Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1374100 | Msa1382600 | 0.896354 | 3.999884e-76 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1382600 | MtrunA17_Chr1g0155331 | 96.296 | 27 | 1 | 0 | 27 | 53 | 135 | 161 | 2.29e-12 | 58.5 |
| Msa1382600 | MtrunA17_Chr2g0298841 | 96.296 | 27 | 1 | 0 | 27 | 53 | 135 | 161 | 2.46e-12 | 58.5 |
| Msa1382600 | MtrunA17_Chr4g0002321 | 92.593 | 27 | 2 | 0 | 27 | 53 | 134 | 160 | 6.81e-12 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 128 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCTGACGCTCTTCAGATTA+GGG | 0.318959 | tig0020520:+62259 | None:intergenic |
| AATAATTACTGAAATCCTTA+TGG | 0.356825 | tig0020520:+63304 | None:intergenic |
| TCTTCAAATAAAGGTTCAAC+TGG | 0.368005 | tig0020520:+63281 | None:intergenic |
| TCCCTGACGCTCTTCAGATT+AGG | 0.391787 | tig0020520:+62258 | None:intergenic |
| ACCCTAATCTGAAGAGCGTC+AGG | 0.410603 | tig0020520:-62260 | Msa1382600:CDS |
| ACAAGCAGATTTCATCGAAC+AGG | 0.426738 | tig0020520:-62203 | None:intergenic |
| TTGATCTACAAGAGGGGATA+CGG | 0.470817 | tig0020520:-62234 | Msa1382600:CDS |
| GGAATTGCTTATGTTACATA+CGG | 0.485132 | tig0020520:-62285 | Msa1382600:CDS |
| GTTCGATGATGAATGCCATA+AGG | 0.503644 | tig0020520:-63319 | None:intergenic |
| TATTTGAAGAATGTGAAAAG+AGG | 0.510086 | tig0020520:-63268 | Msa1382600:intron |
| GAATTGCTTATGTTACATAC+GGG | 0.584032 | tig0020520:-62284 | Msa1382600:CDS |
| CAGGGAATTGATCTACAAGA+GGG | 0.612367 | tig0020520:-62241 | Msa1382600:CDS |
| TCAGGGAATTGATCTACAAG+AGG | 0.639447 | tig0020520:-62242 | Msa1382600:CDS |
| CCCTAATCTGAAGAGCGTCA+GGG | 0.672345 | tig0020520:-62259 | Msa1382600:CDS |
| AGGGAATTGATCTACAAGAG+GGG | 0.716288 | tig0020520:-62240 | Msa1382600:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGTAAATTAGAAACTAATT+TGG | + | tig0020520:62293-62312 | None:intergenic | 15.0% |
| !!! | TTTTTTACAATAAGTTAGAT+TGG | + | tig0020520:62853-62872 | None:intergenic | 15.0% |
| !! | AATCTAACTTATTGTAAAAA+AGG | - | tig0020520:62852-62871 | Msa1382600:intron | 15.0% |
| !!! | TGAATATTTTAACCATTAAT+AGG | - | tig0020520:63125-63144 | Msa1382600:intron | 15.0% |
| !! | ATGTAAATTAGAAACTAATT+TGG | + | tig0020520:62293-62312 | None:intergenic | 15.0% |
| !!! | TTTTTTACAATAAGTTAGAT+TGG | + | tig0020520:62853-62872 | None:intergenic | 15.0% |
| !! | AATCTAACTTATTGTAAAAA+AGG | - | tig0020520:62852-62871 | Msa1382600:intron | 15.0% |
| !!! | TGAATATTTTAACCATTAAT+AGG | - | tig0020520:63125-63144 | Msa1382600:intron | 15.0% |
| !! | AATAATTACTGAAATCCTTA+TGG | + | tig0020520:62216-62235 | None:intergenic | 20.0% |
| !!! | TTTTCACATTCTTCAAATAA+AGG | + | tig0020520:62248-62267 | None:intergenic | 20.0% |
| !! | CAATCATAAAAACTTTCATA+GGG | + | tig0020520:62559-62578 | None:intergenic | 20.0% |
