AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1326830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1326830 MtrunA17_Chr4g0076091 96.104 539 19 1 25 561 6 544 0.0 1085
Msa1326830 MtrunA17_Chr5g0447691 57.570 535 215 6 34 559 12 543 0.0 603
Msa1326830 MtrunA17_Chr2g0290241 66.667 423 138 2 139 561 7 426 0.0 583
Msa1326830 MtrunA17_Chr5g0445331 53.175 504 221 8 68 559 31 531 3.69e-180 518
Msa1326830 MtrunA17_Chr3g0101141 48.168 546 243 8 34 559 16 541 7.63e-175 505
Msa1326830 MtrunA17_Chr2g0319091 49.787 470 183 4 135 559 117 578 1.01e-158 465
Msa1326830 MtrunA17_Chr1g0159461 47.838 370 192 1 190 559 223 591 5.08e-130 392
Msa1326830 MtrunA17_Chr3g0125811 40.514 506 257 9 87 559 75 569 1.32e-126 382
Msa1326830 MtrunA17_Chr7g0224271 36.181 398 223 5 182 561 290 674 7.56e-73 245
Msa1326830 MtrunA17_Chr2g0306641 33.484 442 260 10 130 559 67 486 1.11e-68 230
Msa1326830 MtrunA17_Chr3g0110971 33.943 383 223 8 192 559 219 586 7.60e-67 227
Msa1326830 MtrunA17_Chr8g0376381 34.409 372 219 9 193 559 170 521 1.20e-65 223
Msa1326830 MtrunA17_Chr7g0236851 35.714 378 222 7 190 559 292 656 1.21e-64 223
Msa1326830 MtrunA17_Chr4g0031721 32.000 375 253 2 187 560 311 684 1.13e-63 221
Msa1326830 MtrunA17_Chr7g0238891 31.016 374 256 2 187 559 367 739 1.19e-61 216
Msa1326830 MtrunA17_Chr4g0031731 32.283 381 242 7 187 557 249 623 3.11e-61 213
Msa1326830 MtrunA17_Chr4g0031711 31.347 386 245 8 187 560 356 733 4.24e-61 214
Msa1326830 MtrunA17_Chr2g0328391 31.070 383 248 6 187 560 294 669 7.02e-61 213
Msa1326830 MtrunA17_Chr4g0031701 31.759 381 244 7 187 557 249 623 8.85e-60 209
Msa1326830 MtrunA17_Chr2g0328381 30.319 376 254 4 187 559 270 640 9.30e-60 209
Msa1326830 MtrunA17_Chr2g0328341 31.217 378 250 6 187 560 267 638 3.13e-58 205
Msa1326830 MtrunA17_Chr4g0031741 32.718 379 247 6 185 560 277 650 1.47e-56 201
Msa1326830 MtrunA17_Chr2g0328411 29.474 380 263 4 181 559 278 653 2.82e-56 201
Msa1326830 MtrunA17_Chr7g0245601 34.521 365 223 7 196 559 442 791 1.86e-55 200
Msa1326830 MtrunA17_Chr2g0328431 29.952 414 264 9 155 560 259 654 2.35e-54 195
Msa1326830 MtrunA17_Chr6g0468561 32.208 385 230 10 190 559 293 661 3.35e-54 194
Msa1326830 MtrunA17_Chr8g0357601 32.216 388 235 8 187 559 177 551 3.64e-53 189
Msa1326830 MtrunA17_Chr2g0328401 29.859 355 242 5 183 535 281 630 3.94e-51 186
Msa1326830 MtrunA17_Chr4g0057891 30.670 388 246 7 187 561 144 521 4.92e-51 183
Msa1326830 MtrunA17_Chr3g0085681 27.338 417 246 6 193 559 26 435 1.18e-48 174
Msa1326830 MtrunA17_Chr2g0328361 29.737 380 226 10 187 559 269 614 1.68e-46 172
Msa1326830 MtrunA17_Chr4g0057291 29.793 386 249 10 187 560 287 662 5.27e-46 172
Msa1326830 MtrunA17_Chr4g0039661 33.143 350 217 10 216 559 216 554 2.80e-44 165
Msa1326830 MtrunA17_Chr1g0200401 31.220 410 235 15 179 559 67 458 5.45e-44 162
Msa1326830 MtrunA17_Chr1g0183641 34.783 299 177 8 267 561 194 478 1.18e-43 162
Msa1326830 MtrunA17_Chr4g0038761 31.165 369 234 9 196 559 88 441 1.41e-43 161
Msa1326830 MtrunA17_Chr5g0401131 29.275 386 245 11 192 559 114 489 1.95e-42 159
Msa1326830 MtrunA17_Chr5g0424131 30.704 355 226 9 221 561 152 500 9.83e-41 154
Msa1326830 MtrunA17_Chr3g0114841 29.213 356 225 7 224 561 155 501 6.07e-40 152
Msa1326830 MtrunA17_Chr2g0328371 33.333 252 157 5 271 516 5 251 7.38e-39 142
Msa1326830 MtrunA17_Chr3g0085361 28.024 339 188 5 270 559 18 349 3.67e-38 144
Msa1326830 MtrunA17_Chr2g0294461 26.598 391 265 8 182 559 203 584 1.13e-37 147
Msa1326830 MtrunA17_Chr2g0329591 28.827 392 242 11 191 559 73 450 5.07e-36 140
Msa1326830 MtrunA17_Chr4g0053901 27.316 421 247 10 189 559 56 467 7.89e-35 137
Msa1326830 MtrunA17_Chr2g0294471 24.422 389 276 8 182 559 194 575 3.56e-34 136
Msa1326830 MtrunA17_Chr5g0401421 25.412 425 252 10 189 559 32 445 2.25e-32 129
Msa1326830 MtrunA17_Chr7g0270031 24.566 346 245 8 221 559 217 553 3.57e-28 118
Msa1326830 MtrunA17_Chr4g0012071 25.066 379 251 10 193 561 197 552 1.63e-26 113
Msa1326830 MtrunA17_Chr5g0396491 31.364 220 138 5 192 406 56 267 5.43e-26 111
Msa1326830 MtrunA17_Chr5g0396491 32.886 149 99 1 412 559 329 477 5.10e-22 99.4
Msa1326830 MtrunA17_Chr7g0230731 24.672 381 251 9 193 561 373 729 6.13e-26 112
Msa1326830 MtrunA17_Chr8g0383981 25.628 398 258 14 177 560 123 496 2.98e-25 109
Msa1326830 MtrunA17_Chr4g0038851 31.628 215 139 3 187 400 43 250 1.87e-24 106
Msa1326830 MtrunA17_Chr4g0038851 34.228 149 97 1 412 559 320 468 6.03e-22 99.0
Msa1326830 MtrunA17_Chr7g0230771 24.409 381 252 9 193 561 376 732 3.54e-24 107
Msa1326830 MtrunA17_Chr1g0194621 24.069 349 242 8 224 559 179 517 1.61e-23 104
Msa1326830 MtrunA17_Chr8g0344101 25.000 400 249 14 190 559 221 599 1.02e-21 99.4
Msa1326830 MtrunA17_Chr8g0373561 23.575 386 262 12 188 561 178 542 4.37e-20 94.0
Msa1326830 MtrunA17_Chr2g0323111 24.719 178 126 3 387 560 133 306 8.30e-13 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1326830 AT4G17230.1 61.368 497 177 5 66 559 40 524 0.0 606
Msa1326830 AT4G17230.2 61.368 497 177 5 66 559 40 524 0.0 606
