Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1461500 | AET01855.1 | 91.818 | 110 | 7 | 1 | 1 | 108 | 1 | 110 | 4.64e-64 | 199 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1461500 | G7L8D4 | 91.818 | 110 | 7 | 1 | 1 | 108 | 1 | 110 | 2.22e-64 | 199 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0101910 | Msa1461500 | 0.803484 | 3.409551e-49 | -8.615850e-47 |
| Msa1355990 | Msa1461500 | 0.967686 | 1.276255e-127 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1461500 | MtrunA17_Chr8g0344741 | 91.818 | 110 | 7 | 1 | 1 | 108 | 1 | 110 | 4.27e-68 | 199 |
| Msa1461500 | MtrunA17_Chr1g0156341 | 45.714 | 70 | 33 | 2 | 29 | 96 | 37 | 103 | 1.29e-12 | 60.1 |
| Msa1461500 | MtrunA17_Chr1g0147571 | 50.820 | 61 | 27 | 1 | 29 | 89 | 104 | 161 | 4.47e-12 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATTTGTTCCAACACATAT+TGG | 0.283512 | tig0041568:-10013 | None:intergenic |
| CTGAGGAGGTATATGGATTT+AGG | 0.294014 | tig0041568:+10110 | Msa1461500:CDS |
| GGTAAGTCTGAGGAGGTATA+TGG | 0.356687 | tig0041568:+10103 | Msa1461500:CDS |
| ACTATCTCTAATAATCAAAT+TGG | 0.362113 | tig0041568:-10229 | None:intergenic |
| TCAAACTCTTGTGCTTCAAA+TGG | 0.381185 | tig0041568:-10154 | None:intergenic |
| ATTTGAAGCACAAGAGTTTG+AGG | 0.432023 | tig0041568:+10156 | Msa1461500:CDS |
| CAAACAAGATTAAAATTAAG+AGG | 0.446770 | tig0041568:+10197 | Msa1461500:CDS |
| CAAACTCTTGTGCTTCAAAT+GGG | 0.482219 | tig0041568:-10153 | None:intergenic |
| GTATATGGATTTAGGAATGA+AGG | 0.484883 | tig0041568:+10118 | Msa1461500:CDS |
| TATATGGATTTAGGAATGAA+GGG | 0.508497 | tig0041568:+10119 | Msa1461500:CDS |
| AAGTTAAGGAAATTGATTAG+TGG | 0.514588 | tig0041568:+9929 | Msa1461500:CDS |
| ATGATTCACACAAGAGCACT+TGG | 0.514964 | tig0041568:+10055 | Msa1461500:CDS |
| TTAAGGAAATTGATTAGTGG+TGG | 0.530942 | tig0041568:+9932 | Msa1461500:CDS |
| CATATTGGAACATAACCTCT+TGG | 0.534719 | tig0041568:-9998 | None:intergenic |
| GAGTTTGAGGAGTGGTTCAT+CGG | 0.540759 | tig0041568:+10169 | Msa1461500:CDS |
| CAAATGAAGAGACAAGTAAA+CGG | 0.545145 | tig0041568:+10029 | Msa1461500:CDS |
| GAGCTGGAGAAAGCAAGTTA+AGG | 0.550880 | tig0041568:+9915 | Msa1461500:CDS |
| GGTTATGTTCCAATATGTGT+TGG | 0.550977 | tig0041568:+10004 | Msa1461500:CDS |
| CAAGATTAAAATTAAGAGGA+TGG | 0.559437 | tig0041568:+10201 | Msa1461500:CDS |
| TTGAGGAGTGGTTCATCGGA+AGG | 0.567095 | tig0041568:+10173 | Msa1461500:CDS |
| TATACAAGGTCCTATGTTGT+TGG | 0.580732 | tig0041568:+9973 | Msa1461500:CDS |
| GCTTACTACAAACGTATACA+AGG | 0.583041 | tig0041568:+9959 | Msa1461500:CDS |
| CCTCTTGGTACCAACAACAT+AGG | 0.583945 | tig0041568:-9983 | None:intergenic |
| TGAATTGTTGGGTAAGTCTG+AGG | 0.598628 | tig0041568:+10093 | Msa1461500:CDS |
| ATTGTTGGGTAAGTCTGAGG+AGG | 0.604492 | tig0041568:+10096 | Msa1461500:CDS |
| ATATGGATTTAGGAATGAAG+GGG | 0.611045 | tig0041568:+10120 | Msa1461500:CDS |
| CCTATGTTGTTGGTACCAAG+AGG | 0.657542 | tig0041568:+9983 | Msa1461500:CDS |
| AAGCACAAGAGTTTGAGGAG+TGG | 0.660626 | tig0041568:+10161 | Msa1461500:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAAACAAGATTAAAATTAAG+AGG | + | tig0041568:10197-10216 | Msa1461500:CDS | 20.0% |
