AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1216910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1216910 MtrunA17_Chr4g0066451 98.165 218 4 0 1 218 1 218 1.06e-160 442
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1216910 AT4G15520.1 76.147 218 49 2 3 217 4 221 5.41e-122 345

Find 58 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTATTGTGCTTCATCAATT+TGG 0.178884 8_1:+79091439 Msa1216910:CDS
CTTGATGCATTGTTTGTTGA+TGG 0.220225 8_1:+79093399 Msa1216910:CDS
CAATTGACAGATTGTAGTTT+AGG 0.238093 8_1:+79093452 Msa1216910:three_prime_UTR
TTAAAAGGATGTTGATTAAC+AGG 0.260098 8_1:-79091283 None:intergenic
AAAGCAGTACTTCTCTTAAA+AGG 0.284242 8_1:-79091298 None:intergenic
TTTGTTTATATTCCGCAATA+TGG 0.296529 8_1:+79091379 Msa1216910:CDS
TGCCATTTGCTCTACTGATA+TGG 0.299194 8_1:+79093714 Msa1216910:three_prime_UTR
CTTAGGTATCTAATGTTGTT+TGG 0.325239 8_1:+79093513 Msa1216910:three_prime_UTR
CATTGTTTGTTGATGGTTGA+TGG 0.330834 8_1:+79093406 Msa1216910:CDS
AATTGACAGATTGTAGTTTA+GGG 0.333797 8_1:+79093453 Msa1216910:three_prime_UTR
CGAAGAGATTTCAACAGTTT+CGG 0.342769 8_1:+79091139 Msa1216910:CDS
AGAAGTACTGCTTTCTTGCT+TGG 0.377151 8_1:+79091307 Msa1216910:CDS
TAGAAGAGAACCGACATCTT+AGG 0.393257 8_1:+79093496 Msa1216910:three_prime_UTR
ATTGGCGGCATTCTCTCTTC+TGG 0.397261 8_1:-79093331 None:intergenic
AGAAGATTTCTACTGTCATT+AGG 0.401312 8_1:+79093578 Msa1216910:three_prime_UTR
CAGAGTTGATACTAACAAAA+TGG 0.406161 8_1:+79091009 Msa1216910:exon
GCACGAAGCGCAACAGCATT+CGG 0.421833 8_1:+79091091 Msa1216910:CDS
GCTTTCTTGCTTGGTAATGA+AGG 0.426363 8_1:+79091316 Msa1216910:CDS
TTGCTTGGTAATGAAGGTTC+CGG 0.428513 8_1:+79091322 Msa1216910:CDS
AACCATATCAGTAGAGCAAA+TGG 0.443015 8_1:-79093716 None:intergenic
TTGTTTATATTCCGCAATAT+GGG 0.446663 8_1:+79091380 Msa1216910:CDS
TCGACCAACGAGGATTAGTT+CGG 0.466321 8_1:-79091119 None:intergenic
ATATATACAGAGGCATTAGC+TGG 0.474340 8_1:+79093551 Msa1216910:three_prime_UTR
GACAAAGATTGTGACATTTG+CGG 0.478875 8_1:+79091235 Msa1216910:CDS
ACACTCCTTCGTCGAAAGAC+CGG 0.479365 8_1:-79091341 None:intergenic
ATTGTGCTTCATCAATTTGG+AGG 0.481305 8_1:+79091442 Msa1216910:CDS
GCAAATGGCAGTATAATAGT+TGG 0.481426 8_1:-79093701 None:intergenic
TATTTGTTGCAGAGAGAAAA+CGG 0.487646 8_1:-79090954 None:intergenic
GGCTCTACGCTCTTCGATGA+CGG 0.492169 8_1:-79093310 None:intergenic
AACGGCTACCGCGTTGCGAA+AGG 0.502306 8_1:-79090936 None:intergenic
AGCTTCTTCAAAGAAACCAT+TGG 0.515730 8_1:-79093349 None:intergenic
TTTCGTCGACCATGTTTCAC+AGG 0.524298 8_1:-79093264 None:intergenic
