AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1329620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1329620 MtrunA17_Chr4g0066451 98.165 218 4 0 1 218 1 218 1.06e-160 442
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1329620 AT4G15520.1 76.147 218 49 2 3 217 4 221 5.41e-122 345

Find 57 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTATTGTGCTTCATCAATT+TGG 0.178884 8_4:+48333621 Msa1329620:CDS
CTTGATGCATTGTTTGTTGA+TGG 0.220225 8_4:+48335583 Msa1329620:CDS
CAATTGACAGATTGTAGTTT+AGG 0.238093 8_4:+48335636 Msa1329620:three_prime_UTR
TTAAAAGGATGTTGATTAAC+AGG 0.260098 8_4:-48333465 None:intergenic
AAAGCAGTACTTCTCTTAAA+AGG 0.284242 8_4:-48333480 None:intergenic
TTTGTTTATATTCCGCAATA+TGG 0.296529 8_4:+48333561 Msa1329620:CDS
TGCCATTTGCTCTACTGATA+TGG 0.299194 8_4:+48335898 Msa1329620:three_prime_UTR
CTTAGGTATCTAATGTTGTT+TGG 0.325239 8_4:+48335697 Msa1329620:three_prime_UTR
CATTGTTTGTTGATGGTTGA+TGG 0.330834 8_4:+48335590 Msa1329620:CDS
AATTGACAGATTGTAGTTTA+GGG 0.333797 8_4:+48335637 Msa1329620:three_prime_UTR
CGAAGAGATTTCAACAGTTT+CGG 0.342769 8_4:+48333321 Msa1329620:CDS
AGAAGTACTGCTTTCTTGCT+TGG 0.377151 8_4:+48333489 Msa1329620:CDS
TAGAAGAGAACCGACATCTT+AGG 0.393257 8_4:+48335680 Msa1329620:three_prime_UTR
ATTGGCGGCATTCTCTCTTC+TGG 0.397261 8_4:-48335515 None:intergenic
AGAAGATTTCTACTGTCATT+AGG 0.401312 8_4:+48335762 Msa1329620:three_prime_UTR
CAGAGTTGATACTAACAAAA+TGG 0.406161 8_4:+48333191 Msa1329620:exon
GCACGAAGCGCAACAGCATT+CGG 0.421833 8_4:+48333273 Msa1329620:CDS
GCTTTCTTGCTTGGTAATGA+AGG 0.426363 8_4:+48333498 Msa1329620:CDS
TTGCTTGGTAATGAAGGTTC+CGG 0.428513 8_4:+48333504 Msa1329620:CDS
AACCATATCAGTAGAGCAAA+TGG 0.443015 8_4:-48335900 None:intergenic
TTGTTTATATTCCGCAATAT+GGG 0.446663 8_4:+48333562 Msa1329620:CDS
TCGACCAACGAGGATTAGTT+CGG 0.466321 8_4:-48333301 None:intergenic
ATATATACAGAGGCATTAGC+TGG 0.474340 8_4:+48335735 Msa1329620:three_prime_UTR
GACAAAGATTGTGACATTTG+CGG 0.478875 8_4:+48333417 Msa1329620:CDS
ACACTCCTTCGTCGAAAGAC+CGG 0.479365 8_4:-48333523 None:intergenic
ATTGTGCTTCATCAATTTGG+AGG 0.481305 8_4:+48333624 Msa1329620:CDS
GCAAATGGCAGTATAATAGT+TGG 0.481426 8_4:-48335885 None:intergenic
TATTTGTTGCAGAGAGAAAA+CGG 0.487646 8_4:-48333136 None:intergenic
AACGGCTACCGCGTTGCGAA+AGG 0.502306 8_4:-48333118 None:intergenic
AGCTTCTTCAAAGAAACCAT+TGG 0.515730 8_4:-48335533 None:intergenic
TTTCGTCGACCATGTTTCAC+AGG 0.524298 8_4:-48335448 None:intergenic
ACGGCTACCGCGTTGCGAAA+GGG 0.531792 8_4:-48333117 None:intergenic
