AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1333670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1333670 MtrunA17_Chr4g0069881 94.426 305 17 0 1 305 1 305 0.0 533
Msa1333670 MtrunA17_Chr2g0302001 69.832 179 52 2 1 177 1 179 2.80e-66 214
Msa1333670 MtrunA17_Chr7g0269541 34.568 324 176 13 6 305 16 327 3.75e-39 145
Msa1333670 MtrunA17_Chr8g0345281 34.416 308 155 12 40 308 68 367 4.53e-37 139
Msa1333670 MtrunA17_Chr7g0272141 29.102 323 208 6 1 304 1 321 1.30e-35 135
Msa1333670 MtrunA17_Chr1g0147051 30.946 349 172 12 4 304 50 377 4.18e-33 128
Msa1333670 MtrunA17_Chr7g0233831 31.907 257 149 5 67 305 76 324 5.75e-33 127
Msa1333670 MtrunA17_Chr7g0233801 35.628 247 137 7 75 305 83 323 6.57e-33 127
Msa1333670 MtrunA17_Chr7g0233771 35.160 219 134 4 105 318 121 336 7.09e-33 127
Msa1333670 MtrunA17_Chr8g0391281 33.103 290 153 10 44 305 34 310 1.56e-32 122
Msa1333670 MtrunA17_Chr2g0302021 36.538 208 120 6 105 304 92 295 1.88e-32 126
Msa1333670 MtrunA17_Chr7g0269511 31.642 335 181 11 8 304 11 335 2.54e-32 126
Msa1333670 MtrunA17_Chr8g0354901 31.818 286 175 7 38 305 74 357 1.44e-31 124
Msa1333670 MtrunA17_Chr8g0391311 33.103 290 153 10 44 305 36 312 7.23e-31 121
Msa1333670 MtrunA17_Chr3g0080391 31.228 285 174 9 39 305 70 350 1.02e-30 121
Msa1333670 MtrunA17_Chr7g0269531 29.904 311 159 8 41 305 40 337 8.73e-30 119
Msa1333670 MtrunA17_Chr8g0391291 31.834 289 156 10 44 304 34 309 1.26e-29 118
Msa1333670 MtrunA17_Chr4g0069861 36.574 216 110 7 105 304 83 287 2.15e-29 117
Msa1333670 MtrunA17_Chr8g0354911 32.039 309 161 12 44 305 78 384 2.70e-29 117
Msa1333670 MtrunA17_Chr1g0192331 33.866 313 163 10 11 305 11 297 2.91e-29 117
Msa1333670 MtrunA17_Chr7g0272151 26.923 338 203 10 6 304 19 351 5.87e-29 116
Msa1333670 MtrunA17_Chr4g0069841 33.333 261 149 9 54 304 41 286 5.91e-29 116
Msa1333670 MtrunA17_Chr3g0126771 29.769 346 175 13 4 304 45 367 2.31e-28 115
Msa1333670 MtrunA17_Chr4g0011791 32.526 289 158 12 29 304 18 282 3.61e-28 114
Msa1333670 MtrunA17_Chr1g0192311 28.289 304 175 12 37 309 55 346 3.50e-27 111
Msa1333670 MtrunA17_Chr1g0192281 28.289 304 175 12 37 309 55 346 3.50e-27 111
Msa1333670 MtrunA17_Chr1g0195331 30.363 303 167 8 41 304 29 326 6.69e-27 110
Msa1333670 MtrunA17_Chr7g0269521 30.392 306 162 10 41 304 37 333 7.83e-27 110
Msa1333670 MtrunA17_Chr1g0192301 29.206 315 186 13 21 309 48 351 8.41e-27 110
Msa1333670 MtrunA17_Chr1g0175661 30.357 336 180 12 7 304 10 329 8.53e-27 110
Msa1333670 MtrunA17_Chr4g0069891 30.584 291 170 10 40 304 81 365 1.40e-26 109
Msa1333670 MtrunA17_Chr4g0000121 29.206 315 186 13 21 309 30 333 1.93e-26 109
Msa1333670 MtrunA17_Chr1g0195301 30.033 303 168 8 41 304 29 326 2.75e-26 108
