AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1379140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1379140 MtrunA17_Chr3g0115211 63.793 232 41 3 62 250 892 1123 7.02e-86 278
Msa1379140 MtrunA17_Chr8g0335041 58.763 97 12 2 137 207 1 95 7.92e-26 97.4
Msa1379140 MtrunA17_Chr3g0131711 90.909 33 3 0 42 74 156 188 1.64e-11 63.9
Msa1379140 MtrunA17_Chr8g0385811 90.909 33 3 0 42 74 160 192 1.64e-11 63.9
Msa1379140 MtrunA17_Chr3g0134631 90.909 33 3 0 42 74 161 193 1.64e-11 63.9
Msa1379140 MtrunA17_Chr3g0135231 90.909 33 3 0 42 74 147 179 4.74e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1379140 AT1G77800.3 38.182 220 88 8 72 250 875 1087 3.63e-30 119
Msa1379140 AT1G77800.4 38.182 220 88 8 72 250 875 1087 3.91e-30 119
Msa1379140 AT1G77800.1 38.182 220 88 8 72 250 825 1037 4.24e-30 119
Msa1379140 AT1G77800.2 38.182 220 88 8 72 250 825 1037 4.32e-30 119
Msa1379140 AT1G77800.5 38.182 220 88 8 72 250 875 1087 4.40e-30 118
Msa1379140 AT1G77800.6 38.182 220 88 8 72 250 848 1060 4.79e-30 118
Msa1379140 AT1G77800.7 38.182 220 88 8 72 250 806 1018 5.29e-30 118

Find 86 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCAAATTTGCCTAGTATT+TGG 0.151147 tig0020277:-23212 None:intergenic
AATTAGATGGAGGGAAATTT+TGG 0.174206 tig0020277:-20381 None:intergenic
TGACAATAATCTATCTATTT+AGG 0.192693 tig0020277:+23017 Msa1379140:three_prime_UTR
GGCCATCTGCCATAAATAAT+TGG 0.197244 tig0020277:+22555 Msa1379140:CDS
TGGCTTCTCCCAATTATTTA+TGG 0.197593 tig0020277:-22564 None:intergenic
AGCCAAATACTAGGCAAATT+TGG 0.206453 tig0020277:+23210 Msa1379140:three_prime_UTR
CTGCTTTGCGTGTGTGTATT+TGG 0.249599 tig0020277:-20614 None:intergenic
GCACAAAATCTGCTAGTATT+TGG 0.260441 tig0020277:-23243 None:intergenic
GGCAGATGGCCCAGAACTTC+TGG 0.261026 tig0020277:-22543 None:intergenic
AAAGAGAAGAGAGAAAGTTT+TGG 0.279987 tig0020277:-20544 None:intergenic
GATCTATTGTCCAGAAGTTC+TGG 0.291520 tig0020277:+22533 Msa1379140:CDS
TCCGCGTGCCGAGAGAGTTT+TGG 0.296686 tig0020277:+21574 Msa1379140:CDS
CATCGCCGCGAGAGCACTTC+TGG 0.315808 tig0020277:-20750 None:intergenic
GCACATTCTACAACAAAATA+TGG 0.320317 tig0020277:-22584 None:intergenic
AACTCTAGAAAGAGTCTCTT+TGG 0.352202 tig0020277:-22215 None:intergenic
GTCTCTTTGGCACGGGGCAT+TGG 0.373676 tig0020277:-22202 None:intergenic
TAGAGGGGTAAAGATATATT+CGG 0.381146 tig0020277:+22882 Msa1379140:three_prime_UTR
GCTCTTTGTGGTGGTACTAC+TGG 0.402180 tig0020277:+22605 Msa1379140:CDS
TTGAAGTTGGATGCCTTAAG+TGG 0.416921 tig0020277:+22157 Msa1379140:CDS
CCTTGCAACCAGAGCATAGT+AGG 0.420383 tig0020277:-22360 None:intergenic
GGTAGTCATCTGATGGTCAA+TGG 0.424003 tig0020277:+22636 Msa1379140:exon
CAGAGTTAAACGTAATTTCA+AGG 0.426357 tig0020277:-20695 None:intergenic
CTGTTAATTGGAAGGGGCAG+TGG 0.432063 tig0020277:+21524 Msa1379140:CDS
CTGCTACAAAGTCTCTTTCA+CGG 0.442513 tig0020277:+20647 Msa1379140:five_prime_UTR
ATCGAAGGAGATAAAACAAA+TGG 0.448417 tig0020277:-20489 None:intergenic
