AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1327200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1327200 MtrunA17_Chr6g0456201 95.588 1020 44 1 1 1019 1 1020 0.0 1963
Msa1327200 MtrunA17_Chr7g0260401 65.166 1022 312 11 28 1013 34 1047 0.0 1255
Msa1327200 MtrunA17_Chr8g0340261 89.142 571 44 2 449 1018 1 554 0.0 1036
Msa1327200 MtrunA17_Chr2g0298871 56.681 958 393 9 66 1010 126 1074 0.0 1031
Msa1327200 MtrunA17_Chr4g0002331 56.140 969 395 12 54 1010 136 1086 0.0 1018
Msa1327200 MtrunA17_Chr1g0173111 45.858 1014 496 17 6 1005 37 1011 0.0 852
Msa1327200 MtrunA17_Chr8g0382071 47.534 953 457 17 70 1008 94 1017 0.0 839
Msa1327200 MtrunA17_Chr8g0340271 90.414 459 36 3 1 459 1 451 0.0 818
Msa1327200 MtrunA17_Chr4g0054021 47.808 958 465 13 57 1005 6 937 0.0 804
Msa1327200 MtrunA17_Chr3g0134771 47.441 938 454 12 80 1005 106 1016 0.0 793
Msa1327200 MtrunA17_Chr5g0401211 47.780 946 458 13 70 1005 95 1014 0.0 791
Msa1327200 MtrunA17_Chr4g0020551 46.400 875 426 14 70 929 27 873 0.0 749
Msa1327200 MtrunA17_Chr8g0355421 40.821 975 527 17 45 1005 67 1005 0.0 685
Msa1327200 MtrunA17_Chr5g0447521 33.982 874 520 15 153 1003 165 1004 1.21e-143 456
Msa1327200 MtrunA17_Chr4g0005551 31.224 948 584 17 80 1005 66 967 9.07e-137 437
Msa1327200 MtrunA17_Chr2g0334231 50.485 412 191 3 41 452 106 504 1.30e-123 387
Msa1327200 MtrunA17_Chr7g0263361 32.683 872 464 26 102 886 38 873 7.43e-108 360
Msa1327200 MtrunA17_Chr8g0346461 31.307 872 479 25 107 886 25 868 3.35e-95 325
Msa1327200 MtrunA17_Chr3g0134951 29.673 856 508 22 96 886 3 829 1.44e-86 300
Msa1327200 MtrunA17_Chr2g0334221 52.174 299 135 6 470 765 43 336 1.09e-84 277
Msa1327200 MtrunA17_Chr2g0334201 55.952 168 69 1 847 1009 1 168 4.67e-61 206
Msa1327200 MtrunA17_Chr8g0381711 25.835 778 469 25 155 901 69 769 5.27e-48 185
Msa1327200 MtrunA17_Chr4g0065481 23.522 761 463 27 72 817 1 657 4.22e-45 176
Msa1327200 MtrunA17_Chr4g0072131 24.209 727 456 22 95 817 24 659 7.51e-45 176
Msa1327200 MtrunA17_Chr2g0298031 24.126 601 374 11 220 817 141 662 2.92e-44 174
Msa1327200 MtrunA17_Chr6g0452721 23.974 609 364 18 220 817 138 658 2.01e-42 168
Msa1327200 MtrunA17_Chr3g0138931 23.752 741 468 21 81 817 9 656 4.28e-42 167
Msa1327200 MtrunA17_Chr1g0148031 24.621 593 352 18 234 817 150 656 7.43e-42 166
Msa1327200 MtrunA17_Chr2g0304941 23.328 613 363 16 220 817 137 657 8.30e-42 166
Msa1327200 MtrunA17_Chr2g0333721 24.212 603 370 15 220 817 134 654 5.42e-41 163
Msa1327200 MtrunA17_Chr4g0059961 24.547 607 363 18 220 817 140 660 5.70e-41 163
Msa1327200 MtrunA17_Chr8g0365021 23.597 606 370 16 220 817 206 726 1.20e-40 162
Msa1327200 MtrunA17_Chr1g0147981 23.501 617 357 19 220 817 153 673 1.81e-39 159
Msa1327200 MtrunA17_Chr8g0335291 23.214 616 359 17 220 817 144 663 2.74e-37 152
Msa1327200 MtrunA17_Chr5g0421231 80.000 80 16 0 522 601 279 358 8.43e-32 134
Msa1327200 MtrunA17_Chr7g0276551 24.946 465 291 11 387 848 42 451 1.02e-30 130
Msa1327200 MtrunA17_Chr3g0131151 22.604 814 481 25 52 780 94 843 3.61e-22 103
Msa1327200 MtrunA17_Chr2g0324791 21.258 795 457 22 213 863 98 867 9.11e-20 95.5
Msa1327200 MtrunA17_Chr8g0374391 29.032 186 100 3 637 822 32 185 7.03e-19 87.4
Msa1327200 MtrunA17_Chr8g0374331 29.032 186 100 3 637 822 39 192 7.79e-19 87.4
Msa1327200 MtrunA17_Chr2g0297051 29.353 201 109 3 622 822 804 971 1.70e-18 91.7
Msa1327200 MtrunA17_Chr4g0052521 28.141 199 110 3 622 820 772 937 4.20e-17 87.0
Msa1327200 MtrunA17_Chr5g0397621 30.000 190 97 5 613 799 664 820 5.10e-17 86.7
Msa1327200 MtrunA17_Chr8g0339181 29.703 202 103 4 640 840 759 922 3.47e-16 84.0
Msa1327200 MtrunA17_Chr4g0062251 22.881 472 266 19 502 890 555 1011 2.24e-15 81.3
Msa1327200 MtrunA17_Chr5g0415271 21.244 659 357 23 204 718 105 745 6.67e-13 73.2
Msa1327200 MtrunA17_Chr2g0297871 29.193 161 84 3 639 799 356 486 4.24e-12 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1327200 AT3G22910.1 67.321 1019 301 13 12 1018 17 1015 0.0 1295
Msa1327200 AT3G63380.1 65.675 1008 309 10 27 1014 25 1015 0.0 1270
Msa1327200 AT4G29900.1 53.956 1049 438 12 6 1018 23 1062 0.0 1069
Msa1327200 AT4G29900.2 53.956 1049 438 12 6 1018 23 1062 0.0 1069
Msa1327200 AT5G57110.1 56.341 962 400 8 66 1018 110 1060 0.0 1055
Msa1327200 AT5G57110.3 56.341 962 400 8 66 1018 110 1060 0.0 1055
Msa1327200 AT5G57110.2 56.341 962 400 8 66 1018 110 1060 0.0 1055
Msa1327200 AT3G21180.1 52.743 1039 431 15 19 1010 44 1069 0.0 1011
Msa1327200 AT3G57330.1 46.937 963 474 12 57 1012 80 1012 0.0 861
Msa1327200 AT3G57330.2 46.937 963 474 12 57 1012 25 957 0.0 861
Msa1327200 AT2G41560.1 44.543 1017 516 15 6 1012 37 1015 0.0 841
Msa1327200 AT2G41560.2 46.448 943 465 12 80 1012 1 913 0.0 828
Msa1327200 AT2G41560.3 46.448 943 465 12 80 1012 1 913 0.0 828
Msa1327200 AT2G41560.4 46.448 943 465 12 80 1012 1 913 0.0 828
Msa1327200 AT1G27770.4 46.835 948 466 13 70 1005 96 1017 0.0 796