ACGGCTACCGCGTTGCGAAA+GGG 0.531792 8_1:-79090935 None:intergenic
TATATTCCGCAATATGGGTG+TGG 0.552488 8_1:+79091385 Msa1216910:CDS
TTCTTCAAAGAAACCATTGG+CGG 0.556928 8_1:-79093346 None:intergenic
CAACAACTGTCACTAGTGAC+AGG 0.557401 8_1:+79093628 Msa1216910:three_prime_UTR
GACAAAGCAAATTGCACGGA+AGG 0.567099 8_1:-79090985 None:intergenic
TTCAACAGTTTCGGTAACCA+TGG 0.567269 8_1:+79091148 Msa1216910:CDS
GGGCTTAAATGCTTCAGTTG+CGG 0.602530 8_1:+79093473 Msa1216910:three_prime_UTR
AGAAGAGAGAATGCCGCCAA+TGG 0.603778 8_1:+79093333 Msa1216910:CDS
ACATTAGATACCTAAGATGT+CGG 0.604714 8_1:-79093506 None:intergenic
TTGTACACAACATAGCAAAA+CGG 0.606644 8_1:+79091050 Msa1216910:CDS
AGGTTCCGGTCTTTCGACGA+AGG 0.609762 8_1:+79091336 Msa1216910:CDS
CATCAACAAACAATGCATCA+AGG 0.612565 8_1:-79093398 None:intergenic
GCAGAGAGACCTGTGAAACA+TGG 0.616817 8_1:+79093255 Msa1216910:CDS
TATATACAGAGGCATTAGCT+GGG 0.617803 8_1:+79093552 Msa1216910:three_prime_UTR
GTATCCGAACTAATCCTCGT+TGG 0.630687 8_1:+79091115 Msa1216910:CDS
GAAGGTGGTTTGAAGAACCA+TGG 0.643878 8_1:-79091165 None:intergenic
CTGCGACAAAGCAAATTGCA+CGG 0.652456 8_1:-79090989 None:intergenic
GAAAGTGACGAAATCGAAGG+TGG 0.672321 8_1:-79091180 None:intergenic
TGAAATCTCTTCGACCAACG+AGG 0.674642 8_1:-79091129 None:intergenic
AGTGAAAGTGACGAAATCGA+AGG 0.683890 8_1:-79091183 None:intergenic
TGGTCGACGAAATTACTGCA+CGG 0.684868 8_1:+79093275 Msa1216910:CDS
ACTGACTATTATATATACAG+AGG 0.686318 8_1:+79093541 Msa1216910:three_prime_UTR
TTTGCTGAGAGATCTCGTGA+TGG 0.690113 8_1:+79093216 Msa1216910:CDS
GCAGTACCACACCCATATTG+CGG 0.695525 8_1:-79091391 None:intergenic
ATAGCAAAACGGCACAACGT+AGG 0.696815 8_1:+79091061 Msa1216910:CDS
CAACAAACAATGCATCAAGG+AGG 0.748014 8_1:-79093395 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTGTATGTAAAAAAA+AGG - chr8_1:79092245-79092264 None:intergenic 10.0%
!!! AATAAATTTGCATAATTTTT+TGG - chr8_1:79092421-79092440 None:intergenic 10.0%
!!! AAATTTGCACTTAGATTTTT+GGG + chr8_1:79091829-79091848 Msa1216910:intron 20.0%
!!! ATTCTTTGAAATCTTGATTT+TGG + chr8_1:79091859-79091878 Msa1216910:intron 20.0%
!!! TTCTTTGAAATCTTGATTTT+GGG + chr8_1:79091860-79091879 Msa1216910:intron 20.0%
!! GGAAATGTTATTGATATATT+GGG - chr8_1:79092049-79092068 None:intergenic 20.0%
!! AGGAAATGTTATTGATATAT+TGG - chr8_1:79092050-79092069 None:intergenic 20.0%
!!! TTTTTGGTTACATTTAGATT+TGG + chr8_1:79093003-79093022 Msa1216910:intron 20.0%
!! TTAAAAGGATGTTGATTAAC+AGG - chr8_1:79091286-79091305 None:intergenic 25.0%