Msa1333670 MtrunA17_Chr1g0195321 30.033 303 168 8 41 304 29 326 2.75e-26 108
Msa1333670 MtrunA17_Chr1g0195351 30.033 303 168 8 41 304 29 326 3.06e-26 108
Msa1333670 MtrunA17_Chr4g0018371 31.449 283 168 10 40 305 36 309 3.11e-26 108
Msa1333670 MtrunA17_Chr8g0345211 31.561 301 179 11 38 318 75 368 4.17e-25 105
Msa1333670 MtrunA17_Chr4g0011771 29.747 316 174 12 4 304 2 284 8.57e-25 104
Msa1333670 MtrunA17_Chr3g0090091 29.682 283 170 8 38 305 30 298 2.65e-24 102
Msa1333670 MtrunA17_Chr1g0194091 27.203 261 158 5 87 318 90 347 3.28e-24 102
Msa1333670 MtrunA17_Chr4g0067611 28.223 287 177 10 40 304 59 338 1.42e-23 100
Msa1333670 MtrunA17_Chr1g0192321 26.899 316 179 12 31 304 47 352 1.70e-23 100
Msa1333670 MtrunA17_Chr7g0234731 29.348 276 179 8 38 305 49 316 1.98e-23 98.2
Msa1333670 MtrunA17_Chr4g0067601 27.465 284 185 8 40 305 59 339 3.95e-23 99.8
Msa1333670 MtrunA17_Chr7g0270651 28.151 238 131 6 105 306 108 341 7.38e-23 99.0
Msa1333670 MtrunA17_Chr3g0079361 28.421 285 170 11 38 305 30 297 2.07e-22 97.4
Msa1333670 MtrunA17_Chr3g0079351 28.115 313 201 10 1 305 1 297 3.36e-22 97.1
Msa1333670 MtrunA17_Chr7g0270661 27.731 238 132 6 105 306 108 341 6.68e-22 96.3
Msa1333670 MtrunA17_Chr7g0270671 27.731 238 132 6 105 306 108 341 6.68e-22 96.3
Msa1333670 MtrunA17_Chr3g0079341 30.000 280 172 10 38 305 30 297 6.70e-22 95.9
Msa1333670 MtrunA17_Chr7g0270691 28.169 284 183 9 40 304 39 320 8.98e-22 95.5
Msa1333670 MtrunA17_Chr3g0080381 29.167 312 168 11 44 305 92 400 2.97e-20 91.3
Msa1333670 MtrunA17_Chr2g0295541 26.897 290 174 12 40 305 56 331 5.50e-19 87.4
Msa1333670 MtrunA17_Chr1g0194081 26.984 252 138 8 91 304 92 335 6.57e-19 87.4
Msa1333670 MtrunA17_Chr4g0011751 32.057 209 119 8 105 304 65 259 8.36e-19 86.7
Msa1333670 MtrunA17_Chr7g0270631 25.475 263 153 6 84 306 70 329 2.24e-18 85.9
Msa1333670 MtrunA17_Chr7g0270631 28.936 235 133 7 105 306 644 877 1.23e-17 84.0
Msa1333670 MtrunA17_Chr8g0384121 27.301 326 190 10 8 305 24 330 4.47e-18 84.7
Msa1333670 MtrunA17_Chr2g0295531 22.420 281 193 7 40 305 43 313 8.52e-18 84.0
Msa1333670 MtrunA17_Chr3g0129921 27.900 319 194 11 8 305 27 330 1.81e-17 82.8
Msa1333670 MtrunA17_Chr2g0295551 25.517 290 178 11 40 305 56 331 7.33e-17 81.3
Msa1333670 MtrunA17_Chr5g0437831 42.553 94 48 2 207 295 52 144 6.40e-15 74.7
Msa1333670 MtrunA17_Chr2g0299431 40.230 87 49 2 207 291 34 119 5.48e-14 71.2
Msa1333670 MtrunA17_Chr7g0271381 23.741 278 193 6 35 304 55 321 3.17e-13 70.1
Msa1333670 MtrunA17_Chr8g0380581 40.698 86 50 1 207 291 48 133 2.12e-12 67.4
Msa1333670 MtrunA17_Chr4g0056601 30.612 196 119 7 4 184 1 194 5.20e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1333670 AT2G26440.1 55.172 290 113 5 32 304 29 318 2.84e-96 295
