Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1357600 | AES72155.1 | 90.741 | 108 | 4 | 1 | 1 | 108 | 1 | 102 | 9.72e-66 | 203 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1357600 | G7J362 | 90.741 | 108 | 4 | 1 | 1 | 108 | 1 | 102 | 4.64e-66 | 203 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1350970 | Msa1357600 | 0.923435 | 2.666539e-89 | -8.615850e-47 |
| Msa1357600 | Msa1445250 | 0.802039 | 6.777834e-49 | -8.615850e-47 |
| Msa0641020 | Msa1357600 | 0.801987 | 6.945803e-49 | -8.615850e-47 |
| Msa1244830 | Msa1357600 | 0.855647 | 5.481172e-62 | -8.615850e-47 |
| Msa1245210 | Msa1357600 | 0.818231 | 2.183359e-52 | -8.615850e-47 |
| Msa0457370 | Msa1357600 | 0.822779 | 1.973914e-53 | -8.615850e-47 |
| Msa1135660 | Msa1357600 | 0.804781 | 1.831933e-49 | -8.615850e-47 |
| Msa1166370 | Msa1357600 | 0.802362 | 5.816632e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1357600 | MtrunA17_Chr3g0123591 | 90.741 | 108 | 4 | 1 | 1 | 108 | 1 | 102 | 8.94e-70 | 203 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCTTCTACTGGTGTTAT+AGG | 0.161192 | tig0016100:-10783 | None:intergenic |
| ATCCACGATATTTGATGTTT+TGG | 0.242502 | tig0016100:-10635 | None:intergenic |
| ATTCTCTAGACTCAAGTCTT+TGG | 0.289644 | tig0016100:+10920 | Msa1357600:CDS |
| TTCTCTAGACTCAAGTCTTT+GGG | 0.349538 | tig0016100:+10921 | Msa1357600:CDS |
| AGCTGATGCGTTTATTAAGA+TGG | 0.412385 | tig0016100:+10881 | Msa1357600:CDS |
| GCATAGGCTCTGTTACTTCA+TGG | 0.428885 | tig0016100:-10689 | None:intergenic |
| TTCATGGACTTGGTAATTGT+AGG | 0.456574 | tig0016100:-10673 | None:intergenic |
| GACTTGGTAATTGTAGGAGT+TGG | 0.503865 | tig0016100:-10667 | None:intergenic |
| GCTCTGTTACTTCATGGACT+TGG | 0.511932 | tig0016100:-10683 | None:intergenic |
| TCATAGCGTTTGCGAAGCAT+AGG | 0.523104 | tig0016100:-10705 | None:intergenic |
| TTATTAAGATGGAGCATAGG+AGG | 0.556034 | tig0016100:+10892 | Msa1357600:CDS |
| TGGTAATTGTAGGAGTTGGA+AGG | 0.556659 | tig0016100:-10663 | None:intergenic |
| ACATCAGTCTCAGCCACAGC+TGG | 0.561442 | tig0016100:-10813 | None:intergenic |
| TCGTCCACGGAATCATAAGA+AGG | 0.564953 | tig0016100:+10728 | Msa1357600:CDS |
| TGAATTTACACGTTCAGTAG+TGG | 0.575967 | tig0016100:-10838 | None:intergenic |
| CGCATTCAAAACTGTGCTAG+AGG | 0.576519 | tig0016100:+10758 | Msa1357600:CDS |
| AACCAAAACATCAAATATCG+TGG | 0.581020 | tig0016100:+10633 | Msa1357600:CDS |
| AAAACATCAAATATCGTGGA+TGG | 0.597246 | tig0016100:+10637 | Msa1357600:CDS |
| TGACCCTTCTTATGATTCCG+TGG | 0.597578 | tig0016100:-10732 | None:intergenic |
| CGTTTATTAAGATGGAGCAT+AGG | 0.600428 | tig0016100:+10889 | Msa1357600:CDS |
| GGTAATTGTAGGAGTTGGAA+GGG | 0.608236 | tig0016100:-10662 | None:intergenic |
| AGAAGAAGAAGAACCAGCTG+TGG | 0.611828 | tig0016100:+10800 | Msa1357600:CDS |
| CGTCCACGGAATCATAAGAA+GGG | 0.624606 | tig0016100:+10729 | Msa1357600:CDS |
