AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1314990

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1314990 MtrunA17_Chr8g0344441 100.000 128 0 0 1 128 1 128 3.20e-91 259
Msa1314990 MtrunA17_Chr2g0301051 82.812 128 22 0 1 128 1 128 2.35e-76 222
Msa1314990 MtrunA17_Chr4g0044211 38.356 73 44 1 14 85 7 79 1.97e-12 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1314990 AT3G14080.2 86.719 128 17 0 1 128 1 128 1.72e-80 233
Msa1314990 AT3G14080.1 86.719 128 17 0 1 128 1 128 1.72e-80 233
Msa1314990 AT1G19120.1 81.250 128 24 0 1 128 1 128 2.07e-74 217
Msa1314990 AT3G14080.4 83.621 116 17 1 15 128 3 118 1.43e-65 194
Msa1314990 AT3G14080.3 90.123 81 8 0 1 81 1 81 5.61e-50 154
Msa1314990 AT1G65700.2 38.667 75 45 1 12 85 4 78 5.58e-12 58.2
Msa1314990 AT1G65700.1 38.667 75 45 1 12 85 4 78 5.58e-12 58.2

Find 62 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGTTAATTTCTGGAAATTT+AGG 0.147955 8_4:-14137110 Msa1314990:five_prime_UTR
GAAGCGAAAGAGTGAAAATT+TGG 0.161483 8_4:+14137212 None:intergenic
TAGATCGAAATCACAATAAA+AGG 0.191374 8_4:+14137153 None:intergenic
AAGTGGGTGATTTGAGAAAA+AGG 0.224442 8_4:+14137179 None:intergenic
TCTTCTTTATATATAGGAAT+TGG 0.262226 8_4:-14137078 Msa1314990:five_prime_UTR
AGGGATGCTTCAGAACTAAA+AGG 0.302591 8_4:-14134301 Msa1314990:CDS
GGGGAAAATGTTGTTCTAAT+TGG 0.311566 8_4:-14136025 Msa1314990:CDS
AAGCGAAAGAGTGAAAATTT+GGG 0.332275 8_4:+14137213 None:intergenic
CCTAAATTTCCAGAAATTAA+CGG 0.358389 8_4:+14137110 None:intergenic
CTTCTTTATATATAGGAATT+GGG 0.377863 8_4:-14137077 Msa1314990:five_prime_UTR
TTGTTCTTCTGTGATATTGT+TGG 0.380565 8_4:-14134173 Msa1314990:three_prime_UTR
GGAATTCAATCTCAGTGAAT+AGG 0.381686 8_4:-14137056 Msa1314990:five_prime_UTR
AGAAAGCAACGGAATAATAA+TGG 0.398474 8_4:+14134099 None:intergenic
ATCACAATAAAAGGGTTAAG+TGG 0.445738 8_4:+14137162 None:intergenic
GTAGAAAGGTAGAGATCCTC+AGG 0.460456 8_4:+14136970 None:intergenic
TATCTGGTTTGGAAATGAAA+AGG 0.463066 8_4:-14134003 Msa1314990:three_prime_UTR
GATATTCAAAATGTCAGTTA+TGG 0.463126 8_4:+14134022 None:intergenic
TAACGGAATGAAAAGAACTA+AGG 0.464650 8_4:+14137127 None:intergenic
AGATCGAAATCACAATAAAA+GGG 0.480054 8_4:+14137154 None:intergenic
AAGAATGGAGTTCCTTGACT+TGG 0.481713 8_4:-14134266 Msa1314990:CDS
GCAGCTAACGCTGTTCTTGA+GGG 0.491345 8_4:-14136115 Msa1314990:intron
GTGTTCCTACAGAAGAAATC+AGG 0.501365 8_4:-14135874 Msa1314990:CDS
TTCTTGAGGGTGCCTGTGAG+AGG 0.503890 8_4:-14136102 Msa1314990:CDS
GTAGGAACACGCGTCATATG+TGG 0.510896 8_4:+14135887 None:intergenic
TGCAGCTAACGCTGTTCTTG+AGG 0.511473 8_4:-14136116 Msa1314990:intron
TATGTCTTGGGCTGCTCCTG+AGG 0.516307 8_4:-14136986 Msa1314990:CDS
GATATTTCAGGCACAGAAAG+TGG 0.522168 8_4:-14134326 Msa1314990:intron
