AlfalfaGEDB | Msa1300180

M. sativa cultivar zhongmu-4 / Msa1300180

KEGG: -
GO: -
PFAM: PF03110.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1300180	sp\|Q94JW8\|SPL6_ARATH	64.286	126	32	3	75	197	83	198	9.43e-44	161

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1300180	A0A072V1P8	96.170	470	18	0	1	470	1	470	0.0	936

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TFs/TRs:

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Gene ID	Type	Classification
Msa1300180	TF	SBP

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0392490	Msa1300180	0.801164	1.024712e-48	-8.615850e-47
Msa0487630	Msa1300180	0.804580	2.017439e-49	-8.615850e-47
Msa1213110	Msa1300180	0.916706	1.290043e-85	-8.615850e-47
Msa1213220	Msa1300180	0.932351	9.703005e-95	-8.615850e-47
Msa1260370	Msa1300180	0.852041	5.987449e-61	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1300180	MtrunA17_Chr4g0061651	96.170	470	18	0	1	470	1	470	0.0	936
Msa1300180	MtrunA17_Chr2g0308321	62.475	493	146	11	12	470	52	539	6.93e-173	496
Msa1300180	MtrunA17_Chr5g0420051	49.315	219	88	8	56	264	111	316	3.24e-51	179
Msa1300180	MtrunA17_Chr3g0132091	54.348	138	42	4	75	198	18	148	2.57e-38	142
Msa1300180	MtrunA17_Chr7g0224821	35.016	317	180	8	75	382	26	325	7.31e-37	139
Msa1300180	MtrunA17_Chr8g0386271	47.337	169	70	5	75	232	45	205	3.48e-36	137
Msa1300180	MtrunA17_Chr7g0231911	70.000	80	24	0	117	196	143	222	5.82e-34	130
Msa1300180	MtrunA17_Chr3g0122411	50.769	130	57	2	72	198	115	240	6.74e-34	132
Msa1300180	MtrunA17_Chr7g0258471	69.136	81	25	0	117	197	136	216	2.22e-33	130
Msa1300180	MtrunA17_Chr8g0334671	73.684	76	20	0	123	198	187	262	3.69e-33	128

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1300180	AT1G69170.1	64.286	126	32	3	75	197	83	198	9.59e-45	161
Msa1300180	AT5G50570.1	46.067	178	84	4	123	288	101	278	2.70e-37	140
Msa1300180	AT5G50570.2	46.067	178	84	4	123	288	101	278	2.70e-37	140
Msa1300180	AT5G50670.1	46.067	178	84	4	123	288	101	278	2.70e-37	140
Msa1300180	AT5G50670.2	46.067	178	84	4	123	288	101	278	2.70e-37	140
Msa1300180	AT1G27370.6	68.966	87	27	0	123	209	176	262	2.64e-36	137
Msa1300180	AT1G27370.1	68.966	87	27	0	123	209	176	262	7.10e-36	137
Msa1300180	AT1G27370.5	68.966	87	27	0	123	209	176	262	7.10e-36	137
Msa1300180	AT1G27370.3	68.966	87	27	0	123	209	176	262	7.10e-36	137
Msa1300180	AT1G27370.2	68.966	87	27	0	123	209	176	262	7.10e-36	137

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Find 135 sgRNAs with CRISPR-Local

