AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1307430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1307430 MtrunA17_Chr8g0345691 74.516 310 50 4 1 281 1 310 1.13e-144 421
Msa1307430 MtrunA17_Chr8g0345691 83.784 222 28 2 277 490 198 419 1.80e-123 367
Msa1307430 MtrunA17_Chr8g0345671 54.517 321 105 7 1 281 1 320 1.32e-92 287
Msa1307430 MtrunA17_Chr8g0345671 52.155 232 62 4 277 490 198 398 2.44e-61 206
Msa1307430 MtrunA17_Chr8g0345721 39.548 177 55 3 1 127 26 200 1.11e-32 124
Msa1307430 MtrunA17_Chr8g0345741 65.854 82 28 0 58 139 58 139 1.41e-32 121
Msa1307430 MtrunA17_Chr8g0345711 38.961 154 72 3 1 132 1 154 5.02e-27 107
Msa1307430 MtrunA17_Chr5g0428991 33.191 235 104 11 8 208 21 236 2.86e-24 106
Msa1307430 MtrunA17_Chr3g0128151 38.650 163 72 6 8 144 18 178 4.52e-23 102
Msa1307430 MtrunA17_Chr3g0139331 40.397 151 66 6 12 139 11 160 6.44e-23 99.8
Msa1307430 MtrunA17_Chr8g0345731 36.478 159 65 4 10 139 22 173 6.45e-23 95.5
Msa1307430 MtrunA17_Chr3g0128111 39.286 168 70 6 15 156 45 206 5.85e-22 95.1
Msa1307430 MtrunA17_Chr6g0481091 39.869 153 64 7 12 138 6 156 3.34e-20 89.7
Msa1307430 MtrunA17_Chr3g0109821 35.359 181 88 6 12 165 6 184 7.38e-20 92.8
Msa1307430 MtrunA17_Chr4g0018391 36.538 156 72 6 8 138 3 156 2.57e-19 89.4
Msa1307430 MtrunA17_Chr7g0214071 33.333 153 83 5 11 144 14 166 3.16e-19 87.4
Msa1307430 MtrunA17_Chr1g0200561 36.842 152 69 6 12 138 19 168 3.43e-19 89.0
Msa1307430 MtrunA17_Chr2g0312321 34.756 164 82 5 12 152 11 172 6.36e-19 87.4
Msa1307430 MtrunA17_Chr6g0484441 34.337 166 71 7 10 145 3 160 7.31e-19 87.4
Msa1307430 MtrunA17_Chr8g0385991 34.194 155 73 5 9 135 11 164 7.35e-19 85.5
Msa1307430 MtrunA17_Chr4g0054791 35.849 212 94 11 12 189 6 209 9.64e-19 87.0
Msa1307430 MtrunA17_Chr5g0400461 34.146 164 80 5 12 147 6 169 1.03e-18 87.4
Msa1307430 MtrunA17_Chr2g0316971 37.594 133 54 3 11 119 22 149 1.41e-18 87.4
Msa1307430 MtrunA17_Chr8g0345701 37.086 151 56 6 12 127 7 153 1.48e-18 83.2
Msa1307430 MtrunA17_Chr5g0398971 36.842 171 75 8 11 152 6 172 2.18e-18 86.7
Msa1307430 MtrunA17_Chr2g0309891 35.484 155 71 6 12 139 6 158 2.38e-18 86.3
Msa1307430 MtrunA17_Chr4g0056541 35.260 173 83 7 8 153 3 173 2.61e-18 86.7
Msa1307430 MtrunA17_Chr1g0197361 30.627 271 125 13 15 248 11 255 4.42e-18 87.4
Msa1307430 MtrunA17_Chr1g0147421 34.211 152 75 5 12 139 27 177 9.59e-18 84.7
Msa1307430 MtrunA17_Chr4g0017921 38.312 154 63 8 12 138 8 156 1.01e-17 84.7
Msa1307430 MtrunA17_Chr1g0183741 33.557 149 79 4 12 140 10 158 1.16e-17 83.2
Msa1307430 MtrunA17_Chr5g0433781 35.714 154 71 6 12 139 6 157 1.42e-17 85.9
Msa1307430 MtrunA17_Chr2g0318101 29.878 164 80 5 11 139 14 177 2.36e-17 83.6
Msa1307430 MtrunA17_Chr4g0047841 31.655 139 76 4 11 130 14 152 6.31e-17 82.0
Msa1307430 MtrunA17_Chr7g0261411 34.483 145 75 4 12 136 10 154 8.75e-17 80.9
Msa1307430 MtrunA17_Chr3g0144271 37.008 127 60 4 12 118 23 149 8.96e-17 82.4
Msa1307430 MtrunA17_Chr5g0405211 34.868 152 72 5 12 138 8 157 1.00e-16 81.6
Msa1307430 MtrunA17_Chr2g0309331 29.098 244 125 11 12 218 10 242 1.87e-16 80.5
Msa1307430 MtrunA17_Chr3g0091921 36.129 155 69 8 12 139 16 167 1.95e-16 80.1
Msa1307430 MtrunA17_Chr3g0092671 36.424 151 68 5 15 139 8 156 3.04e-16 81.3
Msa1307430 MtrunA17_Chr4g0060931 33.548 155 73 7 12 139 17 168 1.06e-15 78.6
Msa1307430 MtrunA17_Chr8g0372121 34.211 152 73 6 12 138 6 155 1.33e-15 77.8
Msa1307430 MtrunA17_Chr6g0461661 34.848 132 57 4 12 119 18 144 1.51e-15 78.2
Msa1307430 MtrunA17_Chr8g0359921 30.233 215 117 10 12 197 14 224 1.65e-15 77.8
Msa1307430 MtrunA17_Chr5g0417001 32.749 171 80 6 9 145 10 179 2.13e-15 77.8
Msa1307430 MtrunA17_Chr7g0253011 36.090 133 61 6 8 118 14 144 2.15e-15 77.8
Msa1307430 MtrunA17_Chr7g0263931 32.051 156 78 5 11 138 10 165 2.82e-15 77.0
Msa1307430 MtrunA17_Chr8g0361731 33.939 165 74 6 15 145 17 180 3.05e-15 77.4
Msa1307430 MtrunA17_Chr7g0217721 36.923 130 51 6 14 119 21 143 5.95e-15 76.3
Msa1307430 MtrunA17_Chr1g0201021 34.078 179 74 8 15 153 22 196 1.04e-14 76.6
Msa1307430 MtrunA17_Chr4g0076731 35.294 153 74 6 10 139 52 202 1.13e-14 75.9
Msa1307430 MtrunA17_Chr4g0042561 35.032 157 68 7 12 138 9 161 1.26e-14 73.9
Msa1307430 MtrunA17_Chr4g0038751 33.793 145 76 5 15 139 18 162 1.42e-14 74.3
Msa1307430 MtrunA17_Chr3g0113001 31.694 183 97 9 36 205 3 170 2.49e-14 73.6
Msa1307430 MtrunA17_Chr2g0326361 32.692 156 75 5 13 139 15 169 5.43e-14 73.2
Msa1307430 MtrunA17_Chr1g0197351 32.278 158 69 6 15 138 45 198 6.51e-14 73.9
Msa1307430 MtrunA17_Chr7g0217711 36.434 129 51 6 14 118 21 142 1.18e-13 69.3
Msa1307430 MtrunA17_Chr4g0050701 28.221 163 95 5 12 152 11 173 1.96e-13 68.9
