AlfalfaGEDB | Msa1320750

M. sativa cultivar zhongmu-4 / Msa1320750

KEGG: K10685
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005911 GO:0006464 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008641 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016874 GO:0016877 GO:0016925 GO:0018193 GO:0018205 GO:0019538 GO:0019948 GO:0022414 GO:0030054 GO:0032446 GO:0032501 GO:0032502 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0055044 GO:0061458 GO:0070647 GO:0071704 GO:1901564
PFAM: PF00899.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1320750	sp\|Q9SJT1\|SAE2_ARATH	78.435	524	112	1	4	526	8	531	0.0	879

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1320750	A0A072TPF5	98.898	635	7	0	1	635	1	635	0.0	1295

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0117710	Msa1320750	0.807218	5.626679e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1320750	MtrunA17_Chr8g0352371	98.898	635	7	0	1	635	1	635	0.0	1295
Msa1320750	MtrunA17_Chr4g0074481	41.711	187	92	3	9	180	48	232	1.92e-39	150
Msa1320750	MtrunA17_Chr0c01g0489601	29.139	453	254	14	6	405	210	648	9.47e-37	146
Msa1320750	MtrunA17_Chr5g0431171	40.217	184	102	2	6	181	493	676	8.64e-33	135
Msa1320750	MtrunA17_Chr5g0431151	40.659	182	100	2	8	181	496	677	2.47e-30	127
Msa1320750	MtrunA17_Chr5g0444711	28.105	153	108	2	9	160	91	242	1.43e-19	92.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1320750	AT2G21470.1	72.375	619	153	5	4	621	8	609	0.0	912
Msa1320750	AT2G21470.3	72.026	622	153	6	4	621	8	612	0.0	905
Msa1320750	AT2G21470.2	78.435	524	112	1	4	526	8	531	0.0	879
Msa1320750	AT5G19180.1	37.981	208	111	4	6	197	43	248	3.81e-39	150
Msa1320750	AT5G19180.2	37.981	208	111	4	6	197	43	248	5.20e-39	149
Msa1320750	AT5G06460.1	39.674	184	103	2	6	181	487	670	5.81e-32	133
Msa1320750	AT2G30110.1	40.761	184	101	2	6	181	490	673	1.05e-29	126
Msa1320750	AT5G55130.1	29.560	159	109	3	3	160	87	243	5.15e-18	87.8
Msa1320750	AT1G05350.1	28.235	170	96	4	6	154	80	244	6.06e-14	74.7
Msa1320750	AT5G55130.2	26.950	141	99	3	23	160	77	216	2.37e-12	70.1

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Find 129 sgRNAs with CRISPR-Local

