 94.0
Msa1290320 AT1G77390.1 36.649 191 99 7 71 252 190 367 2.87e-18 85.9
Msa1290320 AT4G37490.1 27.306 271 164 10 30 286 129 380 1.91e-17 83.6
Msa1290320 AT5G02110.2 31.132 212 118 6 89 287 39 235 4.48e-16 78.6
Msa1290320 AT5G11300.2 30.435 230 139 9 41 266 158 370 4.95e-15 76.3
Msa1290320 AT5G11300.1 30.435 230 139 9 41 266 158 370 8.99e-15 75.5
Msa1290320 AT1G44110.1 32.804 189 109 6 71 252 206 383 2.17e-14 74.3
Msa1290320 AT5G25380.4 28.340 247 154 9 26 266 148 377 6.50e-14 72.8
Msa1290320 AT3G11520.1 29.437 231 135 9 89 310 186 397 1.26e-13 72.0
Msa1290320 AT5G43080.2 30.233 215 133 8 40 252 72 271 3.99e-13 70.1
Msa1290320 AT3G11520.2 32.044 181 100 8 89 260 186 352 2.15e-12 68.2
Msa1290320 AT1G80370.1 28.261 230 144 8 41 266 183 395 6.79e-11 63.9

Find 157 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTCTCTCTTGGACTTTC+AGG 0.068778 8_3:-45053612 Msa1290320:intron
CCCCTAGAGGAAGGGGATTT+TGG 0.136284 8_3:-45052420 Msa1290320:three_prime_UTR
GGAAATTAAATAGCTGTTTC+TGG 0.176534 8_3:-45052673 Msa1290320:CDS
GCTGCTTGTTCTGACTATTT+TGG 0.179522 8_3:-45053400 Msa1290320:CDS
TTCATAGTGGCCGGCCTAAT+TGG 0.198111 8_3:-45051799 Msa1290320:three_prime_UTR
ATGCTAGCTTTCTTGCTTAC+AGG 0.207757 8_3:-45053107 Msa1290320:CDS
CCTCGGTTGAAATATGTATT+TGG 0.237116 8_3:+45053440 None:intergenic
CGCAACTGTGGCAACACTTT+AGG 0.262936 8_3:+45052794 None:intergenic
GGCCTAATTGGCTTTGAAAA+TGG 0.263244 8_3:-45051787 Msa1290320:three_prime_UTR
GCAACTGTGGCAACACTTTA+GGG 0.274022 8_3:+45052795 None:intergenic
TTACAGGTACATGAGTATTA+TGG 0.291575 8_3:-45054631 Msa1290320:intron
ATTCAATAATAATATCATTA+TGG 0.292008 8_3:-45051709 Msa1290320:three_prime_UTR
TTTGTTAATCCAAAACGATT+TGG 0.301235 8_3:+45055303 None:intergenic
CACGAGTTCAAGTTCGTTCC+TGG 0.303886 8_3:-45054929 Msa1290320:CDS
ACACTTTAGGGGCATTTCTT+TGG 0.322083 8_3:+45052807 None:intergenic
TTTGGATTCTCGACCTTTAC+CGG 0.322808 8_3:-45054560 Msa1290320:intron
TAAAGGAACAAGAGGAAATA+AGG 0.323576 8_3:-45052593 Msa1290320:three_prime_UTR
TTCATTAGCTGCAGAGAGTA+TGG 0.323783 8_3:+45053062 None:intergenic
CATTGAAATTTGAAACCATA+TGG 0.324813 8_3:-45055422 Msa1290320:five_prime_UTR
GTATGAGAGTTTGATGGTTT+TGG 0.331469 8_3:-45052499 Msa1290320:three_prime_UTR
TCAGAAAAGTCCATGAAATA+TGG 0.332429 8_3:+45052063 None:intergenic
CAGCTAATGAAATTCCTAAT+TGG 0.339802 8_3:-45053050 Msa1290320:CDS
AATGGAGGTGAAAAGGTCTT+TGG 0.342231 8_3:-45051769 Msa1290320:three_prime_UTR
GTACCTCTCCGTTAACTATA+TGG 0.343791 8_3:-45054590 Msa1290320:CDS
CCATGCAATGGCTTCTTCTC+TGG 0.345477 8_3:+45054859 None:intergenic
ACACACATTTATTGATGTAT+AGG 0.347613 8_3:-45052396 Msa1290320:three_prime_UTR
TTAAAGTTGGCCATATTTCA+TGG 0.348454 8_3:-45052073 Msa1290320:three_prime_UTR
TTTCTGTTGTTTATTGTTGA+GGG 0.353681 8_3:+45052100 None:intergenic
CGAGACACTGGACCACCTTC+TGG 0.356767 8_3:+45052757 None:intergenic
GTATCAAAATCAAGACCTTT+GGG 0.365741 8_3:-45052240 Msa1290320:three_prime_UTR
ATGGAGGTGAAAAGGTCTTT+GGG 0.373374 8_3:-45051768 Msa1290320:three_prime_UTR
GGATTAACAAAAGACCAATT+AGG 0.379119 8_3:+45053036 None:intergenic
GGTATCAAAATCAAGACCTT+TGG 0.387161 8_3:-45052241 Msa1290320:three_prime_UTR
TCAAATCAACTGACCCCATA+TGG 0.387381 8_3:+45055407 None:intergenic
ATTTCTGTTGTTTATTGTTG+AGG 0.388407 8_3:+45052099 None:intergenic
AATGGCTTCTTCTCTGGTGC+TGG 0.389156 8_3:+45054865 None:intergenic
ACCACTGGTTCCTTCTCTCT+TGG 0.394396 8_3:-45053621 Msa1290320:CDS
ATAAATATGCGCGGCCTCAT+GGG 0.409258 8_3:+45055239 None:intergenic
GCGTGCAAGCTAGATTCAAC+TGG 0.413916 8_3:-45053330 Msa1290320:CDS
TATAAATATGCGCGGCCTCA+TGG 0.415439 8_3:+45055238 None:intergenic
TCGAGGTCGGAGCAGCATTC+CGG 0.422894 8_3:+45055031 None:intergenic
CATTGAAGTCCTGTATAGTT+AGG 0.423009 8_3:+45051925 None:intergenic
AATTACAAGAATAATAATAA+AGG 0.425909 8_3:-45052610 Msa1290320:three_prime_UTR
AAACCGATCCATATAGTTAA+CGG 0.429067 8_3:+45054582 None:intergenic
GAGGGTGAAGAGGAGGAAGA+TGG 0.432218 8_3:+45052719 None:intergenic
GAAATTAAATAGCTGTTTCT+GGG 0.434497 8_3:-45052672 Msa1290320:CDS
AATGAATGACGAGCAATTAC+CGG 0.439363 8_3:-45055137 Msa1290320:CDS
TTTGTCTTTAGCAGCAAAGA+TGG 0.440763 8_3:-45053648 Msa1290320:CDS
CTATACAGGACTTCAATGTT+TGG 0.446478 8_3:-45051920 Msa1290320:three_prime_UTR
TCAAAGGTGGCATTGATCAT+AGG 0.447393 8_3:-45052317 Msa1290320:three_prime_UTR
