Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1336280 | AES66576.1 | 96.512 | 86 | 3 | 0 | 1 | 86 | 1 | 86 | 6.40e-51 | 164 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1336280 | G7ITF8 | 96.512 | 86 | 3 | 0 | 1 | 86 | 1 | 86 | 3.06e-51 | 164 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0423020 | Msa1336280 | 0.806194 | 9.257829e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1336280 | MtrunA17_Chr2g0315541 | 96.512 | 86 | 3 | 0 | 1 | 86 | 1 | 86 | 5.89e-55 | 164 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACGAGAACTCTTCCATTTC+TGG | 0.119625 | tig0000897:-7710 | Msa1336280:intergenic |
| CTTCCACTTCCATGAATATT+TGG | 0.151030 | tig0000897:-7670 | Msa1336280:intergenic |
| ACAAGATGTGTCATCTTTAA+AGG | 0.229347 | tig0000897:+7609 | Msa1336280:CDS |
| ACGAAAGATTCTCATGAAAT+TGG | 0.298007 | tig0000897:+7582 | Msa1336280:CDS |
| TTATTAGTAACCTCAATTAA+AGG | 0.298722 | tig0000897:-7640 | Msa1336280:intergenic |
| AATAATATGCCAAATATTCA+TGG | 0.351817 | tig0000897:+7661 | Msa1336280:CDS |
| TAGTGTCCAGCACCGGGGCT+AGG | 0.370157 | tig0000897:-7742 | Msa1336280:intergenic |
| GTTGCTCATCTTGCCAGAAA+TGG | 0.387597 | tig0000897:+7697 | Msa1336280:CDS |
| ACTCATCACTCTCCTTGTCT+TGG | 0.440018 | tig0000897:+7528 | Msa1336280:CDS |
| CAAGATGTGTCATCTTTAAA+GGG | 0.454793 | tig0000897:+7610 | Msa1336280:CDS |
| ATGCCAAATATTCATGGAAG+TGG | 0.470691 | tig0000897:+7667 | Msa1336280:CDS |
| TCTAACCCTAGCCCCGGTGC+TGG | 0.477835 | tig0000897:+7736 | Msa1336280:CDS |
| TATCATTAGTGTCCAGCACC+GGG | 0.519217 | tig0000897:-7748 | Msa1336280:intergenic |
| TAGTAACCTCAATTAAAGGT+AGG | 0.523999 | tig0000897:-7636 | Msa1336280:intergenic |
| AGTGTCCAGCACCGGGGCTA+GGG | 0.533590 | tig0000897:-7741 | Msa1336280:intergenic |
| ATATCATTAGTGTCCAGCAC+CGG | 0.534826 | tig0000897:-7749 | Msa1336280:intergenic |
| TTTCGTGCATCTATAGAAGA+TGG | 0.541893 | tig0000897:-7565 | Msa1336280:intergenic |
| AGTAACCTCAATTAAAGGTA+GGG | 0.546397 | tig0000897:-7635 | Msa1336280:intergenic |
| GTGTCGTCTAACCCTAGCCC+CGG | 0.595502 | tig0000897:+7730 | Msa1336280:CDS |
| ATGAATAAATTACCAAGACA+AGG | 0.646639 | tig0000897:-7540 | Msa1336270:intergenic |
| ATCATTAGTGTCCAGCACCG+GGG | 0.719551 | tig0000897:-7747 | Msa1336270:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATTAGTAACCTCAATTAA+AGG | - | tig0000897:7643-7662 | Msa1336280:intergenic | 20.0% |
| !! | AATAATATGCCAAATATTCA+TGG | + | tig0000897:7661-7680 | Msa1336280:CDS | 20.0% |
| ! | ATGAATAAATTACCAAGACA+AGG | - | tig0000897:7543-7562 | Msa1336280:intergenic | 25.0% |
| ACGAAAGATTCTCATGAAAT+TGG | + | tig0000897:7582-7601 | Msa1336280:CDS | 30.0% | |
| ACAAGATGTGTCATCTTTAA+AGG | + | tig0000897:7609-7628 | Msa1336280:CDS | 30.0% | |
| CAAGATGTGTCATCTTTAAA+GGG | + | tig0000897:7610-7629 | Msa1336280:CDS | 30.0% | |
| AGTAACCTCAATTAAAGGTA+GGG | - | tig0000897:7638-7657 | Msa1336280:intergenic | 30.0% | |
| TAGTAACCTCAATTAAAGGT+AGG | - | tig0000897:7639-7658 | Msa1336280:intergenic | 30.0% | |
| TTTCGTGCATCTATAGAAGA+TGG | - | tig0000897:7568-7587 | Msa1336280:intergenic | 35.0% | |
| ATGCCAAATATTCATGGAAG+TGG | + | tig0000897:7667-7686 | Msa1336280:CDS | 35.0% | |
| CTTCCACTTCCATGAATATT+TGG | - | tig0000897:7673-7692 | Msa1336280:intergenic | 35.0% | |
| ACTCATCACTCTCCTTGTCT+TGG | + | tig0000897:7528-7547 | Msa1336280:CDS | 45.0% | |
| GGGTACCCTACCTTTAATTG+AGG | + | tig0000897:7630-7649 | Msa1336280:CDS | 45.0% | |
| GTTGCTCATCTTGCCAGAAA+TGG | + | tig0000897:7697-7716 | Msa1336280:CDS | 45.0% | |
| CACGAGAACTCTTCCATTTC+TGG | - | tig0000897:7713-7732 | Msa1336280:intergenic | 45.0% | |
| GTGTCGTCTAACCCTAGCCC+CGG | + | tig0000897:7730-7749 | Msa1336280:CDS | 60.0% | |
| TCTAACCCTAGCCCCGGTGC+TGG | + | tig0000897:7736-7755 | Msa1336280:CDS | 65.0% | |
| ! | AGTGTCCAGCACCGGGGCTA+GGG | - | tig0000897:7744-7763 | Msa1336280:intergenic | 65.0% |
| ! | TAGTGTCCAGCACCGGGGCT+AGG | - | tig0000897:7745-7764 | Msa1336280:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0000897 | gene | 7505 | 7765 | 7505 | ID=Msa1336280;Name=Msa1336280 |
| tig0000897 | mRNA | 7505 | 7765 | 7505 | ID=Msa1336280-mRNA-1;Parent=Msa1336280;Name=Msa1336280-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|86 |
| tig0000897 | exon | 7505 | 7765 | 7505 | ID=Msa1336280-mRNA-1:exon:661;Parent=Msa1336280-mRNA-1 |
| tig0000897 | CDS | 7505 | 7765 | 7505 | ID=Msa1336280-mRNA-1:cds;Parent=Msa1336280-mRNA-1 |
| Gene Sequence |
| Protein sequence |