! TTTGTTTATATTCCGCAATA+TGG + chr8_1:79091379-79091398 Msa1216910:CDS 25.0%
! TTGTTTATATTCCGCAATAT+GGG + chr8_1:79091380-79091399 Msa1216910:CDS 25.0%
!!! GAAATTGATTTTGGTACATT+TGG - chr8_1:79091768-79091787 None:intergenic 25.0%
!!! TGATGACTTGAAATTGATTT+TGG - chr8_1:79091777-79091796 None:intergenic 25.0%
!!! GCAAATTTGAATTCACTTTT+GGG - chr8_1:79091817-79091836 None:intergenic 25.0%
!! TGCAAATTTGAATTCACTTT+TGG - chr8_1:79091818-79091837 None:intergenic 25.0%
!! CAAATTTGCACTTAGATTTT+TGG + chr8_1:79091828-79091847 Msa1216910:intron 25.0%
!! CTGAAAAGGTATATAAACAT+GGG - chr8_1:79091940-79091959 None:intergenic 25.0%
!! ACTGAAAAGGTATATAAACA+TGG - chr8_1:79091941-79091960 None:intergenic 25.0%
!! CTTAATATTGAAGGACATTT+TGG + chr8_1:79092091-79092110 Msa1216910:intron 25.0%
!! TATGTAAAAAAAAGGCATGT+TGG - chr8_1:79092237-79092256 None:intergenic 25.0%
!!! TTTTTTGGAAGTGCTATAAT+AGG - chr8_1:79092406-79092425 None:intergenic 25.0%
! ACTACAATAAGACATTAACA+AGG + chr8_1:79092449-79092468 Msa1216910:intron 25.0%
!! AAGTTTTTCACTAAAAGAAC+TGG - chr8_1:79092505-79092524 None:intergenic 25.0%
!! GTTCTTTTAGTGAAAAACTT+TGG + chr8_1:79092505-79092524 Msa1216910:intron 25.0%
!!! ATCATGAAAGTTTTAGTCAT+GGG + chr8_1:79092558-79092577 Msa1216910:intron 25.0%
! GAAAAACATGTTATATAGTC+TGG + chr8_1:79092717-79092736 Msa1216910:intron 25.0%
!!! TTGTTTATGTGCTCATTTTT+TGG + chr8_1:79092987-79093006 Msa1216910:intron 25.0%
! AACAAAACCAATATACAGTT+GGG - chr8_1:79093040-79093059 None:intergenic 25.0%
! TTAAGTAAAAAGATGCAGTT+GGG - chr8_1:79093096-79093115 None:intergenic 25.0%
! ATTAAGTAAAAAGATGCAGT+TGG - chr8_1:79093097-79093116 None:intergenic 25.0%
! AACCAATATCATAATGAATG+AGG - chr8_1:79093123-79093142 None:intergenic 25.0%
! AATTGACAGATTGTAGTTTA+GGG + chr8_1:79093453-79093472 Msa1216910:three_prime_UTR 25.0%
! ACTGACTATTATATATACAG+AGG + chr8_1:79093541-79093560 Msa1216910:three_prime_UTR 25.0%
! TATTTGTTGCAGAGAGAAAA+CGG - chr8_1:79090957-79090976 None:intergenic 30.0%
!! CAGAGTTGATACTAACAAAA+TGG + chr8_1:79091009-79091028 Msa1216910:exon 30.0%
TTGTACACAACATAGCAAAA+CGG + chr8_1:79091050-79091069 Msa1216910:CDS 30.0%
AAAGCAGTACTTCTCTTAAA+AGG - chr8_1:79091301-79091320 None:intergenic 30.0%
TCTATTGTGCTTCATCAATT+TGG + chr8_1:79091439-79091458 Msa1216910:CDS 30.0%
! GTTGTGGTGATATTTTATGT+AGG + chr8_1:79091536-79091555 Msa1216910:intron 30.0%
!! TAACCTGCAAAAGTTGATTT+TGG + chr8_1:79091566-79091585 Msa1216910:intron 30.0%
AAGAGAAAACGATTTATGTC+TGG + chr8_1:79091616-79091635 Msa1216910:intron 30.0%
