AlfalfaGEDB | Msa1297350

M. sativa cultivar zhongmu-4 / Msa1297350

KEGG: -
GO: -
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1297350	XP_003608592.1	83.438	320	45	3	1	319	16	328	0.0	539

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1297350	sp\|Q1PF16\|BH019_ARATH	50.556	180	81	5	139	311	112	290	1.08e-52	177

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1297350	G7JGD6	83.438	320	45	3	1	319	16	328	0.0	539

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TFs/TRs:

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Gene ID	Type	Classification
Msa1297350	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1133370	Msa1297350	0.931792	2.234692e-94	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1297350	MtrunA17_Chr4g0054561	83.438	320	45	3	1	319	16	328	0.0	539
Msa1297350	MtrunA17_Chr4g0054551	81.875	320	50	3	1	319	16	328	0.0	533
Msa1297350	MtrunA17_Chr5g0400671	44.023	343	129	11	1	315	15	322	1.48e-75	234
Msa1297350	MtrunA17_Chr4g0049331	49.091	220	103	5	105	319	95	310	7.58e-57	186
Msa1297350	MtrunA17_Chr2g0280161	49.495	198	88	4	118	311	156	345	4.08e-52	175
Msa1297350	MtrunA17_Chr5g0400661	44.635	233	103	8	105	315	105	333	7.25e-51	171
Msa1297350	MtrunA17_Chr8g0336681	44.149	188	92	4	139	315	149	334	1.22e-47	163
Msa1297350	MtrunA17_Chr6g0468491	47.674	172	86	3	143	311	134	304	2.01e-47	162
Msa1297350	MtrunA17_Chr5g0400621	44.167	240	127	4	77	311	87	324	1.39e-46	160
Msa1297350	MtrunA17_Chr2g0333621	46.354	192	96	3	129	315	121	310	1.66e-46	159

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1297350	AT2G22760.1	50.556	180	81	5	139	311	112	290	1.10e-53	177
Msa1297350	AT4G37850.2	43.426	251	114	7	88	319	87	328	1.90e-52	176
Msa1297350	AT4G37850.1	43.426	251	114	7	88	319	87	328	1.90e-52	176
Msa1297350	AT2G22760.2	50.610	164	74	4	139	296	112	274	2.40e-48	164
Msa1297350	AT2G22770.1	47.059	221	95	6	116	315	99	318	6.75e-48	163
Msa1297350	AT2G22750.2	43.825	251	108	9	89	318	66	304	3.03e-47	161
Msa1297350	AT2G22750.3	43.825	251	108	9	89	318	94	332	3.75e-47	162
Msa1297350	AT2G22750.5	43.825	251	108	9	89	318	90	328	4.25e-47	162
Msa1297350	AT2G22750.4	43.825	251	107	10	89	318	94	331	9.72e-46	158
Msa1297350	AT2G22750.1	43.825	251	107	10	89	318	66	303	1.07e-45	157

