AlfalfaGEDB | Msa1366580

M. sativa cultivar zhongmu-4 / Msa1366580

KEGG: -
GO: GO:0000302 GO:0001101 GO:0002831 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006950 GO:0006970 GO:0006979 GO:0007154 GO:0007275 GO:0007568 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009414 GO:0009415 GO:0009416 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009625 GO:0009628 GO:0009636 GO:0009642 GO:0009646 GO:0009651 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010150 GO:0022622 GO:0031347 GO:0031668 GO:0032101 GO:0032501 GO:0032502 GO:0033554 GO:0033993 GO:0042221 GO:0042493 GO:0042542 GO:0042631 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0048364 GO:0048366 GO:0048367 GO:0048580 GO:0048583 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0070887 GO:0071214 GO:0071229 GO:0071462 GO:0071496 GO:0080134 GO:0090693 GO:0097305 GO:0099402 GO:0104004 GO:1900055 GO:1900150 GO:1900424 GO:1901700 GO:1901701 GO:1902074 GO:2000024 GO:2000026 GO:2000028 GO:2000241
PFAM: PF03242.16

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1366580	GAU11234.1	82.474	97	12	1	1	92	1	97	3.93e-50	163

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1366580	sp\|P32292\|ARG2_VIGRR	54.455	101	35	4	1	92	1	99	2.72e-27	98.6

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1366580	A0A2K3LCK8	82.474	97	12	1	1	92	1	97	2.41e-50	163

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0146830	Msa1366580	0.816532	5.269270e-52	-8.615850e-47
Msa1041200	Msa1366580	0.904521	1.124740e-79	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1366580	MtrunA17_Chr7g0269421	82.828	99	10	1	1	92	1	99	2.69e-51	156
Msa1366580	MtrunA17_Chr1g0195391	54.839	93	39	3	1	92	1	91	1.99e-24	88.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1366580	AT4G02380.1	44.898	98	43	3	1	89	1	96	3.19e-17	70.5
Msa1366580	AT1G02820.1	47.826	92	43	3	1	89	1	90	4.72e-17	69.7
Msa1366580	AT4G02380.3	44.898	98	43	3	1	89	96	191	3.30e-16	70.1

Find 57 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTATATTATATTATGTATTT+TGG	0.081551	tig0018459:-129144	Msa1366580:three_prime_UTR
ACTCTTTCATTCATTCTTTC+GGG	0.201868	tig0018459:-129707	Msa1366580:five_prime_UTR
AACTCTTTCATTCATTCTTT+CGG	0.219672	tig0018459:-129708	Msa1366580:five_prime_UTR
CGGTGGATCTCATGAGCTTT+TGG	0.249055	tig0018459:-129107	Msa1366580:three_prime_UTR
CATTCATTCTTTCGGGCTTA+AGG	0.288947	tig0018459:-129700	Msa1366580:five_prime_UTR
AGATGGTGCTTCGACTTATA+AGG	0.290073	tig0018459:-129301	Msa1366580:CDS
TACATCTTACACTATTAGTA+TGG	0.328608	tig0018459:-129576	Msa1366580:exon
TTCTCTGCTATTGTCCTTGA+AGG	0.342980	tig0018459:-129527	Msa1366580:CDS
CTTCGACTTATAAGGTTTCA+TGG	0.345319	tig0018459:-129293	Msa1366580:CDS