!! AATCTTGATTTTGGGAGAAT+TGG + chr8_1:79091868-79091887 Msa1216910:intron 30.0%
TAACATTCAACAACCAACTT+GGG + chr8_1:79091905-79091924 Msa1216910:intron 30.0%
!! ATCAGTTGGTGAAGAAAATA+TGG + chr8_1:79091991-79092010 Msa1216910:intron 30.0%
!! GTGTTGCTTCTTAATATTGA+AGG + chr8_1:79092082-79092101 Msa1216910:intron 30.0%
ATGAGGTGAAAAATTCATTG+AGG + chr8_1:79092127-79092146 Msa1216910:intron 30.0%
TGAGGTGAAAAATTCATTGA+GGG + chr8_1:79092128-79092147 Msa1216910:intron 30.0%
! TATGTAGCATATAGTGTTGA+AGG - chr8_1:79092300-79092319 None:intergenic 30.0%
! CTTTTAGTGAAAAACTTTGG+TGG + chr8_1:79092508-79092527 Msa1216910:intron 30.0%
!! CATCATGAAAGTTTTAGTCA+TGG + chr8_1:79092557-79092576 Msa1216910:intron 30.0%
! AACGTAAATTAGTCTGTAAC+TGG + chr8_1:79092894-79092913 Msa1216910:intron 30.0%
GTCATATGTTATCACTTACA+AGG - chr8_1:79092926-79092945 None:intergenic 30.0%
ATAACATATGACTACTGTAG+TGG + chr8_1:79092934-79092953 Msa1216910:intron 30.0%
ATGAGCACATAAACAACAAA+GGG - chr8_1:79092983-79093002 None:intergenic 30.0%
AATGAGCACATAAACAACAA+AGG - chr8_1:79092984-79093003 None:intergenic 30.0%
TCATATTCCCAACTGTATAT+TGG + chr8_1:79093030-79093049 Msa1216910:intron 30.0%
CAACAAAACCAATATACAGT+TGG - chr8_1:79093041-79093060 None:intergenic 30.0%
CACCTCATTCATTATGATAT+TGG + chr8_1:79093118-79093137 Msa1216910:intron 30.0%
!!! TTCACTTTTTTTGTCAGTCT+GGG + chr8_1:79093188-79093207 Msa1216910:intron 30.0%
CAATTGACAGATTGTAGTTT+AGG + chr8_1:79093452-79093471 Msa1216910:three_prime_UTR 30.0%
ACATTAGATACCTAAGATGT+CGG - chr8_1:79093509-79093528 None:intergenic 30.0%
CTTAGGTATCTAATGTTGTT+TGG + chr8_1:79093513-79093532 Msa1216910:three_prime_UTR 30.0%
AGAAGATTTCTACTGTCATT+AGG + chr8_1:79093578-79093597 Msa1216910:three_prime_UTR 30.0%
CGAAGAGATTTCAACAGTTT+CGG + chr8_1:79091139-79091158 Msa1216910:CDS 35.0%
GACAAAGATTGTGACATTTG+CGG + chr8_1:79091235-79091254 Msa1216910:CDS 35.0%
ATTGTGCTTCATCAATTTGG+AGG + chr8_1:79091442-79091461 Msa1216910:CDS 35.0%
! CAACCAAAATCAACTTTTGC+AGG - chr8_1:79091572-79091591 None:intergenic 35.0%
!! TTTGAGTGCTGAATCACATT+TGG + chr8_1:79091672-79091691 Msa1216910:intron 35.0%
! CAAGTCATCAGAATCACTTT+TGG + chr8_1:79091787-79091806 Msa1216910:intron 35.0%
GTAACATTCAACAACCAACT+TGG + chr8_1:79091904-79091923 Msa1216910:intron 35.0%
AGGATTCGTGAAAACTGAAA+AGG - chr8_1:79091954-79091973 None:intergenic 35.0%
TGAAGAAAATATGGAGTTGC+TGG + chr8_1:79092000-79092019 Msa1216910:intron 35.0%
AGCAACACTCACTCATAAAA+AGG - chr8_1:79092070-79092089 None:intergenic 35.0%