TATATTCCGCAATATGGGTG+TGG 0.552488 8_4:+48333567 Msa1329620:CDS
TTCTTCAAAGAAACCATTGG+CGG 0.556928 8_4:-48335530 None:intergenic
CAACAACTGTCACTAGTGAC+AGG 0.557401 8_4:+48335812 Msa1329620:three_prime_UTR
GACAAAGCAAATTGCACGGA+AGG 0.567099 8_4:-48333167 None:intergenic
TTCAACAGTTTCGGTAACCA+TGG 0.567269 8_4:+48333330 Msa1329620:CDS
GGGCTTAAATGCTTCAGTTG+CGG 0.602530 8_4:+48335657 Msa1329620:three_prime_UTR
AGAAGAGAGAATGCCGCCAA+TGG 0.603778 8_4:+48335517 Msa1329620:CDS
ACATTAGATACCTAAGATGT+CGG 0.604714 8_4:-48335690 None:intergenic
TTGTACACAACATAGCAAAA+CGG 0.606644 8_4:+48333232 Msa1329620:CDS
AGGTTCCGGTCTTTCGACGA+AGG 0.609762 8_4:+48333518 Msa1329620:CDS
CATCAACAAACAATGCATCA+AGG 0.612565 8_4:-48335582 None:intergenic
GCAGAGAGACCTGTGAAACA+TGG 0.616817 8_4:+48335439 Msa1329620:CDS
TATATACAGAGGCATTAGCT+GGG 0.617803 8_4:+48335736 Msa1329620:three_prime_UTR
GTATCCGAACTAATCCTCGT+TGG 0.630687 8_4:+48333297 Msa1329620:CDS
GAAGGTGGTTTGAAGAACCA+TGG 0.643878 8_4:-48333347 None:intergenic
CTGCGACAAAGCAAATTGCA+CGG 0.652456 8_4:-48333171 None:intergenic
GAAAGTGACGAAATCGAAGG+TGG 0.672321 8_4:-48333362 None:intergenic
TGAAATCTCTTCGACCAACG+AGG 0.674642 8_4:-48333311 None:intergenic
AGTGAAAGTGACGAAATCGA+AGG 0.683890 8_4:-48333365 None:intergenic
TGGTCGACGAAATTACTGCA+CGG 0.684868 8_4:+48335459 Msa1329620:CDS
ACTGACTATTATATATACAG+AGG 0.686318 8_4:+48335725 Msa1329620:three_prime_UTR
TTTGCTGAGAGATCTCGTGA+TGG 0.690113 8_4:+48335400 Msa1329620:CDS
GCAGTACCACACCCATATTG+CGG 0.695525 8_4:-48333573 None:intergenic
ATAGCAAAACGGCACAACGT+AGG 0.696815 8_4:+48333243 Msa1329620:CDS
CAACAAACAATGCATCAAGG+AGG 0.748014 8_4:-48335579 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTGTATGTAAAAAAA+AGG - chr8_4:48334429-48334448 None:intergenic 10.0%
!!! AATAAATTTGCATAATTTTT+TGG - chr8_4:48334605-48334624 None:intergenic 10.0%
!!! AAATTTGCACTTAGATTTTT+GGG + chr8_4:48334011-48334030 Msa1329620:intron 20.0%
!!! ATTCTTTGAAATCTTGATTT+TGG + chr8_4:48334041-48334060 Msa1329620:intron 20.0%
!!! TTCTTTGAAATCTTGATTTT+GGG + chr8_4:48334042-48334061 Msa1329620:intron 20.0%
!! GGAAATGTTATTGATATATT+GGG - chr8_4:48334233-48334252 None:intergenic 20.0%
!! AGGAAATGTTATTGATATAT+TGG - chr8_4:48334234-48334253 None:intergenic 20.0%
!!! TTTTTGGTTACATTTAGATT+TGG + chr8_4:48335187-48335206 Msa1329620:intron 20.0%
!! TTAAAAGGATGTTGATTAAC+AGG - chr8_4:48333468-48333487 None:intergenic 25.0%
! TTTGTTTATATTCCGCAATA+TGG + chr8_4:48333561-48333580 Msa1329620:CDS 25.0%