| !! | ATTCATAAAAACTTTGCATA+GGG | + | tig0020520:62591-62610 | None:intergenic | 20.0% |
| !! | AAACAAAAACTATTGAACAT+TGG | - | tig0020520:62739-62758 | Msa1382600:intron | 20.0% |
| !! | ATAAGTTTACAAAACAACAA+AGG | + | tig0020520:62878-62897 | None:intergenic | 20.0% |
| !! | AATAATTACTGAAATCCTTA+TGG | + | tig0020520:62216-62235 | None:intergenic | 20.0% |
| !!! | TTTTCACATTCTTCAAATAA+AGG | + | tig0020520:62248-62267 | None:intergenic | 20.0% |
| !! | CAATCATAAAAACTTTCATA+GGG | + | tig0020520:62559-62578 | None:intergenic | 20.0% |
| !! | ATTCATAAAAACTTTGCATA+GGG | + | tig0020520:62591-62610 | None:intergenic | 20.0% |
| !! | AAACAAAAACTATTGAACAT+TGG | - | tig0020520:62739-62758 | Msa1382600:intron | 20.0% |
| !! | ATAAGTTTACAAAACAACAA+AGG | + | tig0020520:62878-62897 | None:intergenic | 20.0% |
| ! | TATTTGAAGAATGTGAAAAG+AGG | - | tig0020520:62249-62268 | Msa1382600:CDS | 25.0% |
| ! | TGTGAATACATCTATTCTTT+AGG | - | tig0020520:62379-62398 | Msa1382600:intron | 25.0% |
| !!! | TACATCTATTCTTTAGGATT+AGG | - | tig0020520:62385-62404 | Msa1382600:intron | 25.0% |
| ! | AACAGCATTATGTAATACAT+AGG | + | tig0020520:62417-62436 | None:intergenic | 25.0% |
| ! | GCAATCATAAAAACTTTCAT+AGG | + | tig0020520:62560-62579 | None:intergenic | 25.0% |
| ! | CATTCATAAAAACTTTGCAT+AGG | + | tig0020520:62592-62611 | None:intergenic | 25.0% |
| !! | CTATGCAAAGTTTTTATGAA+TGG | - | tig0020520:62590-62609 | Msa1382600:intron | 25.0% |
| ! | CATTAATGCACTATTGTTAA+TGG | + | tig0020520:62811-62830 | None:intergenic | 25.0% |
| !! | TGTTATTTGTGACAATGATT+AGG | - | tig0020520:62973-62992 | Msa1382600:intron | 25.0% |
| ! | TATTTGAAGAATGTGAAAAG+AGG | - | tig0020520:62249-62268 | Msa1382600:CDS | 25.0% |
| ! | TGTGAATACATCTATTCTTT+AGG | - | tig0020520:62379-62398 | Msa1382600:intron | 25.0% |
| !!! | TACATCTATTCTTTAGGATT+AGG | - | tig0020520:62385-62404 | Msa1382600:intron | 25.0% |
| ! | AACAGCATTATGTAATACAT+AGG | + | tig0020520:62417-62436 | None:intergenic | 25.0% |
| ! | GCAATCATAAAAACTTTCAT+AGG | + | tig0020520:62560-62579 | None:intergenic | 25.0% |
| ! | CATTCATAAAAACTTTGCAT+AGG | + | tig0020520:62592-62611 | None:intergenic | 25.0% |
| !! | CTATGCAAAGTTTTTATGAA+TGG | - | tig0020520:62590-62609 | Msa1382600:intron | 25.0% |
| ! | CATTAATGCACTATTGTTAA+TGG | + | tig0020520:62811-62830 | None:intergenic | 25.0% |
| !! | TGTTATTTGTGACAATGATT+AGG | - | tig0020520:62973-62992 | Msa1382600:intron | 25.0% |
| TCTTCAAATAAAGGTTCAAC+TGG | + | tig0020520:62239-62258 | None:intergenic | 30.0% | |
| ! | GCTTGAACAAAGAATTTTGT+TGG | + | tig0020520:62348-62367 | None:intergenic | 30.0% |
| TGAACTCATAATCAGAATGT+GGG | + | tig0020520:62503-62522 | None:intergenic | 30.0% | |
| TTGAACTCATAATCAGAATG+TGG | + | tig0020520:62504-62523 | None:intergenic | 30.0% | |
