Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1204550 | RHN63009.1 | 96.296 | 54 | 2 | 0 | 1 | 54 | 113 | 166 | 3.19e-28 | 110 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0743950 | Msa1204550 | 0.807235 | 5.581474e-50 | -8.615850e-47 |
| Msa0309770 | Msa1204550 | 0.807935 | 3.963273e-50 | -8.615850e-47 |
| Msa1204550 | Msa1331020 | 0.928849 | 1.612423e-92 | -8.615850e-47 |
| Msa1204550 | Msa1373030 | 0.812348 | 4.435218e-51 | -8.615850e-47 |
| Msa1204550 | Msa1410100 | 0.803656 | 3.141943e-49 | -8.615850e-47 |
| Msa0864250 | Msa1204550 | 0.800138 | 1.658532e-48 | -8.615850e-47 |
| Msa0897330 | Msa1204550 | 0.800046 | 1.731777e-48 | -8.615850e-47 |
| Msa0938220 | Msa1204550 | 0.800401 | 1.466592e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1204550 | MtrunA17_Chr4g0053671 | 96.296 | 54 | 2 | 0 | 1 | 54 | 113 | 166 | 2.94e-32 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACAATAGAATATAAACTAA+TGG | 0.278862 | 8_1:-60308728 | None:intergenic |
| ACAATAGAATATAAACTAAT+GGG | 0.357341 | 8_1:-60308727 | None:intergenic |
| AAAGAGGTTAACTTGGATAG+TGG | 0.467598 | 8_1:+60308779 | Msa1204550:CDS |
| TGTTATACTCTATTTCTAAG+AGG | 0.486122 | 8_1:-60308703 | None:intergenic |
| CCGTAGGAAAGAGGTTAACT+TGG | 0.506970 | 8_1:+60308772 | Msa1204550:CDS |
| CCAAGTTAACCTCTTTCCTA+CGG | 0.567694 | 8_1:-60308772 | None:intergenic |
| AACAGCAGACCGTAGGAAAG+AGG | 0.579266 | 8_1:+60308763 | Msa1204550:CDS |
| GCAATACAACAGCAGACCGT+AGG | 0.615669 | 8_1:+60308756 | Msa1204550:intron |
| CATGGTTACAAACAGTGAAG+TGG | 0.661662 | 8_1:+60308586 | None:intergenic |
| TTCGCACATCAAGTTGACAA+TGG | 0.665212 | 8_1:+60308617 | Msa1204550:CDS |
| GGTGTTGTACTCCACAATGT+CGG | 0.682313 | 8_1:+60308638 | Msa1204550:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAATAGAATATAAACTAAT+GGG | - | chr8_1:60308730-60308749 | None:intergenic | 15.0% |
| !! | TACAATAGAATATAAACTAA+TGG | - | chr8_1:60308731-60308750 | None:intergenic | 15.0% |
| !!! | TGTTATACTCTATTTCTAAG+AGG | - | chr8_1:60308706-60308725 | None:intergenic | 25.0% |
| ! | AAAAACTTGTACCGACATTG+TGG | - | chr8_1:60308652-60308671 | None:intergenic | 35.0% |
| ! | TCGGTACAAGTTTTTGTGAT+GGG | + | chr8_1:60308657-60308676 | Msa1204550:CDS | 35.0% |
| ! | AAAGAGGTTAACTTGGATAG+TGG | + | chr8_1:60308779-60308798 | Msa1204550:CDS | 35.0% |
| !! | CTTGGATAGTGGAAAAAGTA+CGG | + | chr8_1:60308790-60308809 | Msa1204550:CDS | 35.0% |
| !! | TTCGCACATCAAGTTGACAA+TGG | + | chr8_1:60308617-60308636 | Msa1204550:CDS | 40.0% |
| ! | GTCGGTACAAGTTTTTGTGA+TGG | + | chr8_1:60308656-60308675 | Msa1204550:CDS | 40.0% |
| ! | CGGTACAAGTTTTTGTGATG+GGG | + | chr8_1:60308658-60308677 | Msa1204550:CDS | 40.0% |
| CCAAGTTAACCTCTTTCCTA+CGG | - | chr8_1:60308775-60308794 | None:intergenic | 40.0% | |
| ! | GGTGTTGTACTCCACAATGT+CGG | + | chr8_1:60308638-60308657 | Msa1204550:CDS | 45.0% |
| CCGTAGGAAAGAGGTTAACT+TGG | + | chr8_1:60308772-60308791 | Msa1204550:CDS | 45.0% | |
| GCAATACAACAGCAGACCGT+AGG | + | chr8_1:60308756-60308775 | Msa1204550:intron | 50.0% | |
| AACAGCAGACCGTAGGAAAG+AGG | + | chr8_1:60308763-60308782 | Msa1204550:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 60308587 | 60308856 | 60308587 | ID=Msa1204550;Name=Msa1204550 |
| chr8_1 | mRNA | 60308587 | 60308856 | 60308587 | ID=Msa1204550-mRNA-1;Parent=Msa1204550;Name=Msa1204550-mRNA-1;_AED=0.62;_eAED=0.62;_QI=0|0|0|0.5|0|0|2|0|86 |
| chr8_1 | exon | 60308587 | 60308747 | 60308587 | ID=Msa1204550-mRNA-1:exon:19588;Parent=Msa1204550-mRNA-1 |
| chr8_1 | exon | 60308757 | 60308856 | 60308757 | ID=Msa1204550-mRNA-1:exon:19589;Parent=Msa1204550-mRNA-1 |
| chr8_1 | CDS | 60308587 | 60308747 | 60308587 | ID=Msa1204550-mRNA-1:cds;Parent=Msa1204550-mRNA-1 |
| chr8_1 | CDS | 60308757 | 60308856 | 60308757 | ID=Msa1204550-mRNA-1:cds;Parent=Msa1204550-mRNA-1 |
| Gene Sequence |
| Protein sequence |