! TTGTTTATATTCCGCAATAT+GGG + chr8_4:48333562-48333581 Msa1329620:CDS 25.0%
!!! GAAATTGATTTTGGTACATT+TGG - chr8_4:48333950-48333969 None:intergenic 25.0%
!!! TGATGACTTGAAATTGATTT+TGG - chr8_4:48333959-48333978 None:intergenic 25.0%
!!! GCAAATTTGAATTCACTTTT+GGG - chr8_4:48333999-48334018 None:intergenic 25.0%
!! TGCAAATTTGAATTCACTTT+TGG - chr8_4:48334000-48334019 None:intergenic 25.0%
!! CAAATTTGCACTTAGATTTT+TGG + chr8_4:48334010-48334029 Msa1329620:intron 25.0%
!! CTGAAAAGGTATATAAACAT+GGG - chr8_4:48334124-48334143 None:intergenic 25.0%
!! ACTGAAAAGGTATATAAACA+TGG - chr8_4:48334125-48334144 None:intergenic 25.0%
!! CTTAATATTGAAGGACATTT+TGG + chr8_4:48334275-48334294 Msa1329620:intron 25.0%
!! TATGTAAAAAAAAGGCATGT+TGG - chr8_4:48334421-48334440 None:intergenic 25.0%
!!! TTTTTTGGAAGTGCTATAAT+AGG - chr8_4:48334590-48334609 None:intergenic 25.0%
! ACTACAATAAGACATTAACA+AGG + chr8_4:48334633-48334652 Msa1329620:intron 25.0%
!! AAGTTTTTCACTAAAAGAAC+TGG - chr8_4:48334689-48334708 None:intergenic 25.0%
!! GTTCTTTTAGTGAAAAACTT+TGG + chr8_4:48334689-48334708 Msa1329620:intron 25.0%
!!! ATCATGAAAGTTTTAGTCAT+GGG + chr8_4:48334742-48334761 Msa1329620:intron 25.0%
! GAAAAACATGTTATATAGTC+TGG + chr8_4:48334901-48334920 Msa1329620:intron 25.0%
!!! TTGTTTATGTGCTCATTTTT+TGG + chr8_4:48335171-48335190 Msa1329620:intron 25.0%
! AACAAAACCAATATACAGTT+GGG - chr8_4:48335224-48335243 None:intergenic 25.0%
! TTAAGTAAAAAGATGCAGTT+GGG - chr8_4:48335280-48335299 None:intergenic 25.0%
! ATTAAGTAAAAAGATGCAGT+TGG - chr8_4:48335281-48335300 None:intergenic 25.0%
! AACCAATATCATAATGAATG+AGG - chr8_4:48335307-48335326 None:intergenic 25.0%
! AATTGACAGATTGTAGTTTA+GGG + chr8_4:48335637-48335656 Msa1329620:three_prime_UTR 25.0%
! ACTGACTATTATATATACAG+AGG + chr8_4:48335725-48335744 Msa1329620:three_prime_UTR 25.0%
! TATTTGTTGCAGAGAGAAAA+CGG - chr8_4:48333139-48333158 None:intergenic 30.0%
!! CAGAGTTGATACTAACAAAA+TGG + chr8_4:48333191-48333210 Msa1329620:exon 30.0%
TTGTACACAACATAGCAAAA+CGG + chr8_4:48333232-48333251 Msa1329620:CDS 30.0%
AAAGCAGTACTTCTCTTAAA+AGG - chr8_4:48333483-48333502 None:intergenic 30.0%
TCTATTGTGCTTCATCAATT+TGG + chr8_4:48333621-48333640 Msa1329620:CDS 30.0%
! GTTGTGGTGATATTTTATGT+AGG + chr8_4:48333718-48333737 Msa1329620:intron 30.0%
!! TAACCTGCAAAAGTTGATTT+TGG + chr8_4:48333748-48333767 Msa1329620:intron 30.0%
AAGAGAAAACGATTTATGTC+TGG + chr8_4:48333798-48333817 Msa1329620:intron 30.0%
!! AATCTTGATTTTGGGAGAAT+TGG + chr8_4:48334050-48334069 Msa1329620:intron 30.0%