!! TATTGAAGGACATTTTGGTG+TGG + chr8_1:79092096-79092115 Msa1216910:intron 35.0%
!! ATTGAAGGACATTTTGGTGT+GGG + chr8_1:79092097-79092116 Msa1216910:intron 35.0%
GACTTATTTCAACTGCTATG+CGG + chr8_1:79092266-79092285 Msa1216910:intron 35.0%
TTAGTGAAAAACTTTGGTGG+TGG + chr8_1:79092511-79092530 Msa1216910:intron 35.0%
GTGTCAAAGAAACAGACTAA+GGG - chr8_1:79092806-79092825 None:intergenic 35.0%
AGTGTCAAAGAAACAGACTA+AGG - chr8_1:79092807-79092826 None:intergenic 35.0%
!!! GTTCACTTTTTTTGTCAGTC+TGG + chr8_1:79093187-79093206 Msa1216910:intron 35.0%
TTCTTCAAAGAAACCATTGG+CGG - chr8_1:79093349-79093368 None:intergenic 35.0%
AGCTTCTTCAAAGAAACCAT+TGG - chr8_1:79093352-79093371 None:intergenic 35.0%
CATCAACAAACAATGCATCA+AGG - chr8_1:79093401-79093420 None:intergenic 35.0%
! CTTGATGCATTGTTTGTTGA+TGG + chr8_1:79093399-79093418 Msa1216910:CDS 35.0%
!! CATTGTTTGTTGATGGTTGA+TGG + chr8_1:79093406-79093425 Msa1216910:CDS 35.0%
!! ATATATACAGAGGCATTAGC+TGG + chr8_1:79093551-79093570 Msa1216910:three_prime_UTR 35.0%
!! TATATACAGAGGCATTAGCT+GGG + chr8_1:79093552-79093571 Msa1216910:three_prime_UTR 35.0%
GCAAATGGCAGTATAATAGT+TGG - chr8_1:79093704-79093723 None:intergenic 35.0%
AACCATATCAGTAGAGCAAA+TGG - chr8_1:79093719-79093738 None:intergenic 35.0%
TTCAACAGTTTCGGTAACCA+TGG + chr8_1:79091148-79091167 Msa1216910:CDS 40.0%
AGTGAAAGTGACGAAATCGA+AGG - chr8_1:79091186-79091205 None:intergenic 40.0%
!! AGAAGTACTGCTTTCTTGCT+TGG + chr8_1:79091307-79091326 Msa1216910:CDS 40.0%
!! GCTTTCTTGCTTGGTAATGA+AGG + chr8_1:79091316-79091335 Msa1216910:CDS 40.0%
!! TTGCTTGGTAATGAAGGTTC+CGG + chr8_1:79091322-79091341 Msa1216910:CDS 40.0%
TATATTCCGCAATATGGGTG+TGG + chr8_1:79091385-79091404 Msa1216910:CDS 40.0%
TTCGCGATAAAAGAACGTTG+TGG + chr8_1:79091520-79091539 Msa1216910:intron 40.0%
GATTTATGTCTGGATACTCC+CGG + chr8_1:79091626-79091645 Msa1216910:intron 40.0%
ATTTATGTCTGGATACTCCC+GGG + chr8_1:79091627-79091646 Msa1216910:intron 40.0%
GATCTCGCAAGGATTACTTA+AGG - chr8_1:79091974-79091993 None:intergenic 40.0%
TTGGTGTGGGATTGAATATG+AGG + chr8_1:79092110-79092129 Msa1216910:intron 40.0%
AGCGGAAAAGAGAGAATTTC+AGG + chr8_1:79092175-79092194 Msa1216910:intron 40.0%
GCGGAAAAGAGAGAATTTCA+GGG + chr8_1:79092176-79092195 Msa1216910:intron 40.0%
! AGCATATAGTGTTGAAGGTG+CGG - chr8_1:79092295-79092314 None:intergenic 40.0%
TCCGCCATTAATAACACTGA+TGG + chr8_1:79092351-79092370 Msa1216910:intron 40.0%
! CCCATCAGTGTTATTAATGG+CGG - chr8_1:79092355-79092374 None:intergenic 40.0%
CCGCCATTAATAACACTGAT+GGG + chr8_1:79092352-79092371 Msa1216910:intron 40.0%