AAGATGGGACCCACAAAATC+AGG	0.353277	tig0018459:-129327	Msa1366580:CDS
CTATAAGCCTGAGAATATTA+AGG	0.363636	tig0018459:-129247	Msa1366580:CDS
GAATTGTTTGTGAGAGGAAT+AGG	0.388357	tig0018459:+129739	None:intergenic
TAGTAACCAGTGACGGGATC+TGG	0.403824	tig0018459:+129267	None:intergenic
AAGATGTATTAAATGAACTA+TGG	0.405251	tig0018459:+129591	None:intergenic
TCAACAAAGTTGTCATCAAA+TGG	0.417779	tig0018459:+128819	None:intergenic
TCAGTATCCTTAATATTCTC+AGG	0.439065	tig0018459:+129240	None:intergenic
TCGTTCTTTCGCTAACGCTA+AGG	0.475487	tig0018459:-129552	Msa1366580:CDS
ACGTGGTTACGCTGCGGCAA+GGG	0.477009	tig0018459:-129373	Msa1366580:CDS
CCCACAAAATCAGGGGAAGA+TGG	0.498300	tig0018459:-129318	Msa1366580:CDS
CGCTGCGGCAAGGGGAGGAG+TGG	0.505897	tig0018459:-129364	Msa1366580:CDS
GCAACTAATCATCATTCCTC+TGG	0.509131	tig0018459:+128969	None:intergenic
AAAATTGAATTGTTTGTGAG+AGG	0.509614	tig0018459:+129733	None:intergenic
TTGCGAAGATCCTACTCTGT+TGG	0.512688	tig0018459:-129035	Msa1366580:three_prime_UTR
TGGGTTCCAGATCCCGTCAC+TGG	0.514530	tig0018459:-129273	Msa1366580:CDS
TTCGACTTATAAGGTTTCAT+GGG	0.523405	tig0018459:-129292	Msa1366580:CDS
TTAACAGAATCAATCAATCA+AGG	0.537130	tig0018459:+129058	None:intergenic
AGGGGAGGAGTGGTGTCTGT+TGG	0.549168	tig0018459:-129354	Msa1366580:CDS
GAGGGTTGGAGAATCCTTCA+AGG	0.550131	tig0018459:+129513	None:intergenic
GAAGATCCTACTCTGTTGGT+TGG	0.557582	tig0018459:-129031	Msa1366580:three_prime_UTR
GACGTGGTTACGCTGCGGCA+AGG	0.563935	tig0018459:-129374	Msa1366580:CDS
ACGGTACCTTGTGAGAGGGT+TGG	0.566085	tig0018459:+129499	None:intergenic
GTGGAGGACTATATATAATG+TGG	0.566119	tig0018459:+129765	None:intergenic
AGATGGGACCCACAAAATCA+GGG	0.576868	tig0018459:-129326	Msa1366580:CDS
TCATTCTTTCGGGCTTAAGG+TGG	0.577395	tig0018459:-129697	Msa1366580:five_prime_UTR
CGTGGTTACGCTGCGGCAAG+GGG	0.578056	tig0018459:-129372	Msa1366580:CDS
TGAGAGGAATAGGTGAGTGG+AGG	0.581220	tig0018459:+129749	None:intergenic
GGCTTATAGTAACCAGTGAC+GGG	0.583700	tig0018459:+129261	None:intergenic
TGGGTTTGTTATTTAAACGG+TGG	0.588214	tig0018459:-129124	Msa1366580:three_prime_UTR
TATAATGTGGAGAAAAGAGA+GGG	0.590762	tig0018459:+129778	None:intergenic
AGGCTTATAGTAACCAGTGA+CGG	0.594196	tig0018459:+129260	None:intergenic
GGTGTCTGTTGGCAACAAGA+TGG	0.594809	tig0018459:-129343	Msa1366580:CDS
GTTGCTGATCATAAGTGTAA+TGG	0.599895	tig0018459:-128951	Msa1366580:three_prime_UTR
GGTTACGCTGCGGCAAGGGG+AGG	0.607716	tig0018459:-129369	Msa1366580:CDS
GTGTCTGTTGGCAACAAGAT+GGG	0.610680	tig0018459:-129342	Msa1366580:CDS
ACGTTACGGTACCTTGTGAG+AGG	0.618071	tig0018459:+129494	None:intergenic
TTGTGAGAGGAATAGGTGAG+TGG	0.618295	tig0018459:+129746	None:intergenic