TAACATTCAACAACCAACTT+GGG + chr8_4:48334087-48334106 Msa1329620:intron 30.0%
!! ATCAGTTGGTGAAGAAAATA+TGG + chr8_4:48334175-48334194 Msa1329620:intron 30.0%
!! GTGTTGCTTCTTAATATTGA+AGG + chr8_4:48334266-48334285 Msa1329620:intron 30.0%
ATGAGGTGAAAAATTCATTG+AGG + chr8_4:48334311-48334330 Msa1329620:intron 30.0%
TGAGGTGAAAAATTCATTGA+GGG + chr8_4:48334312-48334331 Msa1329620:intron 30.0%
! TATGTAGCATATAGTGTTGA+AGG - chr8_4:48334484-48334503 None:intergenic 30.0%
! CTTTTAGTGAAAAACTTTGG+TGG + chr8_4:48334692-48334711 Msa1329620:intron 30.0%
!! CATCATGAAAGTTTTAGTCA+TGG + chr8_4:48334741-48334760 Msa1329620:intron 30.0%
! AACGTAAATTAGTCTGTAAC+TGG + chr8_4:48335078-48335097 Msa1329620:intron 30.0%
GTCATATGTTATCACTTACA+AGG - chr8_4:48335110-48335129 None:intergenic 30.0%
ATAACATATGACTACTGTAG+TGG + chr8_4:48335118-48335137 Msa1329620:intron 30.0%
ATGAGCACATAAACAACAAA+GGG - chr8_4:48335167-48335186 None:intergenic 30.0%
AATGAGCACATAAACAACAA+AGG - chr8_4:48335168-48335187 None:intergenic 30.0%
TCATATTCCCAACTGTATAT+TGG + chr8_4:48335214-48335233 Msa1329620:intron 30.0%
CAACAAAACCAATATACAGT+TGG - chr8_4:48335225-48335244 None:intergenic 30.0%
CACCTCATTCATTATGATAT+TGG + chr8_4:48335302-48335321 Msa1329620:intron 30.0%
!!! TTCACTTTTTTTGTCAGTCT+GGG + chr8_4:48335372-48335391 Msa1329620:intron 30.0%
CAATTGACAGATTGTAGTTT+AGG + chr8_4:48335636-48335655 Msa1329620:three_prime_UTR 30.0%
ACATTAGATACCTAAGATGT+CGG - chr8_4:48335693-48335712 None:intergenic 30.0%
CTTAGGTATCTAATGTTGTT+TGG + chr8_4:48335697-48335716 Msa1329620:three_prime_UTR 30.0%
AGAAGATTTCTACTGTCATT+AGG + chr8_4:48335762-48335781 Msa1329620:three_prime_UTR 30.0%
CGAAGAGATTTCAACAGTTT+CGG + chr8_4:48333321-48333340 Msa1329620:CDS 35.0%
GACAAAGATTGTGACATTTG+CGG + chr8_4:48333417-48333436 Msa1329620:CDS 35.0%
ATTGTGCTTCATCAATTTGG+AGG + chr8_4:48333624-48333643 Msa1329620:CDS 35.0%
! CAACCAAAATCAACTTTTGC+AGG - chr8_4:48333754-48333773 None:intergenic 35.0%
!! TTTGAGTGCTGAATCACATT+TGG + chr8_4:48333854-48333873 Msa1329620:intron 35.0%
! CAAGTCATCAGAATCACTTT+TGG + chr8_4:48333969-48333988 Msa1329620:intron 35.0%
GTAACATTCAACAACCAACT+TGG + chr8_4:48334086-48334105 Msa1329620:intron 35.0%
AGGATTCGTGAAAACTGAAA+AGG - chr8_4:48334138-48334157 None:intergenic 35.0%
TGAAGAAAATATGGAGTTGC+TGG + chr8_4:48334184-48334203 Msa1329620:intron 35.0%
AGCAACACTCACTCATAAAA+AGG - chr8_4:48334254-48334273 None:intergenic 35.0%
!! TATTGAAGGACATTTTGGTG+TGG + chr8_4:48334280-48334299 Msa1329620:intron 35.0%
