AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0806690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0806690 MtrunA17_Chr3g0115211 63.793 232 41 3 62 250 892 1123 7.02e-86 278
Msa0806690 MtrunA17_Chr8g0335041 58.763 97 12 2 137 207 1 95 7.92e-26 97.4
Msa0806690 MtrunA17_Chr3g0131711 90.909 33 3 0 42 74 156 188 1.64e-11 63.9
Msa0806690 MtrunA17_Chr8g0385811 90.909 33 3 0 42 74 160 192 1.64e-11 63.9
Msa0806690 MtrunA17_Chr3g0134631 90.909 33 3 0 42 74 161 193 1.64e-11 63.9
Msa0806690 MtrunA17_Chr3g0135231 90.909 33 3 0 42 74 147 179 4.74e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0806690 AT1G77800.3 38.182 220 88 8 72 250 875 1087 3.63e-30 119
Msa0806690 AT1G77800.4 38.182 220 88 8 72 250 875 1087 3.91e-30 119
Msa0806690 AT1G77800.1 38.182 220 88 8 72 250 825 1037 4.24e-30 119
Msa0806690 AT1G77800.2 38.182 220 88 8 72 250 825 1037 4.32e-30 119
Msa0806690 AT1G77800.5 38.182 220 88 8 72 250 875 1087 4.40e-30 118
Msa0806690 AT1G77800.6 38.182 220 88 8 72 250 848 1060 4.79e-30 118
Msa0806690 AT1G77800.7 38.182 220 88 8 72 250 806 1018 5.29e-30 118

Find 84 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCAAATTTGCCTAGTATT+TGG 0.151147 5_3:-26477440 None:intergenic
AATTAGATGGAGGGAAATTT+TGG 0.174206 5_3:-26474612 None:intergenic
TGACAATAATCTATCTATTT+AGG 0.192693 5_3:+26477245 Msa0806690:three_prime_UTR
GGCCATCTGCCATAAATAAT+TGG 0.197244 5_3:+26476786 Msa0806690:CDS
TGGCTTCTCCCAATTATTTA+TGG 0.197593 5_3:-26476795 None:intergenic
AGCCAAATACTAGGCAAATT+TGG 0.206453 5_3:+26477438 Msa0806690:three_prime_UTR
CTGCTTTGCGTGTGTGTATT+TGG 0.249599 5_3:-26474845 None:intergenic
GCACAAAATCTGCTAGTATT+TGG 0.260441 5_3:-26477471 None:intergenic
GGCAGATGGCCCAGAACTTC+TGG 0.261026 5_3:-26476774 None:intergenic
AAAGAGAAGAGAGAAAGTTT+TGG 0.279987 5_3:-26474775 None:intergenic
GATCTATTGTCCAGAAGTTC+TGG 0.291520 5_3:+26476764 Msa0806690:CDS
TCCGCGTGCCGAGAGAGTTT+TGG 0.296686 5_3:+26475805 Msa0806690:CDS
CATCGCCGCGAGAGCACTTC+TGG 0.315808 5_3:-26474981 None:intergenic
GCACATTCTACAACAAAATA+TGG 0.320317 5_3:-26476815 None:intergenic
AACTCTAGAAAGAGTCTCTT+TGG 0.352202 5_3:-26476446 None:intergenic
TAGAGTGGTAAAGATATATT+CGG 0.365468 5_3:+26477110 Msa0806690:three_prime_UTR
GTCTCTTTGGCACGGGGCAT+TGG 0.373676 5_3:-26476433 None:intergenic
GCTCTTTGTGGTGGTACTAC+TGG 0.402180 5_3:+26476836 Msa0806690:CDS
TTGAAGTTGGATGCCTTAAG+TGG 0.416921 5_3:+26476388 Msa0806690:CDS
CCTTGCAACCAGAGCATAGT+AGG 0.420383 5_3:-26476591 None:intergenic
GGTAGTCATCTGATGGTCAA+TGG 0.424003 5_3:+26476867 Msa0806690:exon
CAGAGTTAAACGTAATTTCA+AGG 0.426357 5_3:-26474926 None:intergenic
CTGTTAATTGGAAGGGGCAG+TGG 0.432063 5_3:+26475755 Msa0806690:CDS
CTGCTACAAAGTCTCTTTCA+CGG 0.442513 5_3:+26474878 Msa0806690:five_prime_UTR
ATCGAAGGAGATAAAACAAA+TGG 0.448417 5_3:-26474720 None:intergenic
TATGAACTTATAGGTTCTGT+TGG 0.451456 5_3:+26477080 Msa0806690:intron