TATGAACTTATAGGTTCTGT+TGG 0.451456 tig0020277:+22852 Msa1379140:intron
GTTCTGTTGGAGATATATAG+AGG 0.453158 tig0020277:+22865 Msa1379140:three_prime_UTR
CGGGGCATTGGCTGAGAACA+TGG 0.461925 tig0020277:-22190 None:intergenic
ATAGAGAGATAGAATGAGGA+TGG 0.467266 tig0020277:-20338 None:intergenic
GGTTCATGCCTTCTGTGCTC+AGG 0.470096 tig0020277:+22658 Msa1379140:three_prime_UTR
AATCTCCTGGGGCAAACTCT+TGG 0.470177 tig0020277:-21975 None:intergenic
AAGAGAGAGAAGGGGATCGA+AGG 0.470935 tig0020277:-20504 None:intergenic
GCCATCTGCCATAAATAATT+GGG 0.472144 tig0020277:+22556 Msa1379140:CDS
TGGACCAGTCCGCCCACTTA+AGG 0.473529 tig0020277:-22170 None:intergenic
TCCCAATTATTTATGGCAGA+TGG 0.474546 tig0020277:-22557 None:intergenic
GAAATTGCAAAAGAAGTAAG+TGG 0.476640 tig0020277:-22081 None:intergenic
ATAAAACACATCAACAAAGA+TGG 0.489693 tig0020277:+21999 Msa1379140:CDS
CAGAAGTGCTCTCGCGGCGA+TGG 0.493289 tig0020277:+20751 Msa1379140:five_prime_UTR
CCTACTATGCTCTGGTTGCA+AGG 0.501390 tig0020277:+22360 Msa1379140:CDS
TCTATTTAGGCTGGCTGTGG+AGG 0.501898 tig0020277:+23030 Msa1379140:three_prime_UTR
GTTGTAGAATGTGCTCTTTG+TGG 0.503308 tig0020277:+22593 Msa1379140:CDS
GTAGTCATCTGATGGTCAAT+GGG 0.504537 tig0020277:+22637 Msa1379140:exon
TTGTCTTTCACCTGCTCAGA+AGG 0.505332 tig0020277:-20892 None:intergenic
ATCTATTGTCCAGAAGTTCT+GGG 0.505690 tig0020277:+22534 Msa1379140:CDS
CAACCAGAGCATAGTAGGAT+AGG 0.511552 tig0020277:-22355 None:intergenic
TGAAGAGATAGCGCCGTCGC+AGG 0.513649 tig0020277:-20718 None:intergenic
TCTGTTGAAGATATATAAGA+TGG 0.514275 tig0020277:+22907 Msa1379140:three_prime_UTR
TGTTGCCAAGAGTTTGCCCC+AGG 0.516719 tig0020277:+21970 Msa1379140:CDS
CTTGATCTTAGAGAGGCAAC+AGG 0.517782 tig0020277:+22049 Msa1379140:CDS
AGAAAGAGTCTCTTTGGCAC+GGG 0.519668 tig0020277:-22209 None:intergenic
TTGGAGGAAGCTCGTCAACT+TGG 0.522024 tig0020277:+21605 Msa1379140:CDS
CTATCTATTTAGGCTGGCTG+TGG 0.523471 tig0020277:+23027 Msa1379140:three_prime_UTR
TTCTGTTGGAGATATATAGA+GGG 0.524248 tig0020277:+22866 Msa1379140:three_prime_UTR
AATCCTATCCTACTATGCTC+TGG 0.524276 tig0020277:+22352 Msa1379140:CDS
TGAGATAGAGAGATAGAATG+AGG 0.524760 tig0020277:-20342 None:intergenic
CGTAGTGTGAAAGAAACAAC+CGG 0.529590 tig0020277:+22485 Msa1379140:CDS
AGAGGGGTAAAGATATATTC+GGG 0.529796 tig0020277:+22883 Msa1379140:three_prime_UTR
AACATCTTTACCTTCTGAGC+AGG 0.533206 tig0020277:+20882 Msa1379140:five_prime_UTR
AATAATCTATCTATTTAGGC+TGG 0.537486 tig0020277:+23021 Msa1379140:three_prime_UTR
GATGCCTTAAGTGGGCGGAC+TGG 0.545894 tig0020277:+22166 Msa1379140:CDS
TTTCCTGAAAATTAGATGGA+GGG 0.546268 tig0020277:-20390 None:intergenic
TAAAACACATCAACAAAGAT+GGG 0.548679 tig0020277:+22000 Msa1379140:CDS
AAGAGTAACCTGAGCACAGA+AGG 0.550563 tig0020277:-22666 None:intergenic
TTTATGCTGTTAATTGGAAG+GGG 0.566082 tig0020277:+21518 Msa1379140:CDS
AAAACTATTCAGGTGTACTC+GGG 0.566673 tig0020277:+23073 Msa1379140:three_prime_UTR
