AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1323140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1323140 MtrunA17_Chr8g0355821 96.104 154 6 0 1 154 1 154 1.52e-109 308
Msa1323140 MtrunA17_Chr8g0355811 90.385 156 15 0 1 156 1 156 8.75e-105 296
Msa1323140 MtrunA17_Chr8g0355801 85.256 156 23 0 1 156 1 156 9.14e-98 278
Msa1323140 MtrunA17_Chr8g0355791 83.333 156 26 0 1 156 1 156 2.13e-94 270
Msa1323140 MtrunA17_Chr8g0355771 82.051 156 28 0 1 156 1 156 2.90e-93 267
Msa1323140 MtrunA17_Chr8g0355741 75.159 157 38 1 1 156 1 157 1.40e-85 247
Msa1323140 MtrunA17_Chr8g0355721 71.975 157 42 2 1 156 1 156 7.63e-82 238
Msa1323140 MtrunA17_Chr8g0355661 71.338 157 43 2 1 156 1 156 1.57e-80 234
Msa1323140 MtrunA17_Chr8g0355781 68.421 152 47 1 1 151 1 152 1.04e-76 225
Msa1323140 MtrunA17_Chr8g0355761 69.079 152 46 1 1 151 1 152 1.06e-76 225
Msa1323140 MtrunA17_Chr8g0355641 66.026 156 52 1 1 155 1 156 8.55e-76 223
Msa1323140 MtrunA17_Chr1g0160371 41.830 153 85 3 1 150 1 152 3.26e-39 130
Msa1323140 MtrunA17_Chr1g0160381 38.562 153 90 3 1 150 5 156 1.90e-37 125
Msa1323140 MtrunA17_Chr1g0160391 37.821 156 88 5 1 148 1 155 3.52e-28 102
Msa1323140 MtrunA17_Chr8g0339261 34.667 150 94 4 6 153 8 155 2.31e-26 97.4
Msa1323140 MtrunA17_Chr1g0160801 37.838 148 82 6 7 151 7 147 3.68e-22 86.3
Msa1323140 MtrunA17_Chr1g0160351 40.860 93 52 2 61 150 1 93 2.34e-20 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1323140 AT5G28010.1 35.948 153 94 4 3 152 15 166 4.26e-26 97.4
Msa1323140 AT5G28010.2 35.948 153 94 4 3 152 15 166 4.26e-26 97.4
Msa1323140 AT1G70890.1 37.255 153 92 4 3 152 7 158 2.30e-24 92.8
Msa1323140 AT1G70840.1 35.948 153 94 4 3 152 20 171 3.91e-24 92.4
Msa1323140 AT1G70830.5 36.538 156 89 5 3 152 22 173 3.03e-23 90.1
Msa1323140 AT1G70830.3 36.306 157 90 5 3 153 22 174 3.24e-23 90.9
Msa1323140 AT5G28000.1 33.987 153 97 4 3 152 13 164 3.86e-23 89.7
Msa1323140 AT1G70830.2 36.306 157 90 5 3 153 22 174 2.46e-22 89.7
Msa1323140 AT1G70830.4 36.538 156 89 5 3 152 137 288 3.83e-22 90.1
Msa1323140 AT1G70830.4 35.052 97 61 2 59 153 31 127 3.16e-12 63.2
Msa1323140 AT1G70830.1 36.306 157 90 5 3 153 22 174 4.54e-22 90.5
Msa1323140 AT1G70830.1 36.538 156 89 5 3 152 184 335 1.14e-21 89.4
Msa1323140 AT1G70850.3 37.671 146 81 5 13 152 175 316 1.43e-21 89.0
Msa1323140 AT1G70850.3 34.641 153 90 5 3 149 9 157 1.37e-20 86.3
Msa1323140 AT1G70850.1 37.671 146 81 5 13 152 175 316 1.43e-21 89.0
Msa1323140 AT1G70850.1 34.641 153 90 5 3 149 9 157 1.37e-20 86.3
Msa1323140 AT1G70850.2 34.416 154 91 5 3 150 9 158 5.32e-21 85.9
Msa1323140 AT1G70870.1 45.833 96 50 2 56 150 44 138 1.31e-20 82.4
Msa1323140 AT4G23670.1 33.750 160 88 6 1 151 1 151 3.58e-20 81.6
Msa1323140 AT1G14930.1 29.560 159 104 5 1 155 1 155 6.45e-20 81.3
Msa1323140 AT2G01520.1 29.677 155 101 5 1 151 1 151 1.14e-18 77.8
Msa1323140 AT1G23120.1 29.078 141 94 4 10 149 10 145 3.35e-17 73.9
Msa1323140 AT2G01530.1 30.323 155 100 5 1 151 1 151 6.72e-17 73.2
Msa1323140 AT1G23120.2 29.078 141 94 4 10 149 60 195 9.42e-17 73.9
Msa1323140 AT4G14060.1 29.677 155 101 5 1 151 1 151 1.01e-16 72.8
Msa1323140 AT4G23680.1 29.221 154 101 5 1 150 1 150 1.61e-16 72.4
Msa1323140 AT1G70880.1 30.065 153 103 4 3 152 8 159 1.76e-16 72.4
Msa1323140 AT1G14940.1 28.302 159 106 5 1 155 1 155 1.93e-16 72.0
Msa1323140 AT1G24020.1 30.921 152 102 3 1 150 1 151 2.22e-16 72.0
Msa1323140 AT1G24020.2 30.921 152 102 3 1 150 1 151 2.22e-16 72.0
Msa1323140 AT1G23130.1 27.517 149 105 3 3 149 10 157 2.09e-15 69.7