Msa1326830 AT5G48150.1 54.076 503 208 7 65 559 2 489 0.0 523
Msa1326830 AT5G48150.4 54.076 503 208 7 65 559 2 489 0.0 523
Msa1326830 AT5G48150.2 54.076 503 208 7 65 559 2 489 0.0 523
Msa1326830 AT5G48150.3 54.076 503 208 7 65 559 2 489 0.0 523
Msa1326830 AT1G50600.3 51.091 550 236 9 17 559 2 525 4.73e-175 505
Msa1326830 AT1G50600.2 50.903 554 239 9 13 559 73 600 2.48e-174 506
Msa1326830 AT1G50600.1 50.903 554 239 9 13 559 69 596 2.86e-174 506
Msa1326830 AT2G04890.1 55.961 411 169 4 149 559 14 412 1.50e-159 461
Msa1326830 AT1G21450.1 42.793 444 238 4 131 559 150 592 2.14e-129 391
Msa1326830 AT1G07530.1 33.867 375 245 3 188 560 392 765 1.13e-67 233
Msa1326830 AT3G03450.1 36.119 371 223 9 193 559 184 544 2.89e-67 228
Msa1326830 AT1G14920.1 34.127 378 216 9 193 559 173 528 2.02e-66 225
Msa1326830 AT1G66350.1 34.677 372 216 10 193 559 156 505 4.11e-66 224
Msa1326830 AT5G52510.1 36.311 347 196 7 228 559 303 639 1.65e-65 225
Msa1326830 AT2G01570.1 34.127 378 216 9 193 559 225 580 6.98e-63 217
Msa1326830 AT5G17490.1 33.777 376 227 9 189 559 157 515 3.49e-60 208
Msa1326830 AT5G59450.1 30.526 380 253 5 187 559 222 597 3.40e-59 207
Msa1326830 AT2G29060.2 31.579 380 249 4 188 560 280 655 3.66e-58 206
Msa1326830 AT2G29060.1 31.579 380 249 4 188 560 317 692 4.90e-58 206
Msa1326830 AT3G46600.2 30.108 372 257 3 189 559 1 370 8.89e-57 195
Msa1326830 AT3G46600.1 30.400 375 256 4 187 559 207 578 1.05e-56 200
Msa1326830 AT3G46600.3 30.214 374 258 3 187 559 175 546 1.82e-56 199
Msa1326830 AT1G07520.2 31.070 383 253 8 187 560 149 529 1.16e-55 196
Msa1326830 AT1G07520.4 31.070 383 253 8 187 560 149 529 1.16e-55 196
Msa1326830 AT1G07520.1 31.070 383 253 8 187 560 313 693 4.47e-55 197
Msa1326830 AT1G07520.3 31.070 383 253 8 187 560 314 694 4.76e-55 197
Msa1326830 AT2G37650.2 29.412 374 260 3 187 559 330 700 5.54e-53 192
Msa1326830 AT2G37650.1 29.412 374 260 3 187 559 342 712 6.01e-53 192
Msa1326830 AT3G54220.1 34.054 370 223 9 196 560 297 650 1.49e-51 187
Msa1326830 AT2G29065.1 30.287 383 257 7 187 560 247 628 2.14e-50 184
Msa1326830 AT5G66770.1 33.908 348 212 7 220 559 246 583 3.89e-50 182
Msa1326830 AT3G50650.1 32.092 349 219 7 220 559 202 541 2.42e-44 165
Msa1326830 AT5G41920.1 29.275 386 240 10 185 559 36 399 2.01e-42 157
Msa1326830 AT1G63100.1 30.398 352 220 6 226 560 286 629 2.63e-42 161
Msa1326830 AT1G63100.2 30.398 352 220 6 226 560 311 654 2.65e-42 161
Msa1326830 AT4G37650.1 29.306 389 248 10 192 559 146 528 1.21e-39 152
Msa1326830 AT1G55580.1 30.421 309 191 7 266 559 145 444 2.72e-39 149
Msa1326830 AT3G49950.1 26.992 389 258 12 189 559 27 407 5.98e-35 136
Msa1326830 AT4G08250.1 26.706 337 239 5 227 559 144 476 3.16e-34 135
Msa1326830 AT3G60630.1 27.011 348 227 9 226 561 291 623 4.09e-27 116
Msa1326830 AT1G50420.1 32.558 215 135 3 187 398 52 259 1.00e-26 114
Msa1326830 AT1G50420.1 33.775 151 99 1 412 561 330 480 1.45e-23 104
Msa1326830 AT3G13840.1 26.316 361 229 13 215 559 170 509 2.00e-23 104
Msa1326830 AT2G45160.1 26.912 353 216 12 226 561 313 640 4.23e-23 103
Msa1326830 AT4G00150.1 23.195 457 302 14 116 561 137 555 4.15e-20 94.4
Msa1326830 AT4G36710.1 25.193 389 251 13 187 559 120 484 1.23e-19 92.4
Msa1326830 AT5G67411.1 29.146 199 128 6 310 497 2 198 8.64e-18 82.4

Find 143 sgRNAs with CRISPR-Local

Find 251 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAGTCCTTCAACATATTT+TGG 0.122383 8_4:-41950455 None:intergenic
CTCCATCAGCTAAATATAAT+TGG 0.146253 8_4:+41949392 Msa1326830:CDS
TCTATGAAACCGGTGATTTC+TGG 0.156389 8_4:-41949109 None:intergenic
AGGACTTCCATCAAAATTAT+AGG 0.229164 8_4:+41950469 Msa1326830:CDS
ACCCCTGTTATTCGAATAAA+AGG 0.251389 8_4:-41949859 None:intergenic
AATCCGCTTCTTATCGTCTT+TGG 0.262124 8_4:-41950271 None:intergenic
ATGTATGCAAACTTAAAATA+TGG 0.291227 8_4:-41949706 None:intergenic
CACTGGACATACCGCTTAAC+GGG 0.299460 8_4:-41949246 None:intergenic
AAACCGAGAGCAGCTGAATT+TGG 0.309152 8_4:-41948962 None:intergenic
TCCTTTCTTTCAAAGGTTTG+TGG 0.333187 8_4:+41950197 Msa1326830:CDS
TGAGACTCTTGTGGAGAAAA+TGG 0.334739 8_4:-41949175 None:intergenic
GGTGAAGCGTTGCTGCTTGT+AGG 0.359696 8_4:-41949074 None:intergenic
TGATTTCTGGGTATGATTCC+AGG 0.361344 8_4:-41949096 None:intergenic
CAGCACATAGATTGGTTTGT+AGG 0.383859 8_4:-41949012 None:intergenic
CTCTTCTGCTTGGACGTGTT+AGG 0.389557 8_4:+41950548 Msa1326830:CDS
CAAACTAAAGGGAAAATTTA+CGG 0.391093 8_4:-41950052 None:intergenic
AAAATCAATTATATGAATCC+TGG 0.393012 8_4:-41949770 None:intergenic
TTTCTATCACCAACCTGCTC+AGG 0.406462 8_4:+41948982 Msa1326830:CDS
GTGAAGATGGTATCGGTTGC+TGG 0.411075 8_4:+41949532 Msa1326830:CDS
AGAAATAGAGAATTCATTGC+TGG 0.413761 8_4:+41949303 Msa1326830:CDS
AACGACTTTCAAACTTTGCC+AGG 0.417891 8_4:+41949935 Msa1326830:CDS
TAGAGACAGATTGTTGAGAT+TGG 0.422530 8_4:+41950113 Msa1326830:CDS
CTATGAAACCGGTGATTTCT+GGG 0.422823 8_4:-41949108 None:intergenic
CGGCATATGGTGCAAACTAA+AGG 0.425793 8_4:-41950064 None:intergenic
GGTGTGTTTGAGTTAGAAAC+TGG 0.432248 8_4:-41947897 None:intergenic
TTTGATATTGTTGACAGTTA+CGG 0.438331 8_4:+41949337 Msa1326830:CDS
