AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1318450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1318450 MtrunA17_Chr8g0349051 100.000 82 0 0 1 82 1 82 4.30e-50 152
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1318450 AT2G45860.1 65.432 81 25 1 1 81 1 78 6.39e-11 53.5

Find 31 sgRNAs with CRISPR-Local

Find 283 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCCAAATTCATACAAATT+AGG 0.156463 8_4:+20462887 None:intergenic
CTTAAAATGGTATCCCTTTC+CGG 0.185449 8_4:-20464784 None:intergenic
GGGTGGTGGAAGTGGATTTA+AGG 0.234381 8_4:+20464398 Msa1318450:CDS
TCCCAAATTCATACAAATTA+GGG 0.240413 8_4:+20462888 None:intergenic
CTAATCAGACGGCCAGAAAA+AGG 0.300444 8_4:-20464817 None:intergenic
AGGACTCGTTATCCTTAAAA+TGG 0.309268 8_4:-20464797 None:intergenic
GGAAAATTTGTTTAGGTTGA+TGG 0.363350 8_4:+20464357 Msa1318450:intron
GCAATGGAGCTCGACAAATA+AGG 0.389856 8_4:+20464480 Msa1318450:CDS
ATGAAATTCACCACCGGAAA+GGG 0.415538 8_4:+20464771 Msa1318450:three_prime_UTR
GAAGAAAGAAAAGAAGCTTC+AGG 0.431096 8_4:+20463134 Msa1318450:CDS
AGCTAAAGCTGCGCAGGCAA+TGG 0.436809 8_4:+20464464 Msa1318450:CDS
AATGTAACACACGTTTGGTT+TGG 0.441962 8_4:+20464861 Msa1318450:three_prime_UTR
TGTTAAGTTATTTGACTATG+AGG 0.443416 8_4:+20464603 Msa1318450:three_prime_UTR
TGGTTGATTTGAGACAAAAT+TGG 0.443540 8_4:-20464678 None:intergenic
ATCTATTAATTTCACTGCAT+CGG 0.452439 8_4:-20464539 None:intergenic
AGTCCAATGTAACACACGTT+TGG 0.460386 8_4:+20464856 Msa1318450:three_prime_UTR
TTTCGACAGGGCTGACAACC+TGG 0.508262 8_4:-20464442 None:intergenic
GTCGAAAGCTAAAGCTGCGC+AGG 0.513870 8_4:+20464458 Msa1318450:CDS
CGCAGCTTTAGCTTTCGACA+GGG 0.523634 8_4:-20464454 None:intergenic
TAATTTCACTGCATCGGCAT+CGG 0.526109 8_4:-20464533 None:intergenic
AAACCAAACGTGTGTTACAT+TGG 0.526205 8_4:-20464859 None:intergenic
TGGTGGAAGTGGATTTAAGG+TGG 0.555596 8_4:+20464401 Msa1318450:CDS
CAATGTCTAAATAAATGTGT+TGG 0.566363 8_4:+20464891 Msa1318450:three_prime_UTR
TAATATATTGTCTAATCAGA+CGG 0.571590 8_4:-20464828 None:intergenic
GGTGGAAGTGGATTTAAGGT+GGG 0.573086 8_4:+20464402 Msa1318450:CDS
ACAAATAAGGCGTCATTGTG+AGG 0.578453 8_4:+20464493 Msa1318450:exon
CACAGAATGAAATTCACCAC+CGG 0.607378 8_4:+20464765 Msa1318450:three_prime_UTR
AATGAAATTCACCACCGGAA+AGG 0.615913 8_4:+20464770 Msa1318450:three_prime_UTR
GAAAGAAATATCTGAAGACC+AGG 0.618159 8_4:+20464424 Msa1318450:CDS
TAGATGACATAGCTGTTCTG+AGG 0.624077 8_4:+20464557 Msa1318450:three_prime_UTR
AAAATGGTATCCCTTTCCGG+TGG 0.682021 8_4:-20464781 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATGAAAAATTAGAAAATT+AGG - chr8_4:20463063-20463082 None:intergenic 10.0%
!!! TTTTGTTTTTTAAATGATAT+TGG + chr8_4:20463399-20463418 Msa1318450:intron 10.0%
!! AAATGAAAAATTAGAAAATT+AGG - chr8_4:20463063-20463082 None:intergenic 10.0%
!!! TTTTGTTTTTTAAATGATAT+TGG + chr8_4:20463399-20463418 Msa1318450:intron 10.0%
!!! ATTAACTCTAAAACAAATAT+AGG - chr8_4:20463438-20463457 None:intergenic 15.0%
!! ATCTATGAAAATAAAAGTAA+TGG + chr8_4:20463692-20463711 Msa1318450:intron 15.0%
!!! AGATATAAATAAAAAGTACA+TGG - chr8_4:20464701-20464720 None:intergenic 15.0%
!!! ATTAACTCTAAAACAAATAT+AGG - chr8_4:20463438-20463457 None:intergenic 15.0%
!! ATCTATGAAAATAAAAGTAA+TGG + chr8_4:20463692-20463711 Msa1318450:intron 15.0%