Msa1323140 AT1G14950.1 27.044 159 108 5 1 155 1 155 6.91e-15 68.2
Msa1323140 AT1G30990.1 27.815 151 104 5 1 149 1 148 1.36e-14 67.4
Msa1323140 AT1G35260.1 29.932 147 92 5 9 149 8 149 1.65e-14 67.0
Msa1323140 AT3G26460.1 29.677 155 102 4 1 151 1 152 3.42e-14 66.2
Msa1323140 AT1G14960.1 25.161 155 112 4 1 153 1 153 2.29e-12 61.6
Msa1323140 AT3G26450.1 26.923 156 105 6 1 151 1 152 9.25e-12 59.7

Find 46 sgRNAs with CRISPR-Local

Find 250 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACACCATGTGTTTAAATA+AGG 0.277238 8_4:-31148075 Msa1323140:three_prime_UTR
GAAGACATTAATATTAAATA+AGG 0.331363 8_4:-31148135 Msa1323140:three_prime_UTR
AAGATGACCATCAATATCTT+TGG 0.335693 8_4:+31148190 None:intergenic
TTATGTGTGATGCAAATAAA+TGG 0.338325 8_4:-31147974 Msa1323140:three_prime_UTR
AAGATAACTTTCAAGCTGTT+TGG 0.342098 8_4:-31148368 Msa1323140:CDS
AAGAAATTAGAAACAAATAA+TGG 0.373905 8_4:+31150189 None:intergenic
ATGGAAGTTCTGCTGTTAGA+TGG 0.407851 8_4:-31148286 Msa1323140:CDS
TATCTTTGGTTCCTTTGCTC+AGG 0.433031 8_4:+31148204 None:intergenic
ATAAGGACGTCTGGTCCAAT+GGG 0.433127 8_4:-31148118 Msa1323140:three_prime_UTR
TGGAGATATTGAAAACTATA+AGG 0.439418 8_4:-31148345 Msa1323140:CDS
AGGTAGTCCATCCAGCCATT+TGG 0.459022 8_4:+31148224 None:intergenic
TAATATTAAATAAGGACGTC+TGG 0.466074 8_4:-31148127 Msa1323140:three_prime_UTR
AAAGGAACCAAAGATATTGA+TGG 0.479926 8_4:-31148197 Msa1323140:CDS
ATAACTTTCAAGCTGTTTGG+TGG 0.481618 8_4:-31148365 Msa1323140:CDS
GGTAAACTTATCACTGAACT+TGG 0.495374 8_4:-31150117 Msa1323140:CDS
AATAAGGACGTCTGGTCCAA+TGG 0.502748 8_4:-31148119 Msa1323140:three_prime_UTR
AGATTGGCATCACACTGATT+CGG 0.509801 8_4:-31149989 Msa1323140:CDS
AAAATATAGCGTTTCTCTAA+CGG 0.517199 8_4:-31147916 None:intergenic
TAACATAACAACAGATGGTA+AGG 0.518649 8_4:-31148438 Msa1323140:intron
AGCTTCATGAAGGAGAAGAT+TGG 0.526455 8_4:-31150005 Msa1323140:CDS
TAAACACTGGACTTATGTCA+TGG 0.531165 8_4:-31149965 Msa1323140:CDS
TGCACAAAAGCCAACCCCAT+TGG 0.544479 8_4:+31148103 None:intergenic
GACGTCTGGTCCAATGGGGT+TGG 0.549241 8_4:-31148113 Msa1323140:three_prime_UTR
TGTGTTTAAATAAGGACGTA+TGG 0.561712 8_4:-31148067 Msa1323140:three_prime_UTR
ATGTTGATACTCCAAATGGC+TGG 0.578150 8_4:-31148235 Msa1323140:CDS
GAAGTGCTTAACAAGGCTGA+TGG 0.580204 8_4:-31148305 Msa1323140:CDS
ATTGATGGTCATCTTGTCAA+GGG 0.586933 8_4:-31148182 Msa1323140:CDS
TGATACTCCAAATGGCTGGA+TGG 0.587093 8_4:-31148231 Msa1323140:CDS
TATTGATGGTCATCTTGTCA+AGG 0.589389 8_4:-31148183 Msa1323140:CDS
TACGTCCTTATTTAAACACA+TGG 0.590941 8_4:+31148070 None:intergenic
GAGGATGTTGATACTCCAAA+TGG 0.593330 8_4:-31148239 Msa1323140:CDS
AAACACTGGACTTATGTCAT+GGG 0.597721 8_4:-31149964 Msa1323140:intron
CATCATACAAAGCTTCATGA+AGG 0.600046 8_4:-31150015 Msa1323140:CDS
ACACTGATTCGGTTAAACAC+TGG 0.614381 8_4:-31149978 Msa1323140:CDS
GTAAACTTATCACTGAACTT+GGG 0.617151 8_4:-31150116 Msa1323140:CDS
AGGTACACACATGCAATGAG+AGG 0.626711 8_4:-31148418 Msa1323140:CDS
TGGATGGACTACCTGAGCAA+AGG 0.638104 8_4:-31148215 Msa1323140:CDS
CATCTTAGAAGTGCTTAACA+AGG 0.644958 8_4:-31148312 Msa1323140:CDS
TTGATGGTCATCTTGTCAAG+GGG 0.646397 8_4:-31148181 Msa1323140:CDS
ATGTTTGTATTCAATAAATG+TGG 0.646664 8_4:-31148014 Msa1323140:three_prime_UTR
GAACTTGGGATCAAAACACC+CGG 0.651017 8_4:-31150102 Msa1323140:CDS
AACAAACTATTTAAACACAT+AGG 0.669401 8_4:+31148043 None:intergenic
GGTACACACATGCAATGAGA+GGG 0.691358 8_4:-31148417 Msa1323140:CDS
TGATTATGAGAAGAACAATG+AGG 0.694185 8_4:-31148258 Msa1323140:CDS
TGATGGTCATCTTGTCAAGG+GGG 0.696400 8_4:-31148180 Msa1323140:CDS