Msa1333670 AT4G33220.1 36.047 258 135 7 74 304 39 293 2.00e-39 145
Msa1333670 AT3G43270.1 40.455 220 107 7 105 304 80 295 6.73e-37 139
Msa1333670 AT3G05620.1 36.239 218 121 4 105 304 104 321 2.24e-34 132
Msa1333670 AT4G02320.1 34.615 260 142 6 68 305 36 289 3.79e-34 131
Msa1333670 AT4G02330.1 32.778 360 166 13 4 305 2 343 1.03e-33 130
Msa1333670 AT4G02300.1 33.074 257 146 6 69 305 53 303 2.13e-33 129
Msa1333670 AT1G02810.1 30.435 368 171 12 4 305 1 349 5.20e-32 125
Msa1333670 AT5G27870.1 28.814 295 179 9 37 308 54 340 6.82e-32 125
Msa1333670 AT1G53830.1 31.894 301 164 9 40 304 62 357 1.07e-31 124
Msa1333670 AT5G51500.1 31.959 291 174 7 30 305 28 309 2.33e-31 123
Msa1333670 AT1G11580.2 34.234 222 129 6 95 305 134 349 3.31e-31 123
Msa1333670 AT1G11580.1 34.234 222 129 6 95 305 113 328 4.37e-31 122
Msa1333670 AT3G14310.1 28.947 304 177 7 40 304 59 362 1.84e-30 121
Msa1333670 AT5G51490.1 32.364 275 168 8 43 305 37 305 2.16e-30 120
Msa1333670 AT4G33220.2 40.462 173 81 5 153 304 1 172 2.38e-30 119
Msa1333670 AT1G53840.1 32.051 312 174 11 24 304 57 361 3.60e-30 120
Msa1333670 AT3G49220.2 32.886 298 170 10 39 313 131 421 1.28e-29 117
Msa1333670 AT2G47550.1 38.034 234 110 6 104 304 98 329 3.69e-29 117
Msa1333670 AT3G05610.1 27.152 302 185 10 33 308 51 343 3.60e-28 115
Msa1333670 AT3G49220.3 32.292 288 166 9 39 304 131 411 8.79e-28 114
Msa1333670 AT3G49220.1 32.292 288 166 9 39 304 86 366 8.94e-28 113
Msa1333670 AT5G53370.2 30.634 284 177 8 39 304 110 391 3.19e-27 112
Msa1333670 AT5G53370.1 30.634 284 177 8 39 304 74 355 3.47e-27 112
Msa1333670 AT3G10710.1 30.182 275 176 8 39 304 69 336 1.38e-26 110
Msa1333670 AT3G14300.1 31.475 305 166 14 34 307 452 744 2.02e-26 110
Msa1333670 AT1G23200.1 30.667 300 165 13 44 304 38 333 5.97e-26 108
Msa1333670 AT4G33230.1 31.250 288 170 10 40 304 95 377 1.02e-25 107
Msa1333670 AT3G47400.1 30.100 299 151 12 43 305 87 363 7.02e-25 105
Msa1333670 AT2G43050.1 35.714 210 112 8 106 304 98 295 1.52e-24 103
Msa1333670 AT2G26450.1 28.671 286 171 11 40 304 109 382 2.70e-24 103
Msa1333670 AT5G04960.1 28.674 279 179 8 39 304 67 338 1.01e-23 101
Msa1333670 AT3G59010.1 36.321 212 117 8 106 304 102 308 1.28e-23 101
Msa1333670 AT5G49180.1 25.000 304 203 7 10 288 21 324 6.84e-23 99.4
Msa1333670 AT2G45220.1 31.228 285 145 12 40 304 30 283 6.94e-23 99.4
Msa1333670 AT1G11370.1 51.852 81 39 0 224 304 46 126 2.81e-22 94.7
Msa1333670 AT3G10720.2 26.129 310 174 11 44 304 83 386 2.83e-21 94.7
Msa1333670 AT5G04970.1 27.362 307 174 11 44 304 88 391 9.60e-21 93.2
Msa1333670 AT4G03930.1 30.583 206 132 4 110 307 97 299 1.23e-19 89.7
Msa1333670 AT1G11590.1 29.825 228 138 6 95 307 79 299 2.86e-19 88.6
Msa1333670 AT3G06830.1 24.832 298 197 9 39 309 50 347 6.75e-19 87.8
