AlfalfaGEDB | Msa1301440

M. sativa cultivar zhongmu-4 / Msa1301440

KEGG: K07964
GO: GO:0003674 GO:0003824 GO:0004553 GO:0004566 GO:0005575 GO:0005618 GO:0005623 GO:0009505 GO:0016787 GO:0016798 GO:0030312 GO:0044464 GO:0071944
PFAM: PF03662.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1301440	XP_003609474.1	94.340	530	27	2	1	528	1	529	0.0	1007

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1301440	sp\|Q9FZP1\|HPSE3_ARATH	59.707	546	192	6	1	528	1	536	0.0	678

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1301440	G7JGL1	94.340	530	27	2	1	528	1	529	0.0	1007

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1301440	Msa1448390	0.800311	1.529688e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1301440	MtrunA17_Chr4g0064851	94.340	530	27	2	1	528	1	529	0.0	1007
Msa1301440	MtrunA17_Chr2g0304251	66.034	527	163	4	15	527	12	536	0.0	713
Msa1301440	MtrunA17_Chr3g0086271	49.023	512	253	5	21	528	8	515	5.91e-173	498
Msa1301440	MtrunA17_Chr3g0086291	50.302	497	236	6	39	528	25	517	2.11e-172	497
Msa1301440	MtrunA17_Chr3g0086241	47.706	545	253	9	14	528	1	543	3.06e-172	497
Msa1301440	MtrunA17_Chr4g0061271	48.069	518	240	5	39	527	25	542	3.26e-166	482
Msa1301440	MtrunA17_Chr3g0086251	48.362	519	259	7	14	528	111	624	4.04e-165	482
Msa1301440	MtrunA17_Chr5g0418671	45.769	520	257	5	11	527	6	503	7.36e-163	472
Msa1301440	MtrunA17_Chr4g0064821	67.544	228	60	3	168	395	3	216	1.47e-103	310
Msa1301440	MtrunA17_Chr4g0064811	74.257	101	25	1	428	528	1	100	1.11e-43	150

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1301440	AT5G34940.2	59.707	546	192	6	1	528	1	536	0.0	678
Msa1301440	AT5G34940.1	60.848	401	141	2	144	528	1	401	0.0	515
Msa1301440	AT5G61250.3	46.887	514	250	3	38	528	26	539	1.14e-173	501
Msa1301440	AT5G61250.1	46.887	514	250	3	38	528	26	539	1.14e-173	501
Msa1301440	AT5G61250.2	46.887	514	250	3	38	528	26	539	1.14e-173	501
Msa1301440	AT5G34940.3	63.585	357	128	1	35	389	25	381	1.47e-173	494
Msa1301440	AT5G07830.1	47.379	515	247	4	38	528	29	543	6.13e-171	494
Msa1301440	AT5G34940.4	59.732	298	104	2	247	528	2	299	1.47e-127	374