| ACAAATGACACCTGAATATT+TGG | + | tig0020520:62686-62705 | None:intergenic | 30.0% | |
| !! | AAATATTCAGGTGTCATTTG+TGG | - | tig0020520:62685-62704 | Msa1382600:intron | 30.0% |
| CAATAGTGCATTAATGTCAT+TGG | - | tig0020520:62815-62834 | Msa1382600:intron | 30.0% | |
| TATTTGTGACAATGATTAGG+AGG | - | tig0020520:62976-62995 | Msa1382600:intron | 30.0% | |
| ATTTGTGACAATGATTAGGA+GGG | - | tig0020520:62977-62996 | Msa1382600:intron | 30.0% | |
| !! | ATAAATCTTTGCTGGTTTGA+AGG | - | tig0020520:63052-63071 | Msa1382600:intron | 30.0% |
| ACACAAAGAAAGCCTATTAA+TGG | + | tig0020520:63140-63159 | None:intergenic | 30.0% | |
| ! | CTTTATTTCACTTCCTTCTT+TGG | + | tig0020520:63208-63227 | None:intergenic | 30.0% |
| CAAAGAAGGAAGTGAAATAA+AGG | - | tig0020520:63206-63225 | Msa1382600:intron | 30.0% | |
| AAGGAAGTGAAATAAAGGAA+AGG | - | tig0020520:63211-63230 | Msa1382600:intron | 30.0% | |
| GGAATTGCTTATGTTACATA+CGG | - | tig0020520:63232-63251 | Msa1382600:intron | 30.0% | |
| GAATTGCTTATGTTACATAC+GGG | - | tig0020520:63233-63252 | Msa1382600:intron | 30.0% | |
| TCTTCAAATAAAGGTTCAAC+TGG | + | tig0020520:62239-62258 | None:intergenic | 30.0% | |
| ! | GCTTGAACAAAGAATTTTGT+TGG | + | tig0020520:62348-62367 | None:intergenic | 30.0% |
| TGAACTCATAATCAGAATGT+GGG | + | tig0020520:62503-62522 | None:intergenic | 30.0% | |
| TTGAACTCATAATCAGAATG+TGG | + | tig0020520:62504-62523 | None:intergenic | 30.0% | |
| ACAAATGACACCTGAATATT+TGG | + | tig0020520:62686-62705 | None:intergenic | 30.0% | |
| !! | AAATATTCAGGTGTCATTTG+TGG | - | tig0020520:62685-62704 | Msa1382600:intron | 30.0% |
| CAATAGTGCATTAATGTCAT+TGG | - | tig0020520:62815-62834 | Msa1382600:intron | 30.0% | |
| TATTTGTGACAATGATTAGG+AGG | - | tig0020520:62976-62995 | Msa1382600:intron | 30.0% | |
| ATTTGTGACAATGATTAGGA+GGG | - | tig0020520:62977-62996 | Msa1382600:intron | 30.0% | |
| !! | ATAAATCTTTGCTGGTTTGA+AGG | - | tig0020520:63052-63071 | Msa1382600:intron | 30.0% |
| ACACAAAGAAAGCCTATTAA+TGG | + | tig0020520:63140-63159 | None:intergenic | 30.0% | |
| ! | CTTTATTTCACTTCCTTCTT+TGG | + | tig0020520:63208-63227 | None:intergenic | 30.0% |
| CAAAGAAGGAAGTGAAATAA+AGG | - | tig0020520:63206-63225 | Msa1382600:intron | 30.0% | |
| AAGGAAGTGAAATAAAGGAA+AGG | - | tig0020520:63211-63230 | Msa1382600:intron | 30.0% | |
| GGAATTGCTTATGTTACATA+CGG | - | tig0020520:63232-63251 | Msa1382600:intron | 30.0% | |
| GAATTGCTTATGTTACATAC+GGG | - | tig0020520:63233-63252 | Msa1382600:intron | 30.0% | |
| CAGATATGTTGTGGAATGAA+TGG | - | tig0020520:62452-62471 | Msa1382600:intron | 35.0% | |
| AAACTTCAATCTGCCATGTT+TGG | - | tig0020520:62635-62654 | Msa1382600:intron | 35.0% | |
| CATTGAACATGCTTGTAACT+TGG | - | tig0020520:62931-62950 | Msa1382600:intron | 35.0% | |