Msa1333670 AT3G27980.1 34.104 173 102 4 140 307 107 272 7.80e-19 87.4
Msa1333670 AT5G20860.2 29.327 208 122 6 109 304 112 306 2.56e-18 85.9
Msa1333670 AT4G00190.1 28.676 272 153 11 43 304 7 247 6.94e-18 84.3
Msa1333670 AT5G09760.2 25.926 297 176 9 38 305 69 350 8.27e-16 78.6
Msa1333670 AT3G60730.1 32.323 198 101 6 137 305 97 290 8.99e-16 78.2
Msa1333670 AT5G09760.1 25.926 297 176 9 38 305 43 324 1.06e-15 78.2
Msa1333670 AT2G47030.1 22.102 371 218 12 10 318 12 373 1.33e-15 77.8
Msa1333670 AT5G64640.1 26.384 307 166 14 37 305 90 374 2.95e-14 73.9
Msa1333670 AT3G62170.1 49.254 67 34 0 225 291 278 344 2.35e-13 70.9
Msa1333670 AT1G44980.1 45.122 82 45 0 224 305 78 159 6.72e-13 67.8
Msa1333670 AT2G47040.1 47.761 67 35 0 225 291 285 351 1.08e-12 68.9

Find 48 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCCTAGCATAGCTGTTATT+TGG 0.183966 8_4:+53867879 None:intergenic
ACATGGAAACCGGCGTTTAA+TGG 0.268525 8_4:-53868027 Msa1333670:CDS
ATCACCAAGTAGAACAATAT+TGG 0.295403 8_4:+53867797 None:intergenic
GATGAATTGAAGCAAACTTC+AGG 0.327484 8_4:+53868464 None:intergenic
GAAAGCTTAGATTCTGCTTC+GGG 0.335814 8_4:-53868152 Msa1333670:CDS
AAAGTCAATGGCTTCATTAA+TGG 0.338627 8_4:+53867905 None:intergenic
TTCTAAACATGTGTTCTTGT+TGG 0.356337 8_4:+53868172 None:intergenic
ACCTTCAGATCTGCAACTTT+AGG 0.361505 8_4:-53867720 Msa1333670:CDS
AGAAGGATGTTAATGATGTT+TGG 0.368270 8_4:+53868413 None:intergenic
TCCATCAACATCACTTCTAT+TGG 0.413607 8_4:+53867749 None:intergenic
AACATGTGTTCTTGTTGGTA+AGG 0.427562 8_4:+53868177 None:intergenic
AGAAAGCTTAGATTCTGCTT+CGG 0.433766 8_4:-53868153 Msa1333670:CDS
GAAGCTCTTACCTCAGGCTT+AGG 0.446698 8_4:+53868056 None:intergenic
CCTTTAGAAGCTCTTACCTC+AGG 0.459729 8_4:+53868050 None:intergenic
CTTCTAAAGGACATGGAAAC+CGG 0.462530 8_4:-53868037 Msa1333670:CDS
AAAACCAATATTGTTCTACT+TGG 0.463086 8_4:-53867801 Msa1333670:CDS
ATGAATTGAAGCAAACTTCA+GGG 0.465621 8_4:+53868465 None:intergenic
ATAATAATCTATGTGAAAGA+AGG 0.472192 8_4:-53867858 Msa1333670:CDS
GTTATTTGGAGCAAAGTCAA+TGG 0.493661 8_4:+53867893 None:intergenic
GCCAATAGAAGTGATGTTGA+TGG 0.496027 8_4:-53867750 Msa1333670:CDS
TTGAAGCAAACTTCAGGGTA+TGG 0.499680 8_4:+53868470 None:intergenic
TTTCAGATATTGATACTTGG+AGG 0.508364 8_4:+53868384 None:intergenic
AGCCACAACAATCACATTGT+CGG 0.515378 8_4:+53867953 None:intergenic
AATATTGTTCTACTTGGTGA+TGG 0.519268 8_4:-53867795 Msa1333670:CDS
TAATAATCTATGTGAAAGAA+GGG 0.519631 8_4:-53867857 Msa1333670:CDS
AACATCATAGAGAACAAAAG+AGG 0.532418 8_4:-53868323 Msa1333670:CDS
GTGATTGTTGTGGCTGCAGA+TGG 0.533920 8_4:-53867945 Msa1333670:CDS
TGGTTTCAGATATTGATACT+TGG 0.536909 8_4:+53868381 None:intergenic
GAATTGATTAGATAGCTTTG+TGG 0.536977 8_4:+53868361 None:intergenic
