AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1365750


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1365750 MtrunA17_Chr3g0145061 95.876 194 8 0 1 194 1 194 3.63e-139 387
Msa1365750 MtrunA17_Chr4g0035651 60.309 194 68 4 1 193 1 186 5.13e-72 217
Msa1365750 MtrunA17_Chr3g0142221 52.151 186 45 3 1 185 1 143 7.42e-60 186
Msa1365750 MtrunA17_Chr1g0155811 50.515 194 85 3 1 194 1 183 3.03e-55 176
Msa1365750 MtrunA17_Chr2g0314671 55.738 122 53 1 1 122 1 121 2.11e-40 139
Msa1365750 MtrunA17_Chr4g0033641 45.390 141 69 4 1 140 1 134 3.23e-35 124
Msa1365750 MtrunA17_Chr2g0329511 52.294 109 51 1 1 109 1 108 8.85e-34 120
Msa1365750 MtrunA17_Chr5g0405151 36.429 140 79 3 1 134 1 136 5.99e-24 93.2
Msa1365750 MtrunA17_Chr1g0150471 39.048 105 54 1 5 109 6 100 8.34e-18 79.7
Msa1365750 MtrunA17_Chr3g0136701 37.113 97 51 1 5 101 6 92 3.66e-16 74.7
Msa1365750 MtrunA17_Chr4g0072871 39.785 93 46 1 5 97 22 104 5.70e-16 75.1
Msa1365750 MtrunA17_Chr5g0429341 30.769 195 103 8 1 176 1 182 8.82e-14 68.9
Msa1365750 MtrunA17_Chr7g0220721 36.559 93 49 1 5 97 26 108 1.20e-13 68.6
Msa1365750 MtrunA17_Chr3g0120711 34.021 97 53 2 1 97 1 86 9.18e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1365750 AT1G75540.1 51.456 206 88 4 1 194 1 206 2.63e-61 194
Msa1365750 AT4G39070.1 55.000 200 74 5 1 184 1 200 1.80e-56 179
Msa1365750 AT1G06040.2 52.206 136 62 2 1 136 1 133 1.37e-40 136
Msa1365750 AT1G78600.1 54.237 118 53 1 1 118 1 117 1.60e-39 137
Msa1365750 AT1G78600.2 54.237 118 53 1 1 118 1 117 2.54e-39 137
Msa1365750 AT1G06040.1 52.206 136 62 2 1 136 1 133 3.00e-39 135
Msa1365750 AT4G10240.1 51.799 139 57 3 1 133 1 135 1.07e-38 130
Msa1365750 AT2G31380.1 48.092 131 67 1 1 131 1 130 5.07e-36 126
Msa1365750 AT4G38960.1 39.316 117 67 2 1 117 1 113 1.39e-23 92.8
Msa1365750 AT4G38960.6 39.316 117 67 2 1 117 1 113 1.39e-23 92.8
Msa1365750 AT2G21320.1 44.000 100 52 2 1 100 1 96 1.16e-22 90.1
Msa1365750 AT2G24790.2 39.785 93 46 1 5 97 8 90 3.17e-17 77.0
Msa1365750 AT2G24790.1 38.144 97 50 1 5 101 8 94 9.66e-17 76.6
Msa1365750 AT5G24930.1 36.082 97 52 1 5 101 6 92 6.20e-16 75.1
Msa1365750 AT4G38960.3 28.750 160 67 3 1 117 1 156 1.37e-15 72.8
Msa1365750 AT5G15850.1 32.787 122 71 2 5 125 12 123 2.22e-14 70.9
Msa1365750 AT3G02380.1 34.286 105 57 2 5 109 16 108 1.56e-13 68.2
Msa1365750 AT2G47890.2 38.298 94 48 2 5 98 13 96 7.60e-13 65.5
Msa1365750 AT2G47890.3 41.176 85 40 2 5 89 13 87 1.33e-12 65.5
Msa1365750 AT2G47890.1 41.176 85 40 2 5 89 13 87 1.78e-12 65.1
