Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1331020 | A0A396IBL7 | 96.296 | 54 | 2 | 0 | 1 | 54 | 113 | 166 | 1.53e-28 | 110 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1204550 | Msa1331020 | 0.928849 | 1.612423e-92 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1331020 | MtrunA17_Chr4g0053671 | 96.296 | 54 | 2 | 0 | 1 | 54 | 113 | 166 | 2.94e-32 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACAATAGAATATAAACTAA+TGG | 0.278862 | 8_4:-50349668 | None:intergenic |
| ACAATAGAATATAAACTAAT+GGG | 0.357341 | 8_4:-50349667 | None:intergenic |
| AAAGAGGTTAACTTGGATAG+TGG | 0.467598 | 8_4:+50349719 | Msa1331020:CDS |
| TGTTATACTCTATTTCTAAG+AGG | 0.486122 | 8_4:-50349643 | None:intergenic |
| CCGTAGGAAAGAGGTTAACT+TGG | 0.506970 | 8_4:+50349712 | Msa1331020:CDS |
| CCAAGTTAACCTCTTTCCTA+CGG | 0.567694 | 8_4:-50349712 | None:intergenic |
| AACAGCAGACCGTAGGAAAG+AGG | 0.579266 | 8_4:+50349703 | Msa1331020:CDS |
| GCAATACAACAGCAGACCGT+AGG | 0.615669 | 8_4:+50349696 | Msa1331020:intron |
| CATGGTTACAAACAGTGAAG+TGG | 0.661662 | 8_4:+50349526 | None:intergenic |
| TTCGCACATCAAGTTGACAA+TGG | 0.665212 | 8_4:+50349557 | Msa1331020:CDS |
| GGTGTTGTACTCCACAATGT+CGG | 0.682313 | 8_4:+50349578 | Msa1331020:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAATAGAATATAAACTAAT+GGG | - | chr8_4:50349670-50349689 | None:intergenic | 15.0% |
| !! | TACAATAGAATATAAACTAA+TGG | - | chr8_4:50349671-50349690 | None:intergenic | 15.0% |
| !!! | TGTTATACTCTATTTCTAAG+AGG | - | chr8_4:50349646-50349665 | None:intergenic | 25.0% |
| ! | AAAAACTTGTACCGACATTG+TGG | - | chr8_4:50349592-50349611 | None:intergenic | 35.0% |
| ! | TCGGTACAAGTTTTTGTGAT+GGG | + | chr8_4:50349597-50349616 | Msa1331020:CDS | 35.0% |
| ! | AAAGAGGTTAACTTGGATAG+TGG | + | chr8_4:50349719-50349738 | Msa1331020:CDS | 35.0% |
| !! | CTTGGATAGTGGAAAAAGTA+CGG | + | chr8_4:50349730-50349749 | Msa1331020:CDS | 35.0% |
| !! | TTCGCACATCAAGTTGACAA+TGG | + | chr8_4:50349557-50349576 | Msa1331020:CDS | 40.0% |
| ! | GTCGGTACAAGTTTTTGTGA+TGG | + | chr8_4:50349596-50349615 | Msa1331020:CDS | 40.0% |
| ! | CGGTACAAGTTTTTGTGATG+GGG | + | chr8_4:50349598-50349617 | Msa1331020:CDS | 40.0% |
| CCAAGTTAACCTCTTTCCTA+CGG | - | chr8_4:50349715-50349734 | None:intergenic | 40.0% | |
| ! | GGTGTTGTACTCCACAATGT+CGG | + | chr8_4:50349578-50349597 | Msa1331020:CDS | 45.0% |
| CCGTAGGAAAGAGGTTAACT+TGG | + | chr8_4:50349712-50349731 | Msa1331020:CDS | 45.0% | |
| GCAATACAACAGCAGACCGT+AGG | + | chr8_4:50349696-50349715 | Msa1331020:intron | 50.0% | |
| AACAGCAGACCGTAGGAAAG+AGG | + | chr8_4:50349703-50349722 | Msa1331020:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 50349527 | 50349796 | 50349527 | ID=Msa1331020;Name=Msa1331020 |
| chr8_4 | mRNA | 50349527 | 50349796 | 50349527 | ID=Msa1331020-mRNA-1;Parent=Msa1331020;Name=Msa1331020-mRNA-1;_AED=0.63;_eAED=0.63;_QI=0|0|0|0.5|0|0.5|2|0|86 |
| chr8_4 | exon | 50349527 | 50349687 | 50349527 | ID=Msa1331020-mRNA-1:exon:14707;Parent=Msa1331020-mRNA-1 |
| chr8_4 | exon | 50349697 | 50349796 | 50349697 | ID=Msa1331020-mRNA-1:exon:14708;Parent=Msa1331020-mRNA-1 |
| chr8_4 | CDS | 50349527 | 50349687 | 50349527 | ID=Msa1331020-mRNA-1:cds;Parent=Msa1331020-mRNA-1 |
| chr8_4 | CDS | 50349697 | 50349796 | 50349697 | ID=Msa1331020-mRNA-1:cds;Parent=Msa1331020-mRNA-1 |
| Gene Sequence |
| Protein sequence |