GGAGAAAAGAGAGGGCAAAG+AGG	0.637158	tig0018459:+129786	None:intergenic
AATGAACCAACCAACAGAGT+AGG	0.638709	tig0018459:+129025	None:intergenic
CGTTACGGTACCTTGTGAGA+GGG	0.641245	tig0018459:+129495	None:intergenic
ATATAATGTGGAGAAAAGAG+AGG	0.652732	tig0018459:+129777	None:intergenic
CATAACGATAGTTTAAACTG+TGG	0.657037	tig0018459:-129665	Msa1366580:five_prime_UTR
GATTCTCCAACCCTCTCACA+AGG	0.659794	tig0018459:-129505	Msa1366580:intron
AAGCTTAGCACGTAAATCAG+CGG	0.682540	tig0018459:+129215	None:intergenic
GATGGGACCCACAAAATCAG+GGG	0.703081	tig0018459:-129325	Msa1366580:CDS
TGATCATAAGTGTAATGGTG+TGG	0.712483	tig0018459:-128946	Msa1366580:three_prime_UTR
CTAACAAAGTAAATAAACAG+AGG	0.722024	tig0018459:+129002	None:intergenic
AAACAGACGTGGTTACGCTG+CGG	0.738749	tig0018459:-129379	Msa1366580:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTATTTTTAATTAATAAT+TGG	-	tig0018459:129722-129741	Msa1366580:five_prime_UTR	0.0%
!!!	TTTTATTTTTAATTAATAAT+TGG	-	tig0018459:129722-129741	Msa1366580:five_prime_UTR	0.0%
!!!	TTTTAATTAATAATTGGTTT+AGG	-	tig0018459:129728-129747	Msa1366580:five_prime_UTR	10.0%
!!!	TTTTAATTAATAATTGGTTT+AGG	-	tig0018459:129728-129747	Msa1366580:five_prime_UTR	10.0%
!!!	TTATTTACTTTGTTAGTTTT+TGG	-	tig0018459:129621-129640	Msa1366580:five_prime_UTR	15.0%
!!!	TATTTACTTTGTTAGTTTTT+GGG	-	tig0018459:129622-129641	Msa1366580:five_prime_UTR	15.0%
!!!	TTATTTACTTTGTTAGTTTT+TGG	-	tig0018459:129621-129640	Msa1366580:five_prime_UTR	15.0%
!!!	TATTTACTTTGTTAGTTTTT+GGG	-	tig0018459:129622-129641	Msa1366580:five_prime_UTR	15.0%
!!	AAGATGTATTAAATGAACTA+TGG	+	tig0018459:129028-129047	None:intergenic	20.0%
!!	AATAACAATTAGAAACGTTA+CGG	+	tig0018459:129139-129158	None:intergenic	20.0%
!!!	ATATCTGAGATTTTAACATA+TGG	-	tig0018459:129199-129218	Msa1366580:CDS	20.0%
!!	TTAAAATAATTAAACAGACG+TGG	-	tig0018459:129226-129245	Msa1366580:CDS	20.0%
!!!	ATTAGTTGTTGAATTTTTTG+CGG	+	tig0018459:129429-129448	None:intergenic	20.0%
!!!	TTTTGGGTTTGTTATTTAAA+CGG	-	tig0018459:129489-129508	Msa1366580:intron	20.0%
!!	AAGATGTATTAAATGAACTA+TGG	+	tig0018459:129028-129047	None:intergenic	20.0%
!!	AATAACAATTAGAAACGTTA+CGG	+	tig0018459:129139-129158	None:intergenic	20.0%
!!!	ATATCTGAGATTTTAACATA+TGG	-	tig0018459:129199-129218	Msa1366580:CDS	20.0%
!!	TTAAAATAATTAAACAGACG+TGG	-	tig0018459:129226-129245	Msa1366580:CDS	20.0%
!!!	ATTAGTTGTTGAATTTTTTG+CGG	+	tig0018459:129429-129448	None:intergenic	20.0%
!!!	TTTTGGGTTTGTTATTTAAA+CGG	-	tig0018459:129489-129508	Msa1366580:intron	20.0%
!	AAAATTGAATTGTTTGTGAG+AGG	+	tig0018459:128886-128905	None:intergenic	25.0%
!	AACTCTTTCATTCATTCTTT+CGG	-	tig0018459:128908-128927	Msa1366580:three_prime_UTR	25.0%
!	TACATCTTACACTATTAGTA+TGG	-	tig0018459:129040-129059	Msa1366580:three_prime_UTR	25.0%