Msa1307430 MtrunA17_Chr2g0321741 40.964 83 44 3 59 140 58 136 2.08e-13 71.6
Msa1307430 MtrunA17_Chr3g0113031 39.806 103 50 5 55 147 1 101 2.49e-13 70.1
Msa1307430 MtrunA17_Chr3g0131911 32.515 163 80 6 12 144 14 176 5.42e-13 68.9
Msa1307430 MtrunA17_Chr7g0243371 42.623 61 34 1 59 118 29 89 2.72e-12 63.5
Msa1307430 MtrunA17_Chr8g0384351 32.075 159 85 6 12 155 10 160 3.67e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1307430 AT4G01550.1 41.011 178 71 7 14 164 6 176 1.95e-26 112
Msa1307430 AT4G01520.1 31.441 229 115 8 13 207 5 225 8.04e-23 99.0
Msa1307430 AT3G49530.2 38.415 164 64 8 8 140 9 166 2.96e-22 99.4
Msa1307430 AT3G49530.1 38.415 164 64 8 8 140 9 166 5.76e-22 99.0
Msa1307430 AT4G01540.3 31.579 228 114 8 14 207 6 225 3.19e-21 96.3
Msa1307430 AT4G01540.4 31.579 228 114 8 14 207 6 225 3.41e-21 95.9
Msa1307430 AT4G01540.2 31.579 228 114 8 14 207 6 225 4.06e-21 95.9
Msa1307430 AT4G01540.1 31.579 228 114 8 14 207 6 225 5.24e-21 96.3
Msa1307430 AT4G17980.1 36.306 157 72 5 12 140 6 162 2.40e-20 91.3
Msa1307430 AT3G10490.1 37.086 151 66 6 15 138 30 178 3.10e-20 90.1
Msa1307430 AT5G24590.2 36.420 162 70 6 8 140 9 166 3.22e-20 93.6
Msa1307430 AT1G12260.1 35.714 168 81 6 8 150 3 168 3.25e-20 92.8
Msa1307430 AT4G17980.2 36.306 157 72 5 12 140 6 162 3.44e-20 91.3
Msa1307430 AT3G10490.2 37.086 151 66 6 15 138 30 178 3.90e-20 93.2
Msa1307430 AT1G12260.2 35.714 168 81 6 8 150 3 168 4.26e-20 92.0
Msa1307430 AT4G35580.3 35.404 161 76 5 8 142 5 163 5.78e-20 93.2
Msa1307430 AT4G35580.2 35.404 161 76 5 8 142 5 163 6.02e-20 93.2
Msa1307430 AT4G35580.1 35.404 161 76 5 8 142 5 163 8.19e-20 92.8
Msa1307430 AT3G10480.1 36.424 151 67 6 15 138 30 178 8.19e-20 92.4
Msa1307430 AT3G10480.2 36.667 150 67 6 15 138 30 177 8.45e-20 92.4
Msa1307430 AT5G46590.1 38.994 159 69 7 12 143 6 163 1.87e-19 89.0
Msa1307430 AT1G33060.2 36.306 157 72 5 8 138 20 174 5.15e-19 90.9
Msa1307430 AT1G33060.1 36.306 157 72 5 8 138 20 174 5.30e-19 90.5
Msa1307430 AT1G62700.1 36.538 156 72 6 8 138 3 156 7.12e-19 89.0
Msa1307430 AT1G62700.2 36.538 156 72 6 8 138 3 156 7.12e-19 89.0
Msa1307430 AT3G17730.1 36.842 171 76 8 12 152 6 174 9.12e-19 86.3
Msa1307430 AT1G76420.1 35.294 153 70 5 12 138 22 171 1.78e-18 87.0
Msa1307430 AT2G24430.2 34.177 158 69 6 12 140 16 167 2.21e-18 86.3
Msa1307430 AT2G24430.1 34.177 158 69 6 12 140 16 167 2.21e-18 86.3
Msa1307430 AT1G33280.1 37.086 151 69 6 12 138 8 156 3.00e-18 85.9
Msa1307430 AT3G15170.1 38.065 155 68 6 12 139 20 173 3.06e-18 85.9
Msa1307430 AT5G17260.1 37.662 154 67 6 12 138 6 157 3.91e-18 87.4
Msa1307430 AT3G18400.1 36.478 159 74 8 10 143 3 159 4.92e-18 85.5
Msa1307430 AT1G65910.1 38.312 154 67 6 12 139 6 157 5.36e-18 87.4
Msa1307430 AT3G03200.1 36.364 154 69 6 12 138 6 157 5.43e-18 87.0
Msa1307430 AT5G39610.1 35.065 154 70 6 12 138 20 170 8.20e-18 84.3
Msa1307430 AT5G62380.2 29.310 232 119 8 8 207 3 221 2.07e-17 84.0
Msa1307430 AT5G62380.1 29.310 232 119 8 8 207 3 221 2.07e-17 84.0
Msa1307430 AT5G04410.1 35.526 152 68 6 15 139 12 160 2.36e-17 85.5
Msa1307430 AT1G32510.1 35.119 168 73 7 12 145 6 171 3.81e-17 82.0
Msa1307430 AT3G15510.1 30.857 175 84 7 16 153 21 195 4.94e-17 83.2
Msa1307430 AT2G18060.3 33.742 163 81 5 12 149 9 169 5.57e-17 82.8
Msa1307430 AT2G18060.1 33.742 163 81 5 12 149 9 169 5.57e-17 82.8
Msa1307430 AT3G12977.1 32.597 181 87 7 12 163 18 192 6.14e-17 81.6
Msa1307430 AT4G27410.2 29.952 207 105 9 12 189 14 209 6.80e-17 81.6
Msa1307430 AT5G46590.2 39.535 129 56 5 12 119 6 133 9.48e-17 80.9
Msa1307430 AT5G13180.1 32.432 148 80 4 11 138 13 160 9.98e-17 80.5
Msa1307430 AT3G10500.1 36.424 151 68 6 15 139 12 160 1.05e-16 83.2
Msa1307430 AT5G18270.2 33.117 154 74 5 12 138 21 172 1.33e-16 81.3
Msa1307430 AT5G18270.1 33.117 154 74 5 12 138 21 172 1.37e-16 81.3
Msa1307430 AT4G10350.1 34.524 168 80 7 12 151 9 174 1.69e-16 81.3
Msa1307430 AT3G04060.1 35.065 154 71 7 12 138 20 171 1.80e-16 80.9
Msa1307430 AT3G10480.3 43.678 87 40 3 59 138 127 211 2.24e-16 82.0
Msa1307430 AT1G56010.2 32.500 160 68 5 12 138 19 171 2.53e-16 80.5
Msa1307430 AT1G79580.3 36.842 152 69 7 12 138 17 166 2.66e-16 80.9
Msa1307430 AT1G79580.5 36.842 152 69 7 12 138 17 166 2.66e-16 80.9
Msa1307430 AT1G79580.4 36.842 152 69 7 12 138 17 166 2.66e-16 80.9
Msa1307430 AT1G79580.2 36.842 152 69 7 12 138 17 166 2.66e-16 80.9
Msa1307430 AT1G79580.1 36.842 152 69 7 12 138 17 166 2.66e-16 80.9
Msa1307430 AT5G66300.1 33.987 153 73 6 12 138 12 162 2.79e-16 79.7
Msa1307430 AT1G61110.1 32.143 168 77 7 7 138 11 177 2.80e-16 80.1
Msa1307430 AT5G53950.1 38.281 128 58 3 11 118 16 142 4.75e-16 80.1
