Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1323350 | MCH99121.1 | 96.639 | 119 | 4 | 0 | 3 | 121 | 20 | 138 | 5.83e-78 | 236 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1323350 | sp|P45432|CSN1_ARATH | 81.513 | 119 | 22 | 0 | 3 | 121 | 323 | 441 | 1.61e-65 | 207 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1323350 | A0A392NI33 | 96.639 | 119 | 4 | 0 | 3 | 121 | 20 | 138 | 2.78e-78 | 236 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1283800 | Msa1323350 | 0.815519 | 8.869702e-52 | -8.615850e-47 |
| Msa0478920 | Msa1323350 | 0.806004 | 1.015054e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1323350 | MtrunA17_Chr8g0350361 | 98.347 | 121 | 2 | 0 | 1 | 121 | 325 | 445 | 6.03e-82 | 246 |
| Msa1323350 | MtrunA17_Chr7g0246691 | 75.630 | 119 | 29 | 0 | 3 | 121 | 325 | 443 | 2.67e-59 | 188 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1323350 | AT3G61140.1 | 81.513 | 119 | 22 | 0 | 3 | 121 | 323 | 441 | 1.64e-66 | 207 |
Find 22 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAAACATGACTTCAATATT+AGG | 0.175164 | 8_4:-31658832 | Msa1323350:CDS |
| TAAACATGACTTCAATATTA+GGG | 0.299578 | 8_4:-31658831 | Msa1323350:CDS |
| TTTCAAAGGGTTCTAGAAAC+TGG | 0.314327 | 8_4:-31658905 | Msa1323350:CDS |
| ATGGGAGTGTATACTGGATA+AGG | 0.390701 | 8_4:+31659329 | None:intergenic |
| ATACAAATGGGAGTGTATAC+TGG | 0.417410 | 8_4:+31659323 | None:intergenic |
| GCATTCAAGACAACTGTTGT+TGG | 0.425392 | 8_4:-31659274 | Msa1323350:CDS |
| TTCAAATCAACAGATACAAA+TGG | 0.429576 | 8_4:+31659310 | None:intergenic |
| ATCTGTTGATTTGAATATGA+TGG | 0.461634 | 8_4:-31659302 | Msa1323350:CDS |
| GATTACTGATAATCAAATAC+AGG | 0.501737 | 8_4:-31659227 | Msa1323350:intron |
| TCAAATCAACAGATACAAAT+GGG | 0.503406 | 8_4:+31659311 | None:intergenic |
| TGGGAGTGTATACTGGATAA+GGG | 0.513868 | 8_4:+31659330 | None:intergenic |
| AAGGAATGCCACCTTTCAAA+GGG | 0.513987 | 8_4:-31658918 | Msa1323350:CDS |
| AAAGGAATGCCACCTTTCAA+AGG | 0.518395 | 8_4:-31658919 | Msa1323350:CDS |
| CCTGAAATCAAATATGTCGC+TGG | 0.518919 | 8_4:-31659407 | None:intergenic |
| TTCTAGAACCCTTTGAAAGG+TGG | 0.540033 | 8_4:+31658910 | None:intergenic |
| AAAGGGTTCTAGAAACTGGA+AGG | 0.545587 | 8_4:-31658901 | Msa1323350:CDS |
| ACTTCAATATTAGGGCATCA+AGG | 0.566142 | 8_4:-31658823 | Msa1323350:CDS |
| AGTTTCTAGAACCCTTTGAA+AGG | 0.589137 | 8_4:+31658907 | None:intergenic |
| ATGCACAACATGCAGATCAA+AGG | 0.590335 | 8_4:-31658937 | Msa1323350:CDS |
| AAGATTTGATCTCAGCAACA+TGG | 0.606774 | 8_4:+31658856 | None:intergenic |
| AAGGGTTCTAGAAACTGGAA+GGG | 0.656638 | 8_4:-31658900 | Msa1323350:CDS |
| GATCGTAAAGTGTCTCAACA+TGG | 0.668297 | 8_4:+31659368 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCCAAAAGTTTCATAAATT+AGG | + | chr8_4:31659212-31659231 | None:intergenic | 20.0% |
| !! | TTAAACATGACTTCAATATT+AGG | - | chr8_4:31659376-31659395 | Msa1323350:CDS | 20.0% |
| !! | TAAACATGACTTCAATATTA+GGG | - | chr8_4:31659377-31659396 | Msa1323350:CDS | 20.0% |
| ! | TCAAATCAACAGATACAAAT+GGG | + | chr8_4:31658900-31658919 | None:intergenic | 25.0% |
| ! | TTCAAATCAACAGATACAAA+TGG | + | chr8_4:31658901-31658920 | None:intergenic | 25.0% |
| !!! | ATCTGTTGATTTGAATATGA+TGG | - | chr8_4:31658906-31658925 | Msa1323350:CDS | 25.0% |