!	TTAACAGAATCAATCAATCA+AGG	+	tig0018459:129561-129580	None:intergenic	25.0%
!	CTAACAAAGTAAATAAACAG+AGG	+	tig0018459:129617-129636	None:intergenic	25.0%
!	AAAATTGAATTGTTTGTGAG+AGG	+	tig0018459:128886-128905	None:intergenic	25.0%
!	AACTCTTTCATTCATTCTTT+CGG	-	tig0018459:128908-128927	Msa1366580:three_prime_UTR	25.0%
!	TACATCTTACACTATTAGTA+TGG	-	tig0018459:129040-129059	Msa1366580:three_prime_UTR	25.0%
!	TTAACAGAATCAATCAATCA+AGG	+	tig0018459:129561-129580	None:intergenic	25.0%
!	CTAACAAAGTAAATAAACAG+AGG	+	tig0018459:129617-129636	None:intergenic	25.0%
	TATAATGTGGAGAAAAGAGA+GGG	+	tig0018459:128841-128860	None:intergenic	30.0%
	ATATAATGTGGAGAAAAGAG+AGG	+	tig0018459:128842-128861	None:intergenic	30.0%
	ACTCTTTCATTCATTCTTTC+GGG	-	tig0018459:128909-128928	Msa1366580:three_prime_UTR	30.0%
	CATAACGATAGTTTAAACTG+TGG	-	tig0018459:128951-128970	Msa1366580:three_prime_UTR	30.0%
	TTCGACTTATAAGGTTTCAT+GGG	-	tig0018459:129324-129343	Msa1366580:CDS	30.0%
	CTATAAGCCTGAGAATATTA+AGG	-	tig0018459:129369-129388	Msa1366580:CDS	30.0%
	TCAGTATCCTTAATATTCTC+AGG	+	tig0018459:129379-129398	None:intergenic	30.0%
	TATAATGTGGAGAAAAGAGA+GGG	+	tig0018459:128841-128860	None:intergenic	30.0%
	ATATAATGTGGAGAAAAGAG+AGG	+	tig0018459:128842-128861	None:intergenic	30.0%
	ACTCTTTCATTCATTCTTTC+GGG	-	tig0018459:128909-128928	Msa1366580:three_prime_UTR	30.0%
	CATAACGATAGTTTAAACTG+TGG	-	tig0018459:128951-128970	Msa1366580:three_prime_UTR	30.0%
	TTCGACTTATAAGGTTTCAT+GGG	-	tig0018459:129324-129343	Msa1366580:CDS	30.0%
	CTATAAGCCTGAGAATATTA+AGG	-	tig0018459:129369-129388	Msa1366580:CDS	30.0%
	TCAGTATCCTTAATATTCTC+AGG	+	tig0018459:129379-129398	None:intergenic	30.0%
	GTGGAGGACTATATATAATG+TGG	+	tig0018459:128854-128873	None:intergenic	35.0%
	GAATTGTTTGTGAGAGGAAT+AGG	+	tig0018459:128880-128899	None:intergenic	35.0%
	CTTCGACTTATAAGGTTTCA+TGG	-	tig0018459:129323-129342	Msa1366580:CDS	35.0%
!!	TGGGTTTGTTATTTAAACGG+TGG	-	tig0018459:129492-129511	Msa1366580:intron	35.0%
!!!	CTTTTGGGATCGATTTTAGA+TGG	-	tig0018459:129525-129544	Msa1366580:CDS	35.0%
	GTTGCTGATCATAAGTGTAA+TGG	-	tig0018459:129665-129684	Msa1366580:five_prime_UTR	35.0%
	TGATCATAAGTGTAATGGTG+TGG	-	tig0018459:129670-129689	Msa1366580:five_prime_UTR	35.0%
!	CATCCATTTGATGACAACTT+TGG	-	tig0018459:129794-129813	Msa1366580:five_prime_UTR	35.0%
	GTGGAGGACTATATATAATG+TGG	+	tig0018459:128854-128873	None:intergenic	35.0%
	GAATTGTTTGTGAGAGGAAT+AGG	+	tig0018459:128880-128899	None:intergenic	35.0%
	CTTCGACTTATAAGGTTTCA+TGG	-	tig0018459:129323-129342	Msa1366580:CDS	35.0%
!!	TGGGTTTGTTATTTAAACGG+TGG	-	tig0018459:129492-129511	Msa1366580:intron	35.0%
!!!	CTTTTGGGATCGATTTTAGA+TGG	-	tig0018459:129525-129544	Msa1366580:CDS	35.0%