Msa1307430 AT3G15500.1 29.302 215 118 8 12 199 14 221 4.78e-16 79.3
Msa1307430 AT4G36160.1 34.211 152 73 5 12 138 10 159 5.79e-16 80.1
Msa1307430 AT4G36160.3 34.211 152 73 5 12 138 10 159 5.79e-16 80.1
Msa1307430 AT1G26870.1 35.849 159 67 6 15 139 19 176 6.83e-16 80.1
Msa1307430 AT4G36160.2 34.211 152 73 5 12 138 14 163 7.09e-16 79.7
Msa1307430 AT3G10490.4 46.250 80 34 3 66 138 1 78 7.32e-16 79.3
Msa1307430 AT3G10490.3 46.250 80 34 3 66 138 1 78 7.32e-16 79.3
Msa1307430 AT1G52890.1 31.073 177 92 7 6 156 8 180 8.91e-16 78.6
Msa1307430 AT3G29035.1 39.370 127 54 6 12 118 24 147 1.05e-15 78.6
Msa1307430 AT1G01720.1 32.911 158 75 6 9 138 4 158 1.08e-15 78.2
Msa1307430 AT4G28530.1 30.303 165 79 5 12 140 10 174 1.37e-15 78.6
Msa1307430 AT3G12977.2 38.261 115 56 5 59 163 30 139 3.68e-15 75.1
Msa1307430 AT2G33480.2 33.108 148 76 6 11 138 14 158 4.68e-15 75.9
Msa1307430 AT1G34190.1 35.294 153 67 7 15 139 19 167 4.82e-15 78.2
Msa1307430 AT5G39820.1 37.037 135 66 4 10 125 18 152 5.79e-15 76.6
Msa1307430 AT4G27410.3 28.571 224 103 10 12 189 14 226 7.73e-15 75.9
Msa1307430 AT2G33480.1 32.667 150 76 6 11 138 14 160 8.61e-15 75.1
Msa1307430 AT1G56010.1 41.573 89 41 3 59 138 18 104 9.38e-15 74.7
Msa1307430 AT2G17040.1 32.099 162 84 6 15 150 9 170 1.92e-14 73.9
Msa1307430 AT1G54330.1 36.667 150 70 6 12 138 6 153 2.17e-14 74.3
Msa1307430 AT2G02450.2 37.415 147 67 7 15 138 54 198 2.41e-14 75.5
Msa1307430 AT2G02450.1 37.415 147 67 7 15 138 54 198 3.28e-14 74.7
Msa1307430 AT5G61430.2 42.697 89 41 5 59 139 18 104 2.24e-13 70.9
Msa1307430 AT4G10350.2 41.000 100 50 4 59 151 30 127 2.38e-13 71.2
Msa1307430 AT1G34180.3 33.775 151 72 5 15 139 19 167 2.92e-13 72.0
Msa1307430 AT1G34180.1 33.113 151 73 5 15 139 19 167 6.14e-13 71.6
Msa1307430 AT5G22380.1 32.278 158 74 6 10 135 3 159 7.80e-13 68.6
Msa1307430 AT1G02220.1 31.293 147 75 6 12 136 3 145 3.15e-12 68.6
Msa1307430 AT3G04420.2 31.126 151 79 6 13 140 4 152 3.36e-12 67.8
Msa1307430 AT5G22290.1 25.781 256 143 11 2 232 19 252 4.35e-12 67.8
Msa1307430 AT3G04420.1 31.126 151 79 6 13 140 4 152 4.92e-12 67.8
Msa1307430 AT2G33480.3 38.372 86 45 3 59 138 20 103 4.99e-12 65.9
Msa1307430 AT1G34180.4 31.902 163 71 5 15 139 19 179 8.22e-12 67.8
Msa1307430 AT1G34180.2 31.288 163 72 5 15 139 19 179 1.79e-11 67.0
Msa1307430 AT1G52880.1 27.751 209 111 8 16 190 21 223 3.55e-11 64.7

Find 102 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGATTCCATATTTATTTC+TGG 0.145339 8_4:-3024201 None:intergenic
ATTGATTCCATATTTATTTC+TGG 0.145339 8_4:-3024658 None:intergenic
AGTACCTTTCGAGATGATTT+TGG 0.182200 8_4:+3025411 Msa1307430:CDS
AGGGGTAGCAATAATATTAT+TGG 0.231520 8_4:+3023241 Msa1307430:CDS
GCATCGTGAATTGGCGAGTT+TGG 0.245634 8_4:-3024745 None:intergenic
AAGTTTAGTTCTAACATATT+AGG 0.259365 8_4:+3025668 Msa1307430:three_prime_UTR
AAATATGGAATCAATATTTC+AGG 0.281347 8_4:+3024209 Msa1307430:CDS
AAATATGGAATCAATATTTC+AGG 0.281347 8_4:+3024666 Msa1307430:CDS
AATGATTATTGACAGGGATT+TGG 0.289338 8_4:+3024386 Msa1307430:CDS
GCATCGTGAAGTGGCGAGTT+TGG 0.289715 8_4:-3024288 None:intergenic
AATGATTATTGACGGGGATT+TGG 0.293193 8_4:+3024843 Msa1307430:CDS
ATAATATCATAGGCTAGTTT+TGG 0.296487 8_4:-3022652 None:intergenic
GATGTTGTCATTTCTAAAAT+AGG 0.297612 8_4:-3024310 None:intergenic
GATGTTGTCATTTCTAAAAT+AGG 0.297612 8_4:-3024767 None:intergenic
TACTATAGTGGTCGTGTTCC+TGG 0.304206 8_4:+3023283 Msa1307430:CDS
AAAACTAAAATTGAGGAATA+AGG 0.314591 8_4:-3025809 None:intergenic
TTTAAAAGGACAACTAATAA+TGG 0.327657 8_4:+3023178 Msa1307430:CDS
CCTTAGGAACAGTTGTAAAA+TGG 0.330273 8_4:+3025755 Msa1307430:three_prime_UTR
GGGGTAGCAATAATATTATT+GGG 0.331309 8_4:+3023242 Msa1307430:CDS
TTCAACTGCAGATATATCTT+TGG 0.332842 8_4:+3025458 Msa1307430:CDS
TGGAAAGGATCGAAAGATTA+AGG 0.337849 8_4:+3023216 Msa1307430:CDS
CTAAAATAGGCATCGTGAAT+TGG 0.340882 8_4:-3024754 None:intergenic
CAAAGAGTAAGAGGTTTAAA+AGG 0.342416 8_4:+3023164 Msa1307430:CDS
ACCACTAAGCTAACACTTCA+TGG 0.352118 8_4:-3025691 None:intergenic
GTCAAGAGTCGGAGGAAATA+AGG 0.354940 8_4:-3022846 None:intergenic
CATCGTGAATTGGCGAGTTT+GGG 0.364030 8_4:-3024744 None:intergenic
TCAACGCATGGTAATGTTCC+TGG 0.366029 8_4:+3025336 Msa1307430:CDS
TACTTTGCCAGAAATAAATA+TGG 0.372071 8_4:+3024194 Msa1307430:CDS
TACTTTGCCAGAAATAAATA+TGG 0.372071 8_4:+3024651 Msa1307430:CDS
CATCGTGAAGTGGCGAGTTT+GGG 0.375477 8_4:-3024287 None:intergenic
TCCTCTTCAATGGTTTCAAA+TGG 0.379573 8_4:-3024345 None:intergenic
TCCTCTTCAATGGTTTCAAA+TGG 0.379573 8_4:-3024802 None:intergenic
GAACTTATCTTCCTCTTCAA+TGG 0.381281 8_4:-3024355 None:intergenic
GGATCGAAAGATTAAGGTTA+GGG 0.381317 8_4:+3023222 Msa1307430:CDS
