AlfalfaGEDB | Msa1313330

M. sativa cultivar zhongmu-4 / Msa1313330

KEGG: K09589|K12638
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004497 GO:0006629 GO:0006694 GO:0007275 GO:0008150 GO:0008152 GO:0008202 GO:0008610 GO:0009058 GO:0009791 GO:0009908 GO:0010268 GO:0010817 GO:0016125 GO:0016128 GO:0016129 GO:0016131 GO:0016132 GO:0016491 GO:0016705 GO:0016709 GO:0022414 GO:0032501 GO:0032502 GO:0042445 GO:0042446 GO:0042592 GO:0044238 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048441 GO:0048443 GO:0048465 GO:0048466 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048878 GO:0055088 GO:0055114 GO:0061458 GO:0065007 GO:0065008 GO:0071704 GO:0090567 GO:0099402 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617
PFAM: PF00067.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1313330	XP_013444311.1	94.130	494	8	2	1	494	1	473	0.0	951

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1313330	sp\|Q94IA6\|C90D1_ARATH	64.363	463	139	4	36	493	50	491	0.0	619

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1313330	A0A072TNC8	94.130	494	8	2	1	494	1	473	0.0	951

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0744640	Msa1313330	0.803804	2.926121e-49	-8.615850e-47
Msa0841730	Msa1313330	0.807496	4.912843e-50	-8.615850e-47
Msa1232750	Msa1313330	0.880624	4.708947e-70	-8.615850e-47
Msa1238600	Msa1313330	0.803101	4.092904e-49	-8.615850e-47
Msa1275090	Msa1313330	0.876743	1.095070e-68	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1313330	MtrunA17_Chr8g0341951	94.130	494	8	2	1	494	1	473	0.0	951
Msa1313330	MtrunA17_Chr8g0372411	52.700	463	192	6	38	495	40	480	4.81e-175	501
Msa1313330	MtrunA17_Chr3g0113321	38.745	462	264	7	34	494	26	469	1.32e-114	347
Msa1313330	MtrunA17_Chr5g0403931	34.400	500	292	9	2	491	5	478	2.15e-99	307
Msa1313330	MtrunA17_Chr6g0478161	32.091	483	309	7	1	482	15	479	5.36e-96	299
Msa1313330	MtrunA17_Chr8g0373441	37.831	415	238	8	8	414	10	412	8.68e-93	288
Msa1313330	MtrunA17_Chr7g0226771	32.371	485	294	9	3	483	6	460	7.59e-92	288
Msa1313330	MtrunA17_Chr6g0455461	34.649	456	259	11	33	481	29	452	1.40e-90	284
Msa1313330	MtrunA17_Chr3g0078411	34.430	456	260	11	33	481	29	452	4.69e-90	283
Msa1313330	MtrunA17_Chr7g0219961	34.286	455	259	11	33	480	29	450	1.54e-88	278

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1313330	AT3G13730.1	64.363	463	139	4	36	493	50	491	0.0	619
Msa1313330	AT3G13730.2	64.450	436	129	4	36	466	50	464	0.0	578
Msa1313330	AT4G36380.1	54.212	463	189	6	37	494	69	513	1.65e-180	516
Msa1313330	AT4G36380.2	50.549	364	164	5	37	395	69	421	4.94e-123	366
Msa1313330	AT5G05690.1	42.048	459	241	7	37	492	31	467	3.19e-121	363
Msa1313330	AT3G50660.1	35.451	488	280	7	32	493	34	512	6.72e-103	318
Msa1313330	AT1G73340.1	33.333	486	298	9	13	495	20	482	6.00e-98	304
Msa1313330	AT5G05690.2	40.970	371	214	4	37	407	31	396	3.43e-95	294
Msa1313330	AT5G38970.1	34.658	453	259	9	34	480	31	452	1.44e-88	279
Msa1313330	AT5G05690.3	40.541	370	195	7	126	492	1	348	3.86e-87	272

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Find 106 sgRNAs with CRISPR-Local