TGAAAGCTAAAGTGTGTGAA+TGG 0.538443 8_4:+53868564 None:intergenic
GTTGTGGCTGCAGATGGAAC+TGG 0.540466 8_4:-53867939 Msa1333670:CDS
TTCAGATATTGATACTTGGA+GGG 0.549415 8_4:+53868385 None:intergenic
ACATGTGTTCTTGTTGGTAA+GGG 0.551234 8_4:+53868178 None:intergenic
ACCTAAAGTTGCAGATCTGA+AGG 0.551357 8_4:+53867719 None:intergenic
ATACTTGGAGGGAATGAAGA+AGG 0.552774 8_4:+53868396 None:intergenic
GTAAGAGCTTCTAAAGGACA+TGG 0.586185 8_4:-53868044 Msa1333670:CDS
CTCCAAATAACAGCTATGCT+AGG 0.598592 8_4:-53867881 Msa1333670:CDS
GTTTAATGGATTCTCTCATG+TGG 0.602528 8_4:-53868013 Msa1333670:CDS
CCTGAGGTAAGAGCTTCTAA+AGG 0.619587 8_4:-53868050 Msa1333670:CDS
GTCAATGTTTCCTAAGCCTG+AGG 0.626799 8_4:-53868066 Msa1333670:CDS
CAATGTTGTTGTGGAAGAAG+TGG 0.630302 8_4:+53868535 None:intergenic
GTATGGAGTAGTTCTGCAGA+AGG 0.632268 8_4:+53868487 None:intergenic
CGGTGCTTTGTAAGAACCTG+CGG 0.633161 8_4:+53867973 None:intergenic
ATAGAAGTGATGTTGATGGA+TGG 0.641423 8_4:-53867746 Msa1333670:CDS
CACCGACAATGTGATTGTTG+TGG 0.664096 8_4:-53867955 Msa1333670:CDS
TGAGAGAATCCATTAAACGC+CGG 0.664960 8_4:+53868018 None:intergenic
TAAAGTTGCAGATCTGAAGG+TGG 0.679551 8_4:+53867722 None:intergenic
TGTGAAAGAAGGGATATACG+AGG 0.755347 8_4:-53867847 Msa1333670:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATAATCTATGTGAAAGA+AGG - chr8_4:53868430-53868449 Msa1333670:CDS 20.0%
!! TAATAATCTATGTGAAAGAA+GGG - chr8_4:53868431-53868450 Msa1333670:CDS 20.0%
!! ATAATAATCTATGTGAAAGA+AGG - chr8_4:53868430-53868449 Msa1333670:CDS 20.0%
!! TAATAATCTATGTGAAAGAA+GGG - chr8_4:53868431-53868450 Msa1333670:CDS 20.0%
!!! TGATCTTTTTAGTGAAGATA+AGG + chr8_4:53868026-53868045 None:intergenic 25.0%
! TCACTAAAAAGATCATTATC+TGG - chr8_4:53868031-53868050 Msa1333670:CDS 25.0%
! AAAACCAATATTGTTCTACT+TGG - chr8_4:53868487-53868506 Msa1333670:CDS 25.0%
!!! TGATCTTTTTAGTGAAGATA+AGG + chr8_4:53868026-53868045 None:intergenic 25.0%
! TCACTAAAAAGATCATTATC+TGG - chr8_4:53868031-53868050 Msa1333670:CDS 25.0%
! AAAACCAATATTGTTCTACT+TGG - chr8_4:53868487-53868506 Msa1333670:CDS 25.0%
ATGAATTGAAGCAAACTTCA+GGG + chr8_4:53867826-53867845 None:intergenic 30.0%
AGAAGGATGTTAATGATGTT+TGG + chr8_4:53867878-53867897 None:intergenic 30.0%
TTCAGATATTGATACTTGGA+GGG + chr8_4:53867906-53867925 None:intergenic 30.0%
TTTCAGATATTGATACTTGG+AGG + chr8_4:53867907-53867926 None:intergenic 30.0%
TGGTTTCAGATATTGATACT+TGG + chr8_4:53867910-53867929 None:intergenic 30.0%
! GAATTGATTAGATAGCTTTG+TGG + chr8_4:53867930-53867949 None:intergenic 30.0%
AACATCATAGAGAACAAAAG+AGG - chr8_4:53867965-53867984 Msa1333670:CDS 30.0%
TTCTAAACATGTGTTCTTGT+TGG + chr8_4:53868119-53868138 None:intergenic 30.0%
AAAGTCAATGGCTTCATTAA+TGG + chr8_4:53868386-53868405 None:intergenic 30.0%