Msa1327200 AT1G27770.1 46.835 948 466 13 70 1005 96 1017 0.0 796
Msa1327200 AT1G27770.3 46.835 948 466 13 70 1005 33 954 0.0 796
Msa1327200 AT4G37640.1 46.930 961 474 15 56 1005 79 1014 0.0 778
Msa1327200 AT2G22950.1 46.646 954 459 12 70 1005 94 1015 0.0 769
Msa1327200 AT5G53010.1 36.632 950 520 19 70 993 140 1033 2.35e-174 537
Msa1327200 AT5G53010.2 37.054 645 387 10 70 713 140 766 2.22e-122 392
Msa1327200 AT1G27770.2 41.324 559 304 9 70 621 96 637 2.68e-116 381
Msa1327200 AT1G27770.2 56.634 309 129 2 702 1005 635 943 1.21e-106 355
Msa1327200 AT1G07670.2 32.865 855 476 25 107 886 37 868 5.76e-108 361
Msa1327200 AT1G07670.1 32.865 855 476 25 107 886 37 868 5.76e-108 361
Msa1327200 AT1G07810.1 33.099 855 468 26 107 886 43 868 4.99e-107 358
Msa1327200 AT4G00900.2 32.907 860 465 27 95 869 14 846 7.45e-101 341
Msa1327200 AT4G00900.1 32.907 860 465 27 95 869 14 846 7.45e-101 341
Msa1327200 AT1G10130.1 30.851 470 284 9 565 1003 511 970 2.22e-54 206
Msa1327200 AT1G10130.1 30.458 371 211 9 108 456 16 361 9.07e-30 128
Msa1327200 AT2G24520.3 24.320 588 360 13 234 817 130 636 2.10e-43 171
Msa1327200 AT2G24520.1 24.320 588 360 13 234 817 130 636 2.10e-43 171
Msa1327200 AT2G24520.2 24.320 588 360 13 234 817 193 699 2.82e-43 171
Msa1327200 AT1G80660.3 24.302 609 362 18 220 817 139 659 9.39e-43 169
Msa1327200 AT1G80660.2 24.466 609 361 19 220 817 10 530 9.67e-43 169
Msa1327200 AT1G80660.4 24.302 609 362 18 220 817 139 659 1.04e-42 169
Msa1327200 AT1G80660.1 24.302 609 362 18 220 817 139 659 1.13e-42 169
Msa1327200 AT3G42640.1 23.169 751 464 25 79 817 8 657 5.56e-42 167
Msa1327200 AT2G07560.1 22.842 753 463 25 79 817 8 656 1.07e-41 166
Msa1327200 AT3G60330.4 23.888 607 362 18 221 817 138 654 1.24e-41 166
Msa1327200 AT3G60330.3 23.888 607 362 18 221 817 138 654 1.24e-41 166
Msa1327200 AT1G17260.1 25.253 594 346 16 234 817 156 661 1.35e-41 166
Msa1327200 AT3G60330.2 23.723 607 363 18 221 817 138 654 2.34e-41 165
Msa1327200 AT3G60330.1 23.723 607 363 18 221 817 138 654 2.34e-41 165
Msa1327200 AT3G47950.1 23.770 610 364 18 220 817 142 662 4.77e-41 164
Msa1327200 AT3G47950.2 23.770 610 364 18 220 817 142 662 4.77e-41 164
Msa1327200 AT5G62670.1 23.684 608 367 17 220 817 138 658 2.37e-40 162
Msa1327200 AT4G30190.1 24.086 602 372 15 220 817 134 654 1.49e-38 156
Msa1327200 AT2G18960.3 23.920 602 373 15 220 817 70 590 4.70e-38 154
Msa1327200 AT2G18960.2 23.920 602 373 15 220 817 70 590 4.70e-38 154
Msa1327200 AT2G18960.1 23.920 602 373 15 220 817 134 654 7.17e-38 154
Msa1327200 AT5G57350.4 23.404 611 365 16 220 817 135 655 7.36e-38 154
Msa1327200 AT5G57350.2 23.404 611 365 16 220 817 135 655 7.36e-38 154
Msa1327200 AT5G57350.1 23.404 611 365 16 220 817 135 655 7.36e-38 154
Msa1327200 AT4G30190.2 23.028 634 372 18 220 817 134 687 5.20e-35 145
Msa1327200 AT2G24520.4 23.570 437 272 10 387 817 13 393 6.61e-29 124
Msa1327200 AT5G57350.3 24.208 442 263 12 387 817 13 393 4.58e-27 118
Msa1327200 AT5G23630.1 21.688 770 462 21 74 767 125 829 3.52e-23 107
Msa1327200 AT1G63440.1 30.435 207 108 6 619 822 764 937 2.92e-20 97.4
Msa1327200 AT5G44790.1 31.188 202 106 4 621 822 780 948 8.85e-18 89.7
Msa1327200 AT4G11730.1 22.420 281 187 7 180 456 92 345 5.26e-15 80.5
Msa1327200 AT4G30110.1 27.143 210 113 4 639 848 510 679 1.11e-13 76.3
Msa1327200 AT4G30110.2 27.143 210 113 4 639 848 510 679 1.11e-13 76.3
Msa1327200 AT2G19110.2 26.809 235 130 6 640 874 521 713 2.95e-12 71.6
Msa1327200 AT2G19110.3 26.809 235 130 6 640 874 521 713 2.95e-12 71.6
Msa1327200 AT2G19110.1 26.809 235 130 6 640 874 521 713 2.95e-12 71.6

Find 157 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGATGATACTAATGATTT+TGG 0.155042 8_4:+43736357 Msa1327200:CDS
TAACAGCAGTACAATTATTA+TGG 0.188641 8_4:+43738782 Msa1327200:CDS
TCATGAAAATCTATTGTTCT+AGG 0.205768 8_4:+43736124 Msa1327200:CDS
TGAGTTTAATGCAAGGAAAT+TGG 0.225227 8_4:+43739065 Msa1327200:CDS
ATTATGGGTCAATTTGATTA+TGG 0.242182 8_4:+43738798 Msa1327200:CDS
AATAATTGTACTGCTGTTAA+TGG 0.248421 8_4:-43738778 None:intergenic
GTTAGGGCTTGTCGGTATTA+AGG 0.265803 8_4:+43738076 Msa1327200:CDS
GGTGCTGTTTCTTGGCCTAT+TGG 0.270147 8_4:+43739246 Msa1327200:CDS
GTTCAATGTTTAAAACAAAA+AGG 0.271876 8_4:+43738481 Msa1327200:CDS
AATACTATCTTGGGCTGTTT+TGG 0.272622 8_4:+43737695 Msa1327200:CDS
ACTCTCTGCATGTGAGACTA+TGG 0.284040 8_4:+43737301 Msa1327200:CDS
GACAATGATTTAACATTGTT+AGG 0.284588 8_4:+43738059 Msa1327200:CDS
TCCATTTGACAAACTTCTAA+TGG 0.296674 8_4:+43738459 Msa1327200:CDS
AAAGCCTACCAAGGAATTAA+TGG 0.302816 8_4:+43738852 Msa1327200:CDS
TTTGGAATTGAAAATGGATA+TGG 0.303373 8_4:+43737713 Msa1327200:CDS
TCCCATTGAAAGACCGATAT+CGG 0.305491 8_4:-43738560 None:intergenic
GAGTTAACAAAATTACTATT+TGG 0.307036 8_4:-43738951 None:intergenic
CTTAAAACCAACATTGAGTT+TGG 0.307048 8_4:+43736327 Msa1327200:CDS
CAATGACATTCAAATTGATT+TGG 0.308945 8_4:+43736662 Msa1327200:CDS
GTTTCATCTGTGTCCTCATT+AGG 0.309923 8_4:-43738349 None:intergenic
