AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1347300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1347300 MtrunA17_Chr8g0337631 93.191 235 16 0 1 235 1 235 2.41e-159 440
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1347300 AT3G07525.1 55.046 218 94 4 14 228 9 225 9.07e-75 226
Msa1347300 AT3G07525.2 54.338 219 95 4 14 228 9 226 5.02e-74 224

Find 73 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAACAATATCTAGTTTCT+TGG 0.247026 tig0009650:-7632 Msa1347300:CDS
TACCATGGCCTGTTCAAATA+TGG 0.250385 tig0009650:+7732 None:intergenic
CCCTATTCCAAAACATCATT+TGG 0.265019 tig0009650:-9727 Msa1347300:CDS
GTTTCTTGGTTCTCGGTTGT+AGG 0.310367 tig0009650:-7618 Msa1347300:CDS
GGAGTACCCAAATGATGTTT+TGG 0.360930 tig0009650:+9720 None:intergenic
TTCTCAATTAAAATATAGAT+TGG 0.370767 tig0009650:-7199 Msa1347300:three_prime_UTR
TTGAGTTATAAATGTCCATT+TGG 0.376190 tig0009650:+8600 None:intergenic
ATATCTAGTTTCTTGGTTCT+CGG 0.387246 tig0009650:-7625 Msa1347300:CDS
ATATTTCATGCAAATGTAGA+AGG 0.393608 tig0009650:+7357 None:intergenic
CCTCCATCACATCTAATGTT+AGG 0.397510 tig0009650:+7490 None:intergenic
TGTAAATGGTAGAAAGTTGC+AGG 0.400707 tig0009650:-7445 Msa1347300:three_prime_UTR
TAGTGATTTACTTCATTCTC+TGG 0.400799 tig0009650:+9104 None:intergenic
GCCAATTAGTGAAATTGAAA+AGG 0.404414 tig0009650:-8659 Msa1347300:CDS
CTTCCTTTCCTCCATGGCTA+TGG 0.404935 tig0009650:-9752 Msa1347300:CDS
GAAATTGAAAAGGACCTTCC+TGG 0.411204 tig0009650:-8649 Msa1347300:CDS
ATGTTTCGATTAGAAATGAT+TGG 0.425510 tig0009650:-7393 Msa1347300:three_prime_UTR
AGGAACACCCATATTTGAAC+AGG 0.430017 tig0009650:-7740 Msa1347300:CDS
TAAAACTCCTCTGGCAATGC+TGG 0.434038 tig0009650:-7580 Msa1347300:CDS
GGTGTGTTTGCAGTTGTAAA+TGG 0.442275 tig0009650:-7459 Msa1347300:three_prime_UTR
TAGATGTGATGGAGGAAGGA+AGG 0.444280 tig0009650:-7482 Msa1347300:three_prime_UTR
CTATAGTTCAGTTATTTAAG+TGG 0.448028 tig0009650:-10033 Msa1347300:intron
CCTATTCCAAAACATCATTT+GGG 0.455954 tig0009650:-9726 Msa1347300:CDS
AATATTATCGTTAATCATAT+CGG 0.456372 tig0009650:-7339 Msa1347300:three_prime_UTR
TCGGTTGTAGGGCAAGTTGT+TGG 0.460338 tig0009650:-7606 Msa1347300:CDS
TCAATCCTTCCTTTCCTCCA+TGG 0.463459 tig0009650:-9758 Msa1347300:CDS
GTGTTGGTTCATTGTTCAAT+CGG 0.464738 tig0009650:+10059 None:intergenic
TTTCTTGGTTCTCGGTTGTA+GGG 0.476095 tig0009650:-7617 Msa1347300:CDS
ATCGGTTGATCCGTCCAGAT+TGG 0.480841 tig0009650:+10077 None:intergenic
TGTTGGCCTTAAAACTCCTC+TGG 0.481319 tig0009650:-7589 Msa1347300:CDS
GAAAAGTGAACATACCACTA+TGG 0.482631 tig0009650:+9021 None:intergenic
ATCCTTGTGGGACGAGCGAA+TGG 0.492979 tig0009650:-7701 Msa1347300:CDS
AAGATGTCAATGGTATCAAA+AGG 0.497808 tig0009650:+7229 None:intergenic
