Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1297760 | sp|Q9LVZ7|PDCT1_ARATH | 77.686 | 121 | 27 | 0 | 3 | 123 | 160 | 280 | 9.85e-63 | 196 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1297760 | G7JGX5 | 90.769 | 130 | 12 | 0 | 3 | 132 | 138 | 267 | 1.37e-79 | 245 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1172430 | Msa1297760 | 0.825442 | 4.678164e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1297760 | MtrunA17_Chr4g0003201 | 90.769 | 130 | 12 | 0 | 3 | 132 | 138 | 267 | 2.64e-83 | 245 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1297760 | AT3G15820.1 | 77.686 | 121 | 27 | 0 | 3 | 123 | 160 | 280 | 1.00e-63 | 196 |
| Msa1297760 | AT3G15830.1 | 73.387 | 124 | 33 | 0 | 3 | 126 | 155 | 278 | 1.29e-62 | 193 |
Find 28 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATGTTGAATAGGGATTCT+TGG | 0.322192 | 8_3:-58302970 | Msa1297760:intron |
| AGGCTATTACACTATTGATT+TGG | 0.328688 | 8_3:-58302775 | Msa1297760:CDS |
| TACACTATTGATTTGGCTGT+TGG | 0.339883 | 8_3:-58302768 | Msa1297760:CDS |
| TTCAGGTCATGTGGCGGGTT+CGG | 0.347386 | 8_3:-58302898 | Msa1297760:CDS |
| TTATAGACTGGTTTAATTGA+TGG | 0.356357 | 8_3:-58303677 | Msa1297760:intron |
| AATGTTGAATAGGGATTCTT+GGG | 0.361886 | 8_3:-58302969 | Msa1297760:intron |
| ATTGATTTGGCTGTTGGGTT+AGG | 0.379250 | 8_3:-58302762 | Msa1297760:CDS |
| TTGGCTGTTGGGTTAGGTGC+TGG | 0.405118 | 8_3:-58302756 | Msa1297760:CDS |
| TATATATTTGACTCTTTAGC+TGG | 0.413885 | 8_3:-58302732 | Msa1297760:CDS |
| ACACTATTGATTTGGCTGTT+GGG | 0.428713 | 8_3:-58302767 | Msa1297760:CDS |
| CTCGTGTGGACTTTCCAGTT+GGG | 0.452431 | 8_3:-58302944 | Msa1297760:CDS |
| ACATGAGGAGGATGAAGAGA+TGG | 0.456825 | 8_3:-58302860 | Msa1297760:CDS |
| TTTACATGTCGTGGGATTTG+TGG | 0.464311 | 8_3:-58303620 | Msa1297760:CDS |
| CATGAGGAGGATGAAGAGAT+GGG | 0.476047 | 8_3:-58302859 | Msa1297760:CDS |
| GAACGAGTCACCTGAGGCAA+TGG | 0.476165 | 8_3:+58303581 | None:intergenic |
| GGGTTCGGTGATAGCTTCAT+TGG | 0.496149 | 8_3:-58302883 | Msa1297760:CDS |
| TCTCGTGTGGACTTTCCAGT+TGG | 0.496967 | 8_3:-58302945 | Msa1297760:CDS |
| TTACATGTCGTGGGATTTGT+GGG | 0.512699 | 8_3:-58303619 | Msa1297760:CDS |
| GAGGATGAAGAGATGGGAGA+TGG | 0.513956 | 8_3:-58302853 | Msa1297760:CDS |
| CACACAGCTCCCATTGCCTC+AGG | 0.519834 | 8_3:-58303591 | Msa1297760:intron |
| AACGAGTCACCTGAGGCAAT+GGG | 0.519909 | 8_3:+58303582 | None:intergenic |
| GGGATTCTTGGGTTCTCGTG+TGG | 0.527473 | 8_3:-58302958 | Msa1297760:CDS |