	GTTGCTGATCATAAGTGTAA+TGG	-	tig0018459:129665-129684	Msa1366580:five_prime_UTR	35.0%
	TGATCATAAGTGTAATGGTG+TGG	-	tig0018459:129670-129689	Msa1366580:five_prime_UTR	35.0%
	CATTCATTCTTTCGGGCTTA+AGG	-	tig0018459:128916-128935	Msa1366580:three_prime_UTR	40.0%
	TTCTCTGCTATTGTCCTTGA+AGG	-	tig0018459:129089-129108	Msa1366580:three_prime_UTR	40.0%
	AGATGGTGCTTCGACTTATA+AGG	-	tig0018459:129315-129334	Msa1366580:CDS	40.0%
	AGGCTTATAGTAACCAGTGA+CGG	+	tig0018459:129359-129378	None:intergenic	40.0%
	AAGCTTAGCACGTAAATCAG+CGG	+	tig0018459:129404-129423	None:intergenic	40.0%
	AATGAACCAACCAACAGAGT+AGG	+	tig0018459:129594-129613	None:intergenic	40.0%
!!!	TGTTAGTTTTTGGGAACCAG+AGG	-	tig0018459:129631-129650	Msa1366580:five_prime_UTR	40.0%
	GCAACTAATCATCATTCCTC+TGG	+	tig0018459:129650-129669	None:intergenic	40.0%
	CATTCATTCTTTCGGGCTTA+AGG	-	tig0018459:128916-128935	Msa1366580:three_prime_UTR	40.0%
	TTCTCTGCTATTGTCCTTGA+AGG	-	tig0018459:129089-129108	Msa1366580:three_prime_UTR	40.0%
	AGATGGTGCTTCGACTTATA+AGG	-	tig0018459:129315-129334	Msa1366580:CDS	40.0%
	AGGCTTATAGTAACCAGTGA+CGG	+	tig0018459:129359-129378	None:intergenic	40.0%
	AAGCTTAGCACGTAAATCAG+CGG	+	tig0018459:129404-129423	None:intergenic	40.0%
	AATGAACCAACCAACAGAGT+AGG	+	tig0018459:129594-129613	None:intergenic	40.0%
!!!	TGTTAGTTTTTGGGAACCAG+AGG	-	tig0018459:129631-129650	Msa1366580:five_prime_UTR	40.0%
	GCAACTAATCATCATTCCTC+TGG	+	tig0018459:129650-129669	None:intergenic	40.0%
	TTGTGAGAGGAATAGGTGAG+TGG	+	tig0018459:128873-128892	None:intergenic	45.0%
	TCATTCTTTCGGGCTTAAGG+TGG	-	tig0018459:128919-128938	Msa1366580:three_prime_UTR	45.0%
	TCGTTCTTTCGCTAACGCTA+AGG	-	tig0018459:129064-129083	Msa1366580:three_prime_UTR	45.0%
	GTGTCTGTTGGCAACAAGAT+GGG	-	tig0018459:129274-129293	Msa1366580:CDS	45.0%
	AAGATGGGACCCACAAAATC+AGG	-	tig0018459:129289-129308	Msa1366580:CDS	45.0%
	AGATGGGACCCACAAAATCA+GGG	-	tig0018459:129290-129309	Msa1366580:CDS	45.0%
!	CCATCTTCCCCTGATTTTGT+GGG	+	tig0018459:129301-129320	None:intergenic	45.0%
!	ACCATCTTCCCCTGATTTTG+TGG	+	tig0018459:129302-129321	None:intergenic	45.0%
	GGCTTATAGTAACCAGTGAC+GGG	+	tig0018459:129358-129377	None:intergenic	45.0%
!	GGTGGATCTCATGAGCTTTT+GGG	-	tig0018459:129510-129529	Msa1366580:CDS	45.0%
	TTGCGAAGATCCTACTCTGT+TGG	-	tig0018459:129581-129600	Msa1366580:five_prime_UTR	45.0%
	GAAGATCCTACTCTGTTGGT+TGG	-	tig0018459:129585-129604	Msa1366580:five_prime_UTR	45.0%
	TTGTGAGAGGAATAGGTGAG+TGG	+	tig0018459:128873-128892	None:intergenic	45.0%
	TCATTCTTTCGGGCTTAAGG+TGG	-	tig0018459:128919-128938	Msa1366580:three_prime_UTR	45.0%
	TCGTTCTTTCGCTAACGCTA+AGG	-	tig0018459:129064-129083	Msa1366580:three_prime_UTR	45.0%
