Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1303950 | I3SK06 | 98.000 | 50 | 1 | 0 | 1 | 50 | 1 | 50 | 1.41e-25 | 101 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1083880 | Msa1303950 | 0.818332 | 2.071269e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1303950 | MtrunA17_Chr4g0070471 | 98.000 | 50 | 1 | 0 | 1 | 50 | 1 | 50 | 2.72e-29 | 101 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1303950 | AT2G06010.1 | 75.000 | 32 | 8 | 0 | 19 | 50 | 14 | 45 | 1.99e-12 | 58.5 |
Find 13 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAATTCCGTAAAGAAATTC+AGG | 0.216585 | 8_3:+67249799 | Msa1303950:CDS |
| AGTTCTCTTCTCTTCTCTAA+TGG | 0.231191 | 8_3:+67249679 | Msa1303950:exon |
| GAACACCCTGAATTTCTTTA+CGG | 0.280424 | 8_3:-67249805 | None:intergenic |
| AACTCATCCCACGAAGTTGC+AGG | 0.415261 | 8_3:-67249740 | None:intergenic |
| TGTTCGTGTTGCTCTCAATA+TGG | 0.435898 | 8_3:+67249823 | Msa1303950:CDS |
| GAGAAGAGAACTAAGCAATC+TGG | 0.465231 | 8_3:-67249668 | None:intergenic |
| CTCAAACTCAAACGTGGAAG+AGG | 0.496704 | 8_3:+67249715 | Msa1303950:CDS |
| AAGAGGAACCTGCAACTTCG+TGG | 0.540479 | 8_3:+67249732 | Msa1303950:CDS |
| AAATTCCGTAAAGAAATTCA+GGG | 0.598934 | 8_3:+67249800 | Msa1303950:CDS |
| AGAGGAACCTGCAACTTCGT+GGG | 0.608732 | 8_3:+67249733 | Msa1303950:CDS |
| AATTTGAGAAATAACTCAGA+AGG | 0.628478 | 8_3:-67249782 | None:intergenic |
| TCGTGTTGCTCTCAATATGG+AGG | 0.662474 | 8_3:+67249826 | Msa1303950:CDS |
| TTCGAACTCAAACTCAAACG+TGG | 0.704750 | 8_3:+67249709 | Msa1303950:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATTTGAGAAATAACTCAGA+AGG | - | chr8_3:67249785-67249804 | None:intergenic | 25.0% |
| ! | AAATTCCGTAAAGAAATTCA+GGG | + | chr8_3:67249800-67249819 | Msa1303950:CDS | 25.0% |
| CAAATTCCGTAAAGAAATTC+AGG | + | chr8_3:67249799-67249818 | Msa1303950:CDS | 30.0% | |
| AGTAATAGAGAAAAATTGCG+AGG | - | chr8_3:67249860-67249879 | None:intergenic | 30.0% | |
| AACAACGTCTGTGTTAATTA+GGG | - | chr8_3:67249946-67249965 | None:intergenic | 30.0% | |
| TAACAACGTCTGTGTTAATT+AGG | - | chr8_3:67249947-67249966 | None:intergenic | 30.0% | |
| AGTTCTCTTCTCTTCTCTAA+TGG | + | chr8_3:67249679-67249698 | Msa1303950:exon | 35.0% | |
| GAACACCCTGAATTTCTTTA+CGG | - | chr8_3:67249808-67249827 | None:intergenic | 35.0% | |
| GAGAAGAGAACTAAGCAATC+TGG | - | chr8_3:67249671-67249690 | None:intergenic | 40.0% | |
| TTCGAACTCAAACTCAAACG+TGG | + | chr8_3:67249709-67249728 | Msa1303950:CDS | 40.0% | |
| ! | TGTTCGTGTTGCTCTCAATA+TGG | + | chr8_3:67249823-67249842 | Msa1303950:CDS | 40.0% |
| CTCAAACTCAAACGTGGAAG+AGG | + | chr8_3:67249715-67249734 | Msa1303950:CDS | 45.0% | |
| ! | TCGTGTTGCTCTCAATATGG+AGG | + | chr8_3:67249826-67249845 | Msa1303950:CDS | 45.0% |
| AAGAGGAACCTGCAACTTCG+TGG | + | chr8_3:67249732-67249751 | Msa1303950:CDS | 50.0% | |
| AGAGGAACCTGCAACTTCGT+GGG | + | chr8_3:67249733-67249752 | Msa1303950:CDS | 50.0% | |
| AACTCATCCCACGAAGTTGC+AGG | - | chr8_3:67249743-67249762 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_3 | gene | 67249649 | 67249978 | 67249649 | ID=Msa1303950;Name=Msa1303950 |
| chr8_3 | mRNA | 67249649 | 67249978 | 67249649 | ID=Msa1303950-mRNA-1;Parent=Msa1303950;Name=Msa1303950-mRNA-1;_AED=0.50;_eAED=0.50;_QI=49|0|0.5|0.5|1|1|2|0|56 |
| chr8_3 | exon | 67249649 | 67249847 | 67249649 | ID=Msa1303950-mRNA-1:exon:20587;Parent=Msa1303950-mRNA-1 |
| chr8_3 | exon | 67249958 | 67249978 | 67249958 | ID=Msa1303950-mRNA-1:exon:20588;Parent=Msa1303950-mRNA-1 |
| chr8_3 | five_prime_UTR | 67249649 | 67249697 | 67249649 | ID=Msa1303950-mRNA-1:five_prime_utr;Parent=Msa1303950-mRNA-1 |
| chr8_3 | CDS | 67249698 | 67249847 | 67249698 | ID=Msa1303950-mRNA-1:cds;Parent=Msa1303950-mRNA-1 |
| chr8_3 | CDS | 67249958 | 67249978 | 67249958 | ID=Msa1303950-mRNA-1:cds;Parent=Msa1303950-mRNA-1 |
| Gene Sequence |
| Protein sequence |