GGATTTCAAAGAAGCCGTTA+CGG 0.506459 tig0009650:-9862 Msa1347300:CDS
AACTAAAGTTGCATCATCAA+AGG 0.507737 tig0009650:+9255 None:intergenic
AATTGCTATTAGAATCCAAA+TGG 0.507906 tig0009650:-8615 Msa1347300:CDS
CAGAAAATGTAGACGCAGAG+AGG 0.517227 tig0009650:+9796 None:intergenic
AGATGTGATGGAGGAAGGAA+GGG 0.519115 tig0009650:-7481 Msa1347300:three_prime_UTR
ACATCATTTGGGTACTCCTA+AGG 0.527633 tig0009650:-9715 Msa1347300:intron
ACTAAAGTTGCATCATCAAA+GGG 0.528076 tig0009650:+9256 None:intergenic
TCAATTTCACTAATTGGCAA+AGG 0.528462 tig0009650:+8664 None:intergenic
GAAATATACAAAGATGTCAA+TGG 0.545068 tig0009650:+7219 None:intergenic
ACCATGGCCTGTTCAAATAT+GGG 0.545419 tig0009650:+7733 None:intergenic
AACACTGGAACTCTATATGA+AGG 0.545483 tig0009650:+9059 None:intergenic
ACATTAGATGTGATGGAGGA+AGG 0.556713 tig0009650:-7486 Msa1347300:three_prime_UTR
GGTACAAGTTACATCCTTGT+GGG 0.560064 tig0009650:-7713 Msa1347300:CDS
CGGACAAAATCCCTTGGGAC+GGG 0.567953 tig0009650:-9842 Msa1347300:CDS
GGAATAGGGGTCCATAGCCA+TGG 0.572423 tig0009650:+9741 None:intergenic
CATAGCCATGGAGGAAAGGA+AGG 0.572655 tig0009650:+9753 None:intergenic
ACCCATATTTGAACAGGCCA+TGG 0.576828 tig0009650:-7734 Msa1347300:CDS
GGTCCATAGCCATGGAGGAA+AGG 0.578115 tig0009650:+9749 None:intergenic
TGACCTAACATTAGATGTGA+TGG 0.580102 tig0009650:-7493 Msa1347300:three_prime_UTR
TGGATGAAACTGCTCTTCCA+TGG 0.583720 tig0009650:-7681 Msa1347300:CDS
AGCAATTCTGCAGAATGACC+AGG 0.585333 tig0009650:+8631 None:intergenic
TACTTCCGTTCGTACCATAG+TGG 0.587737 tig0009650:-9035 Msa1347300:intron
AGTTTCAAATGATTCTTAGA+CGG 0.594907 tig0009650:-7550 Msa1347300:exon
CAAGGATGTAACTTGTACCA+TGG 0.595400 tig0009650:+7717 None:intergenic
CCGTTACGGACAAAATCCCT+TGG 0.595933 tig0009650:-9848 Msa1347300:CDS
AACTGAACTATAGAGAGTGT+TGG 0.599368 tig0009650:+10043 None:intergenic
AAGAGATAGTCCCGTCCCAA+GGG 0.600956 tig0009650:+9832 None:intergenic
CGTTACGGACAAAATCCCTT+GGG 0.605773 tig0009650:-9847 Msa1347300:CDS
GATGTGATGGAGGAAGGAAG+GGG 0.611511 tig0009650:-7480 Msa1347300:three_prime_UTR
TGGTACAAGTTACATCCTTG+TGG 0.612942 tig0009650:-7714 Msa1347300:CDS
ACGGACAAAATCCCTTGGGA+CGG 0.615562 tig0009650:-9843 Msa1347300:CDS
AGAAAATGTAGACGCAGAGA+GGG 0.623229 tig0009650:+9797 None:intergenic
CCTAACATTAGATGTGATGG+AGG 0.623510 tig0009650:-7490 Msa1347300:three_prime_UTR
TATTTCATGCAAATGTAGAA+GGG 0.630843 tig0009650:+7358 None:intergenic
ACATACCACTATGGTACGAA+CGG 0.648308 tig0009650:+9030 None:intergenic
TACGAACGGAAGTACAACAC+TGG 0.657678 tig0009650:+9044 None:intergenic
TATTCAAAGATGTATCACCA+TGG 0.691074 tig0009650:+7664 None:intergenic