!! ATTGAAGGACATTTTGGTGT+GGG + chr8_4:48334281-48334300 Msa1329620:intron 35.0%
GACTTATTTCAACTGCTATG+CGG + chr8_4:48334450-48334469 Msa1329620:intron 35.0%
TTAGTGAAAAACTTTGGTGG+TGG + chr8_4:48334695-48334714 Msa1329620:intron 35.0%
GTGTCAAAGAAACAGACTAA+GGG - chr8_4:48334990-48335009 None:intergenic 35.0%
AGTGTCAAAGAAACAGACTA+AGG - chr8_4:48334991-48335010 None:intergenic 35.0%
!!! GTTCACTTTTTTTGTCAGTC+TGG + chr8_4:48335371-48335390 Msa1329620:intron 35.0%
TTCTTCAAAGAAACCATTGG+CGG - chr8_4:48335533-48335552 None:intergenic 35.0%
AGCTTCTTCAAAGAAACCAT+TGG - chr8_4:48335536-48335555 None:intergenic 35.0%
CATCAACAAACAATGCATCA+AGG - chr8_4:48335585-48335604 None:intergenic 35.0%
! CTTGATGCATTGTTTGTTGA+TGG + chr8_4:48335583-48335602 Msa1329620:CDS 35.0%
!! CATTGTTTGTTGATGGTTGA+TGG + chr8_4:48335590-48335609 Msa1329620:CDS 35.0%
!! ATATATACAGAGGCATTAGC+TGG + chr8_4:48335735-48335754 Msa1329620:three_prime_UTR 35.0%
!! TATATACAGAGGCATTAGCT+GGG + chr8_4:48335736-48335755 Msa1329620:three_prime_UTR 35.0%
GCAAATGGCAGTATAATAGT+TGG - chr8_4:48335888-48335907 None:intergenic 35.0%
AACCATATCAGTAGAGCAAA+TGG - chr8_4:48335903-48335922 None:intergenic 35.0%
TTCAACAGTTTCGGTAACCA+TGG + chr8_4:48333330-48333349 Msa1329620:CDS 40.0%
AGTGAAAGTGACGAAATCGA+AGG - chr8_4:48333368-48333387 None:intergenic 40.0%
!! AGAAGTACTGCTTTCTTGCT+TGG + chr8_4:48333489-48333508 Msa1329620:CDS 40.0%
!! GCTTTCTTGCTTGGTAATGA+AGG + chr8_4:48333498-48333517 Msa1329620:CDS 40.0%
!! TTGCTTGGTAATGAAGGTTC+CGG + chr8_4:48333504-48333523 Msa1329620:CDS 40.0%
TATATTCCGCAATATGGGTG+TGG + chr8_4:48333567-48333586 Msa1329620:CDS 40.0%
TTCGCGATAAAAGAACGTTG+TGG + chr8_4:48333702-48333721 Msa1329620:intron 40.0%
GATTTATGTCTGGATACTCC+CGG + chr8_4:48333808-48333827 Msa1329620:intron 40.0%
ATTTATGTCTGGATACTCCC+GGG + chr8_4:48333809-48333828 Msa1329620:intron 40.0%
GGATAAAAATCCTCCCAAGT+TGG - chr8_4:48334103-48334122 None:intergenic 40.0%
GATCTCGCAAGGATTACTTA+AGG - chr8_4:48334158-48334177 None:intergenic 40.0%
TTGGTGTGGGATTGAATATG+AGG + chr8_4:48334294-48334313 Msa1329620:intron 40.0%
AGCGGAAAAGAGAGAATTTC+AGG + chr8_4:48334359-48334378 Msa1329620:intron 40.0%
GCGGAAAAGAGAGAATTTCA+GGG + chr8_4:48334360-48334379 Msa1329620:intron 40.0%
! AGCATATAGTGTTGAAGGTG+CGG - chr8_4:48334479-48334498 None:intergenic 40.0%
TCCGCCATTAATAACACTGA+TGG + chr8_4:48334535-48334554 Msa1329620:intron 40.0%
! CCCATCAGTGTTATTAATGG+CGG - chr8_4:48334539-48334558 None:intergenic 40.0%
CCGCCATTAATAACACTGAT+GGG + chr8_4:48334536-48334555 Msa1329620:intron 40.0%