| TGAAGAGATGGGAGATGGCA+TGG | 0.534871 | 8_3:-58302848 | Msa1297760:CDS |
| TTGCAAGCTGTAAGATTGCT+TGG | 0.542082 | 8_3:-58302804 | Msa1297760:CDS |
| GTAAGATTGCTTGGTACAAG+AGG | 0.580563 | 8_3:-58302795 | Msa1297760:CDS |
| TGATAGCTTCATTGGACATG+AGG | 0.619875 | 8_3:-58302875 | Msa1297760:CDS |
| TAGCTTCATTGGACATGAGG+AGG | 0.656474 | 8_3:-58302872 | Msa1297760:CDS |
| CTGAGGCAATGGGAGCTGTG+TGG | 0.711136 | 8_3:+58303592 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTAATGTTTATTAAAAATT+GGG | + | chr8_3:58302873-58302892 | None:intergenic | 10.0% |
| !! | TGTTAATGTTTATTAAAAAT+TGG | + | chr8_3:58302874-58302893 | None:intergenic | 10.0% |
| !!! | TTTATCTGAATTGATTTTTT+TGG | - | chr8_3:58303177-58303196 | Msa1297760:intron | 15.0% |
| !! | ATTTGAATTCAATTAACATT+GGG | - | chr8_3:58304075-58304094 | Msa1297760:intron | 15.0% |
| !! | AATGTTTATTAAAAATTGGG+TGG | + | chr8_3:58302870-58302889 | None:intergenic | 20.0% |
| !!! | TGTTAAAATTTGTGTTTTGT+GGG | - | chr8_3:58303048-58303067 | Msa1297760:intron | 20.0% |
| !!! | AAATTTTATGTTTTGTGTGT+AGG | - | chr8_3:58303246-58303265 | Msa1297760:intron | 20.0% |
| !!! | AGAGTTGAAATTATTGTTTT+AGG | + | chr8_3:58303522-58303541 | None:intergenic | 20.0% |
| !!! | AAAAAATGAAGCATTTTCTT+TGG | + | chr8_3:58303643-58303662 | None:intergenic | 20.0% |
| !!! | ATAGAAGTGATTCTAAAAAA+TGG | - | chr8_3:58303793-58303812 | Msa1297760:intron | 20.0% |
| !!! | TAGAAGTGATTCTAAAAAAT+GGG | - | chr8_3:58303794-58303813 | Msa1297760:intron | 20.0% |
| !!! | GAATTCAAACATAGTTTTTT+TGG | + | chr8_3:58303849-58303868 | None:intergenic | 20.0% |
| !! | GATTTGAATTCAATTAACAT+TGG | - | chr8_3:58304074-58304093 | Msa1297760:intron | 20.0% |
| !! | TTTGAATTCAATTAACATTG+GGG | - | chr8_3:58304076-58304095 | Msa1297760:intron | 20.0% |
| !! | ATTAACAAAACAAAAACACA+AGG | + | chr8_3:58304183-58304202 | None:intergenic | 20.0% |
| !!! | TTGTTTTGTTAATGTTGAAT+AGG | - | chr8_3:58304190-58304209 | Msa1297760:intron | 20.0% |
| !!! | TGTTTTGTTAATGTTGAATA+GGG | - | chr8_3:58304191-58304210 | Msa1297760:intron | 20.0% |
| !! | GTGTTAAAATTTGTGTTTTG+TGG | - | chr8_3:58303047-58303066 | Msa1297760:intron | 25.0% |
| ! | AGATACATTCACATAAAAGT+GGG | + | chr8_3:58303121-58303140 | None:intergenic | 25.0% |
| ! | TAGATACATTCACATAAAAG+TGG | + | chr8_3:58303122-58303141 | None:intergenic | 25.0% |
| ! | AGATACATTCACATAAAAGT+GGG | + | chr8_3:58303121-58303140 | None:intergenic | 25.0% |