AACTAGCAAGTGAAGTAGAA+AGG 0.522695 8_4:+14136956 None:intergenic
CTATATTGTGACATCCCTCT+AGG 0.524354 8_4:-14136064 Msa1314990:CDS
ACGAGATGGTAGAAAGCTCA+TGG 0.524959 8_4:-14136239 Msa1314990:CDS
CAATCTCAGTGAATAGGTTG+TGG 0.530649 8_4:-14137050 Msa1314990:five_prime_UTR
AAGAACAAAATAAAATAACT+TGG 0.534155 8_4:+14134188 None:intergenic
GGGATGCTTCAGAACTAAAA+GGG 0.534576 8_4:-14134300 Msa1314990:CDS
TAGGAACACGCGTCATATGT+GGG 0.543008 8_4:+14135888 None:intergenic
TCAGGCACAGAAAGTGGAAA+GGG 0.543526 8_4:-14134320 Msa1314990:intron
AAGAGCCGCTGTAATATTTG+CGG 0.553134 8_4:+14134213 None:intergenic
TCACAATAAAAGGGTTAAGT+GGG 0.553685 8_4:+14137163 None:intergenic
GCCTGTGAGAGGGTAATTGT+TGG 0.562739 8_4:-14136091 Msa1314990:CDS
AGCGAAAGAGTGAAAATTTG+GGG 0.565663 8_4:+14137214 None:intergenic
CTAGGTCTTTATGTAATCCG+TGG 0.570170 8_4:-14136046 Msa1314990:CDS
TGTTGTTCTAATTGGTGAGA+TGG 0.571580 8_4:-14136017 Msa1314990:intron
CTTCTTATATTGCTACGAGA+TGG 0.573728 8_4:-14136253 Msa1314990:CDS
TATATCACAAAATTCACCAA+TGG 0.584239 8_4:+14137022 None:intergenic
CGAGATGGTAGAAAGCTCAT+GGG 0.590247 8_4:-14136238 Msa1314990:CDS
TAGGTCTTTATGTAATCCGT+GGG 0.590765 8_4:-14136045 Msa1314990:CDS
TTCAGGCACAGAAAGTGGAA+AGG 0.592068 8_4:-14134321 Msa1314990:intron
TCTTGAGGGTGCCTGTGAGA+GGG 0.598880 8_4:-14136101 Msa1314990:CDS
TTGACAAGAAAAGAAAGCAA+CGG 0.605836 8_4:+14134088 None:intergenic
ACCCTCCTGATTTCTTCTGT+AGG 0.607048 8_4:+14135869 None:intergenic
ACCAACAATTACCCTCTCAC+AGG 0.608128 8_4:+14136090 None:intergenic
ATAGGTTGTGGCAATGCCAT+TGG 0.610982 8_4:-14137038 Msa1314990:five_prime_UTR
TATTTGCGGATGAGCGAGTG+TGG 0.613060 8_4:+14134227 None:intergenic
AGGAACACGCGTCATATGTG+GGG 0.634207 8_4:+14135889 None:intergenic
TTCCTACAGAAGAAATCAGG+AGG 0.637433 8_4:-14135871 Msa1314990:CDS
TTAATCACTAGTCCAAGTCA+AGG 0.651795 8_4:+14134254 None:intergenic
CTCATCCGCAAATATTACAG+CGG 0.668106 8_4:-14134218 Msa1314990:three_prime_UTR
GGATTACATAAAGACCTAGA+GGG 0.677541 8_4:+14136050 None:intergenic
GGAACACGCGTCATATGTGG+GGG 0.684221 8_4:+14135890 None:intergenic
CGGATTACATAAAGACCTAG+AGG 0.700189 8_4:+14136049 None:intergenic
TCCTACAGAAGAAATCAGGA+GGG 0.714873 8_4:-14135870 Msa1314990:intron
GAGATGGTAGAAAGCTCATG+GGG 0.721011 8_4:-14136237 Msa1314990:CDS
AGGTCTTTATGTAATCCGTG+GGG 0.758020 8_4:-14136044 Msa1314990:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTCTTCTTTATATAT+AGG - chr8_4:14134141-14134160 Msa1314990:three_prime_UTR 10.0%
!! AAAAATCATAATTATGTCTT+GGG - chr8_4:14134227-14134246 Msa1314990:three_prime_UTR 15.0%
!! ATTAGACAAAAATAAAAACA+TGG + chr8_4:14134334-14134353 None:intergenic 15.0%
!!! ATCATAAAGAAGTTTAATTT+AGG + chr8_4:14135389-14135408 None:intergenic 15.0%
!! TATTAATATACGACAATAAT+AGG + chr8_4:14135497-14135516 None:intergenic 15.0%