! GTGCCCATCAGTGTTATTAA+TGG - chr8_4:48334542-48334561 None:intergenic 40.0%
CACTAAAAGAACTGGCTACA+AGG - chr8_4:48334681-48334700 None:intergenic 40.0%
!! GTTTTAGTCATGGGTGTGTT+TGG + chr8_4:48334751-48334770 Msa1329620:intron 40.0%
TTTGGATTGAGGGTTTAGGA+GGG - chr8_4:48334963-48334982 None:intergenic 40.0%
TGTGTTTGGATTGAGGGTTT+AGG - chr8_4:48334967-48334986 None:intergenic 40.0%
! TAAGGGTGTGTTTGGATTGA+GGG - chr8_4:48334973-48334992 None:intergenic 40.0%
! AAACAGACTAAGGGTGTGTT+TGG - chr8_4:48334981-48335000 None:intergenic 40.0%
AGCCTAATTCCAGTATGCAT+AGG + chr8_4:48335028-48335047 Msa1329620:intron 40.0%
GCCTAATTCCAGTATGCATA+GGG + chr8_4:48335029-48335048 Msa1329620:intron 40.0%
! CTGTAGTGGTACATCCTTTT+TGG + chr8_4:48335132-48335151 Msa1329620:intron 40.0%
! TGGTACATCCTTTTTGGATC+TGG + chr8_4:48335138-48335157 Msa1329620:intron 40.0%
! GGTACATCCTTTTTGGATCT+GGG + chr8_4:48335139-48335158 Msa1329620:intron 40.0%
CAACAAACAATGCATCAAGG+AGG - chr8_4:48335582-48335601 None:intergenic 40.0%
TAGAAGAGAACCGACATCTT+AGG + chr8_4:48335680-48335699 Msa1329620:three_prime_UTR 40.0%
! TGCCATTTGCTCTACTGATA+TGG + chr8_4:48335898-48335917 Msa1329620:three_prime_UTR 40.0%
GACAAAGCAAATTGCACGGA+AGG - chr8_4:48333170-48333189 None:intergenic 45.0%
CTGCGACAAAGCAAATTGCA+CGG - chr8_4:48333174-48333193 None:intergenic 45.0%
ATAGCAAAACGGCACAACGT+AGG + chr8_4:48333243-48333262 Msa1329620:CDS 45.0%
GTATCCGAACTAATCCTCGT+TGG + chr8_4:48333297-48333316 Msa1329620:CDS 45.0%
!! TCGACCAACGAGGATTAGTT+CGG - chr8_4:48333304-48333323 None:intergenic 45.0%
TGAAATCTCTTCGACCAACG+AGG - chr8_4:48333314-48333333 None:intergenic 45.0%
GAAGGTGGTTTGAAGAACCA+TGG - chr8_4:48333350-48333369 None:intergenic 45.0%
GAAAGTGACGAAATCGAAGG+TGG - chr8_4:48333365-48333384 None:intergenic 45.0%
TTGTTCAACCCACTTTCACC+CGG - chr8_4:48333830-48333849 None:intergenic 45.0%
!! TGATTTTGGGAGAATTGGCG+TGG + chr8_4:48334055-48334074 Msa1329620:intron 45.0%
CATTCAACAACCAACTTGGG+AGG + chr8_4:48334090-48334109 Msa1329620:intron 45.0%
GTAATCCTTGCGAGATCAGT+TGG + chr8_4:48334161-48334180 Msa1329620:intron 45.0%
TTTGCCAGTGGATGTAACAG+CGG + chr8_4:48334341-48334360 Msa1329620:intron 45.0%
! TTTTCCGCTGTTACATCCAC+TGG - chr8_4:48334348-48334367 None:intergenic 45.0%
TAGTCATGGGTGTGTTTGGA+TGG + chr8_4:48334755-48334774 Msa1329620:intron 45.0%
ATGGGTGTGTTTGGATGGAA+GGG + chr8_4:48334760-48334779 Msa1329620:intron 45.0%
TTGGATTGAGGGTTTAGGAG+GGG - chr8_4:48334962-48334981 None:intergenic 45.0%
