AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1332030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1332030 MtrunA17_Chr4g0064101 89.450 218 4 2 2 201 918 1134 2.43e-129 390
Msa1332030 MtrunA17_Chr4g0003011 60.987 223 63 3 2 201 916 1137 4.16e-83 266
Msa1332030 MtrunA17_Chr2g0333311 63.682 201 54 2 2 184 915 1114 1.27e-80 259
Msa1332030 MtrunA17_Chr1g0192431 54.348 230 69 7 2 201 905 1128 6.08e-69 227
Msa1332030 MtrunA17_Chr7g0271991 55.122 205 69 4 2 185 907 1109 3.05e-67 222
Msa1332030 MtrunA17_Chr4g0067791 50.459 218 90 3 2 201 905 1122 3.13e-65 217
Msa1332030 MtrunA17_Chr2g0295351 47.980 198 82 4 5 182 786 982 1.87e-52 180
Msa1332030 MtrunA17_Chr2g0295251 44.845 194 86 4 2 175 605 797 2.84e-48 167
Msa1332030 MtrunA17_Chr1g0149541 39.583 192 89 4 6 175 110 296 6.42e-42 143
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1332030 AT1G80490.3 60.360 222 66 3 2 201 899 1120 5.29e-84 269
Msa1332030 AT1G80490.2 60.360 222 66 3 2 201 899 1120 5.29e-84 269
Msa1332030 AT1G80490.1 60.360 222 66 3 2 201 898 1119 5.93e-84 269
Msa1332030 AT3G15880.3 59.447 217 69 2 2 200 910 1125 8.62e-83 266
Msa1332030 AT3G15880.1 59.447 217 69 2 2 200 920 1135 1.16e-82 266
Msa1332030 AT3G15880.4 59.447 217 69 2 2 200 920 1135 1.16e-82 266
Msa1332030 AT3G15880.2 59.722 216 68 2 2 199 920 1134 1.30e-82 266
Msa1332030 AT1G15750.3 58.559 222 70 3 2 201 910 1131 1.53e-81 263
Msa1332030 AT1G15750.1 58.559 222 70 3 2 201 910 1131 1.53e-81 263
Msa1332030 AT1G15750.2 58.559 222 70 3 2 201 910 1131 1.53e-81 263
Msa1332030 AT1G15750.4 58.559 222 70 3 2 201 910 1131 1.53e-81 263
Msa1332030 AT5G27030.1 53.881 219 77 5 2 201 895 1108 4.96e-69 228
Msa1332030 AT5G27030.2 53.881 219 77 5 2 201 921 1134 6.82e-69 227
Msa1332030 AT3G16830.1 55.897 195 64 4 2 176 906 1098 1.41e-62 209
Msa1332030 AT2G25420.1 40.000 205 99 4 2 183 525 728 1.59e-38 140
Msa1332030 AT2G25420.4 40.000 205 99 4 2 183 532 735 1.68e-38 140
Msa1332030 AT2G25420.3 35.583 163 84 2 2 143 525 687 4.57e-23 96.7
Msa1332030 AT2G25420.2 35.583 163 84 2 2 143 532 694 4.58e-23 96.7

Find 91 sgRNAs with CRISPR-Local

Find 250 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTTAGGCATTCCAATTTC+GGG 0.163326 8_4:+51836406 None:intergenic
TGCTTTAGGCATTCCAATTT+CGG 0.177034 8_4:+51836405 None:intergenic
TCGAGTATGTTTACTCATTT+AGG 0.207724 8_4:-51835588 Msa1332030:three_prime_UTR
AAAATAAGACCCACCTAAAA+TGG 0.222726 8_4:+51835486 None:intergenic
CAATTTCGGGGCCTCATATA+TGG 0.277943 8_4:+51836419 None:intergenic
CATAATGCACTGTCACCTTT+GGG 0.281690 8_4:+51835789 None:intergenic
CATATATGAGGCCCCGAAAT+TGG 0.315351 8_4:-51836418 Msa1332030:CDS
CTAAATATTCTTGTATCATC+TGG 0.334907 8_4:-51836877 Msa1332030:CDS
GCATAATGCACTGTCACCTT+TGG 0.343316 8_4:+51835788 None:intergenic
GAATCCAATCAATTTGCATT+AGG 0.357642 8_4:-51835937 Msa1332030:CDS
GGTGCTGGAGCTCGCCCATT+TGG 0.361614 8_4:+51836513 None:intergenic
TAATGCAAATTGATTGGATT+CGG 0.395116 8_4:+51835939 None:intergenic
ACTTGTGTTTATAAGAAGAT+AGG 0.398147 8_4:-51835675 Msa1332030:three_prime_UTR
TGAAATGCATAGCGTAAATT+CGG 0.413459 8_4:-51835557 Msa1332030:three_prime_UTR
AAGTCCTAATGCAAATTGAT+TGG 0.415003 8_4:+51835933 None:intergenic
GTCTAATCAGTGGGTGCCTC+GGG 0.416068 8_4:-51836235 Msa1332030:intron
CAAACCAACACACCTGCTTT+AGG 0.421274 8_4:+51836391 None:intergenic
CCGCCCCTGGTGCCTCCATT+CGG 0.428429 8_4:-51835719 Msa1332030:three_prime_UTR
GTTGGTCCTAGCACTCCTTC+AGG 0.434107 8_4:-51835832 Msa1332030:CDS
ATTATGCAGTGCTGTCTCTA+AGG 0.437257 8_4:-51835772 Msa1332030:three_prime_UTR
CGAGTATCTGCAAGAGGTGC+TGG 0.438201 8_4:+51836498 None:intergenic
CGAATGGAGGCACCAGGGGC+GGG 0.442889 8_4:+51835720 None:intergenic
GGAAGCACTAACAAGGTGAT+TGG 0.447092 8_4:+51835638 None:intergenic