!!! AGATATAAATAAAAAGTACA+TGG - chr8_4:20464701-20464720 None:intergenic 15.0%
!!! AGAAAATTAGGTTTGAAATT+GGG - chr8_4:20463051-20463070 None:intergenic 20.0%
!!! TAGAAAATTAGGTTTGAAAT+TGG - chr8_4:20463052-20463071 None:intergenic 20.0%
!!! TTTTTGTAATGATTTCTTTC+AGG + chr8_4:20463093-20463112 Msa1318450:intron 20.0%
!!! AAAAAGATAGTGTATGATTT+TGG + chr8_4:20463214-20463233 Msa1318450:intron 20.0%
!!! AAAAGATAGTGTATGATTTT+GGG + chr8_4:20463215-20463234 Msa1318450:intron 20.0%
!!! TTGTGAACTTTTTGTTTAAT+GGG + chr8_4:20463282-20463301 Msa1318450:intron 20.0%
!!! TTTTTGTTTAATGGGATAAT+GGG + chr8_4:20463290-20463309 Msa1318450:intron 20.0%
!! GTTAATTGATGAACATAAAT+TGG + chr8_4:20463370-20463389 Msa1318450:intron 20.0%
!! AATATAAAAGTTCACAACTT+TGG - chr8_4:20463474-20463493 None:intergenic 20.0%
!!! TTTTATATTACTGAGATTGT+GGG + chr8_4:20463485-20463504 Msa1318450:intron 20.0%
!! AAATACCAAAAAGTAGATTA+TGG - chr8_4:20463515-20463534 None:intergenic 20.0%
!! TATTTATAGAGTAGGTTTAA+AGG + chr8_4:20463959-20463978 Msa1318450:intron 20.0%
!! TGGATATTAGATTTAAACTT+TGG + chr8_4:20464233-20464252 Msa1318450:intron 20.0%
!! TATTAGATTTAAACTTTGGA+AGG + chr8_4:20464237-20464256 Msa1318450:intron 20.0%
!! TAATATATTGTCTAATCAGA+CGG - chr8_4:20464831-20464850 None:intergenic 20.0%
!!! AGAAAATTAGGTTTGAAATT+GGG - chr8_4:20463051-20463070 None:intergenic 20.0%
!!! TAGAAAATTAGGTTTGAAAT+TGG - chr8_4:20463052-20463071 None:intergenic 20.0%
!!! TTTTTGTAATGATTTCTTTC+AGG + chr8_4:20463093-20463112 Msa1318450:intron 20.0%
!!! AAAAAGATAGTGTATGATTT+TGG + chr8_4:20463214-20463233 Msa1318450:intron 20.0%
!!! AAAAGATAGTGTATGATTTT+GGG + chr8_4:20463215-20463234 Msa1318450:intron 20.0%
!!! TTGTGAACTTTTTGTTTAAT+GGG + chr8_4:20463282-20463301 Msa1318450:intron 20.0%
!!! TTTTTGTTTAATGGGATAAT+GGG + chr8_4:20463290-20463309 Msa1318450:intron 20.0%
!! GTTAATTGATGAACATAAAT+TGG + chr8_4:20463370-20463389 Msa1318450:intron 20.0%
!! AATATAAAAGTTCACAACTT+TGG - chr8_4:20463474-20463493 None:intergenic 20.0%
!!! TTTTATATTACTGAGATTGT+GGG + chr8_4:20463485-20463504 Msa1318450:intron 20.0%
!! AAATACCAAAAAGTAGATTA+TGG - chr8_4:20463515-20463534 None:intergenic 20.0%
!! TATTTATAGAGTAGGTTTAA+AGG + chr8_4:20463959-20463978 Msa1318450:intron 20.0%
!! TGGATATTAGATTTAAACTT+TGG + chr8_4:20464233-20464252 Msa1318450:intron 20.0%
!! TATTAGATTTAAACTTTGGA+AGG + chr8_4:20464237-20464256 Msa1318450:intron 20.0%
!! TAATATATTGTCTAATCAGA+CGG - chr8_4:20464831-20464850 None:intergenic 20.0%
!! AAAATTAGGTTTGAAATTGG+GGG - chr8_4:20463049-20463068 None:intergenic 25.0%
!! GAAAATTAGGTTTGAAATTG+GGG - chr8_4:20463050-20463069 None:intergenic 25.0%
! AAACAAAAAGTTCACAACTT+TGG - chr8_4:20463279-20463298 None:intergenic 25.0%
!!! GTTGTGAACTTTTTGTTTAA+TGG + chr8_4:20463281-20463300 Msa1318450:intron 25.0%
!!! CTTTTTGTTTAATGGGATAA+TGG + chr8_4:20463289-20463308 Msa1318450:intron 25.0%
! TTAAATGATATTGGTGAGTT+TGG + chr8_4:20463408-20463427 Msa1318450:intron 25.0%
! TAAATGATATTGGTGAGTTT+GGG + chr8_4:20463409-20463428 Msa1318450:intron 25.0%
!! CTTTTATATTACTGAGATTG+TGG + chr8_4:20463484-20463503 Msa1318450:intron 25.0%
!! CAAATTCAACACCTAATTTT+CGG - chr8_4:20463567-20463586 None:intergenic 25.0%
! ACTCTATAAATACTAGCTAA+TGG - chr8_4:20463951-20463970 None:intergenic 25.0%