Find 391 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATATTTGATCACGTATT+TGG	0.071426	8_4:+25546821	Msa1320750:CDS
AAAATGACATTAAAAGTTAT+AGG	0.357518	8_4:+25547425	Msa1320750:CDS
AAAATTTATGAGAGAGAAAG+AGG	0.428612	8_4:-25543225	None:intergenic
AAACACTCTGCTTATGCCAG+TGG	0.641266	8_4:+25548528	Msa1320750:CDS
AAACCAACACATACCTTTGA+TGG	0.425453	8_4:-25546275	None:intergenic
AAAGTTCTCATGGTTGGTGC+TGG	0.510374	8_4:+25543409	Msa1320750:CDS
AAATATTTGATCACGTATTT+GGG	0.339346	8_4:+25546822	Msa1320750:CDS
AAATTTATGAGAGAGAAAGA+GGG	0.580793	8_4:-25543224	None:intergenic
AACATACGTGCTGCTTCATT+TGG	0.360112	8_4:+25547283	Msa1320750:CDS
AACCAACACATACCTTTGAT+GGG	0.507387	8_4:-25546274	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTTAGTACATGTTTGGTTG+TGG	+	chr8_4:25544070-25544089	Msa1320750:intron	30.0%
ATTAACCCTGTTATTATTGG+GGG	+	chr8_4:25544191-25544210	Msa1320750:intron	30.0%
CGGTTCAGTTATTAAAACAT+TGG	-	chr8_4:25544223-25544242	None:intergenic	30.0%
ATGGTTCAACAACTTTAACT+CGG	+	chr8_4:25544268-25544287	Msa1320750:intron	30.0%
CAATGATTTAAAAACCAACC+CGG	-	chr8_4:25544379-25544398	None:intergenic	30.0%
ATGATGTACATGGTCTATAT+TGG	-	chr8_4:25544494-25544513	None:intergenic	30.0%
AATGTAATAAAGCACATCCA+TGG	-	chr8_4:25544720-25544739	None:intergenic	30.0%
ACAATTAAATTACCTGACCA+AGG	-	chr8_4:25545144-25545163	None:intergenic	30.0%
TAGATAATCAATCTTCCAAG+TGG	+	chr8_4:25545379-25545398	Msa1320750:intron	30.0%
AGATAATCAATCTTCCAAGT+GGG	+	chr8_4:25545380-25545399	Msa1320750:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	25543216	25549839	25543216	ID=Msa1320750;Name=Msa1320750
chr8_4	mRNA	25543216	25549839	25543216	ID=Msa1320750-mRNA-1;Parent=Msa1320750;Name=Msa1320750-mRNA-1;_AED=0.12;_eAED=0.12;_QI=83\|1\|1\|1\|1\|1\|11\|343\|635
chr8_4	exon	25543216	25543319	25543216	ID=Msa1320750-mRNA-1:exon:8745;Parent=Msa1320750-mRNA-1
chr8_4	exon	25543403	25543492	25543403	ID=Msa1320750-mRNA-1:exon:8746;Parent=Msa1320750-mRNA-1
chr8_4	exon	25544769	25544852	25544769	ID=Msa1320750-mRNA-1:exon:8747;Parent=Msa1320750-mRNA-1
chr8_4	exon	25544932	25545150	25544932	ID=Msa1320750-mRNA-1:exon:8748;Parent=Msa1320750-mRNA-1
chr8_4	exon	25546182	25546283	25546182	ID=Msa1320750-mRNA-1:exon:8749;Parent=Msa1320750-mRNA-1
chr8_4	exon	25546650	25547108	25546650	ID=Msa1320750-mRNA-1:exon:8750;Parent=Msa1320750-mRNA-1
chr8_4	exon	25547217	25547446	25547217	ID=Msa1320750-mRNA-1:exon:8751;Parent=Msa1320750-mRNA-1
chr8_4	exon	25548498	25548591	25548498	ID=Msa1320750-mRNA-1:exon:8752;Parent=Msa1320750-mRNA-1
chr8_4	exon	25548682	25548861	25548682	ID=Msa1320750-mRNA-1:exon:8753;Parent=Msa1320750-mRNA-1
chr8_4	exon	25548932	25549029	25548932	ID=Msa1320750-mRNA-1:exon:8754;Parent=Msa1320750-mRNA-1
chr8_4	exon	25549166	25549839	25549166	ID=Msa1320750-mRNA-1:exon:8755;Parent=Msa1320750-mRNA-1
chr8_4	five_prime_UTR	25543216	25543298	25543216	ID=Msa1320750-mRNA-1:five_prime_utr;Parent=Msa1320750-mRNA-1
chr8_4	CDS	25543299	25543319	25543299	ID=Msa1320750-mRNA-1:cds;Parent=Msa1320750-mRNA-1
chr8_4	CDS	25543403	25543492	25543403	ID=Msa1320750-mRNA-1:cds;Parent=Msa1320750-mRNA-1
chr8_4	CDS	25544769	25544852	25544769	ID=Msa1320750-mRNA-1:cds;Parent=Msa1320750-mRNA-1
chr8_4	CDS	25544932	25545150	25544932	ID=Msa1320750-mRNA-1:cds;Parent=Msa1320750-mRNA-1
chr8_4	CDS	25546182	25546283	25546182	ID=Msa1320750-mRNA-1:cds;Parent=Msa1320750-mRNA-1
chr8_4	CDS	25546650	25547108	25546650	ID=Msa1320750-mRNA-1:cds;Parent=Msa1320750-mRNA-1
chr8_4	CDS	25547217	25547446	25547217	ID=Msa1320750-mRNA-1:cds;Parent=Msa1320750-mRNA-1
chr8_4	CDS	25548498	25548591	25548498	ID=Msa1320750-mRNA-1:cds;Parent=Msa1320750-mRNA-1
chr8_4	CDS	25548682	25548861	25548682	ID=Msa1320750-mRNA-1:cds;Parent=Msa1320750-mRNA-1
chr8_4	CDS	25548932	25549029	25548932	ID=Msa1320750-mRNA-1:cds;Parent=Msa1320750-mRNA-1
chr8_4	CDS	25549166	25549496	25549166	ID=Msa1320750-mRNA-1:cds;Parent=Msa1320750-mRNA-1
chr8_4	three_prime_UTR	25549497	25549839	25549497	ID=Msa1320750-mRNA-1:three_prime_utr;Parent=Msa1320750-mRNA-1