TTTCTTGAACATCCTAGTTC+AGG 0.382346 8_4:+3025285 Msa1307430:CDS
GATAGAAAACAGGTGCTTCC+AGG 0.383330 8_4:-3025354 None:intergenic
ATAAGTATGCAAAGAGTAAG+AGG 0.388091 8_4:+3023155 Msa1307430:intron
ATGAATCATTTGATCAAGAT+AGG 0.394555 8_4:-3025638 None:intergenic
GAACTTATCTTCCTCTTCAA+TGG 0.404183 8_4:-3024812 None:intergenic
GAGGTTGATCATAATATCAT+AGG 0.407299 8_4:-3022662 None:intergenic
TACTTGCATACATCAACTTC+AGG 0.414938 8_4:-3022973 None:intergenic
AAGACTCTTGTTTACTATAG+TGG 0.415807 8_4:+3023271 Msa1307430:CDS
CCAGTTCGAGTCGTTGAAAA+TGG 0.419694 8_4:+3024422 Msa1307430:CDS
CCAGTCCGAGTCGTTGAAAA+TGG 0.421990 8_4:+3024879 Msa1307430:CDS
GCCATGAAGTGTTAGCTTAG+TGG 0.422146 8_4:+3025690 Msa1307430:three_prime_UTR
TGGCTGGTCTTGGATGATAC+TGG 0.422975 8_4:-3025539 None:intergenic
CGATAGTTACATTCTTAATA+CGG 0.429588 8_4:+3025844 Msa1307430:three_prime_UTR
TTCCTGGTGTTAAAAGCAGC+TGG 0.431255 8_4:+3023299 Msa1307430:CDS
TCATTATTAGTTCAGTTGCT+TGG 0.431816 8_4:-3025610 None:intergenic
AAAGCATGAATTTGTAATGC+TGG 0.434835 8_4:-3022707 None:intergenic
AGAGAGATATGAAGAGATAG+TGG 0.436096 8_4:-3022753 None:intergenic
GACATGAAGATGGCTGGTCT+TGG 0.436307 8_4:-3025549 None:intergenic
CGATATTCTTGATACTTCAA+TGG 0.439525 8_4:+3025260 Msa1307430:CDS
AGGATCGAAAGATTAAGGTT+AGG 0.440394 8_4:+3023221 Msa1307430:CDS
AGACATAGTTCTAATTCCTT+AGG 0.443594 8_4:+3025739 Msa1307430:three_prime_UTR
TCGTTGAAAATGGTCTACTA+TGG 0.447180 8_4:+3024432 Msa1307430:CDS
TCAGTTCAATGATTATTGAC+AGG 0.447967 8_4:+3024379 Msa1307430:CDS
TCGTTGAAAATGGTCTACTA+TGG 0.448952 8_4:+3024889 Msa1307430:CDS
ATTCTTTAAAGATAGAAAAC+AGG 0.461828 8_4:-3025364 None:intergenic
GTCAGTTCAATGATTATTGA+CGG 0.463736 8_4:+3024835 Msa1307430:CDS
ACTCTTGACCATGTTCCTGT+TGG 0.471228 8_4:+3022860 Msa1307430:CDS
CATATTGTGAACTTCTGATA+AGG 0.477364 8_4:+3025867 Msa1307430:three_prime_UTR
TTCAATCTCTGAAAATGAGC+AGG 0.480194 8_4:+3024254 Msa1307430:CDS
TTCAATCTCTGAAAATGAGC+AGG 0.480194 8_4:+3024711 Msa1307430:CDS
TCAGTTCAATGATTATTGAC+GGG 0.480425 8_4:+3024836 Msa1307430:CDS
GATACAGATATATGTGATGA+AGG 0.481936 8_4:+3023986 Msa1307430:CDS
TCGAAAAGAGCTGCTTTAAG+AGG 0.483514 8_4:+3025570 Msa1307430:CDS
GAAATTATCATTAATTAAAG+TGG 0.491758 8_4:-3025787 None:intergenic
GTTACAAGCTCTTCATCTGT+TGG 0.492615 8_4:-3022896 None:intergenic
GCAGAGTATTCGCCTGAACT+AGG 0.495091 8_4:-3025297 None:intergenic
TCGGAACTCATGTGTCTACT+TGG 0.499283 8_4:-3024013 None:intergenic
GGTCTGAATCTGAATCCAAC+AGG 0.502719 8_4:-3022875 None:intergenic
CAGTTCAATGATTATTGACA+GGG 0.506953 8_4:+3024380 Msa1307430:CDS
TCCATTTGAAACCATTGAAG+AGG 0.526651 8_4:+3024344 Msa1307430:CDS
TCCATTTGAAACCATTGAAG+AGG 0.526651 8_4:+3024801 Msa1307430:CDS
ACAAACCTTGTCATCTTCAA+AGG 0.536374 8_4:-3023347 None:intergenic
GGAATCAATATTTCAGGCAC+AGG 0.539733 8_4:+3024215 Msa1307430:CDS
ATACAGATATATGTGATGAA+GGG 0.548662 8_4:+3023987 Msa1307430:CDS
TATGAACCTTGGGAGTTACC+AGG 0.553928 8_4:+3022994 Msa1307430:CDS
CTAAAATAGGCATCGTGAAG+TGG 0.556105 8_4:-3024297 None:intergenic
TGTATGCAAGTATGAACCTT+GGG 0.557609 8_4:+3022984 Msa1307430:CDS
AGGTATAACATTGTTGACAA+TGG 0.563065 8_4:-3022953 None:intergenic
GTGAATTGGCGAGTTTGGGA+TGG 0.564115 8_4:-3024740 None:intergenic
ATTAAGAATGTAACTATCGT+TGG 0.564747 8_4:-3025840 None:intergenic
AATTAGAACTATGTCTCTTG+AGG 0.567105 8_4:-3025732 None:intergenic
CAGTTCAATGATTATTGACG+GGG 0.578711 8_4:+3024837 Msa1307430:CDS
TACAGATATATGTGATGAAG+GGG 0.592526 8_4:+3023988 Msa1307430:CDS
TCCTGGTGTTAAAAGCAGCT+GGG 0.595945 8_4:+3023300 Msa1307430:CDS
ATCTGCAGTTGAATCACAAG+AGG 0.597794 8_4:-3025448 None:intergenic
GTGAAGTGGCGAGTTTGGGA+TGG 0.598328 8_4:-3024283 None:intergenic
TTGAAAATGGTCTACTATGG+AGG 0.604864 8_4:+3024435 Msa1307430:CDS
TACCACCTTTGAAGATGACA+AGG 0.610149 8_4:+3023342 Msa1307430:CDS
ATCTGAATCCAACAGGAACA+TGG 0.616076 8_4:-3022868 None:intergenic
AACCTTGTCATCTTCAAAGG+TGG 0.630537 8_4:-3023344 None:intergenic
ACAGGAACATGGTCAAGAGT+CGG 0.636129 8_4:-3022857 None:intergenic
AAATTGTTGAAATCAACGCA+TGG 0.648209 8_4:+3025324 Msa1307430:CDS
TGCTGAAGTAGTCTCTACGC+CGG 0.650251 8_4:+3024470 Msa1307430:CDS
TGCTGAAGTAGTCTCTACGC+CGG 0.650251 8_4:+3024927 Msa1307430:CDS
GCTGCTTTAAGAGGATCTTG+AGG 0.651591 8_4:+3025579 Msa1307430:CDS
ATGTATGCAAGTATGAACCT+TGG 0.657459 8_4:+3022983 Msa1307430:CDS
GGAACATGGTCAAGAGTCGG+AGG 0.664688 8_4:-3022854 None:intergenic
GATCGAAAGATTAAGGTTAG+GGG 0.678144 8_4:+3023223 Msa1307430:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGTTTTGAAATTTGTTT+TGG - chr8_4:3022795-3022814 None:intergenic 15.0%
!!! TTTTATGAGAAAATAATGTT+GGG - chr8_4:3023044-3023063 None:intergenic 15.0%
!!! TTTTTATGAGAAAATAATGT+TGG - chr8_4:3023045-3023064 None:intergenic 15.0%