! TTAGCTAGTATTTATAGAGT+AGG + chr8_4:20463951-20463970 Msa1318450:intron 25.0%
!!! GATTGAAAGTGTTTTGATAT+GGG - chr8_4:20464154-20464173 None:intergenic 25.0%
!!! AGATTGAAAGTGTTTTGATA+TGG - chr8_4:20464155-20464174 None:intergenic 25.0%
! AAAACACTTTCAATCTAAAG+TGG + chr8_4:20464159-20464178 Msa1318450:intron 25.0%
!!! TTTTGCTGGAAAATTTGTTT+AGG + chr8_4:20464350-20464369 Msa1318450:intron 25.0%
! ATCTATTAATTTCACTGCAT+CGG - chr8_4:20464542-20464561 None:intergenic 25.0%
! TGTTAAGTTATTTGACTATG+AGG + chr8_4:20464603-20464622 Msa1318450:three_prime_UTR 25.0%
!! ATTGATGAAATTTTACATGC+TGG + chr8_4:20464729-20464748 Msa1318450:three_prime_UTR 25.0%
! CAATGTCTAAATAAATGTGT+TGG + chr8_4:20464891-20464910 Msa1318450:three_prime_UTR 25.0%
!! AAAATTAGGTTTGAAATTGG+GGG - chr8_4:20463049-20463068 None:intergenic 25.0%
!! GAAAATTAGGTTTGAAATTG+GGG - chr8_4:20463050-20463069 None:intergenic 25.0%
! AAACAAAAAGTTCACAACTT+TGG - chr8_4:20463279-20463298 None:intergenic 25.0%
!!! GTTGTGAACTTTTTGTTTAA+TGG + chr8_4:20463281-20463300 Msa1318450:intron 25.0%
!!! CTTTTTGTTTAATGGGATAA+TGG + chr8_4:20463289-20463308 Msa1318450:intron 25.0%
! TTAAATGATATTGGTGAGTT+TGG + chr8_4:20463408-20463427 Msa1318450:intron 25.0%
! TAAATGATATTGGTGAGTTT+GGG + chr8_4:20463409-20463428 Msa1318450:intron 25.0%
!! CTTTTATATTACTGAGATTG+TGG + chr8_4:20463484-20463503 Msa1318450:intron 25.0%
!! CAAATTCAACACCTAATTTT+CGG - chr8_4:20463567-20463586 None:intergenic 25.0%
! ACTCTATAAATACTAGCTAA+TGG - chr8_4:20463951-20463970 None:intergenic 25.0%
! TTAGCTAGTATTTATAGAGT+AGG + chr8_4:20463951-20463970 Msa1318450:intron 25.0%
!!! GATTGAAAGTGTTTTGATAT+GGG - chr8_4:20464154-20464173 None:intergenic 25.0%
!!! AGATTGAAAGTGTTTTGATA+TGG - chr8_4:20464155-20464174 None:intergenic 25.0%
! AAAACACTTTCAATCTAAAG+TGG + chr8_4:20464159-20464178 Msa1318450:intron 25.0%
!!! TTTTGCTGGAAAATTTGTTT+AGG + chr8_4:20464350-20464369 Msa1318450:intron 25.0%
! ATCTATTAATTTCACTGCAT+CGG - chr8_4:20464542-20464561 None:intergenic 25.0%
! TGTTAAGTTATTTGACTATG+AGG + chr8_4:20464603-20464622 Msa1318450:three_prime_UTR 25.0%
!! ATTGATGAAATTTTACATGC+TGG + chr8_4:20464729-20464748 Msa1318450:three_prime_UTR 25.0%
!! GTCAACCATAATCTACTTTT+TGG + chr8_4:20463507-20463526 Msa1318450:intron 30.0%
AGTGGAATGATAAGTATATG+TGG + chr8_4:20463897-20463916 Msa1318450:intron 30.0%
GTGGAATGATAAGTATATGT+GGG + chr8_4:20463898-20463917 Msa1318450:intron 30.0%
! AATACTAGCTAATGGACATT+AGG - chr8_4:20463943-20463962 None:intergenic 30.0%
! TAGAGTAGGTTTAAAGGTTT+TGG + chr8_4:20463965-20463984 Msa1318450:intron 30.0%
!!! TTGTATGGTTTTGTTTTTGC+TGG + chr8_4:20464336-20464355 Msa1318450:intron 30.0%
!! GGAAAATTTGTTTAGGTTGA+TGG + chr8_4:20464357-20464376 Msa1318450:intron 30.0%
!! TGGTTGATTTGAGACAAAAT+TGG - chr8_4:20464681-20464700 None:intergenic 30.0%
!! GTCAACCATAATCTACTTTT+TGG + chr8_4:20463507-20463526 Msa1318450:intron 30.0%
AGTGGAATGATAAGTATATG+TGG + chr8_4:20463897-20463916 Msa1318450:intron 30.0%
GTGGAATGATAAGTATATGT+GGG + chr8_4:20463898-20463917 Msa1318450:intron 30.0%
! AATACTAGCTAATGGACATT+AGG - chr8_4:20463943-20463962 None:intergenic 30.0%
! TAGAGTAGGTTTAAAGGTTT+TGG + chr8_4:20463965-20463984 Msa1318450:intron 30.0%
!!! TTGTATGGTTTTGTTTTTGC+TGG + chr8_4:20464336-20464355 Msa1318450:intron 30.0%