AAATTCCTACAGATGCCAAA+TGG 0.451156 8_4:-51836528 Msa1332030:intron
GTGCCTCCATTCGGTTTGCA+AGG 0.453373 8_4:-51835710 Msa1332030:three_prime_UTR
TAACAAAACGCACCTTGGTT+GGG 0.456481 8_4:+51836069 None:intergenic
CCATGCAGAAGAAAATGATT+AGG 0.457989 8_4:+51835617 None:intergenic
CCGAATGGAGGCACCAGGGG+CGG 0.461135 8_4:+51835719 None:intergenic
ATAACAAAACGCACCTTGGT+TGG 0.461763 8_4:+51836068 None:intergenic
AAGGTCAAAACCAAGCTAAA+AGG 0.464928 8_4:-51836940 Msa1332030:CDS
ATGCATAGCGTAAATTCGGC+TGG 0.471411 8_4:-51835553 Msa1332030:three_prime_UTR
ATTGCTTGTGGTCCCGCCCC+TGG 0.476945 8_4:-51835732 Msa1332030:three_prime_UTR
GCTAGGACCAACACCATTCT+CGG 0.481044 8_4:+51835843 None:intergenic
ATCATCTGGAGCTGACTCTC+AGG 0.487968 8_4:-51836863 Msa1332030:intron
GCTGGGGCGTTGCAGAGTGG+AGG 0.488555 8_4:-51835535 Msa1332030:three_prime_UTR
TGTCTAATCAGTGGGTGCCT+CGG 0.491371 8_4:-51836236 Msa1332030:intron
GAATATGTAGCATGTGTGAT+TGG 0.499345 8_4:+51836201 None:intergenic
TCTCCACCTCCAACCGAGAA+TGG 0.505262 8_4:-51835856 Msa1332030:CDS
GAACAGCAAGTAAATGTGTC+TGG 0.505559 8_4:+51836457 None:intergenic
GCTGTCTCTAAGGACCTTTG+AGG 0.506192 8_4:-51835762 Msa1332030:three_prime_UTR
AATTCCTACAGATGCCAAAT+GGG 0.506981 8_4:-51836527 Msa1332030:intron
GCAAACCGAATGGAGGCACC+AGG 0.508441 8_4:+51835714 None:intergenic
AGCTTTGAAGATGGAAGTAT+TGG 0.509193 8_4:-51836153 Msa1332030:CDS
TGCCTCCATTCGGTTTGCAA+GGG 0.509350 8_4:-51835709 Msa1332030:three_prime_UTR
TGCAAATTGATTGGATTCGG+AGG 0.509795 8_4:+51835942 None:intergenic
TGAGGTGTATTTATTGCTTG+TGG 0.515384 8_4:-51835744 Msa1332030:three_prime_UTR
GGTCATCAGAAGAGAATCAC+TGG 0.518820 8_4:-51836919 Msa1332030:CDS
TCACAAGAGGATATACTCTC+AGG 0.522387 8_4:+51835977 None:intergenic
TGCATAGCGTAAATTCGGCT+GGG 0.532971 8_4:-51835552 Msa1332030:three_prime_UTR
CTGGGGCGTTGCAGAGTGGA+GGG 0.533130 8_4:-51835534 Msa1332030:three_prime_UTR
AACTCAAATAGCCATATATG+AGG 0.533924 8_4:-51836430 Msa1332030:CDS
TTACTTCTACATTCGATGCA+AGG 0.538499 8_4:-51835377 Msa1332030:three_prime_UTR
TTGGGGTTGCTCTGAAACAG+AGG 0.546190 8_4:+51835807 None:intergenic
GCAAATTGATTGGATTCGGA+GGG 0.551991 8_4:+51835943 None:intergenic
ACACCATTCTCGGTTGGAGG+TGG 0.552915 8_4:+51835853 None:intergenic
ATTAAATATCTGAGTAACAC+TGG 0.554834 8_4:+51835407 None:intergenic
CCAACACCATTCTCGGTTGG+AGG 0.558356 8_4:+51835850 None:intergenic
ATATATGTAAGCTTTGAAGA+TGG 0.562266 8_4:-51836162 Msa1332030:CDS
GCATTAGGACTTACAGATGG+TGG 0.565249 8_4:-51835922 Msa1332030:CDS
CACCTTCCTTCTGACTCTAG+TGG 0.566807 8_4:+51835883 None:intergenic
GCCTCCATTCGGTTTGCAAG+GGG 0.567752 8_4:-51835708 Msa1332030:three_prime_UTR
GAAATTGGAATGCCTAAAGC+AGG 0.577051 8_4:-51836403 Msa1332030:intron
CTTTAGGCATTCCAATTTCG+GGG 0.582062 8_4:+51836407 None:intergenic
ACTGATAACAAAACGCACCT+TGG 0.583084 8_4:+51836064 None:intergenic
CACTAGAGTCAGAAGGAAGG+TGG 0.584662 8_4:-51835882 Msa1332030:CDS
ATAATGCACTGTCACCTTTG+GGG 0.593411 8_4:+51835790 None:intergenic
GGACCAACACCATTCTCGGT+TGG 0.593764 8_4:+51835847 None:intergenic
GCTTCCCCTTGCAAACCGAA+TGG 0.594040 8_4:+51835704 None:intergenic
ACATCTTAATCTGAGTGTTG+AGG 0.596731 8_4:+51836119 None:intergenic
TGGGTGCCTCGGGAAGCTAG+TGG 0.598278 8_4:-51836225 Msa1332030:CDS
AAACCGAATGGAGGCACCAG+GGG 0.598609 8_4:+51835716 None:intergenic
TCGGCTGGGGCGTTGCAGAG+TGG 0.599512 8_4:-51835538 Msa1332030:three_prime_UTR
CCTCCAACCGAGAATGGTGT+TGG 0.600489 8_4:-51835850 Msa1332030:CDS
GAGGCACCTGAAGGAGTGCT+AGG 0.602557 8_4:+51835826 None:intergenic
ACTAGAGTCAGAAGGAAGGT+GGG 0.605468 8_4:-51835881 Msa1332030:CDS
ATGATTAGGAAGCACTAACA+AGG 0.610488 8_4:+51835631 None:intergenic
AAGCAATAAATACACCTCAA+AGG 0.613934 8_4:+51835748 None:intergenic
GCATAGCGTAAATTCGGCTG+GGG 0.617506 8_4:-51835551 Msa1332030:three_prime_UTR