!! AAAGATGAAAAACATTAAAA+AGG - chr8_4:3023073-3023092 None:intergenic 15.0%
!!! TTACTATTTTCCTATATTAT+TGG + chr8_4:3023532-3023551 Msa1307430:intron 15.0%
!! ATTTCTATTATCATTTATCA+AGG - chr8_4:3024089-3024108 None:intergenic 15.0%
!!! AGAACTTATATTTGTTTTAT+AGG + chr8_4:3024164-3024183 Msa1307430:intron 15.0%
!! ATTTCTATTATCATTTATCA+AGG - chr8_4:3024089-3024108 None:intergenic 15.0%
!!! AGAACTTATATTTGTTTTAT+AGG + chr8_4:3024164-3024183 Msa1307430:intron 15.0%
!! TTTGAACAATATATATTTAC+CGG - chr8_4:3024949-3024968 None:intergenic 15.0%
!! GAAATTATCATTAATTAAAG+TGG - chr8_4:3025790-3025809 None:intergenic 15.0%
!! AACAAAAACAAAATATAGGA+AGG - chr8_4:3022623-3022642 None:intergenic 20.0%
!!! TTGTTTGTTTTTTTTTTACC+TGG - chr8_4:3023015-3023034 None:intergenic 20.0%
!! TTTATGAGAAAATAATGTTG+GGG - chr8_4:3023043-3023062 None:intergenic 20.0%
!! TTTGCATACTTATAATCAAT+AGG - chr8_4:3023148-3023167 None:intergenic 20.0%
!! TTTAAAAGGACAACTAATAA+TGG + chr8_4:3023178-3023197 Msa1307430:CDS 20.0%
!! TATGATACCAAAAGAAAAAA+GGG - chr8_4:3023613-3023632 None:intergenic 20.0%
!! ATATGATACCAAAAGAAAAA+AGG - chr8_4:3023614-3023633 None:intergenic 20.0%
!!! ACTTATATTTGTTTTATAGG+TGG + chr8_4:3024167-3024186 Msa1307430:intron 20.0%
!!! CTTATATTTGTTTTATAGGT+GGG + chr8_4:3024168-3024187 Msa1307430:intron 20.0%
!!! ATTGATTCCATATTTATTTC+TGG - chr8_4:3024204-3024223 None:intergenic 20.0%
!! AAATATGGAATCAATATTTC+AGG + chr8_4:3024209-3024228 Msa1307430:CDS 20.0%
!!! ACTTATATTTGTTTTATAGG+TGG + chr8_4:3024167-3024186 Msa1307430:intron 20.0%
!!! CTTATATTTGTTTTATAGGT+GGG + chr8_4:3024168-3024187 Msa1307430:intron 20.0%
!!! ATTGATTCCATATTTATTTC+TGG - chr8_4:3024204-3024223 None:intergenic 20.0%
!! AAATATGGAATCAATATTTC+AGG + chr8_4:3024209-3024228 Msa1307430:CDS 20.0%
!!! TATAATTGTGAAAAAGTACT+TGG + chr8_4:3025067-3025086 Msa1307430:intron 20.0%
!! AAGAAAATAAGCATTATGAA+AGG - chr8_4:3025221-3025240 None:intergenic 20.0%
!!! ATTCTTTAAAGATAGAAAAC+AGG - chr8_4:3025367-3025386 None:intergenic 20.0%
!!! TGTTTTCTATCTTTAAAGAA+TGG + chr8_4:3025366-3025385 Msa1307430:CDS 20.0%
!!! AAGTTTAGTTCTAACATATT+AGG + chr8_4:3025668-3025687 Msa1307430:three_prime_UTR 20.0%
!! AAAACTAAAATTGAGGAATA+AGG - chr8_4:3025812-3025831 None:intergenic 20.0%
!! ATGTTCAAAAACTAAAATTG+AGG - chr8_4:3025819-3025838 None:intergenic 20.0%
! GCTGAACAAAAACAAAATAT+AGG - chr8_4:3022627-3022646 None:intergenic 25.0%
!!! ATAATATCATAGGCTAGTTT+TGG - chr8_4:3022655-3022674 None:intergenic 25.0%
!!! GGTTTGTTTTGTTACAATAA+AGG + chr8_4:3023363-3023382 Msa1307430:intron 25.0%
!! TTTGGTATCATATTTGTTAG+AGG + chr8_4:3023621-3023640 Msa1307430:intron 25.0%
! AAAACACAAATCACAAACAA+TGG + chr8_4:3023669-3023688 Msa1307430:intron 25.0%
! ACACTGTCTTTGTAATTAAA+AGG - chr8_4:3023702-3023721 None:intergenic 25.0%
! ATACAGATATATGTGATGAA+GGG + chr8_4:3023987-3024006 Msa1307430:CDS 25.0%
!! CAAAAGTTTCTTGATTTTCA+TGG - chr8_4:3024039-3024058 None:intergenic 25.0%
!! GAAAATCAAGAAACTTTTGA+AGG + chr8_4:3024040-3024059 Msa1307430:CDS 25.0%
!! AAATTTTGCCAACAAAGTTT+TGG + chr8_4:3024105-3024124 Msa1307430:intron 25.0%
! TACTTTGCCAGAAATAAATA+TGG + chr8_4:3024194-3024213 Msa1307430:CDS 25.0%
!!! GATGTTGTCATTTCTAAAAT+AGG - chr8_4:3024313-3024332 None:intergenic 25.0%
! CAGGAACAATATATATTTAC+CGG - chr8_4:3024492-3024511 None:intergenic 25.0%
! TATATATTGTTCCTGATGTA+AGG + chr8_4:3024497-3024516 Msa1307430:intron 25.0%
!! AAATTTTGCCAACAAAGTTT+TGG + chr8_4:3024105-3024124 Msa1307430:intron 25.0%
! TACTTTGCCAGAAATAAATA+TGG + chr8_4:3024194-3024213 Msa1307430:CDS 25.0%
!!! GATGTTGTCATTTCTAAAAT+AGG - chr8_4:3024313-3024332 None:intergenic 25.0%
! ATACACACTTTCATATCATT+TGG - chr8_4:3024974-3024993 None:intergenic 25.0%
! AAAGTGTGTATCTCTTATTA+GGG + chr8_4:3024983-3025002 Msa1307430:intron 25.0%
! ATGAATCATTTGATCAAGAT+AGG - chr8_4:3025641-3025660 None:intergenic 25.0%
! ATTAAGAATGTAACTATCGT+TGG - chr8_4:3025843-3025862 None:intergenic 25.0%
! CGATAGTTACATTCTTAATA+CGG + chr8_4:3025844-3025863 Msa1307430:three_prime_UTR 25.0%
! GAGGTTGATCATAATATCAT+AGG - chr8_4:3022665-3022684 None:intergenic 30.0%
AAAGCATGAATTTGTAATGC+TGG - chr8_4:3022710-3022729 None:intergenic 30.0%
!!! GTAACTTGTTTTTGAGATAG+TGG - chr8_4:3022921-3022940 None:intergenic 30.0%
AGGTATAACATTGTTGACAA+TGG - chr8_4:3022956-3022975 None:intergenic 30.0%
!!! TTCATCTTTTTCAGCTTGTT+CGG + chr8_4:3023084-3023103 Msa1307430:intron 30.0%
ATAAGTATGCAAAGAGTAAG+AGG + chr8_4:3023155-3023174 Msa1307430:intron 30.0%
CAAAGAGTAAGAGGTTTAAA+AGG + chr8_4:3023164-3023183 Msa1307430:CDS 30.0%
! GGACAACTAATAATGGTTTT+TGG + chr8_4:3023185-3023204 Msa1307430:CDS 30.0%