TTCTGTCCAAAATATGTTGA+AGG 0.440203 8_4:+41950449 Msa1326830:CDS
ACACTGGACATACCGCTTAA+CGG 0.441649 8_4:-41949247 None:intergenic
TCAACAAGGGTAACAACCTT+TGG 0.445568 8_4:-41950150 None:intergenic
GAGGTTAAGTGCTTACTTGT+TGG 0.446416 8_4:+41949567 Msa1326830:CDS
TCCACAAACCTTTGAAAGAA+AGG 0.448633 8_4:-41950198 None:intergenic
CAGAGCAGAACTGCGTGGCC+CGG 0.448928 8_4:+41950298 Msa1326830:CDS
TCCATCAGCTAAATATAATT+GGG 0.450403 8_4:+41949393 Msa1326830:CDS
GCTACCACCAGCACATAGAT+TGG 0.452385 8_4:-41949020 None:intergenic
AGAGATGGTGCTCTCTATCT+TGG 0.454465 8_4:+41950501 Msa1326830:CDS
TGCTGGTGGTAGCAGCAGCC+AGG 0.459468 8_4:+41949030 Msa1326830:CDS
TTCCACAGCGCTGCAATGTC+TGG 0.460279 8_4:+41949979 Msa1326830:CDS
TTGAAAGAAAGGAGAAGTAT+TGG 0.462842 8_4:-41950187 None:intergenic
TCGAATAAAAGGTGGTCCAC+CGG 0.467899 8_4:-41949848 None:intergenic
GAAGCGCTTGCACGCAGGCC+CGG 0.469418 8_4:+41949829 Msa1326830:CDS
TCCATCCAGCCTCTTGCTGT+TGG 0.472205 8_4:-41949499 None:intergenic
TGTCTGTCTGTAGCTTTGCT+TGG 0.473325 8_4:-41947918 None:intergenic
GCATCACTGAAGAACTCAAT+TGG 0.473571 8_4:-41950425 None:intergenic
TTACACTCTCATCCGGCATA+TGG 0.477901 8_4:-41950077 None:intergenic
AGAGCAGAACTGCGTGGCCC+GGG 0.478609 8_4:+41950299 Msa1326830:CDS
CTTTAGTTTGCACCATATGC+CGG 0.480501 8_4:+41950065 Msa1326830:CDS
GGCATATGGTGCAAACTAAA+GGG 0.480691 8_4:-41950063 None:intergenic
GTCATTAGCTCTTTGCTCGT+TGG 0.480715 8_4:-41949655 None:intergenic
TGTAGGTAAATGCCCTGAGC+AGG 0.480980 8_4:-41948995 None:intergenic
TCCCAATTATATTTAGCTGA+TGG 0.481678 8_4:-41949394 None:intergenic
TAAGCACTTAACCTCTGGAT+TGG 0.483912 8_4:-41949559 None:intergenic
ATACTTCTCCTTTCTTTCAA+AGG 0.491697 8_4:+41950190 Msa1326830:CDS
AGGGAGTTGAGAGGGTGGAG+AGG 0.494885 8_4:+41950346 Msa1326830:CDS
GGTGGAGGACTTCAGATTGT+AGG 0.496448 8_4:+41949910 Msa1326830:CDS
CGAAAGAAAGATCAGTTCCC+TGG 0.499255 8_4:-41949048 None:intergenic
ATATTCCAACAGCAAGAGGC+TGG 0.499931 8_4:+41949494 Msa1326830:CDS
TCATTTCATGCACGCGGTGG+AGG 0.506284 8_4:+41949895 Msa1326830:CDS
CTATCATGTTGACGATGTCC+CGG 0.506811 8_4:-41950317 None:intergenic
CGAATAAAAGGTGGTCCACC+GGG 0.511303 8_4:-41949847 None:intergenic
CTATGGCCACCTCTTCTGCT+TGG 0.512179 8_4:+41950538 Msa1326830:CDS
AAGCAATGGCAAATGAATCC+AGG 0.513079 8_4:+41949752 Msa1326830:CDS
GGATAATGTATTGGTGAAGA+TGG 0.513962 8_4:+41949519 Msa1326830:CDS
ACAAGTAAGCACTTAACCTC+TGG 0.514019 8_4:-41949564 None:intergenic
CATTTCAAAGCTTGACATGA+AGG 0.514416 8_4:+41949432 Msa1326830:CDS
CGACTTGTAGATCAAGCTTC+CGG 0.514475 8_4:-41949617 None:intergenic
TTAAGTGCTTACTTGTTGGA+AGG 0.516488 8_4:+41949571 Msa1326830:CDS
CACCCTCTCAACTCCCTCAC+AGG 0.518699 8_4:-41950340 None:intergenic
GAAAGGAGAAGTATTGGTGT+TGG 0.519467 8_4:-41950181 None:intergenic
AGAATCACAGTCTATGAAAC+CGG 0.522986 8_4:-41949119 None:intergenic
TATCATGTTGACGATGTCCC+GGG 0.523589 8_4:-41950316 None:intergenic
CAATCATCTGACAACACCTA+TGG 0.525163 8_4:+41949220 Msa1326830:CDS
AAACAGAACTCCACGAGACC+TGG 0.526798 8_4:-41949953 None:intergenic
AGGCCAAATTCAGCTGCTCT+CGG 0.528218 8_4:+41948959 Msa1326830:CDS
TCAAACTTTGCCAGGTCTCG+TGG 0.529305 8_4:+41949943 Msa1326830:CDS
ATCATTCCCATCGTCAACAC+TGG 0.531358 8_4:-41949263 None:intergenic
ATGGTGCTCTCTATCTTGGT+TGG 0.532409 8_4:+41950505 Msa1326830:CDS
ACCGCTTAACGGGGATCCAT+AGG 0.533680 8_4:-41949236 None:intergenic
GGTTAAGAGTTTGTCGCCAA+AGG 0.537523 8_4:+41950134 Msa1326830:CDS
TTTACGGCGAGGGCTTCTCC+AGG 0.538623 8_4:-41950036 None:intergenic
GAAATAGAGAATTCATTGCT+GGG 0.540647 8_4:+41949304 Msa1326830:CDS
CATAGGTGTTGTCAGATGAT+TGG 0.540781 8_4:-41949219 None:intergenic
CTACAAACCAATCTATGTGC+TGG 0.543214 8_4:+41949013 Msa1326830:CDS
AGAGCAAGATTAGAATCGTC+CGG 0.543631 8_4:+41949598 Msa1326830:CDS
TGTGCACAAGCTGTCTATGA+TGG 0.545177 8_4:+41949469 Msa1326830:CDS
GAATTCATTGCTGGGGCCTG+AGG 0.545314 8_4:+41949312 Msa1326830:CDS
TGTTGGATTCTTGCTCAACA+AGG 0.545794 8_4:-41950164 None:intergenic
GATTTCTGGGTATGATTCCA+GGG 0.546662 8_4:-41949095 None:intergenic
TCCAACAGCAAGAGGCTGGA+TGG 0.548787 8_4:+41949498 Msa1326830:CDS
GAAAATCTTAGAGTTAGTCC+TGG 0.549759 8_4:+41950018 Msa1326830:CDS
TCTATGCTTACACTCTCATC+CGG 0.551650 8_4:-41950084 None:intergenic
AAAGGGAAAATTTACGGCGA+GGG 0.556638 8_4:-41950046 None:intergenic
AGGCTGGATGGATAATGTAT+TGG 0.564066 8_4:+41949510 Msa1326830:CDS
TCAAAGGTTTGTGGAGACAA+TGG 0.564383 8_4:+41950206 Msa1326830:CDS
TGAGTTAGAAACTGGATTGG+TGG 0.564532 8_4:-41947889 None:intergenic
TAAGTGCTTACTTGTTGGAA+GGG 0.566804 8_4:+41949572 Msa1326830:CDS
TGACAGTTACGGCAGCTGCA+TGG 0.566890 8_4:+41949348 Msa1326830:CDS
AAGCAAAGCTACAGACAGAC+AGG 0.571601 8_4:+41947920 Msa1326830:CDS
CAGCTTGTGCACACAGCAAG+AGG 0.573250 8_4:-41949459 None:intergenic
TTCTATCACCAACCTGCTCA+GGG 0.576219 8_4:+41948983 Msa1326830:CDS
TCAACAATATCAAAATCCTC+AGG 0.578052 8_4:-41949328 None:intergenic
GCTGGTGGTAGCAGCAGCCA+GGG 0.581099 8_4:+41949031 Msa1326830:CDS
GTTTGAGTTAGAAACTGGAT+TGG 0.584482 8_4:-41947892 None:intergenic
GTTAGAAACTGGATTGGTGG+TGG 0.588492 8_4:-41947886 None:intergenic
GTTAGATGATACACTGGCAT+AGG 0.594598 8_4:-41949146 None:intergenic