CGGTTTGCAAGGGGAAGCTG+CGG 0.619936 8_4:-51835699 Msa1332030:three_prime_UTR
TTTGCATTAGGACTTACAGA+TGG 0.625618 8_4:-51835925 Msa1332030:CDS
CTTGAACCACTAGAGTCAGA+AGG 0.630820 8_4:-51835889 Msa1332030:CDS
TCTGAAACAGAGGCACCTGA+AGG 0.631626 8_4:+51835817 None:intergenic
CTAGAGTCAGAAGGAAGGTG+GGG 0.631856 8_4:-51835880 Msa1332030:CDS
GGATGTGCAGCGATCACAAG+AGG 0.633657 8_4:+51835964 None:intergenic
TGAACACGAGTATCTGCAAG+AGG 0.642790 8_4:+51836492 None:intergenic
TCCCCTTGCAAACCGAATGG+AGG 0.643500 8_4:+51835707 None:intergenic
CAAACCGAATGGAGGCACCA+GGG 0.646185 8_4:+51835715 None:intergenic
CTGTTTCAGAGCAACCCCAA+AGG 0.660357 8_4:-51835804 Msa1332030:CDS
AACCACTAGAGTCAGAAGGA+AGG 0.676019 8_4:-51835885 Msa1332030:CDS
GCTTATCTCAATCCCAACCA+AGG 0.686653 8_4:-51836081 Msa1332030:CDS
ATTGGACCACTAGCTTCCCG+AGG 0.718296 8_4:+51836219 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTATGCAGTTTTTATTT+TGG - chr8_4:51835957-51835976 Msa1332030:CDS 15.0%
!!! TTGTGTAGATTTTAAAAAAT+AGG - chr8_4:51836852-51836871 Msa1332030:intron 15.0%
!!! TGTGTAGATTTTAAAAAATA+GGG - chr8_4:51836853-51836872 Msa1332030:intron 15.0%
!!! AATTTATGCAGTTTTTATTT+TGG - chr8_4:51835957-51835976 Msa1332030:CDS 15.0%
!!! TTGTGTAGATTTTAAAAAAT+AGG - chr8_4:51836852-51836871 Msa1332030:intron 15.0%
!!! TGTGTAGATTTTAAAAAATA+GGG - chr8_4:51836853-51836872 Msa1332030:intron 15.0%
!!! TAAAGATTACTTTTAGCTTT+TGG + chr8_4:51835522-51835541 None:intergenic 20.0%
!!! TAAAGATTACTTTTAGCTTT+TGG + chr8_4:51835522-51835541 None:intergenic 20.0%
! CTAAATATTCTTGTATCATC+TGG - chr8_4:51835436-51835455 Msa1332030:three_prime_UTR 25.0%
! GTTAATAACGATGTATTTAG+TGG - chr8_4:51835683-51835702 Msa1332030:three_prime_UTR 25.0%
! TTGAAATTCAGTATTCTTCA+TGG + chr8_4:51836057-51836076 None:intergenic 25.0%
! AATTTCAATGTCTAATCAGT+GGG - chr8_4:51836069-51836088 Msa1332030:intron 25.0%
!!! ATATATGTAAGCTTTGAAGA+TGG - chr8_4:51836151-51836170 Msa1332030:CDS 25.0%
! TAATGCAAATTGATTGGATT+CGG + chr8_4:51836377-51836396 None:intergenic 25.0%
! ACTTGTGTTTATAAGAAGAT+AGG - chr8_4:51836638-51836657 Msa1332030:intron 25.0%
!! TTTCTAGGTTTTTCCATTTT+AGG - chr8_4:51836814-51836833 Msa1332030:intron 25.0%
! ATTAAATATCTGAGTAACAC+TGG + chr8_4:51836909-51836928 None:intergenic 25.0%
! CTAAATATTCTTGTATCATC+TGG - chr8_4:51835436-51835455 Msa1332030:three_prime_UTR 25.0%
! GTTAATAACGATGTATTTAG+TGG - chr8_4:51835683-51835702 Msa1332030:three_prime_UTR 25.0%
! TTGAAATTCAGTATTCTTCA+TGG + chr8_4:51836057-51836076 None:intergenic 25.0%
! AATTTCAATGTCTAATCAGT+GGG - chr8_4:51836069-51836088 Msa1332030:intron 25.0%
!!! ATATATGTAAGCTTTGAAGA+TGG - chr8_4:51836151-51836170 Msa1332030:CDS 25.0%
! TAATGCAAATTGATTGGATT+CGG + chr8_4:51836377-51836396 None:intergenic 25.0%
! ACTTGTGTTTATAAGAAGAT+AGG - chr8_4:51836638-51836657 Msa1332030:intron 25.0%
!! TTTCTAGGTTTTTCCATTTT+AGG - chr8_4:51836814-51836833 Msa1332030:intron 25.0%
! ATTAAATATCTGAGTAACAC+TGG + chr8_4:51836909-51836928 None:intergenic 25.0%
CACTCTATTTATAGAGCAAA+AGG + chr8_4:51835496-51835515 None:intergenic 30.0%
GACAAACGATGAAAAATCTA+AGG + chr8_4:51835592-51835611 None:intergenic 30.0%
TTAATGATCATACTTTCGCA+AGG + chr8_4:51835663-51835682 None:intergenic 30.0%
ATGTCAAATAGTTGTGTGTA+TGG - chr8_4:51835732-51835751 Msa1332030:three_prime_UTR 30.0%
AACTCAAATAGCCATATATG+AGG - chr8_4:51835883-51835902 Msa1332030:CDS 30.0%
!!! ATGCAGTTTTTATTTTGGTC+TGG - chr8_4:51835962-51835981 Msa1332030:CDS 30.0%
GAGCGCATTGTTTATTTAAT+TGG - chr8_4:51835987-51836006 Msa1332030:intron 30.0%
GAATTTCAATGTCTAATCAG+TGG - chr8_4:51836068-51836087 Msa1332030:intron 30.0%
GCTGTCAAAAAGAAGTTATT+TGG + chr8_4:51836317-51836336 None:intergenic 30.0%
GAATCCAATCAATTTGCATT+AGG - chr8_4:51836376-51836395 Msa1332030:intron 30.0%