ACGTTGTCCAGCATTGCCAG+AGG 0.697106 tig0009650:+7573 None:intergenic
ATAGGGGTCCATAGCCATGG+AGG 0.701517 tig0009650:+9744 None:intergenic
GTGTCTTGTCAAATCAAGCG+AGG 0.706853 tig0009650:-9565 Msa1347300:intron
ATTCTGCAGAATGACCAGGA+AGG 0.761296 tig0009650:+8635 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAAATGTCATATTTTTAT+TGG + tig0009650:8911-8930 None:intergenic 10.0%
!! TTTGTTTATATATAATGAAA+TGG - tig0009650:9358-9377 Msa1347300:intron 10.0%
!! TTGTTTATATATAATGAAAT+GGG - tig0009650:9359-9378 Msa1347300:intron 10.0%
!!! TATAGATATTTTTTTTTGAA+AGG - tig0009650:9955-9974 Msa1347300:intron 10.0%
!!! TAATTTATTTTGTTCAAACT+AGG + tig0009650:7281-7300 None:intergenic 15.0%
!! ACAAAAAATAGTTAAAACAT+AGG + tig0009650:8740-8759 None:intergenic 15.0%
!! AATATTATCGTTAATCATAT+CGG - tig0009650:9932-9951 Msa1347300:intron 15.0%
!! TTCTCAATTAAAATATAGAT+TGG - tig0009650:10072-10091 Msa1347300:five_prime_UTR 15.0%
!! ATGCAGATAAAAAAATATTC+AGG + tig0009650:7640-7659 None:intergenic 20.0%
!! TGAAACTTATATAAAATCAG+TGG + tig0009650:7814-7833 None:intergenic 20.0%
!! TCTATATTCAATAGCATAAA+TGG + tig0009650:8423-8442 None:intergenic 20.0%
!!! ACAAATAATTTTGTTACTGT+TGG - tig0009650:8792-8811 Msa1347300:intron 20.0%
!! TTTAGATTCAAATTATGAAC+CGG - tig0009650:8830-8849 Msa1347300:intron 20.0%
!! TATATAAACAAACAAAAGCA+AGG + tig0009650:9350-9369 None:intergenic 20.0%
!! TAATAAACTAACTGATGATA+AGG + tig0009650:9458-9477 None:intergenic 20.0%
!!! AAACCATTTAGTTTAATGTA+TGG + tig0009650:9495-9514 None:intergenic 20.0%
!!! GTTTCAATTTTATATCTTTC+AGG - tig0009650:9511-9530 Msa1347300:intron 20.0%
! CTATAGTTCAGTTATTTAAG+TGG - tig0009650:7238-7257 Msa1347300:three_prime_UTR 25.0%
! TTATTTAAGTGGTATGATCA+CGG - tig0009650:7249-7268 Msa1347300:three_prime_UTR 25.0%
! AAATTAGTAGAACCATAACT+CGG - tig0009650:7295-7314 Msa1347300:intron 25.0%
!!! GTCGGTAATGTTTTAAAAAT+TGG + tig0009650:7345-7364 None:intergenic 25.0%
! AAATACTCCAAAAATTGTGT+CGG + tig0009650:7363-7382 None:intergenic 25.0%
!!! TAGTGTAAAGCTGATTTTTA+TGG - tig0009650:7742-7761 Msa1347300:intron 25.0%
! TGAATTGTAATCTTCATTAC+AGG - tig0009650:7930-7949 Msa1347300:intron 25.0%
! TTAGTTTAGGAATACCAATT+TGG + tig0009650:8049-8068 None:intergenic 25.0%
!! GGGATAAAGTAAATTAGTTT+AGG + tig0009650:8062-8081 None:intergenic 25.0%
! ATTATGCAAGTAGTTTGATA+AGG + tig0009650:8124-8143 None:intergenic 25.0%
! GTTTATGGTAAATAGCTTAA+TGG - tig0009650:8287-8306 Msa1347300:intron 25.0%
!!! ACAATCAAGTTTTATAACCA+AGG - tig0009650:8329-8348 Msa1347300:intron 25.0%
! TTATAACCAAGGTTTCATTA+TGG - tig0009650:8340-8359 Msa1347300:intron 25.0%
! TTTCATTATGGTTGTTTGAT+TGG - tig0009650:8352-8371 Msa1347300:intron 25.0%