AGATGAAGGTGACGAAGTTT+TGG 0.311024 8_4:+43737508 Msa1327200:CDS
TCGAAGTGGTCGTCGGTTAA+AGG 0.314231 8_4:+43736686 Msa1327200:CDS
TGGATTATTTGTATTGGTTT+TGG 0.330074 8_4:+43739225 Msa1327200:CDS
TTGATCAATTGAAGAACATA+TGG 0.343534 8_4:-43737576 None:intergenic
CACATAATGTTAGTTATAAG+AGG 0.361954 8_4:-43738901 None:intergenic
CTGGTACAGAAAGGTTGAAT+TGG 0.367852 8_4:+43739196 Msa1327200:CDS
GGCCATGTAGTTGCTGTAAC+AGG 0.373182 8_4:+43738502 Msa1327200:CDS
AACAGCAGTACAATTATTAT+GGG 0.378279 8_4:+43738783 Msa1327200:CDS
GCTACACTTTCACTTGGCTT+TGG 0.378331 8_4:+43736504 Msa1327200:CDS
AAAGGTTGAATTGGAGAGAA+TGG 0.383579 8_4:+43739205 Msa1327200:CDS
GCTGATGGATTGTTTGTAGA+TGG 0.387434 8_4:+43736771 Msa1327200:CDS
AATTTGATTATGGATACATT+AGG 0.389466 8_4:+43738808 Msa1327200:CDS
GTGAATGTCAAAATGATTAC+AGG 0.392744 8_4:+43738152 Msa1327200:CDS
GATGTGATGAATGCAATTAT+TGG 0.400166 8_4:+43737155 Msa1327200:CDS
GATGTTGTTTGTTTGAAGAT+TGG 0.403237 8_4:+43736735 Msa1327200:CDS
TCAAGATACTATGATGGTTA+TGG 0.406417 8_4:+43737882 Msa1327200:CDS
GCAATTAGGAAACAAGTGTT+TGG 0.412156 8_4:+43736387 Msa1327200:CDS
AGAGGCTTTGTTCTACCAAC+TGG 0.413768 8_4:-43738883 None:intergenic
ATCGATCGGAAAAGTTGGTT+TGG 0.416711 8_4:+43737025 Msa1327200:CDS
ACCATTAGAAGTTTGTCAAA+TGG 0.418812 8_4:-43738460 None:intergenic
CGGAAAAGTTGGTTTGGTTG+TGG 0.424627 8_4:+43737031 Msa1327200:CDS
AGAGAATGGATTATTTGTAT+TGG 0.428342 8_4:+43739219 Msa1327200:CDS
CAAAATCATGGTGGAGTTGA+AGG 0.433331 8_4:+43736291 Msa1327200:CDS
CATGAAAATCTATTGTTCTA+GGG 0.434432 8_4:+43736125 Msa1327200:CDS
TTATCCATTAATTCCTTGGT+AGG 0.435304 8_4:-43738856 None:intergenic
AGTGTCAATGTCACAGCTAA+TGG 0.439120 8_4:-43737230 None:intergenic
TATGAGAGTTTCTTGATCAA+TGG 0.439612 8_4:-43736235 None:intergenic
TGGTCTAGAGCCATTGGAAA+AGG 0.442604 8_4:+43737533 Msa1327200:CDS
ACAATGATTTAACATTGTTA+GGG 0.444141 8_4:+43738060 Msa1327200:CDS
ACTATTTGCACCGACAAAAC+AGG 0.445850 8_4:+43737335 Msa1327200:CDS
GTTCTTCAATTGATCAAAGA+AGG 0.446882 8_4:+43737582 Msa1327200:CDS
TCCACCGCTTTCTTCACCCC+TGG 0.447188 8_4:-43738110 None:intergenic
GGAGTTGCACTCAATACAAC+TGG 0.449947 8_4:+43737603 Msa1327200:CDS
TCAATATTTGATATTGTTGT+TGG 0.451599 8_4:+43736711 Msa1327200:CDS
CTATTGATTCGATATTTCAC+TGG 0.452263 8_4:+43737074 Msa1327200:CDS
CGAAAGCATTATTCAAGGTA+TGG 0.454195 8_4:+43737944 Msa1327200:CDS
CATCTGTGTCCTCATTAGGT+TGG 0.458267 8_4:-43738345 None:intergenic
AGCCGATATCGGTCTTTCAA+TGG 0.458474 8_4:+43738558 Msa1327200:CDS
GTTGATGAATCGAGCATGAC+AGG 0.461070 8_4:+43736807 Msa1327200:CDS
AAAATGCTTGTTACATCGGT+TGG 0.465518 8_4:+43736909 Msa1327200:CDS
TATGGATACATTAGGTGCAT+TGG 0.476382 8_4:+43738816 Msa1327200:CDS
TTGGCTTGGTCTAGAGCCAT+TGG 0.476465 8_4:+43737527 Msa1327200:CDS
GTTTGTGCTACACTTTCACT+TGG 0.479286 8_4:+43736498 Msa1327200:CDS
GGTCTTTCAATGGGAATTCA+AGG 0.480265 8_4:+43738568 Msa1327200:CDS
TTAACATTGTTAGGGCTTGT+CGG 0.483188 8_4:+43738068 Msa1327200:CDS
GCCGATATCGGTCTTTCAAT+GGG 0.484090 8_4:+43738559 Msa1327200:CDS
GGTGTTCACAAATCGAAATC+AGG 0.487786 8_4:+43737627 Msa1327200:CDS
AGGTGGTTTCTTATAAGTGT+TGG 0.488366 8_4:-43736412 None:intergenic
AAATCAACCACGGTGAAAGA+TGG 0.493237 8_4:-43736192 None:intergenic
ATTGACACACTTCAAAATCA+TGG 0.494685 8_4:+43736279 Msa1327200:CDS
AACTTCACAACAAAACCAAT+AGG 0.494710 8_4:-43739261 None:intergenic
TTCTTCACCCCTGGACGACA+TGG 0.495536 8_4:-43738101 None:intergenic
TCACAAATCGAAATCAGGTT+CGG 0.498715 8_4:+43737632 Msa1327200:CDS
ACAGATGAAACCGTGGTTGA+AGG 0.498794 8_4:+43738361 Msa1327200:CDS
ATCGTAGCCGCGGTTTCAGC+CGG 0.498836 8_4:+43738748 Msa1327200:CDS
GGGAATTATTGGTGTGACAT+TGG 0.499595 8_4:+43739134 Msa1327200:CDS
TTGTTAACTCTTCAATTCAA+AGG 0.499732 8_4:+43738964 Msa1327200:CDS
ATTAAGGATCCATGTCGTCC+AGG 0.505479 8_4:+43738092 Msa1327200:CDS
TTTGATACCAAACTCAATGT+TGG 0.505557 8_4:-43736334 None:intergenic
ACATCATCGATCGGAAAAGT+TGG 0.506780 8_4:+43737020 Msa1327200:CDS
AGGAAACACCTTTGCAAACA+AGG 0.506800 8_4:+43736985 Msa1327200:CDS
AAATTCGAAAGCATTATTCA+AGG 0.506879 8_4:+43737939 Msa1327200:CDS
CGGAATTCTCCAACCTAATG+AGG 0.515315 8_4:+43738336 Msa1327200:CDS
AGAAATGGTTTATCTGAGAC+AGG 0.517777 8_4:-43739288 None:intergenic
ATCAAAGAAGGTTGGTATGA+TGG 0.518871 8_4:+43736543 Msa1327200:CDS
TGCTGTTAATGGTACTTCAC+CGG 0.520634 8_4:-43738767 None:intergenic
GCATTGAAAGAAGCCGATAT+CGG 0.523732 8_4:+43738547 Msa1327200:CDS
AGAAGTTTGCTGGTACAGAA+AGG 0.529712 8_4:+43739187 Msa1327200:CDS
GTTGTTGTTGCAATTCCTGA+GGG 0.531565 8_4:+43737203 Msa1327200:CDS
CTCTTATAACTAACATTATG+TGG 0.531696 8_4:+43738902 Msa1327200:CDS
AAATTGTTCGCCTTCAACCA+CGG 0.532190 8_4:-43738371 None:intergenic
ATATCGGCTTCTTTCAATGC+CGG 0.532373 8_4:-43738544 None:intergenic
AAAGTGTAGCACAAACCAAA+AGG 0.534473 8_4:-43736491 None:intergenic
GCAGACGAAGAGCTTGGAGA+TGG 0.537228 8_4:+43738011 Msa1327200:CDS
AACTCAAAGAAGAAAAGAAG+TGG 0.537966 8_4:+43737780 Msa1327200:CDS
ATAACACACACAACAAAAGA+TGG 0.538699 8_4:+43736091 Msa1327200:CDS