ATCACCAAGTAGAACAATAT+TGG + chr8_4:53868494-53868513 None:intergenic 30.0%
! AATATTGTTCTACTTGGTGA+TGG - chr8_4:53868493-53868512 Msa1333670:CDS 30.0%
ATGAATTGAAGCAAACTTCA+GGG + chr8_4:53867826-53867845 None:intergenic 30.0%
AGAAGGATGTTAATGATGTT+TGG + chr8_4:53867878-53867897 None:intergenic 30.0%
TTCAGATATTGATACTTGGA+GGG + chr8_4:53867906-53867925 None:intergenic 30.0%
TTTCAGATATTGATACTTGG+AGG + chr8_4:53867907-53867926 None:intergenic 30.0%
TGGTTTCAGATATTGATACT+TGG + chr8_4:53867910-53867929 None:intergenic 30.0%
! GAATTGATTAGATAGCTTTG+TGG + chr8_4:53867930-53867949 None:intergenic 30.0%
AACATCATAGAGAACAAAAG+AGG - chr8_4:53867965-53867984 Msa1333670:CDS 30.0%
TTCTAAACATGTGTTCTTGT+TGG + chr8_4:53868119-53868138 None:intergenic 30.0%
AAAGTCAATGGCTTCATTAA+TGG + chr8_4:53868386-53868405 None:intergenic 30.0%
ATCACCAAGTAGAACAATAT+TGG + chr8_4:53868494-53868513 None:intergenic 30.0%
! AATATTGTTCTACTTGGTGA+TGG - chr8_4:53868493-53868512 Msa1333670:CDS 30.0%
TGAAAGCTAAAGTGTGTGAA+TGG + chr8_4:53867727-53867746 None:intergenic 35.0%
! TTTTGCATGCAATGTTGTTG+TGG + chr8_4:53867765-53867784 None:intergenic 35.0%
GATGAATTGAAGCAAACTTC+AGG + chr8_4:53867827-53867846 None:intergenic 35.0%
! ACATGTGTTCTTGTTGGTAA+GGG + chr8_4:53868113-53868132 None:intergenic 35.0%
AACATGTGTTCTTGTTGGTA+AGG + chr8_4:53868114-53868133 None:intergenic 35.0%
AGAAAGCTTAGATTCTGCTT+CGG - chr8_4:53868135-53868154 Msa1333670:CDS 35.0%
!! GTTTAATGGATTCTCTCATG+TGG - chr8_4:53868275-53868294 Msa1333670:CDS 35.0%
! GTTATTTGGAGCAAAGTCAA+TGG + chr8_4:53868398-53868417 None:intergenic 35.0%
ATCCTAGCATAGCTGTTATT+TGG + chr8_4:53868412-53868431 None:intergenic 35.0%
TCCATCAACATCACTTCTAT+TGG + chr8_4:53868542-53868561 None:intergenic 35.0%
! ATAGAAGTGATGTTGATGGA+TGG - chr8_4:53868542-53868561 Msa1333670:CDS 35.0%
TGAAAGCTAAAGTGTGTGAA+TGG + chr8_4:53867727-53867746 None:intergenic 35.0%
! TTTTGCATGCAATGTTGTTG+TGG + chr8_4:53867765-53867784 None:intergenic 35.0%
GATGAATTGAAGCAAACTTC+AGG + chr8_4:53867827-53867846 None:intergenic 35.0%
! ACATGTGTTCTTGTTGGTAA+GGG + chr8_4:53868113-53868132 None:intergenic 35.0%
AACATGTGTTCTTGTTGGTA+AGG + chr8_4:53868114-53868133 None:intergenic 35.0%
AGAAAGCTTAGATTCTGCTT+CGG - chr8_4:53868135-53868154 Msa1333670:CDS 35.0%
!! GTTTAATGGATTCTCTCATG+TGG - chr8_4:53868275-53868294 Msa1333670:CDS 35.0%
! GTTATTTGGAGCAAAGTCAA+TGG + chr8_4:53868398-53868417 None:intergenic 35.0%
ATCCTAGCATAGCTGTTATT+TGG + chr8_4:53868412-53868431 None:intergenic 35.0%
TCCATCAACATCACTTCTAT+TGG + chr8_4:53868542-53868561 None:intergenic 35.0%
! ATAGAAGTGATGTTGATGGA+TGG - chr8_4:53868542-53868561 Msa1333670:CDS 35.0%
CAATGTTGTTGTGGAAGAAG+TGG + chr8_4:53867756-53867775 None:intergenic 40.0%