Msa1365750 AT1G28050.1 34.653 101 56 1 2 102 6 96 2.68e-12 65.1
Msa1365750 AT5G15840.2 31.132 106 61 2 5 110 20 113 3.42e-12 63.9
Msa1365750 AT5G15840.1 31.132 106 61 2 5 110 20 113 3.99e-12 64.3
Msa1365750 AT2G33500.2 33.000 100 57 1 3 102 10 99 5.31e-12 63.9
Msa1365750 AT2G33500.1 33.000 100 57 1 3 102 10 99 5.47e-12 63.9

Find 38 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCTACTAGAGTAGTTTCT+TGG 0.265361 tig0018386:-49234 None:intergenic
GAAGCTTTCAAGCAATTCTT+TGG 0.294858 tig0018386:+49176 Msa1365750:CDS
GATGCTTCTTTCCCACCTAA+TGG 0.309132 tig0018386:+49417 Msa1365750:CDS
ATAGAATCGCTCTATCTTCT+TGG 0.325288 tig0018386:-49077 None:intergenic
CCCACCTAATGGTTTCTGTA+AGG 0.391077 tig0018386:+49428 Msa1365750:CDS
TAAACCTTACAGAAACCATT+AGG 0.399460 tig0018386:-49432 None:intergenic
TCTTGGTTCAGAGCCAATAC+TGG 0.403841 tig0018386:-49217 None:intergenic
ATCACAAAGAGGGTAGTCTT+TGG 0.421009 tig0018386:-48842 None:intergenic
AACATACACAGAAACATAAC+AGG 0.440782 tig0018386:+49136 Msa1365750:CDS
CATAACAGGTTTCTTCTCTC+TGG 0.447881 tig0018386:+49150 Msa1365750:CDS
GTGTATGTTCATTTGCTCCA+TGG 0.455691 tig0018386:-49122 None:intergenic
GTGTTTGGTTGCAAGCTTGT+TGG 0.456215 tig0018386:-48791 None:intergenic
CATTTGCTCCATGGATTGGA+AGG 0.479724 tig0018386:-49113 None:intergenic
ATACTGGTGGATGATGAATC+TGG 0.490828 tig0018386:-49201 None:intergenic
GATTCATCATCCACCAGTAT+TGG 0.497576 tig0018386:+49204 Msa1365750:CDS
TGTTCATTTGCTCCATGGAT+TGG 0.505507 tig0018386:-49117 None:intergenic
GAATGTGACCTTCCAATCCA+TGG 0.511555 tig0018386:+49105 Msa1365750:CDS
GAGGGTAGTCTTTGGAGTTG+AGG 0.523182 tig0018386:-48834 None:intergenic
GATGAAGCTGCTCTTTGCCA+TGG 0.528890 tig0018386:+48745 Msa1365750:CDS
GACAACATGGCTAGTGACAC+TGG 0.532967 tig0018386:+49321 Msa1365750:CDS
AAATGCAAGTTCTTCTTGCA+TGG 0.564285 tig0018386:+49293 Msa1365750:CDS
CTTTCTCACACACATCACAT+TGG 0.572968 tig0018386:-48696 None:intergenic
TGGTTCAGAGCCAATACTGG+TGG 0.580417 tig0018386:-49214 None:intergenic
TGGTTGCAAGCTTGTTGGCA+CGG 0.580653 tig0018386:-48786 None:intergenic
GGTAGTCTTTGGAGTTGAGG+TGG 0.585466 tig0018386:-48831 None:intergenic
TTCATCTGAAGGACAGAACA+TGG 0.588007 tig0018386:-48728 None:intergenic
TACTTGATTGAAACAATACC+TGG 0.589710 tig0018386:+49372 Msa1365750:CDS
ACCTTACAGAAACCATTAGG+TGG 0.591397 tig0018386:-49429 None:intergenic
CAAAGAGCAGCTTCATCTGA+AGG 0.591587 tig0018386:-48739 None:intergenic
GGATTGTATGATCACATCCA+TGG 0.608665 tig0018386:-48762 None:intergenic