CGGGGCATTGGCTGAGAACA+TGG 0.461925 5_3:-26476421 None:intergenic
ATAGAGAGATAGAATGAGGA+TGG 0.467266 5_3:-26474569 None:intergenic
GGTTCATGCCTTCTGTGCTC+AGG 0.470096 5_3:+26476889 Msa0806690:three_prime_UTR
AATCTCCTGGGGCAAACTCT+TGG 0.470177 5_3:-26476206 None:intergenic
AAGAGAGAGAAGGGGATCGA+AGG 0.470935 5_3:-26474735 None:intergenic
GCCATCTGCCATAAATAATT+GGG 0.472144 5_3:+26476787 Msa0806690:CDS
TGGACCAGTCCGCCCACTTA+AGG 0.473529 5_3:-26476401 None:intergenic
TCCCAATTATTTATGGCAGA+TGG 0.474546 5_3:-26476788 None:intergenic
GAAATTGCAAAAGAAGTAAG+TGG 0.476640 5_3:-26476312 None:intergenic
ATAAAACACATCAACAAAGA+TGG 0.489693 5_3:+26476230 Msa0806690:CDS
CAGAAGTGCTCTCGCGGCGA+TGG 0.493289 5_3:+26474982 Msa0806690:five_prime_UTR
CCTACTATGCTCTGGTTGCA+AGG 0.501390 5_3:+26476591 Msa0806690:CDS
TCTATTTAGGCTGGCTGTGG+AGG 0.501898 5_3:+26477258 Msa0806690:three_prime_UTR
GTTGTAGAATGTGCTCTTTG+TGG 0.503308 5_3:+26476824 Msa0806690:CDS
GTAGTCATCTGATGGTCAAT+GGG 0.504537 5_3:+26476868 Msa0806690:exon
TTGTCTTTCACCTGCTCAGA+AGG 0.505332 5_3:-26475123 None:intergenic
ATCTATTGTCCAGAAGTTCT+GGG 0.505690 5_3:+26476765 Msa0806690:CDS
CAACCAGAGCATAGTAGGAT+AGG 0.511552 5_3:-26476586 None:intergenic
TGAAGAGATAGCGCCGTCGC+AGG 0.513649 5_3:-26474949 None:intergenic
TCTGTTGAAGATATATAAGA+TGG 0.514275 5_3:+26477135 Msa0806690:three_prime_UTR
TGTTGCCAAGAGTTTGCCCC+AGG 0.516719 5_3:+26476201 Msa0806690:CDS
CTTGATCTTAGAGAGGCAAC+AGG 0.517782 5_3:+26476280 Msa0806690:CDS
AGAAAGAGTCTCTTTGGCAC+GGG 0.519668 5_3:-26476440 None:intergenic
TTGGAGGAAGCTCGTCAACT+TGG 0.522024 5_3:+26475836 Msa0806690:CDS
CTATCTATTTAGGCTGGCTG+TGG 0.523471 5_3:+26477255 Msa0806690:three_prime_UTR
AATCCTATCCTACTATGCTC+TGG 0.524276 5_3:+26476583 Msa0806690:CDS
TGAGATAGAGAGATAGAATG+AGG 0.524760 5_3:-26474573 None:intergenic
CGTAGTGTGAAAGAAACAAC+CGG 0.529590 5_3:+26476716 Msa0806690:CDS
AACATCTTTACCTTCTGAGC+AGG 0.533206 5_3:+26475113 Msa0806690:five_prime_UTR
AATAATCTATCTATTTAGGC+TGG 0.537486 5_3:+26477249 Msa0806690:three_prime_UTR
AGAGTGGTAAAGATATATTC+GGG 0.544801 5_3:+26477111 Msa0806690:three_prime_UTR
GATGCCTTAAGTGGGCGGAC+TGG 0.545894 5_3:+26476397 Msa0806690:CDS
TTTCCTGAAAATTAGATGGA+GGG 0.546268 5_3:-26474621 None:intergenic
TAAAACACATCAACAAAGAT+GGG 0.548679 5_3:+26476231 Msa0806690:CDS
AAGAGTAACCTGAGCACAGA+AGG 0.550563 5_3:-26476897 None:intergenic
TTTATGCTGTTAATTGGAAG+GGG 0.566082 5_3:+26475749 Msa0806690:CDS
AAAACTATTCAGGTGTACTC+GGG 0.566673 5_3:+26477301 Msa0806690:three_prime_UTR
TGAGAGTTCCAAAACTCTCT+CGG 0.572947 5_3:-26475813 None:intergenic
GCCGCGAGAGCACTTCTGGC+CGG 0.573681 5_3:-26474977 None:intergenic
ATTAACAGCATAAAATGACT+TGG 0.574271 5_3:-26475740 None:intergenic
TGAAAGAAACAACCGGTCCA+TGG 0.575404 5_3:+26476723 Msa0806690:CDS
TAGAAAGAGTCTCTTTGGCA+CGG 0.583734 5_3:-26476441 None:intergenic
GCGCTATCTCTTCATGCAGC+CGG 0.588002 5_3:+26474958 Msa0806690:five_prime_UTR