!! GGAAAATTTGTTTAGGTTGA+TGG + chr8_4:20464357-20464376 Msa1318450:intron 30.0%
!! TGGTTGATTTGAGACAAAAT+TGG - chr8_4:20464681-20464700 None:intergenic 30.0%
TTCAGGAGAAAAACTTGAGA+AGG + chr8_4:20463110-20463129 Msa1318450:intron 35.0%
GAAGAAAGAAAAGAAGCTTC+AGG + chr8_4:20463134-20463153 Msa1318450:CDS 35.0%
!! CTAACTTTTCAGGGAGATTA+TGG - chr8_4:20463320-20463339 None:intergenic 35.0%
! CAAACACTGCTAACTTTTCA+GGG - chr8_4:20463329-20463348 None:intergenic 35.0%
! TCAAACACTGCTAACTTTTC+AGG - chr8_4:20463330-20463349 None:intergenic 35.0%
ATGAGTAGTAGCCGAAAATT+AGG + chr8_4:20463553-20463572 Msa1318450:intron 35.0%
! ACACTTTCAATCTAAAGTGG+CGG + chr8_4:20464162-20464181 Msa1318450:intron 35.0%
CAACCACACACATAATATGT+AGG - chr8_4:20464201-20464220 None:intergenic 35.0%
ACTGAAAGTTGAGATTAGAG+AGG + chr8_4:20464263-20464282 Msa1318450:intron 35.0%
GTGTGTATCTTGAGGATTAA+TGG + chr8_4:20464313-20464332 Msa1318450:intron 35.0%
CTTGAGGATTAATGGTTGTA+TGG + chr8_4:20464321-20464340 Msa1318450:intron 35.0%
! TGGTAGTGACAAGAAAAAGA+AGG + chr8_4:20464377-20464396 Msa1318450:CDS 35.0%
GGTAGTGACAAGAAAAAGAA+GGG + chr8_4:20464378-20464397 Msa1318450:CDS 35.0%
GAAAGAAATATCTGAAGACC+AGG + chr8_4:20464424-20464443 Msa1318450:CDS 35.0%
CTTAAAATGGTATCCCTTTC+CGG - chr8_4:20464787-20464806 None:intergenic 35.0%
AGGACTCGTTATCCTTAAAA+TGG - chr8_4:20464800-20464819 None:intergenic 35.0%
AAACCAAACGTGTGTTACAT+TGG - chr8_4:20464862-20464881 None:intergenic 35.0%
AATGTAACACACGTTTGGTT+TGG + chr8_4:20464861-20464880 Msa1318450:three_prime_UTR 35.0%
TTCAGGAGAAAAACTTGAGA+AGG + chr8_4:20463110-20463129 Msa1318450:intron 35.0%
GAAGAAAGAAAAGAAGCTTC+AGG + chr8_4:20463134-20463153 Msa1318450:CDS 35.0%
!! CTAACTTTTCAGGGAGATTA+TGG - chr8_4:20463320-20463339 None:intergenic 35.0%
! CAAACACTGCTAACTTTTCA+GGG - chr8_4:20463329-20463348 None:intergenic 35.0%
! TCAAACACTGCTAACTTTTC+AGG - chr8_4:20463330-20463349 None:intergenic 35.0%
ATGAGTAGTAGCCGAAAATT+AGG + chr8_4:20463553-20463572 Msa1318450:intron 35.0%
! ACACTTTCAATCTAAAGTGG+CGG + chr8_4:20464162-20464181 Msa1318450:intron 35.0%
CAACCACACACATAATATGT+AGG - chr8_4:20464201-20464220 None:intergenic 35.0%
ACTGAAAGTTGAGATTAGAG+AGG + chr8_4:20464263-20464282 Msa1318450:intron 35.0%
GTGTGTATCTTGAGGATTAA+TGG + chr8_4:20464313-20464332 Msa1318450:intron 35.0%
CTTGAGGATTAATGGTTGTA+TGG + chr8_4:20464321-20464340 Msa1318450:intron 35.0%
! TGGTAGTGACAAGAAAAAGA+AGG + chr8_4:20464377-20464396 Msa1318450:CDS 35.0%
GGTAGTGACAAGAAAAAGAA+GGG + chr8_4:20464378-20464397 Msa1318450:CDS 35.0%
GAAAGAAATATCTGAAGACC+AGG + chr8_4:20464424-20464443 Msa1318450:CDS 35.0%
CTTAAAATGGTATCCCTTTC+CGG - chr8_4:20464787-20464806 None:intergenic 35.0%
AGGACTCGTTATCCTTAAAA+TGG - chr8_4:20464800-20464819 None:intergenic 35.0%
AAACCAAACGTGTGTTACAT+TGG - chr8_4:20464862-20464881 None:intergenic 35.0%
AATGTAACACACGTTTGGTT+TGG + chr8_4:20464861-20464880 Msa1318450:three_prime_UTR 35.0%
! GTTGTCTTTTTCTCTTAGCG+AGG - chr8_4:20462985-20463004 None:intergenic 40.0%
! GTTGTCCAAGTAAAGGTACA+GGG - chr8_4:20463992-20464011 None:intergenic 40.0%
! AGTTGTCCAAGTAAAGGTAC+AGG - chr8_4:20463993-20464012 None:intergenic 40.0%
ACTCACAGTTGTCCAAGTAA+AGG - chr8_4:20463999-20464018 None:intergenic 40.0%