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Find 146 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCACTAATCATTGCACC+TGG	0.631010	8_3:-63453581	Msa1301440:CDS
AAACCATCCACTCATAATGT+GGG	0.621064	8_3:-63452456	Msa1301440:CDS
AAAGGGGTTGTTGTAATTCA+TGG	0.447039	8_3:-63454904	Msa1301440:CDS
AAATGATTCAGCTGTGAAAG+GGG	0.633658	8_3:-63454920	Msa1301440:CDS
AAATTTACATAGCCAAACTA+TGG	0.453369	8_3:-63452391	Msa1301440:CDS
AACCGAAAATCGGTTTCCCT+TGG	0.491824	8_3:-63452193	Msa1301440:three_prime_UTR
AACTTATGCACATTGTGCAA+AGG	0.615463	8_3:-63452683	Msa1301440:CDS
AAGAACCTCAGCTCAAGCAT+GGG	0.599111	8_3:-63453278	Msa1301440:CDS
AAGGTCCAACAGCAGAACTC+TGG	0.458755	8_3:+63454065	None:intergenic
AATCAAAGTTAAGATCTTTA+TGG	0.167612	8_3:+63455049	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TACATCCAAAAAAACATACC+CGG	+	chr8_3:63452209-63452228	None:intergenic	30.0%
TTGTTTGTGCAACTATTGAT+TGG	-	chr8_3:63452351-63452370	Msa1301440:CDS	30.0%
TTCACCCTTGAAAATTAGAT+TGG	-	chr8_3:63452753-63452772	Msa1301440:CDS	30.0%
TCACCCTTGAAAATTAGATT+GGG	-	chr8_3:63452754-63452773	Msa1301440:CDS	30.0%
ATATGTGACTTTATCTTGCA+AGG	+	chr8_3:63452785-63452804	None:intergenic	30.0%
TTGCAAGATAAAGTCACATA+TGG	-	chr8_3:63452784-63452803	Msa1301440:intron	30.0%
TGCAAGATAAAGTCACATAT+GGG	-	chr8_3:63452785-63452804	Msa1301440:intron	30.0%
TTCAAAACAAATGGAGTACA+AGG	+	chr8_3:63452829-63452848	None:intergenic	30.0%
GTTTACCATTAATCAATGGT+AGG	-	chr8_3:63453051-63453070	Msa1301440:CDS	30.0%
ATCAATGGTAGGTATATTTC+AGG	-	chr8_3:63453062-63453081	Msa1301440:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	63451963	63455259	63451963	ID=Msa1301440;Name=Msa1301440
chr8_3	mRNA	63451963	63455259	63451963	ID=Msa1301440-mRNA-1;Parent=Msa1301440;Name=Msa1301440-mRNA-1;_AED=0.22;_eAED=0.22;_QI=222\|1\|1\|1\|1\|1\|9\|276\|528
chr8_3	exon	63451963	63452556	63451963	ID=Msa1301440-mRNA-1:exon:19011;Parent=Msa1301440-mRNA-1
chr8_3	exon	63452678	63452777	63452678	ID=Msa1301440-mRNA-1:exon:19010;Parent=Msa1301440-mRNA-1
chr8_3	exon	63452996	63453121	63452996	ID=Msa1301440-mRNA-1:exon:19009;Parent=Msa1301440-mRNA-1
chr8_3	exon	63453208	63453397	63453208	ID=Msa1301440-mRNA-1:exon:19008;Parent=Msa1301440-mRNA-1
chr8_3	exon	63453486	63453716	63453486	ID=Msa1301440-mRNA-1:exon:19007;Parent=Msa1301440-mRNA-1
chr8_3	exon	63453990	63454138	63453990	ID=Msa1301440-mRNA-1:exon:19006;Parent=Msa1301440-mRNA-1
chr8_3	exon	63454290	63454473	63454290	ID=Msa1301440-mRNA-1:exon:19005;Parent=Msa1301440-mRNA-1
chr8_3	exon	63454583	63454619	63454583	ID=Msa1301440-mRNA-1:exon:19004;Parent=Msa1301440-mRNA-1
chr8_3	exon	63454786	63455259	63454786	ID=Msa1301440-mRNA-1:exon:19003;Parent=Msa1301440-mRNA-1
chr8_3	five_prime_UTR	63455038	63455259	63455038	ID=Msa1301440-mRNA-1:five_prime_utr;Parent=Msa1301440-mRNA-1
chr8_3	CDS	63454786	63455037	63454786	ID=Msa1301440-mRNA-1:cds;Parent=Msa1301440-mRNA-1
chr8_3	CDS	63454583	63454619	63454583	ID=Msa1301440-mRNA-1:cds;Parent=Msa1301440-mRNA-1
chr8_3	CDS	63454290	63454473	63454290	ID=Msa1301440-mRNA-1:cds;Parent=Msa1301440-mRNA-1
chr8_3	CDS	63453990	63454138	63453990	ID=Msa1301440-mRNA-1:cds;Parent=Msa1301440-mRNA-1
chr8_3	CDS	63453486	63453716	63453486	ID=Msa1301440-mRNA-1:cds;Parent=Msa1301440-mRNA-1
chr8_3	CDS	63453208	63453397	63453208	ID=Msa1301440-mRNA-1:cds;Parent=Msa1301440-mRNA-1
chr8_3	CDS	63452996	63453121	63452996	ID=Msa1301440-mRNA-1:cds;Parent=Msa1301440-mRNA-1
chr8_3	CDS	63452678	63452777	63452678	ID=Msa1301440-mRNA-1:cds;Parent=Msa1301440-mRNA-1
chr8_3	CDS	63452239	63452556	63452239	ID=Msa1301440-mRNA-1:cds;Parent=Msa1301440-mRNA-1
chr8_3	three_prime_UTR	63451963	63452238	63451963	ID=Msa1301440-mRNA-1:three_prime_utr;Parent=Msa1301440-mRNA-1