TAAGGACGTCTGGTCCAATG+GGG 0.726492 8_4:-31148117 Msa1323140:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTATTAATAAACGTATTA+TGG + chr8_4:31148488-31148507 None:intergenic 10.0%
!!! AAAATGTTTTTTATTGTTTT+TGG - chr8_4:31149664-31149683 Msa1323140:intron 10.0%
!! ATTTATTAATAAACGTATTA+TGG + chr8_4:31148488-31148507 None:intergenic 10.0%
!!! AAAATGTTTTTTATTGTTTT+TGG - chr8_4:31149664-31149683 Msa1323140:intron 10.0%
!! AAGAAATTAGAAACAAATAA+TGG + chr8_4:31147952-31147971 None:intergenic 15.0%
!! ATTATAAATGAAGTCAATAT+AGG + chr8_4:31148403-31148422 None:intergenic 15.0%
!! AATGTTGAATATTTGAAAAT+GGG - chr8_4:31148535-31148554 Msa1323140:intron 15.0%
!! GGGTAAAATTAAATAAAAAA+AGG - chr8_4:31148555-31148574 Msa1323140:intron 15.0%
!!! TAAATTTTCACTTTTTGTTT+TGG + chr8_4:31149192-31149211 None:intergenic 15.0%
!!! TATAAAAGTCAAACTTTTAT+CGG - chr8_4:31149317-31149336 Msa1323140:intron 15.0%
!!! TAAGATAAAGTTTTTACTTT+TGG - chr8_4:31149579-31149598 Msa1323140:intron 15.0%
!! GAAGACATTAATATTAAATA+AGG - chr8_4:31150003-31150022 Msa1323140:CDS 15.0%
!! AAGAAATTAGAAACAAATAA+TGG + chr8_4:31147952-31147971 None:intergenic 15.0%
!! ATTATAAATGAAGTCAATAT+AGG + chr8_4:31148403-31148422 None:intergenic 15.0%
!! AATGTTGAATATTTGAAAAT+GGG - chr8_4:31148535-31148554 Msa1323140:intron 15.0%
!! GGGTAAAATTAAATAAAAAA+AGG - chr8_4:31148555-31148574 Msa1323140:intron 15.0%
!!! TAAATTTTCACTTTTTGTTT+TGG + chr8_4:31149192-31149211 None:intergenic 15.0%
!!! TATAAAAGTCAAACTTTTAT+CGG - chr8_4:31149317-31149336 Msa1323140:intron 15.0%
!!! TAAGATAAAGTTTTTACTTT+TGG - chr8_4:31149579-31149598 Msa1323140:intron 15.0%
!! GAAGACATTAATATTAAATA+AGG - chr8_4:31150003-31150022 Msa1323140:CDS 15.0%
!! TATAAGTTTCTTTCCAAAAA+TGG - chr8_4:31147990-31148009 Msa1323140:three_prime_UTR 20.0%
!! TTGATACTATCATAATAATC+TGG - chr8_4:31148290-31148309 Msa1323140:CDS 20.0%
!! GAATGTTGAATATTTGAAAA+TGG - chr8_4:31148534-31148553 Msa1323140:intron 20.0%
!! TATTACATACAACTCATATA+GGG + chr8_4:31148650-31148669 None:intergenic 20.0%
!! ATTTATCTTTCTAGATCTAA+TGG - chr8_4:31148706-31148725 Msa1323140:intron 20.0%
!!! TGCAATTTTATTTTAGTTCT+AGG + chr8_4:31148935-31148954 None:intergenic 20.0%
!!! TTATTGTATTTTCTCTCTTT+GGG + chr8_4:31149080-31149099 None:intergenic 20.0%
!!! ATTATTGTATTTTCTCTCTT+TGG + chr8_4:31149081-31149100 None:intergenic 20.0%
!! ATCAAAAATCAAATATCACA+AGG + chr8_4:31149288-31149307 None:intergenic 20.0%
!! ATCATAAAAACCTAATGATA+AGG + chr8_4:31149415-31149434 None:intergenic 20.0%
!!! TTAGGTTTTTATGATTTCTT+TGG - chr8_4:31149420-31149439 Msa1323140:intron 20.0%
!!! TGGTGAAACTAATATTTTTA+AGG - chr8_4:31149440-31149459 Msa1323140:intron 20.0%
!! AACAAACTATTTAAACACAT+AGG + chr8_4:31150098-31150117 None:intergenic 20.0%
!! ATGTTTGTATTCAATAAATG+TGG - chr8_4:31150124-31150143 Msa1323140:CDS 20.0%
!! TATAAGTTTCTTTCCAAAAA+TGG - chr8_4:31147990-31148009 Msa1323140:three_prime_UTR 20.0%
!! TTGATACTATCATAATAATC+TGG - chr8_4:31148290-31148309 Msa1323140:CDS 20.0%
!! GAATGTTGAATATTTGAAAA+TGG - chr8_4:31148534-31148553 Msa1323140:intron 20.0%
!! TATTACATACAACTCATATA+GGG + chr8_4:31148650-31148669 None:intergenic 20.0%
!! ATTTATCTTTCTAGATCTAA+TGG - chr8_4:31148706-31148725 Msa1323140:intron 20.0%
!!! TGCAATTTTATTTTAGTTCT+AGG + chr8_4:31148935-31148954 None:intergenic 20.0%
!!! TTATTGTATTTTCTCTCTTT+GGG + chr8_4:31149080-31149099 None:intergenic 20.0%
!!! ATTATTGTATTTTCTCTCTT+TGG + chr8_4:31149081-31149100 None:intergenic 20.0%
!! ATCAAAAATCAAATATCACA+AGG + chr8_4:31149288-31149307 None:intergenic 20.0%