ACGTTTAACTCTGCCTGCGA+CGG 0.593774 5_3:+26474936 Msa0806690:five_prime_UTR
GTAGAATGTGCTCTTTGTGG+TGG 0.598700 5_3:+26476827 Msa0806690:CDS
TCCAAAACTCTCTCGGCACG+CGG 0.613662 5_3:-26475806 None:intergenic
GCCGGCCAGAAGTGCTCTCG+CGG 0.619458 5_3:+26474976 Msa0806690:five_prime_UTR
TGAAGTTGGATGCCTTAAGT+GGG 0.620620 5_3:+26476389 Msa0806690:CDS
TAATTCACAGTACCATGGAC+CGG 0.621024 5_3:-26476735 None:intergenic
TCTGTTGGAGATATATAGAG+TGG 0.623913 5_3:+26477095 Msa0806690:three_prime_UTR
AGTTGGATGCCTTAAGTGGG+CGG 0.629952 5_3:+26476392 Msa0806690:CDS
ACATCAACAAAGATGGGATG+CGG 0.631395 5_3:+26476237 Msa0806690:CDS
TCTATCTCACCACTTTCACA+TGG 0.645943 5_3:+26474587 Msa0806690:five_prime_UTR
ATATCTGCTTGATCTTAGAG+AGG 0.651787 5_3:+26476273 Msa0806690:CDS
GAAAGAGTCTCTTTGGCACG+GGG 0.656351 5_3:-26476439 None:intergenic
TCACATAATTCACAGTACCA+TGG 0.665195 5_3:-26476740 None:intergenic
GATCTTAGAGAGGCAACAGG+AGG 0.685846 5_3:+26476283 Msa0806690:CDS
CAAAATACTGAAAAGCAGAG+TGG 0.687038 5_3:-26475021 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTTCTTTCAAATTTA+TGG + chr5_3:26475547-26475566 Msa0806690:intron 10.0%
!!! TCATTATACTATTTTTTTTT+TGG - chr5_3:26477352-26477371 None:intergenic 10.0%
!!! TATTTTATGTCTTTCAATTT+GGG - chr5_3:26474649-26474668 None:intergenic 15.0%
!!! TTATTTTATGTCTTTCAATT+TGG - chr5_3:26474650-26474669 None:intergenic 15.0%
!!! AGAAATTTTAGGTTTTAATT+TGG - chr5_3:26474688-26474707 None:intergenic 15.0%
!!! AATCTTATCTTAGAAATTTT+AGG - chr5_3:26474699-26474718 None:intergenic 15.0%
!!! TATTGTTGTGATTTTTTAAT+AGG + chr5_3:26475050-26475069 Msa0806690:five_prime_UTR 15.0%
!!! ATCATATTTTTACTGTTATA+CGG - chr5_3:26476355-26476374 None:intergenic 15.0%
!!! AAAGTTTTGGATTTTGATTT+GGG - chr5_3:26474765-26474784 None:intergenic 20.0%
!! CAATACAAAATTAACCAAAA+AGG - chr5_3:26475151-26475170 None:intergenic 20.0%
!!! TAATTTTGTATCGATGAAAT+TGG + chr5_3:26475268-26475287 Msa0806690:intron 20.0%
!! AATCAAATATCAAACCAAAA+TGG - chr5_3:26475311-26475330 None:intergenic 20.0%
!!! CAAATTTATGGATTGAATTT+TGG + chr5_3:26475559-26475578 Msa0806690:intron 20.0%
!! TGAATTATAATTTATGCTCT+TGG + chr5_3:26475583-26475602 Msa0806690:intron 20.0%
!! TATATATTCCAAACATAACT+TGG + chr5_3:26475949-26475968 Msa0806690:intron 20.0%
!!! TGTAAGTTTAAAATTGAACA+TGG - chr5_3:26476130-26476149 None:intergenic 20.0%
!!! TTTTCTAATTTCAGAAAACT+TGG + chr5_3:26476171-26476190 Msa0806690:intron 20.0%
!!! TTTTCAAAATTCATTGAAGT+TGG + chr5_3:26476375-26476394 Msa0806690:CDS 20.0%
!! AAGAAGCAAGTATAAAAAAT+AGG - chr5_3:26476979-26476998 None:intergenic 20.0%
!!! TTTTGTTTCTATGAACTTAT+AGG + chr5_3:26477071-26477090 Msa0806690:intron 20.0%
!! TGACAATAATCTATCTATTT+AGG + chr5_3:26477245-26477264 Msa0806690:three_prime_UTR 20.0%
!! ATATTAAAAAGCCAAATACT+AGG + chr5_3:26477429-26477448 Msa0806690:three_prime_UTR 20.0%