TTGTATGTATGAGAGTTTGA+TGG 0.449102 8_3:-45052505 Msa1290320:three_prime_UTR
AAACAAGAGCTCACCGGTAA+AGG 0.452049 8_3:+45054547 None:intergenic
TCCAAAATCCCCTTCCTCTA+GGG 0.453953 8_3:+45052419 None:intergenic
ACCGAGGACGATTCTTAGAA+TGG 0.457634 8_3:-45053424 Msa1290320:CDS
CCACATTAACAGGAATCAAA+TGG 0.460281 8_3:-45053701 Msa1290320:intron
ACGGAGAGGTACGCCGTTAA+CGG 0.466512 8_3:+45054601 None:intergenic
TTATGGATTTCAGCCGTTAA+CGG 0.467222 8_3:-45054614 Msa1290320:CDS
TGGCTTCGAGAGAGCGAGAT+TGG 0.470113 8_3:+45054885 None:intergenic
ATCACACAAACACACATGAT+TGG 0.472494 8_3:+45052010 None:intergenic
CTTTGAAAATGGAGGTGAAA+AGG 0.474300 8_3:-45051776 Msa1290320:three_prime_UTR
ATTGAAATTTGAAACCATAT+GGG 0.475789 8_3:-45055421 Msa1290320:five_prime_UTR
GTCATTATTCGCTGAGGAAG+AGG 0.482928 8_3:-45054978 Msa1290320:CDS
GAGGTTTGGCCTAACTATAC+AGG 0.491757 8_3:-45051934 Msa1290320:three_prime_UTR
CACCATGACTCAGCATGCTC+AGG 0.491771 8_3:+45053015 None:intergenic
TCTGATAGTACGTGTGAAGA+TGG 0.492912 8_3:-45052045 Msa1290320:three_prime_UTR
TTCCAAAATCCCCTTCCTCT+AGG 0.496813 8_3:+45052418 None:intergenic
AAGAGGAAATAAGGTGGGCC+AGG 0.497493 8_3:-45052584 Msa1290320:three_prime_UTR
GTGGGATGAAACAGACAAAT+CGG 0.499191 8_3:+45055488 None:intergenic
GCAGCATTCCGGTGAATCTC+CGG 0.501366 8_3:+45055042 None:intergenic
TGATGACGACGACGAGACAC+TGG 0.503625 8_3:+45052745 None:intergenic
TGTTGAGGGTATCCCTTCAA+AGG 0.504042 8_3:+45052114 None:intergenic
CAACAGAAATGAGTTAAAGT+TGG 0.517497 8_3:-45052086 Msa1290320:three_prime_UTR
ATATAAATCATGTGACTCAC+GGG 0.522420 8_3:-45051636 Msa1290320:three_prime_UTR
AATACACAGCACAGAGAAGA+AGG 0.522844 8_3:+45051744 None:intergenic
AATATAAATCATGTGACTCA+CGG 0.523855 8_3:-45051637 Msa1290320:three_prime_UTR
AAAGAAAGAAAAGATTGAAG+AGG 0.524980 8_3:-45055336 Msa1290320:five_prime_UTR
TTTATACGACAACGGGAAAG+AGG 0.530227 8_3:+45052700 None:intergenic
GACTCGTCGGAAGTCCTCAC+CGG 0.532194 8_3:-45055061 Msa1290320:CDS
AGCAAAGATGGAGGAACCAC+TGG 0.536634 8_3:-45053636 Msa1290320:CDS
AGTCCTCACCGGAGATTCAC+CGG 0.539749 8_3:-45055050 Msa1290320:CDS
AATAATAATAAAGGAACAAG+AGG 0.541276 8_3:-45052601 Msa1290320:three_prime_UTR
TATTATAAACTGTAGGTTGA+AGG 0.547046 8_3:-45053465 Msa1290320:intron
TACCTTGAGAATCCATGCAA+TGG 0.548867 8_3:+45054847 None:intergenic
CAACGGGAAAGAGGGTGAAG+AGG 0.550019 8_3:+45052709 None:intergenic
TGACGTGGTAATAATTGTTG+AGG 0.550422 8_3:-45052348 Msa1290320:three_prime_UTR
CGGGAAAGAGGGTGAAGAGG+AGG 0.552466 8_3:+45052712 None:intergenic
GACAGTGGCATGAGAATCAC+CGG 0.553646 8_3:+45055118 None:intergenic
TTGGTGTCCAACGCGTGTGA+TGG 0.554163 8_3:-45051901 Msa1290320:three_prime_UTR
GCCGCAGAGGAGCTCGCAGT+CGG 0.555940 8_3:+45055087 None:intergenic
TGGTGTCCAACGCGTGTGAT+GGG 0.556297 8_3:-45051900 Msa1290320:three_prime_UTR
CACAGTTGCGAGTTACAGCT+CGG 0.556570 8_3:-45052781 Msa1290320:CDS
TAACTGTATCCCCTAGAGGA+AGG 0.559488 8_3:-45052429 Msa1290320:three_prime_UTR
ACTGTATCCCCTAGAGGAAG+GGG 0.560583 8_3:-45052427 Msa1290320:three_prime_UTR
CTGTGGGCACACTGGACATA+CGG 0.561667 8_3:+45055367 None:intergenic
CCAGAGAAGAAGCCATTGCA+TGG 0.564052 8_3:-45054859 Msa1290320:CDS
AACTGTATCCCCTAGAGGAA+GGG 0.564428 8_3:-45052428 Msa1290320:three_prime_UTR
AAGAAACTGAGGAAACTAAG+TGG 0.566911 8_3:+45053354 None:intergenic
GCAGAGGAGCTCGCAGTCGG+AGG 0.568318 8_3:+45055090 None:intergenic
TGTTGAGGATATGAATTCAA+AGG 0.570658 8_3:-45052333 Msa1290320:three_prime_UTR
AGTACGTGTGAAGATGGCTT+TGG 0.572878 8_3:-45052039 Msa1290320:three_prime_UTR
AAAATAAAGATACTCAATGT+GGG 0.574252 8_3:+45055470 None:intergenic
TGATCATAGGTGAAAGATGG+TGG 0.575297 8_3:-45052304 Msa1290320:three_prime_UTR
ATCATAGGTGAAAGATGGTG+GGG 0.575499 8_3:-45052302 Msa1290320:three_prime_UTR
ACCTGAAAGTCCAAGAGAGA+AGG 0.578425 8_3:+45053611 None:intergenic
TTTCTGTAGCACGTGAAATG+AGG 0.580429 8_3:+45053292 None:intergenic
TATAGTTAGGCCAAACCTCA+TGG 0.581514 8_3:+45051938 None:intergenic
ATCATCTTATCTAATATCCA+AGG 0.583000 8_3:-45053270 Msa1290320:intron
GATCATAGGTGAAAGATGGT+GGG 0.583747 8_3:-45052303 Msa1290320:three_prime_UTR
CCAAAATCCCCTTCCTCTAG+GGG 0.585025 8_3:+45052420 None:intergenic
AGAAACTGAGGAAACTAAGT+GGG 0.585998 8_3:+45053355 None:intergenic
CTTGAGACGTATTCGAAACC+AGG 0.587887 8_3:+45054911 None:intergenic
CTAATTGGCTTTGAAAATGG+AGG 0.589070 8_3:-45051784 Msa1290320:three_prime_UTR
TTGAAATTTGAAACCATATG+GGG 0.593011 8_3:-45055420 Msa1290320:five_prime_UTR
