Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1355990 | AET01855.1 | 91.818 | 110 | 7 | 1 | 1 | 108 | 1 | 110 | 4.64e-64 | 199 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1355990 | G7L8D4 | 91.818 | 110 | 7 | 1 | 1 | 108 | 1 | 110 | 2.22e-64 | 199 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1355990 | Msa1461500 | 0.967686 | 1.276255e-127 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1355990 | MtrunA17_Chr8g0344741 | 91.818 | 110 | 7 | 1 | 1 | 108 | 1 | 110 | 4.27e-68 | 199 |
| Msa1355990 | MtrunA17_Chr1g0156341 | 45.714 | 70 | 33 | 2 | 29 | 96 | 37 | 103 | 1.29e-12 | 60.1 |
| Msa1355990 | MtrunA17_Chr1g0147571 | 50.820 | 61 | 27 | 1 | 29 | 89 | 104 | 161 | 4.47e-12 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATTTGTTCCAACACATAT+TGG | 0.283512 | tig0015361:+2766 | Msa1355990:intergenic |
| CTGAGGAGGTATATGGATTT+AGG | 0.294014 | tig0015361:-2669 | Msa1355990:CDS |
| GGTAAGTCTGAGGAGGTATA+TGG | 0.356687 | tig0015361:-2676 | Msa1355990:CDS |
| ACTATCTCTAATAATCAAAT+TGG | 0.362113 | tig0015361:+2550 | Msa1355990:intergenic |
| TCAAACTCTTGTGCTTCAAA+TGG | 0.381185 | tig0015361:+2625 | Msa1355990:intergenic |
| ATTTGAAGCACAAGAGTTTG+AGG | 0.432023 | tig0015361:-2623 | Msa1355990:CDS |
| CAAACAAGATTAAAATTAAG+AGG | 0.446770 | tig0015361:-2582 | Msa1355990:CDS |
| CAAACTCTTGTGCTTCAAAT+GGG | 0.482219 | tig0015361:+2626 | Msa1355990:intergenic |
| GTATATGGATTTAGGAATGA+AGG | 0.484883 | tig0015361:-2661 | Msa1355990:CDS |
| TATATGGATTTAGGAATGAA+GGG | 0.508497 | tig0015361:-2660 | Msa1355990:CDS |
| AAGTTAAGGAAATTGATTAG+TGG | 0.514588 | tig0015361:-2850 | Msa1355990:CDS |
| ATGATTCACACAAGAGCACT+TGG | 0.514964 | tig0015361:-2724 | Msa1355990:CDS |
| TTAAGGAAATTGATTAGTGG+TGG | 0.530942 | tig0015361:-2847 | Msa1355990:CDS |
| CATATTGGAACATAACCTCT+TGG | 0.534719 | tig0015361:+2781 | Msa1355990:intergenic |
| GAGTTTGAGGAGTGGTTCAT+CGG | 0.540759 | tig0015361:-2610 | Msa1355990:CDS |
| CAAATGAAGAGACAAGTAAA+CGG | 0.545145 | tig0015361:-2750 | Msa1355990:CDS |
| GAGCTGGAGAAAGCAAGTTA+AGG | 0.550880 | tig0015361:-2864 | Msa1355990:CDS |
| GGTTATGTTCCAATATGTGT+TGG | 0.550977 | tig0015361:-2775 | Msa1355990:CDS |
| CAAGATTAAAATTAAGAGGA+TGG | 0.559437 | tig0015361:-2578 | Msa1355990:CDS |
| TTGAGGAGTGGTTCATCGGA+AGG | 0.567095 | tig0015361:-2606 | Msa1355990:CDS |
| TATACAAGGTCCTATGTTGT+TGG | 0.580732 | tig0015361:-2806 | Msa1355990:CDS |
| GCTTACTACAAACGTATACA+AGG | 0.583041 | tig0015361:-2820 | Msa1355990:CDS |
| CCTCTTGGTACCAACAACAT+AGG | 0.583945 | tig0015361:+2796 | Msa1355990:intergenic |
| TGAATTGTTGGGTAAGTCTG+AGG | 0.598628 | tig0015361:-2686 | Msa1355990:CDS |
| ATTGTTGGGTAAGTCTGAGG+AGG | 0.604492 | tig0015361:-2683 | Msa1355990:CDS |
| ATATGGATTTAGGAATGAAG+GGG | 0.611045 | tig0015361:-2659 | Msa1355990:CDS |
| CCTATGTTGTTGGTACCAAG+AGG | 0.657542 | tig0015361:-2796 | Msa1355990:CDS |
| AAGCACAAGAGTTTGAGGAG+TGG | 0.660626 | tig0015361:-2618 | Msa1355990:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAAACAAGATTAAAATTAAG+AGG | - | tig0015361:2850-2869 | Msa1355990:CDS | 20.0% |