TAAACAGATAACCTGGTCTG+TGG - chr8_4:20464047-20464066 None:intergenic 40.0%
TCCTTGCACAAGGAATCATT+GGG - chr8_4:20464098-20464117 None:intergenic 40.0%
GTGCCTACATATTATGTGTG+TGG + chr8_4:20464195-20464214 Msa1318450:intron 40.0%
TGTGGTTGTGCAATAAAAGC+TGG + chr8_4:20464213-20464232 Msa1318450:intron 40.0%
GAGTGTTTGTGTGTATCTTG+AGG + chr8_4:20464305-20464324 Msa1318450:intron 40.0%
AGTGACAAGAAAAAGAAGGG+TGG + chr8_4:20464381-20464400 Msa1318450:CDS 40.0%
! ACAAATAAGGCGTCATTGTG+AGG + chr8_4:20464493-20464512 Msa1318450:exon 40.0%
! TAATTTCACTGCATCGGCAT+CGG - chr8_4:20464536-20464555 None:intergenic 40.0%
TAGATGACATAGCTGTTCTG+AGG + chr8_4:20464557-20464576 Msa1318450:three_prime_UTR 40.0%
CACAGAATGAAATTCACCAC+CGG + chr8_4:20464765-20464784 Msa1318450:three_prime_UTR 40.0%
AATGAAATTCACCACCGGAA+AGG + chr8_4:20464770-20464789 Msa1318450:three_prime_UTR 40.0%
ATGAAATTCACCACCGGAAA+GGG + chr8_4:20464771-20464790 Msa1318450:three_prime_UTR 40.0%
! CGGAAAGGGATACCATTTTA+AGG + chr8_4:20464785-20464804 Msa1318450:three_prime_UTR 40.0%
! AGGATAACGAGTCCTTTTTC+TGG + chr8_4:20464805-20464824 Msa1318450:three_prime_UTR 40.0%
AGTCCAATGTAACACACGTT+TGG + chr8_4:20464856-20464875 Msa1318450:three_prime_UTR 40.0%
! GTTGTCTTTTTCTCTTAGCG+AGG - chr8_4:20462985-20463004 None:intergenic 40.0%
! GTTGTCCAAGTAAAGGTACA+GGG - chr8_4:20463992-20464011 None:intergenic 40.0%
! AGTTGTCCAAGTAAAGGTAC+AGG - chr8_4:20463993-20464012 None:intergenic 40.0%
ACTCACAGTTGTCCAAGTAA+AGG - chr8_4:20463999-20464018 None:intergenic 40.0%
TAAACAGATAACCTGGTCTG+TGG - chr8_4:20464047-20464066 None:intergenic 40.0%
TCCTTGCACAAGGAATCATT+GGG - chr8_4:20464098-20464117 None:intergenic 40.0%
GTGCCTACATATTATGTGTG+TGG + chr8_4:20464195-20464214 Msa1318450:intron 40.0%
TGTGGTTGTGCAATAAAAGC+TGG + chr8_4:20464213-20464232 Msa1318450:intron 40.0%
GAGTGTTTGTGTGTATCTTG+AGG + chr8_4:20464305-20464324 Msa1318450:intron 40.0%
AGTGACAAGAAAAAGAAGGG+TGG + chr8_4:20464381-20464400 Msa1318450:CDS 40.0%
! ACAAATAAGGCGTCATTGTG+AGG + chr8_4:20464493-20464512 Msa1318450:exon 40.0%
! TAATTTCACTGCATCGGCAT+CGG - chr8_4:20464536-20464555 None:intergenic 40.0%
TAGATGACATAGCTGTTCTG+AGG + chr8_4:20464557-20464576 Msa1318450:three_prime_UTR 40.0%
CACAGAATGAAATTCACCAC+CGG + chr8_4:20464765-20464784 Msa1318450:three_prime_UTR 40.0%
AATGAAATTCACCACCGGAA+AGG + chr8_4:20464770-20464789 Msa1318450:three_prime_UTR 40.0%
ATGAAATTCACCACCGGAAA+GGG + chr8_4:20464771-20464790 Msa1318450:three_prime_UTR 40.0%
! CGGAAAGGGATACCATTTTA+AGG + chr8_4:20464785-20464804 Msa1318450:three_prime_UTR 40.0%
! AGGATAACGAGTCCTTTTTC+TGG + chr8_4:20464805-20464824 Msa1318450:three_prime_UTR 40.0%
AGTCCAATGTAACACACGTT+TGG + chr8_4:20464856-20464875 Msa1318450:three_prime_UTR 40.0%
! TCGAAGGTGAGAGTGTACTT+CGG - chr8_4:20463021-20463040 None:intergenic 45.0%
GAAATTGGGGGCAAAATCGA+AGG - chr8_4:20463037-20463056 None:intergenic 45.0%
!! TGGCAGGATTAGAAAGTGTC+TGG - chr8_4:20463747-20463766 None:intergenic 45.0%
CATTGAATGGCCTCTGAGTA+GGG + chr8_4:20463790-20463809 Msa1318450:intron 45.0%
CTTCTTCTTTGGGCGAATGA+GGG + chr8_4:20463832-20463851 Msa1318450:intron 45.0%
TTGGTCCCTGTACCTTTACT+TGG + chr8_4:20463984-20464003 Msa1318450:intron 45.0%
ACTAATCTCTCAAGGGACCA+GGG - chr8_4:20464023-20464042 None:intergenic 45.0%