Find 280 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGATAAATGGAGGGCC+AGG	0.474075	8_4:-11221801	Msa1313330:three_prime_UTR
AAAAGTAAATACCTAATTCA+CGG	0.515492	8_4:+11221665	None:intergenic
AAACTCAAAGATCAGCTTGG+GGG	0.657592	8_4:-11223328	Msa1313330:CDS
AAAGGGTATTTAATACCACA+AGG	0.700611	8_4:-11223099	Msa1313330:CDS
AAAGTAAATACCTAATTCAC+GGG	0.496900	8_4:+11221666	None:intergenic
AAATCATTGATGCAGTTAAT+GGG	0.450113	8_4:-11225402	Msa1313330:CDS
AACAAGGTGATGCAGAAAGA+TGG	0.521247	8_4:+11222602	None:intergenic
AACCTTGCCAAGTCAAGACC+AGG	0.613082	8_4:+11222633	None:intergenic
AACTCAAAGATCAGCTTGGG+GGG	0.566190	8_4:-11223327	Msa1313330:CDS
AACTTACATGGCGCATCGCT+TGG	0.445087	8_4:+11225791	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAACAATGGACACTACT+TGG	-	chr8_4:11221623-11221642	Msa1313330:three_prime_UTR	30.0%
AAAAACAATGGACACTACTT+GGG	-	chr8_4:11221624-11221643	Msa1313330:three_prime_UTR	30.0%
ATCAAAGTGCACAAGAAAAT+TGG	+	chr8_4:11221664-11221683	None:intergenic	30.0%
AGCACAAGAAACAAAATCTA+TGG	+	chr8_4:11221782-11221801	None:intergenic	30.0%
CAAAAACAAACAATCTTGCA+CGG	+	chr8_4:11222044-11222063	None:intergenic	30.0%
TATACTACAAAGTGATGCAA+AGG	-	chr8_4:11222176-11222195	Msa1313330:intron	30.0%
CATTAACTGCATCAATGATT+TGG	+	chr8_4:11222220-11222239	None:intergenic	30.0%
CAAATCATTGATGCAGTTAA+TGG	-	chr8_4:11222218-11222237	Msa1313330:intron	30.0%
AGAAGAATACATGGACTTAT+TGG	-	chr8_4:11222279-11222298	Msa1313330:intron	30.0%
GAAGTATGTACAAGAATCAA+TGG	-	chr8_4:11222353-11222372	Msa1313330:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	11221572	11226071	11221572	ID=Msa1313330;Name=Msa1313330
chr8_4	mRNA	11221572	11226071	11221572	ID=Msa1313330-mRNA-1;Parent=Msa1313330;Name=Msa1313330-mRNA-1;_AED=0.08;_eAED=0.08;_QI=59\|0.62\|0.77\|0.88\|1\|1\|9\|325\|495
chr8_4	exon	11221572	11221987	11221572	ID=Msa1313330-mRNA-1:exon:4237;Parent=Msa1313330-mRNA-1
chr8_4	exon	11222591	11222712	11222591	ID=Msa1313330-mRNA-1:exon:4236;Parent=Msa1313330-mRNA-1
chr8_4	exon	11222868	11222921	11222868	ID=Msa1313330-mRNA-1:exon:4235;Parent=Msa1313330-mRNA-1
chr8_4	exon	11223011	11223196	11223011	ID=Msa1313330-mRNA-1:exon:4234;Parent=Msa1313330-mRNA-1
chr8_4	exon	11223282	11223371	11223282	ID=Msa1313330-mRNA-1:exon:4233;Parent=Msa1313330-mRNA-1
chr8_4	exon	11223998	11224243	11223998	ID=Msa1313330-mRNA-1:exon:4232;Parent=Msa1313330-mRNA-1
chr8_4	exon	11224320	11224478	11224320	ID=Msa1313330-mRNA-1:exon:4231;Parent=Msa1313330-mRNA-1
chr8_4	exon	11225218	11225542	11225218	ID=Msa1313330-mRNA-1:exon:4230;Parent=Msa1313330-mRNA-1
chr8_4	exon	11225798	11226071	11225798	ID=Msa1313330-mRNA-1:exon:4229;Parent=Msa1313330-mRNA-1
chr8_4	five_prime_UTR	11226013	11226071	11226013	ID=Msa1313330-mRNA-1:five_prime_utr;Parent=Msa1313330-mRNA-1
chr8_4	CDS	11225798	11226012	11225798	ID=Msa1313330-mRNA-1:cds;Parent=Msa1313330-mRNA-1
chr8_4	CDS	11225218	11225542	11225218	ID=Msa1313330-mRNA-1:cds;Parent=Msa1313330-mRNA-1
chr8_4	CDS	11224320	11224478	11224320	ID=Msa1313330-mRNA-1:cds;Parent=Msa1313330-mRNA-1
chr8_4	CDS	11223998	11224243	11223998	ID=Msa1313330-mRNA-1:cds;Parent=Msa1313330-mRNA-1
chr8_4	CDS	11223282	11223371	11223282	ID=Msa1313330-mRNA-1:cds;Parent=Msa1313330-mRNA-1
chr8_4	CDS	11223011	11223196	11223011	ID=Msa1313330-mRNA-1:cds;Parent=Msa1313330-mRNA-1
chr8_4	CDS	11222868	11222921	11222868	ID=Msa1313330-mRNA-1:cds;Parent=Msa1313330-mRNA-1
chr8_4	CDS	11222591	11222712	11222591	ID=Msa1313330-mRNA-1:cds;Parent=Msa1313330-mRNA-1
chr8_4	CDS	11221897	11221987	11221897	ID=Msa1313330-mRNA-1:cds;Parent=Msa1313330-mRNA-1
chr8_4	three_prime_UTR	11221572	11221896	11221572	ID=Msa1313330-mRNA-1:three_prime_utr;Parent=Msa1313330-mRNA-1