!! AAATAAAGTGGATAAATTAA+TGG - chr8_4:14135978-14135997 Msa1314990:intron 15.0%
!!! TAAGATTTTATTTTTTAAGC+AGG + chr8_4:14136639-14136658 None:intergenic 15.0%
!!! TTGATATTTTTGTTATGATT+TGG - chr8_4:14136781-14136800 Msa1314990:intron 15.0%
!! AAGAACAAAATAAAATAACT+TGG + chr8_4:14137040-14137059 None:intergenic 15.0%
!! TCTTCTTTATATATAGGAAT+TGG - chr8_4:14134147-14134166 Msa1314990:three_prime_UTR 20.0%
!! CTTCTTTATATATAGGAATT+GGG - chr8_4:14134148-14134167 Msa1314990:three_prime_UTR 20.0%
!! GAAAAATCATAATTATGTCT+TGG - chr8_4:14134226-14134245 Msa1314990:three_prime_UTR 20.0%
!! AAGAAACATATAGATATGTT+TGG - chr8_4:14134583-14134602 Msa1314990:intron 20.0%
!! TTATTAATGGTAATTAAGCT+TGG - chr8_4:14134872-14134891 Msa1314990:intron 20.0%
!! TATTAATGGTAATTAAGCTT+GGG - chr8_4:14134873-14134892 Msa1314990:intron 20.0%
!!! GAAATAGTTTTAAATGCAAA+GGG + chr8_4:14135252-14135271 None:intergenic 20.0%
!!! AGAAATAGTTTTAAATGCAA+AGG + chr8_4:14135253-14135272 None:intergenic 20.0%
!!! AAAAGTACAATAATTGTGTT+TGG + chr8_4:14135722-14135741 None:intergenic 20.0%
!!! TTCAAGGATATATCAAATTT+TGG + chr8_4:14136595-14136614 None:intergenic 20.0%
!!! TGAAATTTAGGATTAATTCA+AGG + chr8_4:14136611-14136630 None:intergenic 20.0%
!! TTGAATTAATCCTAAATTTC+AGG - chr8_4:14136610-14136629 Msa1314990:intron 20.0%
!! ATCATAACAAAAATATCAAC+TGG + chr8_4:14136780-14136799 None:intergenic 20.0%
!! GTTAAATATTCTATATTGAG+TGG - chr8_4:14136870-14136889 Msa1314990:intron 20.0%
!!! AAATTTGGGGATTTTGAAAT+GGG + chr8_4:14134001-14134020 None:intergenic 25.0%
!!! AAAATTTGGGGATTTTGAAA+TGG + chr8_4:14134002-14134021 None:intergenic 25.0%
! AGATCGAAATCACAATAAAA+GGG + chr8_4:14134074-14134093 None:intergenic 25.0%
! TAGATCGAAATCACAATAAA+AGG + chr8_4:14134075-14134094 None:intergenic 25.0%
! CCTAAATTTCCAGAAATTAA+CGG + chr8_4:14134118-14134137 None:intergenic 25.0%
! TATATCACAAAATTCACCAA+TGG + chr8_4:14134206-14134225 None:intergenic 25.0%
! ATAATTAAAAAAGACCCGTT+TGG - chr8_4:14134390-14134409 Msa1314990:intron 25.0%
! ATAAATAAGTCAATCCAAAC+AGG + chr8_4:14134460-14134479 None:intergenic 25.0%
! TATCTATATGTTTCTTGTCA+AGG + chr8_4:14134579-14134598 None:intergenic 25.0%
! TGATGAAAACCAGTTATTAA+TGG - chr8_4:14134859-14134878 Msa1314990:intron 25.0%
! GCTTAATTACCATTAATAAC+TGG + chr8_4:14134871-14134890 None:intergenic 25.0%
! TAGTCAGTTAGAAATTAGAT+TGG - chr8_4:14135611-14135630 Msa1314990:intron 25.0%
! GTGATATCTACTAATTGTTA+TGG - chr8_4:14135746-14135765 Msa1314990:intron 25.0%
!! TGCATACTTCATAATCATTT+TGG - chr8_4:14136001-14136020 Msa1314990:intron 25.0%
! TAACTAATTAGCTACAACTA+AGG + chr8_4:14136276-14136295 None:intergenic 25.0%
! TTGGTCAATTTATACATTGA+AGG - chr8_4:14136462-14136481 Msa1314990:intron 25.0%