AGGGGTAGCAATAATATTAT+TGG + chr8_4:3023241-3023260 Msa1307430:CDS 30.0%
GGGGTAGCAATAATATTATT+GGG + chr8_4:3023242-3023261 Msa1307430:CDS 30.0%
! AAGACTCTTGTTTACTATAG+TGG + chr8_4:3023271-3023290 Msa1307430:CDS 30.0%
GACAAAACATGAGTTTATTC+AGG + chr8_4:3023507-3023526 Msa1307430:intron 30.0%
TTCAGAACAGCCAATAATAT+AGG - chr8_4:3023545-3023564 None:intergenic 30.0%
!!! CTTTGAACCCTTTTTTCTTT+TGG + chr8_4:3023603-3023622 Msa1307430:intron 30.0%
AAAAGACTAAAAGAGTGACT+TGG - chr8_4:3023774-3023793 None:intergenic 30.0%
! TATCATCCGAAATTTTGTAG+CGG + chr8_4:3023906-3023925 Msa1307430:intron 30.0%
AAAAGTCCGCTACAAAATTT+CGG - chr8_4:3023915-3023934 None:intergenic 30.0%
GATACAGATATATGTGATGA+AGG + chr8_4:3023986-3024005 Msa1307430:CDS 30.0%
TACAGATATATGTGATGAAG+GGG + chr8_4:3023988-3024007 Msa1307430:CDS 30.0%
! AGTTTCTTGATTTTCATGGT+CGG - chr8_4:3024035-3024054 None:intergenic 30.0%
!! CTTTAAAGCCAAAACTTTGT+TGG - chr8_4:3024116-3024135 None:intergenic 30.0%
TCAGTTCAATGATTATTGAC+AGG + chr8_4:3024379-3024398 Msa1307430:CDS 30.0%
CAGTTCAATGATTATTGACA+GGG + chr8_4:3024380-3024399 Msa1307430:CDS 30.0%
AATGATTATTGACAGGGATT+TGG + chr8_4:3024386-3024405 Msa1307430:CDS 30.0%
!! CTTTAAAGCCAAAACTTTGT+TGG - chr8_4:3024116-3024135 None:intergenic 30.0%
GTCAGTTCAATGATTATTGA+CGG + chr8_4:3024835-3024854 Msa1307430:CDS 30.0%
TCAGTTCAATGATTATTGAC+GGG + chr8_4:3024379-3024398 Msa1307430:CDS 30.0%
! GAAAGTGTGTATCTCTTATT+AGG + chr8_4:3024982-3025001 Msa1307430:intron 30.0%
TTGGATGAAATGAAATGTCT+AGG + chr8_4:3025086-3025105 Msa1307430:intron 30.0%
CATTCTAGTAGCATTTCAAA+AGG - chr8_4:3025197-3025216 None:intergenic 30.0%
! TCTTCTACAATTTTACAGTG+TGG + chr8_4:3025237-3025256 Msa1307430:intron 30.0%
CGATATTCTTGATACTTCAA+TGG + chr8_4:3025260-3025279 Msa1307430:CDS 30.0%
AAATTGTTGAAATCAACGCA+TGG + chr8_4:3025324-3025343 Msa1307430:CDS 30.0%
TTCAACTGCAGATATATCTT+TGG + chr8_4:3025458-3025477 Msa1307430:CDS 30.0%
! GTTTTTGCGTTTTCTTATAG+TGG - chr8_4:3025520-3025539 None:intergenic 30.0%
TCATTATTAGTTCAGTTGCT+TGG - chr8_4:3025613-3025632 None:intergenic 30.0%
AATTAGAACTATGTCTCTTG+AGG - chr8_4:3025735-3025754 None:intergenic 30.0%
!! AGACATAGTTCTAATTCCTT+AGG + chr8_4:3025739-3025758 Msa1307430:three_prime_UTR 30.0%
CATATTGTGAACTTCTGATA+AGG + chr8_4:3025867-3025886 Msa1307430:three_prime_UTR 30.0%
AGAGAGATATGAAGAGATAG+TGG - chr8_4:3022756-3022775 None:intergenic 35.0%
TACTTGCATACATCAACTTC+AGG - chr8_4:3022976-3022995 None:intergenic 35.0%
ATGTATGCAAGTATGAACCT+TGG + chr8_4:3022983-3023002 Msa1307430:CDS 35.0%
TGTATGCAAGTATGAACCTT+GGG + chr8_4:3022984-3023003 Msa1307430:CDS 35.0%
TAAAATCAGATGACCCAGAA+TGG + chr8_4:3023110-3023129 Msa1307430:intron 35.0%
GACACAAGAAAAACCATTCT+GGG - chr8_4:3023126-3023145 None:intergenic 35.0%
!! AATGGTTTTTGGAAAGCTAC+TGG + chr8_4:3023196-3023215 Msa1307430:CDS 35.0%
!! TTTTTGGAAAGCTACTGGAA+AGG + chr8_4:3023201-3023220 Msa1307430:CDS 35.0%
TGGAAAGGATCGAAAGATTA+AGG + chr8_4:3023216-3023235 Msa1307430:CDS 35.0%
AGGATCGAAAGATTAAGGTT+AGG + chr8_4:3023221-3023240 Msa1307430:CDS 35.0%
GGATCGAAAGATTAAGGTTA+GGG + chr8_4:3023222-3023241 Msa1307430:CDS 35.0%
GATCGAAAGATTAAGGTTAG+GGG + chr8_4:3023223-3023242 Msa1307430:CDS 35.0%
ACAAACCTTGTCATCTTCAA+AGG - chr8_4:3023350-3023369 None:intergenic 35.0%
!! ACTAAAAGAGTGACTTGGTA+AGG - chr8_4:3023769-3023788 None:intergenic 35.0%
!!! CGCATTAGTTTGTTTTGTCT+TGG + chr8_4:3023846-3023865 Msa1307430:intron 35.0%
AGCTGAGAAAAAAGCTGAAA+AGG + chr8_4:3023958-3023977 Msa1307430:CDS 35.0%
TTGAAGGAATTCCTGATGTA+AGG + chr8_4:3024056-3024075 Msa1307430:intron 35.0%
TCAGCATGTTTCATGTAGAA+AGG - chr8_4:3024145-3024164 None:intergenic 35.0%
! CATTTTCAGAGATTGAAGGA+AGG - chr8_4:3024251-3024270 None:intergenic 35.0%
! TGCTCATTTTCAGAGATTGA+AGG - chr8_4:3024255-3024274 None:intergenic 35.0%
! TTCAATCTCTGAAAATGAGC+AGG + chr8_4:3024254-3024273 Msa1307430:CDS 35.0%
! TCCATTTGAAACCATTGAAG+AGG + chr8_4:3024344-3024363 Msa1307430:CDS 35.0%
TCCTCTTCAATGGTTTCAAA+TGG - chr8_4:3024348-3024367 None:intergenic 35.0%
GAACTTATCTTCCTCTTCAA+TGG - chr8_4:3024358-3024377 None:intergenic 35.0%
TCGTTGAAAATGGTCTACTA+TGG + chr8_4:3024432-3024451 Msa1307430:CDS 35.0%
TTGAAAATGGTCTACTATGG+AGG + chr8_4:3024435-3024454 Msa1307430:CDS 35.0%
TCAGCATGTTTCATGTAGAA+AGG - chr8_4:3024145-3024164 None:intergenic 35.0%
! CATTTTCAGAGATTGAAGGA+AGG - chr8_4:3024251-3024270 None:intergenic 35.0%
! TGCTCATTTTCAGAGATTGA+AGG - chr8_4:3024255-3024274 None:intergenic 35.0%
! TTCAATCTCTGAAAATGAGC+AGG + chr8_4:3024254-3024273 Msa1307430:CDS 35.0%
! CTAAAATAGGCATCGTGAAT+TGG - chr8_4:3024757-3024776 None:intergenic 35.0%