TCCAAGAGAGAAGGAACCAG+TGG 0.594281 8_3:+45053620 None:intergenic
TTATACGACAACGGGAAAGA+GGG 0.599485 8_3:+45052701 None:intergenic
TCTCCGTTAACTATATGGAT+CGG 0.601291 8_3:-45054585 Msa1290320:CDS
CAAAATAAAGATACTCAATG+TGG 0.602917 8_3:+45055469 None:intergenic
CCTCTGCGGCGAAGACTCGT+CGG 0.603626 8_3:-45055074 Msa1290320:CDS
GCAGCTGCAGCAATGCATGA+TGG 0.607146 8_3:+45053084 None:intergenic
CAGTCGGAGGTGCTTGACAG+TGG 0.607390 8_3:+45055103 None:intergenic
GTTGAGGTTTCTAGACAACC+TGG 0.608930 8_3:+45052566 None:intergenic
GTCTTTAGCAGCAAAGATGG+AGG 0.609267 8_3:-45053645 Msa1290320:CDS
GAGTTACAGCTCGGACCAGA+AGG 0.609369 8_3:-45052772 Msa1290320:CDS
ATCCTGAGCATGCTGAGTCA+TGG 0.611842 8_3:-45053017 Msa1290320:CDS
AATAATGACGACAACGGCAA+CGG 0.617108 8_3:+45054992 None:intergenic
TCCATTCTAAGAATCGTCCT+CGG 0.617659 8_3:+45053423 None:intergenic
CAACTGTGGCAACACTTTAG+GGG 0.621242 8_3:+45052796 None:intergenic
CATTGATCATAGGTGAAAGA+TGG 0.623465 8_3:-45052307 Msa1290320:three_prime_UTR
AAATGAGTGATGTTATTGCT+TGG 0.624228 8_3:+45052138 None:intergenic
ATTAACAGGAATCAAATGGA+TGG 0.627209 8_3:-45053697 Msa1290320:intron
CATGCTGAGTCATGGTGTGA+AGG 0.628250 8_3:-45053009 Msa1290320:CDS
GTGTTTCCCATCACACGCGT+TGG 0.632047 8_3:+45051894 None:intergenic
GGAACAAGAGGAAATAAGGT+GGG 0.638904 8_3:-45052589 Msa1290320:three_prime_UTR
GCAACAGATAAAAGTTGCAG+TGG 0.643230 8_3:+45053674 None:intergenic
TGAGGATATGAATTCAAAGG+TGG 0.647355 8_3:-45052330 Msa1290320:three_prime_UTR
AGCCATTGCATGGATTCTCA+AGG 0.647866 8_3:-45054849 Msa1290320:intron
GGTGTAACTGTATCCCCTAG+AGG 0.650922 8_3:-45052433 Msa1290320:three_prime_UTR
TATCAAAATCAAGACCTTTG+GGG 0.657692 8_3:-45052239 Msa1290320:three_prime_UTR
ATTATTCGCTGAGGAAGAGG+AGG 0.658872 8_3:-45054975 Msa1290320:CDS
AGGAACAAGAGGAAATAAGG+TGG 0.659702 8_3:-45052590 Msa1290320:three_prime_UTR
CGAGCTGTAACTCGCAACTG+TGG 0.662417 8_3:+45052782 None:intergenic
TCATAGGTGAAAGATGGTGG+GGG 0.667992 8_3:-45052301 Msa1290320:three_prime_UTR
TTACAGCTCGGACCAGAAGG+TGG 0.668081 8_3:-45052769 Msa1290320:CDS
TGTTTCACTATAAATATGCG+CGG 0.671202 8_3:+45055230 None:intergenic
GTTGTCGTCATTATTCGCTG+AGG 0.673229 8_3:-45054984 Msa1290320:CDS
TGGAGGTGAAAAGGTCTTTG+GGG 0.673307 8_3:-45051767 Msa1290320:three_prime_UTR
GCTTGCACGCAAAGAAACTG+AGG 0.675792 8_3:+45053343 None:intergenic
TTCTGGGTAGATGATGACAA+AGG 0.678492 8_3:-45052656 Msa1290320:CDS
CCGACGAGTCTTCGCCGCAG+AGG 0.679065 8_3:+45055074 None:intergenic
TGATGATGATGAATCGAGGT+CGG 0.693359 8_3:+45055018 None:intergenic
CCAAATACATATTTCAACCG+AGG 0.701316 8_3:-45053440 Msa1290320:CDS
TCCGACTGCGAGCTCCTCTG+CGG 0.715990 8_3:-45055088 Msa1290320:CDS
GAAACTGAGGAAACTAAGTG+GGG 0.716216 8_3:+45053356 None:intergenic
ATTCCGGTGAATCTCCGGTG+AGG 0.721007 8_3:+45055047 None:intergenic
AGAGAGAGAAGCATTTGACG+TGG 0.723209 8_3:-45052363 Msa1290320:three_prime_UTR
TCAGCGAATAATGACGACAA+CGG 0.723840 8_3:+45054986 None:intergenic
GATCCATATAGTTAACGGAG+AGG 0.735766 8_3:+45054587 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTATTAACAATTGAAAAA+TGG - chr8_3:45051856-45051875 Msa1290320:three_prime_UTR 10.0%
!!! AAATAATTTTAAAACTATGA+TGG - chr8_3:45053923-45053942 Msa1290320:intron 10.0%
!! AATTACAAGAATAATAATAA+AGG - chr8_3:45054512-45054531 Msa1290320:intron 10.0%
!! ATAAAAACGAAAAATAAAAT+AGG + chr8_3:45054663-45054682 None:intergenic 10.0%
!!! ATACTTTTACATTTTTTTAT+TGG - chr8_3:45055274-45055293 Msa1290320:five_prime_UTR 10.0%
!! ATTCAATAATAATATCATTA+TGG - chr8_3:45055413-45055432 Msa1290320:five_prime_UTR 10.0%
!! GATTTATCAAAATTTATTAC+TGG + chr8_3:45052625-45052644 None:intergenic 15.0%
!!! GATAAATCATTTTTTTTTGA+AGG - chr8_3:45052637-45052656 Msa1290320:exon 15.0%
!! TTTGAATATGATAATATTAG+TGG - chr8_3:45053344-45053363 Msa1290320:CDS 15.0%
!! TTTATTGAACAACTATATTA+TGG - chr8_3:45053381-45053400 Msa1290320:CDS 15.0%
!! TTAGTATTATTATCACTATA+TGG + chr8_3:45053614-45053633 None:intergenic 15.0%
!! TAATGTATATTATAAACTGT+AGG - chr8_3:45053650-45053669 Msa1290320:CDS 15.0%
!!! ATTTTTCGTTTTTATCTTTT+TGG - chr8_3:45054668-45054687 Msa1290320:intron 15.0%
!! AAAATAAAGATACTCAATGT+GGG + chr8_3:45051655-45051674 None:intergenic 20.0%
!!! ATTGAAATTTGAAACCATAT+GGG - chr8_3:45051701-45051720 Msa1290320:three_prime_UTR 20.0%
!! TAAAAAATAAACAAAGCAGT+TGG + chr8_3:45052474-45052493 None:intergenic 20.0%