TGCATGGAGACTAGCCTCCA+CGG 0.598856 8_4:+41949364 Msa1326830:CDS
GATGATACACTGGCATAGGA+AGG 0.603436 8_4:-41949142 None:intergenic
GGTGATATTCCAACAGCAAG+AGG 0.604701 8_4:+41949490 Msa1326830:CDS
CAAGCAGCAACGCTTCACCC+TGG 0.609227 8_4:+41949078 Msa1326830:CDS
GCATTGTCTAAATCCTGCCA+TGG 0.611749 8_4:-41950403 None:intergenic
GTATGTCCAGTGTTGACGAT+GGG 0.612286 8_4:+41949257 Msa1326830:CDS
CAATCATTTCATGCACGCGG+TGG 0.615401 8_4:+41949892 Msa1326830:CDS
CAAACCAATCTATGTGCTGG+TGG 0.616086 8_4:+41949016 Msa1326830:CDS
GTTGGATTCTTGCTCAACAA+GGG 0.617090 8_4:-41950163 None:intergenic
CTGTGAGGGAGTTGAGAGGG+TGG 0.617736 8_4:+41950341 Msa1326830:CDS
CTAACACGTCCAAGCAGAAG+AGG 0.617768 8_4:-41950547 None:intergenic
GAATCATACCCAGAAATCAC+CGG 0.623101 8_4:+41949100 Msa1326830:CDS
AGGTGTTGTCAGATGATTGG+TGG 0.624480 8_4:-41949216 None:intergenic
TATAGGCTTGAACATAGAGA+TGG 0.624582 8_4:+41950486 Msa1326830:CDS
TGGTTGGATGAAGAGAGCTA+TGG 0.630176 8_4:+41950521 Msa1326830:CDS
TGGCATGATTGAGACTCTTG+TGG 0.635813 8_4:-41949184 None:intergenic
CGTCAACATGATAGCCTGTG+AGG 0.636782 8_4:+41950326 Msa1326830:CDS
GTCAACATGATAGCCTGTGA+GGG 0.638925 8_4:+41950327 Msa1326830:CDS
AGCCTGTGAGGGAGTTGAGA+GGG 0.643951 8_4:+41950338 Msa1326830:CDS
AGTCTTGTTAGATGATACAC+TGG 0.645472 8_4:-41949152 None:intergenic
GGTATGTCCAGTGTTGACGA+TGG 0.649722 8_4:+41949256 Msa1326830:CDS
TGTATTGGTGAAGATGGTAT+CGG 0.655186 8_4:+41949525 Msa1326830:CDS
GCGCCAAAGACGATAAGAAG+CGG 0.655603 8_4:+41950268 Msa1326830:CDS
TAAAGGGAAAATTTACGGCG+AGG 0.656398 8_4:-41950047 None:intergenic
CCTGTTATTCGAATAAAAGG+TGG 0.661170 8_4:-41949856 None:intergenic
ACACGTCCAAGCAGAAGAGG+TGG 0.661453 8_4:-41950544 None:intergenic
AACCAGACATTGCAGCGCTG+TGG 0.666708 8_4:-41949981 None:intergenic
TCACAATCATTTCATGCACG+CGG 0.666725 8_4:+41949889 Msa1326830:CDS
AAATAGAGAATTCATTGCTG+GGG 0.674391 8_4:+41949305 Msa1326830:CDS
TTATTGAAGCGCTTGCACGC+AGG 0.680778 8_4:+41949824 Msa1326830:CDS
ACTGGACATACCGCTTAACG+GGG 0.686166 8_4:-41949245 None:intergenic
GGTAAATGCCCTGAGCAGGT+TGG 0.700911 8_4:-41948991 None:intergenic
CTGATGGAGATGCTCCGTGG+AGG 0.702180 8_4:-41949378 None:intergenic
TAGCTGATGGAGATGCTCCG+TGG 0.705467 8_4:-41949381 None:intergenic
AAATCGCACAGGGAACACAA+TGG 0.711979 8_4:+41949794 Msa1326830:CDS
TTGCTGGAGATCCAATCCAG+AGG 0.713173 8_4:+41949548 Msa1326830:CDS
TAGTGCAGAGCAGAACTGCG+TGG 0.725432 8_4:+41950293 Msa1326830:CDS
TAGCCTGTGAGGGAGTTGAG+AGG 0.733893 8_4:+41950337 Msa1326830:CDS
ATGATTGGTGGTGATCTGAG+TGG 0.759324 8_4:-41949204 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAATTTATAATATTGAAT+AGG - chr8_4:41948141-41948160 None:intergenic 10.0%
!! ATAACAAAATAAAATTGTTA+AGG - chr8_4:41948692-41948711 None:intergenic 10.0%
!! AAAAAAAAAAATAAGTAGAG+TGG - chr8_4:41947020-41947039 None:intergenic 15.0%
!! GAAAGAAAAAAACATAATTA+GGG - chr8_4:41947122-41947141 None:intergenic 15.0%
!! AGAAAGAAAAAAACATAATT+AGG - chr8_4:41947123-41947142 None:intergenic 15.0%
!! TAAATCACATATAATTATTG+TGG + chr8_4:41947142-41947161 Msa1326830:intron 15.0%
!! ATATAATTATTGTGGTAAAA+TGG + chr8_4:41947150-41947169 Msa1326830:intron 15.0%
!! ATTTAGTGTTATATTGTATT+GGG - chr8_4:41947433-41947452 None:intergenic 15.0%
!! TATTTAGTGTTATATTGTAT+TGG - chr8_4:41947434-41947453 None:intergenic 15.0%
!!! AAGAAAAGTGTATAAATAAA+GGG - chr8_4:41947498-41947517 None:intergenic 15.0%
!!! TAAGAAAAGTGTATAAATAA+AGG - chr8_4:41947499-41947518 None:intergenic 15.0%
!!! AAAGCATTTGATTTAATATA+AGG - chr8_4:41947773-41947792 None:intergenic 15.0%
!!! TTAAAATTAATCTTAGTCTT+AGG + chr8_4:41947964-41947983 Msa1326830:intron 15.0%
!!! TTTTCAATTGAGAAATTTAA+AGG + chr8_4:41948192-41948211 Msa1326830:intron 15.0%
!! TTTCAATTGAGAAATTTAAA+GGG + chr8_4:41948193-41948212 Msa1326830:intron 15.0%
!! AATCTAATAGTAAAGAAAAA+AGG - chr8_4:41948868-41948887 None:intergenic 15.0%
!! TCTTGAAATAATAATTTACT+CGG - chr8_4:41948922-41948941 None:intergenic 15.0%
!! AAAAAAAGTTACTGCAAAAA+AGG + chr8_4:41947191-41947210 Msa1326830:intron 20.0%
!!! GACACTTTTTTTTAGAAAAT+AGG - chr8_4:41947364-41947383 None:intergenic 20.0%
!!! TGAAATGAATCAGTTATTTT+GGG - chr8_4:41947386-41947405 None:intergenic 20.0%
!!! TTGAAATGAATCAGTTATTT+TGG - chr8_4:41947387-41947406 None:intergenic 20.0%
!! AAAGTGGAATATATTGTATT+GGG - chr8_4:41947474-41947493 None:intergenic 20.0%
!!! AGAAAAGTGTATAAATAAAG+GGG - chr8_4:41947497-41947516 None:intergenic 20.0%
!! GGAAAGTTCAAAAAAATAAA+GGG - chr8_4:41948019-41948038 None:intergenic 20.0%
!! TGGAAAGTTCAAAAAAATAA+AGG - chr8_4:41948020-41948039 None:intergenic 20.0%
!!! GATTCTTTTCTTTCTTTTTA+TGG - chr8_4:41948040-41948059 None:intergenic 20.0%
!!! TGAGATATGAGAAATTATTT+TGG + chr8_4:41948272-41948291 Msa1326830:intron 20.0%
!! AAGATTCTTTCTATGTAAAA+TGG - chr8_4:41948331-41948350 None:intergenic 20.0%
!!! TTATTTTCTCGAAATAGTAT+CGG - chr8_4:41948361-41948380 None:intergenic 20.0%
!! GTGATTAAATGTAATCAAAT+TGG + chr8_4:41948383-41948402 Msa1326830:intron 20.0%
!! ACATAGTATATATAGATTTC+TGG + chr8_4:41948442-41948461 Msa1326830:intron 20.0%