! TTATGTATTACCACTGTAAA+TGG + chr8_4:14136525-14136544 None:intergenic 25.0%
! GATATTCAAAATGTCAGTTA+TGG + chr8_4:14137206-14137225 None:intergenic 25.0%
!! TAACTGACATTTTGAATATC+TGG - chr8_4:14137206-14137225 Msa1314990:five_prime_UTR 25.0%
AAGCGAAAGAGTGAAAATTT+GGG + chr8_4:14134015-14134034 None:intergenic 30.0%
TCACAATAAAAGGGTTAAGT+GGG + chr8_4:14134065-14134084 None:intergenic 30.0%
ATCACAATAAAAGGGTTAAG+TGG + chr8_4:14134066-14134085 None:intergenic 30.0%
TAACGGAATGAAAAGAACTA+AGG + chr8_4:14134101-14134120 None:intergenic 30.0%
! CTTTTCATTCCGTTAATTTC+TGG - chr8_4:14134106-14134125 Msa1314990:three_prime_UTR 30.0%
! CCGTTAATTTCTGGAAATTT+AGG - chr8_4:14134115-14134134 Msa1314990:three_prime_UTR 30.0%
CAGCTTATAAGCTATAATCA+AGG + chr8_4:14134368-14134387 None:intergenic 30.0%
CTTGATTATAGCTTATAAGC+TGG - chr8_4:14134366-14134385 Msa1314990:intron 30.0%
AATAAGTTCGTGTTACCAAA+CGG + chr8_4:14134408-14134427 None:intergenic 30.0%
TTTCTTGTCAAGGTAGTTTA+CGG + chr8_4:14134569-14134588 None:intergenic 30.0%
TACCTATCAACTTAAGCTTT+GGG - chr8_4:14134662-14134681 Msa1314990:intron 30.0%
TCAGTAGTAGAACATAAGAT+TGG + chr8_4:14134935-14134954 None:intergenic 30.0%
ACATCCTGAAAATTGAAAGA+CGG + chr8_4:14135299-14135318 None:intergenic 30.0%
CATTGTTTGTAAAGAGAAGT+AGG + chr8_4:14135525-14135544 None:intergenic 30.0%
CTACTTCTCTTTACAAACAA+TGG - chr8_4:14135523-14135542 Msa1314990:intron 30.0%
! GAAATTAGATTGGTCATGTT+TGG - chr8_4:14135621-14135640 Msa1314990:intron 30.0%
ATTGTTATGGTAAGTTACCT+TGG - chr8_4:14135759-14135778 Msa1314990:intron 30.0%
TACGATTATTACGATGATGA+TGG - chr8_4:14135804-14135823 Msa1314990:intron 30.0%
ACGATTATTACGATGATGAT+GGG - chr8_4:14135805-14135824 Msa1314990:intron 30.0%
! GATATAGTGTGGATTACTTT+AGG + chr8_4:14135886-14135905 None:intergenic 30.0%
TTAAGCTAGTACGACTTATT+AGG - chr8_4:14136079-14136098 Msa1314990:CDS 30.0%
TAAGCTAGTACGACTTATTA+GGG - chr8_4:14136080-14136099 Msa1314990:CDS 30.0%
! AGATGTTTTCTTTGTGTAGT+TGG - chr8_4:14136117-14136136 Msa1314990:intron 30.0%
ACTTGAGAATATAGCTTAGT+TGG - chr8_4:14136200-14136219 Msa1314990:intron 30.0%
TACTATATTGAAATGCTTCG+TGG - chr8_4:14136430-14136449 Msa1314990:intron 30.0%
TATAACTCAACCATTTACAG+TGG - chr8_4:14136512-14136531 Msa1314990:intron 30.0%
GATATGTTAAAGAGGTCATT+TGG + chr8_4:14136553-14136572 None:intergenic 30.0%
GCAATGTAGATATGTTAAAG+AGG + chr8_4:14136561-14136580 None:intergenic 30.0%
TACAATGGACAAGTAGTTTA+TGG + chr8_4:14136671-14136690 None:intergenic 30.0%
TTGTCCATTGTACATATGAT+TGG - chr8_4:14136679-14136698 Msa1314990:intron 30.0%
CTTACCAATCATATGTACAA+TGG + chr8_4:14136686-14136705 None:intergenic 30.0%
TTGTTCTTCTGTGATATTGT+TGG - chr8_4:14137052-14137071 Msa1314990:five_prime_UTR 30.0%
!!! TGTGATATTGTTGGTTTTCA+TGG - chr8_4:14137061-14137080 Msa1314990:five_prime_UTR 30.0%