| ! | TAGATACATTCACATAAAAG+TGG | + | chr8_3:58303122-58303141 | None:intergenic | 25.0% |
| !! | AAAATTTGTGTTTTGTGTGT+AGG | - | chr8_3:58303440-58303459 | Msa1297760:intron | 25.0% |
| ! | ACGAGTTATATATTATAGAC+TGG | - | chr8_3:58303480-58303499 | Msa1297760:intron | 25.0% |
| !!! | TTATAGACTGGTTTAATTGA+TGG | - | chr8_3:58303492-58303511 | Msa1297760:intron | 25.0% |
| !!! | AAACATGCATTTAGTTCTAT+TGG | + | chr8_3:58303754-58303773 | None:intergenic | 25.0% |
| !!! | CAAACATAGTTTTTTTGGAT+TGG | + | chr8_3:58303844-58303863 | None:intergenic | 25.0% |
| ! | TTTAACATGAAATCGAAGTT+TGG | - | chr8_3:58303953-58303972 | Msa1297760:intron | 25.0% |
| !!! | TCATTTTTCCTGTTTTTTTC+AGG | - | chr8_3:58304254-58304273 | Msa1297760:intron | 25.0% |
| ! | TATATATTTGACTCTTTAGC+TGG | - | chr8_3:58304437-58304456 | Msa1297760:intron | 25.0% |
| ! | TGTTGATAAGTCAGTTACTT+GGG | + | chr8_3:58302714-58302733 | None:intergenic | 30.0% |
| AAAATAATCATCCCTTGATG+AGG | - | chr8_3:58302785-58302804 | Msa1297760:CDS | 30.0% | |
| AGAAAATAACACCTCATCAA+GGG | + | chr8_3:58302799-58302818 | None:intergenic | 30.0% | |
| ATTAAAAATTGGGTGGTAGT+GGG | + | chr8_3:58302863-58302882 | None:intergenic | 30.0% | |
| TATTAAAAATTGGGTGGTAG+TGG | + | chr8_3:58302864-58302883 | None:intergenic | 30.0% | |
| !!! | GTTTTGTTTGCATTGTTAGT+TGG | - | chr8_3:58303025-58303044 | Msa1297760:intron | 30.0% |
| ! | AAAATTTGTGTTTTGTGGGT+AGG | - | chr8_3:58303052-58303071 | Msa1297760:intron | 30.0% |
| !!! | GTTTTGTTTGCAATGTTAGT+TGG | - | chr8_3:58303219-58303238 | Msa1297760:intron | 30.0% |
| !!! | TTTCTCTCAGTCGATTTTTT+TGG | - | chr8_3:58303371-58303390 | Msa1297760:intron | 30.0% |
| !!! | GTTTTGTTTGCATTGTTAGT+TGG | - | chr8_3:58303025-58303044 | Msa1297760:intron | 30.0% |
| ! | TTTTCATGTTTACATGTCGT+GGG | - | chr8_3:58303541-58303560 | Msa1297760:intron | 30.0% |
| !! | TGCATTTAGTTCTATTGGAT+TGG | + | chr8_3:58303749-58303768 | None:intergenic | 30.0% |
| CATTGTAAGCTTCTAGTAAT+GGG | + | chr8_3:58303896-58303915 | None:intergenic | 30.0% | |
| TCATTGTAAGCTTCTAGTAA+TGG | + | chr8_3:58303897-58303916 | None:intergenic | 30.0% | |
| TACTAGAAGCTTACAATGAA+AGG | - | chr8_3:58303898-58303917 | Msa1297760:intron | 30.0% | |
| ACTAGAAGCTTACAATGAAA+GGG | - | chr8_3:58303899-58303918 | Msa1297760:intron | 30.0% | |
| TAATGTTGAATAGGGATTCT+TGG | - | chr8_3:58304199-58304218 | Msa1297760:intron | 30.0% | |