!! GATTCTAAATCAAAATCAAA+TGG - chr8_4:41948531-41948550 None:intergenic 20.0%
!!! ACAATTTTATTTTGTTATGC+AGG + chr8_4:41948694-41948713 Msa1326830:intron 20.0%
!! ATGTATGCAAACTTAAAATA+TGG - chr8_4:41949709-41949728 None:intergenic 20.0%
!! AAAATCAATTATATGAATCC+TGG - chr8_4:41949773-41949792 None:intergenic 20.0%
! TCAATTTCACCATTTACTAA+AGG + chr8_4:41947084-41947103 Msa1326830:intron 25.0%
!! TCATTTTCTCCTTTAGTAAA+TGG - chr8_4:41947096-41947115 None:intergenic 25.0%
! TACTTACAACAAATATCACA+TGG - chr8_4:41947284-41947303 None:intergenic 25.0%
! GAAAGTGGAATATATTGTAT+TGG - chr8_4:41947475-41947494 None:intergenic 25.0%
!! TAAGAACTATGATTTGGATT+TGG - chr8_4:41947816-41947835 None:intergenic 25.0%
! AAATCCAAATCATAGTTCTT+AGG + chr8_4:41947815-41947834 Msa1326830:intron 25.0%
!!! GATTAATTTTAAGAAGTGTC+TGG - chr8_4:41947956-41947975 None:intergenic 25.0%
! AATCATGTAATATGTGAAAC+TGG + chr8_4:41948056-41948075 Msa1326830:intron 25.0%
! TCAATTGAAAATTCATCATC+AGG - chr8_4:41948183-41948202 None:intergenic 25.0%
!!! AATTATTTTGGATCCTCAAT+TGG + chr8_4:41948284-41948303 Msa1326830:intron 25.0%
!!! TGTGTATCTCTTTTGTTATA+TGG + chr8_4:41948503-41948522 Msa1326830:intron 25.0%
! TTTGATATTGTTGACAGTTA+CGG + chr8_4:41949337-41949356 Msa1326830:CDS 25.0%
! TCCATCAGCTAAATATAATT+GGG + chr8_4:41949393-41949412 Msa1326830:CDS 25.0%
! CAAACTAAAGGGAAAATTTA+CGG - chr8_4:41950055-41950074 None:intergenic 25.0%
TACAACAAATATCACATGGT+TGG - chr8_4:41947280-41947299 None:intergenic 30.0%
TAAGTATTTAAAGAACGTCC+CGG + chr8_4:41947329-41947348 Msa1326830:intron 30.0%
AGAAAATAGGTCAGTTATTC+CGG - chr8_4:41947351-41947370 None:intergenic 30.0%
TGTATAAATAAAGGGGAAAG+TGG - chr8_4:41947490-41947509 None:intergenic 30.0%
CCAAAGTATGTCAATTAAAC+CGG + chr8_4:41947533-41947552 Msa1326830:intron 30.0%
CAAAGTATGTCAATTAAACC+GGG + chr8_4:41947534-41947553 Msa1326830:intron 30.0%
!! AGTTGAATACTGTTTGGTTT+TGG - chr8_4:41947647-41947666 None:intergenic 30.0%
AGCTATAGTCTACGTATAAT+AGG - chr8_4:41947739-41947758 None:intergenic 30.0%
! CTATGATTTGGATTTGGATT+TGG - chr8_4:41947810-41947829 None:intergenic 30.0%
ATAGTTCTTAGGTGAGATTT+AGG + chr8_4:41947826-41947845 Msa1326830:intron 30.0%
TGAAAATTCATCATCAGGTA+AGG - chr8_4:41948178-41948197 None:intergenic 30.0%
CAAAATCTGAATTCCAATTG+AGG - chr8_4:41948300-41948319 None:intergenic 30.0%
GATTAGTACTAGATTACTAG+TGG + chr8_4:41948464-41948483 Msa1326830:intron 30.0%
GAAGATATGTTCAAGCTAAA+TGG - chr8_4:41948592-41948611 None:intergenic 30.0%
AGACATTTGTCAATTGTAGT+TGG + chr8_4:41948730-41948749 Msa1326830:intron 30.0%
GAAATAATAATTTACTCGGC+CGG - chr8_4:41948918-41948937 None:intergenic 30.0%
AGAAATAGAGAATTCATTGC+TGG + chr8_4:41949303-41949322 Msa1326830:CDS 30.0%
GAAATAGAGAATTCATTGCT+GGG + chr8_4:41949304-41949323 Msa1326830:CDS 30.0%
AAATAGAGAATTCATTGCTG+GGG + chr8_4:41949305-41949324 Msa1326830:CDS 30.0%
TCAACAATATCAAAATCCTC+AGG - chr8_4:41949331-41949350 None:intergenic 30.0%
CTCCATCAGCTAAATATAAT+TGG + chr8_4:41949392-41949411 Msa1326830:CDS 30.0%
TCCCAATTATATTTAGCTGA+TGG - chr8_4:41949397-41949416 None:intergenic 30.0%
!!! GTCAAGCTTTGAAATGTTTT+CGG - chr8_4:41949428-41949447 None:intergenic 30.0%
!!! TGCTGTTATTTTTGAAGCAA+TGG + chr8_4:41949738-41949757 Msa1326830:CDS 30.0%
!! AATTGATTTTCAAATCGCAC+AGG + chr8_4:41949783-41949802 Msa1326830:CDS 30.0%
!! ATTGATTTTCAAATCGCACA+GGG + chr8_4:41949784-41949803 Msa1326830:CDS 30.0%
! CACCTTTTATTCGAATAACA+GGG + chr8_4:41949857-41949876 Msa1326830:CDS 30.0%
! ACCTTTTATTCGAATAACAG+GGG + chr8_4:41949858-41949877 Msa1326830:CDS 30.0%
! TTGAAAGAAAGGAGAAGTAT+TGG - chr8_4:41950190-41950209 None:intergenic 30.0%
ATACTTCTCCTTTCTTTCAA+AGG + chr8_4:41950190-41950209 Msa1326830:CDS 30.0%
TTCTGTCCAAAATATGTTGA+AGG + chr8_4:41950449-41950468 Msa1326830:CDS 30.0%
AGGACTTCCATCAAAATTAT+AGG + chr8_4:41950469-41950488 Msa1326830:CDS 30.0%
! GTTCAAGCCTATAATTTTGA+TGG - chr8_4:41950479-41950498 None:intergenic 30.0%
GAAAATAGGTCAGTTATTCC+GGG - chr8_4:41947350-41947369 None:intergenic 35.0%
CCGGTTTAATTGACATACTT+TGG - chr8_4:41947536-41947555 None:intergenic 35.0%
!!! ATTACTCGTTTTTGAAGCTG+AGG + chr8_4:41947577-41947596 Msa1326830:intron 35.0%
TTGAAACAAAGGAAGGAACT+TGG - chr8_4:41947609-41947628 None:intergenic 35.0%
CCTTGAATTGAAACAAAGGA+AGG - chr8_4:41947616-41947635 None:intergenic 35.0%
! CCTTCCTTTGTTTCAATTCA+AGG + chr8_4:41947613-41947632 Msa1326830:intron 35.0%
! GTAGCCTTGAATTGAAACAA+AGG - chr8_4:41947620-41947639 None:intergenic 35.0%
!!! AATACTGTTTGGTTTTGGTG+GGG - chr8_4:41947642-41947661 None:intergenic 35.0%
!!! GAATACTGTTTGGTTTTGGT+GGG - chr8_4:41947643-41947662 None:intergenic 35.0%
!!! TGAATACTGTTTGGTTTTGG+TGG - chr8_4:41947644-41947663 None:intergenic 35.0%
CTCACCTAAGAACTATGATT+TGG - chr8_4:41947822-41947841 None:intergenic 35.0%
! GTTTGAGTTAGAAACTGGAT+TGG - chr8_4:41947895-41947914 None:intergenic 35.0%
ACAACGACACTTCATATTGA+AGG - chr8_4:41948414-41948433 None:intergenic 35.0%
CATCTAACTACTTGCCAAAA+TGG + chr8_4:41948765-41948784 Msa1326830:intron 35.0%