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Find 48 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACATATGAAGCAACTAC+AGG	0.565504	8_3:-57562127	Msa1297350:CDS
AAAATTGAAGCAAGGGTTTC+TGG	0.125232	8_3:-57561973	Msa1297350:CDS
AAGAAGCCTAAAACCGAGAT+TGG	0.405458	8_3:-57562678	Msa1297350:CDS
AAGAATAAGTACTCTCTTGT+GGG	0.460152	8_3:+57562889	None:intergenic
AATAAATAGAAACCCTATTC+AGG	0.429952	8_3:-57562580	Msa1297350:CDS
ACAGGAAAGAGTGAAGACAT+TGG	0.652927	8_3:-57562109	Msa1297350:CDS
ACATGATCTTGAGCCTGAAT+AGG	0.366395	8_3:+57562567	None:intergenic
ACTTTGCCTGCAGATGGACA+AGG	0.625234	8_3:-57562172	Msa1297350:intron
AGAAGCCTAAAACCGAGATT+GGG	0.552154	8_3:-57562677	Msa1297350:CDS
AGAATAAGTACTCTCTTGTG+GGG	0.633544	8_3:+57562890	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAATGATCATGAATACCCAA+TGG	-	chr8_3:57561390-57561409	Msa1297350:CDS	30.0%
AAGAATAAGTACTCTCTTGT+GGG	+	chr8_3:57561471-57561490	None:intergenic	30.0%
AATCTCACACAATTTGATTG+TGG	-	chr8_3:57561969-57561988	Msa1297350:CDS	30.0%
ATATTGCATGTTATATGCAG+GGG	+	chr8_3:57562025-57562044	None:intergenic	30.0%
CATATTGCATGTTATATGCA+GGG	+	chr8_3:57562026-57562045	None:intergenic	30.0%
AGTTAGTATAGTTGCAATTG+AGG	-	chr8_3:57562120-57562139	Msa1297350:CDS	30.0%
AAAACATATGAAGCAACTAC+AGG	-	chr8_3:57562230-57562249	Msa1297350:intron	30.0%
TGATATAACCATTGTTACTC+AGG	-	chr8_3:57562539-57562558	Msa1297350:CDS	30.0%
ACTTGTCTTAATTGCATGTT+TGG	-	chr8_3:57562592-57562611	Msa1297350:CDS	30.0%
AACTAGGCTAGAACATAAAA+TGG	+	chr8_3:57562749-57562768	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	57561370	57563009	57561370	ID=Msa1297350;Name=Msa1297350
chr8_3	mRNA	57561370	57563009	57561370	ID=Msa1297350-mRNA-1;Parent=Msa1297350;Name=Msa1297350-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0\|0.5\|0\|1\|1\|1\|3\|0\|319
chr8_3	exon	57562488	57563009	57562488	ID=Msa1297350-mRNA-1:exon:16509;Parent=Msa1297350-mRNA-1
chr8_3	exon	57561819	57562181	57561819	ID=Msa1297350-mRNA-1:exon:16508;Parent=Msa1297350-mRNA-1
chr8_3	exon	57561370	57561444	57561370	ID=Msa1297350-mRNA-1:exon:16507;Parent=Msa1297350-mRNA-1
chr8_3	CDS	57562488	57563009	57562488	ID=Msa1297350-mRNA-1:cds;Parent=Msa1297350-mRNA-1
chr8_3	CDS	57561819	57562181	57561819	ID=Msa1297350-mRNA-1:cds;Parent=Msa1297350-mRNA-1
chr8_3	CDS	57561370	57561444	57561370	ID=Msa1297350-mRNA-1:cds;Parent=Msa1297350-mRNA-1

Gene Sequence

>Msa1297350

ATGGAGGACACTACTTTGTTTAATGATCATGAATACCCAATGGACTCATATGCATTCGAGTTTGATGATATGGCATACTTCAAGTCTTTCTCAGCATCCCCACAAGAGAGTACTTATTCTTCTCATAACAACAACAGCAGCAAGCGAATCCATTCCGAAAGCACACAGAACAATTCTTTCCCCATTGAAAGTAGTCCTGATCAATCTGTTGCTTCACCTACACCAACAAAACTAAGACTCAAGTCTTCTTCATCTTCATCTTCATCTTCCAAGATCATTTCTTTTGAACCCAACATGGATACAAGGGTGAAGAAGCCTAAAACCGAGATTGGGTATGGAGAAAACTTGAATTTTGGAAGTGTGATATCTCAAGGTGATTATTATAAACGAGAGAACAAGGTTGCTGCAATAAATAGAAACCCTATTCAGGCTCAAGATCATGTTATTGCCGAAAGAAAACGAAGGGAAAAGCTTAACCAGAGATTCATTGCTCTTTCATCTCTTCTTCCTGGACTCAAAAAGATGGACAAGGCAACTGTTTTGGAAGATGCTATAAAACATATGAAGCAACTACAGGAAAGAGTGAAGACATTGGAGGAACAAGTAGCCGATAAAAAAGTCGAATCAGCAGTTTTTGTGAAGAGATCTATTCTCTTCGACGAAGATGAAAGCTCCTCTTGCGACAAAAATTCAGACCAATCACTCCCAAAAATTGAAGCAAGGGTTTCTGGAAAAGACATGCTCATCAGAATCCATTGCGACAGATATTGTGGCCGATCTGCGACCGCAATACTCAACGAATTAGAAAAGCTTCGCCTCACTGTTCAGAGCACTTCTATTTTGCCATTTGGAAATAACTATCTTGATATAACCATTGTTACTCAGATGAATAAAGAATATTGCTTGACAATAAAGGATCTCGTAAGGAGCATAAGTCAGGTTTTGAGGCAGCTCATTTGA

Protein sequence

>Msa1297350

MEDTTLFNDHEYPMDSYAFEFDDMAYFKSFSASPQESTYSSHNNNSSKRIHSESTQNNSFPIESSPDQSVASPTPTKLRLKSSSSSSSSSKIISFEPNMDTRVKKPKTEIGYGENLNFGSVISQGDYYKRENKVAAINRNPIQAQDHVIAERKRREKLNQRFIALSSLLPGLKKMDKATVLEDAIKHMKQLQERVKTLEEQVADKKVESAVFVKRSILFDEDESSSCDKNSDQSLPKIEARVSGKDMLIRIHCDRYCGRSATAILNELEKLRLTVQSTSILPFGNNYLDITIVTQMNKEYCLTIKDLVRSISQVLRQLI*