TACTGGTGGATGATGAATCT+GGG 0.618443 tig0018386:-49200 None:intergenic
TTGCATGGCTGAAGACAACA+TGG 0.623591 tig0018386:+49308 Msa1365750:CDS
CCTTACAGAAACCATTAGGT+GGG 0.623605 tig0018386:-49428 None:intergenic
CTTGACAGATATCACAAAGA+GGG 0.634180 tig0018386:-48852 None:intergenic
AAGTCTTCAAAACAGTAACC+AGG 0.647020 tig0018386:-49390 None:intergenic
ACTTGACAGATATCACAAAG+AGG 0.665459 tig0018386:-48853 None:intergenic
TTGCAAGCTTGTTGGCACGG+TGG 0.712576 tig0018386:-48783 None:intergenic
TGATGTGTGTGAGAAAGCTG+AGG 0.739027 tig0018386:+48702 Msa1365750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CCGTTTTTATAAAAAAATGA+CGG - tig0018386:48888-48907 None:intergenic 20.0%
!!! CCGTCATTTTTTTATAAAAA+CGG + tig0018386:48885-48904 Msa1365750:intron 20.0%
!!! TTAATGACCATTTTAAAATG+TGG - tig0018386:48996-49015 None:intergenic 20.0%
!!! CCGTTTTTATAAAAAAATGA+CGG - tig0018386:48888-48907 None:intergenic 20.0%
!!! CCGTCATTTTTTTATAAAAA+CGG + tig0018386:48885-48904 Msa1365750:intron 20.0%
!!! TTAATGACCATTTTAAAATG+TGG - tig0018386:48996-49015 None:intergenic 20.0%
!!! TTTTTTTATAAAAACGGTCG+CGG + tig0018386:48891-48910 Msa1365750:intron 25.0%
!!! ACTCTAACCACATTTTAAAA+TGG + tig0018386:48986-49005 Msa1365750:intron 25.0%
!! TTTTCATTGGAAATTGATCT+TGG - tig0018386:49276-49295 None:intergenic 25.0%
!!! TTTTTTTATAAAAACGGTCG+CGG + tig0018386:48891-48910 Msa1365750:intron 25.0%
!!! ACTCTAACCACATTTTAAAA+TGG + tig0018386:48986-49005 Msa1365750:intron 25.0%
!! TTTTCATTGGAAATTGATCT+TGG - tig0018386:49276-49295 None:intergenic 25.0%
TTAAAATGTGGTTAGAGTTG+TGG - tig0018386:48984-49003 None:intergenic 30.0%
AACATACACAGAAACATAAC+AGG + tig0018386:49136-49155 Msa1365750:CDS 30.0%
! AGAAGAACTTGCATTTTCAT+TGG - tig0018386:49289-49308 None:intergenic 30.0%
TACTTGATTGAAACAATACC+TGG + tig0018386:49372-49391 Msa1365750:CDS 30.0%
TAAACCTTACAGAAACCATT+AGG - tig0018386:49435-49454 None:intergenic 30.0%
TTAAAATGTGGTTAGAGTTG+TGG - tig0018386:48984-49003 None:intergenic 30.0%
AACATACACAGAAACATAAC+AGG + tig0018386:49136-49155 Msa1365750:CDS 30.0%
! AGAAGAACTTGCATTTTCAT+TGG - tig0018386:49289-49308 None:intergenic 30.0%
TACTTGATTGAAACAATACC+TGG + tig0018386:49372-49391 Msa1365750:CDS 30.0%
TAAACCTTACAGAAACCATT+AGG - tig0018386:49435-49454 None:intergenic 30.0%
AAGAGAAAAACGAGTGTGTT+TGG - tig0018386:48809-48828 None:intergenic 35.0%
CTTGACAGATATCACAAAGA+GGG - tig0018386:48855-48874 None:intergenic 35.0%
ACTTGACAGATATCACAAAG+AGG - tig0018386:48856-48875 None:intergenic 35.0%