| ! | GATTACTGATAATCAAATAC+AGG | - | chr8_4:31658981-31659000 | Msa1323350:intron | 25.0% |
| ! | CAATTTATAGTAACTCAAAC+CGG | + | chr8_4:31659008-31659027 | None:intergenic | 25.0% |
| !! | TAATTTTCTATAACATAGCC+TGG | + | chr8_4:31659053-31659072 | None:intergenic | 25.0% |
| !! | GTCTCTTTTACTATAAAAGA+AGG | - | chr8_4:31659156-31659175 | Msa1323350:intron | 25.0% |
| !! | CTCCTAATTTATGAAACTTT+TGG | - | chr8_4:31659207-31659226 | Msa1323350:intron | 25.0% |
| ! | ATACAAATGGGAGTGTATAC+TGG | + | chr8_4:31658888-31658907 | None:intergenic | 35.0% |
| CTGATAATCAAATACAGGTC+CGG | - | chr8_4:31658986-31659005 | Msa1323350:intron | 35.0% | |
| TTGTGCTTACAAATTCAACC+AGG | - | chr8_4:31659032-31659051 | Msa1323350:intron | 35.0% | |
| AATTGCATGCCGAAGTTATT+TGG | + | chr8_4:31659122-31659141 | None:intergenic | 35.0% | |
| AGAAGGAAAAAAAGCCTGTT+TGG | - | chr8_4:31659173-31659192 | Msa1323350:intron | 35.0% | |
| GAAGGAAAAAAAGCCTGTTT+GGG | - | chr8_4:31659174-31659193 | Msa1323350:intron | 35.0% | |
| !! | AGTTTCTAGAACCCTTTGAA+AGG | + | chr8_4:31659304-31659323 | None:intergenic | 35.0% |
| !! | TTTCAAAGGGTTCTAGAAAC+TGG | - | chr8_4:31659303-31659322 | Msa1323350:CDS | 35.0% |
| AAGATTTGATCTCAGCAACA+TGG | + | chr8_4:31659355-31659374 | None:intergenic | 35.0% | |
| ! | ACTTCAATATTAGGGCATCA+AGG | - | chr8_4:31659385-31659404 | Msa1323350:CDS | 35.0% |
| ! | GATCGTAAAGTGTCTCAACA+TGG | + | chr8_4:31658843-31658862 | None:intergenic | 40.0% |
| ! | TGGGAGTGTATACTGGATAA+GGG | + | chr8_4:31658881-31658900 | None:intergenic | 40.0% |
| ! | ATGGGAGTGTATACTGGATA+AGG | + | chr8_4:31658882-31658901 | None:intergenic | 40.0% |
| GCATTCAAGACAACTGTTGT+TGG | - | chr8_4:31658934-31658953 | Msa1323350:CDS | 40.0% | |
| TGCGCACATCCAAATAACTT+CGG | - | chr8_4:31659110-31659129 | Msa1323350:intron | 40.0% | |
| ! | TGAAACTTTTGGATCTGTGC+AGG | - | chr8_4:31659218-31659237 | Msa1323350:intron | 40.0% |
| ATGCACAACATGCAGATCAA+AGG | - | chr8_4:31659271-31659290 | Msa1323350:CDS | 40.0% | |
| AAAGGAATGCCACCTTTCAA+AGG | - | chr8_4:31659289-31659308 | Msa1323350:CDS | 40.0% | |
| AAGGAATGCCACCTTTCAAA+GGG | - | chr8_4:31659290-31659309 | Msa1323350:CDS | 40.0% | |
| !! | TTCTAGAACCCTTTGAAAGG+TGG | + | chr8_4:31659301-31659320 | None:intergenic | 40.0% |
| !! | AAAGGGTTCTAGAAACTGGA+AGG | - | chr8_4:31659307-31659326 | Msa1323350:CDS | 40.0% |
| !! | AAGGGTTCTAGAAACTGGAA+GGG | - | chr8_4:31659308-31659327 | Msa1323350:CDS | 40.0% |
| GAGCTGTTCAAGTCCCAAAC+AGG | + | chr8_4:31659190-31659209 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 31658814 | 31659416 | 31658814 | ID=Msa1323350;Name=Msa1323350 |
| chr8_4 | mRNA | 31658814 | 31659416 | 31658814 | ID=Msa1323350-mRNA-1;Parent=Msa1323350;Name=Msa1323350-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|0|0|1|1|1|2|0|121 |
| chr8_4 | exon | 31659228 | 31659416 | 31659228 | ID=Msa1323350-mRNA-1:exon:10293;Parent=Msa1323350-mRNA-1 |
| chr8_4 | exon | 31658814 | 31658990 | 31658814 | ID=Msa1323350-mRNA-1:exon:10292;Parent=Msa1323350-mRNA-1 |
| chr8_4 | CDS | 31659228 | 31659416 | 31659228 | ID=Msa1323350-mRNA-1:cds;Parent=Msa1323350-mRNA-1 |
| chr8_4 | CDS | 31658814 | 31658990 | 31658814 | ID=Msa1323350-mRNA-1:cds;Parent=Msa1323350-mRNA-1 |
| Gene Sequence |
| Protein sequence |