!!! GTGATATTGTTGGTTTTCAT+GGG - chr8_4:14137062-14137081 Msa1314990:five_prime_UTR 30.0%
AGAAAGCAACGGAATAATAA+TGG + chr8_4:14137129-14137148 None:intergenic 30.0%
TTGACAAGAAAAGAAAGCAA+CGG + chr8_4:14137140-14137159 None:intergenic 30.0%
! TGCTTTCTTTTCTTGTCAAA+AGG - chr8_4:14137141-14137160 Msa1314990:five_prime_UTR 30.0%
! GACATTTTGAATATCTGGTT+TGG - chr8_4:14137211-14137230 Msa1314990:five_prime_UTR 30.0%
!! TATCTGGTTTGGAAATGAAA+AGG - chr8_4:14137222-14137241 Msa1314990:five_prime_UTR 30.0%
AGCGAAAGAGTGAAAATTTG+GGG + chr8_4:14134014-14134033 None:intergenic 35.0%
GAAGCGAAAGAGTGAAAATT+TGG + chr8_4:14134016-14134035 None:intergenic 35.0%
AGTGGGTGATTTGAGAAAAA+GGG + chr8_4:14134048-14134067 None:intergenic 35.0%
AAGTGGGTGATTTGAGAAAA+AGG + chr8_4:14134049-14134068 None:intergenic 35.0%
GGAATTCAATCTCAGTGAAT+AGG - chr8_4:14134169-14134188 Msa1314990:three_prime_UTR 35.0%
AACTAGCAAGTGAAGTAGAA+AGG + chr8_4:14134272-14134291 None:intergenic 35.0%
ATAAGTTCGTGTTACCAAAC+GGG + chr8_4:14134407-14134426 None:intergenic 35.0%
AAGTAGCTTATTGACCTGTT+TGG - chr8_4:14134443-14134462 Msa1314990:intron 35.0%
TTCTTGTCAAGGTAGTTTAC+GGG + chr8_4:14134568-14134587 None:intergenic 35.0%
AGCACATGAATGGTTACAAA+AGG + chr8_4:14134643-14134662 None:intergenic 35.0%
CTACCTATCAACTTAAGCTT+TGG - chr8_4:14134661-14134680 Msa1314990:intron 35.0%
ACCTATCAACTTAAGCTTTG+GGG - chr8_4:14134663-14134682 Msa1314990:intron 35.0%
CTTCTTATATTGCTACGAGA+TGG - chr8_4:14134972-14134991 Msa1314990:intron 35.0%
! CTTCGCTCTTTTGATCAATT+TGG - chr8_4:14135014-14135033 Msa1314990:intron 35.0%
GTAAAGATTTGACATGCGTT+AGG - chr8_4:14135056-14135075 Msa1314990:intron 35.0%
GTTAGCTGCATGTTATTGAT+AGG + chr8_4:14135099-14135118 None:intergenic 35.0%
GGATTACATAAAGACCTAGA+GGG + chr8_4:14135178-14135197 None:intergenic 35.0%
TAGGTCTTTATGTAATCCGT+GGG - chr8_4:14135180-14135199 Msa1314990:intron 35.0%
! TTAGAACAACATTTTCCCCA+CGG + chr8_4:14135199-14135218 None:intergenic 35.0%
GGGGAAAATGTTGTTCTAAT+TGG - chr8_4:14135200-14135219 Msa1314990:intron 35.0%
TGTTGTTCTAATTGGTGAGA+TGG - chr8_4:14135208-14135227 Msa1314990:intron 35.0%
! ACAACCGTCTTTCAATTTTC+AGG - chr8_4:14135292-14135311 Msa1314990:intron 35.0%
!! GTCTTTCAATTTTCAGGATG+TGG - chr8_4:14135298-14135317 Msa1314990:intron 35.0%
CATTGTATTGCCTTAAGTGA+GGG + chr8_4:14135445-14135464 None:intergenic 35.0%
ACATTGTATTGCCTTAAGTG+AGG + chr8_4:14135446-14135465 None:intergenic 35.0%
TCTACATGAAACAGATTCGT+AGG - chr8_4:14135579-14135598 Msa1314990:intron 35.0%
CTACATGAAACAGATTCGTA+GGG - chr8_4:14135580-14135599 Msa1314990:intron 35.0%
TGTAACTCTAACTCTGTAAC+TGG + chr8_4:14135647-14135666 None:intergenic 35.0%
CAGAGTTAGAGTTACAGAAT+TGG - chr8_4:14135651-14135670 Msa1314990:intron 35.0%