TTGGAAAGGAGCAGCAGAGA+TGG 0.539480 8_4:+43737845 Msa1327200:CDS
AACTCTTCAATTCAAAGGTG+AGG 0.539506 8_4:+43738969 Msa1327200:CDS
CTCTCTGCATGTGAGACTAT+GGG 0.539612 8_4:+43737302 Msa1327200:CDS
TTAAGGATCCATGTCGTCCA+GGG 0.540918 8_4:+43738093 Msa1327200:CDS
ATTGGTGCTTCAAGTTGTGA+TGG 0.541508 8_4:+43739152 Msa1327200:CDS
GTAGTTGCTGTAACAGGAGA+TGG 0.541746 8_4:+43738508 Msa1327200:CDS
ACAGCTAATGGCAAACCCTC+AGG 0.545616 8_4:-43737218 None:intergenic
TTATTCAACCTTGTTTGCAA+AGG 0.545979 8_4:-43736993 None:intergenic
AAAGTCGCAGACGAAGAGCT+TGG 0.546151 8_4:+43738005 Msa1327200:CDS
CGGTTGGTATGAACACGACA+TGG 0.547674 8_4:+43736925 Msa1327200:CDS
CAAATTGATTTGGTTCGAAG+TGG 0.549306 8_4:+43736672 Msa1327200:CDS
ATTTGGTTCGAAGTGGTCGT+CGG 0.551230 8_4:+43736679 Msa1327200:CDS
GAAGATTGGTGATCAAGTAC+CGG 0.553519 8_4:+43736749 Msa1327200:CDS
AACATGGAATCAAAGAAGGT+TGG 0.557290 8_4:+43736535 Msa1327200:CDS
AAGCAACATGGAATCAAAGA+AGG 0.557387 8_4:+43736531 Msa1327200:CDS
CTCCTGTTACAGCAACTACA+TGG 0.559264 8_4:-43738504 None:intergenic
TGGAGACAATAACAAGATGA+TGG 0.560154 8_4:+43738031 Msa1327200:CDS
GGTGATCAAGTACCGGCTGA+TGG 0.561728 8_4:+43736756 Msa1327200:CDS
TTATTCAATGAAGAAAATGA+TGG 0.562067 8_4:+43737256 Msa1327200:CDS
GGTCTAGAGCCATTGGAAAA+GGG 0.568153 8_4:+43737534 Msa1327200:CDS
AAAACTATTGCACATTGGAA+AGG 0.568293 8_4:+43737831 Msa1327200:CDS
TGTTGTTGTTGCAATTCCTG+AGG 0.573357 8_4:+43737202 Msa1327200:CDS
GTGGAAGCTTGCCAACATGC+CGG 0.573699 8_4:+43738128 Msa1327200:CDS
ATTTCAATTAACAGTGAATG+TGG 0.574758 8_4:+43738708 Msa1327200:CDS
TATTGATTCGATATTTCACT+GGG 0.578618 8_4:+43737075 Msa1327200:CDS
AGATGGAACAAATGATGCAC+CGG 0.583689 8_4:+43738525 Msa1327200:CDS
ACTCACTTTGAATCAGATGA+AGG 0.584042 8_4:+43737494 Msa1327200:CDS
TACTTCACCGGCTGAAACCG+CGG 0.586833 8_4:-43738755 None:intergenic
ACAACAAAACTATTGCACAT+TGG 0.588164 8_4:+43737826 Msa1327200:CDS
TGACATTCACACCGGCATGT+TGG 0.590504 8_4:-43738139 None:intergenic
ATGTGTTCAAGATACTATGA+TGG 0.592425 8_4:+43737876 Msa1327200:CDS
TCGGGGACAAAAGTTGTCGA+TGG 0.594676 8_4:+43736879 Msa1327200:CDS
GGTTGGTATGAACACGACAT+GGG 0.607578 8_4:+43736926 Msa1327200:CDS
TCTACAAACAATCCATCAGC+CGG 0.619777 8_4:-43736768 None:intergenic
AATAAGCTAACATCATCGAT+CGG 0.622025 8_4:+43737011 Msa1327200:CDS
TGTTTAATGAGTTTAATGCA+AGG 0.630387 8_4:+43739058 Msa1327200:CDS
GGCTTTGGTATCAAGCAACA+TGG 0.632329 8_4:+43736519 Msa1327200:CDS
TGCAACTGAAAAGCCTACCA+AGG 0.652731 8_4:+43738843 Msa1327200:CDS
AAAGAAGGTTGGTATGATGG+TGG 0.658285 8_4:+43736546 Msa1327200:CDS
TGTTATCAATATCGTAGCCG+CGG 0.661804 8_4:+43738738 Msa1327200:CDS
TTATAACTAACATTATGTGG+AGG 0.663994 8_4:+43738905 Msa1327200:CDS
AGCGGAGTTAGAGAGTTCAA+CGG 0.664012 8_4:+43737113 Msa1327200:CDS
TCGTCCAGGGGTGAAGAAAG+CGG 0.665154 8_4:+43738106 Msa1327200:CDS
TAAGGATCCATGTCGTCCAG+GGG 0.675223 8_4:+43738094 Msa1327200:CDS
TCCAGGGGTGAAGAAAGCGG+TGG 0.675734 8_4:+43738109 Msa1327200:CDS
AAGCATTATTCAAGGTATGG+CGG 0.676719 8_4:+43737947 Msa1327200:CDS
AATGATGGCTGATCAAGCAA+TGG 0.680846 8_4:+43737271 Msa1327200:CDS
GTTGGTATGAACACGACATG+GGG 0.684579 8_4:+43736927 Msa1327200:CDS
GGGAACACAAAGAACGATAG+CGG 0.689697 8_4:+43737095 Msa1327200:CDS
TGATCAATGGTAAAAGAGTG+TGG 0.699257 8_4:-43736222 None:intergenic
ACCTACTCCATCTTTCACCG+TGG 0.705281 8_4:+43736185 Msa1327200:CDS
GTTGCACTCAATACAACTGG+AGG 0.710232 8_4:+43737606 Msa1327200:CDS
ACCACGGTGAAAGATGGAGT+AGG 0.718242 8_4:-43736186 None:intergenic
TGGTGGATTTAAATCAACCA+CGG 0.718660 8_4:-43736202 None:intergenic
AAAGCTATAGCATTTGAATG+CGG 0.727776 8_4:+43738316 Msa1327200:CDS
GACACACTTCAAAATCATGG+TGG 0.728470 8_4:+43736282 Msa1327200:CDS
TGAGGACACAGATGAAACCG+TGG 0.741833 8_4:+43738354 Msa1327200:CDS
AAATAACGATGAAAACAGCG+AGG 0.747712 8_4:-43736578 None:intergenic
ATCGAATCAATAGAACAACA+AGG 0.750277 8_4:-43737064 None:intergenic
TCAATGGTAAAAGAGTGTGG+TGG 0.763849 8_4:-43736219 None:intergenic
GATAAGCAATGACATCGACG+AGG 0.796368 8_4:+43736965 Msa1327200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGATATTTGATTTGTTAT+AGG + chr8_4:43737468-43737487 Msa1327200:intron 15.0%
!!! TATAGTAACAGTTTTAAATT+GGG + chr8_4:43738648-43738667 Msa1327200:CDS 15.0%
!!! AAATGAATGAAACTTTTAGA+AGG - chr8_4:43736435-43736454 None:intergenic 20.0%
!!! TGTAACAATTTTAATCCTTT+TGG + chr8_4:43736476-43736495 Msa1327200:CDS 20.0%
!! TCAATATTTGATATTGTTGT+TGG + chr8_4:43736711-43736730 Msa1327200:CDS 20.0%
!! TTATTCAATGAAGAAAATGA+TGG + chr8_4:43737256-43737275 Msa1327200:CDS 20.0%
!! TTAAGAAAAAAGTTGTAACA+AGG + chr8_4:43737435-43737454 Msa1327200:intron 20.0%
!! ACAATGATTTAACATTGTTA+GGG + chr8_4:43738060-43738079 Msa1327200:CDS 20.0%
!!! GTTCAATGTTTAAAACAAAA+AGG + chr8_4:43738481-43738500 Msa1327200:CDS 20.0%
!!! CTATAGTAACAGTTTTAAAT+TGG + chr8_4:43738647-43738666 Msa1327200:CDS 20.0%
!!! ATAGTAACAGTTTTAAATTG+GGG + chr8_4:43738649-43738668 Msa1327200:CDS 20.0%
!! AATTTGATTATGGATACATT+AGG + chr8_4:43738808-43738827 Msa1327200:CDS 20.0%