CTGTGGCACTAATCTCTCAA+GGG - chr8_4:20464030-20464049 None:intergenic 45.0%
TCTGTGGCACTAATCTCTCA+AGG - chr8_4:20464031-20464050 None:intergenic 45.0%
CCAAGCGTAAACAGATAACC+TGG - chr8_4:20464054-20464073 None:intergenic 45.0%
CCAGGTTATCTGTTTACGCT+TGG + chr8_4:20464051-20464070 Msa1318450:intron 45.0%
CCTTGCACAAGGAATCATTG+GGG - chr8_4:20464097-20464116 None:intergenic 45.0%
CTCCTTGCACAAGGAATCAT+TGG - chr8_4:20464099-20464118 None:intergenic 45.0%
GACCACAAGTATGTCCCAAA+GGG - chr8_4:20464122-20464141 None:intergenic 45.0%
TGACCACAAGTATGTCCCAA+AGG - chr8_4:20464123-20464142 None:intergenic 45.0%
! TTTGGGACATACTTGTGGTC+AGG + chr8_4:20464122-20464141 Msa1318450:intron 45.0%
CTAAAGTGGCGGTGATTCAT+AGG + chr8_4:20464173-20464192 Msa1318450:intron 45.0%
GACAAGAAAAAGAAGGGTGG+TGG + chr8_4:20464384-20464403 Msa1318450:CDS 45.0%
!! AAAAAGAAGGGTGGTGGAAG+TGG + chr8_4:20464390-20464409 Msa1318450:CDS 45.0%
! TGGTGGAAGTGGATTTAAGG+TGG + chr8_4:20464401-20464420 Msa1318450:CDS 45.0%
! GGTGGAAGTGGATTTAAGGT+GGG + chr8_4:20464402-20464421 Msa1318450:CDS 45.0%
GCAATGGAGCTCGACAAATA+AGG + chr8_4:20464480-20464499 Msa1318450:CDS 45.0%
AAAATGGTATCCCTTTCCGG+TGG - chr8_4:20464784-20464803 None:intergenic 45.0%
CTAATCAGACGGCCAGAAAA+AGG - chr8_4:20464820-20464839 None:intergenic 45.0%
! TCGAAGGTGAGAGTGTACTT+CGG - chr8_4:20463021-20463040 None:intergenic 45.0%
GAAATTGGGGGCAAAATCGA+AGG - chr8_4:20463037-20463056 None:intergenic 45.0%
!! TGGCAGGATTAGAAAGTGTC+TGG - chr8_4:20463747-20463766 None:intergenic 45.0%
CATTGAATGGCCTCTGAGTA+GGG + chr8_4:20463790-20463809 Msa1318450:intron 45.0%
CTTCTTCTTTGGGCGAATGA+GGG + chr8_4:20463832-20463851 Msa1318450:intron 45.0%
TTGGTCCCTGTACCTTTACT+TGG + chr8_4:20463984-20464003 Msa1318450:intron 45.0%
ACTAATCTCTCAAGGGACCA+GGG - chr8_4:20464023-20464042 None:intergenic 45.0%
CTGTGGCACTAATCTCTCAA+GGG - chr8_4:20464030-20464049 None:intergenic 45.0%
TCTGTGGCACTAATCTCTCA+AGG - chr8_4:20464031-20464050 None:intergenic 45.0%
CCAAGCGTAAACAGATAACC+TGG - chr8_4:20464054-20464073 None:intergenic 45.0%
CCAGGTTATCTGTTTACGCT+TGG + chr8_4:20464051-20464070 Msa1318450:intron 45.0%
CCTTGCACAAGGAATCATTG+GGG - chr8_4:20464097-20464116 None:intergenic 45.0%
CTCCTTGCACAAGGAATCAT+TGG - chr8_4:20464099-20464118 None:intergenic 45.0%
GACCACAAGTATGTCCCAAA+GGG - chr8_4:20464122-20464141 None:intergenic 45.0%
TGACCACAAGTATGTCCCAA+AGG - chr8_4:20464123-20464142 None:intergenic 45.0%
! TTTGGGACATACTTGTGGTC+AGG + chr8_4:20464122-20464141 Msa1318450:intron 45.0%
CTAAAGTGGCGGTGATTCAT+AGG + chr8_4:20464173-20464192 Msa1318450:intron 45.0%
GACAAGAAAAAGAAGGGTGG+TGG + chr8_4:20464384-20464403 Msa1318450:CDS 45.0%
!! AAAAAGAAGGGTGGTGGAAG+TGG + chr8_4:20464390-20464409 Msa1318450:CDS 45.0%
! TGGTGGAAGTGGATTTAAGG+TGG + chr8_4:20464401-20464420 Msa1318450:CDS 45.0%
! GGTGGAAGTGGATTTAAGGT+GGG + chr8_4:20464402-20464421 Msa1318450:CDS 45.0%
GCAATGGAGCTCGACAAATA+AGG + chr8_4:20464480-20464499 Msa1318450:CDS 45.0%
AAAATGGTATCCCTTTCCGG+TGG - chr8_4:20464784-20464803 None:intergenic 45.0%
CTAATCAGACGGCCAGAAAA+AGG - chr8_4:20464820-20464839 None:intergenic 45.0%
CTGCCAGACTTGAGATCCTT+GGG + chr8_4:20463761-20463780 Msa1318450:intron 50.0%
ACACCCAAGGATCTCAAGTC+TGG - chr8_4:20463767-20463786 None:intergenic 50.0%