! GTTACAGAATTGGCTTAGAT+AGG - chr8_4:14135661-14135680 Msa1314990:intron 35.0%
TAGGAGGTTATCTCATAACT+CGG - chr8_4:14135680-14135699 Msa1314990:intron 35.0%
!! TTACCTTGGCTTGTGAAATA+AGG - chr8_4:14135773-14135792 Msa1314990:intron 35.0%
!! TACCTTGGCTTGTGAAATAA+GGG - chr8_4:14135774-14135793 Msa1314990:intron 35.0%
CTAGCTTAATCGCTAGAATT+TGG + chr8_4:14136068-14136087 None:intergenic 35.0%
AATTAGCTACAACTAAGGAC+AGG + chr8_4:14136271-14136290 None:intergenic 35.0%
! TGCTTCGTGGTTATGATTTT+TGG - chr8_4:14136443-14136462 Msa1314990:intron 35.0%
AAGCAGGATTCCTGAAATTT+AGG + chr8_4:14136623-14136642 None:intergenic 35.0%
! ATATGATTGGTAAGCTCTAG+TGG - chr8_4:14136692-14136711 Msa1314990:intron 35.0%
TGTATGACTGCATGTCAAAA+CGG + chr8_4:14136824-14136843 None:intergenic 35.0%
GAGTGGAAATGTGATATTTC+AGG - chr8_4:14136887-14136906 Msa1314990:intron 35.0%
AGGGACTATGAGAAAAAGAA+TGG - chr8_4:14136944-14136963 Msa1314990:intron 35.0%
TTAATCACTAGTCCAAGTCA+AGG + chr8_4:14136974-14136993 None:intergenic 35.0%
!!! ATATTGTTGGTTTTCATGGG+AGG - chr8_4:14137065-14137084 Msa1314990:five_prime_UTR 35.0%
!!! TATTGTTGGTTTTCATGGGA+GGG - chr8_4:14137066-14137085 Msa1314990:five_prime_UTR 35.0%
CAATCTCAGTGAATAGGTTG+TGG - chr8_4:14134175-14134194 Msa1314990:three_prime_UTR 40.0%
! GTTGATAGGTAGCACATGAA+TGG + chr8_4:14134653-14134672 None:intergenic 40.0%
!! ACCCCAAAGCTTAAGTTGAT+AGG + chr8_4:14134667-14134686 None:intergenic 40.0%
ATCTTGCACACAAGTCTTTC+TGG - chr8_4:14134802-14134821 Msa1314990:intron 40.0%
CTATATTGTGACATCCCTCT+AGG - chr8_4:14135161-14135180 Msa1314990:intron 40.0%
CGGATTACATAAAGACCTAG+AGG + chr8_4:14135179-14135198 None:intergenic 40.0%
CTAGGTCTTTATGTAATCCG+TGG - chr8_4:14135179-14135198 Msa1314990:intron 40.0%
AGGTCTTTATGTAATCCGTG+GGG - chr8_4:14135181-14135200 Msa1314990:intron 40.0%
!! TCAATTTTCAGGATGTGGAG+AGG - chr8_4:14135303-14135322 Msa1314990:intron 40.0%
!! CAATTTTCAGGATGTGGAGA+GGG - chr8_4:14135304-14135323 Msa1314990:intron 40.0%
GTGTTCCTACAGAAGAAATC+AGG - chr8_4:14135351-14135370 Msa1314990:intron 40.0%
TTCCTACAGAAGAAATCAGG+AGG - chr8_4:14135354-14135373 Msa1314990:intron 40.0%
TCCTACAGAAGAAATCAGGA+GGG - chr8_4:14135355-14135374 Msa1314990:intron 40.0%
ATAGGCACTTCTGCAATGAA+TGG + chr8_4:14135479-14135498 None:intergenic 40.0%
! ACAGAATTGGCTTAGATAGG+AGG - chr8_4:14135664-14135683 Msa1314990:intron 40.0%
! TACCCTTATTTCACAAGCCA+AGG + chr8_4:14135779-14135798 None:intergenic 40.0%
!! ATTACGATGATGATGGGTGT+TGG - chr8_4:14135811-14135830 Msa1314990:intron 40.0%
AGGTAAAGTCGGATATAGTG+TGG + chr8_4:14135897-14135916 None:intergenic 40.0%
CACTATATCCGACTTTACCT+TGG - chr8_4:14135897-14135916 Msa1314990:CDS 40.0%
GCAACGAGCTAGAAATAAAG+TGG - chr8_4:14135966-14135985 Msa1314990:intron 40.0%