!!! AAGTTTTGGATTTTGATTTG+GGG - chr5_3:26474764-26474783 None:intergenic 25.0%
!!! GAAAGTTTTGGATTTTGATT+TGG - chr5_3:26474766-26474785 None:intergenic 25.0%
!!! TGTTGTGATTTTTTAATAGG+TGG + chr5_3:26475053-26475072 Msa0806690:five_prime_UTR 25.0%
!!! GTTGTGATTTTTTAATAGGT+GGG + chr5_3:26475054-26475073 Msa0806690:five_prime_UTR 25.0%
!! TTTAGAGAGAAAATCCATTT+TGG + chr5_3:26475294-26475313 Msa0806690:intron 25.0%
! TAATCACTATGAAACTACAA+AGG - chr5_3:26475363-26475382 None:intergenic 25.0%
!!! TTCAGTATTTTGCTTTTGAA+TGG + chr5_3:26475512-26475531 Msa0806690:intron 25.0%
!!! AGCTTTTTTGTTGTGATTTT+CGG + chr5_3:26475692-26475711 Msa0806690:CDS 25.0%
! ATTAACAGCATAAAATGACT+TGG - chr5_3:26475743-26475762 None:intergenic 25.0%
!!! AGTCATTTTATGCTGTTAAT+TGG + chr5_3:26475743-26475762 Msa0806690:CDS 25.0%
!! ATTTTATGCTGTTAATTGGA+AGG + chr5_3:26475747-26475766 Msa0806690:CDS 25.0%
!! TTTTATGCTGTTAATTGGAA+GGG + chr5_3:26475748-26475767 Msa0806690:CDS 25.0%
!! TCTTCTAATTAATCTGAAGT+TGG + chr5_3:26476084-26476103 Msa0806690:intron 25.0%
! ATAAAACACATCAACAAAGA+TGG + chr5_3:26476230-26476249 Msa0806690:CDS 25.0%
! TAAAACACATCAACAAAGAT+GGG + chr5_3:26476231-26476250 Msa0806690:CDS 25.0%
! AGAGAAATAAATGCATGAAA+TGG - chr5_3:26476637-26476656 None:intergenic 25.0%
! AACCATGAAAAACTAATAAG+GGG - chr5_3:26476668-26476687 None:intergenic 25.0%
! CAACCATGAAAAACTAATAA+GGG - chr5_3:26476669-26476688 None:intergenic 25.0%
! ACAACCATGAAAAACTAATA+AGG - chr5_3:26476670-26476689 None:intergenic 25.0%
!!! TATTAGTTTTTCATGGTTGT+AGG + chr5_3:26476670-26476689 Msa0806690:intron 25.0%
! TATAAAAAATAGGCGAAGTT+AGG - chr5_3:26476969-26476988 None:intergenic 25.0%
! TCATCATCATCATTATTGAA+TGG + chr5_3:26477034-26477053 Msa0806690:intron 25.0%
! TAGAGTGGTAAAGATATATT+CGG + chr5_3:26477110-26477129 Msa0806690:three_prime_UTR 25.0%
! TCTGTTGAAGATATATAAGA+TGG + chr5_3:26477135-26477154 Msa0806690:three_prime_UTR 25.0%
! AATAATCTATCTATTTAGGC+TGG + chr5_3:26477249-26477268 Msa0806690:three_prime_UTR 25.0%
! CCTTTCACAAATTCAAAAAT+GGG - chr5_3:26477404-26477423 None:intergenic 25.0%
! ACCTTTCACAAATTCAAAAA+TGG - chr5_3:26477405-26477424 None:intergenic 25.0%
! AATTAGATGGAGGGAAATTT+TGG - chr5_3:26474615-26474634 None:intergenic 30.0%
TTTCCTGAAAATTAGATGGA+GGG - chr5_3:26474624-26474643 None:intergenic 30.0%
! TTTTCCTGAAAATTAGATGG+AGG - chr5_3:26474625-26474644 None:intergenic 30.0%
! GGATTTTCCTGAAAATTAGA+TGG - chr5_3:26474628-26474647 None:intergenic 30.0%
!!! GGTTTTAATTTGGAAACAGA+TGG - chr5_3:26474678-26474697 None:intergenic 30.0%
ATCGAAGGAGATAAAACAAA+TGG - chr5_3:26474723-26474742 None:intergenic 30.0%
! AAAGAGAAGAGAGAAAGTTT+TGG - chr5_3:26474778-26474797 None:intergenic 30.0%
CAGAGTTAAACGTAATTTCA+AGG - chr5_3:26474929-26474948 None:intergenic 30.0%
!!! ACTGGTTTGTATCTCTTTTA+TGG + chr5_3:26475403-26475422 Msa0806690:intron 30.0%
GCATGCAAAACAATCAAAAT+GGG - chr5_3:26475458-26475477 None:intergenic 30.0%