GTTTGGATTGAGGGTTTAGG+AGG - chr8_4:48334964-48334983 None:intergenic 45.0%
! CTAAGGGTGTGTTTGGATTG+AGG - chr8_4:48334974-48334993 None:intergenic 45.0%
GCCCTATGCATACTGGAATT+AGG - chr8_4:48335033-48335052 None:intergenic 45.0%
ATTCCAGTATGCATAGGGCT+TGG + chr8_4:48335034-48335053 Msa1329620:intron 45.0%
AAGGGAACCCAGATCCAAAA+AGG - chr8_4:48335149-48335168 None:intergenic 45.0%
!!! CTTTTTTTGTCAGTCTGGGC+AGG + chr8_4:48335376-48335395 Msa1329620:intron 45.0%
TTTGCTGAGAGATCTCGTGA+TGG + chr8_4:48335400-48335419 Msa1329620:CDS 45.0%
TTTCGTCGACCATGTTTCAC+AGG - chr8_4:48335451-48335470 None:intergenic 45.0%
TGGTCGACGAAATTACTGCA+CGG + chr8_4:48335459-48335478 Msa1329620:CDS 45.0%
GGGCTTAAATGCTTCAGTTG+CGG + chr8_4:48335657-48335676 Msa1329620:three_prime_UTR 45.0%
CAACAACTGTCACTAGTGAC+AGG + chr8_4:48335812-48335831 Msa1329620:three_prime_UTR 45.0%
!!! TATTTTTCATTTTTAAATTA+CGG + chr8_4:48334794-48334813 Msa1329620:intron 5.0%
ACACTCCTTCGTCGAAAGAC+CGG - chr8_4:48333526-48333545 None:intergenic 50.0%
GCAGTACCACACCCATATTG+CGG - chr8_4:48333576-48333595 None:intergenic 50.0%
TGTTCAACCCACTTTCACCC+GGG - chr8_4:48333829-48333848 None:intergenic 50.0%
CTTCACCAACTGATCTCGCA+AGG - chr8_4:48334169-48334188 None:intergenic 50.0%
! TGAGGGACACATTTTGCCAG+TGG + chr8_4:48334329-48334348 Msa1329620:intron 50.0%
CATGGGTGTGTTTGGATGGA+AGG + chr8_4:48334759-48334778 Msa1329620:intron 50.0%
GTGTGTTTGGATGGAAGGGA+GGG + chr8_4:48334764-48334783 Msa1329620:intron 50.0%
TGTGTTTGGATGGAAGGGAG+GGG + chr8_4:48334765-48334784 Msa1329620:intron 50.0%
TTGAGGGTTTAGGAGGGGAA+GGG - chr8_4:48334957-48334976 None:intergenic 50.0%
ATTGAGGGTTTAGGAGGGGA+AGG - chr8_4:48334958-48334977 None:intergenic 50.0%
CATCCAAGCCCTATGCATAC+TGG - chr8_4:48335040-48335059 None:intergenic 50.0%
GCAGAGAGACCTGTGAAACA+TGG + chr8_4:48335439-48335458 Msa1329620:CDS 50.0%
GGCTCTACGCTCTTCGATTA+CGG - chr8_4:48335497-48335516 None:intergenic 50.0%
ATTGGCGGCATTCTCTCTTC+TGG - chr8_4:48335518-48335537 None:intergenic 50.0%
AGAAGAGAGAATGCCGCCAA+TGG + chr8_4:48335517-48335536 Msa1329620:CDS 50.0%
CTGAGAACCCTTTCGCAACG+CGG + chr8_4:48333110-48333129 Msa1329620:five_prime_UTR 55.0%
GCACGAAGCGCAACAGCATT+CGG + chr8_4:48333273-48333292 Msa1329620:CDS 55.0%
AGGTTCCGGTCTTTCGACGA+AGG + chr8_4:48333518-48333537 Msa1329620:CDS 55.0%
TGGATACTCCCGGGTGAAAG+TGG + chr8_4:48333818-48333837 Msa1329620:intron 55.0%
GGATACTCCCGGGTGAAAGT+GGG + chr8_4:48333819-48333838 Msa1329620:intron 55.0%
GGTGTGTTTGGATGGAAGGG+AGG + chr8_4:48334763-48334782 Msa1329620:intron 55.0%