TATTAAAGATCCAGATGCGA+CGG - tig0018386:48926-48945 None:intergenic 35.0%
ATAGAATCGCTCTATCTTCT+TGG - tig0018386:49080-49099 None:intergenic 35.0%
GAAGCTTTCAAGCAATTCTT+TGG + tig0018386:49176-49195 Msa1365750:CDS 35.0%
GATCTACTAGAGTAGTTTCT+TGG - tig0018386:49237-49256 None:intergenic 35.0%
AAATGCAAGTTCTTCTTGCA+TGG + tig0018386:49293-49312 Msa1365750:CDS 35.0%
AAGTCTTCAAAACAGTAACC+AGG - tig0018386:49393-49412 None:intergenic 35.0%
AAGAGAAAAACGAGTGTGTT+TGG - tig0018386:48809-48828 None:intergenic 35.0%
CTTGACAGATATCACAAAGA+GGG - tig0018386:48855-48874 None:intergenic 35.0%
ACTTGACAGATATCACAAAG+AGG - tig0018386:48856-48875 None:intergenic 35.0%
TATTAAAGATCCAGATGCGA+CGG - tig0018386:48926-48945 None:intergenic 35.0%
ATAGAATCGCTCTATCTTCT+TGG - tig0018386:49080-49099 None:intergenic 35.0%
GAAGCTTTCAAGCAATTCTT+TGG + tig0018386:49176-49195 Msa1365750:CDS 35.0%
GATCTACTAGAGTAGTTTCT+TGG - tig0018386:49237-49256 None:intergenic 35.0%
AAATGCAAGTTCTTCTTGCA+TGG + tig0018386:49293-49312 Msa1365750:CDS 35.0%
AAGTCTTCAAAACAGTAACC+AGG - tig0018386:49393-49412 None:intergenic 35.0%
CTTTCTCACACACATCACAT+TGG - tig0018386:48699-48718 None:intergenic 40.0%
TTCATCTGAAGGACAGAACA+TGG - tig0018386:48731-48750 None:intergenic 40.0%
GGATTGTATGATCACATCCA+TGG - tig0018386:48765-48784 None:intergenic 40.0%
ATCACAAAGAGGGTAGTCTT+TGG - tig0018386:48845-48864 None:intergenic 40.0%
TATAAAAACGGTCGCGGTAA+AGG + tig0018386:48897-48916 Msa1365750:intron 40.0%
! AATATCGTGCGCTTTTGTAC+AGG + tig0018386:49032-49051 Msa1365750:intron 40.0%
TGTTCATTTGCTCCATGGAT+TGG - tig0018386:49120-49139 None:intergenic 40.0%
GTGTATGTTCATTTGCTCCA+TGG - tig0018386:49125-49144 None:intergenic 40.0%
CATAACAGGTTTCTTCTCTC+TGG + tig0018386:49150-49169 Msa1365750:CDS 40.0%
! TACTGGTGGATGATGAATCT+GGG - tig0018386:49203-49222 None:intergenic 40.0%
! ATACTGGTGGATGATGAATC+TGG - tig0018386:49204-49223 None:intergenic 40.0%
GATTCATCATCCACCAGTAT+TGG + tig0018386:49204-49223 Msa1365750:CDS 40.0%
CCTTACAGAAACCATTAGGT+GGG - tig0018386:49431-49450 None:intergenic 40.0%
ACCTTACAGAAACCATTAGG+TGG - tig0018386:49432-49451 None:intergenic 40.0%
CTTTCTCACACACATCACAT+TGG - tig0018386:48699-48718 None:intergenic 40.0%
TTCATCTGAAGGACAGAACA+TGG - tig0018386:48731-48750 None:intergenic 40.0%
GGATTGTATGATCACATCCA+TGG - tig0018386:48765-48784 None:intergenic 40.0%
ATCACAAAGAGGGTAGTCTT+TGG - tig0018386:48845-48864 None:intergenic 40.0%
TATAAAAACGGTCGCGGTAA+AGG + tig0018386:48897-48916 Msa1365750:intron 40.0%
