AlfalfaGEDB | Msa1320630

M. sativa cultivar zhongmu-4 / Msa1320630

KEGG: K08064
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0006355 GO:0007275 GO:0008150 GO:0009555 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009892 GO:0010154 GO:0010262 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0045892 GO:0045934 GO:0048229 GO:0048316 GO:0048519 GO:0048523 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0055046 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0140110 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
PFAM: PF02045.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1320630	sp\|Q945M9\|NFYA9_ARATH	50.340	147	70	2	98	241	108	254	9.30e-40	144

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1320630	I3TAV7	94.408	304	16	1	1	303	1	304	0.0	591

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TFs/TRs:

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Gene ID	Type	Classification
Msa1320630	TF	NF-YA

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1185990	Msa1320630	0.897885	9.103651e-77	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1320630	MtrunA17_Chr8g0352251	94.158	291	16	1	1	290	1	291	0.0	564
Msa1320630	MtrunA17_Chr3g0103391	36.991	319	140	11	21	302	20	314	1.20e-45	157
Msa1320630	MtrunA17_Chr2g0292411	71.111	90	26	0	127	216	86	175	1.77e-36	130
Msa1320630	MtrunA17_Chr8g0343451	43.415	205	92	9	81	282	95	278	1.22e-34	128
Msa1320630	MtrunA17_Chr2g0300261	48.000	150	70	4	114	258	33	179	4.45e-34	124
Msa1320630	MtrunA17_Chr2g0329811	51.613	124	53	2	117	233	144	267	1.76e-31	120
Msa1320630	MtrunA17_Chr7g0267581	45.752	153	58	6	72	219	99	231	2.45e-30	117
Msa1320630	MtrunA17_Chr1g0177091	54.206	107	36	3	109	213	128	223	3.13e-28	111

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1320630	AT3G20910.1	50.340	147	70	2	98	241	108	254	9.45e-41	144
Msa1320630	AT5G12840.4	40.000	240	103	8	29	238	27	255	6.52e-40	141
Msa1320630	AT5G12840.2	40.000	240	103	8	29	238	27	255	6.52e-40	141
Msa1320630	AT5G12840.5	51.095	137	66	1	103	238	120	256	7.59e-40	141
Msa1320630	AT5G12840.3	51.095	137	66	1	103	238	120	256	7.59e-40	141
Msa1320630	AT5G12840.1	51.095	137	66	1	103	238	120	256	7.59e-40	141
Msa1320630	AT1G30500.2	43.976	166	77	4	79	236	33	190	1.27e-32	120
Msa1320630	AT1G30500.1	43.373	166	74	4	79	236	33	186	1.25e-31	117
Msa1320630	AT2G34720.1	37.949	195	98	5	66	252	8	187	2.11e-31	117
Msa1320630	AT1G72830.1	53.448	116	49	3	115	225	137	252	9.96e-31	119

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Find 108 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCTGTGAAAGGATGCC+AGG	0.439397	8_4:+25296659	Msa1320630:exon
AAACAGGAGGGTGACTTCTT+TGG	0.313426	8_4:+25299717	Msa1320630:CDS
AAAGCTGTGAAAGGATGCCA+GGG	0.629084	8_4:+25296660	Msa1320630:exon
AACAATACTGGTGCAACATC+GGG	0.490937	8_4:+25299570	Msa1320630:CDS
AACATGCTTTGAGAAGGGCA+AGG	0.629479	8_4:+25299478	Msa1320630:CDS
AAGTAAGGGGAGAACGAACG+AGG	0.701281	8_4:-25300007	None:intergenic
AATTCGCACACGGAACTTGT+TGG	0.449001	8_4:+25297463	Msa1320630:CDS
ACAAGTTCCGTGTGCGAATT+CGG	0.296355	8_4:-25297460	None:intergenic
ACAGCATCATGAGTTGGATG+AGG	0.597562	8_4:-25299909	None:intergenic
ACAGGCAAATGGAAAACAGG+AGG	0.631076	8_4:+25299704	Msa1320630:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGACAAAGCTCATATTGTT+AGG	+	chr8_4:25295527-25295546	Msa1320630:five_prime_UTR	30.0%
TAGAAGAACTTACTATTGGA+AGG	-	chr8_4:25296057-25296076	None:intergenic	30.0%
AAACACCCTTTACAAAATTG+TGG	-	chr8_4:25296137-25296156	None:intergenic	30.0%
AGCTTATCTAAGTAAAAGCA+CGG	+	chr8_4:25296376-25296395	Msa1320630:intron	30.0%
CTTTCTTTAACTGATAACTC+AGG	+	chr8_4:25296863-25296882	Msa1320630:CDS	30.0%
TCTAGATTTGTCAAACCAAT+TGG	+	chr8_4:25297038-25297057	Msa1320630:intron	30.0%
ATCATGATGTGTACATATCT+TGG	-	chr8_4:25297524-25297543	None:intergenic	30.0%
CCAAATTGTGCATTTGAATA+AGG	-	chr8_4:25297584-25297603	None:intergenic	30.0%
CCTTATTCAAATGCACAATT+TGG	+	chr8_4:25297581-25297600	Msa1320630:CDS	30.0%
CTTGCCAATGAATAAAAAGA+AGG	-	chr8_4:25297730-25297749	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	25294733	25300226	25294733	ID=Msa1320630;Name=Msa1320630
chr8_4	mRNA	25294733	25300226	25294733	ID=Msa1320630-mRNA-1;Parent=Msa1320630;Name=Msa1320630-mRNA-1;_AED=0.13;_eAED=0.13;_QI=1348\|0.83\|0.85\|1\|0.83\|0.71\|7\|245\|319
chr8_4	exon	25294733	25296063	25294733	ID=Msa1320630-mRNA-1:exon:8676;Parent=Msa1320630-mRNA-1
chr8_4	exon	25296657	25296884	25296657	ID=Msa1320630-mRNA-1:exon:8677;Parent=Msa1320630-mRNA-1
chr8_4	exon	25297380	25297495	25297380	ID=Msa1320630-mRNA-1:exon:8678;Parent=Msa1320630-mRNA-1
chr8_4	exon	25297569	25297637	25297569	ID=Msa1320630-mRNA-1:exon:8679;Parent=Msa1320630-mRNA-1
chr8_4	exon	25298906	25299070	25298906	ID=Msa1320630-mRNA-1:exon:8680;Parent=Msa1320630-mRNA-1
chr8_4	exon	25299453	25299759	25299453	ID=Msa1320630-mRNA-1:exon:8681;Parent=Msa1320630-mRNA-1
chr8_4	exon	25299890	25300226	25299890	ID=Msa1320630-mRNA-1:exon:8682;Parent=Msa1320630-mRNA-1
chr8_4	five_prime_UTR	25294733	25296063	25294733	ID=Msa1320630-mRNA-1:five_prime_utr;Parent=Msa1320630-mRNA-1
chr8_4	five_prime_UTR	25296657	25296673	25296657	ID=Msa1320630-mRNA-1:five_prime_utr;Parent=Msa1320630-mRNA-1
chr8_4	CDS	25296674	25296884	25296674	ID=Msa1320630-mRNA-1:cds;Parent=Msa1320630-mRNA-1
chr8_4	CDS	25297380	25297495	25297380	ID=Msa1320630-mRNA-1:cds;Parent=Msa1320630-mRNA-1
chr8_4	CDS	25297569	25297637	25297569	ID=Msa1320630-mRNA-1:cds;Parent=Msa1320630-mRNA-1
chr8_4	CDS	25298906	25299070	25298906	ID=Msa1320630-mRNA-1:cds;Parent=Msa1320630-mRNA-1
chr8_4	CDS	25299453	25299759	25299453	ID=Msa1320630-mRNA-1:cds;Parent=Msa1320630-mRNA-1
chr8_4	CDS	25299890	25299981	25299890	ID=Msa1320630-mRNA-1:cds;Parent=Msa1320630-mRNA-1
chr8_4	three_prime_UTR	25299982	25300226	25299982	ID=Msa1320630-mRNA-1:three_prime_utr;Parent=Msa1320630-mRNA-1