TTTGGATGGAAGGGAGGGGA+GGG + chr8_4:48334769-48334788 Msa1329620:intron 55.0%
ACGGCTACCGCGTTGCGAAA+GGG - chr8_4:48333120-48333139 None:intergenic 60.0%
AACGGCTACCGCGTTGCGAA+AGG - chr8_4:48333121-48333140 None:intergenic 60.0%
GTTTGGATGGAAGGGAGGGG+AGG + chr8_4:48334768-48334787 Msa1329620:intron 60.0%
GGTTTAGGAGGGGAAGGGAG+GGG - chr8_4:48334952-48334971 None:intergenic 60.0%
GGGTTTAGGAGGGGAAGGGA+GGG - chr8_4:48334953-48334972 None:intergenic 60.0%
AGGGTTTAGGAGGGGAAGGG+AGG - chr8_4:48334954-48334973 None:intergenic 60.0%
TAGGAGGGGAAGGGAGGGGA+GGG - chr8_4:48334948-48334967 None:intergenic 65.0%
TTAGGAGGGGAAGGGAGGGG+AGG - chr8_4:48334949-48334968 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 48333104 48335929 48333104 ID=Msa1329620;Name=Msa1329620
chr8_4 mRNA 48333104 48335929 48333104 ID=Msa1329620-mRNA-1;Parent=Msa1329620;Name=Msa1329620-mRNA-1;_AED=0.00;_eAED=0.00;_QI=106|1|1|1|1|1|2|320|218
chr8_4 exon 48333104 48333645 48333104 ID=Msa1329620-mRNA-1:exon:13871;Parent=Msa1329620-mRNA-1
chr8_4 exon 48335389 48335929 48335389 ID=Msa1329620-mRNA-1:exon:13872;Parent=Msa1329620-mRNA-1
chr8_4 five_prime_UTR 48333104 48333209 48333104 ID=Msa1329620-mRNA-1:five_prime_utr;Parent=Msa1329620-mRNA-1
chr8_4 CDS 48333210 48333645 48333210 ID=Msa1329620-mRNA-1:cds;Parent=Msa1329620-mRNA-1
chr8_4 CDS 48335389 48335609 48335389 ID=Msa1329620-mRNA-1:cds;Parent=Msa1329620-mRNA-1
chr8_4 three_prime_UTR 48335610 48335929 48335610 ID=Msa1329620-mRNA-1:three_prime_utr;Parent=Msa1329620-mRNA-1
Gene Sequence

>Msa1329620

ATGGTGTTTGAAAGCTATGTAGTTGTACACAACATAGCAAAACGGCACAACGTAGGAACACTAGCACGAAGCGCAACAGCATTCGGCGTATCCGAACTAATCCTCGTTGGTCGAAGAGATTTCAACAGTTTCGGTAACCATGGTTCTTCAAACCACCTTCGATTTCGTCACTTTCACTCACTTCAAGACGCTAAACAATTTCTCAAAGACAAAGATTGTGACATTTGCGGCGTCGAGATCACACACGATGCTCTTCCTGTTAATCAACATCCTTTTAAGAGAAGTACTGCTTTCTTGCTTGGTAATGAAGGTTCCGGTCTTTCGACGAAGGAGTGTGAAATTTGTGATTTTTTTGTTTATATTCCGCAATATGGGTGTGGTACTGCTTCGTTGAATGTTACTGTTGCTGCTTCTATTGTGCTTCATCAATTTGGAGTCTGGGCAGGTTTTGCTGAGAGATCTCGTGATGGAAATAAATTTGTTGTAGCAGAGAGACCTGTGAAACATGGTCGACGAAATTACTGCACGGAAACAGATGATTCCGTAATCGAAGAGCGTAGAGCCAGAAGAGAGAATGCCGCCAATGGTTTCTTTGAAGAAGCTGAGAGTAGCAATTCATCTTCAAACCTCCTTGATGCATTGTTTGTTGATGGTTGA
Protein sequence

>Msa1329620

MVFESYVVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNSFGNHGSSNHLRFRHFHSLQDAKQFLKDKDCDICGVEITHDALPVNQHPFKRSTAFLLGNEGSGLSTKECEICDFFVYIPQYGCGTASLNVTVAASIVLHQFGVWAGFAERSRDGNKFVVAERPVKHGRRNYCTETDDSVIEERRARRENAANGFFEEAESSNSSSNLLDALFVDG*