| AATGTTGAATAGGGATTCTT+GGG | - | chr8_3:58304200-58304219 | Msa1297760:intron | 30.0% | |
| AAAAATGATACATTCCCAAC+TGG | + | chr8_3:58304242-58304261 | None:intergenic | 30.0% | |
| ! | AGGCTATTACACTATTGATT+TGG | - | chr8_3:58304394-58304413 | Msa1297760:intron | 30.0% |
| ! | CTGTTGATAAGTCAGTTACT+TGG | + | chr8_3:58302715-58302734 | None:intergenic | 35.0% |
| GAGAAAATAACACCTCATCA+AGG | + | chr8_3:58302800-58302819 | None:intergenic | 35.0% | |
| !! | ACGACAATACGTGTTTTGTT+TGG | - | chr8_3:58303143-58303162 | Msa1297760:intron | 35.0% |
| !! | ACGACAATACGTGTTTTGTT+TGG | - | chr8_3:58303143-58303162 | Msa1297760:intron | 35.0% |
| ! | CTTTTCATGTTTACATGTCG+TGG | - | chr8_3:58303540-58303559 | Msa1297760:intron | 35.0% |
| ! | ATTCGTCCTAAGAGCAATTT+TGG | - | chr8_3:58303711-58303730 | Msa1297760:intron | 35.0% |
| TAACCTCCAAAATTGCTCTT+AGG | + | chr8_3:58303720-58303739 | None:intergenic | 35.0% | |
| ACAATGAAAGGGATTCACTT+CGG | - | chr8_3:58303910-58303929 | Msa1297760:intron | 35.0% | |
| GTATCTGTCTCTCAAATTAG+TGG | + | chr8_3:58304038-58304057 | None:intergenic | 35.0% | |
| !! | TACACTATTGATTTGGCTGT+TGG | - | chr8_3:58304401-58304420 | Msa1297760:intron | 35.0% |
| !! | ACACTATTGATTTGGCTGTT+GGG | - | chr8_3:58304402-58304421 | Msa1297760:intron | 35.0% |
| TTTACATGTCGTGGGATTTG+TGG | - | chr8_3:58303549-58303568 | Msa1297760:intron | 40.0% | |
| TTACATGTCGTGGGATTTGT+GGG | - | chr8_3:58303550-58303569 | Msa1297760:intron | 40.0% | |
| !!! | TTTTGGATTGGTCAGTGTGT+AGG | + | chr8_3:58303832-58303851 | None:intergenic | 40.0% |
| GTCTCTCAAATTAGTGGCAT+AGG | + | chr8_3:58304032-58304051 | None:intergenic | 40.0% | |
| CCACATGACCTGAAAAAAAC+AGG | + | chr8_3:58304265-58304284 | None:intergenic | 40.0% | |
| !!! | CCTGTTTTTTTCAGGTCATG+TGG | - | chr8_3:58304262-58304281 | Msa1297760:intron | 40.0% |
| !!! | GTTTTTTTCAGGTCATGTGG+CGG | - | chr8_3:58304265-58304284 | Msa1297760:intron | 40.0% |
| !!! | TTTTTTTCAGGTCATGTGGC+GGG | - | chr8_3:58304266-58304285 | Msa1297760:intron | 40.0% |
| TGATAGCTTCATTGGACATG+AGG | - | chr8_3:58304294-58304313 | Msa1297760:intron | 40.0% | |
| TTGCAAGCTGTAAGATTGCT+TGG | - | chr8_3:58304365-58304384 | Msa1297760:intron | 40.0% | |
| GTAAGATTGCTTGGTACAAG+AGG | - | chr8_3:58304374-58304393 | Msa1297760:intron | 40.0% | |
| !! | ATTGATTTGGCTGTTGGGTT+AGG | - | chr8_3:58304407-58304426 | Msa1297760:intron | 40.0% |
| TGAAATGAACGAGTCACCTG+AGG | + | chr8_3:58303597-58303616 | None:intergenic | 45.0% | |
