Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1350970 | AES72155.1 | 90.741 | 108 | 4 | 1 | 1 | 108 | 1 | 102 | 9.72e-66 | 203 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1350970 | G7J362 | 90.741 | 108 | 4 | 1 | 1 | 108 | 1 | 102 | 4.64e-66 | 203 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1350970 | Msa1357600 | 0.923435 | 2.666539e-89 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1350970 | MtrunA17_Chr3g0123591 | 90.741 | 108 | 4 | 1 | 1 | 108 | 1 | 102 | 8.94e-70 | 203 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCTTCTACTGGTGTTAT+AGG | 0.161192 | tig0011997:+12399 | None:intergenic |
| ATCCACGATATTTGATGTTT+TGG | 0.242502 | tig0011997:+12547 | None:intergenic |
| ATTCTCTAGACTCAAGTCTT+TGG | 0.289644 | tig0011997:-12262 | Msa1350970:CDS |
| TTCTCTAGACTCAAGTCTTT+GGG | 0.349538 | tig0011997:-12261 | Msa1350970:CDS |
| AGCTGATGCGTTTATTAAGA+TGG | 0.412385 | tig0011997:-12301 | Msa1350970:CDS |
| GCATAGGCTCTGTTACTTCA+TGG | 0.428885 | tig0011997:+12493 | None:intergenic |
| TTCATGGACTTGGTAATTGT+AGG | 0.456574 | tig0011997:+12509 | None:intergenic |
| GACTTGGTAATTGTAGGAGT+TGG | 0.503865 | tig0011997:+12515 | None:intergenic |
| GCTCTGTTACTTCATGGACT+TGG | 0.511932 | tig0011997:+12499 | None:intergenic |
| TCATAGCGTTTGCGAAGCAT+AGG | 0.523104 | tig0011997:+12477 | None:intergenic |
| TTATTAAGATGGAGCATAGG+AGG | 0.556034 | tig0011997:-12290 | Msa1350970:CDS |
| TGGTAATTGTAGGAGTTGGA+AGG | 0.556659 | tig0011997:+12519 | None:intergenic |
| ACATCAGTCTCAGCCACAGC+TGG | 0.561442 | tig0011997:+12369 | None:intergenic |
| TCGTCCACGGAATCATAAGA+AGG | 0.564953 | tig0011997:-12454 | Msa1350970:CDS |
| TGAATTTACACGTTCAGTAG+TGG | 0.575967 | tig0011997:+12344 | None:intergenic |
| CGCATTCAAAACTGTGCTAG+AGG | 0.576519 | tig0011997:-12424 | Msa1350970:CDS |
| AACCAAAACATCAAATATCG+TGG | 0.581020 | tig0011997:-12549 | Msa1350970:CDS |
| AAAACATCAAATATCGTGGA+TGG | 0.597246 | tig0011997:-12545 | Msa1350970:CDS |
| TGACCCTTCTTATGATTCCG+TGG | 0.597578 | tig0011997:+12450 | None:intergenic |
| CGTTTATTAAGATGGAGCAT+AGG | 0.600428 | tig0011997:-12293 | Msa1350970:CDS |
| GGTAATTGTAGGAGTTGGAA+GGG | 0.608236 | tig0011997:+12520 | None:intergenic |
| AGAAGAAGAAGAACCAGCTG+TGG | 0.611828 | tig0011997:-12382 | Msa1350970:CDS |
| CGTCCACGGAATCATAAGAA+GGG | 0.624606 | tig0011997:-12453 | Msa1350970:CDS |
| GCAAACGCTATGATCGTCCA+CGG | 0.668943 | tig0011997:-12467 | Msa1350970:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACCAAAACATCAAATATCG+TGG | - | tig0011997:12257-12276 | Msa1350970:CDS | 30.0% | |