!! TTTCTGAGGAATCACCATTT+TGG - chr8_4:41948782-41948801 None:intergenic 35.0%
AGAATCACAGTCTATGAAAC+CGG - chr8_4:41949122-41949141 None:intergenic 35.0%
!! AGTCTTGTTAGATGATACAC+TGG - chr8_4:41949155-41949174 None:intergenic 35.0%
CATTTCAAAGCTTGACATGA+AGG + chr8_4:41949432-41949451 Msa1326830:CDS 35.0%
! GGATAATGTATTGGTGAAGA+TGG + chr8_4:41949519-41949538 Msa1326830:CDS 35.0%
! TGTATTGGTGAAGATGGTAT+CGG + chr8_4:41949525-41949544 Msa1326830:CDS 35.0%
! TTAAGTGCTTACTTGTTGGA+AGG + chr8_4:41949571-41949590 Msa1326830:CDS 35.0%
! TAAGTGCTTACTTGTTGGAA+GGG + chr8_4:41949572-41949591 Msa1326830:CDS 35.0%
CCTGTTATTCGAATAAAAGG+TGG - chr8_4:41949859-41949878 None:intergenic 35.0%
! CCACCTTTTATTCGAATAAC+AGG + chr8_4:41949856-41949875 Msa1326830:CDS 35.0%
ACCCCTGTTATTCGAATAAA+AGG - chr8_4:41949862-41949881 None:intergenic 35.0%
GAAAATCTTAGAGTTAGTCC+TGG + chr8_4:41950018-41950037 Msa1326830:CDS 35.0%
TAGAGACAGATTGTTGAGAT+TGG + chr8_4:41950113-41950132 Msa1326830:CDS 35.0%
TCCTTTCTTTCAAAGGTTTG+TGG + chr8_4:41950197-41950216 Msa1326830:CDS 35.0%
! TCCACAAACCTTTGAAAGAA+AGG - chr8_4:41950201-41950220 None:intergenic 35.0%
!! GGAGACAATGGATTTTTACA+CGG + chr8_4:41950218-41950237 Msa1326830:CDS 35.0%
!! ATGAAGTTTTTGGAAAGTGG+AGG + chr8_4:41950370-41950389 Msa1326830:CDS 35.0%
! GGAAGTCCTTCAACATATTT+TGG - chr8_4:41950458-41950477 None:intergenic 35.0%
TATAGGCTTGAACATAGAGA+TGG + chr8_4:41950486-41950505 Msa1326830:CDS 35.0%
TGGGTGCAAAATCAAAACTC+AGG + chr8_4:41946978-41946997 Msa1326830:intron 40.0%
GTATGTCAATTAAACCGGGA+CGG + chr8_4:41947538-41947557 Msa1326830:intron 40.0%
!! CGGTTGAGTTGAATACTGTT+TGG - chr8_4:41947653-41947672 None:intergenic 40.0%
TGAGTAGAAGATTCCTCACT+CGG - chr8_4:41947678-41947697 None:intergenic 40.0%
! TGAGTTAGAAACTGGATTGG+TGG - chr8_4:41947892-41947911 None:intergenic 40.0%
! GGTGTGTTTGAGTTAGAAAC+TGG - chr8_4:41947900-41947919 None:intergenic 40.0%
AGCATAGCAGTAGATTTCTG+AGG - chr8_4:41948796-41948815 None:intergenic 40.0%
AAGGTTATTCCCAACAGAAC+TGG - chr8_4:41948849-41948868 None:intergenic 40.0%
! GCTATTCCGATTTCCTTTTC+CGG + chr8_4:41948896-41948915 Msa1326830:intron 40.0%
ATAATTTACTCGGCCGGAAA+AGG - chr8_4:41948912-41948931 None:intergenic 40.0%
AGATGCAAATGTCTCAGCAA+AGG + chr8_4:41948939-41948958 Msa1326830:intron 40.0%
!! CAGCACATAGATTGGTTTGT+AGG - chr8_4:41949015-41949034 None:intergenic 40.0%
CTACAAACCAATCTATGTGC+TGG + chr8_4:41949013-41949032 Msa1326830:CDS 40.0%
! GATTTCTGGGTATGATTCCA+GGG - chr8_4:41949098-41949117 None:intergenic 40.0%
! TGATTTCTGGGTATGATTCC+AGG - chr8_4:41949099-41949118 None:intergenic 40.0%
GAATCATACCCAGAAATCAC+CGG + chr8_4:41949100-41949119 Msa1326830:CDS 40.0%
CTATGAAACCGGTGATTTCT+GGG - chr8_4:41949111-41949130 None:intergenic 40.0%
TCTATGAAACCGGTGATTTC+TGG - chr8_4:41949112-41949131 None:intergenic 40.0%
GTTAGATGATACACTGGCAT+AGG - chr8_4:41949149-41949168 None:intergenic 40.0%
TGAGACTCTTGTGGAGAAAA+TGG - chr8_4:41949178-41949197 None:intergenic 40.0%
! CATAGGTGTTGTCAGATGAT+TGG - chr8_4:41949222-41949241 None:intergenic 40.0%
CAATCATCTGACAACACCTA+TGG + chr8_4:41949220-41949239 Msa1326830:CDS 40.0%
AGGCTGGATGGATAATGTAT+TGG + chr8_4:41949510-41949529 Msa1326830:CDS 40.0%
TAAGCACTTAACCTCTGGAT+TGG - chr8_4:41949562-41949581 None:intergenic 40.0%
ACAAGTAAGCACTTAACCTC+TGG - chr8_4:41949567-41949586 None:intergenic 40.0%
GAGGTTAAGTGCTTACTTGT+TGG + chr8_4:41949567-41949586 Msa1326830:CDS 40.0%
AGAGCAAGATTAGAATCGTC+CGG + chr8_4:41949598-41949617 Msa1326830:CDS 40.0%
AAGCAATGGCAAATGAATCC+AGG + chr8_4:41949752-41949771 Msa1326830:CDS 40.0%
TCACAATCATTTCATGCACG+CGG + chr8_4:41949889-41949908 Msa1326830:CDS 40.0%
AACGACTTTCAAACTTTGCC+AGG + chr8_4:41949935-41949954 Msa1326830:CDS 40.0%
AAAGGGAAAATTTACGGCGA+GGG - chr8_4:41950049-41950068 None:intergenic 40.0%
TAAAGGGAAAATTTACGGCG+AGG - chr8_4:41950050-41950069 None:intergenic 40.0%
GGCATATGGTGCAAACTAAA+GGG - chr8_4:41950066-41950085 None:intergenic 40.0%
!! CTTTAGTTTGCACCATATGC+CGG + chr8_4:41950065-41950084 Msa1326830:CDS 40.0%
TCTATGCTTACACTCTCATC+CGG - chr8_4:41950087-41950106 None:intergenic 40.0%
TCAACAAGGGTAACAACCTT+TGG - chr8_4:41950153-41950172 None:intergenic 40.0%
GTTGGATTCTTGCTCAACAA+GGG - chr8_4:41950166-41950185 None:intergenic 40.0%
TGTTGGATTCTTGCTCAACA+AGG - chr8_4:41950167-41950186 None:intergenic 40.0%
!! GAAAGGAGAAGTATTGGTGT+TGG - chr8_4:41950184-41950203 None:intergenic 40.0%
TCAAAGGTTTGTGGAGACAA+TGG + chr8_4:41950206-41950225 Msa1326830:CDS 40.0%
AATCCGCTTCTTATCGTCTT+TGG - chr8_4:41950274-41950293 None:intergenic 40.0%
!! GGCATGAAGTTTTTGGAAAG+TGG + chr8_4:41950367-41950386 Msa1326830:CDS 40.0%
GCATCACTGAAGAACTCAAT+TGG - chr8_4:41950428-41950447 None:intergenic 40.0%
AGAAGATTCCTCACTCGGTT+CGG - chr8_4:41947673-41947692 None:intergenic 45.0%
!! GTTAGAAACTGGATTGGTGG+TGG - chr8_4:41947889-41947908 None:intergenic 45.0%
! TGTCTGTCTGTAGCTTTGCT+TGG - chr8_4:41947921-41947940 None:intergenic 45.0%
AAGCAAAGCTACAGACAGAC+AGG + chr8_4:41947920-41947939 Msa1326830:CDS 45.0%