Gene Sequence

>Msa1320630

ATGCCAGGGAAACCAGAAACTGATGATTGGAGAATAAAGCAAAGTGAGCAGGTTCAGTTGCCATCTTCCATTTACTCTCATCATCAGCCTTGGTGGCTTGAAAATGGCTCCAAACCATCTTCAGCTGATCAGATAAATGGTTCAATCATGAATAATGTCACACATTCAGAGCCTTTGGTTTCTCCTTCACTTTCTTTAACTGATAACTCAGGTGGGGACGTTGCCAAGGAACACCGAAACATCAAACAGGCCCTGTCCTCAACCACATTTACCATGGATAAGCACCTTGATCCGAATTCGCACACGGAACTTGTTGGTCATTCGATTGTTTTAACATCTCCTTATTCAAATGCACAATTTGGTCAAATCTTGAATGCTTATGGACAACAAGCTATGATGAATCATCAGCTATACCAAATGCATCATGCTAGAATGCTTTTGCCGCTTAAAATGGAAGAGGAACCTGTGTATGTCAATGCAAAGCAGTATCATGGTATTTTGAGACGAAGACAGTCGCGCGCTAAGGCTGAGCTTGAAAAGAAAGTTATTAAAGTTAGAAAGCCATATCTTCATGAATCCCGTCACCAACATGCTTTGAGAAGGGCAAGGGGAAATGGCGGTCGTTTTCTCAATACAAAGAAGCTCGAACACAACGATTCTGCTGCTCTAGAAAAAGGCAACAATACTGGTGCAACATCGGGTACCAACAATGAGAATCAAGGCTCATCTAATGTGTCGCAGCACATGCACAAAATGCAGGGTTTCAACATCAGTTACCATGATGGAAATGGCTTTACAGCACTATGTCATTCACAGGCAAATGGAAAACAGGAGGGTGACTTCTTTGGTAAAAAGATTGAATCAAATGGTGAAGATGAAGGGCTTTGCCTCATCCAACTCATGATGCTGTATATCAATGGCAACTCATTCTTGGCTAAGTTAGTTAGACTACAGTGGTGA

Protein sequence

>Msa1320630

MPGKPETDDWRIKQSEQVQLPSSIYSHHQPWWLENGSKPSSADQINGSIMNNVTHSEPLVSPSLSLTDNSGGDVAKEHRNIKQALSSTTFTMDKHLDPNSHTELVGHSIVLTSPYSNAQFGQILNAYGQQAMMNHQLYQMHHARMLLPLKMEEEPVYVNAKQYHGILRRRQSRAKAELEKKVIKVRKPYLHESRHQHALRRARGNGGRFLNTKKLEHNDSAALEKGNNTGATSGTNNENQGSSNVSQHMHKMQGFNISYHDGNGFTALCHSQANGKQEGDFFGKKIESNGEDEGLCLIQLMMLYINGNSFLAKLVRLQW*