AGCATGCAAAACAATCAAAA+TGG - chr5_3:26475459-26475478 None:intergenic 30.0%
!!! ATTTTGATTGTTTTGCATGC+TGG + chr5_3:26475458-26475477 Msa0806690:intron 30.0%
! CCATCAAACTATCTAAAACA+AGG - chr5_3:26475619-26475638 None:intergenic 30.0%
!!! CCTTGTTTTAGATAGTTTGA+TGG + chr5_3:26475616-26475635 Msa0806690:intron 30.0%
TTTATGCTGTTAATTGGAAG+GGG + chr5_3:26475749-26475768 Msa0806690:CDS 30.0%
AAGTTGGCATTATCAATTGT+TGG + chr5_3:26476100-26476119 Msa0806690:intron 30.0%
AGTTGGCATTATCAATTGTT+GGG + chr5_3:26476101-26476120 Msa0806690:intron 30.0%
! ATGTGTTTTATCAATCTCCT+GGG - chr5_3:26476221-26476240 None:intergenic 30.0%
GAAATTGCAAAAGAAGTAAG+TGG - chr5_3:26476315-26476334 None:intergenic 30.0%
AGCTTCGTCATAGAAATATT+CGG + chr5_3:26476480-26476499 Msa0806690:intron 30.0%
TCCTTAGAAATATCTGATCT+TGG - chr5_3:26476517-26476536 None:intergenic 30.0%
!!! TTCCCCTTATTAGTTTTTCA+TGG + chr5_3:26476663-26476682 Msa0806690:intron 30.0%
GCACATTCTACAACAAAATA+TGG - chr5_3:26476818-26476837 None:intergenic 30.0%
TATGAACTTATAGGTTCTGT+TGG + chr5_3:26477080-26477099 Msa0806690:intron 30.0%
AGAGTGGTAAAGATATATTC+GGG + chr5_3:26477111-26477130 Msa0806690:three_prime_UTR 30.0%
ACCTCTTCAAAAAACTATTC+AGG + chr5_3:26477291-26477310 Msa0806690:three_prime_UTR 30.0%
!!! ACCTGAATAGTTTTTTGAAG+AGG - chr5_3:26477295-26477314 None:intergenic 30.0%
AAAAACTATTCAGGTGTACT+CGG + chr5_3:26477300-26477319 Msa0806690:three_prime_UTR 30.0%
!! CCCATTTTTGAATTTGTGAA+AGG + chr5_3:26477401-26477420 Msa0806690:three_prime_UTR 30.0%
AACCAAATTTGCCTAGTATT+TGG - chr5_3:26477443-26477462 None:intergenic 30.0%
TGAGATAGAGAGATAGAATG+AGG - chr5_3:26474576-26474595 None:intergenic 35.0%
! TTTCCCTCCATCTAATTTTC+AGG + chr5_3:26474618-26474637 Msa0806690:five_prime_UTR 35.0%
ACAGAAACGTGAAAAAGAGA+AGG - chr5_3:26474818-26474837 None:intergenic 35.0%
CAAAATACTGAAAAGCAGAG+TGG - chr5_3:26475024-26475043 None:intergenic 35.0%
! GGTGAAAGACAAAACCTTTT+TGG + chr5_3:26475134-26475153 Msa0806690:intron 35.0%
ACAAAGGTTCGATTACGAAA+TGG - chr5_3:26475347-26475366 None:intergenic 35.0%
! GCTTTTGAATGGTTCATGTT+TGG + chr5_3:26475523-26475542 Msa0806690:intron 35.0%
! TTGTTGTGATTTTCGGAACA+TGG + chr5_3:26475699-26475718 Msa0806690:CDS 35.0%
ATCACACTCCAAGTTATGTT+TGG - chr5_3:26475960-26475979 None:intergenic 35.0%
! TGTGTTTTATCAATCTCCTG+GGG - chr5_3:26476220-26476239 None:intergenic 35.0%
! GATGTGTTTTATCAATCTCC+TGG - chr5_3:26476222-26476241 None:intergenic 35.0%
ATATCTGCTTGATCTTAGAG+AGG + chr5_3:26476273-26476292 Msa0806690:CDS 35.0%
! AACTCTAGAAAGAGTCTCTT+TGG - chr5_3:26476449-26476468 None:intergenic 35.0%
! GCCAAGATCAGATATTTCTA+AGG + chr5_3:26476513-26476532 Msa0806690:intron 35.0%
TAAATGCATGAAATGGACCA+GGG - chr5_3:26476630-26476649 None:intergenic 35.0%
ATAAATGCATGAAATGGACC+AGG - chr5_3:26476631-26476650 None:intergenic 35.0%
! TTTTTCATGGTTGTAGGTTG+CGG + chr5_3:26476676-26476695 Msa0806690:intron 35.0%