!!! GATAAATCATTTTGTTTTCT+CGG - chr8_3:45052668-45052687 Msa1290320:CDS 20.0%
!! ATAATAATTGTGATACTGAA+GGG + chr8_3:45052817-45052836 None:intergenic 20.0%
!!! ATACAGTATTTTTGTAGTAT+AGG + chr8_3:45053182-45053201 None:intergenic 20.0%
!! AGGTATACATTCATTATTTA+AGG - chr8_3:45053530-45053549 Msa1290320:intron 20.0%
!! TTATTGCAGGAAAAAATTAT+AGG - chr8_3:45054253-45054272 Msa1290320:intron 20.0%
!! TATTGCAGGAAAAAATTATA+GGG - chr8_3:45054254-45054273 Msa1290320:intron 20.0%
!! AATAATAATAAAGGAACAAG+AGG - chr8_3:45054521-45054540 Msa1290320:intron 20.0%
!!! TGTGATTTTTTGTTTGTTTT+TGG - chr8_3:45055129-45055148 Msa1290320:CDS 20.0%
!!! GTGATTTTTTGTTTGTTTTT+GGG - chr8_3:45055130-45055149 Msa1290320:CDS 20.0%
!!! TTTATTGGTTTTGAAAAAAG+AGG - chr8_3:45055289-45055308 Msa1290320:five_prime_UTR 20.0%
! CAAAATAAAGATACTCAATG+TGG + chr8_3:45051656-45051675 None:intergenic 25.0%
!! CATTGAAATTTGAAACCATA+TGG - chr8_3:45051700-45051719 Msa1290320:three_prime_UTR 25.0%
!! TTGAAATTTGAAACCATATG+GGG - chr8_3:45051702-45051721 Msa1290320:three_prime_UTR 25.0%
! AAAGAAAGAAAAGATTGAAG+AGG - chr8_3:45051786-45051805 Msa1290320:three_prime_UTR 25.0%
! TTTGTTAATCCAAAACGATT+TGG + chr8_3:45051822-45051841 None:intergenic 25.0%
! AAGGTAAATAGAAACAGTAA+AGG - chr8_3:45052292-45052311 Msa1290320:three_prime_UTR 25.0%
!!! CATTTTAGCATAAAGTAGTT+CGG + chr8_3:45052337-45052356 None:intergenic 25.0%
!!! CTGCTTTGTTTATTTTTTAC+AGG - chr8_3:45052475-45052494 Msa1290320:three_prime_UTR 25.0%
! CATAATAATTGTGATACTGA+AGG + chr8_3:45052818-45052837 None:intergenic 25.0%
! TCATTCATAGAATTTGTCAA+TGG + chr8_3:45053038-45053057 None:intergenic 25.0%
! AATTACATTACCACATTAAC+AGG - chr8_3:45053411-45053430 Msa1290320:CDS 25.0%
!! TATTATAAACTGTAGGTTGA+AGG - chr8_3:45053657-45053676 Msa1290320:CDS 25.0%
! ATCATCTTATCTAATATCCA+AGG - chr8_3:45053852-45053871 Msa1290320:intron 25.0%
! CATAGTACTATAGTAGTATA+CGG - chr8_3:45054173-45054192 Msa1290320:intron 25.0%
! ATAGTACTATAGTAGTATAC+GGG - chr8_3:45054174-45054193 Msa1290320:intron 25.0%
! GTAAATGAAAGAAAATCATG+TGG - chr8_3:45054196-45054215 Msa1290320:intron 25.0%
! ATAAACAGATATATGTGCTT+TGG + chr8_3:45054237-45054256 None:intergenic 25.0%
!!! GAAATTAAATAGCTGTTTCT+GGG - chr8_3:45054450-45054469 Msa1290320:intron 25.0%
! ACACACATTTATTGATGTAT+AGG - chr8_3:45054726-45054745 Msa1290320:intron 25.0%
!! TTTCTGTTGTTTATTGTTGA+GGG + chr8_3:45055025-45055044 None:intergenic 25.0%
!! ATTTCTGTTGTTTATTGTTG+AGG + chr8_3:45055026-45055045 None:intergenic 25.0%
!! ATTTTCTGATCCCTTTTTTT+AGG - chr8_3:45055161-45055180 Msa1290320:five_prime_UTR 25.0%
! AATATAAATCATGTGACTCA+CGG - chr8_3:45055485-45055504 Msa1290320:five_prime_UTR 25.0%
!! CTTTTTCATCCAAATCGTTT+TGG - chr8_3:45051810-45051829 Msa1290320:three_prime_UTR 30.0%
TGTTTCACTATAAATATGCG+CGG + chr8_3:45051895-45051914 None:intergenic 30.0%
TTACAGGTACATGAGTATTA+TGG - chr8_3:45052491-45052510 Msa1290320:three_prime_UTR 30.0%
AAACCGATCCATATAGTTAA+CGG + chr8_3:45052543-45052562 None:intergenic 30.0%
!! TAACTATATGGATCGGTTTT+TGG - chr8_3:45052544-45052563 Msa1290320:three_prime_UTR 30.0%
AAAAAAAAACAAGAGCTCAC+CGG + chr8_3:45052584-45052603 None:intergenic 30.0%
GTATCACAATTATTATGCCA+AGG - chr8_3:45052821-45052840 Msa1290320:CDS 30.0%
TAACTGTGAATAGAAAACCT+TGG + chr8_3:45052841-45052860 None:intergenic 30.0%
ATTAACAGGAATCAAATGGA+TGG - chr8_3:45053425-45053444 Msa1290320:CDS 30.0%
AGAACCCCATGTTTATTATT+CGG - chr8_3:45053579-45053598 Msa1290320:intron 30.0%
ACATGCCGAATAATAAACAT+GGG + chr8_3:45053587-45053606 None:intergenic 30.0%
!! TTGTTCTGACTATTTTGGAT+TGG - chr8_3:45053727-45053746 Msa1290320:intron 30.0%
TTAAGGGTAAAAGAATACCT+TGG + chr8_3:45053872-45053891 None:intergenic 30.0%
!! CAAGCATTGAGAGAAATTAA+GGG + chr8_3:45053888-45053907 None:intergenic 30.0%
!! TCAAGCATTGAGAGAAATTA+AGG + chr8_3:45053889-45053908 None:intergenic 30.0%
! ATCTCTATTGTGAGTTTTCA+AGG - chr8_3:45053959-45053978 Msa1290320:intron 30.0%
CAGCTAATGAAATTCCTAAT+TGG - chr8_3:45054072-45054091 Msa1290320:intron 30.0%
GGATTAACAAAAGACCAATT+AGG + chr8_3:45054089-45054108 None:intergenic 30.0%
CACATATATCTGTTTATTGC+AGG - chr8_3:45054240-45054259 Msa1290320:intron 30.0%
! TTCCTCTTTTTATACGACAA+CGG + chr8_3:45054433-45054452 None:intergenic 30.0%