! GTGCCCATCAGTGTTATTAA+TGG - chr8_1:79092358-79092377 None:intergenic 40.0%
CACTAAAAGAACTGGCTACA+AGG - chr8_1:79092497-79092516 None:intergenic 40.0%
!! GTTTTAGTCATGGGTGTGTT+TGG + chr8_1:79092567-79092586 Msa1216910:intron 40.0%
TTTGGATTGAGGGTTTAGGA+GGG - chr8_1:79092779-79092798 None:intergenic 40.0%
TGTGTTTGGATTGAGGGTTT+AGG - chr8_1:79092783-79092802 None:intergenic 40.0%
! TAAGGGTGTGTTTGGATTGA+GGG - chr8_1:79092789-79092808 None:intergenic 40.0%
! AAACAGACTAAGGGTGTGTT+TGG - chr8_1:79092797-79092816 None:intergenic 40.0%
AGCCTAATTCCAGTATGCAT+AGG + chr8_1:79092844-79092863 Msa1216910:intron 40.0%
GCCTAATTCCAGTATGCATA+GGG + chr8_1:79092845-79092864 Msa1216910:intron 40.0%
! CTGTAGTGGTACATCCTTTT+TGG + chr8_1:79092948-79092967 Msa1216910:intron 40.0%
! TGGTACATCCTTTTTGGATC+TGG + chr8_1:79092954-79092973 Msa1216910:intron 40.0%
! GGTACATCCTTTTTGGATCT+GGG + chr8_1:79092955-79092974 Msa1216910:intron 40.0%
CAACAAACAATGCATCAAGG+AGG - chr8_1:79093398-79093417 None:intergenic 40.0%
TAGAAGAGAACCGACATCTT+AGG + chr8_1:79093496-79093515 Msa1216910:three_prime_UTR 40.0%
! TGCCATTTGCTCTACTGATA+TGG + chr8_1:79093714-79093733 Msa1216910:three_prime_UTR 40.0%
GACAAAGCAAATTGCACGGA+AGG - chr8_1:79090988-79091007 None:intergenic 45.0%
CTGCGACAAAGCAAATTGCA+CGG - chr8_1:79090992-79091011 None:intergenic 45.0%
ATAGCAAAACGGCACAACGT+AGG + chr8_1:79091061-79091080 Msa1216910:CDS 45.0%
GTATCCGAACTAATCCTCGT+TGG + chr8_1:79091115-79091134 Msa1216910:CDS 45.0%
!! TCGACCAACGAGGATTAGTT+CGG - chr8_1:79091122-79091141 None:intergenic 45.0%
TGAAATCTCTTCGACCAACG+AGG - chr8_1:79091132-79091151 None:intergenic 45.0%
GAAGGTGGTTTGAAGAACCA+TGG - chr8_1:79091168-79091187 None:intergenic 45.0%
GAAAGTGACGAAATCGAAGG+TGG - chr8_1:79091183-79091202 None:intergenic 45.0%
TTGTTCAACCCACTTTCACC+CGG - chr8_1:79091648-79091667 None:intergenic 45.0%
!! TGATTTTGGGAGAATTGGCG+TGG + chr8_1:79091873-79091892 Msa1216910:intron 45.0%
CATTCAACAACCAACTTGGG+AGG + chr8_1:79091908-79091927 Msa1216910:intron 45.0%
TGGGATAAATCCTCCCAAGT+TGG - chr8_1:79091921-79091940 None:intergenic 45.0%
GTAATCCTTGCGAGATCAGT+TGG + chr8_1:79091977-79091996 Msa1216910:intron 45.0%
TTTGCCAGTGGATGTAACAG+CGG + chr8_1:79092157-79092176 Msa1216910:intron 45.0%
! TTTTCCGCTGTTACATCCAC+TGG - chr8_1:79092164-79092183 None:intergenic 45.0%
TAGTCATGGGTGTGTTTGGA+TGG + chr8_1:79092571-79092590 Msa1216910:intron 45.0%
ATGGGTGTGTTTGGATGGAA+GGG + chr8_1:79092576-79092595 Msa1216910:intron 45.0%