	GTGTCTGTTGGCAACAAGAT+GGG	-	tig0018459:129274-129293	Msa1366580:CDS	45.0%
	AAGATGGGACCCACAAAATC+AGG	-	tig0018459:129289-129308	Msa1366580:CDS	45.0%
	AGATGGGACCCACAAAATCA+GGG	-	tig0018459:129290-129309	Msa1366580:CDS	45.0%
!	CCATCTTCCCCTGATTTTGT+GGG	+	tig0018459:129301-129320	None:intergenic	45.0%
!	ACCATCTTCCCCTGATTTTG+TGG	+	tig0018459:129302-129321	None:intergenic	45.0%
	GGCTTATAGTAACCAGTGAC+GGG	+	tig0018459:129358-129377	None:intergenic	45.0%
!	GGTGGATCTCATGAGCTTTT+GGG	-	tig0018459:129510-129529	Msa1366580:CDS	45.0%
	TTGCGAAGATCCTACTCTGT+TGG	-	tig0018459:129581-129600	Msa1366580:five_prime_UTR	45.0%
	GAAGATCCTACTCTGTTGGT+TGG	-	tig0018459:129585-129604	Msa1366580:five_prime_UTR	45.0%
!!!	TTATATTATATTATGTATTT+TGG	-	tig0018459:129472-129491	Msa1366580:intron	5.0%
!!!	TATATTATATTATGTATTTT+GGG	-	tig0018459:129473-129492	Msa1366580:intron	5.0%
!!!	TTATATTATATTATGTATTT+TGG	-	tig0018459:129472-129491	Msa1366580:intron	5.0%
!!!	TATATTATATTATGTATTTT+GGG	-	tig0018459:129473-129492	Msa1366580:intron	5.0%
	GGAGAAAAGAGAGGGCAAAG+AGG	+	tig0018459:128833-128852	None:intergenic	50.0%
	TGAGAGGAATAGGTGAGTGG+AGG	+	tig0018459:128870-128889	None:intergenic	50.0%
	GAGGGTTGGAGAATCCTTCA+AGG	+	tig0018459:129106-129125	None:intergenic	50.0%
	GATTCTCCAACCCTCTCACA+AGG	-	tig0018459:129111-129130	Msa1366580:three_prime_UTR	50.0%
	CGTTACGGTACCTTGTGAGA+GGG	+	tig0018459:129124-129143	None:intergenic	50.0%
	ACGTTACGGTACCTTGTGAG+AGG	+	tig0018459:129125-129144	None:intergenic	50.0%
	AAACAGACGTGGTTACGCTG+CGG	-	tig0018459:129237-129256	Msa1366580:CDS	50.0%
	GGTGTCTGTTGGCAACAAGA+TGG	-	tig0018459:129273-129292	Msa1366580:CDS	50.0%
	GATGGGACCCACAAAATCAG+GGG	-	tig0018459:129291-129310	Msa1366580:CDS	50.0%
	CCCACAAAATCAGGGGAAGA+TGG	-	tig0018459:129298-129317	Msa1366580:CDS	50.0%
!	TAGTAACCAGTGACGGGATC+TGG	+	tig0018459:129352-129371	None:intergenic	50.0%
!	CGGTGGATCTCATGAGCTTT+TGG	-	tig0018459:129509-129528	Msa1366580:CDS	50.0%
	GGAGAAAAGAGAGGGCAAAG+AGG	+	tig0018459:128833-128852	None:intergenic	50.0%
	TGAGAGGAATAGGTGAGTGG+AGG	+	tig0018459:128870-128889	None:intergenic	50.0%
	GAGGGTTGGAGAATCCTTCA+AGG	+	tig0018459:129106-129125	None:intergenic	50.0%
	GATTCTCCAACCCTCTCACA+AGG	-	tig0018459:129111-129130	Msa1366580:three_prime_UTR	50.0%
	CGTTACGGTACCTTGTGAGA+GGG	+	tig0018459:129124-129143	None:intergenic	50.0%
	ACGTTACGGTACCTTGTGAG+AGG	+	tig0018459:129125-129144	None:intergenic	50.0%
	AAACAGACGTGGTTACGCTG+CGG	-	tig0018459:129237-129256	Msa1366580:CDS	50.0%
	GGTGTCTGTTGGCAACAAGA+TGG	-	tig0018459:129273-129292	Msa1366580:CDS	50.0%
	GATGGGACCCACAAAATCAG+GGG	-	tig0018459:129291-129310	Msa1366580:CDS	50.0%
