AlfalfaGEDB | Msa1300270

M. sativa cultivar zhongmu-4 / Msa1300270

KEGG: K17586
GO: GO:0003674 GO:0005488 GO:0005515 GO:0006139 GO:0006351 GO:0006366 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0018130 GO:0019438 GO:0019899 GO:0019904 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0046483 GO:0070063 GO:0071704 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576
PFAM: PF13639.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1300270	A0A072UQF4	94.337	618	29	4	1	616	1	614	0.0	1179

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TFs/TRs:

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Gene ID	Type	Classification
Msa1300270	TR	PHD

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1213320	Msa1300270	0.860582	1.868285e-63	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1300270	MtrunA17_Chr4g0061801	94.337	618	29	4	1	616	1	614	0.0	1179
Msa1300270	MtrunA17_Chr7g0271951	42.405	158	85	4	32	187	426	579	4.16e-34	139
Msa1300270	MtrunA17_Chr7g0271951	43.000	100	54	2	514	611	794	892	1.32e-13	74.3
Msa1300270	MtrunA17_Chr8g0388341	34.426	183	86	10	32	189	34	207	7.21e-18	88.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1300270	AT3G05670.3	33.091	275	169	8	32	297	413	681	4.39e-36	145
Msa1300270	AT3G05670.1	33.091	275	169	8	32	297	413	681	4.39e-36	145
Msa1300270	AT3G05670.2	33.091	275	169	8	32	297	413	681	4.39e-36	145
Msa1300270	AT4G10930.2	29.193	161	82	7	32	165	31	186	5.73e-12	69.7
Msa1300270	AT4G10930.1	29.193	161	82	7	32	165	31	186	6.04e-12	69.7

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Find 153 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCAATCACCTTCAGGAA+CGG	0.610160	8_3:+61801606	None:intergenic
AAACCTTCAAGGTAGTTGCA+AGG	0.642576	8_3:-61800185	Msa1300270:CDS
AAAGATCACATATTAGTATA+AGG	0.325967	8_3:+61801671	None:intergenic
AAAGCATTGCCGGCAACAAT+TGG	0.364853	8_3:+61800038	None:intergenic
AAATCATCGGTCTCCGATGT+TGG	0.470526	8_3:-61800864	Msa1300270:CDS
AAATTCCACAGCAAACATCT+TGG	0.499946	8_3:+61802828	None:intergenic
AACCATAATACTCATTTAGA+AGG	0.398353	8_3:-61800788	Msa1300270:CDS
AAGAAAAGACATCATGCATA+GGG	0.555505	8_3:+61802663	None:intergenic
AAGAACAAGTAGAATTCAAC+AGG	0.416343	8_3:-61801044	Msa1300270:CDS
AAGACAAATTCGGGACCAGA+CGG	0.632549	8_3:+61802959	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGCAAACGAAGCAAAAATAA+TGG	+	chr8_3:61799965-61799984	None:intergenic	30.0%
AAGAAAAGACATCATGCATA+GGG	+	chr8_3:61800058-61800077	None:intergenic	30.0%
TGTCAACAATTCATGTGTTA+GGG	-	chr8_3:61800227-61800246	Msa1300270:intron	30.0%
TTAGTTTAGGAATTCACTAG+CGG	+	chr8_3:61800333-61800352	None:intergenic	30.0%
ATCTTGACTGAAATTGTAGA+GGG	-	chr8_3:61800373-61800392	Msa1300270:intron	30.0%
TGAAGAACAAAGTCAAATCT+TGG	-	chr8_3:61800435-61800454	Msa1300270:intron	30.0%
GACTAATGCTAATTATATGC+TGG	-	chr8_3:61801161-61801180	Msa1300270:CDS	30.0%
ACAAAATGTTGAGCTAAAAG+CGG	-	chr8_3:61801188-61801207	Msa1300270:CDS	30.0%
ACCATCTTAAATATTGTGAG+AGG	-	chr8_3:61801222-61801241	Msa1300270:CDS	30.0%
TCCTCTCACAATATTTAAGA+TGG	+	chr8_3:61801226-61801245	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	61799735	61803005	61799735	ID=Msa1300270;Name=Msa1300270
chr8_3	mRNA	61799735	61803005	61799735	ID=Msa1300270-mRNA-1;Parent=Msa1300270;Name=Msa1300270-mRNA-1;_AED=0.00;_eAED=0.00;_QI=75\|1\|1\|1\|1\|1\|3\|246\|616
chr8_3	exon	61799735	61800213	61799735	ID=Msa1300270-mRNA-1:exon:18295;Parent=Msa1300270-mRNA-1
chr8_3	exon	61800471	61801785	61800471	ID=Msa1300270-mRNA-1:exon:18294;Parent=Msa1300270-mRNA-1
chr8_3	exon	61802628	61803005	61802628	ID=Msa1300270-mRNA-1:exon:18293;Parent=Msa1300270-mRNA-1
chr8_3	five_prime_UTR	61802931	61803005	61802931	ID=Msa1300270-mRNA-1:five_prime_utr;Parent=Msa1300270-mRNA-1
chr8_3	CDS	61802628	61802930	61802628	ID=Msa1300270-mRNA-1:cds;Parent=Msa1300270-mRNA-1
chr8_3	CDS	61800471	61801785	61800471	ID=Msa1300270-mRNA-1:cds;Parent=Msa1300270-mRNA-1
chr8_3	CDS	61799981	61800213	61799981	ID=Msa1300270-mRNA-1:cds;Parent=Msa1300270-mRNA-1
chr8_3	three_prime_UTR	61799735	61799980	61799735	ID=Msa1300270-mRNA-1:three_prime_utr;Parent=Msa1300270-mRNA-1