! TCCATTTGAAACCATTGAAG+AGG + chr8_4:3024344-3024363 Msa1307430:CDS 35.0%
TCCTCTTCAATGGTTTCAAA+TGG - chr8_4:3024348-3024367 None:intergenic 35.0%
GAACTTATCTTCCTCTTCAA+TGG - chr8_4:3024358-3024377 None:intergenic 35.0%
CAGTTCAATGATTATTGACG+GGG + chr8_4:3024837-3024856 Msa1307430:CDS 35.0%
AATGATTATTGACGGGGATT+TGG + chr8_4:3024843-3024862 Msa1307430:CDS 35.0%
TCGTTGAAAATGGTCTACTA+TGG + chr8_4:3024432-3024451 Msa1307430:CDS 35.0%
TTTCTTGAACATCCTAGTTC+AGG + chr8_4:3025285-3025304 Msa1307430:CDS 35.0%
! AGTACCTTTCGAGATGATTT+TGG + chr8_4:3025411-3025430 Msa1307430:CDS 35.0%
! GTACCTTTCGAGATGATTTT+GGG + chr8_4:3025412-3025431 Msa1307430:CDS 35.0%
! TACCTTTCGAGATGATTTTG+GGG + chr8_4:3025413-3025432 Msa1307430:CDS 35.0%
!! TTGCGTTTTCTTATAGTGGA+AGG - chr8_4:3025516-3025535 None:intergenic 35.0%
! CCATTTTACAACTGTTCCTA+AGG - chr8_4:3025758-3025777 None:intergenic 35.0%
CCTTAGGAACAGTTGTAAAA+TGG + chr8_4:3025755-3025774 Msa1307430:three_prime_UTR 35.0%
! TAGTGTGTGTGAGAGTTTTG+AGG - chr8_4:3022684-3022703 None:intergenic 40.0%
ATCTGAATCCAACAGGAACA+TGG - chr8_4:3022871-3022890 None:intergenic 40.0%
GTTACAAGCTCTTCATCTGT+TGG - chr8_4:3022899-3022918 None:intergenic 40.0%
! TTTTTACCTGGTAACTCCCA+AGG - chr8_4:3023003-3023022 None:intergenic 40.0%
!!! ATCTTTTTCAGCTTGTTCGG+TGG + chr8_4:3023087-3023106 Msa1307430:intron 40.0%
GGACACAAGAAAAACCATTC+TGG - chr8_4:3023127-3023146 None:intergenic 40.0%
! TACCACCTTTGAAGATGACA+AGG + chr8_4:3023342-3023361 Msa1307430:CDS 40.0%
AACCTTGTCATCTTCAAAGG+TGG - chr8_4:3023347-3023366 None:intergenic 40.0%
AAGGATGAACTCCTTACATC+AGG - chr8_4:3024070-3024089 None:intergenic 40.0%
GGAATCAATATTTCAGGCAC+AGG + chr8_4:3024215-3024234 Msa1307430:CDS 40.0%
! CTAAAATAGGCATCGTGAAG+TGG - chr8_4:3024300-3024319 None:intergenic 40.0%
TGTAAGGAGTTCCTGATGTA+AGG + chr8_4:3024513-3024532 Msa1307430:intron 40.0%
AAGGATGAACTCCTTACATC+AGG - chr8_4:3024070-3024089 None:intergenic 40.0%
! GTAGACCATTTTCAACGACT+CGG - chr8_4:3024430-3024449 None:intergenic 40.0%
TATTAGGGAACCATCCATTG+CGG + chr8_4:3024998-3025017 Msa1307430:intron 40.0%
!! GACCACATTTTAGCAATGCT+TGG + chr8_4:3025033-3025052 Msa1307430:intron 40.0%
! CACCAAGCATTGCTAAAATG+TGG - chr8_4:3025038-3025057 None:intergenic 40.0%
! ACCTTTCGAGATGATTTTGG+GGG + chr8_4:3025414-3025433 Msa1307430:CDS 40.0%
!! AGATGATTTTGGGGGATTAG+AGG + chr8_4:3025422-3025441 Msa1307430:CDS 40.0%
ATCTGCAGTTGAATCACAAG+AGG - chr8_4:3025451-3025470 None:intergenic 40.0%
! AGCTCTTTTCGACATGAAGA+TGG - chr8_4:3025562-3025581 None:intergenic 40.0%
! TCGAAAAGAGCTGCTTTAAG+AGG + chr8_4:3025570-3025589 Msa1307430:CDS 40.0%
ACCACTAAGCTAACACTTCA+TGG - chr8_4:3025694-3025713 None:intergenic 40.0%
GTCAAGAGTCGGAGGAAATA+AGG - chr8_4:3022849-3022868 None:intergenic 45.0%
ACAGGAACATGGTCAAGAGT+CGG - chr8_4:3022860-3022879 None:intergenic 45.0%
ACTCTTGACCATGTTCCTGT+TGG + chr8_4:3022860-3022879 Msa1307430:CDS 45.0%
GGTCTGAATCTGAATCCAAC+AGG - chr8_4:3022878-3022897 None:intergenic 45.0%
TATGAACCTTGGGAGTTACC+AGG + chr8_4:3022994-3023013 Msa1307430:CDS 45.0%
TACTATAGTGGTCGTGTTCC+TGG + chr8_4:3023283-3023302 Msa1307430:CDS 45.0%
! TTCCTGGTGTTAAAAGCAGC+TGG + chr8_4:3023299-3023318 Msa1307430:CDS 45.0%
! TCCTGGTGTTAAAAGCAGCT+GGG + chr8_4:3023300-3023319 Msa1307430:CDS 45.0%
TCGGAACTCATGTGTCTACT+TGG - chr8_4:3024016-3024035 None:intergenic 45.0%
! CCATTTTCAACGACTCGAAC+TGG - chr8_4:3024425-3024444 None:intergenic 45.0%
CCAGTTCGAGTCGTTGAAAA+TGG + chr8_4:3024422-3024441 Msa1307430:CDS 45.0%
CATCAGGAACTCCTTACATC+AGG - chr8_4:3024511-3024530 None:intergenic 45.0%
CATCGTGAATTGGCGAGTTT+GGG - chr8_4:3024747-3024766 None:intergenic 45.0%
TCAAACTCATGTCACCGCAA+TGG - chr8_4:3025015-3025034 None:intergenic 45.0%
! TCAACGCATGGTAATGTTCC+TGG + chr8_4:3025336-3025355 Msa1307430:CDS 45.0%
GATAGAAAACAGGTGCTTCC+AGG - chr8_4:3025357-3025376 None:intergenic 45.0%
TCCCCCAAAATCATCTCGAA+AGG - chr8_4:3025418-3025437 None:intergenic 45.0%
! CTTTTCGACATGAAGATGGC+TGG - chr8_4:3025558-3025577 None:intergenic 45.0%
!! GCTGCTTTAAGAGGATCTTG+AGG + chr8_4:3025579-3025598 Msa1307430:CDS 45.0%
! GCCATGAAGTGTTAGCTTAG+TGG + chr8_4:3025690-3025709 Msa1307430:three_prime_UTR 45.0%
!!! ACCCAGCTGCTTTTAACACC+AGG - chr8_4:3023304-3023323 None:intergenic 50.0%
CATCGTGAAGTGGCGAGTTT+GGG - chr8_4:3024290-3024309 None:intergenic 50.0%
TGCTGAAGTAGTCTCTACGC+CGG + chr8_4:3024470-3024489 Msa1307430:CDS 50.0%
GTGAATTGGCGAGTTTGGGA+TGG - chr8_4:3024743-3024762 None:intergenic 50.0%
GCATCGTGAATTGGCGAGTT+TGG - chr8_4:3024748-3024767 None:intergenic 50.0%
!! CCATTTTCAACGACTCGGAC+TGG - chr8_4:3024882-3024901 None:intergenic 50.0%