TGAGAGTTCCAAAACTCTCT+CGG 0.572947 tig0020277:-21582 None:intergenic
GCCGCGAGAGCACTTCTGGC+CGG 0.573681 tig0020277:-20746 None:intergenic
ATTAACAGCATAAAATGACT+TGG 0.574271 tig0020277:-21509 None:intergenic
TGAAAGAAACAACCGGTCCA+TGG 0.575404 tig0020277:+22492 Msa1379140:CDS
TAGAAAGAGTCTCTTTGGCA+CGG 0.583734 tig0020277:-22210 None:intergenic
GCGCTATCTCTTCATGCAGC+CGG 0.588002 tig0020277:+20727 Msa1379140:five_prime_UTR
ACGTTTAACTCTGCCTGCGA+CGG 0.593774 tig0020277:+20705 Msa1379140:five_prime_UTR
GTAGAATGTGCTCTTTGTGG+TGG 0.598700 tig0020277:+22596 Msa1379140:CDS
TCCAAAACTCTCTCGGCACG+CGG 0.613662 tig0020277:-21575 None:intergenic
GCCGGCCAGAAGTGCTCTCG+CGG 0.619458 tig0020277:+20745 Msa1379140:five_prime_UTR
TGAAGTTGGATGCCTTAAGT+GGG 0.620620 tig0020277:+22158 Msa1379140:CDS
TAATTCACAGTACCATGGAC+CGG 0.621024 tig0020277:-22504 None:intergenic
AGTTGGATGCCTTAAGTGGG+CGG 0.629952 tig0020277:+22161 Msa1379140:CDS
ACATCAACAAAGATGGGATG+CGG 0.631395 tig0020277:+22006 Msa1379140:CDS
TCTATCTCACCACTTTCACA+TGG 0.645943 tig0020277:+20356 Msa1379140:five_prime_UTR
ATATCTGCTTGATCTTAGAG+AGG 0.651787 tig0020277:+22042 Msa1379140:CDS
GAAAGAGTCTCTTTGGCACG+GGG 0.656351 tig0020277:-22208 None:intergenic
TCACATAATTCACAGTACCA+TGG 0.665195 tig0020277:-22509 None:intergenic
TCTGTTGGAGATATATAGAG+GGG 0.666100 tig0020277:+22867 Msa1379140:three_prime_UTR
GATCTTAGAGAGGCAACAGG+AGG 0.685846 tig0020277:+22052 Msa1379140:CDS
CAAAATACTGAAAAGCAGAG+TGG 0.687038 tig0020277:-20790 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTTCTTTCAAATTTA+TGG + tig0020277:21316-21335 Msa1379140:intron 10.0%
!!! TCATTATACTATTTTTTTTT+TGG - tig0020277:23124-23143 None:intergenic 10.0%
!!! TATTTTATGTCTTTCAATTT+GGG - tig0020277:20418-20437 None:intergenic 15.0%
!!! TTATTTTATGTCTTTCAATT+TGG - tig0020277:20419-20438 None:intergenic 15.0%
!!! AGAAATTTTAGGTTTTAATT+TGG - tig0020277:20457-20476 None:intergenic 15.0%
!!! AATCTTATCTTAGAAATTTT+AGG - tig0020277:20468-20487 None:intergenic 15.0%
!!! TATTGTTGTGATTTTTTAAT+AGG + tig0020277:20819-20838 Msa1379140:five_prime_UTR 15.0%
!!! ATCATATTTTTACTGTTATA+CGG - tig0020277:22124-22143 None:intergenic 15.0%
!!! AAAGTTTTGGATTTTGATTT+GGG - tig0020277:20534-20553 None:intergenic 20.0%
!! CAATACAAAATTAACCAAAA+AGG - tig0020277:20920-20939 None:intergenic 20.0%
!!! TAATTTTGTATCGATGAAAT+TGG + tig0020277:21037-21056 Msa1379140:intron 20.0%
!! AATCAAATATCAAACCAAAA+TGG - tig0020277:21080-21099 None:intergenic 20.0%
!!! CAAATTTATGGATTGAATTT+TGG + tig0020277:21328-21347 Msa1379140:intron 20.0%
!! TGAATTATAATTTATGCTCT+TGG + tig0020277:21352-21371 Msa1379140:intron 20.0%
!! TATATATTCCAAACATAACT+TGG + tig0020277:21718-21737 Msa1379140:intron 20.0%
!!! TGTAAGTTTAAAATTGAACA+TGG - tig0020277:21899-21918 None:intergenic 20.0%
!!! TTTTCTAATTTCAGAAAACT+TGG + tig0020277:21940-21959 Msa1379140:intron 20.0%
!!! TTTTCAAAATTCATTGAAGT+TGG + tig0020277:22144-22163 Msa1379140:CDS 20.0%