! ACTTCAAATTCAAACAATCT+AGG + tig0009650:8380-8399 None:intergenic 25.0%
! AATAGCATAAATGGTGTAAT+AGG + tig0009650:8414-8433 None:intergenic 25.0%
! ATATCGTCATTAGATATGAA+CGG + tig0009650:8504-8523 None:intergenic 25.0%
! ATACTGTTTGATTATGTAGA+TGG - tig0009650:8580-8599 Msa1347300:intron 25.0%
!! TCCTTTTCAATTTCACTAAT+TGG + tig0009650:8616-8635 None:intergenic 25.0%
!! AATTGCTATTAGAATCCAAA+TGG - tig0009650:8656-8675 Msa1347300:CDS 25.0%
! TTGAGTTATAAATGTCCATT+TGG + tig0009650:8674-8693 None:intergenic 25.0%
! AACATAGGCATTTAAAGAAA+AGG + tig0009650:8725-8744 None:intergenic 25.0%
! TTCTTATATTGAACTCTGTA+TGG + tig0009650:9207-9226 None:intergenic 25.0%
! AAATGGGATTCAGAAATAAA+GGG - tig0009650:9375-9394 Msa1347300:intron 25.0%
! TAGTTTAATGTATGGAAGAA+TGG + tig0009650:9487-9506 None:intergenic 25.0%
! CTTCCATACATTAAACTAAA+TGG - tig0009650:9489-9508 Msa1347300:intron 25.0%
!! TTGAACAATATCTAGTTTCT+TGG - tig0009650:9639-9658 Msa1347300:intron 25.0%
! AGTTTCAAATGATTCTTAGA+CGG - tig0009650:9721-9740 Msa1347300:CDS 25.0%
! ATGTTTCGATTAGAAATGAT+TGG - tig0009650:9878-9897 Msa1347300:intron 25.0%
! TATTTCATGCAAATGTAGAA+GGG + tig0009650:9916-9935 None:intergenic 25.0%
! ATATTTCATGCAAATGTAGA+AGG + tig0009650:9917-9936 None:intergenic 25.0%
! GAAATATACAAAGATGTCAA+TGG + tig0009650:10055-10074 None:intergenic 25.0%
AAATTGGATCCACATTAAAC+TGG + tig0009650:7329-7348 None:intergenic 30.0%
! AACATTACCGACACAATTTT+TGG - tig0009650:7353-7372 Msa1347300:three_prime_UTR 30.0%
!! TTGAGCTTCTTAGTTTTTGT+AGG - tig0009650:7381-7400 Msa1347300:three_prime_UTR 30.0%
!! TGAGCTTCTTAGTTTTTGTA+GGG - tig0009650:7382-7401 Msa1347300:three_prime_UTR 30.0%
!! TCTTAGTTTTTGTAGGGATA+TGG - tig0009650:7388-7407 Msa1347300:three_prime_UTR 30.0%
!!! CAAATGATGTTTTGGAATAG+GGG + tig0009650:7546-7565 None:intergenic 30.0%
!!! CCAAATGATGTTTTGGAATA+GGG + tig0009650:7547-7566 None:intergenic 30.0%
CCTATTCCAAAACATCATTT+GGG - tig0009650:7545-7564 Msa1347300:exon 30.0%
ACTAAAGTTGCATCATCAAA+GGG + tig0009650:8018-8037 None:intergenic 30.0%
AACTAAAGTTGCATCATCAA+AGG + tig0009650:8019-8038 None:intergenic 30.0%
!! TGATGCAACTTTAGTTTGTA+TGG - tig0009650:8023-8042 Msa1347300:intron 30.0%
!! TTTAGTTTGTATGGCCAAAT+TGG - tig0009650:8032-8051 Msa1347300:intron 30.0%
AGAATTCAGACTTATGGTAT+GGG + tig0009650:8082-8101 None:intergenic 30.0%
TAGTGATTTACTTCATTCTC+TGG + tig0009650:8170-8189 None:intergenic 30.0%
!! TATGTTCACTTTTCTATCTG+TGG - tig0009650:8259-8278 Msa1347300:intron 30.0%
! CTATCTGTGGAATTTGTTTA+TGG - tig0009650:8272-8291 Msa1347300:intron 30.0%
AAACAACCATAATGAAACCT+TGG + tig0009650:8349-8368 None:intergenic 30.0%