| GCAAACGCTATGATCGTCCA+CGG | 0.668943 | tig0016100:+10715 | Msa1357600:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACCAAAACATCAAATATCG+TGG | + | tig0016100:10633-10652 | Msa1357600:CDS | 30.0% | |
| ! | ATCCACGATATTTGATGTTT+TGG | - | tig0016100:10638-10657 | None:intergenic | 30.0% |
| AAAACATCAAATATCGTGGA+TGG | + | tig0016100:10637-10656 | Msa1357600:CDS | 30.0% | |
| ! | TTCATGGACTTGGTAATTGT+AGG | - | tig0016100:10676-10695 | None:intergenic | 35.0% |
| !! | TCTTCTTCTACTGGTGTTAT+AGG | - | tig0016100:10786-10805 | None:intergenic | 35.0% |
| TGAATTTACACGTTCAGTAG+TGG | - | tig0016100:10841-10860 | None:intergenic | 35.0% | |
| AGCTGATGCGTTTATTAAGA+TGG | + | tig0016100:10881-10900 | Msa1357600:CDS | 35.0% | |
| CGTTTATTAAGATGGAGCAT+AGG | + | tig0016100:10889-10908 | Msa1357600:CDS | 35.0% | |
| TTATTAAGATGGAGCATAGG+AGG | + | tig0016100:10892-10911 | Msa1357600:CDS | 35.0% | |
| ATTCTCTAGACTCAAGTCTT+TGG | + | tig0016100:10920-10939 | Msa1357600:CDS | 35.0% | |
| TTCTCTAGACTCAAGTCTTT+GGG | + | tig0016100:10921-10940 | Msa1357600:CDS | 35.0% | |
| GGTAATTGTAGGAGTTGGAA+GGG | - | tig0016100:10665-10684 | None:intergenic | 40.0% | |
| TGGTAATTGTAGGAGTTGGA+AGG | - | tig0016100:10666-10685 | None:intergenic | 40.0% | |
| ! | GACTTGGTAATTGTAGGAGT+TGG | - | tig0016100:10670-10689 | None:intergenic | 40.0% |
| GGTTCTTCTTCTTCTTCTAC+TGG | - | tig0016100:10795-10814 | None:intergenic | 40.0% | |
| ! | GCTCTGTTACTTCATGGACT+TGG | - | tig0016100:10686-10705 | None:intergenic | 45.0% |
| ! | GCATAGGCTCTGTTACTTCA+TGG | - | tig0016100:10692-10711 | None:intergenic | 45.0% |
| TCATAGCGTTTGCGAAGCAT+AGG | - | tig0016100:10708-10727 | None:intergenic | 45.0% | |
| TCGTCCACGGAATCATAAGA+AGG | + | tig0016100:10728-10747 | Msa1357600:CDS | 45.0% | |
| CGTCCACGGAATCATAAGAA+GGG | + | tig0016100:10729-10748 | Msa1357600:CDS | 45.0% | |
| TGACCCTTCTTATGATTCCG+TGG | - | tig0016100:10735-10754 | None:intergenic | 45.0% | |
| CGCATTCAAAACTGTGCTAG+AGG | + | tig0016100:10758-10777 | Msa1357600:CDS | 45.0% | |
| AGAAGAAGAAGAACCAGCTG+TGG | + | tig0016100:10800-10819 | Msa1357600:CDS | 45.0% | |
| GCAAACGCTATGATCGTCCA+CGG | + | tig0016100:10715-10734 | Msa1357600:CDS | 50.0% | |
| ACATCAGTCTCAGCCACAGC+TGG | - | tig0016100:10816-10835 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0016100 | gene | 10627 | 10953 | 10627 | ID=Msa1357600;Name=Msa1357600 |
| tig0016100 | mRNA | 10627 | 10953 | 10627 | ID=Msa1357600-mRNA-1;Parent=Msa1357600;Name=Msa1357600-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|108 |
| tig0016100 | exon | 10627 | 10953 | 10627 | ID=Msa1357600-mRNA-1:exon:10449;Parent=Msa1357600-mRNA-1 |
| tig0016100 | CDS | 10627 | 10953 | 10627 | ID=Msa1357600-mRNA-1:cds;Parent=Msa1357600-mRNA-1 |
| Gene Sequence |
| Protein sequence |