| GTTAGCTGATAAATCTTTGC+TGG | - | tig0020520:63044-63063 | Msa1382600:intron | 35.0% | |
| GATTCATTGTTCATGGAAAG+AGG | + | tig0020520:63184-63203 | None:intergenic | 35.0% | |
| ! | CTTTGGAGATTCATTGTTCA+TGG | + | tig0020520:63191-63210 | None:intergenic | 35.0% |
| GAACAATGAATCTCCAAAGA+AGG | - | tig0020520:63192-63211 | Msa1382600:intron | 35.0% | |
| CAGATATGTTGTGGAATGAA+TGG | - | tig0020520:62452-62471 | Msa1382600:intron | 35.0% | |
| AAACTTCAATCTGCCATGTT+TGG | - | tig0020520:62635-62654 | Msa1382600:intron | 35.0% | |
| CATTGAACATGCTTGTAACT+TGG | - | tig0020520:62931-62950 | Msa1382600:intron | 35.0% | |
| GTTAGCTGATAAATCTTTGC+TGG | - | tig0020520:63044-63063 | Msa1382600:intron | 35.0% | |
| GATTCATTGTTCATGGAAAG+AGG | + | tig0020520:63184-63203 | None:intergenic | 35.0% | |
| ! | CTTTGGAGATTCATTGTTCA+TGG | + | tig0020520:63191-63210 | None:intergenic | 35.0% |
| GAACAATGAATCTCCAAAGA+AGG | - | tig0020520:63192-63211 | Msa1382600:intron | 35.0% | |
| TGCTTGTGTCAGATATGTTG+TGG | - | tig0020520:62443-62462 | Msa1382600:intron | 40.0% | |
| ! | TTCATAGGGCTTTTGCTTTC+TGG | + | tig0020520:62545-62564 | None:intergenic | 40.0% |
| CTCCATGTCTCCAAATATTC+AGG | - | tig0020520:62673-62692 | Msa1382600:intron | 40.0% | |
| CACCTGAATATTTGGAGACA+TGG | + | tig0020520:62678-62697 | None:intergenic | 40.0% | |
| ! | ACTGCAAACTTGTTCTCCAA+GGG | + | tig0020520:63010-63029 | None:intergenic | 40.0% |
| TCAGGGAATTGATCTACAAG+AGG | - | tig0020520:63275-63294 | Msa1382600:CDS | 40.0% | |
| CAGGGAATTGATCTACAAGA+GGG | - | tig0020520:63276-63295 | Msa1382600:CDS | 40.0% | |
| AGGGAATTGATCTACAAGAG+GGG | - | tig0020520:63277-63296 | Msa1382600:CDS | 40.0% | |
| TTGATCTACAAGAGGGGATA+CGG | - | tig0020520:63283-63302 | Msa1382600:CDS | 40.0% | |
| TGCTTGTGTCAGATATGTTG+TGG | - | tig0020520:62443-62462 | Msa1382600:intron | 40.0% | |
| ! | TTCATAGGGCTTTTGCTTTC+TGG | + | tig0020520:62545-62564 | None:intergenic | 40.0% |
| CTCCATGTCTCCAAATATTC+AGG | - | tig0020520:62673-62692 | Msa1382600:intron | 40.0% | |
| CACCTGAATATTTGGAGACA+TGG | + | tig0020520:62678-62697 | None:intergenic | 40.0% | |
| ! | ACTGCAAACTTGTTCTCCAA+GGG | + | tig0020520:63010-63029 | None:intergenic | 40.0% |
| TCAGGGAATTGATCTACAAG+AGG | - | tig0020520:63275-63294 | Msa1382600:CDS | 40.0% | |
| CAGGGAATTGATCTACAAGA+GGG | - | tig0020520:63276-63295 | Msa1382600:CDS | 40.0% | |
| AGGGAATTGATCTACAAGAG+GGG | - | tig0020520:63277-63296 | Msa1382600:CDS | 40.0% | |
| TTGATCTACAAGAGGGGATA+CGG | - | tig0020520:63283-63302 | Msa1382600:CDS | 40.0% | |
| ! | CAATCTGCCATGTTTGGTGA+AGG | - | tig0020520:62641-62660 | Msa1382600:intron | 45.0% |
| AAGAGGACCTTCACCAAACA+TGG | + | tig0020520:62651-62670 | None:intergenic | 45.0% | |
| TTTGGAGACATGGAGTCAAG+AGG | + | tig0020520:62668-62687 | None:intergenic | 45.0% | |