! TTTGAATTTGAAGTGATCCT+CGG - tig0009650:8386-8405 Msa1347300:intron 30.0%
TCAATTTCACTAATTGGCAA+AGG + tig0009650:8610-8629 None:intergenic 30.0%
GCCAATTAGTGAAATTGAAA+AGG - tig0009650:8612-8631 Msa1347300:CDS 30.0%
! AATTTTGTTACTGTTGGATG+TGG - tig0009650:8798-8817 Msa1347300:intron 30.0%
GAAATGGGATTCAGAAATAA+AGG - tig0009650:9374-9393 Msa1347300:intron 30.0%
TAAGGTGTGAAGATTAATTG+TGG + tig0009650:9440-9459 None:intergenic 30.0%
TATTCAAAGATGTATCACCA+TGG + tig0009650:9610-9629 None:intergenic 30.0%
! ATATCTAGTTTCTTGGTTCT+CGG - tig0009650:9646-9665 Msa1347300:intron 30.0%
ATACAGATATTACAGATGTG+TGG + tig0009650:10012-10031 None:intergenic 30.0%
AAGATGTCAATGGTATCAAA+AGG + tig0009650:10045-10064 None:intergenic 30.0%
!! GTGTTGGTTCATTGTTCAAT+CGG + tig0009650:7215-7234 None:intergenic 35.0%
! AACTGAACTATAGAGAGTGT+TGG + tig0009650:7231-7250 None:intergenic 35.0%
CCCTATTCCAAAACATCATT+TGG - tig0009650:7544-7563 Msa1347300:exon 35.0%
!!! CCCAAATGATGTTTTGGAAT+AGG + tig0009650:7548-7567 None:intergenic 35.0%
TTTATGGATGTGTGCATGTT+TGG - tig0009650:7758-7777 Msa1347300:intron 35.0%
! AGCTTTTCTCAAAGTTACAG+CGG - tig0009650:7840-7859 Msa1347300:intron 35.0%
TAAGTCCCATAAACTTCACT+CGG + tig0009650:7909-7928 None:intergenic 35.0%
GAGAATTCAGACTTATGGTA+TGG + tig0009650:8083-8102 None:intergenic 35.0%
AACACTGGAACTCTATATGA+AGG + tig0009650:8215-8234 None:intergenic 35.0%
GAAAAGTGAACATACCACTA+TGG + tig0009650:8253-8272 None:intergenic 35.0%
AATTCCGAAAGTAGATCTTC+CGG + tig0009650:8852-8871 None:intergenic 35.0%
CAAATGATAACAGAAGTGAC+TGG - tig0009650:9013-9032 Msa1347300:intron 35.0%
CATTCTCTTTGTCAACTCTA+TGG + tig0009650:9048-9067 None:intergenic 35.0%
CATAGAGTTGACAAAGAGAA+TGG - tig0009650:9046-9065 Msa1347300:CDS 35.0%
!! TTCACTTTTGTATCGAAGCA+CGG - tig0009650:9244-9263 Msa1347300:intron 35.0%
AACAAACAAAAGCAAGGATC+AGG + tig0009650:9344-9363 None:intergenic 35.0%
TGACCTAACATTAGATGTGA+TGG - tig0009650:9778-9797 Msa1347300:CDS 35.0%
TGTAAATGGTAGAAAGTTGC+AGG - tig0009650:9826-9845 Msa1347300:CDS 35.0%
GAAGTCTTGCCAGTTTAATG+TGG - tig0009650:7317-7336 Msa1347300:three_prime_UTR 40.0%
GGATTTCAAAGAAGCCGTTA+CGG - tig0009650:7409-7428 Msa1347300:three_prime_UTR 40.0%
AGAAAATGTAGACGCAGAGA+GGG + tig0009650:7477-7496 None:intergenic 40.0%
!! GGAGTACCCAAATGATGTTT+TGG + tig0009650:7554-7573 None:intergenic 40.0%
ACATCATTTGGGTACTCCTA+AGG - tig0009650:7556-7575 Msa1347300:CDS 40.0%
TATGACAAAGCAGTCACCTT+AGG + tig0009650:7575-7594 None:intergenic 40.0%
AAGGTGACTGCTTTGTCATA+TGG - tig0009650:7575-7594 Msa1347300:CDS 40.0%
GTATCCTTCAACCTGACATA+GGG + tig0009650:7669-7688 None:intergenic 40.0%