!! AAGAAGCAAGTATAAAAAAT+AGG - tig0020277:22748-22767 None:intergenic 20.0%
!!! TTTTGTTTCTATGAACTTAT+AGG + tig0020277:22843-22862 Msa1379140:intron 20.0%
!! TGACAATAATCTATCTATTT+AGG + tig0020277:23017-23036 Msa1379140:three_prime_UTR 20.0%
!! ATATTAAAAAGCCAAATACT+AGG + tig0020277:23201-23220 Msa1379140:three_prime_UTR 20.0%
!!! AAGTTTTGGATTTTGATTTG+GGG - tig0020277:20533-20552 None:intergenic 25.0%
!!! GAAAGTTTTGGATTTTGATT+TGG - tig0020277:20535-20554 None:intergenic 25.0%
!!! TGTTGTGATTTTTTAATAGG+TGG + tig0020277:20822-20841 Msa1379140:five_prime_UTR 25.0%
!!! GTTGTGATTTTTTAATAGGT+GGG + tig0020277:20823-20842 Msa1379140:five_prime_UTR 25.0%
!! TTTAGAGAGAAAATCCATTT+TGG + tig0020277:21063-21082 Msa1379140:intron 25.0%
! TAATCACTATGAAACTACAA+AGG - tig0020277:21132-21151 None:intergenic 25.0%
!!! TTCAGTATTTTGCTTTTGAA+TGG + tig0020277:21281-21300 Msa1379140:intron 25.0%
!!! AGCTTTTTTGTTGTGATTTT+CGG + tig0020277:21461-21480 Msa1379140:CDS 25.0%
! ATTAACAGCATAAAATGACT+TGG - tig0020277:21512-21531 None:intergenic 25.0%
!!! AGTCATTTTATGCTGTTAAT+TGG + tig0020277:21512-21531 Msa1379140:CDS 25.0%
!! ATTTTATGCTGTTAATTGGA+AGG + tig0020277:21516-21535 Msa1379140:CDS 25.0%
!! TTTTATGCTGTTAATTGGAA+GGG + tig0020277:21517-21536 Msa1379140:CDS 25.0%
!! TCTTCTAATTAATCTGAAGT+TGG + tig0020277:21853-21872 Msa1379140:intron 25.0%
! ATAAAACACATCAACAAAGA+TGG + tig0020277:21999-22018 Msa1379140:CDS 25.0%
! TAAAACACATCAACAAAGAT+GGG + tig0020277:22000-22019 Msa1379140:CDS 25.0%
! AGAGAAATAAATGCATGAAA+TGG - tig0020277:22406-22425 None:intergenic 25.0%
! AACCATGAAAAACTAATAAG+GGG - tig0020277:22437-22456 None:intergenic 25.0%
! CAACCATGAAAAACTAATAA+GGG - tig0020277:22438-22457 None:intergenic 25.0%
! ACAACCATGAAAAACTAATA+AGG - tig0020277:22439-22458 None:intergenic 25.0%
!!! TATTAGTTTTTCATGGTTGT+AGG + tig0020277:22439-22458 Msa1379140:intron 25.0%
! TATAAAAAATAGGCGAAGTT+AGG - tig0020277:22738-22757 None:intergenic 25.0%
! TCATCATCATCATTATTGAA+TGG + tig0020277:22806-22825 Msa1379140:intron 25.0%
! TCTGTTGAAGATATATAAGA+TGG + tig0020277:22907-22926 Msa1379140:three_prime_UTR 25.0%
! AATAATCTATCTATTTAGGC+TGG + tig0020277:23021-23040 Msa1379140:three_prime_UTR 25.0%
! CCTTTCACAAATTCAAAAAT+GGG - tig0020277:23176-23195 None:intergenic 25.0%
! ACCTTTCACAAATTCAAAAA+TGG - tig0020277:23177-23196 None:intergenic 25.0%
! AATTAGATGGAGGGAAATTT+TGG - tig0020277:20384-20403 None:intergenic 30.0%
TTTCCTGAAAATTAGATGGA+GGG - tig0020277:20393-20412 None:intergenic 30.0%
! TTTTCCTGAAAATTAGATGG+AGG - tig0020277:20394-20413 None:intergenic 30.0%
! GGATTTTCCTGAAAATTAGA+TGG - tig0020277:20397-20416 None:intergenic 30.0%
!!! GGTTTTAATTTGGAAACAGA+TGG - tig0020277:20447-20466 None:intergenic 30.0%
ATCGAAGGAGATAAAACAAA+TGG - tig0020277:20492-20511 None:intergenic 30.0%
! AAAGAGAAGAGAGAAAGTTT+TGG - tig0020277:20547-20566 None:intergenic 30.0%
CAGAGTTAAACGTAATTTCA+AGG - tig0020277:20698-20717 None:intergenic 30.0%