TCACATAATTCACAGTACCA+TGG - chr5_3:26476743-26476762 None:intergenic 35.0%
ATCTATTGTCCAGAAGTTCT+GGG + chr5_3:26476765-26476784 Msa0806690:CDS 35.0%
GCCATCTGCCATAAATAATT+GGG + chr5_3:26476787-26476806 Msa0806690:CDS 35.0%
TCCCAATTATTTATGGCAGA+TGG - chr5_3:26476791-26476810 None:intergenic 35.0%
TGGCTTCTCCCAATTATTTA+TGG - chr5_3:26476798-26476817 None:intergenic 35.0%
TCTGTTGGAGATATATAGAG+TGG + chr5_3:26477095-26477114 Msa0806690:three_prime_UTR 35.0%
AAAACTATTCAGGTGTACTC+GGG + chr5_3:26477301-26477320 Msa0806690:three_prime_UTR 35.0%
AGCCAAATACTAGGCAAATT+TGG + chr5_3:26477438-26477457 Msa0806690:three_prime_UTR 35.0%
TCTATCTCACCACTTTCACA+TGG + chr5_3:26474587-26474606 Msa0806690:five_prime_UTR 40.0%
!!! ATTTTGGAGCCATGTGAAAG+TGG - chr5_3:26474599-26474618 None:intergenic 40.0%
TTGGGGAAAAAGAGAGAGAA+GGG - chr5_3:26474747-26474766 None:intergenic 40.0%
TTTGGGGAAAAAGAGAGAGA+AGG - chr5_3:26474748-26474767 None:intergenic 40.0%
CTGCTACAAAGTCTCTTTCA+CGG + chr5_3:26474878-26474897 Msa0806690:five_prime_UTR 40.0%
AACATCTTTACCTTCTGAGC+AGG + chr5_3:26475113-26475132 Msa0806690:five_prime_UTR 40.0%
AAGGTTCGATTACGAAATGG+AGG - chr5_3:26475344-26475363 None:intergenic 40.0%
CCAGTTCAGTGAGTAGAAAA+GGG - chr5_3:26475388-26475407 None:intergenic 40.0%
ACCAGTTCAGTGAGTAGAAA+AGG - chr5_3:26475389-26475408 None:intergenic 40.0%
TGAGAGTTCCAAAACTCTCT+CGG - chr5_3:26475816-26475835 None:intergenic 40.0%
!!! GAGAGTTTTGGAACTCTCAT+TGG + chr5_3:26475817-26475836 Msa0806690:CDS 40.0%
!!! AGTTTTGGAACTCTCATTGG+AGG + chr5_3:26475820-26475839 Msa0806690:CDS 40.0%
CAGGAAGAAGTTGCAAAAAG+AGG + chr5_3:26476007-26476026 Msa0806690:intron 40.0%
ACATCAACAAAGATGGGATG+CGG + chr5_3:26476237-26476256 Msa0806690:CDS 40.0%
TTGAAGTTGGATGCCTTAAG+TGG + chr5_3:26476388-26476407 Msa0806690:CDS 40.0%
TGAAGTTGGATGCCTTAAGT+GGG + chr5_3:26476389-26476408 Msa0806690:CDS 40.0%
TAGAAAGAGTCTCTTTGGCA+CGG - chr5_3:26476444-26476463 None:intergenic 40.0%
AATCCTATCCTACTATGCTC+TGG + chr5_3:26476583-26476602 Msa0806690:CDS 40.0%
CGTAGTGTGAAAGAAACAAC+CGG + chr5_3:26476716-26476735 Msa0806690:CDS 40.0%
TAATTCACAGTACCATGGAC+CGG - chr5_3:26476738-26476757 None:intergenic 40.0%
GATCTATTGTCCAGAAGTTC+TGG + chr5_3:26476764-26476783 Msa0806690:CDS 40.0%
GGCCATCTGCCATAAATAAT+TGG + chr5_3:26476786-26476805 Msa0806690:CDS 40.0%
GTTGTAGAATGTGCTCTTTG+TGG + chr5_3:26476824-26476843 Msa0806690:CDS 40.0%
!! GCTTTTAGGTAGTCATCTGA+TGG + chr5_3:26476860-26476879 Msa0806690:exon 40.0%
GTAGTCATCTGATGGTCAAT+GGG + chr5_3:26476868-26476887 Msa0806690:exon 40.0%
TGGGGAAAAAGAGAGAGAAG+GGG - chr5_3:26474746-26474765 None:intergenic 45.0%
CTGCTTTGCGTGTGTGTATT+TGG - chr5_3:26474848-26474867 None:intergenic 45.0%
TTGTCTTTCACCTGCTCAGA+AGG - chr5_3:26475126-26475145 None:intergenic 45.0%
! CCCTTTTCTACTCACTGAAC+TGG + chr5_3:26475385-26475404 Msa0806690:intron 45.0%