!!! TTTTTTATCCATTAATTCCT+TGG - chr8_4:43738863-43738882 None:intergenic 20.0%
!! GAGTTAACAAAATTACTATT+TGG - chr8_4:43738954-43738973 None:intergenic 20.0%
!! ATACTCAAAAAATCAAGAAA+TGG - chr8_4:43739306-43739325 None:intergenic 20.0%
! TCATGAAAATCTATTGTTCT+AGG + chr8_4:43736124-43736143 Msa1327200:CDS 25.0%
! CATGAAAATCTATTGTTCTA+GGG + chr8_4:43736125-43736144 Msa1327200:CDS 25.0%
!! AGTGATGATACTAATGATTT+TGG + chr8_4:43736357-43736376 Msa1327200:CDS 25.0%
!!! TTTTGTTGTTGAAGCTTTTA+AGG + chr8_4:43736452-43736471 Msa1327200:CDS 25.0%
! CAATGACATTCAAATTGATT+TGG + chr8_4:43736662-43736681 Msa1327200:CDS 25.0%
!!! TTTTCATCCTTTTTTGCTTT+CGG + chr8_4:43736860-43736879 Msa1327200:CDS 25.0%
!!! TATTGATTCGATATTTCACT+GGG + chr8_4:43737075-43737094 Msa1327200:CDS 25.0%
! TTGATCAATTGAAGAACATA+TGG - chr8_4:43737579-43737598 None:intergenic 25.0%
! AGAAAAAGCAATACTATCTT+GGG + chr8_4:43737686-43737705 Msa1327200:CDS 25.0%
!! TTTGGAATTGAAAATGGATA+TGG + chr8_4:43737713-43737732 Msa1327200:CDS 25.0%
!!! AAATTCGAAAGCATTATTCA+AGG + chr8_4:43737939-43737958 Msa1327200:CDS 25.0%
! GACAATGATTTAACATTGTT+AGG + chr8_4:43738059-43738078 Msa1327200:CDS 25.0%
! ATTTCAATTAACAGTGAATG+TGG + chr8_4:43738708-43738727 Msa1327200:CDS 25.0%
! AATAATTGTACTGCTGTTAA+TGG - chr8_4:43738781-43738800 None:intergenic 25.0%
! TAACAGCAGTACAATTATTA+TGG + chr8_4:43738782-43738801 Msa1327200:CDS 25.0%
! AACAGCAGTACAATTATTAT+GGG + chr8_4:43738783-43738802 Msa1327200:CDS 25.0%
! ATTATGGGTCAATTTGATTA+TGG + chr8_4:43738798-43738817 Msa1327200:CDS 25.0%
!! TTAATGGATAAAAAACCAGT+TGG + chr8_4:43738868-43738887 Msa1327200:CDS 25.0%
! CACATAATGTTAGTTATAAG+AGG - chr8_4:43738904-43738923 None:intergenic 25.0%
! CTCTTATAACTAACATTATG+TGG + chr8_4:43738902-43738921 Msa1327200:CDS 25.0%
! TTATAACTAACATTATGTGG+AGG + chr8_4:43738905-43738924 Msa1327200:CDS 25.0%
! TTGTTAACTCTTCAATTCAA+AGG + chr8_4:43738964-43738983 Msa1327200:CDS 25.0%
! TGTTTAATGAGTTTAATGCA+AGG + chr8_4:43739058-43739077 Msa1327200:CDS 25.0%
!!! AGAAGAAGAATGTTTTTGTA+GGG + chr8_4:43739088-43739107 Msa1327200:CDS 25.0%
!!! TTTTAAGAGCAAGTTGTTTT+TGG + chr8_4:43739113-43739132 Msa1327200:CDS 25.0%
!!! TTTAAGAGCAAGTTGTTTTT+GGG + chr8_4:43739114-43739133 Msa1327200:CDS 25.0%
!!! AAGTTGTTTTTGGGAATTAT+TGG + chr8_4:43739123-43739142 Msa1327200:CDS 25.0%
!!! AGAGAATGGATTATTTGTAT+TGG + chr8_4:43739219-43739238 Msa1327200:CDS 25.0%
!!! TGGATTATTTGTATTGGTTT+TGG + chr8_4:43739225-43739244 Msa1327200:CDS 25.0%
TCGTTCATGTTTGTGAAAAA+TGG - chr8_4:43736036-43736055 None:intergenic 30.0%
!!! TGTGTGTTATTGGTGTTTTT+TGG - chr8_4:43736081-43736100 None:intergenic 30.0%
!! TCTTTTGTTGTGTGTGTTAT+TGG - chr8_4:43736091-43736110 None:intergenic 30.0%
ATAACACACACAACAAAAGA+TGG + chr8_4:43736091-43736110 Msa1327200:CDS 30.0%
TATGAGAGTTTCTTGATCAA+TGG - chr8_4:43736238-43736257 None:intergenic 30.0%
ATTGACACACTTCAAAATCA+TGG + chr8_4:43736279-43736298 Msa1327200:CDS 30.0%
CTTAAAACCAACATTGAGTT+TGG + chr8_4:43736327-43736346 Msa1327200:CDS 30.0%
TTTGATACCAAACTCAATGT+TGG - chr8_4:43736337-43736356 None:intergenic 30.0%
!! ATTTTGGAGACATTGCAATT+AGG + chr8_4:43736373-43736392 Msa1327200:CDS 30.0%
!! TGAATGAAACTTTTAGAAGG+TGG - chr8_4:43736432-43736451 None:intergenic 30.0%
! GATGTTGTTTGTTTGAAGAT+TGG + chr8_4:43736735-43736754 Msa1327200:CDS 30.0%
!!! TTTCATCCTTTTTTGCTTTC+GGG + chr8_4:43736861-43736880 Msa1327200:CDS 30.0%
! TGCAAAAATGCTTGTTACAT+CGG + chr8_4:43736905-43736924 Msa1327200:CDS 30.0%
! TTATTCAACCTTGTTTGCAA+AGG - chr8_4:43736996-43737015 None:intergenic 30.0%
AATAAGCTAACATCATCGAT+CGG + chr8_4:43737011-43737030 Msa1327200:CDS 30.0%
ATCGAATCAATAGAACAACA+AGG - chr8_4:43737067-43737086 None:intergenic 30.0%
!! CTATTGATTCGATATTTCAC+TGG + chr8_4:43737074-43737093 Msa1327200:CDS 30.0%
GATGTGATGAATGCAATTAT+TGG + chr8_4:43737155-43737174 Msa1327200:CDS 30.0%
! TTCAATAGTACCTGTTTTGT+CGG - chr8_4:43737348-43737367 None:intergenic 30.0%
GTTCTTCAATTGATCAAAGA+AGG + chr8_4:43737582-43737601 Msa1327200:CDS 30.0%
!!! GATAGTATTGCTTTTTCTGT+TGG - chr8_4:43737684-43737703 None:intergenic 30.0%
CAGAAAAAGCAATACTATCT+TGG + chr8_4:43737685-43737704 Msa1327200:CDS 30.0%
AACTCAAAGAAGAAAAGAAG+TGG + chr8_4:43737780-43737799 Msa1327200:CDS 30.0%
ACAACAAAACTATTGCACAT+TGG + chr8_4:43737826-43737845 Msa1327200:CDS 30.0%
AAAACTATTGCACATTGGAA+AGG + chr8_4:43737831-43737850 Msa1327200:CDS 30.0%
ATGTGTTCAAGATACTATGA+TGG + chr8_4:43737876-43737895 Msa1327200:CDS 30.0%
TCAAGATACTATGATGGTTA+TGG + chr8_4:43737882-43737901 Msa1327200:CDS 30.0%
!! AATCATTTTGACATTCACAC+CGG - chr8_4:43738150-43738169 None:intergenic 30.0%
GTGAATGTCAAAATGATTAC+AGG + chr8_4:43738152-43738171 Msa1327200:CDS 30.0%
AGACACGTATGTATAAATAG+AGG - chr8_4:43738239-43738258 None:intergenic 30.0%
CTATAATCAGAGATAGAGTT+CGG - chr8_4:43738276-43738295 None:intergenic 30.0%
AAAGCTATAGCATTTGAATG+CGG + chr8_4:43738316-43738335 Msa1327200:CDS 30.0%