TTGGATTGAGGGTTTAGGAG+GGG - chr8_1:79092778-79092797 None:intergenic 45.0%
GTTTGGATTGAGGGTTTAGG+AGG - chr8_1:79092780-79092799 None:intergenic 45.0%
! CTAAGGGTGTGTTTGGATTG+AGG - chr8_1:79092790-79092809 None:intergenic 45.0%
GCCCTATGCATACTGGAATT+AGG - chr8_1:79092849-79092868 None:intergenic 45.0%
ATTCCAGTATGCATAGGGCT+TGG + chr8_1:79092850-79092869 Msa1216910:intron 45.0%
AAGGGAACCCAGATCCAAAA+AGG - chr8_1:79092965-79092984 None:intergenic 45.0%
!!! CTTTTTTTGTCAGTCTGGGC+AGG + chr8_1:79093192-79093211 Msa1216910:intron 45.0%
TTTGCTGAGAGATCTCGTGA+TGG + chr8_1:79093216-79093235 Msa1216910:CDS 45.0%
TTTCGTCGACCATGTTTCAC+AGG - chr8_1:79093267-79093286 None:intergenic 45.0%
TGGTCGACGAAATTACTGCA+CGG + chr8_1:79093275-79093294 Msa1216910:CDS 45.0%
GGGCTTAAATGCTTCAGTTG+CGG + chr8_1:79093473-79093492 Msa1216910:three_prime_UTR 45.0%
CAACAACTGTCACTAGTGAC+AGG + chr8_1:79093628-79093647 Msa1216910:three_prime_UTR 45.0%
ACACTCCTTCGTCGAAAGAC+CGG - chr8_1:79091344-79091363 None:intergenic 50.0%
GCAGTACCACACCCATATTG+CGG - chr8_1:79091394-79091413 None:intergenic 50.0%
TGTTCAACCCACTTTCACCC+GGG - chr8_1:79091647-79091666 None:intergenic 50.0%
CTTCACCAACTGATCTCGCA+AGG - chr8_1:79091985-79092004 None:intergenic 50.0%
! TGAGGGACACATTTTGCCAG+TGG + chr8_1:79092145-79092164 Msa1216910:intron 50.0%
CATGGGTGTGTTTGGATGGA+AGG + chr8_1:79092575-79092594 Msa1216910:intron 50.0%
GTGTGTTTGGATGGAAGGGA+GGG + chr8_1:79092580-79092599 Msa1216910:intron 50.0%
TGTGTTTGGATGGAAGGGAG+GGG + chr8_1:79092581-79092600 Msa1216910:intron 50.0%
TTGAGGGTTTAGGAGGGGAA+GGG - chr8_1:79092773-79092792 None:intergenic 50.0%
ATTGAGGGTTTAGGAGGGGA+AGG - chr8_1:79092774-79092793 None:intergenic 50.0%
CATCCAAGCCCTATGCATAC+TGG - chr8_1:79092856-79092875 None:intergenic 50.0%
GCAGAGAGACCTGTGAAACA+TGG + chr8_1:79093255-79093274 Msa1216910:CDS 50.0%
ATTGGCGGCATTCTCTCTTC+TGG - chr8_1:79093334-79093353 None:intergenic 50.0%
AGAAGAGAGAATGCCGCCAA+TGG + chr8_1:79093333-79093352 Msa1216910:CDS 50.0%
CTGAGAACCCTTTCGCAACG+CGG + chr8_1:79090928-79090947 Msa1216910:five_prime_UTR 55.0%
GCACGAAGCGCAACAGCATT+CGG + chr8_1:79091091-79091110 Msa1216910:CDS 55.0%
AGGTTCCGGTCTTTCGACGA+AGG + chr8_1:79091336-79091355 Msa1216910:CDS 55.0%
TGGATACTCCCGGGTGAAAG+TGG + chr8_1:79091636-79091655 Msa1216910:intron 55.0%
GGATACTCCCGGGTGAAAGT+GGG + chr8_1:79091637-79091656 Msa1216910:intron 55.0%
GGTGTGTTTGGATGGAAGGG+AGG + chr8_1:79092579-79092598 Msa1216910:intron 55.0%
TTTGGATGGAAGGGAGGGGA+GGG + chr8_1:79092585-79092604 Msa1216910:intron 55.0%