AAGTCCTAATGCAAATTGAT+TGG + chr8_4:51836383-51836402 None:intergenic 30.0%
AAGCAATAAATACACCTCAA+AGG + chr8_4:51836568-51836587 None:intergenic 30.0%
! CTAATCATTTTCTTCTGCAT+GGG - chr8_4:51836697-51836716 Msa1332030:intron 30.0%
! TAATCATTTTCTTCTGCATG+GGG - chr8_4:51836698-51836717 Msa1332030:intron 30.0%
TCGAGTATGTTTACTCATTT+AGG - chr8_4:51836725-51836744 Msa1332030:intron 30.0%
TGAAATGCATAGCGTAAATT+CGG - chr8_4:51836756-51836775 Msa1332030:intron 30.0%
!!! GGGTCATTGTTATTTTTTCT+AGG - chr8_4:51836799-51836818 Msa1332030:intron 30.0%
!! TAGGTTTTTCCATTTTAGGT+GGG - chr8_4:51836818-51836837 Msa1332030:intron 30.0%
AAAATAAGACCCACCTAAAA+TGG + chr8_4:51836830-51836849 None:intergenic 30.0%
CACTCTATTTATAGAGCAAA+AGG + chr8_4:51835496-51835515 None:intergenic 30.0%
GACAAACGATGAAAAATCTA+AGG + chr8_4:51835592-51835611 None:intergenic 30.0%
TTAATGATCATACTTTCGCA+AGG + chr8_4:51835663-51835682 None:intergenic 30.0%
ATGTCAAATAGTTGTGTGTA+TGG - chr8_4:51835732-51835751 Msa1332030:three_prime_UTR 30.0%
AACTCAAATAGCCATATATG+AGG - chr8_4:51835883-51835902 Msa1332030:CDS 30.0%
!!! ATGCAGTTTTTATTTTGGTC+TGG - chr8_4:51835962-51835981 Msa1332030:CDS 30.0%
GAGCGCATTGTTTATTTAAT+TGG - chr8_4:51835987-51836006 Msa1332030:intron 30.0%
GAATTTCAATGTCTAATCAG+TGG - chr8_4:51836068-51836087 Msa1332030:intron 30.0%
GCTGTCAAAAAGAAGTTATT+TGG + chr8_4:51836317-51836336 None:intergenic 30.0%
GAATCCAATCAATTTGCATT+AGG - chr8_4:51836376-51836395 Msa1332030:intron 30.0%
AAGTCCTAATGCAAATTGAT+TGG + chr8_4:51836383-51836402 None:intergenic 30.0%
AAGCAATAAATACACCTCAA+AGG + chr8_4:51836568-51836587 None:intergenic 30.0%
! CTAATCATTTTCTTCTGCAT+GGG - chr8_4:51836697-51836716 Msa1332030:intron 30.0%
! TAATCATTTTCTTCTGCATG+GGG - chr8_4:51836698-51836717 Msa1332030:intron 30.0%
TCGAGTATGTTTACTCATTT+AGG - chr8_4:51836725-51836744 Msa1332030:intron 30.0%
TGAAATGCATAGCGTAAATT+CGG - chr8_4:51836756-51836775 Msa1332030:intron 30.0%
!!! GGGTCATTGTTATTTTTTCT+AGG - chr8_4:51836799-51836818 Msa1332030:intron 30.0%
!! TAGGTTTTTCCATTTTAGGT+GGG - chr8_4:51836818-51836837 Msa1332030:intron 30.0%
AAAATAAGACCCACCTAAAA+TGG + chr8_4:51836830-51836849 None:intergenic 30.0%
AAGGTCAAAACCAAGCTAAA+AGG - chr8_4:51835373-51835392 Msa1332030:three_prime_UTR 35.0%
TTGTTTGCCTACATATTCCA+AGG - chr8_4:51835557-51835576 Msa1332030:three_prime_UTR 35.0%
AACACAACCTTGGAATATGT+AGG + chr8_4:51835567-51835586 None:intergenic 35.0%
! ATCTAAGGTAAACACAACCT+TGG + chr8_4:51835577-51835596 None:intergenic 35.0%
ACATAATGCACAGAGCATAA+CGG + chr8_4:51835639-51835658 None:intergenic 35.0%
AAATTCCTACAGATGCCAAA+TGG - chr8_4:51835785-51835804 Msa1332030:exon 35.0%
AATTCCTACAGATGCCAAAT+GGG - chr8_4:51835786-51835805 Msa1332030:exon 35.0%
! TGCTTTAGGCATTCCAATTT+CGG + chr8_4:51835911-51835930 None:intergenic 35.0%
GAATATGTAGCATGTGTGAT+TGG + chr8_4:51836115-51836134 None:intergenic 35.0%
!! AGCTTTGAAGATGGAAGTAT+TGG - chr8_4:51836160-51836179 Msa1332030:CDS 35.0%
! ACATCTTAATCTGAGTGTTG+AGG + chr8_4:51836197-51836216 None:intergenic 35.0%
TTTGCATTAGGACTTACAGA+TGG - chr8_4:51836388-51836407 Msa1332030:intron 35.0%
TGAGGTGTATTTATTGCTTG+TGG - chr8_4:51836569-51836588 Msa1332030:intron 35.0%
!! ATGATTAGGAAGCACTAACA+AGG + chr8_4:51836685-51836704 None:intergenic 35.0%
CCATGCAGAAGAAAATGATT+AGG + chr8_4:51836699-51836718 None:intergenic 35.0%
! CCTAATCATTTTCTTCTGCA+TGG - chr8_4:51836696-51836715 Msa1332030:intron 35.0%
! CTAGGTTTTTCCATTTTAGG+TGG - chr8_4:51836817-51836836 Msa1332030:intron 35.0%
TTACTTCTACATTCGATGCA+AGG - chr8_4:51836936-51836955 Msa1332030:CDS 35.0%
AAGGTCAAAACCAAGCTAAA+AGG - chr8_4:51835373-51835392 Msa1332030:three_prime_UTR 35.0%