CCAGTCCGAGTCGTTGAAAA+TGG + chr8_4:3024879-3024898 Msa1307430:CDS 50.0%
TGCTGAAGTAGTCTCTACGC+CGG + chr8_4:3024470-3024489 Msa1307430:CDS 50.0%
ACTCATGTCACCGCAATGGA+TGG - chr8_4:3025011-3025030 None:intergenic 50.0%
! GCAGAGTATTCGCCTGAACT+AGG - chr8_4:3025300-3025319 None:intergenic 50.0%
!! TGGCTGGTCTTGGATGATAC+TGG - chr8_4:3025542-3025561 None:intergenic 50.0%
!! GACATGAAGATGGCTGGTCT+TGG - chr8_4:3025552-3025571 None:intergenic 50.0%
GGAACATGGTCAAGAGTCGG+AGG - chr8_4:3022857-3022876 None:intergenic 55.0%
GTGAAGTGGCGAGTTTGGGA+TGG - chr8_4:3024286-3024305 None:intergenic 55.0%
GCATCGTGAAGTGGCGAGTT+TGG - chr8_4:3024291-3024310 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 3022612 3025974 3022612 ID=Msa1307430;Name=Msa1307430
chr8_4 mRNA 3022612 3025974 3022612 ID=Msa1307430-mRNA-1;Parent=Msa1307430;Name=Msa1307430-mRNA-1;_AED=0.10;_eAED=0.10;_QI=227|0.57|0.37|1|0.71|0.5|8|375|490
chr8_4 exon 3022612 3023015 3022612 ID=Msa1307430-mRNA-1:exon:901;Parent=Msa1307430-mRNA-1
chr8_4 exon 3023160 3023363 3023160 ID=Msa1307430-mRNA-1:exon:902;Parent=Msa1307430-mRNA-1
chr8_4 exon 3023926 3024072 3023926 ID=Msa1307430-mRNA-1:exon:903;Parent=Msa1307430-mRNA-1
chr8_4 exon 3024186 3024335 3024186 ID=Msa1307430-mRNA-1:exon:904;Parent=Msa1307430-mRNA-1
chr8_4 exon 3024342 3024491 3024342 ID=Msa1307430-mRNA-1:exon:905;Parent=Msa1307430-mRNA-1
chr8_4 exon 3024643 3024792 3024643 ID=Msa1307430-mRNA-1:exon:906;Parent=Msa1307430-mRNA-1
chr8_4 exon 3024799 3024948 3024799 ID=Msa1307430-mRNA-1:exon:907;Parent=Msa1307430-mRNA-1
chr8_4 exon 3025255 3025974 3025255 ID=Msa1307430-mRNA-1:exon:908;Parent=Msa1307430-mRNA-1
chr8_4 five_prime_UTR 3022612 3022838 3022612 ID=Msa1307430-mRNA-1:five_prime_utr;Parent=Msa1307430-mRNA-1
chr8_4 CDS 3022839 3023015 3022839 ID=Msa1307430-mRNA-1:cds;Parent=Msa1307430-mRNA-1
chr8_4 CDS 3023160 3023363 3023160 ID=Msa1307430-mRNA-1:cds;Parent=Msa1307430-mRNA-1
chr8_4 CDS 3023926 3024072 3023926 ID=Msa1307430-mRNA-1:cds;Parent=Msa1307430-mRNA-1
chr8_4 CDS 3024186 3024335 3024186 ID=Msa1307430-mRNA-1:cds;Parent=Msa1307430-mRNA-1
chr8_4 CDS 3024342 3024491 3024342 ID=Msa1307430-mRNA-1:cds;Parent=Msa1307430-mRNA-1
chr8_4 CDS 3024643 3024792 3024643 ID=Msa1307430-mRNA-1:cds;Parent=Msa1307430-mRNA-1
chr8_4 CDS 3024799 3024948 3024799 ID=Msa1307430-mRNA-1:cds;Parent=Msa1307430-mRNA-1
chr8_4 CDS 3025255 3025599 3025255 ID=Msa1307430-mRNA-1:cds;Parent=Msa1307430-mRNA-1
chr8_4 three_prime_UTR 3025600 3025974 3025600 ID=Msa1307430-mRNA-1:three_prime_utr;Parent=Msa1307430-mRNA-1
Gene Sequence

>Msa1307430

ATGACAACCTTATTTCCTCCGACTCTTGACCATGTTCCTGTTGGATTCAGATTCAGACCAACAGATGAAGAGCTTGTAACCACTATCTCAAAAACAAGTTACTTGATAACGATTCCATTGTCAACAATGTTATACCTGAAGTTGATGTATGCAAGTATGAACCTTGGGAGTTACCAGTATGCAAAGAGTAAGAGGTTTAAAAGGACAACTAATAATGGTTTTTGGAAAGCTACTGGAAAGGATCGAAAGATTAAGGTTAGGGGTAGCAATAATATTATTGGGATTAAGAAGACTCTTGTTTACTATAGTGGTCGTGTTCCTGGTGTTAAAAGCAGCTGGGTGATTCATGAATATCATGATACCACCTTTGAAGATGACAAGCGGACTTTTGTTTTATGTCGCTTGATGAAGAAAGCTGAGAAAAAAGCTGAAAAGGAAGCTGATACAGATATATGTGATGAAGGGGAGCCAAGTAGACACATGAGTTCCGACCATGAAAATCAAGAAACTTTTGAAGGAATTCCTGATGTGGGTGATACTTTGCCAGAAATAAATATGGAATCAATATTTCAGGCACAGGCAGAAAAATACCTTCCTTCAATCTCTGAAAATGAGCAGGAAGTATCCATCCCAAACTCGCCACTTCACGATGCCTATTTTAGAAATGACAACATCATTAGTCCATTTGAAACCATTGAAGAGGAAGATAAGTTCATCAGTTCAATGATTATTGACAGGGATTTGGACAACGCTCATGTCCAGTTCGAGTCGTTGAAAATGGTCTACTATGGAGGCAGTGACACAGATGCTGAAGTAGTCTCTACGCCGGTGGGTGATACTTTGCCAGAAATAAATATGGAATCAATATTTCAGGCACACGCTGAAAAATACCTTCCTTCAATCTCTGAAAATGAGCAGGAAGTATCCATCCCAAACTCGCCAATTCACGATGCCTATTTTAGAAATGACAACATCATTAGTCCATTTGAAACCATTGAAGAGGAAGATAAGTTCGTCAGTTCAATGATTATTGACGGGGATTTGGACAACGCTCATGTCCAGTCCGAGTCGTTGAAAATGGTCTACTATGGAAGCAGTGACACAGATGCTGAAGTAGTCTCTACGCCGTGTGGCGATATTCTTGATACTTCAATGGCATTTCTTGAACATCCTAGTTCAGGCGAATACTCTGCATCAAAATTGTTGAAATCAACGCATGGTAATGTTCCTGGAAGCACCTGTTTTCTATCTTTAAAGAATGGTGAAATCGAAGACAACAACGAGAGTACCTTTCGAGATGATTTTGGGGGATTAGAGGCATCCTCTTGTGATTCAACTGCAGATATATCTTTGGAGACGATCAATTGTATTGAAATTTCTAGTTTACCTTCCACTATAAGAAAACGCAAAAACCAGTATCATCCAAGACCAGCCATCTTCATGTCGAAAAGAGCTGCTTTAAGAGGATCTTGA
Protein sequence

>Msa1307430

MTTLFPPTLDHVPVGFRFRPTDEELVTTISKTSYLITIPLSTMLYLKLMYASMNLGSYQYAKSKRFKRTTNNGFWKATGKDRKIKVRGSNNIIGIKKTLVYYSGRVPGVKSSWVIHEYHDTTFEDDKRTFVLCRLMKKAEKKAEKEADTDICDEGEPSRHMSSDHENQETFEGIPDVGDTLPEINMESIFQAQAEKYLPSISENEQEVSIPNSPLHDAYFRNDNIISPFETIEEEDKFISSMIIDRDLDNAHVQFESLKMVYYGGSDTDAEVVSTPVGDTLPEINMESIFQAHAEKYLPSISENEQEVSIPNSPIHDAYFRNDNIISPFETIEEEDKFVSSMIIDGDLDNAHVQSESLKMVYYGSSDTDAEVVSTPCGDILDTSMAFLEHPSSGEYSASKLLKSTHGNVPGSTCFLSLKNGEIEDNNESTFRDDFGGLEASSCDSTADISLETINCIEISSLPSTIRKRKNQYHPRPAIFMSKRAALRGS*