! GGAAATTAAATAGCTGTTTC+TGG - chr8_3:45054449-45054468 Msa1290320:intron 30.0%
TAAAGGAACAAGAGGAAATA+AGG - chr8_3:45054529-45054548 Msa1290320:intron 30.0%
! CTAGAAACCTCAACATTTTT+AGG - chr8_3:45054565-45054584 Msa1290320:CDS 30.0%
!! AAACCTCAACATTTTTAGGA+GGG - chr8_3:45054569-45054588 Msa1290320:CDS 30.0%
TTGTATGTATGAGAGTTTGA+TGG - chr8_3:45054617-45054636 Msa1290320:CDS 30.0%
TGTTGAGGATATGAATTCAA+AGG - chr8_3:45054789-45054808 Msa1290320:intron 30.0%
GTATCAAAATCAAGACCTTT+GGG - chr8_3:45054882-45054901 Msa1290320:CDS 30.0%
TATCAAAATCAAGACCTTTG+GGG - chr8_3:45054883-45054902 Msa1290320:CDS 30.0%
! ATGAGTGAAGTTTCTTTTCT+TGG - chr8_3:45054959-45054978 Msa1290320:CDS 30.0%
AAATGAGTGATGTTATTGCT+TGG + chr8_3:45054987-45055006 None:intergenic 30.0%
! ATCACTCATTTTCCTTTGAA+GGG - chr8_3:45054996-45055015 Msa1290320:CDS 30.0%
CAACAGAAATGAGTTAAAGT+TGG - chr8_3:45055036-45055055 Msa1290320:CDS 30.0%
TTAAAGTTGGCCATATTTCA+TGG - chr8_3:45055049-45055068 Msa1290320:CDS 30.0%
TCAGAAAAGTCCATGAAATA+TGG + chr8_3:45055062-45055081 None:intergenic 30.0%
ATATAAATCATGTGACTCAC+GGG - chr8_3:45055486-45055505 Msa1290320:five_prime_UTR 30.0%
! CTTTCTTTCTTTTGTGCTGT+GGG + chr8_3:45051774-45051793 None:intergenic 35.0%
! TCTTTCTTTCTTTTGTGCTG+TGG + chr8_3:45051775-45051794 None:intergenic 35.0%
!! GAGAAATTTTGTAGGTTGGA+AGG + chr8_3:45051928-45051947 None:intergenic 35.0%
! GAGAGAGAAATTTTGTAGGT+TGG + chr8_3:45051932-45051951 None:intergenic 35.0%
! GAGAGAGAGAGAAATTTTGT+AGG + chr8_3:45051936-45051955 None:intergenic 35.0%
AATGAATGACGAGCAATTAC+CGG - chr8_3:45051985-45052004 Msa1290320:three_prime_UTR 35.0%
TTATGGATTTCAGCCGTTAA+CGG - chr8_3:45052508-45052527 Msa1290320:three_prime_UTR 35.0%
TCTCCGTTAACTATATGGAT+CGG - chr8_3:45052537-45052556 Msa1290320:three_prime_UTR 35.0%
TGACATACATGTGGAAAAGA+GGG + chr8_3:45053300-45053319 None:intergenic 35.0%
ATGTCACGTGAGTGTATTTA+TGG - chr8_3:45053314-45053333 Msa1290320:CDS 35.0%
CCATTTGATTCCTGTTAATG+TGG + chr8_3:45053424-45053443 None:intergenic 35.0%
CCACATTAACAGGAATCAAA+TGG - chr8_3:45053421-45053440 Msa1290320:CDS 35.0%
TTTGTCTTTAGCAGCAAAGA+TGG - chr8_3:45053474-45053493 Msa1290320:intron 35.0%
CATGCCGAATAATAAACATG+GGG + chr8_3:45053586-45053605 None:intergenic 35.0%
GACATGCCGAATAATAAACA+TGG + chr8_3:45053588-45053607 None:intergenic 35.0%
CCTCGGTTGAAATATGTATT+TGG + chr8_3:45053685-45053704 None:intergenic 35.0%
CCAAATACATATTTCAACCG+AGG - chr8_3:45053682-45053701 Msa1290320:CDS 35.0%
!! TTCTGACTATTTTGGATTGG+AGG - chr8_3:45053730-45053749 Msa1290320:intron 35.0%
AGAAACTGAGGAAACTAAGT+GGG + chr8_3:45053770-45053789 None:intergenic 35.0%
AAGAAACTGAGGAAACTAAG+TGG + chr8_3:45053771-45053790 None:intergenic 35.0%
! TCCTCTTTTTATACGACAAC+GGG + chr8_3:45054432-45054451 None:intergenic 35.0%
!! GAAACCTCAACATTTTTAGG+AGG - chr8_3:45054568-45054587 Msa1290320:CDS 35.0%
AAACCCTCCTAAAAATGTTG+AGG + chr8_3:45054575-45054594 None:intergenic 35.0%
!! GTATGAGAGTTTGATGGTTT+TGG - chr8_3:45054623-45054642 Msa1290320:CDS 35.0%
TGACGTGGTAATAATTGTTG+AGG - chr8_3:45054774-45054793 Msa1290320:intron 35.0%
TGAGGATATGAATTCAAAGG+TGG - chr8_3:45054792-45054811 Msa1290320:intron 35.0%
CATTGATCATAGGTGAAAGA+TGG - chr8_3:45054815-45054834 Msa1290320:intron 35.0%
GGTATCAAAATCAAGACCTT+TGG - chr8_3:45054881-45054900 Msa1290320:CDS 35.0%
!!! AGCTGTTTTTGACTTGTCAT+GGG - chr8_3:45054926-45054945 Msa1290320:CDS 35.0%
! CATCACTCATTTTCCTTTGA+AGG - chr8_3:45054995-45055014 Msa1290320:CDS 35.0%
ATCACACAAACACACATGAT+TGG + chr8_3:45055115-45055134 None:intergenic 35.0%
AACCTCATGGCCTAAAAAAA+GGG + chr8_3:45055174-45055193 None:intergenic 35.0%
AAACCTCATGGCCTAAAAAA+AGG + chr8_3:45055175-45055194 None:intergenic 35.0%
!!! TTTTTTTAGGCCATGAGGTT+TGG - chr8_3:45055174-45055193 Msa1290320:five_prime_UTR 35.0%
CATTGAAGTCCTGTATAGTT+AGG + chr8_3:45055200-45055219 None:intergenic 35.0%
CTATACAGGACTTCAATGTT+TGG - chr8_3:45055202-45055221 Msa1290320:five_prime_UTR 35.0%
! ATTTTCAAAGCCAATTAGGC+CGG + chr8_3:45055336-45055355 None:intergenic 35.0%
! CTCCATTTTCAAAGCCAATT+AGG + chr8_3:45055340-45055359 None:intergenic 35.0%
!! CTAATTGGCTTTGAAAATGG+AGG - chr8_3:45055338-45055357 Msa1290320:five_prime_UTR 35.0%
! CTTTGAAAATGGAGGTGAAA+AGG - chr8_3:45055346-45055365 Msa1290320:five_prime_UTR 35.0%
GTGGGATGAAACAGACAAAT+CGG + chr8_3:45051637-45051656 None:intergenic 40.0%