TTGTTTGCCTACATATTCCA+AGG - chr8_4:51835557-51835576 Msa1332030:three_prime_UTR 35.0%
AACACAACCTTGGAATATGT+AGG + chr8_4:51835567-51835586 None:intergenic 35.0%
! ATCTAAGGTAAACACAACCT+TGG + chr8_4:51835577-51835596 None:intergenic 35.0%
ACATAATGCACAGAGCATAA+CGG + chr8_4:51835639-51835658 None:intergenic 35.0%
AAATTCCTACAGATGCCAAA+TGG - chr8_4:51835785-51835804 Msa1332030:exon 35.0%
AATTCCTACAGATGCCAAAT+GGG - chr8_4:51835786-51835805 Msa1332030:exon 35.0%
! TGCTTTAGGCATTCCAATTT+CGG + chr8_4:51835911-51835930 None:intergenic 35.0%
GAATATGTAGCATGTGTGAT+TGG + chr8_4:51836115-51836134 None:intergenic 35.0%
!! AGCTTTGAAGATGGAAGTAT+TGG - chr8_4:51836160-51836179 Msa1332030:CDS 35.0%
! ACATCTTAATCTGAGTGTTG+AGG + chr8_4:51836197-51836216 None:intergenic 35.0%
TTTGCATTAGGACTTACAGA+TGG - chr8_4:51836388-51836407 Msa1332030:intron 35.0%
TGAGGTGTATTTATTGCTTG+TGG - chr8_4:51836569-51836588 Msa1332030:intron 35.0%
!! ATGATTAGGAAGCACTAACA+AGG + chr8_4:51836685-51836704 None:intergenic 35.0%
CCATGCAGAAGAAAATGATT+AGG + chr8_4:51836699-51836718 None:intergenic 35.0%
! CCTAATCATTTTCTTCTGCA+TGG - chr8_4:51836696-51836715 Msa1332030:intron 35.0%
! CTAGGTTTTTCCATTTTAGG+TGG - chr8_4:51836817-51836836 Msa1332030:intron 35.0%
TTACTTCTACATTCGATGCA+AGG - chr8_4:51836936-51836955 Msa1332030:CDS 35.0%
!! CTTCTGATGACCTTTTAGCT+TGG + chr8_4:51835386-51835405 None:intergenic 40.0%
ATAGTTGTGTGTATGGAGCA+CGG - chr8_4:51835739-51835758 Msa1332030:three_prime_UTR 40.0%
GAACAGCAAGTAAATGTGTC+TGG + chr8_4:51835859-51835878 None:intergenic 40.0%
CTTTAGGCATTCCAATTTCG+GGG + chr8_4:51835909-51835928 None:intergenic 40.0%
! GCTTTAGGCATTCCAATTTC+GGG + chr8_4:51835910-51835929 None:intergenic 40.0%
GAAATTGGAATGCCTAAAGC+AGG - chr8_4:51835910-51835929 Msa1332030:CDS 40.0%
TAACAAAACGCACCTTGGTT+GGG + chr8_4:51836247-51836266 None:intergenic 40.0%
ATAACAAAACGCACCTTGGT+TGG + chr8_4:51836248-51836267 None:intergenic 40.0%
ACTGATAACAAAACGCACCT+TGG + chr8_4:51836252-51836271 None:intergenic 40.0%
!! AAGGTGCGTTTTGTTATCAG+TGG - chr8_4:51836251-51836270 Msa1332030:intron 40.0%
TCACAAGAGGATATACTCTC+AGG + chr8_4:51836339-51836358 None:intergenic 40.0%
GCAAATTGATTGGATTCGGA+GGG + chr8_4:51836373-51836392 None:intergenic 40.0%
TGCAAATTGATTGGATTCGG+AGG + chr8_4:51836374-51836393 None:intergenic 40.0%
ATAATGCACTGTCACCTTTG+GGG + chr8_4:51836526-51836545 None:intergenic 40.0%
CATAATGCACTGTCACCTTT+GGG + chr8_4:51836527-51836546 None:intergenic 40.0%
ATTATGCAGTGCTGTCTCTA+AGG - chr8_4:51836541-51836560 Msa1332030:intron 40.0%
!! CTTCTGATGACCTTTTAGCT+TGG + chr8_4:51835386-51835405 None:intergenic 40.0%
ATAGTTGTGTGTATGGAGCA+CGG - chr8_4:51835739-51835758 Msa1332030:three_prime_UTR 40.0%
GAACAGCAAGTAAATGTGTC+TGG + chr8_4:51835859-51835878 None:intergenic 40.0%
CTTTAGGCATTCCAATTTCG+GGG + chr8_4:51835909-51835928 None:intergenic 40.0%
! GCTTTAGGCATTCCAATTTC+GGG + chr8_4:51835910-51835929 None:intergenic 40.0%
GAAATTGGAATGCCTAAAGC+AGG - chr8_4:51835910-51835929 Msa1332030:CDS 40.0%
TAACAAAACGCACCTTGGTT+GGG + chr8_4:51836247-51836266 None:intergenic 40.0%
ATAACAAAACGCACCTTGGT+TGG + chr8_4:51836248-51836267 None:intergenic 40.0%
ACTGATAACAAAACGCACCT+TGG + chr8_4:51836252-51836271 None:intergenic 40.0%
!! AAGGTGCGTTTTGTTATCAG+TGG - chr8_4:51836251-51836270 Msa1332030:intron 40.0%
TCACAAGAGGATATACTCTC+AGG + chr8_4:51836339-51836358 None:intergenic 40.0%
GCAAATTGATTGGATTCGGA+GGG + chr8_4:51836373-51836392 None:intergenic 40.0%
TGCAAATTGATTGGATTCGG+AGG + chr8_4:51836374-51836393 None:intergenic 40.0%
ATAATGCACTGTCACCTTTG+GGG + chr8_4:51836526-51836545 None:intergenic 40.0%
CATAATGCACTGTCACCTTT+GGG + chr8_4:51836527-51836546 None:intergenic 40.0%
ATTATGCAGTGCTGTCTCTA+AGG - chr8_4:51836541-51836560 Msa1332030:intron 40.0%