!!! ACTGGTTTGTATCTCTTTTA+TGG + tig0020277:21172-21191 Msa1379140:intron 30.0%
GCATGCAAAACAATCAAAAT+GGG - tig0020277:21227-21246 None:intergenic 30.0%
AGCATGCAAAACAATCAAAA+TGG - tig0020277:21228-21247 None:intergenic 30.0%
!!! ATTTTGATTGTTTTGCATGC+TGG + tig0020277:21227-21246 Msa1379140:intron 30.0%
! CCATCAAACTATCTAAAACA+AGG - tig0020277:21388-21407 None:intergenic 30.0%
!!! CCTTGTTTTAGATAGTTTGA+TGG + tig0020277:21385-21404 Msa1379140:intron 30.0%
TTTATGCTGTTAATTGGAAG+GGG + tig0020277:21518-21537 Msa1379140:CDS 30.0%
AAGTTGGCATTATCAATTGT+TGG + tig0020277:21869-21888 Msa1379140:intron 30.0%
AGTTGGCATTATCAATTGTT+GGG + tig0020277:21870-21889 Msa1379140:intron 30.0%
! ATGTGTTTTATCAATCTCCT+GGG - tig0020277:21990-22009 None:intergenic 30.0%
GAAATTGCAAAAGAAGTAAG+TGG - tig0020277:22084-22103 None:intergenic 30.0%
AGCTTCGTCATAGAAATATT+CGG + tig0020277:22249-22268 Msa1379140:intron 30.0%
TCCTTAGAAATATCTGATCT+TGG - tig0020277:22286-22305 None:intergenic 30.0%
!!! TTCCCCTTATTAGTTTTTCA+TGG + tig0020277:22432-22451 Msa1379140:intron 30.0%
GCACATTCTACAACAAAATA+TGG - tig0020277:22587-22606 None:intergenic 30.0%
TATGAACTTATAGGTTCTGT+TGG + tig0020277:22852-22871 Msa1379140:intron 30.0%
TTCTGTTGGAGATATATAGA+GGG + tig0020277:22866-22885 Msa1379140:three_prime_UTR 30.0%
TAGAGGGGTAAAGATATATT+CGG + tig0020277:22882-22901 Msa1379140:three_prime_UTR 30.0%
ACCTCTTCAAAAAACTATTC+AGG + tig0020277:23063-23082 Msa1379140:three_prime_UTR 30.0%
!!! ACCTGAATAGTTTTTTGAAG+AGG - tig0020277:23067-23086 None:intergenic 30.0%
AAAAACTATTCAGGTGTACT+CGG + tig0020277:23072-23091 Msa1379140:three_prime_UTR 30.0%
!! CCCATTTTTGAATTTGTGAA+AGG + tig0020277:23173-23192 Msa1379140:three_prime_UTR 30.0%
AACCAAATTTGCCTAGTATT+TGG - tig0020277:23215-23234 None:intergenic 30.0%
TGAGATAGAGAGATAGAATG+AGG - tig0020277:20345-20364 None:intergenic 35.0%
! TTTCCCTCCATCTAATTTTC+AGG + tig0020277:20387-20406 Msa1379140:five_prime_UTR 35.0%
ACAGAAACGTGAAAAAGAGA+AGG - tig0020277:20587-20606 None:intergenic 35.0%
CAAAATACTGAAAAGCAGAG+TGG - tig0020277:20793-20812 None:intergenic 35.0%
! GGTGAAAGACAAAACCTTTT+TGG + tig0020277:20903-20922 Msa1379140:intron 35.0%
ACAAAGGTTCGATTACGAAA+TGG - tig0020277:21116-21135 None:intergenic 35.0%
! GCTTTTGAATGGTTCATGTT+TGG + tig0020277:21292-21311 Msa1379140:intron 35.0%
! TTGTTGTGATTTTCGGAACA+TGG + tig0020277:21468-21487 Msa1379140:CDS 35.0%
ATCACACTCCAAGTTATGTT+TGG - tig0020277:21729-21748 None:intergenic 35.0%
! TGTGTTTTATCAATCTCCTG+GGG - tig0020277:21989-22008 None:intergenic 35.0%
! GATGTGTTTTATCAATCTCC+TGG - tig0020277:21991-22010 None:intergenic 35.0%
ATATCTGCTTGATCTTAGAG+AGG + tig0020277:22042-22061 Msa1379140:CDS 35.0%
! AACTCTAGAAAGAGTCTCTT+TGG - tig0020277:22218-22237 None:intergenic 35.0%
! GCCAAGATCAGATATTTCTA+AGG + tig0020277:22282-22301 Msa1379140:intron 35.0%
TAAATGCATGAAATGGACCA+GGG - tig0020277:22399-22418 None:intergenic 35.0%