TCAAATCAACTGACCCCATA+TGG + chr8_3:45051718-45051737 None:intergenic 40.0%
TGAAAAATGGAAACCCCATG+AGG - chr8_3:45051869-45051888 Msa1290320:three_prime_UTR 40.0%
AATTTCTCTCTCTCTCTCTC+TGG - chr8_3:45051940-45051959 Msa1290320:three_prime_UTR 40.0%
! TGATGATGATGAATCGAGGT+CGG + chr8_3:45052107-45052126 None:intergenic 40.0%
! GTGATGATGATGATGAATCG+AGG + chr8_3:45052111-45052130 None:intergenic 40.0%
AATAATGACGACAACGGCAA+CGG + chr8_3:45052133-45052152 None:intergenic 40.0%
TCAGCGAATAATGACGACAA+CGG + chr8_3:45052139-45052158 None:intergenic 40.0%
TACCTTGAGAATCCATGCAA+TGG + chr8_3:45052278-45052297 None:intergenic 40.0%
!! TTAACAGCGTCGATGAATCA+TGG - chr8_3:45052392-45052411 Msa1290320:three_prime_UTR 40.0%
GTACCTCTCCGTTAACTATA+TGG - chr8_3:45052532-45052551 Msa1290320:three_prime_UTR 40.0%
GATCCATATAGTTAACGGAG+AGG + chr8_3:45052538-45052557 None:intergenic 40.0%
TTTGGATTCTCGACCTTTAC+CGG - chr8_3:45052562-45052581 Msa1290320:three_prime_UTR 40.0%
! CTTTTGACACTTCCAATACG+AGG - chr8_3:45052739-45052758 Msa1290320:CDS 40.0%
GTGACATACATGTGGAAAAG+AGG + chr8_3:45053301-45053320 None:intergenic 40.0%
GCAACAGATAAAAGTTGCAG+TGG + chr8_3:45053451-45053470 None:intergenic 40.0%
! TCCATTCTAAGAATCGTCCT+CGG + chr8_3:45053702-45053721 None:intergenic 40.0%
! GCTGCTTGTTCTGACTATTT+TGG - chr8_3:45053722-45053741 Msa1290320:intron 40.0%
GAAACTGAGGAAACTAAGTG+GGG + chr8_3:45053769-45053788 None:intergenic 40.0%
TTTCTGTAGCACGTGAAATG+AGG + chr8_3:45053833-45053852 None:intergenic 40.0%
!! ATGCTAGCTTTCTTGCTTAC+AGG - chr8_3:45054015-45054034 Msa1290320:intron 40.0%
TTCATTAGCTGCAGAGAGTA+TGG + chr8_3:45054063-45054082 None:intergenic 40.0%
!! ACACTTTAGGGGCATTTCTT+TGG + chr8_3:45054318-45054337 None:intergenic 40.0%
TTATACGACAACGGGAAAGA+GGG + chr8_3:45054424-45054443 None:intergenic 40.0%
TTTATACGACAACGGGAAAG+AGG + chr8_3:45054425-45054444 None:intergenic 40.0%
TCCCGTTGTCGTATAAAAAG+AGG - chr8_3:45054428-45054447 Msa1290320:intron 40.0%
TTCTGGGTAGATGATGACAA+AGG - chr8_3:45054466-45054485 Msa1290320:intron 40.0%
AGGAACAAGAGGAAATAAGG+TGG - chr8_3:45054532-45054551 Msa1290320:intron 40.0%
GGAACAAGAGGAAATAAGGT+GGG - chr8_3:45054533-45054552 Msa1290320:intron 40.0%
!! TCAAAGGTGGCATTGATCAT+AGG - chr8_3:45054805-45054824 Msa1290320:intron 40.0%
TGATCATAGGTGAAAGATGG+TGG - chr8_3:45054818-45054837 Msa1290320:intron 40.0%
GATCATAGGTGAAAGATGGT+GGG - chr8_3:45054819-45054838 Msa1290320:intron 40.0%
ATCATAGGTGAAAGATGGTG+GGG - chr8_3:45054820-45054839 Msa1290320:intron 40.0%
GTGAAGAAAAAACAGAGAGC+AGG - chr8_3:45054860-45054879 Msa1290320:CDS 40.0%
! TTCAGAGTTTTCTTCCCCAA+AGG + chr8_3:45054900-45054919 None:intergenic 40.0%
!!! GAGCTGTTTTTGACTTGTCA+TGG - chr8_3:45054925-45054944 Msa1290320:CDS 40.0%
TCTGATAGTACGTGTGAAGA+TGG - chr8_3:45055077-45055096 Msa1290320:CDS 40.0%
!!! ATCCCTTTTTTTAGGCCATG+AGG - chr8_3:45055169-45055188 Msa1290320:five_prime_UTR 40.0%
TATAGTTAGGCCAAACCTCA+TGG + chr8_3:45055187-45055206 None:intergenic 40.0%
! GGTGAACTCTCTTTTCATAG+TGG - chr8_3:45055310-45055329 Msa1290320:five_prime_UTR 40.0%
! AACTCTCTTTTCATAGTGGC+CGG - chr8_3:45055314-45055333 Msa1290320:five_prime_UTR 40.0%
!! GGCCTAATTGGCTTTGAAAA+TGG - chr8_3:45055335-45055354 Msa1290320:five_prime_UTR 40.0%
AATGGAGGTGAAAAGGTCTT+TGG - chr8_3:45055353-45055372 Msa1290320:five_prime_UTR 40.0%
ATGGAGGTGAAAAGGTCTTT+GGG - chr8_3:45055354-45055373 Msa1290320:five_prime_UTR 40.0%
AATACACAGCACAGAGAAGA+AGG + chr8_3:45055381-45055400 None:intergenic 40.0%
ATAAATATGCGCGGCCTCAT+GGG + chr8_3:45051886-45051905 None:intergenic 45.0%
TATAAATATGCGCGGCCTCA+TGG + chr8_3:45051887-45051906 None:intergenic 45.0%
GTTGTCGTCATTATTCGCTG+AGG - chr8_3:45052138-45052157 Msa1290320:three_prime_UTR 45.0%
GTCATTATTCGCTGAGGAAG+AGG - chr8_3:45052144-45052163 Msa1290320:three_prime_UTR 45.0%
ATTATTCGCTGAGGAAGAGG+AGG - chr8_3:45052147-45052166 Msa1290320:three_prime_UTR 45.0%
CTTGAGACGTATTCGAAACC+AGG + chr8_3:45052214-45052233 None:intergenic 45.0%
AGCCATTGCATGGATTCTCA+AGG - chr8_3:45052273-45052292 Msa1290320:three_prime_UTR 45.0%
CGTCGATGAATCATGGTTGA+CGG - chr8_3:45052399-45052418 Msa1290320:three_prime_UTR 45.0%
AAACAAGAGCTCACCGGTAA+AGG + chr8_3:45052578-45052597 None:intergenic 45.0%
ACACTCACGTGACATACATG+TGG + chr8_3:45053309-45053328 None:intergenic 45.0%