GGTCATCAGAAGAGAATCAC+TGG - chr8_4:51835394-51835413 Msa1332030:three_prime_UTR 45.0%
TGAACACGAGTATCTGCAAG+AGG + chr8_4:51835824-51835843 None:intergenic 45.0%
CAATTTCGGGGCCTCATATA+TGG + chr8_4:51835897-51835916 None:intergenic 45.0%
CATATATGAGGCCCCGAAAT+TGG - chr8_4:51835895-51835914 Msa1332030:CDS 45.0%
AATGCCTAAAGCAGGTGTGT+TGG - chr8_4:51835918-51835937 Msa1332030:CDS 45.0%
CAAACCAACACACCTGCTTT+AGG + chr8_4:51835925-51835944 None:intergenic 45.0%
GGTAGCTCATCATCTGATCT+TGG - chr8_4:51836008-51836027 Msa1332030:intron 45.0%
GCTTATCTCAATCCCAACCA+AGG - chr8_4:51836232-51836251 Msa1332030:intron 45.0%
GCATTAGGACTTACAGATGG+TGG - chr8_4:51836391-51836410 Msa1332030:intron 45.0%
CTTGAACCACTAGAGTCAGA+AGG - chr8_4:51836424-51836443 Msa1332030:CDS 45.0%
AACCACTAGAGTCAGAAGGA+AGG - chr8_4:51836428-51836447 Msa1332030:CDS 45.0%
ACTAGAGTCAGAAGGAAGGT+GGG - chr8_4:51836432-51836451 Msa1332030:CDS 45.0%
GCATAATGCACTGTCACCTT+TGG + chr8_4:51836528-51836547 None:intergenic 45.0%
!! GGAAGCACTAACAAGGTGAT+TGG + chr8_4:51836678-51836697 None:intergenic 45.0%
ATGCATAGCGTAAATTCGGC+TGG - chr8_4:51836760-51836779 Msa1332030:intron 45.0%
TGCATAGCGTAAATTCGGCT+GGG - chr8_4:51836761-51836780 Msa1332030:intron 45.0%
GGTCATCAGAAGAGAATCAC+TGG - chr8_4:51835394-51835413 Msa1332030:three_prime_UTR 45.0%
TGAACACGAGTATCTGCAAG+AGG + chr8_4:51835824-51835843 None:intergenic 45.0%
CAATTTCGGGGCCTCATATA+TGG + chr8_4:51835897-51835916 None:intergenic 45.0%
CATATATGAGGCCCCGAAAT+TGG - chr8_4:51835895-51835914 Msa1332030:CDS 45.0%
AATGCCTAAAGCAGGTGTGT+TGG - chr8_4:51835918-51835937 Msa1332030:CDS 45.0%
CAAACCAACACACCTGCTTT+AGG + chr8_4:51835925-51835944 None:intergenic 45.0%
GGTAGCTCATCATCTGATCT+TGG - chr8_4:51836008-51836027 Msa1332030:intron 45.0%
GCTTATCTCAATCCCAACCA+AGG - chr8_4:51836232-51836251 Msa1332030:intron 45.0%
GCATTAGGACTTACAGATGG+TGG - chr8_4:51836391-51836410 Msa1332030:intron 45.0%
CTTGAACCACTAGAGTCAGA+AGG - chr8_4:51836424-51836443 Msa1332030:CDS 45.0%
AACCACTAGAGTCAGAAGGA+AGG - chr8_4:51836428-51836447 Msa1332030:CDS 45.0%
ACTAGAGTCAGAAGGAAGGT+GGG - chr8_4:51836432-51836451 Msa1332030:CDS 45.0%
GCATAATGCACTGTCACCTT+TGG + chr8_4:51836528-51836547 None:intergenic 45.0%
!! GGAAGCACTAACAAGGTGAT+TGG + chr8_4:51836678-51836697 None:intergenic 45.0%
ATGCATAGCGTAAATTCGGC+TGG - chr8_4:51836760-51836779 Msa1332030:intron 45.0%
TGCATAGCGTAAATTCGGCT+GGG - chr8_4:51836761-51836780 Msa1332030:intron 45.0%
! ATCATCTGGAGCTGACTCTC+AGG - chr8_4:51835450-51835469 Msa1332030:three_prime_UTR 50.0%
TTGTGTGTATGGAGCACGGA+CGG - chr8_4:51835743-51835762 Msa1332030:three_prime_UTR 50.0%
TGTCTAATCAGTGGGTGCCT+CGG - chr8_4:51836077-51836096 Msa1332030:intron 50.0%
CACCTTCCTTCTGACTCTAG+TGG + chr8_4:51836433-51836452 None:intergenic 50.0%
CACTAGAGTCAGAAGGAAGG+TGG - chr8_4:51836431-51836450 Msa1332030:CDS 50.0%
CTAGAGTCAGAAGGAAGGTG+GGG - chr8_4:51836433-51836452 Msa1332030:CDS 50.0%
GCTAGGACCAACACCATTCT+CGG + chr8_4:51836473-51836492 None:intergenic 50.0%
!! TCTGAAACAGAGGCACCTGA+AGG + chr8_4:51836499-51836518 None:intergenic 50.0%
!! TTGGGGTTGCTCTGAAACAG+AGG + chr8_4:51836509-51836528 None:intergenic 50.0%
CTGTTTCAGAGCAACCCCAA+AGG - chr8_4:51836509-51836528 Msa1332030:CDS 50.0%
! GCTGTCTCTAAGGACCTTTG+AGG - chr8_4:51836551-51836570 Msa1332030:intron 50.0%
! TGCCTCCATTCGGTTTGCAA+GGG - chr8_4:51836604-51836623 Msa1332030:intron 50.0%
GCATAGCGTAAATTCGGCTG+GGG - chr8_4:51836762-51836781 Msa1332030:intron 50.0%
! ATCATCTGGAGCTGACTCTC+AGG - chr8_4:51835450-51835469 Msa1332030:three_prime_UTR 50.0%
TTGTGTGTATGGAGCACGGA+CGG - chr8_4:51835743-51835762 Msa1332030:three_prime_UTR 50.0%
TGTCTAATCAGTGGGTGCCT+CGG - chr8_4:51836077-51836096 Msa1332030:intron 50.0%