!! ACTTTTTCTAATCTCTCTTC+GGG - chr8_4:43738409-43738428 None:intergenic 30.0%
!! GACTTTTTCTAATCTCTCTT+CGG - chr8_4:43738410-43738429 None:intergenic 30.0%
AGTCGAAAAAATATCTGTGA+TGG + chr8_4:43738426-43738445 Msa1327200:CDS 30.0%
TCCATTTGACAAACTTCTAA+TGG + chr8_4:43738459-43738478 Msa1327200:CDS 30.0%
ACCATTAGAAGTTTGTCAAA+TGG - chr8_4:43738463-43738482 None:intergenic 30.0%
TTATCCATTAATTCCTTGGT+AGG - chr8_4:43738859-43738878 None:intergenic 30.0%
! TTCAAAGGTGAGGATATTTT+TGG + chr8_4:43738979-43738998 Msa1327200:CDS 30.0%
!!! TATTTTTGGTGTGACATCAA+AGG + chr8_4:43738993-43739012 Msa1327200:CDS 30.0%
TGAGTTTAATGCAAGGAAAT+TGG + chr8_4:43739065-43739084 Msa1327200:CDS 30.0%
!! GAGAAGAAGAATGTTTTTGT+AGG + chr8_4:43739087-43739106 Msa1327200:CDS 30.0%
AACTTCACAACAAAACCAAT+AGG - chr8_4:43739264-43739283 None:intergenic 30.0%
!! AGGTGTGATTTTCTGTTTCT+TGG - chr8_4:43736169-43736188 None:intergenic 35.0%
!! TGGTGGATTTAAATCAACCA+CGG - chr8_4:43736205-43736224 None:intergenic 35.0%
TGATCAATGGTAAAAGAGTG+TGG - chr8_4:43736225-43736244 None:intergenic 35.0%
GCAATTAGGAAACAAGTGTT+TGG + chr8_4:43736387-43736406 Msa1327200:CDS 35.0%
AGGTGGTTTCTTATAAGTGT+TGG - chr8_4:43736415-43736434 None:intergenic 35.0%
AAAGTGTAGCACAAACCAAA+AGG - chr8_4:43736494-43736513 None:intergenic 35.0%
AAGCAACATGGAATCAAAGA+AGG + chr8_4:43736531-43736550 Msa1327200:CDS 35.0%
AACATGGAATCAAAGAAGGT+TGG + chr8_4:43736535-43736554 Msa1327200:CDS 35.0%
!! ATCAAAGAAGGTTGGTATGA+TGG + chr8_4:43736543-43736562 Msa1327200:CDS 35.0%
AAATAACGATGAAAACAGCG+AGG - chr8_4:43736581-43736600 None:intergenic 35.0%
!! CAAATTGATTTGGTTCGAAG+TGG + chr8_4:43736672-43736691 Msa1327200:CDS 35.0%
!!! TTCATCCTTTTTTGCTTTCG+GGG + chr8_4:43736862-43736881 Msa1327200:CDS 35.0%
! AAAATGCTTGTTACATCGGT+TGG + chr8_4:43736909-43736928 Msa1327200:CDS 35.0%
ACTCACTTTGAATCAGATGA+AGG + chr8_4:43737494-43737513 Msa1327200:CDS 35.0%
TCACAAATCGAAATCAGGTT+CGG + chr8_4:43737632-43737651 Msa1327200:CDS 35.0%
!! GATTCTGAATTCGAGTTTTC+AGG + chr8_4:43737654-43737673 Msa1327200:CDS 35.0%
! AATACTATCTTGGGCTGTTT+TGG + chr8_4:43737695-43737714 Msa1327200:CDS 35.0%
!!! GGCTGTTTTGGAATTGAAAA+TGG + chr8_4:43737707-43737726 Msa1327200:CDS 35.0%
!! CGAAAGCATTATTCAAGGTA+TGG + chr8_4:43737944-43737963 Msa1327200:CDS 35.0%
AAGCATTATTCAAGGTATGG+CGG + chr8_4:43737947-43737966 Msa1327200:CDS 35.0%
TGGAGACAATAACAAGATGA+TGG + chr8_4:43738031-43738050 Msa1327200:CDS 35.0%
TTAACATTGTTAGGGCTTGT+CGG + chr8_4:43738068-43738087 Msa1327200:CDS 35.0%
CGAACTCTATCTCTGATTAT+AGG + chr8_4:43738274-43738293 Msa1327200:intron 35.0%
TATGGATACATTAGGTGCAT+TGG + chr8_4:43738816-43738835 Msa1327200:CDS 35.0%
AAAGCCTACCAAGGAATTAA+TGG + chr8_4:43738852-43738871 Msa1327200:CDS 35.0%
AACTCTTCAATTCAAAGGTG+AGG + chr8_4:43738969-43738988 Msa1327200:CDS 35.0%
!! GAGTTTTTGAAGAAGTTTGC+TGG + chr8_4:43739177-43739196 Msa1327200:CDS 35.0%
AAAGGTTGAATTGGAGAGAA+TGG + chr8_4:43739205-43739224 Msa1327200:CDS 35.0%
! AGAAATGGTTTATCTGAGAC+AGG - chr8_4:43739291-43739310 None:intergenic 35.0%
AAATCAACCACGGTGAAAGA+TGG - chr8_4:43736195-43736214 None:intergenic 40.0%
! TCAATGGTAAAAGAGTGTGG+TGG - chr8_4:43736222-43736241 None:intergenic 40.0%
GACACACTTCAAAATCATGG+TGG + chr8_4:43736282-43736301 Msa1327200:CDS 40.0%
! CAAAATCATGGTGGAGTTGA+AGG + chr8_4:43736291-43736310 Msa1327200:CDS 40.0%
GTTTGTGCTACACTTTCACT+TGG + chr8_4:43736498-43736517 Msa1327200:CDS 40.0%
!! AAAGAAGGTTGGTATGATGG+TGG + chr8_4:43736546-43736565 Msa1327200:CDS 40.0%
! GAAGATTGGTGATCAAGTAC+CGG + chr8_4:43736749-43736768 Msa1327200:CDS 40.0%
TCTACAAACAATCCATCAGC+CGG - chr8_4:43736771-43736790 None:intergenic 40.0%
!! GCTGATGGATTGTTTGTAGA+TGG + chr8_4:43736771-43736790 Msa1327200:CDS 40.0%
TTTGTCCCCGAAAGCAAAAA+AGG - chr8_4:43736870-43736889 None:intergenic 40.0%
AGGAAACACCTTTGCAAACA+AGG + chr8_4:43736985-43737004 Msa1327200:CDS 40.0%
ACATCATCGATCGGAAAAGT+TGG + chr8_4:43737020-43737039 Msa1327200:CDS 40.0%
! ATCGATCGGAAAAGTTGGTT+TGG + chr8_4:43737025-43737044 Msa1327200:CDS 40.0%
! TGTTGTTGTTGCAATTCCTG+AGG + chr8_4:43737202-43737221 Msa1327200:CDS 40.0%
! GTTGTTGTTGCAATTCCTGA+GGG + chr8_4:43737203-43737222 Msa1327200:CDS 40.0%
AGTGTCAATGTCACAGCTAA+TGG - chr8_4:43737233-43737252 None:intergenic 40.0%
AATGATGGCTGATCAAGCAA+TGG + chr8_4:43737271-43737290 Msa1327200:CDS 40.0%
! ACTATTTGCACCGACAAAAC+AGG + chr8_4:43737335-43737354 Msa1327200:CDS 40.0%
! AGATGAAGGTGACGAAGTTT+TGG + chr8_4:43737508-43737527 Msa1327200:CDS 40.0%
! GAATATGCACCCTTTTCCAA+TGG - chr8_4:43737546-43737565 None:intergenic 40.0%
GGTGTTCACAAATCGAAATC+AGG + chr8_4:43737627-43737646 Msa1327200:CDS 40.0%
!!! GAAGTGGAGTTTTGTTGAGA+AGG + chr8_4:43737796-43737815 Msa1327200:CDS 40.0%
GTTTCATCTGTGTCCTCATT+AGG - chr8_4:43738352-43738371 None:intergenic 40.0%
AAATTGTTCGCCTTCAACCA+CGG - chr8_4:43738374-43738393 None:intergenic 40.0%
AGATGGAACAAATGATGCAC+CGG + chr8_4:43738525-43738544 Msa1327200:CDS 40.0%