GTCTTTAGCAGCAAAGATGG+AGG - chr8_3:45053477-45053496 Msa1290320:intron 45.0%
TCCTTCTCTCTTGGACTTTC+AGG - chr8_3:45053510-45053529 Msa1290320:intron 45.0%
ACCTGAAAGTCCAAGAGAGA+AGG + chr8_3:45053514-45053533 None:intergenic 45.0%
ACCGAGGACGATTCTTAGAA+TGG - chr8_3:45053698-45053717 Msa1290320:intron 45.0%
CAACTGTGGCAACACTTTAG+GGG + chr8_3:45054329-45054348 None:intergenic 45.0%
GCAACTGTGGCAACACTTTA+GGG + chr8_3:45054330-45054349 None:intergenic 45.0%
! GTTGAGGTTTCTAGACAACC+TGG + chr8_3:45054559-45054578 None:intergenic 45.0%
TAACTGTATCCCCTAGAGGA+AGG - chr8_3:45054693-45054712 Msa1290320:intron 45.0%
AACTGTATCCCCTAGAGGAA+GGG - chr8_3:45054694-45054713 Msa1290320:intron 45.0%
TCCAAAATCCCCTTCCTCTA+GGG + chr8_3:45054706-45054725 None:intergenic 45.0%
TTCCAAAATCCCCTTCCTCT+AGG + chr8_3:45054707-45054726 None:intergenic 45.0%
!! AGAGAGAGAAGCATTTGACG+TGG - chr8_3:45054759-45054778 Msa1290320:intron 45.0%
! TCATAGGTGAAAGATGGTGG+GGG - chr8_3:45054821-45054840 Msa1290320:intron 45.0%
!! TGTTGAGGGTATCCCTTCAA+AGG + chr8_3:45055011-45055030 None:intergenic 45.0%
AGTACGTGTGAAGATGGCTT+TGG - chr8_3:45055083-45055102 Msa1290320:CDS 45.0%
GAGGTTTGGCCTAACTATAC+AGG - chr8_3:45055188-45055207 Msa1290320:five_prime_UTR 45.0%
TGGAGGTGAAAAGGTCTTTG+GGG - chr8_3:45055355-45055374 Msa1290320:five_prime_UTR 45.0%
!!! TTTTTATACAAAATTTTTTA+AGG - chr8_3:45053147-45053166 Msa1290320:intron 5.0%
TAAATATGCGCGGCCTCATG+GGG + chr8_3:45051885-45051904 None:intergenic 50.0%
GACAGTGGCATGAGAATCAC+CGG + chr8_3:45052007-45052026 None:intergenic 50.0%
! CACGAGTTCAAGTTCGTTCC+TGG - chr8_3:45052193-45052212 Msa1290320:three_prime_UTR 50.0%
AATGGCTTCTTCTCTGGTGC+TGG + chr8_3:45052260-45052279 None:intergenic 50.0%
CCATGCAATGGCTTCTTCTC+TGG + chr8_3:45052266-45052285 None:intergenic 50.0%
CCAGAGAAGAAGCCATTGCA+TGG - chr8_3:45052263-45052282 Msa1290320:three_prime_UTR 50.0%
! CCTTTTCATGCGCCTCGTAT+TGG + chr8_3:45052754-45052773 None:intergenic 50.0%
CCAATACGAGGCGCATGAAA+AGG - chr8_3:45052751-45052770 Msa1290320:CDS 50.0%
AGCAAAGATGGAGGAACCAC+TGG - chr8_3:45053486-45053505 Msa1290320:intron 50.0%
!! ACCACTGGTTCCTTCTCTCT+TGG - chr8_3:45053501-45053520 Msa1290320:intron 50.0%
TCCAAGAGAGAAGGAACCAG+TGG + chr8_3:45053505-45053524 None:intergenic 50.0%
GCTTGCACGCAAAGAAACTG+AGG + chr8_3:45053782-45053801 None:intergenic 50.0%
GCGTGCAAGCTAGATTCAAC+TGG - chr8_3:45053792-45053811 Msa1290320:intron 50.0%
ATCCTGAGCATGCTGAGTCA+TGG - chr8_3:45054105-45054124 Msa1290320:intron 50.0%
CATGCTGAGTCATGGTGTGA+AGG - chr8_3:45054113-45054132 Msa1290320:intron 50.0%
CGCAACTGTGGCAACACTTT+AGG + chr8_3:45054331-45054350 None:intergenic 50.0%
CACAGTTGCGAGTTACAGCT+CGG - chr8_3:45054341-45054360 Msa1290320:intron 50.0%
AAGAGGAAATAAGGTGGGCC+AGG - chr8_3:45054538-45054557 Msa1290320:intron 50.0%
GGTGTAACTGTATCCCCTAG+AGG - chr8_3:45054689-45054708 Msa1290320:intron 50.0%
ACTGTATCCCCTAGAGGAAG+GGG - chr8_3:45054695-45054714 Msa1290320:intron 50.0%
CCAAAATCCCCTTCCTCTAG+GGG + chr8_3:45054705-45054724 None:intergenic 50.0%
TTCATAGTGGCCGGCCTAAT+TGG - chr8_3:45055323-45055342 Msa1290320:five_prime_UTR 50.0%
CTGTGGGCACACTGGACATA+CGG + chr8_3:45051758-45051777 None:intergenic 55.0%
! CTTTTGTGCTGTGGGCACAC+TGG + chr8_3:45051766-45051785 None:intergenic 55.0%
AGTCCTCACCGGAGATTCAC+CGG - chr8_3:45052072-45052091 Msa1290320:three_prime_UTR 55.0%
ATTCCGGTGAATCTCCGGTG+AGG + chr8_3:45052078-45052097 None:intergenic 55.0%
GCAGCATTCCGGTGAATCTC+CGG + chr8_3:45052083-45052102 None:intergenic 55.0%
TGGCTTCGAGAGAGCGAGAT+TGG + chr8_3:45052240-45052259 None:intergenic 55.0%
! ACGGAGAGGTACGCCGTTAA+CGG + chr8_3:45052524-45052543 None:intergenic 55.0%
GCAGCTGCAGCAATGCATGA+TGG + chr8_3:45054041-45054060 None:intergenic 55.0%
CACCATGACTCAGCATGCTC+AGG + chr8_3:45054110-45054129 None:intergenic 55.0%
CGAGCTGTAACTCGCAACTG+TGG + chr8_3:45054343-45054362 None:intergenic 55.0%
GAGTTACAGCTCGGACCAGA+AGG - chr8_3:45054350-45054369 Msa1290320:intron 55.0%
TTACAGCTCGGACCAGAAGG+TGG - chr8_3:45054353-45054372 Msa1290320:intron 55.0%
! TGATGACGACGACGAGACAC+TGG + chr8_3:45054380-45054399 None:intergenic 55.0%
GAGGGTGAAGAGGAGGAAGA+TGG + chr8_3:45054406-45054425 None:intergenic 55.0%
CAACGGGAAAGAGGGTGAAG+AGG + chr8_3:45054416-45054435 None:intergenic 55.0%