GGCTCTACGCTCTTCGATGA+CGG - chr8_1:79093313-79093332 None:intergenic 55.0%
ACGGCTACCGCGTTGCGAAA+GGG - chr8_1:79090938-79090957 None:intergenic 60.0%
AACGGCTACCGCGTTGCGAA+AGG - chr8_1:79090939-79090958 None:intergenic 60.0%
GTTTGGATGGAAGGGAGGGG+AGG + chr8_1:79092584-79092603 Msa1216910:intron 60.0%
GGTTTAGGAGGGGAAGGGAG+GGG - chr8_1:79092768-79092787 None:intergenic 60.0%
GGGTTTAGGAGGGGAAGGGA+GGG - chr8_1:79092769-79092788 None:intergenic 60.0%
AGGGTTTAGGAGGGGAAGGG+AGG - chr8_1:79092770-79092789 None:intergenic 60.0%
TAGGAGGGGAAGGGAGGGGA+GGG - chr8_1:79092764-79092783 None:intergenic 65.0%
TTAGGAGGGGAAGGGAGGGG+AGG - chr8_1:79092765-79092784 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 79090922 79093745 79090922 ID=Msa1216910;Name=Msa1216910
chr8_1 mRNA 79090922 79093745 79090922 ID=Msa1216910-mRNA-1;Parent=Msa1216910;Name=Msa1216910-mRNA-1;_AED=0.00;_eAED=0.00;_QI=106|1|1|1|1|1|2|320|218
chr8_1 exon 79090922 79091463 79090922 ID=Msa1216910-mRNA-1:exon:27410;Parent=Msa1216910-mRNA-1
chr8_1 exon 79093205 79093745 79093205 ID=Msa1216910-mRNA-1:exon:27411;Parent=Msa1216910-mRNA-1
chr8_1 five_prime_UTR 79090922 79091027 79090922 ID=Msa1216910-mRNA-1:five_prime_utr;Parent=Msa1216910-mRNA-1
chr8_1 CDS 79091028 79091463 79091028 ID=Msa1216910-mRNA-1:cds;Parent=Msa1216910-mRNA-1
chr8_1 CDS 79093205 79093425 79093205 ID=Msa1216910-mRNA-1:cds;Parent=Msa1216910-mRNA-1
chr8_1 three_prime_UTR 79093426 79093745 79093426 ID=Msa1216910-mRNA-1:three_prime_utr;Parent=Msa1216910-mRNA-1
Gene Sequence

>Msa1216910

ATGGTGTTTGAAAGCTATGTAGTTGTACACAACATAGCAAAACGGCACAACGTAGGAACACTAGCACGAAGCGCAACAGCATTCGGCGTATCCGAACTAATCCTCGTTGGTCGAAGAGATTTCAACAGTTTCGGTAACCATGGTTCTTCAAACCACCTTCGATTTCGTCACTTTCACTCACTTCAAGACGCTAAACAATTTCTCAAAGACAAAGATTGTGACATTTGCGGCGTCGAGATCACACACGATGCTCTTCCTGTTAATCAACATCCTTTTAAGAGAAGTACTGCTTTCTTGCTTGGTAATGAAGGTTCCGGTCTTTCGACGAAGGAGTGTGAAATTTGTGATTTTTTTGTTTATATTCCGCAATATGGGTGTGGTACTGCTTCGTTGAATGTTACTGTTGCTGCTTCTATTGTGCTTCATCAATTTGGAGTCTGGGCAGGTTTTGCTGAGAGATCTCGTGATGGAAATAAATTTGTTGTAGCAGAGAGACCTGTGAAACATGGTCGACGAAATTACTGCACGGAAACAGATGATTCCGTCATCGAAGAGCGTAGAGCCAGAAGAGAGAATGCCGCCAATGGTTTCTTTGAAGAAGCTGAGAGTAGCAATTCATCTTCAAACCTCCTTGATGCATTGTTTGTTGATGGTTGA
Protein sequence

>Msa1216910

MVFESYVVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNSFGNHGSSNHLRFRHFHSLQDAKQFLKDKDCDICGVEITHDALPVNQHPFKRSTAFLLGNEGSGLSTKECEICDFFVYIPQYGCGTASLNVTVAASIVLHQFGVWAGFAERSRDGNKFVVAERPVKHGRRNYCTETDDSVIEERRARRENAANGFFEEAESSNSSSNLLDALFVDG*