| ! | AAGTTAAGGAAATTGATTAG+TGG | - | tig0015361:2582-2601 | Msa1355990:CDS | 25.0% |
| ! | TATATGGATTTAGGAATGAA+GGG | - | tig0015361:2772-2791 | Msa1355990:CDS | 25.0% |
| ! | CAAGATTAAAATTAAGAGGA+TGG | - | tig0015361:2854-2873 | Msa1355990:CDS | 25.0% |
| TTAAGGAAATTGATTAGTGG+TGG | - | tig0015361:2585-2604 | Msa1355990:CDS | 30.0% | |
| TCATTTGTTCCAACACATAT+TGG | + | tig0015361:2669-2688 | Msa1355990:intergenic | 30.0% | |
| CAAATGAAGAGACAAGTAAA+CGG | - | tig0015361:2682-2701 | Msa1355990:CDS | 30.0% | |
| !!! | TGCATTTTTTCGTGAATTGT+TGG | - | tig0015361:2734-2753 | Msa1355990:CDS | 30.0% |
| !!! | GCATTTTTTCGTGAATTGTT+GGG | - | tig0015361:2735-2754 | Msa1355990:CDS | 30.0% |
| GTATATGGATTTAGGAATGA+AGG | - | tig0015361:2771-2790 | Msa1355990:CDS | 30.0% | |
| ATATGGATTTAGGAATGAAG+GGG | - | tig0015361:2773-2792 | Msa1355990:CDS | 30.0% | |
| GCTTACTACAAACGTATACA+AGG | - | tig0015361:2612-2631 | Msa1355990:CDS | 35.0% | |
| TATACAAGGTCCTATGTTGT+TGG | - | tig0015361:2626-2645 | Msa1355990:CDS | 35.0% | |
| CATATTGGAACATAACCTCT+TGG | + | tig0015361:2654-2673 | Msa1355990:intergenic | 35.0% | |
| GGTTATGTTCCAATATGTGT+TGG | - | tig0015361:2657-2676 | Msa1355990:CDS | 35.0% | |
| CAAACTCTTGTGCTTCAAAT+GGG | + | tig0015361:2809-2828 | Msa1355990:intergenic | 35.0% | |
| TCAAACTCTTGTGCTTCAAA+TGG | + | tig0015361:2810-2829 | Msa1355990:intergenic | 35.0% | |
| ! | ATTTGAAGCACAAGAGTTTG+AGG | - | tig0015361:2809-2828 | Msa1355990:CDS | 35.0% |
| !! | ATGATTCACACAAGAGCACT+TGG | - | tig0015361:2708-2727 | Msa1355990:CDS | 40.0% |
| TGAATTGTTGGGTAAGTCTG+AGG | - | tig0015361:2746-2765 | Msa1355990:CDS | 40.0% | |
| ! | CTGAGGAGGTATATGGATTT+AGG | - | tig0015361:2763-2782 | Msa1355990:CDS | 40.0% |
| ! | TTAGGAATGAAGGGGTTTTG+AGG | - | tig0015361:2781-2800 | Msa1355990:CDS | 40.0% |
| GAGCTGGAGAAAGCAAGTTA+AGG | - | tig0015361:2568-2587 | Msa1355990:CDS | 45.0% | |
| CCTCTTGGTACCAACAACAT+AGG | + | tig0015361:2639-2658 | Msa1355990:intergenic | 45.0% | |
| CCTATGTTGTTGGTACCAAG+AGG | - | tig0015361:2636-2655 | Msa1355990:CDS | 45.0% | |
| ATTGTTGGGTAAGTCTGAGG+AGG | - | tig0015361:2749-2768 | Msa1355990:CDS | 45.0% | |
| ! | GGTAAGTCTGAGGAGGTATA+TGG | - | tig0015361:2756-2775 | Msa1355990:CDS | 45.0% |
| AAGCACAAGAGTTTGAGGAG+TGG | - | tig0015361:2814-2833 | Msa1355990:CDS | 45.0% | |
| GAGTTTGAGGAGTGGTTCAT+CGG | - | tig0015361:2822-2841 | Msa1355990:CDS | 45.0% | |
| TTGAGGAGTGGTTCATCGGA+AGG | - | tig0015361:2826-2845 | Msa1355990:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0015361 | gene | 2564 | 2890 | 2564 | ID=Msa1355990;Name=Msa1355990 |
| tig0015361 | mRNA | 2564 | 2890 | 2564 | ID=Msa1355990-mRNA-1;Parent=Msa1355990;Name=Msa1355990-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|108 |
| tig0015361 | exon | 2564 | 2890 | 2564 | ID=Msa1355990-mRNA-1:exon:9683;Parent=Msa1355990-mRNA-1 |
| tig0015361 | CDS | 2564 | 2890 | 2564 | ID=Msa1355990-mRNA-1:cds;Parent=Msa1355990-mRNA-1 |
| Gene Sequence |
| Protein sequence |