ATAAATGCATGAAATGGACC+AGG - tig0020277:22400-22419 None:intergenic 35.0%
! TTTTTCATGGTTGTAGGTTG+CGG + tig0020277:22445-22464 Msa1379140:intron 35.0%
TCACATAATTCACAGTACCA+TGG - tig0020277:22512-22531 None:intergenic 35.0%
ATCTATTGTCCAGAAGTTCT+GGG + tig0020277:22534-22553 Msa1379140:CDS 35.0%
GCCATCTGCCATAAATAATT+GGG + tig0020277:22556-22575 Msa1379140:CDS 35.0%
TCCCAATTATTTATGGCAGA+TGG - tig0020277:22560-22579 None:intergenic 35.0%
TGGCTTCTCCCAATTATTTA+TGG - tig0020277:22567-22586 None:intergenic 35.0%
GTTCTGTTGGAGATATATAG+AGG + tig0020277:22865-22884 Msa1379140:three_prime_UTR 35.0%
TCTGTTGGAGATATATAGAG+GGG + tig0020277:22867-22886 Msa1379140:three_prime_UTR 35.0%
AGAGGGGTAAAGATATATTC+GGG + tig0020277:22883-22902 Msa1379140:three_prime_UTR 35.0%
AAAACTATTCAGGTGTACTC+GGG + tig0020277:23073-23092 Msa1379140:three_prime_UTR 35.0%
AGCCAAATACTAGGCAAATT+TGG + tig0020277:23210-23229 Msa1379140:three_prime_UTR 35.0%
TCTATCTCACCACTTTCACA+TGG + tig0020277:20356-20375 Msa1379140:five_prime_UTR 40.0%
!!! ATTTTGGAGCCATGTGAAAG+TGG - tig0020277:20368-20387 None:intergenic 40.0%
TTGGGGAAAAAGAGAGAGAA+GGG - tig0020277:20516-20535 None:intergenic 40.0%
TTTGGGGAAAAAGAGAGAGA+AGG - tig0020277:20517-20536 None:intergenic 40.0%
CTGCTACAAAGTCTCTTTCA+CGG + tig0020277:20647-20666 Msa1379140:five_prime_UTR 40.0%
AACATCTTTACCTTCTGAGC+AGG + tig0020277:20882-20901 Msa1379140:five_prime_UTR 40.0%
AAGGTTCGATTACGAAATGG+AGG - tig0020277:21113-21132 None:intergenic 40.0%
CCAGTTCAGTGAGTAGAAAA+GGG - tig0020277:21157-21176 None:intergenic 40.0%
ACCAGTTCAGTGAGTAGAAA+AGG - tig0020277:21158-21177 None:intergenic 40.0%
TGAGAGTTCCAAAACTCTCT+CGG - tig0020277:21585-21604 None:intergenic 40.0%
!!! GAGAGTTTTGGAACTCTCAT+TGG + tig0020277:21586-21605 Msa1379140:CDS 40.0%
!!! AGTTTTGGAACTCTCATTGG+AGG + tig0020277:21589-21608 Msa1379140:CDS 40.0%
CAGGAAGAAGTTGCAAAAAG+AGG + tig0020277:21776-21795 Msa1379140:intron 40.0%
ACATCAACAAAGATGGGATG+CGG + tig0020277:22006-22025 Msa1379140:CDS 40.0%
TTGAAGTTGGATGCCTTAAG+TGG + tig0020277:22157-22176 Msa1379140:CDS 40.0%
TGAAGTTGGATGCCTTAAGT+GGG + tig0020277:22158-22177 Msa1379140:CDS 40.0%
TAGAAAGAGTCTCTTTGGCA+CGG - tig0020277:22213-22232 None:intergenic 40.0%
AATCCTATCCTACTATGCTC+TGG + tig0020277:22352-22371 Msa1379140:CDS 40.0%
CGTAGTGTGAAAGAAACAAC+CGG + tig0020277:22485-22504 Msa1379140:CDS 40.0%
TAATTCACAGTACCATGGAC+CGG - tig0020277:22507-22526 None:intergenic 40.0%
GATCTATTGTCCAGAAGTTC+TGG + tig0020277:22533-22552 Msa1379140:CDS 40.0%
GGCCATCTGCCATAAATAAT+TGG + tig0020277:22555-22574 Msa1379140:CDS 40.0%
GTTGTAGAATGTGCTCTTTG+TGG + tig0020277:22593-22612 Msa1379140:CDS 40.0%
!! GCTTTTAGGTAGTCATCTGA+TGG + tig0020277:22629-22648 Msa1379140:exon 40.0%
GTAGTCATCTGATGGTCAAT+GGG + tig0020277:22637-22656 Msa1379140:exon 40.0%
TGGGGAAAAAGAGAGAGAAG+GGG - tig0020277:20515-20534 None:intergenic 45.0%