	CCCACAAAATCAGGGGAAGA+TGG	-	tig0018459:129298-129317	Msa1366580:CDS	50.0%
!	TAGTAACCAGTGACGGGATC+TGG	+	tig0018459:129352-129371	None:intergenic	50.0%
!	CGGTGGATCTCATGAGCTTT+TGG	-	tig0018459:129509-129528	Msa1366580:CDS	50.0%
	ACGGTACCTTGTGAGAGGGT+TGG	+	tig0018459:129120-129139	None:intergenic	55.0%
	ACGGTACCTTGTGAGAGGGT+TGG	+	tig0018459:129120-129139	None:intergenic	55.0%
	ACGTGGTTACGCTGCGGCAA+GGG	-	tig0018459:129243-129262	Msa1366580:CDS	60.0%
	AGGGGAGGAGTGGTGTCTGT+TGG	-	tig0018459:129262-129281	Msa1366580:CDS	60.0%
	TGGGTTCCAGATCCCGTCAC+TGG	-	tig0018459:129343-129362	Msa1366580:CDS	60.0%
	ACGTGGTTACGCTGCGGCAA+GGG	-	tig0018459:129243-129262	Msa1366580:CDS	60.0%
	AGGGGAGGAGTGGTGTCTGT+TGG	-	tig0018459:129262-129281	Msa1366580:CDS	60.0%
	TGGGTTCCAGATCCCGTCAC+TGG	-	tig0018459:129343-129362	Msa1366580:CDS	60.0%
	GACGTGGTTACGCTGCGGCA+AGG	-	tig0018459:129242-129261	Msa1366580:CDS	65.0%
	CGTGGTTACGCTGCGGCAAG+GGG	-	tig0018459:129244-129263	Msa1366580:CDS	65.0%
	GACGTGGTTACGCTGCGGCA+AGG	-	tig0018459:129242-129261	Msa1366580:CDS	65.0%
	CGTGGTTACGCTGCGGCAAG+GGG	-	tig0018459:129244-129263	Msa1366580:CDS	65.0%
	GGTTACGCTGCGGCAAGGGG+AGG	-	tig0018459:129247-129266	Msa1366580:CDS	70.0%
	GGTTACGCTGCGGCAAGGGG+AGG	-	tig0018459:129247-129266	Msa1366580:CDS	70.0%
	CGCTGCGGCAAGGGGAGGAG+TGG	-	tig0018459:129252-129271	Msa1366580:CDS	75.0%
	CGCTGCGGCAAGGGGAGGAG+TGG	-	tig0018459:129252-129271	Msa1366580:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
tig0018459	gene	128823	129815	128823	ID=Msa1366580;Name=Msa1366580
tig0018459	mRNA	128823	129815	128823	ID=Msa1366580-mRNA-1;Parent=Msa1366580;Name=Msa1366580-mRNA-1;_AED=0.16;_eAED=0.16;_QI=236\|1\|1\|1\|1\|1\|2\|368\|92
tig0018459	exon	128823	129395	128823	ID=Msa1366580-mRNA-1:exon:15184;Parent=Msa1366580-mRNA-1
tig0018459	exon	129506	129815	129506	ID=Msa1366580-mRNA-1:exon:15183;Parent=Msa1366580-mRNA-1
tig0018459	five_prime_UTR	129580	129815	129580	ID=Msa1366580-mRNA-1:five_prime_utr;Parent=Msa1366580-mRNA-1
tig0018459	CDS	129506	129579	129506	ID=Msa1366580-mRNA-1:cds;Parent=Msa1366580-mRNA-1
tig0018459	CDS	129191	129395	129191	ID=Msa1366580-mRNA-1:cds;Parent=Msa1366580-mRNA-1
tig0018459	three_prime_UTR	128823	129190	128823	ID=Msa1366580-mRNA-1:three_prime_utr;Parent=Msa1366580-mRNA-1

Gene Sequence	>Msa1366580 ATGGCTCGTTCTTTCGCTAACGCTAAGGCTTTCTCTGCTATTGTCCTTGAAGGATTCTCCAACCCTCTCACAAGACGTGGTTACGCTGCGGCAAGGGGAGGAGTGGTGTCTGTTGGCAACAAGATGGGACCCACAAAATCAGGGGAAGATGGTGCTTCGACTTATAAGGTTTCATGGGTTCCAGATCCCGTCACTGGTTACTATAAGCCTGAGAATATTAAGGATACTGATGCCGCTGATTTACGTGCTAAGCTTCTCCGCAAAAAATTCAACAACTAA
Protein sequence	>Msa1366580 MARSFANAKAFSAIVLEGFSNPLTRRGYAAARGGVVSVGNKMGPTKSGEDGASTYKVSWVPDPVTGYYKPENIKDTDAADLRAKLLRKKFNN*