! CCCCTAGAGGAAGGGGATTT+TGG - chr8_3:45054702-45054721 Msa1290320:intron 55.0%
! TTGGTGTCCAACGCGTGTGA+TGG - chr8_3:45055221-45055240 Msa1290320:five_prime_UTR 55.0%
TGGTGTCCAACGCGTGTGAT+GGG - chr8_3:45055222-45055241 Msa1290320:five_prime_UTR 55.0%
GTGTTTCCCATCACACGCGT+TGG + chr8_3:45055231-45055250 None:intergenic 55.0%
!! CAGTCGGAGGTGCTTGACAG+TGG + chr8_3:45052022-45052041 None:intergenic 60.0%
GACTCGTCGGAAGTCCTCAC+CGG - chr8_3:45052061-45052080 Msa1290320:three_prime_UTR 60.0%
TCGAGGTCGGAGCAGCATTC+CGG + chr8_3:45052094-45052113 None:intergenic 60.0%
CGAGACACTGGACCACCTTC+TGG + chr8_3:45054368-45054387 None:intergenic 60.0%
CGGGAAAGAGGGTGAAGAGG+AGG + chr8_3:45054413-45054432 None:intergenic 60.0%
!! GTGAAAGATGGTGGGGGCAG+TGG - chr8_3:45054827-45054846 Msa1290320:intron 60.0%
TCCGACTGCGAGCTCCTCTG+CGG - chr8_3:45052034-45052053 Msa1290320:three_prime_UTR 65.0%
CCTCTGCGGCGAAGACTCGT+CGG - chr8_3:45052048-45052067 Msa1290320:three_prime_UTR 65.0%
GCAGAGGAGCTCGCAGTCGG+AGG + chr8_3:45052035-45052054 None:intergenic 70.0%
GCCGCAGAGGAGCTCGCAGT+CGG + chr8_3:45052038-45052057 None:intergenic 70.0%
CCGACGAGTCTTCGCCGCAG+AGG + chr8_3:45052051-45052070 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 45051632 45055512 45051632 ID=Msa1290320;Name=Msa1290320
chr8_3 mRNA 45051632 45055512 45051632 ID=Msa1290320-mRNA-1;Parent=Msa1290320;Name=Msa1290320-mRNA-1;_AED=0.04;_eAED=0.04;_QI=354|1|1|1|1|1|6|1011|355
chr8_3 exon 45051632 45052882 45051632 ID=Msa1290320-mRNA-1:exon:12427;Parent=Msa1290320-mRNA-1
chr8_3 exon 45052999 45053129 45052999 ID=Msa1290320-mRNA-1:exon:12426;Parent=Msa1290320-mRNA-1
chr8_3 exon 45053271 45053472 45053271 ID=Msa1290320-mRNA-1:exon:12425;Parent=Msa1290320-mRNA-1
chr8_3 exon 45053613 45053711 45053613 ID=Msa1290320-mRNA-1:exon:12424;Parent=Msa1290320-mRNA-1
chr8_3 exon 45054561 45054647 45054561 ID=Msa1290320-mRNA-1:exon:12423;Parent=Msa1290320-mRNA-1
chr8_3 exon 45054850 45055512 45054850 ID=Msa1290320-mRNA-1:exon:12422;Parent=Msa1290320-mRNA-1
chr8_3 five_prime_UTR 45055159 45055512 45055159 ID=Msa1290320-mRNA-1:five_prime_utr;Parent=Msa1290320-mRNA-1
chr8_3 CDS 45054850 45055158 45054850 ID=Msa1290320-mRNA-1:cds;Parent=Msa1290320-mRNA-1
chr8_3 CDS 45054561 45054647 45054561 ID=Msa1290320-mRNA-1:cds;Parent=Msa1290320-mRNA-1
chr8_3 CDS 45053613 45053711 45053613 ID=Msa1290320-mRNA-1:cds;Parent=Msa1290320-mRNA-1
chr8_3 CDS 45053271 45053472 45053271 ID=Msa1290320-mRNA-1:cds;Parent=Msa1290320-mRNA-1
chr8_3 CDS 45052999 45053129 45052999 ID=Msa1290320-mRNA-1:cds;Parent=Msa1290320-mRNA-1
chr8_3 CDS 45052643 45052882 45052643 ID=Msa1290320-mRNA-1:cds;Parent=Msa1290320-mRNA-1
chr8_3 three_prime_UTR 45051632 45052642 45051632 ID=Msa1290320-mRNA-1:three_prime_utr;Parent=Msa1290320-mRNA-1
Gene Sequence

>Msa1290320

ATGAATGACGAGCAATTACCGGTGATTCTCATGCCACTGTCAAGCACCTCCGACTGCGAGCTCCTCTGCGGCGAAGACTCGTCGGAAGTCCTCACCGGAGATTCACCGGAATGCTGCTCCGACCTCGATTCATCATCATCATCACCGTTGCCGTTGTCGTCATTATTCGCTGAGGAAGAGGAGGAGTCGATTGCTGTTTTCATCGAGCACGAGTTCAAGTTCGTTCCTGGTTTCGAATACGTCTCAAGATTCCAATCTCGCTCTCTCGAAGCCAGCACCAGAGAAGAAGCCATTGCATGGATTCTCAAGGTACATGAGTATTATGGATTTCAGCCGTTAACGGCGTACCTCTCCGTTAACTATATGGATCGGTTTTTGGATTCTCGACCTTTACCGGAATCAAATGGATGGCCACTGCAACTTTTATCTGTTGCATGTTTGTCTTTAGCAGCAAAGATGGAGGAACCACTGGTTCCTTCTCTCTTGGACTTTCAGGTTGAAGGTGCCAAATACATATTTCAACCGAGGACGATTCTTAGAATGGAGCTGCTTGTTCTGACTATTTTGGATTGGAGGCTAAGATCAGTCACCCCACTTAGTTTCCTCAGTTTCTTTGCGTGCAAGCTAGATTCAACTGGAACTTTCACTCACTTCCTCATTTCACGTGCTACAGAAATCATCTTATCTAATATCCAAGATGCTAGCTTTCTTGCTTACAGGCCATCATGCATTGCTGCAGCTGCCATACTCTCTGCAGCTAATGAAATTCCTAATTGGTCTTTTGTTAATCCTGAGCATGCTGAGTCATGGTGTGAAGGACTAAGCAAAGAAAAAATTATAGGGTGCTATGAGTTGATGCAAGAAATTGTGAGTAGCAATAACCAAAGAAATGCCCCTAAAGTGTTGCCACAGTTGCGAGTTACAGCTCGGACCAGAAGGTGGTCCAGTGTCTCGTCGTCGTCATCATCTCCATCTTCCTCCTCTTCACCCTCTTTCCCGTTGTCGTATAAAAAGAGGAAATTAAATAGCTGTTTCTGGGTAGATGATGACAAAGGAAACTTAGAGTGA
Protein sequence

>Msa1290320

MNDEQLPVILMPLSSTSDCELLCGEDSSEVLTGDSPECCSDLDSSSSSPLPLSSLFAEEEEESIAVFIEHEFKFVPGFEYVSRFQSRSLEASTREEAIAWILKVHEYYGFQPLTAYLSVNYMDRFLDSRPLPESNGWPLQLLSVACLSLAAKMEEPLVPSLLDFQVEGAKYIFQPRTILRMELLVLTILDWRLRSVTPLSFLSFFACKLDSTGTFTHFLISRATEIILSNIQDASFLAYRPSCIAAAAILSAANEIPNWSFVNPEHAESWCEGLSKEKIIGCYELMQEIVSSNNQRNAPKVLPQLRVTARTRRWSSVSSSSSSPSSSSSPSFPLSYKKRKLNSCFWVDDDKGNLE*