CTGCTTTGCGTGTGTGTATT+TGG - tig0020277:20617-20636 None:intergenic 45.0%
TTGTCTTTCACCTGCTCAGA+AGG - tig0020277:20895-20914 None:intergenic 45.0%
! CCCTTTTCTACTCACTGAAC+TGG + tig0020277:21154-21173 Msa1379140:intron 45.0%
! TTTTCAGCATAGACTGCTGC+AGG + tig0020277:21757-21776 Msa1379140:intron 45.0%
CTTGATCTTAGAGAGGCAAC+AGG + tig0020277:22049-22068 Msa1379140:CDS 45.0%
AGAAAGAGTCTCTTTGGCAC+GGG - tig0020277:22212-22231 None:intergenic 45.0%
CAACCAGAGCATAGTAGGAT+AGG - tig0020277:22358-22377 None:intergenic 45.0%
! TGCTCTGGTTGCAAGGTTTT+AGG + tig0020277:22367-22386 Msa1379140:intron 45.0%
!!! AAGGTTTTAGGACTCATCCC+TGG + tig0020277:22379-22398 Msa1379140:intron 45.0%
TGAAAGAAACAACCGGTCCA+TGG + tig0020277:22492-22511 Msa1379140:CDS 45.0%
GTAGAATGTGCTCTTTGTGG+TGG + tig0020277:22596-22615 Msa1379140:CDS 45.0%
! GTGGTACTACTGGTGCTTTT+AGG + tig0020277:22615-22634 Msa1379140:CDS 45.0%
GGTAGTCATCTGATGGTCAA+TGG + tig0020277:22636-22655 Msa1379140:exon 45.0%
AAGAGTAACCTGAGCACAGA+AGG - tig0020277:22669-22688 None:intergenic 45.0%
! CTATCTATTTAGGCTGGCTG+TGG + tig0020277:23027-23046 Msa1379140:three_prime_UTR 45.0%
AAGAGAGAGAAGGGGATCGA+AGG - tig0020277:20507-20526 None:intergenic 50.0%
ACGTTTAACTCTGCCTGCGA+CGG + tig0020277:20705-20724 Msa1379140:five_prime_UTR 50.0%
CTGTTAATTGGAAGGGGCAG+TGG + tig0020277:21524-21543 Msa1379140:CDS 50.0%
TTGGAGGAAGCTCGTCAACT+TGG + tig0020277:21605-21624 Msa1379140:CDS 50.0%
AATCTCCTGGGGCAAACTCT+TGG - tig0020277:21978-21997 None:intergenic 50.0%
GATCTTAGAGAGGCAACAGG+AGG + tig0020277:22052-22071 Msa1379140:CDS 50.0%
AGTTGGATGCCTTAAGTGGG+CGG + tig0020277:22161-22180 Msa1379140:CDS 50.0%
GAAAGAGTCTCTTTGGCACG+GGG - tig0020277:22211-22230 None:intergenic 50.0%
CCTTGCAACCAGAGCATAGT+AGG - tig0020277:22363-22382 None:intergenic 50.0%
CCTACTATGCTCTGGTTGCA+AGG + tig0020277:22360-22379 Msa1379140:CDS 50.0%
GCTCTTTGTGGTGGTACTAC+TGG + tig0020277:22605-22624 Msa1379140:CDS 50.0%
! TCTATTTAGGCTGGCTGTGG+AGG + tig0020277:23030-23049 Msa1379140:three_prime_UTR 50.0%
GCGCTATCTCTTCATGCAGC+CGG + tig0020277:20727-20746 Msa1379140:five_prime_UTR 55.0%
TCCAAAACTCTCTCGGCACG+CGG - tig0020277:21578-21597 None:intergenic 55.0%
TGTTGCCAAGAGTTTGCCCC+AGG + tig0020277:21970-21989 Msa1379140:CDS 55.0%
GGTTCATGCCTTCTGTGCTC+AGG + tig0020277:22658-22677 Msa1379140:three_prime_UTR 55.0%
TGAAGAGATAGCGCCGTCGC+AGG - tig0020277:20721-20740 None:intergenic 60.0%
! TCCGCGTGCCGAGAGAGTTT+TGG + tig0020277:21574-21593 Msa1379140:CDS 60.0%
GATGCCTTAAGTGGGCGGAC+TGG + tig0020277:22166-22185 Msa1379140:CDS 60.0%
TGGACCAGTCCGCCCACTTA+AGG - tig0020277:22173-22192 None:intergenic 60.0%
!! CGGGGCATTGGCTGAGAACA+TGG - tig0020277:22193-22212 None:intergenic 60.0%
GTCTCTTTGGCACGGGGCAT+TGG - tig0020277:22205-22224 None:intergenic 60.0%
GGCAGATGGCCCAGAACTTC+TGG - tig0020277:22546-22565 None:intergenic 60.0%
!! CATCGCCGCGAGAGCACTTC+TGG - tig0020277:20753-20772 None:intergenic 65.0%