Gene Sequence

>Msa1320750

ATGGCTTCTTCCATTATCAAAGATGCAAAAGTTCTCATGGTTGGTGCTGGTGGAATTGGTTGCGAGCTTCTCAAAACTCTTGCTCTCTCTGGCTTCTCTGACATACACATCATTGACATGGACACTATAGAAGTCAGTAACCTGAACAGACAGTTTTTGTTTCGACAATCTCATGTTGGGCAGTCCAAGGCTAAAGTTGCTAGGGATGCTGTTTTAAAATTTAGGCCCAAGATAAACATTACATCATATCATGCAAATGTCAAAGATCCTGATTTCAATGTGGACTTCTTTAAGCAATTTAATGTTGTTCTTAATGGGCTGGACAACCTAGATGCACGGCGACATGTGAATCGTTTGTGCTTGGCAGCTGATGTTCCTTTAGTTGAAAGTGGAACTACTGGGTTCCTTGGTCAGGTTACAGTTCATGTCAAAGGAAGAACAGAGTGCTATGAGTGTCAGCCTAAACCAGCTCCAAAGACATATCCAGTCTGTACTATCACAAGTACCCCATCAAAGTTTGTTCACTGTATTGTGTGGGCAAAAGAACTACTATTTTCTAAGTTATTTGGCGACAAAAATCAAGACAATGATTTAAATGTTCGCTCAAGCGATGCTGCCAGCTCATCAAAAAACATAGAGGATATATTTGAACATAGCAAAGATGAAGACATCGGTCAATATGGAAAGAAAATATTTGATCACGTATTTGGGTATAATATTGAATTAGCTTTGTCCAATGAGGAGACATGGAAAAATCGAAATAGACCTAAACCTATATACATCAAGGATGTCCTGTCTGATGAATTGGATAAACAGAATGGAAATCTGGATAATGATAATGCTTGTGGCGATGGATTTTCAGTTTCTGCCATGTCATCTTTGGGCATGAAGAACCCGCAAGATATCTGGAGTCTTAGAGAAAATTCTAGAGTATTTCTCGAAGCATTGAGACTATTTTTCACAAAAAGAGAAAAGGATATTGGCAATCTGAGCTTTGATAAAGATGACCAGTTGGCTGTAGAATTTGTTACTGCAGCTGCAAACATACGTGCTGCTTCATTTGGCATACCTTTACATAGTCTTTTTGAAGCTAAAGGAATTGCTGGTAACATTGTGCATGCTGTGGCAACAACAAATGCTGTTATTGCTGGTTTGATTGTCATTGAAGCAATCAAGGTGCTGAAAAATGACATTAAAAGTTATAGAATGACATATTGTCTGGAACATCCAGCAAGAAACACTCTGCTTATGCCAGTGGAGCCATTTGAGCCTAACAAATCATGTTATGTTTGTTCTGAGAGTCCTGTATTGCTTGAAATAAACACAAACCGTTCAAAGTTGAAGGATGTTGTCGAAAAGATCATCAAGGCTAAGCTTGGGATGAGCCTTCCACTTGTTATGAATGCTTCAAACCTCCTCTATGAAGCTGGTGATATTGAGGATGACATGGTTGCAATTTATAATGCCAACCTTGAAAAGGTCCTAGCTGAGCTTCCTTCTCCTGTAACTGGTGGTACAATGCTTACAGTAGAGGATTTTCAACAGGAATTGAAGTGCAACATTAACATAAAGCACAGAGAGGAATTTGATGAAGAGAAAGAACCTGATGGTATGGTACTCTCAGGATGGACTCAACCAGTCGCAGCAGCAGAAAATGAAGACAAATCTGTTAGTAACGGTGCGAACACCTCAGACGCACCAATAACAGCAGTAGAAAATTCTGAAAATGATGATGATGTTGGTATAATTTCTCCGGTGAAGAAAAGAAAGCTTCCTGATGATTCTGATAATTCGAATACTGCTGATGACGAAAGGCATCACAAGAAATTGCAAGTAATCGATGATGAAGATGATCTTGTCATGCTCGATGGGAATTTAGATGGGTTTAAAAAGAGAAGAGTGTCATAG

Protein sequence

>Msa1320750

MASSIIKDAKVLMVGAGGIGCELLKTLALSGFSDIHIIDMDTIEVSNLNRQFLFRQSHVGQSKAKVARDAVLKFRPKINITSYHANVKDPDFNVDFFKQFNVVLNGLDNLDARRHVNRLCLAADVPLVESGTTGFLGQVTVHVKGRTECYECQPKPAPKTYPVCTITSTPSKFVHCIVWAKELLFSKLFGDKNQDNDLNVRSSDAASSSKNIEDIFEHSKDEDIGQYGKKIFDHVFGYNIELALSNEETWKNRNRPKPIYIKDVLSDELDKQNGNLDNDNACGDGFSVSAMSSLGMKNPQDIWSLRENSRVFLEALRLFFTKREKDIGNLSFDKDDQLAVEFVTAAANIRAASFGIPLHSLFEAKGIAGNIVHAVATTNAVIAGLIVIEAIKVLKNDIKSYRMTYCLEHPARNTLLMPVEPFEPNKSCYVCSESPVLLEINTNRSKLKDVVEKIIKAKLGMSLPLVMNASNLLYEAGDIEDDMVAIYNANLEKVLAELPSPVTGGTMLTVEDFQQELKCNINIKHREEFDEEKEPDGMVLSGWTQPVAAAENEDKSVSNGANTSDAPITAVENSENDDDVGIISPVKKRKLPDDSDNSNTADDERHHKKLQVIDDEDDLVMLDGNLDGFKKRRVS*