| TTAGGAGGGAACTTATCCCT+TGG | - | tig0020520:62991-63010 | Msa1382600:intron | 45.0% | |
| ! | GACTGCAAACTTGTTCTCCA+AGG | + | tig0020520:63011-63030 | None:intergenic | 45.0% |
| TGGAGAACAAGTTTGCAGTC+AGG | - | tig0020520:63011-63030 | Msa1382600:intron | 45.0% | |
| ! | CAATCTGCCATGTTTGGTGA+AGG | - | tig0020520:62641-62660 | Msa1382600:intron | 45.0% |
| AAGAGGACCTTCACCAAACA+TGG | + | tig0020520:62651-62670 | None:intergenic | 45.0% | |
| TTTGGAGACATGGAGTCAAG+AGG | + | tig0020520:62668-62687 | None:intergenic | 45.0% | |
| TTAGGAGGGAACTTATCCCT+TGG | - | tig0020520:62991-63010 | Msa1382600:intron | 45.0% | |
| ! | GACTGCAAACTTGTTCTCCA+AGG | + | tig0020520:63011-63030 | None:intergenic | 45.0% |
| TGGAGAACAAGTTTGCAGTC+AGG | - | tig0020520:63011-63030 | Msa1382600:intron | 45.0% | |
| !! | AAGAAATATTTAAATTTAAA+CGG | - | tig0020520:62774-62793 | Msa1382600:intron | 5.0% |
| !! | AAGAAATATTTAAATTTAAA+CGG | - | tig0020520:62774-62793 | Msa1382600:intron | 5.0% |
| ! | TGTTTCTGACCTCTGCGAGA+AGG | - | tig0020520:62476-62495 | Msa1382600:intron | 50.0% |
| !! | ACCCTAATCTGAAGAGCGTC+AGG | - | tig0020520:63257-63276 | Msa1382600:intron | 50.0% |
| CCCTGACGCTCTTCAGATTA+GGG | + | tig0020520:63261-63280 | None:intergenic | 50.0% | |
| !! | CCCTAATCTGAAGAGCGTCA+GGG | - | tig0020520:63258-63277 | Msa1382600:intron | 50.0% |
| TCCCTGACGCTCTTCAGATT+AGG | + | tig0020520:63262-63281 | None:intergenic | 50.0% | |
| ! | TGTTTCTGACCTCTGCGAGA+AGG | - | tig0020520:62476-62495 | Msa1382600:intron | 50.0% |
| !! | ACCCTAATCTGAAGAGCGTC+AGG | - | tig0020520:63257-63276 | Msa1382600:intron | 50.0% |
| CCCTGACGCTCTTCAGATTA+GGG | + | tig0020520:63261-63280 | None:intergenic | 50.0% | |
| !! | CCCTAATCTGAAGAGCGTCA+GGG | - | tig0020520:63258-63277 | Msa1382600:intron | 50.0% |
| TCCCTGACGCTCTTCAGATT+AGG | + | tig0020520:63262-63281 | None:intergenic | 50.0% | |
| AATGTGGGACCTTCTCGCAG+AGG | + | tig0020520:62488-62507 | None:intergenic | 55.0% | |
| AATGTGGGACCTTCTCGCAG+AGG | + | tig0020520:62488-62507 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0020520 | gene | 62206 | 63333 | 62206 | ID=Msa1382600;Name=Msa1382600 |
| tig0020520 | mRNA | 62206 | 63333 | 62206 | ID=Msa1382600-mRNA-1;Parent=Msa1382600;Name=Msa1382600-mRNA-1;_AED=0.63;_eAED=0.64;_QI=0|0|0|1|0|0|2|0|57 |
| tig0020520 | exon | 63269 | 63333 | 63269 | ID=Msa1382600-mRNA-1:exon:8237;Parent=Msa1382600-mRNA-1 |
| tig0020520 | exon | 62206 | 62311 | 62206 | ID=Msa1382600-mRNA-1:exon:8236;Parent=Msa1382600-mRNA-1 |
| tig0020520 | CDS | 63269 | 63333 | 63269 | ID=Msa1382600-mRNA-1:cds;Parent=Msa1382600-mRNA-1 |
| tig0020520 | CDS | 62206 | 62311 | 62206 | ID=Msa1382600-mRNA-1:cds;Parent=Msa1382600-mRNA-1 |
| Gene Sequence |
| Protein sequence |