Find 514 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGATGCTGCTGGTGGCT+TGG	0.511710	8_3:+61626874	None:intergenic
AAAAGTAAACAAGTTTATGT+TGG	0.539447	8_3:+61628961	None:intergenic
AAAATGATGATGAAGGTGTC+AGG	0.533491	8_3:+61629140	None:intergenic
AAACCACTTTGGAAGACCTC+TGG	0.529651	8_3:+61626845	None:intergenic
AAAGCCATTGATGAATAGAT+AGG	0.447792	8_3:+61629234	None:intergenic
AACATGTTAGGCTATATCAA+TGG	0.570957	8_3:-61625330	Msa1300180:three_prime_UTR
AAGCACGTGATGAGAATGGA+TGG	0.562313	8_3:+61629096	None:intergenic
AAGTAGTGAGCAGAAGAGAA+TGG	0.462046	8_3:+61625456	None:intergenic
AAGTCATCTCAGTCCTATAT+TGG	0.441537	8_3:-61626357	Msa1300180:CDS
AATAACCAAAGGGTTATCAA+AGG	0.561243	8_3:+61626478	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAATAGATAGGAAAATGGAA+AGG	+	chr8_3:61625279-61625298	None:intergenic	30.0%
AAAGCCATTGATGAATAGAT+AGG	+	chr8_3:61625291-61625310	None:intergenic	30.0%
TTTCAAGCTTTCAAATTCTG+TGG	-	chr8_3:61625984-61626003	Msa1300180:intron	30.0%
ACTGCATATTGTCAAGTTTA+TGG	-	chr8_3:61626168-61626187	Msa1300180:CDS	30.0%
TCAAGTTTATGGATGTAACA+AGG	-	chr8_3:61626179-61626198	Msa1300180:CDS	30.0%
ATTCCATTCACAATGACTAT+AGG	+	chr8_3:61626267-61626286	None:intergenic	30.0%
ATTTCTAACTAGCTGGAATT+TGG	-	chr8_3:61626400-61626419	Msa1300180:CDS	30.0%
AGGCTAAAAATCAATTGTAG+AGG	+	chr8_3:61626434-61626453	None:intergenic	30.0%
TGACTAAAGAGGTTAATGTT+TGG	+	chr8_3:61626494-61626513	None:intergenic	30.0%
TCATGTTCTAGTATTCCAAT+AGG	+	chr8_3:61626627-61626646	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	61625214	61629330	61625214	ID=Msa1300180;Name=Msa1300180
chr8_3	mRNA	61625214	61629330	61625214	ID=Msa1300180-mRNA-1;Parent=Msa1300180;Name=Msa1300180-mRNA-1;_AED=0.09;_eAED=0.09;_QI=597\|1\|1\|1\|0.66\|0.5\|4\|371\|470
chr8_3	exon	61625214	61625569	61625214	ID=Msa1300180-mRNA-1:exon:18222;Parent=Msa1300180-mRNA-1
chr8_3	exon	61626127	61626914	61626127	ID=Msa1300180-mRNA-1:exon:18221;Parent=Msa1300180-mRNA-1
chr8_3	exon	61627396	61627529	61627396	ID=Msa1300180-mRNA-1:exon:18220;Parent=Msa1300180-mRNA-1
chr8_3	exon	61628228	61629330	61628228	ID=Msa1300180-mRNA-1:exon:18219;Parent=Msa1300180-mRNA-1
chr8_3	five_prime_UTR	61628734	61629330	61628734	ID=Msa1300180-mRNA-1:five_prime_utr;Parent=Msa1300180-mRNA-1
chr8_3	CDS	61628228	61628733	61628228	ID=Msa1300180-mRNA-1:cds;Parent=Msa1300180-mRNA-1
chr8_3	CDS	61627396	61627529	61627396	ID=Msa1300180-mRNA-1:cds;Parent=Msa1300180-mRNA-1
chr8_3	CDS	61626142	61626914	61626142	ID=Msa1300180-mRNA-1:cds;Parent=Msa1300180-mRNA-1
chr8_3	three_prime_UTR	61626127	61626141	61626127	ID=Msa1300180-mRNA-1:three_prime_utr;Parent=Msa1300180-mRNA-1
chr8_3	three_prime_UTR	61625214	61625569	61625214	ID=Msa1300180-mRNA-1:three_prime_utr;Parent=Msa1300180-mRNA-1