GACTTGAGATCCTTGGGTGT+TGG + chr8_4:20463767-20463786 Msa1318450:intron 50.0%
CCATTCAATGCCAACACCCA+AGG - chr8_4:20463780-20463799 None:intergenic 50.0%
!! CCTTGGGTGTTGGCATTGAA+TGG + chr8_4:20463777-20463796 Msa1318450:intron 50.0%
GCATTGAATGGCCTCTGAGT+AGG + chr8_4:20463789-20463808 Msa1318450:intron 50.0%
AAGAAGAAGGCTGCAGTCGA+CGG - chr8_4:20463821-20463840 None:intergenic 50.0%
TCGACTGCAGCCTTCTTCTT+TGG + chr8_4:20463821-20463840 Msa1318450:intron 50.0%
CGACTGCAGCCTTCTTCTTT+GGG + chr8_4:20463822-20463841 Msa1318450:intron 50.0%
CCTCATTCGCCCAAAGAAGA+AGG - chr8_4:20463834-20463853 None:intergenic 50.0%
CCTTCTTCTTTGGGCGAATG+AGG + chr8_4:20463831-20463850 Msa1318450:intron 50.0%
CACTAATCTCTCAAGGGACC+AGG - chr8_4:20464024-20464043 None:intergenic 50.0%
AGAGATTAGTGCCACAGACC+AGG + chr8_4:20464033-20464052 Msa1318450:intron 50.0%
TTTACGCTTGGCCTCATGTG+CGG + chr8_4:20464063-20464082 Msa1318450:intron 50.0%
CCCCAATGATTCCTTGTGCA+AGG + chr8_4:20464094-20464113 Msa1318450:intron 50.0%
!! AGCCCTTTGGGACATACTTG+TGG + chr8_4:20464117-20464136 Msa1318450:intron 50.0%
ACATACTTGTGGTCAGGCGT+TGG + chr8_4:20464128-20464147 Msa1318450:intron 50.0%
!! GGGTGGTGGAAGTGGATTTA+AGG + chr8_4:20464398-20464417 Msa1318450:CDS 50.0%
!! CGCAGCTTTAGCTTTCGACA+GGG - chr8_4:20464457-20464476 None:intergenic 50.0%
CTGCCAGACTTGAGATCCTT+GGG + chr8_4:20463761-20463780 Msa1318450:intron 50.0%
ACACCCAAGGATCTCAAGTC+TGG - chr8_4:20463767-20463786 None:intergenic 50.0%
GACTTGAGATCCTTGGGTGT+TGG + chr8_4:20463767-20463786 Msa1318450:intron 50.0%
CCATTCAATGCCAACACCCA+AGG - chr8_4:20463780-20463799 None:intergenic 50.0%
!! CCTTGGGTGTTGGCATTGAA+TGG + chr8_4:20463777-20463796 Msa1318450:intron 50.0%
GCATTGAATGGCCTCTGAGT+AGG + chr8_4:20463789-20463808 Msa1318450:intron 50.0%
AAGAAGAAGGCTGCAGTCGA+CGG - chr8_4:20463821-20463840 None:intergenic 50.0%
TCGACTGCAGCCTTCTTCTT+TGG + chr8_4:20463821-20463840 Msa1318450:intron 50.0%
CGACTGCAGCCTTCTTCTTT+GGG + chr8_4:20463822-20463841 Msa1318450:intron 50.0%
CCTCATTCGCCCAAAGAAGA+AGG - chr8_4:20463834-20463853 None:intergenic 50.0%
CCTTCTTCTTTGGGCGAATG+AGG + chr8_4:20463831-20463850 Msa1318450:intron 50.0%
CACTAATCTCTCAAGGGACC+AGG - chr8_4:20464024-20464043 None:intergenic 50.0%
AGAGATTAGTGCCACAGACC+AGG + chr8_4:20464033-20464052 Msa1318450:intron 50.0%
TTTACGCTTGGCCTCATGTG+CGG + chr8_4:20464063-20464082 Msa1318450:intron 50.0%
CCCCAATGATTCCTTGTGCA+AGG + chr8_4:20464094-20464113 Msa1318450:intron 50.0%
!! AGCCCTTTGGGACATACTTG+TGG + chr8_4:20464117-20464136 Msa1318450:intron 50.0%
ACATACTTGTGGTCAGGCGT+TGG + chr8_4:20464128-20464147 Msa1318450:intron 50.0%
!! GGGTGGTGGAAGTGGATTTA+AGG + chr8_4:20464398-20464417 Msa1318450:CDS 50.0%
!! CGCAGCTTTAGCTTTCGACA+GGG - chr8_4:20464457-20464476 None:intergenic 50.0%
CCAAGGATCTCAAGTCTGGC+AGG - chr8_4:20463763-20463782 None:intergenic 55.0%
CCTGCCAGACTTGAGATCCT+TGG + chr8_4:20463760-20463779 Msa1318450:intron 55.0%
GCGCGTGCTAACTAGACTGT+TGG - chr8_4:20463877-20463896 None:intergenic 55.0%
AGTCTAGTTAGCACGCGCAG+TGG + chr8_4:20463879-20463898 Msa1318450:intron 55.0%
TTGGACAACTGTGAGTGCCC+TGG + chr8_4:20464003-20464022 Msa1318450:intron 55.0%
GGGTTGTGATACCGCACATG+AGG - chr8_4:20464077-20464096 None:intergenic 55.0%