| ! | ATCCACGATATTTGATGTTT+TGG | + | tig0011997:12262-12281 | None:intergenic | 30.0% |
| AAAACATCAAATATCGTGGA+TGG | - | tig0011997:12261-12280 | Msa1350970:CDS | 30.0% | |
| ! | TTCATGGACTTGGTAATTGT+AGG | + | tig0011997:12300-12319 | None:intergenic | 35.0% |
| !! | TCTTCTTCTACTGGTGTTAT+AGG | + | tig0011997:12410-12429 | None:intergenic | 35.0% |
| TGAATTTACACGTTCAGTAG+TGG | + | tig0011997:12465-12484 | None:intergenic | 35.0% | |
| AGCTGATGCGTTTATTAAGA+TGG | - | tig0011997:12505-12524 | Msa1350970:CDS | 35.0% | |
| CGTTTATTAAGATGGAGCAT+AGG | - | tig0011997:12513-12532 | Msa1350970:CDS | 35.0% | |
| TTATTAAGATGGAGCATAGG+AGG | - | tig0011997:12516-12535 | Msa1350970:CDS | 35.0% | |
| ATTCTCTAGACTCAAGTCTT+TGG | - | tig0011997:12544-12563 | Msa1350970:CDS | 35.0% | |
| TTCTCTAGACTCAAGTCTTT+GGG | - | tig0011997:12545-12564 | Msa1350970:CDS | 35.0% | |
| GGTAATTGTAGGAGTTGGAA+GGG | + | tig0011997:12289-12308 | None:intergenic | 40.0% | |
| TGGTAATTGTAGGAGTTGGA+AGG | + | tig0011997:12290-12309 | None:intergenic | 40.0% | |
| ! | GACTTGGTAATTGTAGGAGT+TGG | + | tig0011997:12294-12313 | None:intergenic | 40.0% |
| GGTTCTTCTTCTTCTTCTAC+TGG | + | tig0011997:12419-12438 | None:intergenic | 40.0% | |
| ! | GCTCTGTTACTTCATGGACT+TGG | + | tig0011997:12310-12329 | None:intergenic | 45.0% |
| ! | GCATAGGCTCTGTTACTTCA+TGG | + | tig0011997:12316-12335 | None:intergenic | 45.0% |
| TCATAGCGTTTGCGAAGCAT+AGG | + | tig0011997:12332-12351 | None:intergenic | 45.0% | |
| TCGTCCACGGAATCATAAGA+AGG | - | tig0011997:12352-12371 | Msa1350970:CDS | 45.0% | |
| CGTCCACGGAATCATAAGAA+GGG | - | tig0011997:12353-12372 | Msa1350970:CDS | 45.0% | |
| TGACCCTTCTTATGATTCCG+TGG | + | tig0011997:12359-12378 | None:intergenic | 45.0% | |
| CGCATTCAAAACTGTGCTAG+AGG | - | tig0011997:12382-12401 | Msa1350970:CDS | 45.0% | |
| AGAAGAAGAAGAACCAGCTG+TGG | - | tig0011997:12424-12443 | Msa1350970:CDS | 45.0% | |
| GCAAACGCTATGATCGTCCA+CGG | - | tig0011997:12339-12358 | Msa1350970:CDS | 50.0% | |
| ACATCAGTCTCAGCCACAGC+TGG | + | tig0011997:12440-12459 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0011997 | gene | 12251 | 12577 | 12251 | ID=Msa1350970;Name=Msa1350970 |
| tig0011997 | mRNA | 12251 | 12577 | 12251 | ID=Msa1350970-mRNA-1;Parent=Msa1350970;Name=Msa1350970-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|108 |
| tig0011997 | exon | 12251 | 12577 | 12251 | ID=Msa1350970-mRNA-1:exon:7609;Parent=Msa1350970-mRNA-1 |
| tig0011997 | CDS | 12251 | 12577 | 12251 | ID=Msa1350970-mRNA-1:cds;Parent=Msa1350970-mRNA-1 |
| Gene Sequence |
| Protein sequence |