Gene Sequence

>Msa1300180

ATGGAATCTTGGAGTTATATTTCTGAAGAAAAAGGTTCTGAAGAATTAGGCTTTTGTGAAATGTTTGGGAAACAATATTCAGATGATTTCAATTTCATTGGAAGTGTTCCAAGCAAGAAACTTGATGGTAAATTTGATGACCCAAGTGAGTTTTCTGTAAAAGGGGACACAAATTTCAAGCTTTCAAATTCTGTGGTGGAATCCAATGGTTGGGATTCTCTCATTGATTTGAAGCTAGGAAGATTTGATGATCATGGCAATGCTTCAATTGATGCGGCATTTTCCAAAGGAGCAGCACCTATTTTGTCTTCTTGTGAGTCTTCAACACCTCCCAAGAGAGTGAGAGTGCACTCAATGACTGCATATTGTCAAGTTTATGGATGTAACAAGGATCTTAGTTCTTGTAAGGACTACCATAAGAGGCATAAAGTTTGTGAGGTTCACTCCAAGACTCCTATAGTCATTGTGAATGGAATTGAGCAGAGGTTTTGTCAACAATGTAGCAGGTTTCATTTGCTGTCTGAGTTTGATGATGGTAAGCGCAGTTGCCGTAAACGACTTGCAGGCCATAATGAGCGTCGAAGAAAACCACAAGGCGTTCACTCTACAAATTCTGGGAGACTGTTTCAGCCATTTGGTGATATCAGATTCCAGGGAACCAAGCCACCAGCAGCATCTTTTCTCTATCCAGAGGTCTTCCAAAGTGGTTTCTGGAGACCTATGAAAGCAGAACATGAAGCTGGCTTTAGGCATTTTTCTTCTGTGCCTGTCACCAATGGACATCTTCAATCAAGATCTCCGTTTCCTTCGTACAACGAAAAACAATTTCCCTTTCTTCATGAAAATGTTGCCACATCCACTACAGGCAGTAAGTTCAGTGAAAACAATAGCCATTATGCTCATGCCATTGGCTACACCTCCTTAGGAAGCGAAGACTTCAATGTTTTTCATACCGCACCAAGCATTCAAGGATTATCAGGAATTTCAGACTCTTGTGCTCTCTCTCGTCTGTCATCTCAGTCCAATAACACTTCAAGCCAGTCCTTGGAAATTCCCTTTGATAACCCTTTGGTTATTCCAAGCAGCCATAGCCATGTCTACAGCATAAGATCACAAGCGACTTCCTCAAGCCGAGTGTCTGATAGACTTTTATCCGAATTAAATCCTACAGATGGAAGTCATCTCAGTCCTATATTGGTACCAGACAATAATGATATCGTGAACTTTGAAATGGAAGACGGGATCTTCCATGATTCAGATTTTGTGAATGTGAAAGACTGCCTTTCTTGTGAAGATGATGCAACCATCGATTTACTACAACTGTCGTCGCAGCTTCAACGAGTTGAGCATCATAGACAATCATTGCAGGTGAAGAAAGAGTTTGATTCTTCTTGCACTCTCCGGATTACTTAA

Protein sequence

>Msa1300180

MESWSYISEEKGSEELGFCEMFGKQYSDDFNFIGSVPSKKLDGKFDDPSEFSVKGDTNFKLSNSVVESNGWDSLIDLKLGRFDDHGNASIDAAFSKGAAPILSSCESSTPPKRVRVHSMTAYCQVYGCNKDLSSCKDYHKRHKVCEVHSKTPIVIVNGIEQRFCQQCSRFHLLSEFDDGKRSCRKRLAGHNERRRKPQGVHSTNSGRLFQPFGDIRFQGTKPPAASFLYPEVFQSGFWRPMKAEHEAGFRHFSSVPVTNGHLQSRSPFPSYNEKQFPFLHENVATSTTGSKFSENNSHYAHAIGYTSLGSEDFNVFHTAPSIQGLSGISDSCALSRLSSQSNNTSSQSLEIPFDNPLVIPSSHSHVYSIRSQATSSSRVSDRLLSELNPTDGSHLSPILVPDNNDIVNFEMEDGIFHDSDFVNVKDCLSCEDDATIDLLQLSSQLQRVEHHRQSLQVKKEFDSSCTLRIT*