AGTACGACTTATTAGGGATG+CGG - chr8_4:14136086-14136105 Msa1314990:CDS 40.0%
TTTCACTCTCTGATTCACCA+GGG - chr8_4:14136368-14136387 Msa1314990:intron 40.0%
GATATTTCAGGCACAGAAAG+TGG - chr8_4:14136899-14136918 Msa1314990:intron 40.0%
AGGGATGCTTCAGAACTAAA+AGG - chr8_4:14136924-14136943 Msa1314990:intron 40.0%
GGGATGCTTCAGAACTAAAA+GGG - chr8_4:14136925-14136944 Msa1314990:intron 40.0%
AAGAATGGAGTTCCTTGACT+TGG - chr8_4:14136959-14136978 Msa1314990:CDS 40.0%
CTCATCCGCAAATATTACAG+CGG - chr8_4:14137007-14137026 Msa1314990:exon 40.0%
AAGAGCCGCTGTAATATTTG+CGG + chr8_4:14137015-14137034 None:intergenic 40.0%
ATAGGTTGTGGCAATGCCAT+TGG - chr8_4:14134187-14134206 Msa1314990:three_prime_UTR 45.0%
GTAGAAAGGTAGAGATCCTC+AGG + chr8_4:14134258-14134277 None:intergenic 45.0%
!! GGTTACAAAAGGCATCTCTC+AGG + chr8_4:14134632-14134651 None:intergenic 45.0%
ACGAGATGGTAGAAAGCTCA+TGG - chr8_4:14134986-14135005 Msa1314990:intron 45.0%
CGAGATGGTAGAAAGCTCAT+GGG - chr8_4:14134987-14135006 Msa1314990:intron 45.0%
GAGATGGTAGAAAGCTCATG+GGG - chr8_4:14134988-14135007 Msa1314990:intron 45.0%
ACCAACAATTACCCTCTCAC+AGG + chr8_4:14135138-14135157 None:intergenic 45.0%
TAGGAACACGCGTCATATGT+GGG + chr8_4:14135340-14135359 None:intergenic 45.0%
ACCCTCCTGATTTCTTCTGT+AGG + chr8_4:14135359-14135378 None:intergenic 45.0%
CTTAAGTGAGGGCAAGAACA+AGG + chr8_4:14135434-14135453 None:intergenic 45.0%
CTTGTTCTTGCCCTCACTTA+AGG - chr8_4:14135432-14135451 Msa1314990:intron 45.0%
GGCAATACAATGTTGTGCTG+TGG - chr8_4:14135453-14135472 Msa1314990:intron 45.0%
CAGTGGATCCAAGGTAAAGT+CGG + chr8_4:14135908-14135927 None:intergenic 45.0%
AAACTCTTGCAGTGGATCCA+AGG + chr8_4:14135917-14135936 None:intergenic 45.0%
GTGCTGTAAAACTCTTGCAG+TGG + chr8_4:14135925-14135944 None:intergenic 45.0%
CCATACTAGTACAGGCAACT+TGG + chr8_4:14135947-14135966 None:intergenic 45.0%
CCAAGTTGCCTGTACTAGTA+TGG - chr8_4:14135944-14135963 Msa1314990:intron 45.0%
CTTTCACTCTCTGATTCACC+AGG - chr8_4:14136367-14136386 Msa1314990:intron 45.0%
ACTGCTAAAGCAAGTATCCC+TGG + chr8_4:14136388-14136407 None:intergenic 45.0%
TTCAGGCACAGAAAGTGGAA+AGG - chr8_4:14136904-14136923 Msa1314990:intron 45.0%
TCAGGCACAGAAAGTGGAAA+GGG - chr8_4:14136905-14136924 Msa1314990:intron 45.0%
TGCAGCTAACGCTGTTCTTG+AGG - chr8_4:14135109-14135128 Msa1314990:intron 50.0%
GCAGCTAACGCTGTTCTTGA+GGG - chr8_4:14135110-14135129 Msa1314990:intron 50.0%
GCCTGTGAGAGGGTAATTGT+TGG - chr8_4:14135134-14135153 Msa1314990:intron 50.0%
!! TTTTCAGGATGTGGAGAGGG+AGG - chr8_4:14135307-14135326 Msa1314990:intron 50.0%
AGGAACACGCGTCATATGTG+GGG + chr8_4:14135339-14135358 None:intergenic 50.0%
GTAGGAACACGCGTCATATG+TGG + chr8_4:14135341-14135360 None:intergenic 50.0%
!! ACGATGATGATGGGTGTTGG+TGG - chr8_4:14135814-14135833 Msa1314990:intron 50.0%