! TTTTCAGCATAGACTGCTGC+AGG + chr5_3:26475988-26476007 Msa0806690:intron 45.0%
CTTGATCTTAGAGAGGCAAC+AGG + chr5_3:26476280-26476299 Msa0806690:CDS 45.0%
AGAAAGAGTCTCTTTGGCAC+GGG - chr5_3:26476443-26476462 None:intergenic 45.0%
CAACCAGAGCATAGTAGGAT+AGG - chr5_3:26476589-26476608 None:intergenic 45.0%
! TGCTCTGGTTGCAAGGTTTT+AGG + chr5_3:26476598-26476617 Msa0806690:intron 45.0%
!!! AAGGTTTTAGGACTCATCCC+TGG + chr5_3:26476610-26476629 Msa0806690:intron 45.0%
TGAAAGAAACAACCGGTCCA+TGG + chr5_3:26476723-26476742 Msa0806690:CDS 45.0%
GTAGAATGTGCTCTTTGTGG+TGG + chr5_3:26476827-26476846 Msa0806690:CDS 45.0%
! GTGGTACTACTGGTGCTTTT+AGG + chr5_3:26476846-26476865 Msa0806690:CDS 45.0%
GGTAGTCATCTGATGGTCAA+TGG + chr5_3:26476867-26476886 Msa0806690:exon 45.0%
AAGAGTAACCTGAGCACAGA+AGG - chr5_3:26476900-26476919 None:intergenic 45.0%
! CTATCTATTTAGGCTGGCTG+TGG + chr5_3:26477255-26477274 Msa0806690:three_prime_UTR 45.0%
AAGAGAGAGAAGGGGATCGA+AGG - chr5_3:26474738-26474757 None:intergenic 50.0%
ACGTTTAACTCTGCCTGCGA+CGG + chr5_3:26474936-26474955 Msa0806690:five_prime_UTR 50.0%
CTGTTAATTGGAAGGGGCAG+TGG + chr5_3:26475755-26475774 Msa0806690:CDS 50.0%
TTGGAGGAAGCTCGTCAACT+TGG + chr5_3:26475836-26475855 Msa0806690:CDS 50.0%
AATCTCCTGGGGCAAACTCT+TGG - chr5_3:26476209-26476228 None:intergenic 50.0%
GATCTTAGAGAGGCAACAGG+AGG + chr5_3:26476283-26476302 Msa0806690:CDS 50.0%
AGTTGGATGCCTTAAGTGGG+CGG + chr5_3:26476392-26476411 Msa0806690:CDS 50.0%
GAAAGAGTCTCTTTGGCACG+GGG - chr5_3:26476442-26476461 None:intergenic 50.0%
CCTTGCAACCAGAGCATAGT+AGG - chr5_3:26476594-26476613 None:intergenic 50.0%
CCTACTATGCTCTGGTTGCA+AGG + chr5_3:26476591-26476610 Msa0806690:CDS 50.0%
GCTCTTTGTGGTGGTACTAC+TGG + chr5_3:26476836-26476855 Msa0806690:CDS 50.0%
! TCTATTTAGGCTGGCTGTGG+AGG + chr5_3:26477258-26477277 Msa0806690:three_prime_UTR 50.0%
GCGCTATCTCTTCATGCAGC+CGG + chr5_3:26474958-26474977 Msa0806690:five_prime_UTR 55.0%
TCCAAAACTCTCTCGGCACG+CGG - chr5_3:26475809-26475828 None:intergenic 55.0%
TGTTGCCAAGAGTTTGCCCC+AGG + chr5_3:26476201-26476220 Msa0806690:CDS 55.0%
GGTTCATGCCTTCTGTGCTC+AGG + chr5_3:26476889-26476908 Msa0806690:three_prime_UTR 55.0%
TGAAGAGATAGCGCCGTCGC+AGG - chr5_3:26474952-26474971 None:intergenic 60.0%
! TCCGCGTGCCGAGAGAGTTT+TGG + chr5_3:26475805-26475824 Msa0806690:CDS 60.0%
GATGCCTTAAGTGGGCGGAC+TGG + chr5_3:26476397-26476416 Msa0806690:CDS 60.0%
TGGACCAGTCCGCCCACTTA+AGG - chr5_3:26476404-26476423 None:intergenic 60.0%
!! CGGGGCATTGGCTGAGAACA+TGG - chr5_3:26476424-26476443 None:intergenic 60.0%
GTCTCTTTGGCACGGGGCAT+TGG - chr5_3:26476436-26476455 None:intergenic 60.0%
GGCAGATGGCCCAGAACTTC+TGG - chr5_3:26476777-26476796 None:intergenic 60.0%
!! CATCGCCGCGAGAGCACTTC+TGG - chr5_3:26474984-26475003 None:intergenic 65.0%
!! CAGAAGTGCTCTCGCGGCGA+TGG + chr5_3:26474982-26475001 Msa0806690:five_prime_UTR 65.0%