!! ATCATAAAAACCTAATGATA+AGG + chr8_4:31149415-31149434 None:intergenic 20.0%
!!! TTAGGTTTTTATGATTTCTT+TGG - chr8_4:31149420-31149439 Msa1323140:intron 20.0%
!!! TGGTGAAACTAATATTTTTA+AGG - chr8_4:31149440-31149459 Msa1323140:intron 20.0%
!! AACAAACTATTTAAACACAT+AGG + chr8_4:31150098-31150117 None:intergenic 20.0%
!! ATGTTTGTATTCAATAAATG+TGG - chr8_4:31150124-31150143 Msa1323140:CDS 20.0%
! ATTGCCTTTATAAGTTCTTT+AGG + chr8_4:31148317-31148336 None:intergenic 25.0%
! ATTACATACAACTCATATAG+GGG + chr8_4:31148649-31148668 None:intergenic 25.0%
! CTATTACATACAACTCATAT+AGG + chr8_4:31148651-31148670 None:intergenic 25.0%
!!! AATTTTTCGATCTAGTGTAA+TGG - chr8_4:31148814-31148833 Msa1323140:intron 25.0%
! ATAAAATTGCAGAATGATAG+AGG - chr8_4:31148944-31148963 Msa1323140:intron 25.0%
! TAAAATTGCAGAATGATAGA+GGG - chr8_4:31148945-31148964 Msa1323140:intron 25.0%
! ATAGAGGGAAATTATTGTTA+AGG - chr8_4:31148960-31148979 Msa1323140:intron 25.0%
! GCTACAAAATTAATCTCTTA+GGG - chr8_4:31148982-31149001 Msa1323140:intron 25.0%
!!! ATTTTACTAATGAAGAGTAC+AGG - chr8_4:31149041-31149060 Msa1323140:intron 25.0%
! CAAATCAAATTGTGTTCAAA+TGG + chr8_4:31149133-31149152 None:intergenic 25.0%
!!! CTGTTTAGGTTATTTTGATT+AGG + chr8_4:31149163-31149182 None:intergenic 25.0%
!!! ATTTTCACTTTTTGTTTTGG+TGG + chr8_4:31149189-31149208 None:intergenic 25.0%
!!! TATTTGATTTTTGATCCTTG+TGG - chr8_4:31149293-31149312 Msa1323140:intron 25.0%
! AATGCTAACAAATGTTCTTA+AGG + chr8_4:31149619-31149638 None:intergenic 25.0%
! TGGAGATATTGAAAACTATA+AGG - chr8_4:31149793-31149812 Msa1323140:intron 25.0%
! TAATATTAAATAAGGACGTC+TGG - chr8_4:31150011-31150030 Msa1323140:CDS 25.0%
! TTACACCATGTGTTTAAATA+AGG - chr8_4:31150063-31150082 Msa1323140:CDS 25.0%
! TTATGTGTGATGCAAATAAA+TGG - chr8_4:31150164-31150183 Msa1323140:five_prime_UTR 25.0%
! ATTGCCTTTATAAGTTCTTT+AGG + chr8_4:31148317-31148336 None:intergenic 25.0%
! ATTACATACAACTCATATAG+GGG + chr8_4:31148649-31148668 None:intergenic 25.0%
! CTATTACATACAACTCATAT+AGG + chr8_4:31148651-31148670 None:intergenic 25.0%
!!! AATTTTTCGATCTAGTGTAA+TGG - chr8_4:31148814-31148833 Msa1323140:intron 25.0%
! ATAAAATTGCAGAATGATAG+AGG - chr8_4:31148944-31148963 Msa1323140:intron 25.0%
! TAAAATTGCAGAATGATAGA+GGG - chr8_4:31148945-31148964 Msa1323140:intron 25.0%
! ATAGAGGGAAATTATTGTTA+AGG - chr8_4:31148960-31148979 Msa1323140:intron 25.0%
! GCTACAAAATTAATCTCTTA+GGG - chr8_4:31148982-31149001 Msa1323140:intron 25.0%
!!! ATTTTACTAATGAAGAGTAC+AGG - chr8_4:31149041-31149060 Msa1323140:intron 25.0%
! CAAATCAAATTGTGTTCAAA+TGG + chr8_4:31149133-31149152 None:intergenic 25.0%
!!! CTGTTTAGGTTATTTTGATT+AGG + chr8_4:31149163-31149182 None:intergenic 25.0%
!!! ATTTTCACTTTTTGTTTTGG+TGG + chr8_4:31149189-31149208 None:intergenic 25.0%
!!! TATTTGATTTTTGATCCTTG+TGG - chr8_4:31149293-31149312 Msa1323140:intron 25.0%
! AATGCTAACAAATGTTCTTA+AGG + chr8_4:31149619-31149638 None:intergenic 25.0%
! TGGAGATATTGAAAACTATA+AGG - chr8_4:31149793-31149812 Msa1323140:intron 25.0%
! TAATATTAAATAAGGACGTC+TGG - chr8_4:31150011-31150030 Msa1323140:CDS 25.0%
! TTACACCATGTGTTTAAATA+AGG - chr8_4:31150063-31150082 Msa1323140:CDS 25.0%
! TTATGTGTGATGCAAATAAA+TGG - chr8_4:31150164-31150183 Msa1323140:five_prime_UTR 25.0%
GTAAACTTATCACTGAACTT+GGG - chr8_4:31148022-31148041 Msa1323140:three_prime_UTR 30.0%
! TAGTTTGAAGAACTTTTCAC+CGG + chr8_4:31148058-31148077 None:intergenic 30.0%
AATCAAACAAGATGATGACA+TGG + chr8_4:31148231-31148250 None:intergenic 30.0%
TGGACCTAAAGAACTTATAA+AGG - chr8_4:31148310-31148329 Msa1323140:CDS 30.0%