| ! | CGTCCTAAGAGCAATTTTGG+AGG | - | chr8_3:58303714-58303733 | Msa1297760:intron | 45.0% |
| TAGCTTCATTGGACATGAGG+AGG | - | chr8_3:58304297-58304316 | Msa1297760:intron | 45.0% | |
| ACATGAGGAGGATGAAGAGA+TGG | - | chr8_3:58304309-58304328 | Msa1297760:intron | 45.0% | |
| CATGAGGAGGATGAAGAGAT+GGG | - | chr8_3:58304310-58304329 | Msa1297760:intron | 45.0% | |
| AACGAGTCACCTGAGGCAAT+GGG | + | chr8_3:58303590-58303609 | None:intergenic | 50.0% | |
| TCTCGTGTGGACTTTCCAGT+TGG | - | chr8_3:58304224-58304243 | Msa1297760:intron | 50.0% | |
| CTCGTGTGGACTTTCCAGTT+GGG | - | chr8_3:58304225-58304244 | Msa1297760:intron | 50.0% | |
| !! | GGGTTCGGTGATAGCTTCAT+TGG | - | chr8_3:58304286-58304305 | Msa1297760:intron | 50.0% |
| GAGGATGAAGAGATGGGAGA+TGG | - | chr8_3:58304316-58304335 | Msa1297760:intron | 50.0% | |
| TGAAGAGATGGGAGATGGCA+TGG | - | chr8_3:58304321-58304340 | Msa1297760:intron | 50.0% | |
| GAACGAGTCACCTGAGGCAA+TGG | + | chr8_3:58303591-58303610 | None:intergenic | 55.0% | |
| GGGATTCTTGGGTTCTCGTG+TGG | - | chr8_3:58304211-58304230 | Msa1297760:intron | 55.0% | |
| !! | TTCAGGTCATGTGGCGGGTT+CGG | - | chr8_3:58304271-58304290 | Msa1297760:intron | 55.0% |
| ! | TTGGCTGTTGGGTTAGGTGC+TGG | - | chr8_3:58304413-58304432 | Msa1297760:intron | 55.0% |
| CTGAGGCAATGGGAGCTGTG+TGG | + | chr8_3:58303580-58303599 | None:intergenic | 60.0% | |
| CACACAGCTCCCATTGCCTC+AGG | - | chr8_3:58303578-58303597 | Msa1297760:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_3 | gene | 58302689 | 58304502 | 58302689 | ID=Msa1297760;Name=Msa1297760 |
| chr8_3 | mRNA | 58302689 | 58304502 | 58302689 | ID=Msa1297760-mRNA-1;Parent=Msa1297760;Name=Msa1297760-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0|0|0.66|0|0|3|0|132 |
| chr8_3 | exon | 58304497 | 58304502 | 58304497 | ID=Msa1297760-mRNA-1:exon:16753;Parent=Msa1297760-mRNA-1 |
| chr8_3 | exon | 58303592 | 58303693 | 58303592 | ID=Msa1297760-mRNA-1:exon:16752;Parent=Msa1297760-mRNA-1 |
| chr8_3 | exon | 58302689 | 58302979 | 58302689 | ID=Msa1297760-mRNA-1:exon:16751;Parent=Msa1297760-mRNA-1 |
| chr8_3 | CDS | 58304497 | 58304502 | 58304497 | ID=Msa1297760-mRNA-1:cds;Parent=Msa1297760-mRNA-1 |
| chr8_3 | CDS | 58303592 | 58303693 | 58303592 | ID=Msa1297760-mRNA-1:cds;Parent=Msa1297760-mRNA-1 |
| chr8_3 | CDS | 58302689 | 58302979 | 58302689 | ID=Msa1297760-mRNA-1:cds;Parent=Msa1297760-mRNA-1 |
| Gene Sequence |
| Protein sequence |