CACCTTCCTTCTGACTCTAG+TGG + chr8_4:51836433-51836452 None:intergenic 50.0%
CACTAGAGTCAGAAGGAAGG+TGG - chr8_4:51836431-51836450 Msa1332030:CDS 50.0%
CTAGAGTCAGAAGGAAGGTG+GGG - chr8_4:51836433-51836452 Msa1332030:CDS 50.0%
GCTAGGACCAACACCATTCT+CGG + chr8_4:51836473-51836492 None:intergenic 50.0%
!! TCTGAAACAGAGGCACCTGA+AGG + chr8_4:51836499-51836518 None:intergenic 50.0%
!! TTGGGGTTGCTCTGAAACAG+AGG + chr8_4:51836509-51836528 None:intergenic 50.0%
CTGTTTCAGAGCAACCCCAA+AGG - chr8_4:51836509-51836528 Msa1332030:CDS 50.0%
! GCTGTCTCTAAGGACCTTTG+AGG - chr8_4:51836551-51836570 Msa1332030:intron 50.0%
! TGCCTCCATTCGGTTTGCAA+GGG - chr8_4:51836604-51836623 Msa1332030:intron 50.0%
GCATAGCGTAAATTCGGCTG+GGG - chr8_4:51836762-51836781 Msa1332030:intron 50.0%
CTCGCCCATTTGGCATCTGT+AGG + chr8_4:51835793-51835812 None:intergenic 55.0%
! CGAGTATCTGCAAGAGGTGC+TGG + chr8_4:51835818-51835837 None:intergenic 55.0%
GTCTAATCAGTGGGTGCCTC+GGG - chr8_4:51836078-51836097 Msa1332030:CDS 55.0%
! ATTGGACCACTAGCTTCCCG+AGG + chr8_4:51836097-51836116 None:intergenic 55.0%
GGATGTGCAGCGATCACAAG+AGG + chr8_4:51836352-51836371 None:intergenic 55.0%
TCTCCACCTCCAACCGAGAA+TGG - chr8_4:51836457-51836476 Msa1332030:CDS 55.0%
ACACCATTCTCGGTTGGAGG+TGG + chr8_4:51836463-51836482 None:intergenic 55.0%
CCAACACCATTCTCGGTTGG+AGG + chr8_4:51836466-51836485 None:intergenic 55.0%
CCTCCAACCGAGAATGGTGT+TGG - chr8_4:51836463-51836482 Msa1332030:CDS 55.0%
GGACCAACACCATTCTCGGT+TGG + chr8_4:51836469-51836488 None:intergenic 55.0%
GTTGGTCCTAGCACTCCTTC+AGG - chr8_4:51836481-51836500 Msa1332030:CDS 55.0%
!! AAACCGAATGGAGGCACCAG+GGG + chr8_4:51836600-51836619 None:intergenic 55.0%
!! CAAACCGAATGGAGGCACCA+GGG + chr8_4:51836601-51836620 None:intergenic 55.0%
! GTGCCTCCATTCGGTTTGCA+AGG - chr8_4:51836603-51836622 Msa1332030:intron 55.0%
! GCCTCCATTCGGTTTGCAAG+GGG - chr8_4:51836605-51836624 Msa1332030:intron 55.0%
TCCCCTTGCAAACCGAATGG+AGG + chr8_4:51836609-51836628 None:intergenic 55.0%
GCTTCCCCTTGCAAACCGAA+TGG + chr8_4:51836612-51836631 None:intergenic 55.0%
CTCGCCCATTTGGCATCTGT+AGG + chr8_4:51835793-51835812 None:intergenic 55.0%
! CGAGTATCTGCAAGAGGTGC+TGG + chr8_4:51835818-51835837 None:intergenic 55.0%
GTCTAATCAGTGGGTGCCTC+GGG - chr8_4:51836078-51836097 Msa1332030:CDS 55.0%
! ATTGGACCACTAGCTTCCCG+AGG + chr8_4:51836097-51836116 None:intergenic 55.0%
GGATGTGCAGCGATCACAAG+AGG + chr8_4:51836352-51836371 None:intergenic 55.0%
TCTCCACCTCCAACCGAGAA+TGG - chr8_4:51836457-51836476 Msa1332030:CDS 55.0%
ACACCATTCTCGGTTGGAGG+TGG + chr8_4:51836463-51836482 None:intergenic 55.0%
CCAACACCATTCTCGGTTGG+AGG + chr8_4:51836466-51836485 None:intergenic 55.0%
CCTCCAACCGAGAATGGTGT+TGG - chr8_4:51836463-51836482 Msa1332030:CDS 55.0%
GGACCAACACCATTCTCGGT+TGG + chr8_4:51836469-51836488 None:intergenic 55.0%
GTTGGTCCTAGCACTCCTTC+AGG - chr8_4:51836481-51836500 Msa1332030:CDS 55.0%
!! AAACCGAATGGAGGCACCAG+GGG + chr8_4:51836600-51836619 None:intergenic 55.0%
!! CAAACCGAATGGAGGCACCA+GGG + chr8_4:51836601-51836620 None:intergenic 55.0%
! GTGCCTCCATTCGGTTTGCA+AGG - chr8_4:51836603-51836622 Msa1332030:intron 55.0%
! GCCTCCATTCGGTTTGCAAG+GGG - chr8_4:51836605-51836624 Msa1332030:intron 55.0%
TCCCCTTGCAAACCGAATGG+AGG + chr8_4:51836609-51836628 None:intergenic 55.0%
GCTTCCCCTTGCAAACCGAA+TGG + chr8_4:51836612-51836631 None:intergenic 55.0%
GTGTATGGAGCACGGACGGA+TGG - chr8_4:51835747-51835766 Msa1332030:three_prime_UTR 60.0%
!! GAGGCACCTGAAGGAGTGCT+AGG + chr8_4:51836490-51836509 None:intergenic 60.0%
!! GCAAACCGAATGGAGGCACC+AGG + chr8_4:51836602-51836621 None:intergenic 60.0%
CGGTTTGCAAGGGGAAGCTG+CGG - chr8_4:51836614-51836633 Msa1332030:intron 60.0%