| ! | AAGTTAAGGAAATTGATTAG+TGG | + | tig0041568:9929-9948 | Msa1461500:CDS | 25.0% |
| ! | TATATGGATTTAGGAATGAA+GGG | + | tig0041568:10119-10138 | Msa1461500:CDS | 25.0% |
| ! | CAAGATTAAAATTAAGAGGA+TGG | + | tig0041568:10201-10220 | Msa1461500:CDS | 25.0% |
| TTAAGGAAATTGATTAGTGG+TGG | + | tig0041568:9932-9951 | Msa1461500:CDS | 30.0% | |
| TCATTTGTTCCAACACATAT+TGG | - | tig0041568:10016-10035 | None:intergenic | 30.0% | |
| CAAATGAAGAGACAAGTAAA+CGG | + | tig0041568:10029-10048 | Msa1461500:CDS | 30.0% | |
| !!! | TGCATTTTTTCGTGAATTGT+TGG | + | tig0041568:10081-10100 | Msa1461500:CDS | 30.0% |
| !!! | GCATTTTTTCGTGAATTGTT+GGG | + | tig0041568:10082-10101 | Msa1461500:CDS | 30.0% |
| GTATATGGATTTAGGAATGA+AGG | + | tig0041568:10118-10137 | Msa1461500:CDS | 30.0% | |
| ATATGGATTTAGGAATGAAG+GGG | + | tig0041568:10120-10139 | Msa1461500:CDS | 30.0% | |
| GCTTACTACAAACGTATACA+AGG | + | tig0041568:9959-9978 | Msa1461500:CDS | 35.0% | |
| TATACAAGGTCCTATGTTGT+TGG | + | tig0041568:9973-9992 | Msa1461500:CDS | 35.0% | |
| CATATTGGAACATAACCTCT+TGG | - | tig0041568:10001-10020 | None:intergenic | 35.0% | |
| GGTTATGTTCCAATATGTGT+TGG | + | tig0041568:10004-10023 | Msa1461500:CDS | 35.0% | |
| CAAACTCTTGTGCTTCAAAT+GGG | - | tig0041568:10156-10175 | None:intergenic | 35.0% | |
| TCAAACTCTTGTGCTTCAAA+TGG | - | tig0041568:10157-10176 | None:intergenic | 35.0% | |
| ! | ATTTGAAGCACAAGAGTTTG+AGG | + | tig0041568:10156-10175 | Msa1461500:CDS | 35.0% |
| !! | ATGATTCACACAAGAGCACT+TGG | + | tig0041568:10055-10074 | Msa1461500:CDS | 40.0% |
| TGAATTGTTGGGTAAGTCTG+AGG | + | tig0041568:10093-10112 | Msa1461500:CDS | 40.0% | |
| ! | CTGAGGAGGTATATGGATTT+AGG | + | tig0041568:10110-10129 | Msa1461500:CDS | 40.0% |
| ! | TTAGGAATGAAGGGGTTTTG+AGG | + | tig0041568:10128-10147 | Msa1461500:CDS | 40.0% |
| GAGCTGGAGAAAGCAAGTTA+AGG | + | tig0041568:9915-9934 | Msa1461500:CDS | 45.0% | |
| CCTCTTGGTACCAACAACAT+AGG | - | tig0041568:9986-10005 | None:intergenic | 45.0% | |
| CCTATGTTGTTGGTACCAAG+AGG | + | tig0041568:9983-10002 | Msa1461500:CDS | 45.0% | |
| ATTGTTGGGTAAGTCTGAGG+AGG | + | tig0041568:10096-10115 | Msa1461500:CDS | 45.0% | |
| ! | GGTAAGTCTGAGGAGGTATA+TGG | + | tig0041568:10103-10122 | Msa1461500:CDS | 45.0% |
| AAGCACAAGAGTTTGAGGAG+TGG | + | tig0041568:10161-10180 | Msa1461500:CDS | 45.0% | |
| GAGTTTGAGGAGTGGTTCAT+CGG | + | tig0041568:10169-10188 | Msa1461500:CDS | 45.0% | |
| TTGAGGAGTGGTTCATCGGA+AGG | + | tig0041568:10173-10192 | Msa1461500:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0041568 | gene | 9911 | 10237 | 9911 | ID=Msa1461500;Name=Msa1461500 |
| tig0041568 | mRNA | 9911 | 10237 | 9911 | ID=Msa1461500-mRNA-1;Parent=Msa1461500;Name=Msa1461500-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|108 |
| tig0041568 | exon | 9911 | 10237 | 9911 | ID=Msa1461500-mRNA-1:exon:23212;Parent=Msa1461500-mRNA-1 |
| tig0041568 | CDS | 9911 | 10237 | 9911 | ID=Msa1461500-mRNA-1:cds;Parent=Msa1461500-mRNA-1 |
| Gene Sequence |
| Protein sequence |