Gene Sequence

>Msa1313330

ATGGACACTACTTGGGTTCTATTTGTGACACCAATTTTCTTGTGCACTTTGATCTTCTACTACAGAAACAGTCTTAGTCTTAAGCTCAAACCAAAACAAAGAAATCAGCTTCCACTAGGCACTCTTGGATGGCCTTTTATTGGCGAAACCATAGATTTTGTTTCTTGTGCTTACACCGACCGCCCTGAGAGCTTCATGACCAAGCGATGCGCCATGTATGGTAAAGTGTTCAAGTCACACATATTTGGAAGCCCTACCATAGTTTCAACAGATGCAGATGTGAACAAGTTTATACTACAAAGTGATGCAAAGGTTTTTGTTCCTTCTTATCCCAAATCATTGATGCAGTTAATGGGAGAGTCTTCTATTTTGCTCATCAATGGAACCTTGCAAAGAAGAATACATGGACTTATTGGTGCTTTCTTCAAGTCTCAACAGTTAAAGGTTCAGATTACCTCAGATATGCAGAAGTATGTACAAGAATCAATGGCAAATTGGAAAGAGGACCAACCTATATACATACAAGATGAAACAAAAAAGATTGCCTTTCATGTATTGGTTAAGGCATTGATTAGTTTGGAACCAGGTGAAGAAATGGAACTTCTAAAAAAACATTTTCAGGAATTTATCTCTGGCCTAATGTCTTTGCCAATAAACCTACCAGGAACCAAACTCTATCAATCTTTACAGGCAAGTCAAGCAAAAAAGAAAATGGTGAAATTAGTGAGAAAAACTGTCCAAGCTAAAAGAAATAAAGGCATCTTTGAAGTTCCAAGAGATGTAGTGGATGTGCTTCTTAATGATGCAAGTGAGAAATTGACAGATGACTTAATAGCAGATAATATTATTGATATGATGATTCCTGGAGAGGACTCAGTTCCAGTTCTTATGACTCTAGCTACAAAATACCTATCAGAATGTCCTCTTGCTTTACAACAATTGACGGTGGAGAATATAAAGCTCAAGAAACTCAAAGATCAGCTTGGGGGGCCTTTGTGTTGGAATGATTACTTATCATTGCCATTTACTCAAAAAGTAATCACTGAAACTTTGAGAATGGGAAATATTATAAATGGTGTTATGAGAAAGGCCTTAAAAGATGTTGAAATAAAAGGGTATTTAATACCACAAGGATGGTGTGTGTTTGCAAATTTTAGATCAGTTCATCTAGATGAGAAAAACTATGAGTGCCCATATCAATTCAATCCATGGAGGTGGCAATTAGCCACGTTTGGACCGTCTGATCAAAATAAGCAAATCTCGATTTCAAGCTCGGATAAGGACATGAACAGTTACAATTTCACTCCTTTTGGAGGAGGACAAAGGCTGTGTCCTGGTCTTGACTTGGCAAGGTTGGAAGCTTCCATCTTTCTGCATCACCTTGTTACTCAATTCAGATGGTATGCAGAAGAAGATACAATAGTGAACTTCCCCACAGTAAGAATGAAAAGAAGGATGCCTATCTTAGTAAGGAGAGTGGAAACTTAG

Protein sequence

>Msa1313330

MDTTWVLFVTPIFLCTLIFYYRNSLSLKLKPKQRNQLPLGTLGWPFIGETIDFVSCAYTDRPESFMTKRCAMYGKVFKSHIFGSPTIVSTDADVNKFILQSDAKVFVPSYPKSLMQLMGESSILLINGTLQRRIHGLIGAFFKSQQLKVQITSDMQKYVQESMANWKEDQPIYIQDETKKIAFHVLVKALISLEPGEEMELLKKHFQEFISGLMSLPINLPGTKLYQSLQASQAKKKMVKLVRKTVQAKRNKGIFEVPRDVVDVLLNDASEKLTDDLIADNIIDMMIPGEDSVPVLMTLATKYLSECPLALQQLTVENIKLKKLKDQLGGPLCWNDYLSLPFTQKVITETLRMGNIINGVMRKALKDVEIKGYLIPQGWCVFANFRSVHLDEKNYECPYQFNPWRWQLATFGPSDQNKQISISSSDKDMNSYNFTPFGGGQRLCPGLDLARLEASIFLHHLVTQFRWYAEEDTIVNFPTVRMKRRMPILVRRVET*