GTGTATGGAGCACGGACGGA+TGG - chr8_4:51835747-51835766 Msa1332030:three_prime_UTR 60.0%
!! GAGGCACCTGAAGGAGTGCT+AGG + chr8_4:51836490-51836509 None:intergenic 60.0%
!! GCAAACCGAATGGAGGCACC+AGG + chr8_4:51836602-51836621 None:intergenic 60.0%
CGGTTTGCAAGGGGAAGCTG+CGG - chr8_4:51836614-51836633 Msa1332030:intron 60.0%
! GGTGCTGGAGCTCGCCCATT+TGG + chr8_4:51835803-51835822 None:intergenic 65.0%
TGGGTGCCTCGGGAAGCTAG+TGG - chr8_4:51836088-51836107 Msa1332030:CDS 65.0%
ATTGCTTGTGGTCCCGCCCC+TGG - chr8_4:51836581-51836600 Msa1332030:intron 65.0%
!! CTGGGGCGTTGCAGAGTGGA+GGG - chr8_4:51836779-51836798 Msa1332030:intron 65.0%
! GGTGCTGGAGCTCGCCCATT+TGG + chr8_4:51835803-51835822 None:intergenic 65.0%
TGGGTGCCTCGGGAAGCTAG+TGG - chr8_4:51836088-51836107 Msa1332030:CDS 65.0%
ATTGCTTGTGGTCCCGCCCC+TGG - chr8_4:51836581-51836600 Msa1332030:intron 65.0%
!! CTGGGGCGTTGCAGAGTGGA+GGG - chr8_4:51836779-51836798 Msa1332030:intron 65.0%
!! CGAATGGAGGCACCAGGGGC+GGG + chr8_4:51836596-51836615 None:intergenic 70.0%
!! CCGAATGGAGGCACCAGGGG+CGG + chr8_4:51836597-51836616 None:intergenic 70.0%
CCGCCCCTGGTGCCTCCATT+CGG - chr8_4:51836594-51836613 Msa1332030:intron 70.0%
!! TCGGCTGGGGCGTTGCAGAG+TGG - chr8_4:51836775-51836794 Msa1332030:intron 70.0%
!! GCTGGGGCGTTGCAGAGTGG+AGG - chr8_4:51836778-51836797 Msa1332030:intron 70.0%
!! CGAATGGAGGCACCAGGGGC+GGG + chr8_4:51836596-51836615 None:intergenic 70.0%
!! CCGAATGGAGGCACCAGGGG+CGG + chr8_4:51836597-51836616 None:intergenic 70.0%
CCGCCCCTGGTGCCTCCATT+CGG - chr8_4:51836594-51836613 Msa1332030:intron 70.0%
!! TCGGCTGGGGCGTTGCAGAG+TGG - chr8_4:51836775-51836794 Msa1332030:intron 70.0%
!! GCTGGGGCGTTGCAGAGTGG+AGG - chr8_4:51836778-51836797 Msa1332030:intron 70.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 51835370 51836965 51835370 ID=Msa1332030;Name=Msa1332030
chr8_4 mRNA 51835370 51836965 51835370 ID=Msa1332030-mRNA-1;Parent=Msa1332030;Name=Msa1332030-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0.33|0.25|1|1|1|4|431|201
chr8_4 exon 51835370 51835999 51835370 ID=Msa1332030-mRNA-1:exon:15426;Parent=Msa1332030-mRNA-1
chr8_4 exon 51836078 51836247 51836078 ID=Msa1332030-mRNA-1:exon:15425;Parent=Msa1332030-mRNA-1
chr8_4 exon 51836404 51836538 51836404 ID=Msa1332030-mRNA-1:exon:15424;Parent=Msa1332030-mRNA-1
chr8_4 exon 51836864 51836965 51836864 ID=Msa1332030-mRNA-1:exon:15423;Parent=Msa1332030-mRNA-1
chr8_4 CDS 51836864 51836965 51836864 ID=Msa1332030-mRNA-1:cds;Parent=Msa1332030-mRNA-1
chr8_4 CDS 51836404 51836538 51836404 ID=Msa1332030-mRNA-1:cds;Parent=Msa1332030-mRNA-1
chr8_4 CDS 51836078 51836247 51836078 ID=Msa1332030-mRNA-1:cds;Parent=Msa1332030-mRNA-1
chr8_4 CDS 51835801 51835999 51835801 ID=Msa1332030-mRNA-1:cds;Parent=Msa1332030-mRNA-1
chr8_4 three_prime_UTR 51835370 51835800 51835370 ID=Msa1332030-mRNA-1:three_prime_utr;Parent=Msa1332030-mRNA-1
Gene Sequence

>Msa1332030

ATGAAGGTCAAAACCAAGCTAAAAGGTCATCAGAAGAGAATCACTGGTCTTGCATTTTCTCATGTTCTAAATATTCTTGTATCATCTGGAGCTGACTCTCAGATGCCAAATGGGCGAGCTCCAGCACCTCTTGCAGATACTCGTGTTCAATTTCATTTAGACCAGACACATTTACTTGCTGTTCATGAAACTCAAATAGCCATATATGAGGCCCCGAAATTGGAATGCCTAAAGCAGTGGGTGCCTCGGGAAGCTAGTGGTCCAATCACACATGCTACATATTCATGTGATAGTCAATCAATATATGTAAGCTTTGAAGATGGAAGTATTGGTGTTCTTACTGCCTCAACACTCAGATTAAGATGTAGAATAAATCAAACTGCTTATCTCAATCCCAACCAAGGTGCCCTGAGAGTATATCCTCTTGTGATCGCTGCACATCCCTCCGAATCCAATCAATTTGCATTAGGACTTACAGATGGTGGAGTACATGTTCTTGAACCACTAGAGTCAGAAGGAAGGTGGGGTTCTCCACCTCCAACCGAGAATGGTGTTGGTCCTAGCACTCCTTCAGGTGCCTCTGTTTCAGAGCAACCCCAAAGGTGA
Protein sequence

>Msa1332030

MKVKTKLKGHQKRITGLAFSHVLNILVSSGADSQMPNGRAPAPLADTRVQFHLDQTHLLAVHETQIAIYEAPKLECLKQWVPREASGPITHATYSCDSQSIYVSFEDGSIGVLTASTLRLRCRINQTAYLNPNQGALRVYPLVIAAHPSESNQFALGLTDGGVHVLEPLESEGRWGSPPPTENGVGPSTPSGASVSEQPQR*