TCCTTGTGCAAGGAGCCCTT+TGG + chr8_4:20464104-20464123 Msa1318450:intron 55.0%
CCTTGTGCAAGGAGCCCTTT+GGG + chr8_4:20464105-20464124 Msa1318450:intron 55.0%
TTTCGACAGGGCTGACAACC+TGG - chr8_4:20464445-20464464 None:intergenic 55.0%
!! GCGCAGCTTTAGCTTTCGAC+AGG - chr8_4:20464458-20464477 None:intergenic 55.0%
GTCGAAAGCTAAAGCTGCGC+AGG + chr8_4:20464458-20464477 Msa1318450:CDS 55.0%
AGCTAAAGCTGCGCAGGCAA+TGG + chr8_4:20464464-20464483 Msa1318450:CDS 55.0%
CCAAGGATCTCAAGTCTGGC+AGG - chr8_4:20463763-20463782 None:intergenic 55.0%
CCTGCCAGACTTGAGATCCT+TGG + chr8_4:20463760-20463779 Msa1318450:intron 55.0%
GCGCGTGCTAACTAGACTGT+TGG - chr8_4:20463877-20463896 None:intergenic 55.0%
AGTCTAGTTAGCACGCGCAG+TGG + chr8_4:20463879-20463898 Msa1318450:intron 55.0%
TTGGACAACTGTGAGTGCCC+TGG + chr8_4:20464003-20464022 Msa1318450:intron 55.0%
GGGTTGTGATACCGCACATG+AGG - chr8_4:20464077-20464096 None:intergenic 55.0%
TCCTTGTGCAAGGAGCCCTT+TGG + chr8_4:20464104-20464123 Msa1318450:intron 55.0%
CCTTGTGCAAGGAGCCCTTT+GGG + chr8_4:20464105-20464124 Msa1318450:intron 55.0%
TTTCGACAGGGCTGACAACC+TGG - chr8_4:20464445-20464464 None:intergenic 55.0%
!! GCGCAGCTTTAGCTTTCGAC+AGG - chr8_4:20464458-20464477 None:intergenic 55.0%
GTCGAAAGCTAAAGCTGCGC+AGG + chr8_4:20464458-20464477 Msa1318450:CDS 55.0%
AGCTAAAGCTGCGCAGGCAA+TGG + chr8_4:20464464-20464483 Msa1318450:CDS 55.0%
AAGAAGGCTGCAGTCGACGG+AGG - chr8_4:20463818-20463837 None:intergenic 60.0%
CCCAAAGGGCTCCTTGCACA+AGG - chr8_4:20464108-20464127 None:intergenic 60.0%
AAGAAGGCTGCAGTCGACGG+AGG - chr8_4:20463818-20463837 None:intergenic 60.0%
CCCAAAGGGCTCCTTGCACA+AGG - chr8_4:20464108-20464127 None:intergenic 60.0%
!! GACGGAGGTGCCCTACTCAG+AGG - chr8_4:20463803-20463822 None:intergenic 65.0%
!! GACGGAGGTGCCCTACTCAG+AGG - chr8_4:20463803-20463822 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 20462899 20464913 20462899 ID=Msa1318450;Name=Msa1318450
chr8_4 mRNA 20462899 20464913 20462899 ID=Msa1318450-mRNA-1;Parent=Msa1318450;Name=Msa1318450-mRNA-1;_AED=0.00;_eAED=0.00;_QI=66|1|1|1|1|1|3|413|82
chr8_4 exon 20462899 20463022 20462899 ID=Msa1318450-mRNA-1:exon:7492;Parent=Msa1318450-mRNA-1
chr8_4 exon 20463115 20463176 20463115 ID=Msa1318450-mRNA-1:exon:7493;Parent=Msa1318450-mRNA-1
chr8_4 exon 20464372 20464913 20464372 ID=Msa1318450-mRNA-1:exon:7494;Parent=Msa1318450-mRNA-1
chr8_4 five_prime_UTR 20462899 20462964 20462899 ID=Msa1318450-mRNA-1:five_prime_utr;Parent=Msa1318450-mRNA-1
chr8_4 CDS 20462965 20463022 20462965 ID=Msa1318450-mRNA-1:cds;Parent=Msa1318450-mRNA-1
chr8_4 CDS 20463115 20463176 20463115 ID=Msa1318450-mRNA-1:cds;Parent=Msa1318450-mRNA-1
chr8_4 CDS 20464372 20464500 20464372 ID=Msa1318450-mRNA-1:cds;Parent=Msa1318450-mRNA-1
chr8_4 three_prime_UTR 20464501 20464913 20464501 ID=Msa1318450-mRNA-1:three_prime_utr;Parent=Msa1318450-mRNA-1
Gene Sequence

>Msa1318450

ATGTCGAAGAAGAACGACCTCGCTAAGAGAAAAAGACAACACGAATTCGAACTCCGAAGAGAAAAACTTGAGAAGGAGAAGAAAGAAAAGAAGCTTCAGGCGAAGAAAAACAAGATGAAAGTTGATGGTAGTGACAAGAAAAAGAAGGGTGGTGGAAGTGGATTTAAGGTGGGAAAGAAATATCTGAAGACCAGGTTGTCAGCCCTGTCGAAAGCTAAAGCTGCGCAGGCAATGGAGCTCGACAAATAA
Protein sequence

>Msa1318450

MSKKNDLAKRKRQHEFELRREKLEKEKKEKKLQAKKNKMKVDGSDKKKKGGGSGFKVGKKYLKTRLSALSKAKAAQAMELDK*