Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1385140 | XP_039689998.1 | 93.525 | 139 | 9 | 0 | 1 | 139 | 1 | 139 | 5.90e-82 | 247 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1385140 | A0A396HQF8 | 93.525 | 139 | 9 | 0 | 1 | 139 | 1 | 139 | 4.41e-82 | 248 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0798700 | Msa1385140 | 0.963254 | 7.342092e-122 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1385140 | MtrunA17_Chr5g0403181 | 93.525 | 139 | 9 | 0 | 1 | 139 | 1 | 139 | 8.49e-86 | 248 |
| Msa1385140 | MtrunA17_Chr4g0034291 | 42.529 | 87 | 47 | 1 | 53 | 139 | 25 | 108 | 1.45e-15 | 68.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAGAGATGCTAGTGAAAA+TGG | 0.262061 | tig0020715:+35470 | Msa1385140:CDS |
| TCTTGCAAGCATTGTTGTTT+CGG | 0.326610 | tig0020715:+35601 | Msa1385140:CDS |
| CATTTCTAATGGTGCAATCA+AGG | 0.436633 | tig0020715:+35562 | Msa1385140:CDS |
| ACAACAATGCTTGCAAGAAC+CGG | 0.444006 | tig0020715:-35596 | None:intergenic |
| TTAACGCTATTAACTTACAT+TGG | 0.460517 | tig0020715:-35819 | None:intergenic |
| TCGAAGACCATCATCGATCA+TGG | 0.465762 | tig0020715:+35532 | Msa1385140:CDS |
| ATGGTCATGAACATTTCTAA+TGG | 0.483592 | tig0020715:+35551 | Msa1385140:CDS |
| ATGCAGAATCAATGTGCGTA+GGG | 0.484037 | tig0020715:+35657 | Msa1385140:CDS |
| GGATAATTAGAATTCGAGAT+AGG | 0.487165 | tig0020715:-35743 | None:intergenic |
| AGATGCTAGTGAAAATGGTT+TGG | 0.505903 | tig0020715:+35475 | Msa1385140:CDS |
| AATCAAGGAATACAAGAAAC+CGG | 0.545666 | tig0020715:+35577 | Msa1385140:CDS |
| CATAGAAGTTGAAGAGACAA+TGG | 0.576223 | tig0020715:-35764 | None:intergenic |
| TTCATGACCATGATCGATGA+TGG | 0.589478 | tig0020715:-35539 | None:intergenic |
| AATGCAGAATCAATGTGCGT+AGG | 0.594399 | tig0020715:+35656 | Msa1385140:CDS |
| TTAACTTACATTGGAAAGTG+CGG | 0.612363 | tig0020715:-35810 | None:intergenic |
| TCGTCTTCATCAGAAACTCG+TGG | 0.662517 | tig0020715:-35689 | None:intergenic |
| TGCAGAATCAATGTGCGTAG+GGG | 0.674490 | tig0020715:+35658 | Msa1385140:CDS |
| ACAATGAAGTAGATTCGACC+CGG | 0.685668 | tig0020715:-35719 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGGTCATGAACATTTCTAA+TGG | + | tig0020715:35551-35570 | Msa1385140:CDS | 30.0% | |
| AATCAAGGAATACAAGAAAC+CGG | + | tig0020715:35577-35596 | Msa1385140:CDS | 30.0% | |
| GGATAATTAGAATTCGAGAT+AGG | - | tig0020715:35746-35765 | None:intergenic | 30.0% | |
| TTAACTTACATTGGAAAGTG+CGG | - | tig0020715:35813-35832 | None:intergenic | 30.0% | |
| ACAAGAGATGCTAGTGAAAA+TGG | + | tig0020715:35470-35489 | Msa1385140:CDS | 35.0% | |
| AGATGCTAGTGAAAATGGTT+TGG | + | tig0020715:35475-35494 | Msa1385140:CDS | 35.0% | |
| CATTTCTAATGGTGCAATCA+AGG | + | tig0020715:35562-35581 | Msa1385140:CDS | 35.0% | |
| !! | TCTTGCAAGCATTGTTGTTT+CGG | + | tig0020715:35601-35620 | Msa1385140:CDS | 35.0% |
| CATAGAAGTTGAAGAGACAA+TGG | - | tig0020715:35767-35786 | None:intergenic | 35.0% | |
| TTCATGACCATGATCGATGA+TGG | - | tig0020715:35542-35561 | None:intergenic | 40.0% | |
| ACAACAATGCTTGCAAGAAC+CGG | - | tig0020715:35599-35618 | None:intergenic | 40.0% | |
| AATGCAGAATCAATGTGCGT+AGG | + | tig0020715:35656-35675 | Msa1385140:CDS | 40.0% | |
| ATGCAGAATCAATGTGCGTA+GGG | + | tig0020715:35657-35676 | Msa1385140:CDS | 40.0% | |
| ! | TGATGAAGACGAGCTTTTAC+CGG | + | tig0020715:35700-35719 | Msa1385140:CDS | 40.0% |
| ACAATGAAGTAGATTCGACC+CGG | - | tig0020715:35722-35741 | None:intergenic | 40.0% | |
| TCGAAGACCATCATCGATCA+TGG | + | tig0020715:35532-35551 | Msa1385140:CDS | 45.0% | |
| TGCAGAATCAATGTGCGTAG+GGG | + | tig0020715:35658-35677 | Msa1385140:CDS | 45.0% | |
| TCGTCTTCATCAGAAACTCG+TGG | - | tig0020715:35692-35711 | None:intergenic | 45.0% | |
| ! | GATGAAGACGAGCTTTTACC+GGG | + | tig0020715:35701-35720 | Msa1385140:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0020715 | gene | 35407 | 35832 | 35407 | ID=Msa1385140;Name=Msa1385140 |
| tig0020715 | mRNA | 35407 | 35832 | 35407 | ID=Msa1385140-mRNA-1;Parent=Msa1385140;Name=Msa1385140-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|-1|0|1|-1|1|1|0|141 |
| tig0020715 | exon | 35407 | 35832 | 35407 | ID=Msa1385140-mRNA-1:exon:9462;Parent=Msa1385140-mRNA-1 |
| tig0020715 | CDS | 35407 | 35832 | 35407 | ID=Msa1385140-mRNA-1:cds;Parent=Msa1385140-mRNA-1 |
| Gene Sequence |
| Protein sequence |