!! TGGTGGCTTGGTGCAATAGT+TGG - chr8_4:14135831-14135850 Msa1314990:intron 50.0%
GCTCGTTGCCATACTAGTAC+AGG + chr8_4:14135955-14135974 None:intergenic 50.0%
GCATGTCAAAACGGACGACA+TGG + chr8_4:14136815-14136834 None:intergenic 50.0%
TATTTGCGGATGAGCGAGTG+TGG + chr8_4:14137001-14137020 None:intergenic 50.0%
TATGTCTTGGGCTGCTCCTG+AGG - chr8_4:14134239-14134258 Msa1314990:three_prime_UTR 55.0%
!! TTCTTGAGGGTGCCTGTGAG+AGG - chr8_4:14135123-14135142 Msa1314990:intron 55.0%
!! TCTTGAGGGTGCCTGTGAGA+GGG - chr8_4:14135124-14135143 Msa1314990:intron 55.0%
GGAACACGCGTCATATGTGG+GGG + chr8_4:14135338-14135357 None:intergenic 55.0%
!! GATGATGGGTGTTGGTGGCT+TGG - chr8_4:14135819-14135838 Msa1314990:intron 55.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 14133989 14137258 14133989 ID=Msa1314990;Name=Msa1314990
chr8_4 mRNA 14133989 14137258 14133989 ID=Msa1314990-mRNA-1;Parent=Msa1314990;Name=Msa1314990-mRNA-1;_AED=0.14;_eAED=0.14;_QI=251|1|1|1|1|1|5|272|128
chr8_4 exon 14133989 14134338 14133989 ID=Msa1314990-mRNA-1:exon:5340;Parent=Msa1314990-mRNA-1
chr8_4 exon 14135871 14135933 14135871 ID=Msa1314990-mRNA-1:exon:5339;Parent=Msa1314990-mRNA-1
chr8_4 exon 14136018 14136133 14136018 ID=Msa1314990-mRNA-1:exon:5338;Parent=Msa1314990-mRNA-1
chr8_4 exon 14136212 14136280 14136212 ID=Msa1314990-mRNA-1:exon:5337;Parent=Msa1314990-mRNA-1
chr8_4 exon 14136947 14137258 14136947 ID=Msa1314990-mRNA-1:exon:5336;Parent=Msa1314990-mRNA-1
chr8_4 five_prime_UTR 14137008 14137258 14137008 ID=Msa1314990-mRNA-1:five_prime_utr;Parent=Msa1314990-mRNA-1
chr8_4 CDS 14136947 14137007 14136947 ID=Msa1314990-mRNA-1:cds;Parent=Msa1314990-mRNA-1
chr8_4 CDS 14136212 14136280 14136212 ID=Msa1314990-mRNA-1:cds;Parent=Msa1314990-mRNA-1
chr8_4 CDS 14136018 14136133 14136018 ID=Msa1314990-mRNA-1:cds;Parent=Msa1314990-mRNA-1
chr8_4 CDS 14135871 14135933 14135871 ID=Msa1314990-mRNA-1:cds;Parent=Msa1314990-mRNA-1
chr8_4 CDS 14134261 14134338 14134261 ID=Msa1314990-mRNA-1:cds;Parent=Msa1314990-mRNA-1
chr8_4 three_prime_UTR 14133989 14134260 14133989 ID=Msa1314990-mRNA-1:three_prime_utr;Parent=Msa1314990-mRNA-1
Gene Sequence

>Msa1314990

ATGTCTTGGGCTGCTCCTGAGGATCTCTACCTTTCTACTTCACTTGCTAGTTATCTTGACAAGAAACTTCTTATATTGCTACGAGATGGTAGAAAGCTCATGGGGACACTTCGCTCTTTTGATCAATTTGCTAACGCTGTTCTTGAGGGTGCCTGTGAGAGGGTAATTGTTGGTGATCTATATTGTGACATCCCTCTAGGTCTTTATGTAATCCGTGGGGAAAATGTTGTTCTAATTGGTGAGATGGATGTGGAGAGGGAGGAACTTCCCCCACATATGACGCGTGTTCCTACAGAAGAAATCAGGAGGGCACAGAAAGTGGAAAGGGATGCTTCAGAACTAAAAGGGACTATGAGAAAAAGAATGGAGTTCCTTGACTTGGACTAG
Protein sequence

>Msa1314990

MSWAAPEDLYLSTSLASYLDKKLLILLRDGRKLMGTLRSFDQFANAVLEGACERVIVGDLYCDIPLGLYVIRGENVVLIGEMDVEREELPPHMTRVPTEEIRRAQKVERDASELKGTMRKRMEFLDLD*