CATTGTATGGATTTATGCTT+AGG - chr8_4:31148614-31148633 Msa1323140:intron 30.0%
!! GTTGTATGTAATAGTTCTAG+TGG - chr8_4:31148657-31148676 Msa1323140:intron 30.0%
! GTAATGGAGAAGTAATTACA+TGG - chr8_4:31148830-31148849 Msa1323140:intron 30.0%
TAATTACATGGCACATATCT+AGG - chr8_4:31148842-31148861 Msa1323140:intron 30.0%
! TGATTTTAAACTATCCTGTG+AGG - chr8_4:31148894-31148913 Msa1323140:intron 30.0%
GGCTACAAAATTAATCTCTT+AGG - chr8_4:31148981-31149000 Msa1323140:intron 30.0%
CTTAGGGTTAATAAAACGTA+TGG - chr8_4:31148998-31149017 Msa1323140:intron 30.0%
ACAATATCTCTTGGAAGTTA+CGG + chr8_4:31149240-31149259 None:intergenic 30.0%
!! GTTTGACTTTTATAACCACA+AGG + chr8_4:31149311-31149330 None:intergenic 30.0%
TAACATAACAACAGATGGTA+AGG - chr8_4:31149700-31149719 Msa1323140:intron 30.0%
AAGATAACTTTCAAGCTGTT+TGG - chr8_4:31149770-31149789 Msa1323140:intron 30.0%
TGATTATGAGAAGAACAATG+AGG - chr8_4:31149880-31149899 Msa1323140:intron 30.0%
AAAGGAACCAAAGATATTGA+TGG - chr8_4:31149941-31149960 Msa1323140:intron 30.0%
AAGATGACCATCAATATCTT+TGG + chr8_4:31149951-31149970 None:intergenic 30.0%
! TACGTCCTTATTTAAACACA+TGG + chr8_4:31150071-31150090 None:intergenic 30.0%
TGTGTTTAAATAAGGACGTA+TGG - chr8_4:31150071-31150090 Msa1323140:CDS 30.0%
GTAAACTTATCACTGAACTT+GGG - chr8_4:31148022-31148041 Msa1323140:three_prime_UTR 30.0%
! TAGTTTGAAGAACTTTTCAC+CGG + chr8_4:31148058-31148077 None:intergenic 30.0%
AATCAAACAAGATGATGACA+TGG + chr8_4:31148231-31148250 None:intergenic 30.0%
TGGACCTAAAGAACTTATAA+AGG - chr8_4:31148310-31148329 Msa1323140:CDS 30.0%
CATTGTATGGATTTATGCTT+AGG - chr8_4:31148614-31148633 Msa1323140:intron 30.0%
!! GTTGTATGTAATAGTTCTAG+TGG - chr8_4:31148657-31148676 Msa1323140:intron 30.0%
! GTAATGGAGAAGTAATTACA+TGG - chr8_4:31148830-31148849 Msa1323140:intron 30.0%
TAATTACATGGCACATATCT+AGG - chr8_4:31148842-31148861 Msa1323140:intron 30.0%
! TGATTTTAAACTATCCTGTG+AGG - chr8_4:31148894-31148913 Msa1323140:intron 30.0%
GGCTACAAAATTAATCTCTT+AGG - chr8_4:31148981-31149000 Msa1323140:intron 30.0%
CTTAGGGTTAATAAAACGTA+TGG - chr8_4:31148998-31149017 Msa1323140:intron 30.0%
ACAATATCTCTTGGAAGTTA+CGG + chr8_4:31149240-31149259 None:intergenic 30.0%
!! GTTTGACTTTTATAACCACA+AGG + chr8_4:31149311-31149330 None:intergenic 30.0%
TAACATAACAACAGATGGTA+AGG - chr8_4:31149700-31149719 Msa1323140:intron 30.0%
AAGATAACTTTCAAGCTGTT+TGG - chr8_4:31149770-31149789 Msa1323140:intron 30.0%
TGATTATGAGAAGAACAATG+AGG - chr8_4:31149880-31149899 Msa1323140:intron 30.0%
AAAGGAACCAAAGATATTGA+TGG - chr8_4:31149941-31149960 Msa1323140:intron 30.0%
AAGATGACCATCAATATCTT+TGG + chr8_4:31149951-31149970 None:intergenic 30.0%
! TACGTCCTTATTTAAACACA+TGG + chr8_4:31150071-31150090 None:intergenic 30.0%
TGTGTTTAAATAAGGACGTA+TGG - chr8_4:31150071-31150090 Msa1323140:CDS 30.0%
GGTAAACTTATCACTGAACT+TGG - chr8_4:31148021-31148040 Msa1323140:three_prime_UTR 35.0%
! AGTTTGAAGAACTTTTCACC+GGG + chr8_4:31148057-31148076 None:intergenic 35.0%
CATCATACAAAGCTTCATGA+AGG - chr8_4:31148123-31148142 Msa1323140:three_prime_UTR 35.0%
TAAACACTGGACTTATGTCA+TGG - chr8_4:31148173-31148192 Msa1323140:CDS 35.0%
AAACACTGGACTTATGTCAT+GGG - chr8_4:31148174-31148193 Msa1323140:CDS 35.0%
!! TGCTACTTTAGTGCATTGTA+TGG - chr8_4:31148601-31148620 Msa1323140:intron 35.0%
! AATTCAAGTTCGAGTTCAAC+TGG - chr8_4:31148765-31148784 Msa1323140:intron 35.0%
TGGCACATATCTAGGTAAAA+CGG - chr8_4:31148850-31148869 Msa1323140:intron 35.0%
GATTAACTTTGTAACCTCAC+AGG + chr8_4:31148911-31148930 None:intergenic 35.0%
TGTGTTCAAATGGAACTTTG+TGG + chr8_4:31149123-31149142 None:intergenic 35.0%