Gene Sequence

>Msa1300270

ATGTCGACCTCGTTGCCTACATCAAACAAACGCATCAAAACCGAAGTGAAAACTGAAGAAGAAGACGACAATGGCAATAACCAAGATGTTTGCTGTGGAATTTGCTACATCGAACGCGGAATTTCAGTCCCTGCACAAATTGATTGCTGCAACCATTATTTTTGCTTCGTTTGCATCATGGAATGGGCCAAACATGAATCTCGATGCCCACTTTGTCGCCAGAGATTCTCAAACGTTCGTAGACTCCCTATGCATGATGTCTTTTCTTCCTCACGGGATGTTAAGATTCCTATTCGCGATCAGGTTTACCATCCTCATGGAAATATGACCAGTGGTCCTGTTGATTCTAATGCTGAACGCAAGTGCTGTATTTGCGATGGTGCGAAAGATGAAAACCTTATACTAATATGTGATCTTTGTGATACTGTTTCACATACGTTTTGTGTTGGATTGGGCTACACCGTTCCTGAAGGTGATTGGTTTTGTCATGATTGTGCTGTTTCCCTGGAGACTAATGCTAATTATATGCTGGACCAACAAAATGTTGAGCTAAAAGCGGCGCCTGATGTAACCATCTTAAATATTGTGAGAGGAACAGGCAGCCAAGTGGTTAGAAGACCAATGGCATCTCCTGTACGACAAAACAATTCCTTGTCTTCTGATATTCCTTTACTTGATAGGGTGAGCAGATCCGAAGGTAAGAAACCTGTGTCGGAAGTCCAGCGTGCCCTGCGTAATGTTCAACTGCTTCGTGAAAATTGGAATTCTTTAAGAAGTGGTAAATTAAGGTTCCATAGCAAAACATTTCAATCTGGTGGTACAAGTAGTCAAACAGAAAATTCTAGTTCTTTATCAAGTGGCAAATTAGATGATTCACATTCAATGGCTTCCGCAAGCCGTCAACAATCCACAGTCCAACGTGCTCAATCAAGTAACATGTTGAGTAATGGTGGTTTGAAAGATGACGTTGACAAAGCTTGGAAAATGATGCGTAAGGCAAAGACGATGCAACAGACTCCTAGAAGAACAAGTAGAATTCAACAGGAAGTGGATGATGGTCCCTCATGCAGTGGTGCTAGAGAAAAATCATTTTCACCTCGTAGTTGCCCAGAATCTAAAAAACAACAACCCAGAACATTAGACTTTATGTACGCCAAAATGCAGGAGAAGTGTGACTACTCTTGTCCCCCAAAAAATTTGGAAAATCATCGGTCTCCGATGTTGGGAGAGAAAAGGCAAAGTAGGGTCTTGTGTGAGGAGAAGATACAACATTTGAGAAACCATAATACTCATTTAGAAGGATACAGGGAACGTCCATTGCCCAGTAAGGTCCATACTAGTAGTAGTGCCCACAAAGCTCCCTGTCACAATGAAAGAAATGCCTCAAAGGAACAAAGGCAGTCTGCTTACTTGGTAACTTCAGTAGGTTCAGCACCCTCAAGTGGAAAATCTAGTAGCGTGTTCACCTCTAAGAAGGATGTGGATATTTTCAATAAAGAAAAGAGGTTGGCTAAAAGCTTCGGGAATGGAATCACAAAAAATACTGAAGATGCAAAAACAGAGATCCAATCTCTTGTTAAACTAAATTTGAATTGCTTAGCTATCAATAAACAATTAGGTGTCGAAACCTTCAAGGTAGTTGCAAGGCAAGCCACCCATACCATCTTGGCTGCTTGCCATTCCGAGCAGCAAAAGTCTAATATATATTCCTCGAACTCAGTATGCAGCCATACTGACCATAATCTTCAGTTTCAGAAGTCTAATTTAATGCCCAATTGTTGCCGGCAATGCTTTCATGTCTTCGTGAACGATGTTGTCAAGTCGGTGTTGGAAAAAGTGGGCTGTTCTTGA

Protein sequence

>Msa1300270

MSTSLPTSNKRIKTEVKTEEEDDNGNNQDVCCGICYIERGISVPAQIDCCNHYFCFVCIMEWAKHESRCPLCRQRFSNVRRLPMHDVFSSSRDVKIPIRDQVYHPHGNMTSGPVDSNAERKCCICDGAKDENLILICDLCDTVSHTFCVGLGYTVPEGDWFCHDCAVSLETNANYMLDQQNVELKAAPDVTILNIVRGTGSQVVRRPMASPVRQNNSLSSDIPLLDRVSRSEGKKPVSEVQRALRNVQLLRENWNSLRSGKLRFHSKTFQSGGTSSQTENSSSLSSGKLDDSHSMASASRQQSTVQRAQSSNMLSNGGLKDDVDKAWKMMRKAKTMQQTPRRTSRIQQEVDDGPSCSGAREKSFSPRSCPESKKQQPRTLDFMYAKMQEKCDYSCPPKNLENHRSPMLGEKRQSRVLCEEKIQHLRNHNTHLEGYRERPLPSKVHTSSSAHKAPCHNERNASKEQRQSAYLVTSVGSAPSSGKSSSVFTSKKDVDIFNKEKRLAKSFGNGITKNTEDAKTEIQSLVKLNLNCLAINKQLGVETFKVVARQATHTILAACHSEQQKSNIYSSNSVCSHTDHNLQFQKSNLMPNCCRQCFHVFVNDVVKSVLEKVGCS*