AGTATCCTTCAACCTGACAT+AGG + tig0009650:7670-7689 None:intergenic 40.0%
ATGCACCGAGTGAAGTTTAT+GGG - tig0009650:7901-7920 Msa1347300:intron 40.0%
CTGCAGAGAATTCAGACTTA+TGG + tig0009650:8088-8107 None:intergenic 40.0%
TGCATTTCTTCCTTCCTACT+AGG - tig0009650:8143-8162 Msa1347300:intron 40.0%
ACATACCACTATGGTACGAA+CGG + tig0009650:8244-8263 None:intergenic 40.0%
AAATGGTGTAATAGGATCCG+AGG + tig0009650:8406-8425 None:intergenic 40.0%
GAAATTGAAAAGGACCTTCC+TGG - tig0009650:8622-8641 Msa1347300:CDS 40.0%
TGAACCGGAAGATCTACTTT+CGG - tig0009650:8845-8864 Msa1347300:intron 40.0%
AACAAAAGCAAGGATCAGGT+TGG + tig0009650:9340-9359 None:intergenic 40.0%
AGGAACACCCATATTTGAAC+AGG - tig0009650:9531-9550 Msa1347300:intron 40.0%
ACCATGGCCTGTTCAAATAT+GGG + tig0009650:9541-9560 None:intergenic 40.0%
TACCATGGCCTGTTCAAATA+TGG + tig0009650:9542-9561 None:intergenic 40.0%
! CAAGGATGTAACTTGTACCA+TGG + tig0009650:9557-9576 None:intergenic 40.0%
TGGTACAAGTTACATCCTTG+TGG - tig0009650:9557-9576 Msa1347300:intron 40.0%
GGTACAAGTTACATCCTTGT+GGG - tig0009650:9558-9577 Msa1347300:intron 40.0%
! TTTCTTGGTTCTCGGTTGTA+GGG - tig0009650:9654-9673 Msa1347300:intron 40.0%
CCTCCATCACATCTAATGTT+AGG + tig0009650:9784-9803 None:intergenic 40.0%
CCTAACATTAGATGTGATGG+AGG - tig0009650:9781-9800 Msa1347300:CDS 40.0%
! ACATTAGATGTGATGGAGGA+AGG - tig0009650:9785-9804 Msa1347300:CDS 40.0%
GGTGTGTTTGCAGTTGTAAA+TGG - tig0009650:9812-9831 Msa1347300:CDS 40.0%
! CCAAGGGATTTTGTCCGTAA+CGG + tig0009650:7426-7445 None:intergenic 45.0%
CGTTACGGACAAAATCCCTT+GGG - tig0009650:7424-7443 Msa1347300:three_prime_UTR 45.0%
CAGAAAATGTAGACGCAGAG+AGG + tig0009650:7478-7497 None:intergenic 45.0%
! CTGCGTCTACATTTTCTGAC+AGG - tig0009650:7480-7499 Msa1347300:three_prime_UTR 45.0%
! CGTCTACATTTTCTGACAGG+TGG - tig0009650:7483-7502 Msa1347300:three_prime_UTR 45.0%
TCAATCCTTCCTTTCCTCCA+TGG - tig0009650:7513-7532 Msa1347300:three_prime_UTR 45.0%
CAGATGCTAAGCATGGAACA+AGG + tig0009650:7616-7635 None:intergenic 45.0%
TTCAGGACAGATGCTAAGCA+TGG + tig0009650:7623-7642 None:intergenic 45.0%
GTGTCTTGTCAAATCAAGCG+AGG - tig0009650:7706-7725 Msa1347300:CDS 45.0%
GCATGTTTGGATTGACAGTG+AGG + tig0009650:7885-7904 None:intergenic 45.0%
!! AACTTCACTCGGTGCATGTT+TGG + tig0009650:7898-7917 None:intergenic 45.0%
CATGCACCGAGTGAAGTTTA+TGG - tig0009650:7900-7919 Msa1347300:intron 45.0%
ATTCTCTGGACCTAGTAGGA+AGG + tig0009650:8156-8175 None:intergenic 45.0%
CTTCATTCTCTGGACCTAGT+AGG + tig0009650:8160-8179 None:intergenic 45.0%
! TACGAACGGAAGTACAACAC+TGG + tig0009650:8230-8249 None:intergenic 45.0%
TACTTCCGTTCGTACCATAG+TGG - tig0009650:8236-8255 Msa1347300:intron 45.0%