! GTTTGCTACGATAGCAAAAA+GGG + chr8_4:31149218-31149237 None:intergenic 35.0%
CCATAAAGCACAATATCTCT+TGG + chr8_4:31149249-31149268 None:intergenic 35.0%
CCAAGAGATATTGTGCTTTA+TGG - chr8_4:31149246-31149265 Msa1323140:intron 35.0%
! GCATCGATATCCTTATCATT+AGG - chr8_4:31149402-31149421 Msa1323140:intron 35.0%
ATCCTTAACTAGTGCTCTAA+GGG + chr8_4:31149481-31149500 None:intergenic 35.0%
AATCCTTAACTAGTGCTCTA+AGG + chr8_4:31149482-31149501 None:intergenic 35.0%
ACCATCTGTTGTTATGTTAG+AGG + chr8_4:31149699-31149718 None:intergenic 35.0%
ATAACTTTCAAGCTGTTTGG+TGG - chr8_4:31149773-31149792 Msa1323140:intron 35.0%
!! CATCTTAGAAGTGCTTAACA+AGG - chr8_4:31149826-31149845 Msa1323140:intron 35.0%
! TATTGATGGTCATCTTGTCA+AGG - chr8_4:31149955-31149974 Msa1323140:intron 35.0%
! ATTGATGGTCATCTTGTCAA+GGG - chr8_4:31149956-31149975 Msa1323140:intron 35.0%
GGTAAACTTATCACTGAACT+TGG - chr8_4:31148021-31148040 Msa1323140:three_prime_UTR 35.0%
! AGTTTGAAGAACTTTTCACC+GGG + chr8_4:31148057-31148076 None:intergenic 35.0%
CATCATACAAAGCTTCATGA+AGG - chr8_4:31148123-31148142 Msa1323140:three_prime_UTR 35.0%
TAAACACTGGACTTATGTCA+TGG - chr8_4:31148173-31148192 Msa1323140:CDS 35.0%
AAACACTGGACTTATGTCAT+GGG - chr8_4:31148174-31148193 Msa1323140:CDS 35.0%
!! TGCTACTTTAGTGCATTGTA+TGG - chr8_4:31148601-31148620 Msa1323140:intron 35.0%
! AATTCAAGTTCGAGTTCAAC+TGG - chr8_4:31148765-31148784 Msa1323140:intron 35.0%
TGGCACATATCTAGGTAAAA+CGG - chr8_4:31148850-31148869 Msa1323140:intron 35.0%
GATTAACTTTGTAACCTCAC+AGG + chr8_4:31148911-31148930 None:intergenic 35.0%
TGTGTTCAAATGGAACTTTG+TGG + chr8_4:31149123-31149142 None:intergenic 35.0%
! GTTTGCTACGATAGCAAAAA+GGG + chr8_4:31149218-31149237 None:intergenic 35.0%
CCATAAAGCACAATATCTCT+TGG + chr8_4:31149249-31149268 None:intergenic 35.0%
CCAAGAGATATTGTGCTTTA+TGG - chr8_4:31149246-31149265 Msa1323140:intron 35.0%
! GCATCGATATCCTTATCATT+AGG - chr8_4:31149402-31149421 Msa1323140:intron 35.0%
ATCCTTAACTAGTGCTCTAA+GGG + chr8_4:31149481-31149500 None:intergenic 35.0%
AATCCTTAACTAGTGCTCTA+AGG + chr8_4:31149482-31149501 None:intergenic 35.0%
ACCATCTGTTGTTATGTTAG+AGG + chr8_4:31149699-31149718 None:intergenic 35.0%
ATAACTTTCAAGCTGTTTGG+TGG - chr8_4:31149773-31149792 Msa1323140:intron 35.0%
!! CATCTTAGAAGTGCTTAACA+AGG - chr8_4:31149826-31149845 Msa1323140:intron 35.0%
! TATTGATGGTCATCTTGTCA+AGG - chr8_4:31149955-31149974 Msa1323140:intron 35.0%
! ATTGATGGTCATCTTGTCAA+GGG - chr8_4:31149956-31149975 Msa1323140:intron 35.0%
TTTCCAAAAATGGTACTCGC+AGG - chr8_4:31148000-31148019 Msa1323140:three_prime_UTR 40.0%
! TTACCTGCGAGTACCATTTT+TGG + chr8_4:31148006-31148025 None:intergenic 40.0%
AGCTTCATGAAGGAGAAGAT+TGG - chr8_4:31148133-31148152 Msa1323140:three_prime_UTR 40.0%
! AGATTGGCATCACACTGATT+CGG - chr8_4:31148149-31148168 Msa1323140:exon 40.0%
!! ACACTGATTCGGTTAAACAC+TGG - chr8_4:31148160-31148179 Msa1323140:exon 40.0%
CTATACCAAGCAACTCATGT+GGG - chr8_4:31148741-31148760 Msa1323140:intron 40.0%
TATACCAAGCAACTCATGTG+GGG - chr8_4:31148742-31148761 Msa1323140:intron 40.0%
ATGGAACTTTGTGGTCGTAA+TGG + chr8_4:31149114-31149133 None:intergenic 40.0%
!!! TGTTTTGGTGGCAACTGTTT+AGG + chr8_4:31149177-31149196 None:intergenic 40.0%
!! GGTTTGCTACGATAGCAAAA+AGG + chr8_4:31149219-31149238 None:intergenic 40.0%
! TTCCCTTAGAGCACTAGTTA+AGG - chr8_4:31149476-31149495 Msa1323140:intron 40.0%
GCCTCTAACATAACAACAGA+TGG - chr8_4:31149695-31149714 Msa1323140:intron 40.0%
ATGGAAGTTCTGCTGTTAGA+TGG - chr8_4:31149852-31149871 Msa1323140:intron 40.0%
