Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1357020 | RHN80902.1 | 96.703 | 91 | 3 | 0 | 1 | 91 | 1 | 91 | 3.99e-57 | 181 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1357020 | A0A396JTN5 | 96.703 | 91 | 3 | 0 | 1 | 91 | 1 | 91 | 1.91e-57 | 181 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0909140 | Msa1357020 | 0.822727 | 2.029061e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1357020 | MtrunA17_Chr1g0193261 | 96.703 | 91 | 3 | 0 | 1 | 91 | 1 | 91 | 3.67e-61 | 181 |
| Msa1357020 | MtrunA17_Chr1g0193231 | 95.604 | 91 | 4 | 0 | 1 | 91 | 1 | 91 | 2.71e-52 | 158 |
| Msa1357020 | MtrunA17_Chr1g0193301 | 94.565 | 92 | 4 | 1 | 1 | 91 | 1 | 92 | 2.16e-50 | 153 |
| Msa1357020 | MtrunA17_Chr1g0193291 | 82.418 | 91 | 14 | 2 | 1 | 91 | 1 | 89 | 5.87e-42 | 132 |
| Msa1357020 | MtrunA17_Chr7g0271321 | 59.091 | 88 | 30 | 2 | 1 | 88 | 1 | 82 | 2.08e-29 | 100 |
| Msa1357020 | MtrunA17_Chr1g0193271 | 50.575 | 87 | 36 | 4 | 3 | 89 | 2 | 81 | 2.26e-20 | 77.4 |
| Msa1357020 | MtrunA17_Chr1g0193251 | 47.727 | 88 | 37 | 4 | 2 | 89 | 3 | 81 | 6.99e-16 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCTAAATCATCATCATTC+AGG | 0.343954 | tig0015879:-73896 | Msa1357020:CDS |
| GTAGCTCTCATGTCAATAAC+AGG | 0.408831 | tig0015879:+73741 | None:intergenic |
| TGATGAAGACAGAGGCAGTT+GGG | 0.418711 | tig0015879:-73775 | Msa1357020:CDS |
| CTGCCTCTGTCTTCATCACT+TGG | 0.423392 | tig0015879:+73780 | None:intergenic |
| TTTCTGAATCTGAAATTCAC+TGG | 0.475847 | tig0015879:-73689 | Msa1357020:CDS |
| GTTCCTATATCCTACTGATC+AGG | 0.494135 | tig0015879:+73657 | None:intergenic |
| AACAAGCAAAGAGGAGGAGG+AGG | 0.495003 | tig0015879:-73843 | Msa1357020:CDS |
| AGTAACAAGCAAAGAGGAGG+AGG | 0.512782 | tig0015879:-73846 | Msa1357020:CDS |
| GCAAGCTCAACCTGATCAGT+AGG | 0.568773 | tig0015879:-73667 | None:intergenic |
| GTGATGAAGACAGAGGCAGT+TGG | 0.578210 | tig0015879:-73776 | Msa1357020:CDS |
| GATGAAGACAGAGGCAGTTG+GGG | 0.597931 | tig0015879:-73774 | Msa1357020:CDS |
| TCTAGCAGTAACAAGCAAAG+AGG | 0.600955 | tig0015879:-73852 | Msa1357020:CDS |
| ACAAGCAAAGAGGAGGAGGA+GGG | 0.615182 | tig0015879:-73842 | Msa1357020:CDS |
| AAATGGGAGGAAACAGTCAA+AGG | 0.618697 | tig0015879:-73923 | Msa1357020:CDS |
| CTTCATATGATAACAAAGTG+TGG | 0.623723 | tig0015879:-73803 | Msa1357020:CDS |
| AGCAGTAACAAGCAAAGAGG+AGG | 0.630560 | tig0015879:-73849 | Msa1357020:CDS |
| TACCTCAGTAGTAAAATGGG+AGG | 0.663627 | tig0015879:-73936 | None:intergenic |
| TGGCCAAGTGATGAAGACAG+AGG | 0.693322 | tig0015879:-73783 | Msa1357020:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CATCTAAATCATCATCATTC+AGG | - | tig0015879:73693-73712 | Msa1357020:CDS | 30.0% | |
| CTTCATATGATAACAAAGTG+TGG | - | tig0015879:73786-73805 | Msa1357020:CDS | 30.0% | |
| ! | TTTCTGAATCTGAAATTCAC+TGG | - | tig0015879:73900-73919 | Msa1357020:CDS | 30.0% |
| TCTAGCAGTAACAAGCAAAG+AGG | - | tig0015879:73737-73756 | Msa1357020:CDS | 40.0% | |
| GTAGCTCTCATGTCAATAAC+AGG | + | tig0015879:73851-73870 | None:intergenic | 40.0% | |
| AGCAGTAACAAGCAAAGAGG+AGG | - | tig0015879:73740-73759 | Msa1357020:CDS | 45.0% | |
| AGTAACAAGCAAAGAGGAGG+AGG | - | tig0015879:73743-73762 | Msa1357020:CDS | 45.0% | |
| TGATGAAGACAGAGGCAGTT+GGG | - | tig0015879:73814-73833 | Msa1357020:CDS | 45.0% | |
| AACAAGCAAAGAGGAGGAGG+AGG | - | tig0015879:73746-73765 | Msa1357020:CDS | 50.0% | |
| ACAAGCAAAGAGGAGGAGGA+GGG | - | tig0015879:73747-73766 | Msa1357020:CDS | 50.0% | |
| TGGCCAAGTGATGAAGACAG+AGG | - | tig0015879:73806-73825 | Msa1357020:CDS | 50.0% | |
| CTGCCTCTGTCTTCATCACT+TGG | + | tig0015879:73812-73831 | None:intergenic | 50.0% | |
| GTGATGAAGACAGAGGCAGT+TGG | - | tig0015879:73813-73832 | Msa1357020:CDS | 50.0% | |
| GATGAAGACAGAGGCAGTTG+GGG | - | tig0015879:73815-73834 | Msa1357020:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0015879 | gene | 73668 | 73943 | 73668 | ID=Msa1357020;Name=Msa1357020 |
| tig0015879 | mRNA | 73668 | 73943 | 73668 | ID=Msa1357020-mRNA-1;Parent=Msa1357020;Name=Msa1357020-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|91 |
| tig0015879 | exon | 73668 | 73943 | 73668 | ID=Msa1357020-mRNA-1:exon:10225;Parent=Msa1357020-mRNA-1 |
| tig0015879 | CDS | 73668 | 73943 | 73668 | ID=Msa1357020-mRNA-1:cds;Parent=Msa1357020-mRNA-1 |
| Gene Sequence |
| Protein sequence |