! AATATCGTGCGCTTTTGTAC+AGG + tig0018386:49032-49051 Msa1365750:intron 40.0%
TGTTCATTTGCTCCATGGAT+TGG - tig0018386:49120-49139 None:intergenic 40.0%
GTGTATGTTCATTTGCTCCA+TGG - tig0018386:49125-49144 None:intergenic 40.0%
CATAACAGGTTTCTTCTCTC+TGG + tig0018386:49150-49169 Msa1365750:CDS 40.0%
! TACTGGTGGATGATGAATCT+GGG - tig0018386:49203-49222 None:intergenic 40.0%
! ATACTGGTGGATGATGAATC+TGG - tig0018386:49204-49223 None:intergenic 40.0%
GATTCATCATCCACCAGTAT+TGG + tig0018386:49204-49223 Msa1365750:CDS 40.0%
CCTTACAGAAACCATTAGGT+GGG - tig0018386:49431-49450 None:intergenic 40.0%
ACCTTACAGAAACCATTAGG+TGG - tig0018386:49432-49451 None:intergenic 40.0%
TGATGTGTGTGAGAAAGCTG+AGG + tig0018386:48702-48721 Msa1365750:CDS 45.0%
CAAAGAGCAGCTTCATCTGA+AGG - tig0018386:48742-48761 None:intergenic 45.0%
GTGTTTGGTTGCAAGCTTGT+TGG - tig0018386:48794-48813 None:intergenic 45.0%
GAATGTGACCTTCCAATCCA+TGG + tig0018386:49105-49124 Msa1365750:CDS 45.0%
!! CATTTGCTCCATGGATTGGA+AGG - tig0018386:49116-49135 None:intergenic 45.0%
TCTTGGTTCAGAGCCAATAC+TGG - tig0018386:49220-49239 None:intergenic 45.0%
TTGCATGGCTGAAGACAACA+TGG + tig0018386:49308-49327 Msa1365750:CDS 45.0%
GATGCTTCTTTCCCACCTAA+TGG + tig0018386:49417-49436 Msa1365750:CDS 45.0%
CCCACCTAATGGTTTCTGTA+AGG + tig0018386:49428-49447 Msa1365750:CDS 45.0%
TGATGTGTGTGAGAAAGCTG+AGG + tig0018386:48702-48721 Msa1365750:CDS 45.0%
CAAAGAGCAGCTTCATCTGA+AGG - tig0018386:48742-48761 None:intergenic 45.0%
GTGTTTGGTTGCAAGCTTGT+TGG - tig0018386:48794-48813 None:intergenic 45.0%
GAATGTGACCTTCCAATCCA+TGG + tig0018386:49105-49124 Msa1365750:CDS 45.0%
!! CATTTGCTCCATGGATTGGA+AGG - tig0018386:49116-49135 None:intergenic 45.0%
TCTTGGTTCAGAGCCAATAC+TGG - tig0018386:49220-49239 None:intergenic 45.0%
TTGCATGGCTGAAGACAACA+TGG + tig0018386:49308-49327 Msa1365750:CDS 45.0%
GATGCTTCTTTCCCACCTAA+TGG + tig0018386:49417-49436 Msa1365750:CDS 45.0%
CCCACCTAATGGTTTCTGTA+AGG + tig0018386:49428-49447 Msa1365750:CDS 45.0%
GATGAAGCTGCTCTTTGCCA+TGG + tig0018386:48745-48764 Msa1365750:CDS 50.0%
!! TGGTTGCAAGCTTGTTGGCA+CGG - tig0018386:48789-48808 None:intergenic 50.0%
!! GGTAGTCTTTGGAGTTGAGG+TGG - tig0018386:48834-48853 None:intergenic 50.0%
!! GAGGGTAGTCTTTGGAGTTG+AGG - tig0018386:48837-48856 None:intergenic 50.0%
TGGTTCAGAGCCAATACTGG+TGG - tig0018386:49217-49236 None:intergenic 50.0%
GACAACATGGCTAGTGACAC+TGG + tig0018386:49321-49340 Msa1365750:CDS 50.0%
GATGAAGCTGCTCTTTGCCA+TGG + tig0018386:48745-48764 Msa1365750:CDS 50.0%