Gene Sequence

>Msa1301440

ATGGGTTATCAAAAAAAGCTTCCGGGTATGTTTTTTTGGATGTACTTGGTAAGCTTCAGTTTCAGCTTCATTGGAGTGAATTGCATAGGATTAGGAAATGATTCAGCTGTGAAAGGGGTTGTTGTAATTCATGGCAAAGCTTTTATTGGAAGGATTGATAATGATTTTGTTTGTGCAACTATTGATTGGTGGCCACCTCAGAAATGTGACTGGGGAAGATGCAGTTGGGGTCATGCTTCTCTTCTTAATCTGGACCTCAACAACAAAATTTTGTTAAATGCAATAAAAGCCTTTTCACCCTTGAAAATTAGATTGGGTGGTACCTTGCAAGATAAAGTCACATATGGGACTGAAGATAATAAACAACCTTGTACTCCATTTGTTTTGAAAGAAAATGTTAAGTTTGGTTTCACTCAAGGGTGCTTACCAATGCAAAGATGGGATGAGCTAAACAGCTTTTTCCAAAAAGCAGGGGTTAATGTTATATTTGGATTAAATGCTCTTGCTGGAAGATCTTTTGAATCCCAGAGTTCTGCTGTTGGACCTTGGAACTACACTAATGCCGAGTCTTTTATACGTTATACTGTTGCAAAGAAGTACACTATTCATGGTTGGGAATTCGGTAATGAATTGTGTGGAGCTGGAACTGGAACAAGTGTAGCTGCAGATCAATATGCTTCTGATGTAATTGTTTTAAGAAAGATAGTTCAGGATGTATATCGAAGGGTTAAGCCAAAACCACTAATCATTGCACCTGGAGGCTTCTTTGAGGCAAATTGGTTTAAGGAATTCTTAAACAAATCGGACGAATCCACAAATGTGGTCACCCACCACATTTATAACCTTGGACCAGGAGGTGATCCGGGCTTAGTTGAAAGAATTCTCAATCCATCCTCTCTTAATGGAGTGGCTGACATATTCAGCAGCCTTAAAAATGTACTTCAAAGTTCAAGAACCTCAGCTCAAGCATGGGTTGGTGAAGCAGGAGGGGCTTGGGGCAGTGGTCATCATCTGGTGTCTGATGCATTTGTCAACAGCTTTTGGTATTTGGATCAGCTTGGCATGTCAGCTACTTATGGCACAAGAACATACTGCAGACAGACTTTGATAGGAGGAAACTATGGTTTATTGAATACCACTACTTTCATGCCAAATCCAGATTATTATAGTGCTCTTCTTTGGCACCGACTCATGGGAGGACGTGTCCTGTCAGCTACCTTCTATGGAACAAAGAAGATCAGAACTTATGCACATTGTGCAAAGGAATCTAAGGGAATCACAATACTGTTTCTCAATTTGGACAATAGCACCACTGTTCAAGCCAGAGTGGCCTTAAATTTTGCCAAGAAACCATCCACTCATAATGTGGGAGAATCACAAAGAAGAGAGTACCATTTAACAGCAAAGGATGGAAATTTACATAGCCAAACTATGGTACTAAATGGAAACATTCTAAGTGTAGATTCAGCTGGTGATATTCCTTCACTGAATCCTATATATGTTGATTCTTCAAAGCCAATAACAGTTAGGCCTCTTTCTATTGTTTTTGCTCATATACCAGATGCTGCTGTTAAAGCTTGCAGCTAG

Protein sequence

>Msa1301440

MGYQKKLPGMFFWMYLVSFSFSFIGVNCIGLGNDSAVKGVVVIHGKAFIGRIDNDFVCATIDWWPPQKCDWGRCSWGHASLLNLDLNNKILLNAIKAFSPLKIRLGGTLQDKVTYGTEDNKQPCTPFVLKENVKFGFTQGCLPMQRWDELNSFFQKAGVNVIFGLNALAGRSFESQSSAVGPWNYTNAESFIRYTVAKKYTIHGWEFGNELCGAGTGTSVAADQYASDVIVLRKIVQDVYRRVKPKPLIIAPGGFFEANWFKEFLNKSDESTNVVTHHIYNLGPGGDPGLVERILNPSSLNGVADIFSSLKNVLQSSRTSAQAWVGEAGGAWGSGHHLVSDAFVNSFWYLDQLGMSATYGTRTYCRQTLIGGNYGLLNTTTFMPNPDYYSALLWHRLMGGRVLSATFYGTKKIRTYAHCAKESKGITILFLNLDNSTTVQARVALNFAKKPSTHNVGESQRREYHLTAKDGNLHSQTMVLNGNILSVDSAGDIPSLNPIYVDSSKPITVRPLSIVFAHIPDAAVKACS*