GAGGATGTTGATACTCCAAA+TGG - chr8_4:31149899-31149918 Msa1323140:intron 40.0%
ATGTTGATACTCCAAATGGC+TGG - chr8_4:31149903-31149922 Msa1323140:intron 40.0%
! TATCTTTGGTTCCTTTGCTC+AGG + chr8_4:31149937-31149956 None:intergenic 40.0%
! TTGATGGTCATCTTGTCAAG+GGG - chr8_4:31149957-31149976 Msa1323140:intron 40.0%
TTTCCAAAAATGGTACTCGC+AGG - chr8_4:31148000-31148019 Msa1323140:three_prime_UTR 40.0%
! TTACCTGCGAGTACCATTTT+TGG + chr8_4:31148006-31148025 None:intergenic 40.0%
AGCTTCATGAAGGAGAAGAT+TGG - chr8_4:31148133-31148152 Msa1323140:three_prime_UTR 40.0%
! AGATTGGCATCACACTGATT+CGG - chr8_4:31148149-31148168 Msa1323140:exon 40.0%
!! ACACTGATTCGGTTAAACAC+TGG - chr8_4:31148160-31148179 Msa1323140:exon 40.0%
CTATACCAAGCAACTCATGT+GGG - chr8_4:31148741-31148760 Msa1323140:intron 40.0%
TATACCAAGCAACTCATGTG+GGG - chr8_4:31148742-31148761 Msa1323140:intron 40.0%
ATGGAACTTTGTGGTCGTAA+TGG + chr8_4:31149114-31149133 None:intergenic 40.0%
!!! TGTTTTGGTGGCAACTGTTT+AGG + chr8_4:31149177-31149196 None:intergenic 40.0%
!! GGTTTGCTACGATAGCAAAA+AGG + chr8_4:31149219-31149238 None:intergenic 40.0%
! TTCCCTTAGAGCACTAGTTA+AGG - chr8_4:31149476-31149495 Msa1323140:intron 40.0%
GCCTCTAACATAACAACAGA+TGG - chr8_4:31149695-31149714 Msa1323140:intron 40.0%
ATGGAAGTTCTGCTGTTAGA+TGG - chr8_4:31149852-31149871 Msa1323140:intron 40.0%
GAGGATGTTGATACTCCAAA+TGG - chr8_4:31149899-31149918 Msa1323140:intron 40.0%
ATGTTGATACTCCAAATGGC+TGG - chr8_4:31149903-31149922 Msa1323140:intron 40.0%
! TATCTTTGGTTCCTTTGCTC+AGG + chr8_4:31149937-31149956 None:intergenic 40.0%
! TTGATGGTCATCTTGTCAAG+GGG - chr8_4:31149957-31149976 Msa1323140:intron 40.0%
GAACTTGGGATCAAAACACC+CGG - chr8_4:31148036-31148055 Msa1323140:three_prime_UTR 45.0%
TTGCTTGGTATAGCAGGTGT+GGG + chr8_4:31148734-31148753 None:intergenic 45.0%
CATGAGTTGCTTGGTATAGC+AGG + chr8_4:31148740-31148759 None:intergenic 45.0%
GCTATACCAAGCAACTCATG+TGG - chr8_4:31148740-31148759 Msa1323140:intron 45.0%
AATTCCCCACATGAGTTGCT+TGG + chr8_4:31148749-31148768 None:intergenic 45.0%
! CAAGTTCGAGTTCAACTGGA+TGG - chr8_4:31148769-31148788 Msa1323140:intron 45.0%
GAGTTCAACTGGATGGTTGA+GGG - chr8_4:31148776-31148795 Msa1323140:intron 45.0%
AGTTCAACTGGATGGTTGAG+GGG - chr8_4:31148777-31148796 Msa1323140:intron 45.0%
AGGTACACACATGCAATGAG+AGG - chr8_4:31149720-31149739 Msa1323140:intron 45.0%
GGTACACACATGCAATGAGA+GGG - chr8_4:31149721-31149740 Msa1323140:intron 45.0%
! GAAGTGCTTAACAAGGCTGA+TGG - chr8_4:31149833-31149852 Msa1323140:intron 45.0%
TGATACTCCAAATGGCTGGA+TGG - chr8_4:31149907-31149926 Msa1323140:intron 45.0%
TGATGGTCATCTTGTCAAGG+GGG - chr8_4:31149958-31149977 Msa1323140:intron 45.0%
TCATCTTGTCAAGGGGGAAA+AGG - chr8_4:31149964-31149983 Msa1323140:intron 45.0%
AATAAGGACGTCTGGTCCAA+TGG - chr8_4:31150019-31150038 Msa1323140:CDS 45.0%
ATAAGGACGTCTGGTCCAAT+GGG - chr8_4:31150020-31150039 Msa1323140:CDS 45.0%
GAACTTGGGATCAAAACACC+CGG - chr8_4:31148036-31148055 Msa1323140:three_prime_UTR 45.0%
TTGCTTGGTATAGCAGGTGT+GGG + chr8_4:31148734-31148753 None:intergenic 45.0%
CATGAGTTGCTTGGTATAGC+AGG + chr8_4:31148740-31148759 None:intergenic 45.0%
GCTATACCAAGCAACTCATG+TGG - chr8_4:31148740-31148759 Msa1323140:intron 45.0%
AATTCCCCACATGAGTTGCT+TGG + chr8_4:31148749-31148768 None:intergenic 45.0%
! CAAGTTCGAGTTCAACTGGA+TGG - chr8_4:31148769-31148788 Msa1323140:intron 45.0%
GAGTTCAACTGGATGGTTGA+GGG - chr8_4:31148776-31148795 Msa1323140:intron 45.0%
AGTTCAACTGGATGGTTGAG+GGG - chr8_4:31148777-31148796 Msa1323140:intron 45.0%