ATTCTGCAGAATGACCAGGA+AGG + tig0009650:8639-8658 None:intergenic 45.0%
AGCAATTCTGCAGAATGACC+AGG + tig0009650:8643-8662 None:intergenic 45.0%
TGTCAACTCTATGGCCACAT+TGG + tig0009650:9039-9058 None:intergenic 45.0%
GAGTTGACAAAGAGAATGGC+TGG - tig0009650:9050-9069 Msa1347300:CDS 45.0%
ACCCATATTTGAACAGGCCA+TGG - tig0009650:9537-9556 Msa1347300:intron 45.0%
TGGATGAAACTGCTCTTCCA+TGG - tig0009650:9590-9609 Msa1347300:CDS 45.0%
! GTTTCTTGGTTCTCGGTTGT+AGG - tig0009650:9653-9672 Msa1347300:intron 45.0%
!! TGTTGGCCTTAAAACTCCTC+TGG - tig0009650:9682-9701 Msa1347300:intron 45.0%
! GCATTGCCAGAGGAGTTTTA+AGG + tig0009650:9691-9710 None:intergenic 45.0%
TAAAACTCCTCTGGCAATGC+TGG - tig0009650:9691-9710 Msa1347300:intron 45.0%
! TAGATGTGATGGAGGAAGGA+AGG - tig0009650:9789-9808 Msa1347300:CDS 45.0%
! AGATGTGATGGAGGAAGGAA+GGG - tig0009650:9790-9809 Msa1347300:CDS 45.0%
ATCGGTTGATCCGTCCAGAT+TGG + tig0009650:7197-7216 None:intergenic 50.0%
CTGGCAAGACTTCCGAGTTA+TGG + tig0009650:7310-7329 None:intergenic 50.0%
CCGTTACGGACAAAATCCCT+TGG - tig0009650:7423-7442 Msa1347300:three_prime_UTR 50.0%
ACGGACAAAATCCCTTGGGA+CGG - tig0009650:7428-7447 Msa1347300:three_prime_UTR 50.0%
AAGAGATAGTCCCGTCCCAA+GGG + tig0009650:7442-7461 None:intergenic 50.0%
AAAGAGATAGTCCCGTCCCA+AGG + tig0009650:7443-7462 None:intergenic 50.0%
CATAGCCATGGAGGAAAGGA+AGG + tig0009650:7521-7540 None:intergenic 50.0%
CTTCCTTTCCTCCATGGCTA+TGG - tig0009650:7519-7538 Msa1347300:three_prime_UTR 50.0%
CTAAGCATGGAACAAGGCAG+TGG + tig0009650:7610-7629 None:intergenic 50.0%
TGCATGTCTCTCCCTATGTC+AGG - tig0009650:7655-7674 Msa1347300:CDS 50.0%
!! CTCTCCCTATGTCAGGTTGA+AGG - tig0009650:7662-7681 Msa1347300:CDS 50.0%
! ACAGAAGTGACTGGCCAATG+TGG - tig0009650:9022-9041 Msa1347300:intron 50.0%
TCGGTTGTAGGGCAAGTTGT+TGG - tig0009650:9665-9684 Msa1347300:intron 50.0%
! GATGTGATGGAGGAAGGAAG+GGG - tig0009650:9791-9810 Msa1347300:CDS 50.0%
CGGACAAAATCCCTTGGGAC+GGG - tig0009650:7429-7448 Msa1347300:three_prime_UTR 55.0%
GGTCCATAGCCATGGAGGAA+AGG + tig0009650:7525-7544 None:intergenic 55.0%
ATAGGGGTCCATAGCCATGG+AGG + tig0009650:7530-7549 None:intergenic 55.0%
GGAATAGGGGTCCATAGCCA+TGG + tig0009650:7533-7552 None:intergenic 55.0%
ATCCTTGTGGGACGAGCGAA+TGG - tig0009650:9570-9589 Msa1347300:CDS 55.0%
ATCCATTCGCTCGTCCCACA+AGG + tig0009650:9575-9594 None:intergenic 55.0%
ACGTTGTCCAGCATTGCCAG+AGG + tig0009650:9701-9720 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0009650 gene 7188 10105 7188 ID=Msa1347300;Name=Msa1347300
tig0009650 mRNA 7188 10105 7188 ID=Msa1347300-mRNA-1;Parent=Msa1347300;Name=Msa1347300-mRNA-1;_AED=0.13;_eAED=0.13;_QI=76|1|1|1|0.71|0.5|8|335|235