ACAGAACTGGTTGAGTCGTT+AGG - chr8_4:41948836-41948855 None:intergenic 45.0%
AACGACTCAACCAGTTCTGT+TGG + chr8_4:41948836-41948855 Msa1326830:intron 45.0%
ACGACTCAACCAGTTCTGTT+GGG + chr8_4:41948837-41948856 Msa1326830:intron 45.0%
AAACCGAGAGCAGCTGAATT+TGG - chr8_4:41948965-41948984 None:intergenic 45.0%
TTTCTATCACCAACCTGCTC+AGG + chr8_4:41948982-41949001 Msa1326830:CDS 45.0%
TTCTATCACCAACCTGCTCA+GGG + chr8_4:41948983-41949002 Msa1326830:CDS 45.0%
CAAACCAATCTATGTGCTGG+TGG + chr8_4:41949016-41949035 Msa1326830:CDS 45.0%
CGAAAGAAAGATCAGTTCCC+TGG - chr8_4:41949051-41949070 None:intergenic 45.0%
GATGATACACTGGCATAGGA+AGG - chr8_4:41949145-41949164 None:intergenic 45.0%
TGGCATGATTGAGACTCTTG+TGG - chr8_4:41949187-41949206 None:intergenic 45.0%
!! ATGATTGGTGGTGATCTGAG+TGG - chr8_4:41949207-41949226 None:intergenic 45.0%
! AGGTGTTGTCAGATGATTGG+TGG - chr8_4:41949219-41949238 None:intergenic 45.0%
ACACTGGACATACCGCTTAA+CGG - chr8_4:41949250-41949269 None:intergenic 45.0%
!! GTATGTCCAGTGTTGACGAT+GGG + chr8_4:41949257-41949276 Msa1326830:CDS 45.0%
ATCATTCCCATCGTCAACAC+TGG - chr8_4:41949266-41949285 None:intergenic 45.0%
TGTGCACAAGCTGTCTATGA+TGG + chr8_4:41949469-41949488 Msa1326830:CDS 45.0%
GGTGATATTCCAACAGCAAG+AGG + chr8_4:41949490-41949509 Msa1326830:CDS 45.0%
ATATTCCAACAGCAAGAGGC+TGG + chr8_4:41949494-41949513 Msa1326830:CDS 45.0%
CGACTTGTAGATCAAGCTTC+CGG - chr8_4:41949620-41949639 None:intergenic 45.0%
! GTCATTAGCTCTTTGCTCGT+TGG - chr8_4:41949658-41949677 None:intergenic 45.0%
AAATCGCACAGGGAACACAA+TGG + chr8_4:41949794-41949813 Msa1326830:CDS 45.0%
TCGAATAAAAGGTGGTCCAC+CGG - chr8_4:41949851-41949870 None:intergenic 45.0%
CGGCATATGGTGCAAACTAA+AGG - chr8_4:41950067-41950086 None:intergenic 45.0%
TTACACTCTCATCCGGCATA+TGG - chr8_4:41950080-41950099 None:intergenic 45.0%
GGTTAAGAGTTTGTCGCCAA+AGG + chr8_4:41950134-41950153 Msa1326830:CDS 45.0%
TATCATGTTGACGATGTCCC+GGG - chr8_4:41950319-41950338 None:intergenic 45.0%
CTATCATGTTGACGATGTCC+CGG - chr8_4:41950320-41950339 None:intergenic 45.0%
GTCAACATGATAGCCTGTGA+GGG + chr8_4:41950327-41950346 Msa1326830:CDS 45.0%
!! GTGGAGAGGCATGAAGTTTT+TGG + chr8_4:41950360-41950379 Msa1326830:CDS 45.0%
GCATTGTCTAAATCCTGCCA+TGG - chr8_4:41950406-41950425 None:intergenic 45.0%
AGAGATGGTGCTCTCTATCT+TGG + chr8_4:41950501-41950520 Msa1326830:CDS 45.0%
ATGGTGCTCTCTATCTTGGT+TGG + chr8_4:41950505-41950524 Msa1326830:CDS 45.0%
TGGTTGGATGAAGAGAGCTA+TGG + chr8_4:41950521-41950540 Msa1326830:CDS 45.0%
ACATGGTTGGCACTGCATGA+CGG - chr8_4:41947267-41947286 None:intergenic 50.0%
TGTCAATTAAACCGGGACGG+AGG + chr8_4:41947541-41947560 Msa1326830:intron 50.0%
GTCAATTAAACCGGGACGGA+GGG + chr8_4:41947542-41947561 Msa1326830:intron 50.0%
ACTCGGCCGGAAAAGGAAAT+CGG - chr8_4:41948905-41948924 None:intergenic 50.0%
AGGCCAAATTCAGCTGCTCT+CGG + chr8_4:41948959-41948978 Msa1326830:CDS 50.0%
TGTAGGTAAATGCCCTGAGC+AGG - chr8_4:41948998-41949017 None:intergenic 50.0%
GCTACCACCAGCACATAGAT+TGG - chr8_4:41949023-41949042 None:intergenic 50.0%
ACCTATGGATCCCCGTTAAG+CGG + chr8_4:41949235-41949254 Msa1326830:CDS 50.0%
ACTGGACATACCGCTTAACG+GGG - chr8_4:41949248-41949267 None:intergenic 50.0%
CACTGGACATACCGCTTAAC+GGG - chr8_4:41949249-41949268 None:intergenic 50.0%
!! GGTATGTCCAGTGTTGACGA+TGG + chr8_4:41949256-41949275 Msa1326830:CDS 50.0%
!! GTGAAGATGGTATCGGTTGC+TGG + chr8_4:41949532-41949551 Msa1326830:CDS 50.0%
TTGCTGGAGATCCAATCCAG+AGG + chr8_4:41949548-41949567 Msa1326830:CDS 50.0%
! TTATTGAAGCGCTTGCACGC+AGG + chr8_4:41949824-41949843 Msa1326830:CDS 50.0%
CGAATAAAAGGTGGTCCACC+GGG - chr8_4:41949850-41949869 None:intergenic 50.0%
CAATCATTTCATGCACGCGG+TGG + chr8_4:41949892-41949911 Msa1326830:CDS 50.0%
GGTGGAGGACTTCAGATTGT+AGG + chr8_4:41949910-41949929 Msa1326830:CDS 50.0%
TCAAACTTTGCCAGGTCTCG+TGG + chr8_4:41949943-41949962 Msa1326830:CDS 50.0%
AAACAGAACTCCACGAGACC+TGG - chr8_4:41949956-41949975 None:intergenic 50.0%
GCGCCAAAGACGATAAGAAG+CGG + chr8_4:41950268-41950287 Msa1326830:CDS 50.0%
CGTCAACATGATAGCCTGTG+AGG + chr8_4:41950326-41950345 Msa1326830:CDS 50.0%
! GTGGAGGTCGAGATTTTCCA+TGG + chr8_4:41950386-41950405 Msa1326830:CDS 50.0%
! AGGTCGAGATTTTCCATGGC+AGG + chr8_4:41950390-41950409 Msa1326830:CDS 50.0%
CTAACACGTCCAAGCAGAAG+AGG - chr8_4:41950550-41950569 None:intergenic 50.0%
CAACTCAACCGAACCGAGTG+AGG + chr8_4:41947662-41947681 Msa1326830:intron 55.0%
GGTAAATGCCCTGAGCAGGT+TGG - chr8_4:41948994-41949013 None:intergenic 55.0%
!! GGTGAAGCGTTGCTGCTTGT+AGG - chr8_4:41949077-41949096 None:intergenic 55.0%
ACCGCTTAACGGGGATCCAT+AGG - chr8_4:41949239-41949258 None:intergenic 55.0%
GAATTCATTGCTGGGGCCTG+AGG + chr8_4:41949312-41949331 Msa1326830:CDS 55.0%
TGACAGTTACGGCAGCTGCA+TGG + chr8_4:41949348-41949367 Msa1326830:CDS 55.0%
!! TGCATGGAGACTAGCCTCCA+CGG + chr8_4:41949364-41949383 Msa1326830:CDS 55.0%
TAGCTGATGGAGATGCTCCG+TGG - chr8_4:41949384-41949403 None:intergenic 55.0%
CAGCTTGTGCACACAGCAAG+AGG - chr8_4:41949462-41949481 None:intergenic 55.0%
TCCAACAGCAAGAGGCTGGA+TGG + chr8_4:41949498-41949517 Msa1326830:CDS 55.0%