!! GCCGGCCAGAAGTGCTCTCG+CGG + chr5_3:26474976-26474995 Msa0806690:five_prime_UTR 70.0%
!! GCCGCGAGAGCACTTCTGGC+CGG - chr5_3:26474980-26474999 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 26474570 26477491 26474570 ID=Msa0806690;Name=Msa0806690
chr5_3 mRNA 26474570 26477491 26474570 ID=Msa0806690-mRNA-1;Parent=Msa0806690;Name=Msa0806690-mRNA-1;_AED=0.17;_eAED=0.22;_QI=569|0.4|0.5|1|0.6|0.33|6|438|250
chr5_3 exon 26474570 26475134 26474570 ID=Msa0806690-mRNA-1:exon:9615;Parent=Msa0806690-mRNA-1
chr5_3 exon 26475631 26475857 26475631 ID=Msa0806690-mRNA-1:exon:9616;Parent=Msa0806690-mRNA-1
chr5_3 exon 26476185 26476485 26476185 ID=Msa0806690-mRNA-1:exon:9617;Parent=Msa0806690-mRNA-1
chr5_3 exon 26476564 26476612 26476564 ID=Msa0806690-mRNA-1:exon:9618;Parent=Msa0806690-mRNA-1
chr5_3 exon 26476692 26476910 26476692 ID=Msa0806690-mRNA-1:exon:9619;Parent=Msa0806690-mRNA-1
chr5_3 exon 26477093 26477491 26477093 ID=Msa0806690-mRNA-1:exon:9620;Parent=Msa0806690-mRNA-1
chr5_3 five_prime_UTR 26474570 26475134 26474570 ID=Msa0806690-mRNA-1:five_prime_utr;Parent=Msa0806690-mRNA-1
chr5_3 five_prime_UTR 26475631 26475634 26475631 ID=Msa0806690-mRNA-1:five_prime_utr;Parent=Msa0806690-mRNA-1
chr5_3 CDS 26475635 26475857 26475635 ID=Msa0806690-mRNA-1:cds;Parent=Msa0806690-mRNA-1
chr5_3 CDS 26476185 26476485 26476185 ID=Msa0806690-mRNA-1:cds;Parent=Msa0806690-mRNA-1
chr5_3 CDS 26476564 26476612 26476564 ID=Msa0806690-mRNA-1:cds;Parent=Msa0806690-mRNA-1
chr5_3 CDS 26476692 26476871 26476692 ID=Msa0806690-mRNA-1:cds;Parent=Msa0806690-mRNA-1
chr5_3 three_prime_UTR 26476872 26476910 26476872 ID=Msa0806690-mRNA-1:three_prime_utr;Parent=Msa0806690-mRNA-1
chr5_3 three_prime_UTR 26477093 26477491 26477093 ID=Msa0806690-mRNA-1:three_prime_utr;Parent=Msa0806690-mRNA-1
Gene Sequence

>Msa0806690

ATGGCTTCTTTTGTTATGATATCAAATCTAAATGTATCTGTTATGATAGTTTTTGAGAGCTTTTTTGTTGTGATTTTCGGAACATGGTGTTCATGCTTAGCTTATCCAAGTCATTTTATGCTGTTAATTGGAAGGGGCAGTGGTTTTGTCGCTGTTGAGATTCTGTTTACTCCGCGTGCCGAGAGAGTTTTGGAACTCTCATTGGAGGAAGCTCGTCAACTTGAAAACTTGGTCTACAGTGTTGCCAAGAGTTTGCCCCAGGAGATTGATAAAACACATCAACAAAGATGGGATGCGGTGATTGCTAGTCAATATCTGCTTGATCTTAGAGAGGCAACAGGAGGTCGTATCCACTTACTTCTTTTGCAATTTCTTTTAACAGTTTGCATACCGTATAACAGTAAAAATATGATTTTTCAAAATTCATTGAAGTTGGATGCCTTAAGTGGGCGGACTGGTCCATGTTCTCAGCCAATGCCCCGTGCCAAAGAGACTCTTTCTAGAGTTGCTGTCACAAGAGCTTCACGATTTGAGAATGTGTTAAATCCTATCCTACTATGCTCTGGTTGCAAGGTTGCGGTGCATTCAGTTTGCTATCGTAGTGTGAAAGAAACAACCGGTCCATGGTACTGTGAATTATGTGAAGATCTATTGTCCAGAAGTTCTGGGCCATCTGCCATAAATAATTGGGAGAAGCCATATTTTGTTGTAGAATGTGCTCTTTGTGGTGGTACTACTGGTGCTTTTAGGTAG
Protein sequence

>Msa0806690

MASFVMISNLNVSVMIVFESFFVVIFGTWCSCLAYPSHFMLLIGRGSGFVAVEILFTPRAERVLELSLEEARQLENLVYSVAKSLPQEIDKTHQQRWDAVIASQYLLDLREATGGRIHLLLLQFLLTVCIPYNSKNMIFQNSLKLDALSGRTGPCSQPMPRAKETLSRVAVTRASRFENVLNPILLCSGCKVAVHSVCYRSVKETTGPWYCELCEDLLSRSSGPSAINNWEKPYFVVECALCGGTTGAFR*