AGGTACACACATGCAATGAG+AGG - chr8_4:31149720-31149739 Msa1323140:intron 45.0%
GGTACACACATGCAATGAGA+GGG - chr8_4:31149721-31149740 Msa1323140:intron 45.0%
! GAAGTGCTTAACAAGGCTGA+TGG - chr8_4:31149833-31149852 Msa1323140:intron 45.0%
TGATACTCCAAATGGCTGGA+TGG - chr8_4:31149907-31149926 Msa1323140:intron 45.0%
TGATGGTCATCTTGTCAAGG+GGG - chr8_4:31149958-31149977 Msa1323140:intron 45.0%
TCATCTTGTCAAGGGGGAAA+AGG - chr8_4:31149964-31149983 Msa1323140:intron 45.0%
AATAAGGACGTCTGGTCCAA+TGG - chr8_4:31150019-31150038 Msa1323140:CDS 45.0%
ATAAGGACGTCTGGTCCAAT+GGG - chr8_4:31150020-31150039 Msa1323140:CDS 45.0%
!! TTAAAGTAATATTAAAAATA+AGG + chr8_4:31149643-31149662 None:intergenic 5.0%
!! TTAAAGTAATATTAAAAATA+AGG + chr8_4:31149643-31149662 None:intergenic 5.0%
GTTGCTTGGTATAGCAGGTG+TGG + chr8_4:31148735-31148754 None:intergenic 50.0%
CGAGTTCAACTGGATGGTTG+AGG - chr8_4:31148775-31148794 Msa1323140:intron 50.0%
! GTTGAGGGGTAGTCTTGACT+TGG - chr8_4:31148791-31148810 Msa1323140:intron 50.0%
AGGTAGTCCATCCAGCCATT+TGG + chr8_4:31149917-31149936 None:intergenic 50.0%
!! TGGATGGACTACCTGAGCAA+AGG - chr8_4:31149923-31149942 Msa1323140:intron 50.0%
TAAGGACGTCTGGTCCAATG+GGG - chr8_4:31150021-31150040 Msa1323140:CDS 50.0%
TGCACAAAAGCCAACCCCAT+TGG + chr8_4:31150038-31150057 None:intergenic 50.0%
GTTGCTTGGTATAGCAGGTG+TGG + chr8_4:31148735-31148754 None:intergenic 50.0%
CGAGTTCAACTGGATGGTTG+AGG - chr8_4:31148775-31148794 Msa1323140:intron 50.0%
! GTTGAGGGGTAGTCTTGACT+TGG - chr8_4:31148791-31148810 Msa1323140:intron 50.0%
AGGTAGTCCATCCAGCCATT+TGG + chr8_4:31149917-31149936 None:intergenic 50.0%
!! TGGATGGACTACCTGAGCAA+AGG - chr8_4:31149923-31149942 Msa1323140:intron 50.0%
TAAGGACGTCTGGTCCAATG+GGG - chr8_4:31150021-31150040 Msa1323140:CDS 50.0%
TGCACAAAAGCCAACCCCAT+TGG + chr8_4:31150038-31150057 None:intergenic 50.0%
GACGTCTGGTCCAATGGGGT+TGG - chr8_4:31150025-31150044 Msa1323140:CDS 60.0%
GACGTCTGGTCCAATGGGGT+TGG - chr8_4:31150025-31150044 Msa1323140:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 31147919 31150241 31147919 ID=Msa1323140;Name=Msa1323140
chr8_4 mRNA 31147919 31150241 31147919 ID=Msa1323140-mRNA-1;Parent=Msa1323140;Name=Msa1323140-mRNA-1;_AED=0.03;_eAED=0.03;_QI=90|1|1|1|1|1|2|244|156
chr8_4 exon 31147919 31148446 31147919 ID=Msa1323140-mRNA-1:exon:10180;Parent=Msa1323140-mRNA-1
chr8_4 exon 31149965 31150241 31149965 ID=Msa1323140-mRNA-1:exon:10179;Parent=Msa1323140-mRNA-1
chr8_4 five_prime_UTR 31150152 31150241 31150152 ID=Msa1323140-mRNA-1:five_prime_utr;Parent=Msa1323140-mRNA-1
chr8_4 CDS 31149965 31150151 31149965 ID=Msa1323140-mRNA-1:cds;Parent=Msa1323140-mRNA-1
chr8_4 CDS 31148163 31148446 31148163 ID=Msa1323140-mRNA-1:cds;Parent=Msa1323140-mRNA-1
chr8_4 three_prime_UTR 31147919 31148162 31147919 ID=Msa1323140-mRNA-1:three_prime_utr;Parent=Msa1323140-mRNA-1
Gene Sequence

>Msa1323140

ATGGTACTCGCAGGTAAACTTATCACTGAACTTGGGATCAAAACACCCGGTGAAAAGTTCTTCAAACTATTTGCAACTCAACTTCACGAAGTGCAAAACGTTGTTGAAAGATGTCATCATACAAAGCTTCATGAAGGAGAAGATTGGCATCACACTGATTCGGTTAAACACTGGACTTATGTCATGGATGGTAAGGTACACACATGCAATGAGAGGGTTGAAGAAGTTGATGAGCAAAACAAAAAGATAACTTTCAAGCTGTTTGGTGGAGATATTGAAAACTATAAGGTCTTTAAGCTCATCTTAGAAGTGCTTAACAAGGCTGATGGAAGTTCTGCTGTTAGATGGACTATTGATTATGAGAAGAACAATGAGGATGTTGATACTCCAAATGGCTGGATGGACTACCTGAGCAAAGGAACCAAAGATATTGATGGTCATCTTGTCAAGGGGGAAAAGGTAGCTCTATAA
Protein sequence

>Msa1323140

MVLAGKLITELGIKTPGEKFFKLFATQLHEVQNVVERCHHTKLHEGEDWHHTDSVKHWTYVMDGKVHTCNERVEEVDEQNKKITFKLFGGDIENYKVFKLILEVLNKADGSSAVRWTIDYEKNNEDVDTPNGWMDYLSKGTKDIDGHLVKGEKVAL*