tig0009650 exon 7188 7270 7188 ID=Msa1347300-mRNA-1:exon:5863;Parent=Msa1347300-mRNA-1
tig0009650 exon 7302 7760 7302 ID=Msa1347300-mRNA-1:exon:5862;Parent=Msa1347300-mRNA-1
tig0009650 exon 8597 8694 8597 ID=Msa1347300-mRNA-1:exon:5861;Parent=Msa1347300-mRNA-1
tig0009650 exon 9036 9128 9036 ID=Msa1347300-mRNA-1:exon:5860;Parent=Msa1347300-mRNA-1
tig0009650 exon 9254 9341 9254 ID=Msa1347300-mRNA-1:exon:5859;Parent=Msa1347300-mRNA-1
tig0009650 exon 9566 9616 9566 ID=Msa1347300-mRNA-1:exon:5858;Parent=Msa1347300-mRNA-1
tig0009650 exon 9716 9890 9716 ID=Msa1347300-mRNA-1:exon:5857;Parent=Msa1347300-mRNA-1
tig0009650 exon 10034 10105 10034 ID=Msa1347300-mRNA-1:exon:5856;Parent=Msa1347300-mRNA-1
tig0009650 five_prime_UTR 10034 10105 10034 ID=Msa1347300-mRNA-1:five_prime_utr;Parent=Msa1347300-mRNA-1
tig0009650 five_prime_UTR 9887 9890 9887 ID=Msa1347300-mRNA-1:five_prime_utr;Parent=Msa1347300-mRNA-1
tig0009650 CDS 9716 9886 9716 ID=Msa1347300-mRNA-1:cds;Parent=Msa1347300-mRNA-1
tig0009650 CDS 9566 9616 9566 ID=Msa1347300-mRNA-1:cds;Parent=Msa1347300-mRNA-1
tig0009650 CDS 9254 9341 9254 ID=Msa1347300-mRNA-1:cds;Parent=Msa1347300-mRNA-1
tig0009650 CDS 9036 9128 9036 ID=Msa1347300-mRNA-1:cds;Parent=Msa1347300-mRNA-1
tig0009650 CDS 8597 8694 8597 ID=Msa1347300-mRNA-1:cds;Parent=Msa1347300-mRNA-1
tig0009650 CDS 7554 7760 7554 ID=Msa1347300-mRNA-1:cds;Parent=Msa1347300-mRNA-1
tig0009650 three_prime_UTR 7302 7553 7302 ID=Msa1347300-mRNA-1:three_prime_utr;Parent=Msa1347300-mRNA-1
tig0009650 three_prime_UTR 7188 7270 7188 ID=Msa1347300-mRNA-1:three_prime_utr;Parent=Msa1347300-mRNA-1
Gene Sequence

>Msa1347300

ATGGATTTCAAAGAAGCCGTTACGGACAAAATCCCTTGGGACGGGACTATCTCTTTAAATGATTTTGCCCTCTCTGCGTCTACATTTTCTGACAGGTGGAAAATGTTCAATCCTTCCTTTCCTCCATGGCTATGGACCCCTATTCCAAAACATCATTTGGGTACTCCTAAGGTTGAAGGATACTTATCACTTGAGAATGTGTGTCTTGTCAAATCAAGCGAGGAAGAAGAGAGTAACATAAGTCTGACATTGAATGCAGAGAGCATCATCTCACGAACAGAAGAGCCCTTTGATGATGCAACTTTAGTTTGTCCAGAGAATGAAGTAAATCACTATGATTTTCACATTGTCTACAGTCCTTCATATAGAGTTCCAGTGTTGTACTTCCGTTCGTACCATAGTGATGGACAACCTTTGCCAATTAGTGAAATTGAAAAGGACCTTCCTGGTCATTCTGCAGAATTGCTATTAGAATCCAAATGGACATTTATAACTCAAGATGAACACCCATATTTGAACAGGCCATGGTACAAGTTACATCCTTGTGGGACGAGCGAATGGATGAAACTGCTCTTCCATGGTGATACATCTTTGAATAGAAACTTCATTGAACAATATCTAGTTTCTTGGTTCTCGGTTGTAGGGCAAGTTGTTGGCCTTAAAACTCCTCTGGCAATGCTGGACAACGTAGTTTCAAATGATTCTTAG
Protein sequence

>Msa1347300

MDFKEAVTDKIPWDGTISLNDFALSASTFSDRWKMFNPSFPPWLWTPIPKHHLGTPKVEGYLSLENVCLVKSSEEEESNISLTLNAESIISRTEEPFDDATLVCPENEVNHYDFHIVYSPSYRVPVLYFRSYHSDGQPLPISEIEKDLPGHSAELLLESKWTFITQDEHPYLNRPWYKLHPCGTSEWMKLLFHGDTSLNRNFIEQYLVSWFSVVGQVVGLKTPLAMLDNVVSNDS*