!! TGGTTGCAAGCTTGTTGGCA+CGG - tig0018386:48789-48808 None:intergenic 50.0%
!! GGTAGTCTTTGGAGTTGAGG+TGG - tig0018386:48834-48853 None:intergenic 50.0%
!! GAGGGTAGTCTTTGGAGTTG+AGG - tig0018386:48837-48856 None:intergenic 50.0%
TGGTTCAGAGCCAATACTGG+TGG - tig0018386:49217-49236 None:intergenic 50.0%
GACAACATGGCTAGTGACAC+TGG + tig0018386:49321-49340 Msa1365750:CDS 50.0%
!! TTGCAAGCTTGTTGGCACGG+TGG - tig0018386:48786-48805 None:intergenic 55.0%
GTAAAGGTTGCCGTCGCATC+TGG + tig0018386:48913-48932 Msa1365750:intron 55.0%
! CGTGCGCTTTTGTACAGGAG+AGG + tig0018386:49037-49056 Msa1365750:intron 55.0%
! GCTTTTGTACAGGAGAGGCG+TGG + tig0018386:49042-49061 Msa1365750:intron 55.0%
!! TTGCAAGCTTGTTGGCACGG+TGG - tig0018386:48786-48805 None:intergenic 55.0%
GTAAAGGTTGCCGTCGCATC+TGG + tig0018386:48913-48932 Msa1365750:intron 55.0%
! CGTGCGCTTTTGTACAGGAG+AGG + tig0018386:49037-49056 Msa1365750:intron 55.0%
! GCTTTTGTACAGGAGAGGCG+TGG + tig0018386:49042-49061 Msa1365750:intron 55.0%
Chromosome Type Strat End Strand Name
tig0018386 gene 48688 49455 48688 ID=Msa1365750;Name=Msa1365750
tig0018386 mRNA 48688 49455 48688 ID=Msa1365750-mRNA-1;Parent=Msa1365750;Name=Msa1365750-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0|0|1|1|1|2|0|195
tig0018386 exon 48688 48873 48688 ID=Msa1365750-mRNA-1:exon:14760;Parent=Msa1365750-mRNA-1
tig0018386 exon 49054 49455 49054 ID=Msa1365750-mRNA-1:exon:14761;Parent=Msa1365750-mRNA-1
tig0018386 CDS 48688 48873 48688 ID=Msa1365750-mRNA-1:cds;Parent=Msa1365750-mRNA-1
tig0018386 CDS 49054 49455 49054 ID=Msa1365750-mRNA-1:cds;Parent=Msa1365750-mRNA-1
Gene Sequence

>Msa1365750

ATGAAGATCCAATGTGATGTGTGTGAGAAAGCTGAGGCTTCCATGTTCTGTCCTTCAGATGAAGCTGCTCTTTGCCATGGATGTGATCATACAATCCACCGTGCCAACAAGCTTGCAACCAAACACACTCGTTTTTCTCTTGTCCACCTCAACTCCAAAGACTACCCTCTTTGTGATATCTGTCAAGAGAGGCGTGGATATCTATTTTGCCAAGAAGATAGAGCGATTCTATGCAGAGAATGTGACCTTCCAATCCATGGAGCAAATGAACATACACAGAAACATAACAGGTTTCTTCTCTCTGGTGTGAAGCTTTCAAGCAATTCTTTGGACCCAGATTCATCATCCACCAGTATTGGCTCTGAACCAAGAAACTACTCTAGTAGATCAAAAGCAAACATAATACCAAGATCAATTTCCAATGAAAATGCAAGTTCTTCTTGCATGGCTGAAGACAACATGGCTAGTGACACTGGTTCTGTTTCAACAAGCAGCATTTCTGAATACTTGATTGAAACAATACCTGGTTACTGTTTTGAAGACTTTCTTGATGCTTCTTTCCCACCTAATGGTTTCTGTAAGGTTTAA

Protein sequence

>Msa1365750

MKIQCDVCEKAEASMFCPSDEAALCHGCDHTIHRANKLATKHTRFSLVHLNSKDYPLCDICQERRGYLFCQEDRAILCRECDLPIHGANEHTQKHNRFLLSGVKLSSNSLDPDSSSTSIGSEPRNYSSRSKANIIPRSISNENASSSCMAEDNMASDTGSVSTSSISEYLIETIPGYCFEDFLDASFPPNGFCKV*