!! ATATCGGCTTCTTTCAATGC+CGG - chr8_4:43738547-43738566 None:intergenic 40.0%
GCATTGAAAGAAGCCGATAT+CGG + chr8_4:43738547-43738566 Msa1327200:CDS 40.0%
TCCCATTGAAAGACCGATAT+CGG - chr8_4:43738563-43738582 None:intergenic 40.0%
GGTCTTTCAATGGGAATTCA+AGG + chr8_4:43738568-43738587 Msa1327200:CDS 40.0%
TGTTATCAATATCGTAGCCG+CGG + chr8_4:43738738-43738757 Msa1327200:CDS 40.0%
TGCTGTTAATGGTACTTCAC+CGG - chr8_4:43738770-43738789 None:intergenic 40.0%
GGGAATTATTGGTGTGACAT+TGG + chr8_4:43739134-43739153 Msa1327200:CDS 40.0%
!! ATTGGTGCTTCAAGTTGTGA+TGG + chr8_4:43739152-43739171 Msa1327200:CDS 40.0%
!! AGAAGTTTGCTGGTACAGAA+AGG + chr8_4:43739187-43739206 Msa1327200:CDS 40.0%
CTGGTACAGAAAGGTTGAAT+TGG + chr8_4:43739196-43739215 Msa1327200:CDS 40.0%
!!! TTGGTTTTGGTGCTGTTTCT+TGG + chr8_4:43739238-43739257 Msa1327200:CDS 40.0%
GCTACACTTTCACTTGGCTT+TGG + chr8_4:43736504-43736523 Msa1327200:CDS 45.0%
! GGCTTTGGTATCAAGCAACA+TGG + chr8_4:43736519-43736538 Msa1327200:CDS 45.0%
! ATTTGGTTCGAAGTGGTCGT+CGG + chr8_4:43736679-43736698 Msa1327200:CDS 45.0%
! GTTGATGAATCGAGCATGAC+AGG + chr8_4:43736807-43736826 Msa1327200:CDS 45.0%
! GGTTGGTATGAACACGACAT+GGG + chr8_4:43736926-43736945 Msa1327200:CDS 45.0%
GTTGGTATGAACACGACATG+GGG + chr8_4:43736927-43736946 Msa1327200:CDS 45.0%
GATAAGCAATGACATCGACG+AGG + chr8_4:43736965-43736984 Msa1327200:CDS 45.0%
!! CGGAAAAGTTGGTTTGGTTG+TGG + chr8_4:43737031-43737050 Msa1327200:CDS 45.0%
GGGAACACAAAGAACGATAG+CGG + chr8_4:43737095-43737114 Msa1327200:CDS 45.0%
AGCGGAGTTAGAGAGTTCAA+CGG + chr8_4:43737113-43737132 Msa1327200:CDS 45.0%
ACTCTCTGCATGTGAGACTA+TGG + chr8_4:43737301-43737320 Msa1327200:CDS 45.0%
CTCTCTGCATGTGAGACTAT+GGG + chr8_4:43737302-43737321 Msa1327200:CDS 45.0%
!!! AAGGTGACGAAGTTTTGGCT+TGG + chr8_4:43737513-43737532 Msa1327200:CDS 45.0%
! TGGTCTAGAGCCATTGGAAA+AGG + chr8_4:43737533-43737552 Msa1327200:CDS 45.0%
! GGTCTAGAGCCATTGGAAAA+GGG + chr8_4:43737534-43737553 Msa1327200:CDS 45.0%
GGAGTTGCACTCAATACAAC+TGG + chr8_4:43737603-43737622 Msa1327200:CDS 45.0%
GTTGCACTCAATACAACTGG+AGG + chr8_4:43737606-43737625 Msa1327200:CDS 45.0%
GTTAGGGCTTGTCGGTATTA+AGG + chr8_4:43738076-43738095 Msa1327200:CDS 45.0%
ATTAAGGATCCATGTCGTCC+AGG + chr8_4:43738092-43738111 Msa1327200:CDS 45.0%
TTAAGGATCCATGTCGTCCA+GGG + chr8_4:43738093-43738112 Msa1327200:CDS 45.0%
CGGAATTCTCCAACCTAATG+AGG + chr8_4:43738336-43738355 Msa1327200:CDS 45.0%
CATCTGTGTCCTCATTAGGT+TGG - chr8_4:43738348-43738367 None:intergenic 45.0%
ACAGATGAAACCGTGGTTGA+AGG + chr8_4:43738361-43738380 Msa1327200:CDS 45.0%
CTCCTGTTACAGCAACTACA+TGG - chr8_4:43738507-43738526 None:intergenic 45.0%
GTAGTTGCTGTAACAGGAGA+TGG + chr8_4:43738508-43738527 Msa1327200:CDS 45.0%
AGCCGATATCGGTCTTTCAA+TGG + chr8_4:43738558-43738577 Msa1327200:CDS 45.0%
GCCGATATCGGTCTTTCAAT+GGG + chr8_4:43738559-43738578 Msa1327200:CDS 45.0%
TGCAACTGAAAAGCCTACCA+AGG + chr8_4:43738843-43738862 Msa1327200:CDS 45.0%
!! AGAGGCTTTGTTCTACCAAC+TGG - chr8_4:43738886-43738905 None:intergenic 45.0%
ACCTACTCCATCTTTCACCG+TGG + chr8_4:43736185-43736204 Msa1327200:CDS 50.0%
ACCACGGTGAAAGATGGAGT+AGG - chr8_4:43736189-43736208 None:intergenic 50.0%
! TCGAAGTGGTCGTCGGTTAA+AGG + chr8_4:43736686-43736705 Msa1327200:CDS 50.0%
TCGGGGACAAAAGTTGTCGA+TGG + chr8_4:43736879-43736898 Msa1327200:CDS 50.0%
! CGGTTGGTATGAACACGACA+TGG + chr8_4:43736925-43736944 Msa1327200:CDS 50.0%
ACAGCTAATGGCAAACCCTC+AGG - chr8_4:43737221-43737240 None:intergenic 50.0%
!! TTGGCTTGGTCTAGAGCCAT+TGG + chr8_4:43737527-43737546 Msa1327200:CDS 50.0%
TTGGAAAGGAGCAGCAGAGA+TGG + chr8_4:43737845-43737864 Msa1327200:CDS 50.0%
AAAGTCGCAGACGAAGAGCT+TGG + chr8_4:43738005-43738024 Msa1327200:CDS 50.0%
TAAGGATCCATGTCGTCCAG+GGG + chr8_4:43738094-43738113 Msa1327200:CDS 50.0%
TGACATTCACACCGGCATGT+TGG - chr8_4:43738142-43738161 None:intergenic 50.0%
TGAGGACACAGATGAAACCG+TGG + chr8_4:43738354-43738373 Msa1327200:CDS 50.0%
GGCCATGTAGTTGCTGTAAC+AGG + chr8_4:43738502-43738521 Msa1327200:CDS 50.0%
!! GGTGCTGTTTCTTGGCCTAT+TGG + chr8_4:43739246-43739265 Msa1327200:CDS 50.0%
!! GGTGATCAAGTACCGGCTGA+TGG + chr8_4:43736756-43736775 Msa1327200:CDS 55.0%
GCAGACGAAGAGCTTGGAGA+TGG + chr8_4:43738011-43738030 Msa1327200:CDS 55.0%
TTCTTCACCCCTGGACGACA+TGG - chr8_4:43738104-43738123 None:intergenic 55.0%
TCGTCCAGGGGTGAAGAAAG+CGG + chr8_4:43738106-43738125 Msa1327200:CDS 55.0%
GTGGAAGCTTGCCAACATGC+CGG + chr8_4:43738128-43738147 Msa1327200:CDS 55.0%
! TACTTCACCGGCTGAAACCG+CGG - chr8_4:43738758-43738777 None:intergenic 55.0%
TCCAGGGGTGAAGAAAGCGG+TGG + chr8_4:43738109-43738128 Msa1327200:CDS 60.0%
TCCACCGCTTTCTTCACCCC+TGG - chr8_4:43738113-43738132 None:intergenic 60.0%
ATCGTAGCCGCGGTTTCAGC+CGG + chr8_4:43738748-43738767 Msa1327200:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 43736024 43739338 43736024 ID=Msa1327200;Name=Msa1327200