TTGAAGCAAACTTCAGGGTA+TGG + chr8_4:53867821-53867840 None:intergenic 40.0%
ATACTTGGAGGGAATGAAGA+AGG + chr8_4:53867895-53867914 None:intergenic 40.0%
GAAAGCTTAGATTCTGCTTC+GGG - chr8_4:53868136-53868155 Msa1333670:CDS 40.0%
! GTAAGAGCTTCTAAAGGACA+TGG - chr8_4:53868244-53868263 Msa1333670:CDS 40.0%
! CTTCTAAAGGACATGGAAAC+CGG - chr8_4:53868251-53868270 Msa1333670:CDS 40.0%
TGAGAGAATCCATTAAACGC+CGG + chr8_4:53868273-53868292 None:intergenic 40.0%
AGCCACAACAATCACATTGT+CGG + chr8_4:53868338-53868357 None:intergenic 40.0%
CTCCAAATAACAGCTATGCT+AGG - chr8_4:53868407-53868426 Msa1333670:CDS 40.0%
TGTGAAAGAAGGGATATACG+AGG - chr8_4:53868441-53868460 Msa1333670:CDS 40.0%
GCCAATAGAAGTGATGTTGA+TGG - chr8_4:53868538-53868557 Msa1333670:CDS 40.0%
TAAAGTTGCAGATCTGAAGG+TGG + chr8_4:53868569-53868588 None:intergenic 40.0%
ACCTTCAGATCTGCAACTTT+AGG - chr8_4:53868568-53868587 Msa1333670:CDS 40.0%
ACCTAAAGTTGCAGATCTGA+AGG + chr8_4:53868572-53868591 None:intergenic 40.0%
CAATGTTGTTGTGGAAGAAG+TGG + chr8_4:53867756-53867775 None:intergenic 40.0%
TTGAAGCAAACTTCAGGGTA+TGG + chr8_4:53867821-53867840 None:intergenic 40.0%
ATACTTGGAGGGAATGAAGA+AGG + chr8_4:53867895-53867914 None:intergenic 40.0%
GAAAGCTTAGATTCTGCTTC+GGG - chr8_4:53868136-53868155 Msa1333670:CDS 40.0%
! GTAAGAGCTTCTAAAGGACA+TGG - chr8_4:53868244-53868263 Msa1333670:CDS 40.0%
! CTTCTAAAGGACATGGAAAC+CGG - chr8_4:53868251-53868270 Msa1333670:CDS 40.0%
TGAGAGAATCCATTAAACGC+CGG + chr8_4:53868273-53868292 None:intergenic 40.0%
AGCCACAACAATCACATTGT+CGG + chr8_4:53868338-53868357 None:intergenic 40.0%
CTCCAAATAACAGCTATGCT+AGG - chr8_4:53868407-53868426 Msa1333670:CDS 40.0%
TGTGAAAGAAGGGATATACG+AGG - chr8_4:53868441-53868460 Msa1333670:CDS 40.0%
GCCAATAGAAGTGATGTTGA+TGG - chr8_4:53868538-53868557 Msa1333670:CDS 40.0%
TAAAGTTGCAGATCTGAAGG+TGG + chr8_4:53868569-53868588 None:intergenic 40.0%
ACCTTCAGATCTGCAACTTT+AGG - chr8_4:53868568-53868587 Msa1333670:CDS 40.0%
ACCTAAAGTTGCAGATCTGA+AGG + chr8_4:53868572-53868591 None:intergenic 40.0%
! GTATGGAGTAGTTCTGCAGA+AGG + chr8_4:53867804-53867823 None:intergenic 45.0%
GTCAATGTTTCCTAAGCCTG+AGG - chr8_4:53868222-53868241 Msa1333670:CDS 45.0%
! CCTTTAGAAGCTCTTACCTC+AGG + chr8_4:53868241-53868260 None:intergenic 45.0%
CCTGAGGTAAGAGCTTCTAA+AGG - chr8_4:53868238-53868257 Msa1333670:CDS 45.0%
ACATGGAAACCGGCGTTTAA+TGG - chr8_4:53868261-53868280 Msa1333670:CDS 45.0%
TGTCAAGCAAAAATCACCGC+AGG - chr8_4:53868299-53868318 Msa1333670:CDS 45.0%
CACCGACAATGTGATTGTTG+TGG - chr8_4:53868333-53868352 Msa1333670:CDS 45.0%
! GTATGGAGTAGTTCTGCAGA+AGG + chr8_4:53867804-53867823 None:intergenic 45.0%
GTCAATGTTTCCTAAGCCTG+AGG - chr8_4:53868222-53868241 Msa1333670:CDS 45.0%
! CCTTTAGAAGCTCTTACCTC+AGG + chr8_4:53868241-53868260 None:intergenic 45.0%