!! CAGAAGTGCTCTCGCGGCGA+TGG + tig0020277:20751-20770 Msa1379140:five_prime_UTR 65.0%
!! GCCGGCCAGAAGTGCTCTCG+CGG + tig0020277:20745-20764 Msa1379140:five_prime_UTR 70.0%
!! GCCGCGAGAGCACTTCTGGC+CGG - tig0020277:20749-20768 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
tig0020277 gene 20339 23263 20339 ID=Msa1379140;Name=Msa1379140
tig0020277 mRNA 20339 23263 20339 ID=Msa1379140-mRNA-1;Parent=Msa1379140;Name=Msa1379140-mRNA-1;_AED=0.17;_eAED=0.22;_QI=569|0.4|0.5|1|0.6|0.5|6|438|250
tig0020277 exon 20339 20903 20339 ID=Msa1379140-mRNA-1:exon:6430;Parent=Msa1379140-mRNA-1
tig0020277 exon 21400 21626 21400 ID=Msa1379140-mRNA-1:exon:6431;Parent=Msa1379140-mRNA-1
tig0020277 exon 21954 22254 21954 ID=Msa1379140-mRNA-1:exon:6432;Parent=Msa1379140-mRNA-1
tig0020277 exon 22333 22381 22333 ID=Msa1379140-mRNA-1:exon:6433;Parent=Msa1379140-mRNA-1
tig0020277 exon 22461 22679 22461 ID=Msa1379140-mRNA-1:exon:6434;Parent=Msa1379140-mRNA-1
tig0020277 exon 22865 23263 22865 ID=Msa1379140-mRNA-1:exon:6435;Parent=Msa1379140-mRNA-1
tig0020277 five_prime_UTR 20339 20903 20339 ID=Msa1379140-mRNA-1:five_prime_utr;Parent=Msa1379140-mRNA-1
tig0020277 five_prime_UTR 21400 21403 21400 ID=Msa1379140-mRNA-1:five_prime_utr;Parent=Msa1379140-mRNA-1
tig0020277 CDS 21404 21626 21404 ID=Msa1379140-mRNA-1:cds;Parent=Msa1379140-mRNA-1
tig0020277 CDS 21954 22254 21954 ID=Msa1379140-mRNA-1:cds;Parent=Msa1379140-mRNA-1
tig0020277 CDS 22333 22381 22333 ID=Msa1379140-mRNA-1:cds;Parent=Msa1379140-mRNA-1
tig0020277 CDS 22461 22640 22461 ID=Msa1379140-mRNA-1:cds;Parent=Msa1379140-mRNA-1
tig0020277 three_prime_UTR 22641 22679 22641 ID=Msa1379140-mRNA-1:three_prime_utr;Parent=Msa1379140-mRNA-1
tig0020277 three_prime_UTR 22865 23263 22865 ID=Msa1379140-mRNA-1:three_prime_utr;Parent=Msa1379140-mRNA-1
Gene Sequence

>Msa1379140

ATGGCTTCTTTTGTTATGATATCAAATCTAAATGTATCTGTTATGATAGTTTTTGAGAGCTTTTTTGTTGTGATTTTCGGAACATGGTGTTCATGCTTAGCTTATCCAAGTCATTTTATGCTGTTAATTGGAAGGGGCAGTGGTTTTGTCGCTGTTGAGATTCTGTTTACTCCGCGTGCCGAGAGAGTTTTGGAACTCTCATTGGAGGAAGCTCGTCAACTTGAAAACTTGGTCTACAGTGTTGCCAAGAGTTTGCCCCAGGAGATTGATAAAACACATCAACAAAGATGGGATGCGGTGATTGCTAGTCAATATCTGCTTGATCTTAGAGAGGCAACAGGAGGTCGTATCCACTTACTTCTTTTGCAATTTCTTTTAACAGTTTGCATACCGTATAACAGTAAAAATATGATTTTTCAAAATTCATTGAAGTTGGATGCCTTAAGTGGGCGGACTGGTCCATGTTCTCAGCCAATGCCCCGTGCCAAAGAGACTCTTTCTAGAGTTGCTGTCACAAGAGCTTCACGATTTGAGAATGTGTTAAATCCTATCCTACTATGCTCTGGTTGCAAGGTTGCGGTGCATTCAGTTTGCTATCGTAGTGTGAAAGAAACAACCGGTCCATGGTACTGTGAATTATGTGAAGATCTATTGTCCAGAAGTTCTGGGCCATCTGCCATAAATAATTGGGAGAAGCCATATTTTGTTGTAGAATGTGCTCTTTGTGGTGGTACTACTGGTGCTTTTAGGTAG
Protein sequence

>Msa1379140

MASFVMISNLNVSVMIVFESFFVVIFGTWCSCLAYPSHFMLLIGRGSGFVAVEILFTPRAERVLELSLEEARQLENLVYSVAKSLPQEIDKTHQQRWDAVIASQYLLDLREATGGRIHLLLLQFLLTVCIPYNSKNMIFQNSLKLDALSGRTGPCSQPMPRAKETLSRVAVTRASRFENVLNPILLCSGCKVAVHSVCYRSVKETTGPWYCELCEDLLSRSSGPSAINNWEKPYFVVECALCGGTTGAFR*