chr8_4 mRNA 43736024 43739338 43736024 ID=Msa1327200-mRNA-1;Parent=Msa1327200;Name=Msa1327200-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|1|0|1|1|1|3|0|1019
chr8_4 exon 43736024 43737356 43736024 ID=Msa1327200-mRNA-1:exon:12397;Parent=Msa1327200-mRNA-1
chr8_4 exon 43737490 43738173 43737490 ID=Msa1327200-mRNA-1:exon:12398;Parent=Msa1327200-mRNA-1
chr8_4 exon 43738296 43739338 43738296 ID=Msa1327200-mRNA-1:exon:12399;Parent=Msa1327200-mRNA-1
chr8_4 CDS 43736024 43737356 43736024 ID=Msa1327200-mRNA-1:cds;Parent=Msa1327200-mRNA-1
chr8_4 CDS 43737490 43738173 43737490 ID=Msa1327200-mRNA-1:cds;Parent=Msa1327200-mRNA-1
chr8_4 CDS 43738296 43739338 43738296 ID=Msa1327200-mRNA-1:cds;Parent=Msa1327200-mRNA-1
Gene Sequence

>Msa1327200

ATGTCAATGCCATTTTTCACAAACATGAACGACATTGAGACACTTCTCAATGCACCAAAAAACACCAATAACACACACAACAAAAGATGGCACAATGCATTCATGAAAATCTATTGTTCTAGGGCTTTCATGTTACATTTTACCAAGAAACAGAAAATCACACCTACTCCATCTTTCACCGTGGTTGATTTAAATCCACCACACTCTTTTACCATTGATCAAGAAACTCTCATAGATATTGTCAAAGAGAAAAACATTGACACACTTCAAAATCATGGTGGAGTTGAAGGTGTTGCTTCATCACTTAAAACCAACATTGAGTTTGGTATCAAAAGTGATGATACTAATGATTTTGGAGACATTGCAATTAGGAAACAAGTGTTTGGTTCCAACACTTATAAGAAACCACCTTCTAAAAGTTTCATTCATTTTGTTGTTGAAGCTTTTAAGGATGTAACAATTTTAATCCTTTTGGTTTGTGCTACACTTTCACTTGGCTTTGGTATCAAGCAACATGGAATCAAAGAAGGTTGGTATGATGGTGGCAGCATTTTCCTCGCTGTTTTCATCGTTATTTCGATGTCATCAATAAGCAACTTCAAACAAAACAAACAATTTGATAAATTGTCTCAATTTAGCAATGACATTCAAATTGATTTGGTTCGAAGTGGTCGTCGGTTAAAGGTATCAATATTTGATATTGTTGTTGGTGATGTTGTTTGTTTGAAGATTGGTGATCAAGTACCGGCTGATGGATTGTTTGTAGATGGACATTCGTTGAGAGTTGATGAATCGAGCATGACAGGTGAGAGCGATCATGTAGAGATAAATCGAAATTTTCATCCTTTTTTGCTTTCGGGGACAAAAGTTGTCGATGGATATGCAAAAATGCTTGTTACATCGGTTGGTATGAACACGACATGGGGACAAATGATGAGTTCGATAAGCAATGACATCGACGAGGAAACACCTTTGCAAACAAGGTTGAATAAGCTAACATCATCGATCGGAAAAGTTGGTTTGGTTGTGGCATTTTTAGTCCTTGTTGTTCTATTGATTCGATATTTCACTGGGAACACAAAGAACGATAGCGGAGTTAGAGAGTTCAACGGTAGAAAGACTAGTTTTGATGATGTGATGAATGCAATTATTGGAATTATTGCTGATGCTGTTACTATTGTTGTTGTTGCAATTCCTGAGGGTTTGCCATTAGCTGTGACATTGACACTTGCTTATTCAATGAAGAAAATGATGGCTGATCAAGCAATGGTGAGAAAACTCTCTGCATGTGAGACTATGGGATCTGCTACTACTATTTGCACCGACAAAACAGGTACACTCACTTTGAATCAGATGAAGGTGACGAAGTTTTGGCTTGGTCTAGAGCCATTGGAAAAGGGTGCATATTCAAATGTTGATCCATATGTTCTTCAATTGATCAAAGAAGGAGTTGCACTCAATACAACTGGAGGTGTTCACAAATCGAAATCAGGTTCGGATTCTGAATTCGAGTTTTCAGGAAGTCCAACAGAAAAAGCAATACTATCTTGGGCTGTTTTGGAATTGAAAATGGATATGGAGCACTTGACAAAAAGTTGTTCTATTATTCAAGTTGAGACTTTCAACTCAAAGAAGAAAAGAAGTGGAGTTTTGTTGAGAAGGAATGTTGACAACAAAACTATTGCACATTGGAAAGGAGCAGCAGAGATGGTTCTAAGAATGTGTTCAAGATACTATGATGGTTATGGAATTTTGAAAGATCTTGACAATGAAAGCATGTCAAAATTCGAAAGCATTATTCAAGGTATGGCGGCTAGTAGTCTTCGTTGCATCGCTTTAGCATATACAAAAGTCGCAGACGAAGAGCTTGGAGATGGAGACAATAACAAGATGATGGTAAAAGACAATGATTTAACATTGTTAGGGCTTGTCGGTATTAAGGATCCATGTCGTCCAGGGGTGAAGAAAGCGGTGGAAGCTTGCCAACATGCCGGTGTGAATGTCAAAATGATTACAGGTGACAATGTTTTCACTGCGAAAGCTATAGCATTTGAATGCGGAATTCTCCAACCTAATGAGGACACAGATGAAACCGTGGTTGAAGGCGAACAATTTCGCAACTTCACACCCGAAGAGAGATTAGAAAAAGTCGAAAAAATATCTGTGATGGCAAGATCTTCTCCATTTGACAAACTTCTAATGGTTCAATGTTTAAAACAAAAAGGCCATGTAGTTGCTGTAACAGGAGATGGAACAAATGATGCACCGGCATTGAAAGAAGCCGATATCGGTCTTTCAATGGGAATTCAAGGTACAGAAGTAGCAAAAGAGAGTTCAGATATTGTTATATTAGATGACAATTTTGCATCTATAGTAACAGTTTTAAATTGGGGAAGATGTGTTTACAACAACATTCAAAAGTTCATTCAATTTCAATTAACAGTGAATGTGGCAGCACTTGTTATCAATATCGTAGCCGCGGTTTCAGCCGGTGAAGTACCATTAACAGCAGTACAATTATTATGGGTCAATTTGATTATGGATACATTAGGTGCATTGGCTCTTGCAACTGAAAAGCCTACCAAGGAATTAATGGATAAAAAACCAGTTGGTAGAACAAAGCCTCTTATAACTAACATTATGTGGAGGAATCTTGTGTCACAAGCTTTGTACCAAATAGTAATTTTGTTAACTCTTCAATTCAAAGGTGAGGATATTTTTGGTGTGACATCAAAGGTAAATGACACATTGATATTTAATACATTTGTTCTATGTCAAGTGTTTAATGAGTTTAATGCAAGGAAATTGGAGAAGAAGAATGTTTTTGTAGGGATTTTTAAGAGCAAGTTGTTTTTGGGAATTATTGGTGTGACATTGGTGCTTCAAGTTGTGATGGTTGAGTTTTTGAAGAAGTTTGCTGGTACAGAAAGGTTGAATTGGAGAGAATGGATTATTTGTATTGGTTTTGGTGCTGTTTCTTGGCCTATTGGTTTTGTTGTGAAGTTGATACCTGTCTCAGATAAACCATTTCTTGATTTTTTGAGTATGAAGAAGACTTAG
Protein sequence

>Msa1327200

MSMPFFTNMNDIETLLNAPKNTNNTHNKRWHNAFMKIYCSRAFMLHFTKKQKITPTPSFTVVDLNPPHSFTIDQETLIDIVKEKNIDTLQNHGGVEGVASSLKTNIEFGIKSDDTNDFGDIAIRKQVFGSNTYKKPPSKSFIHFVVEAFKDVTILILLVCATLSLGFGIKQHGIKEGWYDGGSIFLAVFIVISMSSISNFKQNKQFDKLSQFSNDIQIDLVRSGRRLKVSIFDIVVGDVVCLKIGDQVPADGLFVDGHSLRVDESSMTGESDHVEINRNFHPFLLSGTKVVDGYAKMLVTSVGMNTTWGQMMSSISNDIDEETPLQTRLNKLTSSIGKVGLVVAFLVLVVLLIRYFTGNTKNDSGVREFNGRKTSFDDVMNAIIGIIADAVTIVVVAIPEGLPLAVTLTLAYSMKKMMADQAMVRKLSACETMGSATTICTDKTGTLTLNQMKVTKFWLGLEPLEKGAYSNVDPYVLQLIKEGVALNTTGGVHKSKSGSDSEFEFSGSPTEKAILSWAVLELKMDMEHLTKSCSIIQVETFNSKKKRSGVLLRRNVDNKTIAHWKGAAEMVLRMCSRYYDGYGILKDLDNESMSKFESIIQGMAASSLRCIALAYTKVADEELGDGDNNKMMVKDNDLTLLGLVGIKDPCRPGVKKAVEACQHAGVNVKMITGDNVFTAKAIAFECGILQPNEDTDETVVEGEQFRNFTPEERLEKVEKISVMARSSPFDKLLMVQCLKQKGHVVAVTGDGTNDAPALKEADIGLSMGIQGTEVAKESSDIVILDDNFASIVTVLNWGRCVYNNIQKFIQFQLTVNVAALVINIVAAVSAGEVPLTAVQLLWVNLIMDTLGALALATEKPTKELMDKKPVGRTKPLITNIMWRNLVSQALYQIVILLTLQFKGEDIFGVTSKVNDTLIFNTFVLCQVFNEFNARKLEKKNVFVGIFKSKLFLGIIGVTLVLQVVMVEFLKKFAGTERLNWREWIICIGFGAVSWPIGFVVKLIPVSDKPFLDFLSMKKT*