CCTGAGGTAAGAGCTTCTAA+AGG - chr8_4:53868238-53868257 Msa1333670:CDS 45.0%
ACATGGAAACCGGCGTTTAA+TGG - chr8_4:53868261-53868280 Msa1333670:CDS 45.0%
TGTCAAGCAAAAATCACCGC+AGG - chr8_4:53868299-53868318 Msa1333670:CDS 45.0%
CACCGACAATGTGATTGTTG+TGG - chr8_4:53868333-53868352 Msa1333670:CDS 45.0%
GAAGCTCTTACCTCAGGCTT+AGG + chr8_4:53868235-53868254 None:intergenic 50.0%
CGGTGCTTTGTAAGAACCTG+CGG + chr8_4:53868318-53868337 None:intergenic 50.0%
GTGATTGTTGTGGCTGCAGA+TGG - chr8_4:53868343-53868362 Msa1333670:CDS 50.0%
GAAGCTCTTACCTCAGGCTT+AGG + chr8_4:53868235-53868254 None:intergenic 50.0%
CGGTGCTTTGTAAGAACCTG+CGG + chr8_4:53868318-53868337 None:intergenic 50.0%
GTGATTGTTGTGGCTGCAGA+TGG - chr8_4:53868343-53868362 Msa1333670:CDS 50.0%
GTTGTGGCTGCAGATGGAAC+TGG - chr8_4:53868349-53868368 Msa1333670:CDS 55.0%
GTTGTGGCTGCAGATGGAAC+TGG - chr8_4:53868349-53868368 Msa1333670:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 53867677 53868633 53867677 ID=Msa1333670;Name=Msa1333670
chr8_4 mRNA 53867677 53868633 53867677 ID=Msa1333670-mRNA-1;Parent=Msa1333670;Name=Msa1333670-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|318
chr8_4 exon 53867677 53868633 53867677 ID=Msa1333670-mRNA-1:exon:16583;Parent=Msa1333670-mRNA-1
chr8_4 CDS 53867677 53868633 53867677 ID=Msa1333670-mRNA-1:cds;Parent=Msa1333670-mRNA-1
Gene Sequence

>Msa1333670

ATGGCTAATTTATCTTCTAAACTCTTTCTTCTTCTCTTAACAATCTTCCATTCACACACTTTAGCTTTCAACAACACCACTTCTTCCACAACAACATTGCATGCAAAAATCTCTTCAATAAAATCCTTCTGCAGAACTACTCCATACCCTGAAGTTTGCTTCAATTCATCAAAGCTATCAATCTCAATAAACATAAATCCAAACATCATTAACATCCTTCTTCATTCCCTCCAAGTATCAATATCTGAAACCACAAAGCTATCTAATCAATTCAACAATGCTCACTCAAACATCATAGAGAACAAAAGAGGAGCTATACAAGATTGCAAAGAACTTCAACAGTCTACCTTATCTTCACTAAAAAGATCATTATCTGGAATTCATTCATCAAAATATAGAAAGTTAGTTGATGCAAGAACTTATCTCAGTGCAGCCCTTACCAACAAGAACACATGTTTAGAAAGCTTAGATTCTGCTTCGGGTACAATGAAGCAAGTATTAGTTGATTCAGTGATCAACACTTACAAGCATGTTAGTAATTCTCTGTCAATGTTTCCTAAGCCTGAGGTAAGAGCTTCTAAAGGACATGGAAACCGGCGTTTAATGGATTCTCTCATGTGGTTGTCAAGCAAAAATCACCGCAGGTTCTTACAAAGCACCGACAATGTGATTGTTGTGGCTGCAGATGGAACTGGAAACTTTAGCACCATTAATGAAGCCATTGACTTTGCTCCAAATAACAGCTATGCTAGGATAATAATCTATGTGAAAGAAGGGATATACGAGGAAAATGTTGAAATCTCAAGCAATAAAACCAATATTGTTCTACTTGGTGATGGAAGTGATCTCACTGTCATTACTGCCAATAGAAGTGATGTTGATGGATGGACCACCTTCAGATCTGCAACTTTAGGTAATCATTTAACACTTTATTTTTTAGACTATATTAAGTTTTAA
Protein sequence

>Msa1333670

MANLSSKLFLLLLTIFHSHTLAFNNTTSSTTTLHAKISSIKSFCRTTPYPEVCFNSSKLSISININPNIINILLHSLQVSISETTKLSNQFNNAHSNIIENKRGAIQDCKELQQSTLSSLKRSLSGIHSSKYRKLVDARTYLSAALTNKNTCLESLDSASGTMKQVLVDSVINTYKHVSNSLSMFPKPEVRASKGHGNRRLMDSLMWLSSKNHRRFLQSTDNVIVVAADGTGNFSTINEAIDFAPNNSYARIIIYVKEGIYEENVEISSNKTNIVLLGDGSDLTVITANRSDVDGWTTFRSATLGNHLTLYFLDYIKF*