! TCCATCCAGCCTCTTGCTGT+TGG - chr8_4:41949502-41949521 None:intergenic 55.0%
TCATTTCATGCACGCGGTGG+AGG + chr8_4:41949895-41949914 Msa1326830:CDS 55.0%
TTCCACAGCGCTGCAATGTC+TGG + chr8_4:41949979-41949998 Msa1326830:CDS 55.0%
AACCAGACATTGCAGCGCTG+TGG - chr8_4:41949984-41950003 None:intergenic 55.0%
TAGTGCAGAGCAGAACTGCG+TGG + chr8_4:41950293-41950312 Msa1326830:CDS 55.0%
TAGCCTGTGAGGGAGTTGAG+AGG + chr8_4:41950337-41950356 Msa1326830:CDS 55.0%
AGCCTGTGAGGGAGTTGAGA+GGG + chr8_4:41950338-41950357 Msa1326830:CDS 55.0%
CTATGGCCACCTCTTCTGCT+TGG + chr8_4:41950538-41950557 Msa1326830:CDS 55.0%
ACACGTCCAAGCAGAAGAGG+TGG - chr8_4:41950547-41950566 None:intergenic 55.0%
GTAGTGTACTCCCTCCGTCC+CGG - chr8_4:41947555-41947574 None:intergenic 60.0%
CAAGCAGCAACGCTTCACCC+TGG + chr8_4:41949078-41949097 Msa1326830:CDS 60.0%
CTGATGGAGATGCTCCGTGG+AGG - chr8_4:41949381-41949400 None:intergenic 60.0%
TTTACGGCGAGGGCTTCTCC+AGG - chr8_4:41950039-41950058 None:intergenic 60.0%
CACCCTCTCAACTCCCTCAC+AGG - chr8_4:41950343-41950362 None:intergenic 60.0%
CTGTGAGGGAGTTGAGAGGG+TGG + chr8_4:41950341-41950360 Msa1326830:CDS 60.0%
AGGGAGTTGAGAGGGTGGAG+AGG + chr8_4:41950346-41950365 Msa1326830:CDS 60.0%
TGCTGGTGGTAGCAGCAGCC+AGG + chr8_4:41949030-41949049 Msa1326830:CDS 65.0%
GCTGGTGGTAGCAGCAGCCA+GGG + chr8_4:41949031-41949050 Msa1326830:CDS 65.0%
CAGAGCAGAACTGCGTGGCC+CGG + chr8_4:41950298-41950317 Msa1326830:CDS 65.0%
AGAGCAGAACTGCGTGGCCC+GGG + chr8_4:41950299-41950318 Msa1326830:CDS 65.0%
! GAAGCGCTTGCACGCAGGCC+CGG + chr8_4:41949829-41949848 Msa1326830:CDS 70.0%
! GCGCTTGCACGCAGGCCCGG+TGG + chr8_4:41949832-41949851 Msa1326830:CDS 80.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 41946977 41950569 41946977 ID=Msa1326830;Name=Msa1326830
chr8_4 mRNA 41946977 41950569 41946977 ID=Msa1326830-mRNA-1;Parent=Msa1326830;Name=Msa1326830-mRNA-1;_AED=0.12;_eAED=0.13;_QI=0|0|0|0.5|1|1|4|0|561
chr8_4 exon 41946977 41946980 41946977 ID=Msa1326830-mRNA-1:exon:12210;Parent=Msa1326830-mRNA-1
chr8_4 exon 41947880 41947941 41947880 ID=Msa1326830-mRNA-1:exon:12211;Parent=Msa1326830-mRNA-1
chr8_4 exon 41948252 41948257 41948252 ID=Msa1326830-mRNA-1:exon:12212;Parent=Msa1326830-mRNA-1
chr8_4 exon 41948956 41950569 41948956 ID=Msa1326830-mRNA-1:exon:12213;Parent=Msa1326830-mRNA-1
chr8_4 CDS 41946977 41946980 41946977 ID=Msa1326830-mRNA-1:cds;Parent=Msa1326830-mRNA-1
chr8_4 CDS 41947880 41947941 41947880 ID=Msa1326830-mRNA-1:cds;Parent=Msa1326830-mRNA-1
chr8_4 CDS 41948252 41948257 41948252 ID=Msa1326830-mRNA-1:cds;Parent=Msa1326830-mRNA-1
chr8_4 CDS 41948956 41950569 41948956 ID=Msa1326830-mRNA-1:cds;Parent=Msa1326830-mRNA-1
Gene Sequence

>Msa1326830

ATGGAAGCAACCACCACCAATCCAGTTTCTAACTCAAACACACCAAGCAAAGCTACAGACAGACAGAGAATACAAAGGCCAAATTCAGCTGCTCTCGGTTTCTATCACCAACCTGCTCAGGGCATTTACCTACAAACCAATCTATGTGCTGGTGGTAGCAGCAGCCAGGGAACTGATCTTTCTTTCGAAACCTACAAGCAGCAACGCTTCACCCTGGAATCATACCCAGAAATCACCGGTTTCATAGACTGTGATTCTCCTTCCTATGCCAGTGTATCATCTAACAAGACTCCATTTTCTCCACAAGAGTCTCAATCATGCCACTCAGATCACCACCAATCATCTGACAACACCTATGGATCCCCGTTAAGCGGTATGTCCAGTGTTGACGATGGGAATGATTTGAAGCACAAACTTAGAGAAATAGAGAATTCATTGCTGGGGCCTGAGGATTTTGATATTGTTGACAGTTACGGCAGCTGCATGGAGACTAGCCTCCACGGAGCATCTCCATCAGCTAAATATAATTGGGATCTGATTGCCGAAAACATTTCAAAGCTTGACATGAAGGAAGTCCTCTTGCTGTGTGCACAAGCTGTCTATGATGGTGATATTCCAACAGCAAGAGGCTGGATGGATAATGTATTGGTGAAGATGGTATCGGTTGCTGGAGATCCAATCCAGAGGTTAAGTGCTTACTTGTTGGAAGGGCTTAGAGCAAGATTAGAATCGTCCGGAAGCTTGATCTACAAGTCGTTGAAATGTGAACAACCAACGAGCAAAGAGCTAATGACTTACATGCACATGCTGTATCAGATTTGTCCATATTTTAAGTTTGCATACATATCTGCAAATGCTGTTATTTTTGAAGCAATGGCAAATGAATCCAGGATTCATATAATTGATTTTCAAATCGCACAGGGAACACAATGGCAAATGCTTATTGAAGCGCTTGCACGCAGGCCCGGTGGACCACCTTTTATTCGAATAACAGGGGTCGATGATTCACAATCATTTCATGCACGCGGTGGAGGACTTCAGATTGTAGGAGAACGACTTTCAAACTTTGCCAGGTCTCGTGGAGTTCTGTTTGAGTTCCACAGCGCTGCAATGTCTGGTTGCGAAGTTCAACGAGAAAATCTTAGAGTTAGTCCTGGAGAAGCCCTCGCCGTAAATTTTCCCTTTAGTTTGCACCATATGCCGGATGAGAGTGTAAGCATAGAGAATCATAGAGACAGATTGTTGAGATTGGTTAAGAGTTTGTCGCCAAAGGTTGTTACCCTTGTTGAGCAAGAATCCAACACCAATACTTCTCCTTTCTTTCAAAGGTTTGTGGAGACAATGGATTTTTACACGGCTATGTTTGAATCAATAGATGTTGCTTGCGCCAAAGACGATAAGAAGCGGATTAGTGCAGAGCAGAACTGCGTGGCCCGGGACATCGTCAACATGATAGCCTGTGAGGGAGTTGAGAGGGTGGAGAGGCATGAAGTTTTTGGAAAGTGGAGGTCGAGATTTTCCATGGCAGGATTTAGACAATGCCAATTGAGTTCTTCAGTGATGCATTCTGTCCAAAATATGTTGAAGGACTTCCATCAAAATTATAGGCTTGAACATAGAGATGGTGCTCTCTATCTTGGTTGGATGAAGAGAGCTATGGCCACCTCTTCTGCTTGGACGTGTTAG
Protein sequence

>Msa1326830

MEATTTNPVSNSNTPSKATDRQRIQRPNSAALGFYHQPAQGIYLQTNLCAGGSSSQGTDLSFETYKQQRFTLESYPEITGFIDCDSPSYASVSSNKTPFSPQESQSCHSDHHQSSDNTYGSPLSGMSSVDDGNDLKHKLREIENSLLGPEDFDIVDSYGSCMETSLHGASPSAKYNWDLIAENISKLDMKEVLLLCAQAVYDGDIPTARGWMDNVLVKMVSVAGDPIQRLSAYLLEGLRARLESSGSLIYKSLKCEQPTSKELMTYMHMLYQICPYFKFAYISANAVIFEAMANESRIHIIDFQIAQGTQWQMLIEALARRPGGPPFIRITGVDDSQSFHARGGGLQIVGERLSNFARSRGVLFEFHSAAMSGCEVQRENLRVSPGEALAVNFPFSLHHMPDESVSIENHRDRLLRLVKSLSPKVVTLVEQESNTNTSPFFQRFVETMDFYTAMFESIDVACAKDDKKRISAEQNCVARDIVNMIACEGVERVERHEVFGKWRSRFSMAGFRQCQLSSSVMHSVQNMLKDFHQNYRLEHRDGALYLGWMKRAMATSSAWTC*