Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1386550 | XP_003611549.2 | 87.500 | 104 | 11 | 1 | 1 | 104 | 1 | 102 | 3.30e-55 | 177 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1386550 | G7K2F6 | 87.500 | 104 | 11 | 1 | 1 | 104 | 1 | 102 | 1.57e-55 | 177 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0797650 | Msa1386550 | 0.830019 | 3.716529e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1386550 | MtrunA17_Chr5g0400961 | 87.500 | 104 | 11 | 1 | 1 | 104 | 1 | 102 | 3.03e-59 | 177 |
| Msa1386550 | MtrunA17_Chr4g0054241 | 33.553 | 152 | 54 | 5 | 9 | 115 | 32 | 181 | 3.37e-14 | 65.5 |
| Msa1386550 | MtrunA17_Chr4g0054231 | 53.226 | 62 | 28 | 1 | 9 | 69 | 31 | 92 | 2.52e-12 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1386550 | AT2G22880.1 | 38.462 | 117 | 58 | 5 | 1 | 117 | 1 | 103 | 5.23e-12 | 58.5 |
Find 24 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAACCCTAACTGCTGTTAA+AGG | 0.195754 | tig0020830:+84674 | None:intergenic |
| GACAAAGCAATCAGGTTTAT+TGG | 0.268685 | tig0020830:-84504 | Msa1386550:CDS |
| TGTTGTTGAGGCTTGAATTC+TGG | 0.279950 | tig0020830:+84599 | None:intergenic |
| GCACCTGTTGTTAGTGATAA+AGG | 0.285706 | tig0020830:-84554 | Msa1386550:CDS |
| CACCTGTTGTTAGTGATAAA+GGG | 0.389424 | tig0020830:-84553 | Msa1386550:CDS |
| CACAACCTTTAACAGCAGTT+AGG | 0.395073 | tig0020830:-84679 | Msa1386550:CDS |
| CTTCACCATCTTCTCAGAGT+TGG | 0.407398 | tig0020830:-84469 | Msa1386550:CDS |
| CACCATCTTCTCAGAGTTGG+TGG | 0.431356 | tig0020830:-84466 | Msa1386550:CDS |
| ATTATGAGACTTAGGATAAG+AGG | 0.439722 | tig0020830:+84766 | None:intergenic |
| ACAACCTTTAACAGCAGTTA+GGG | 0.458936 | tig0020830:-84678 | Msa1386550:CDS |
| AATGAATTATTATGAGACTT+AGG | 0.459794 | tig0020830:+84758 | None:intergenic |
| ACCCCTTTATCACTAACAAC+AGG | 0.485193 | tig0020830:+84551 | None:intergenic |
| AGTGGTGTGACAAAGCAATC+AGG | 0.507552 | tig0020830:-84512 | Msa1386550:CDS |
| CTAACTGCTGTTAAAGGTTG+TGG | 0.514313 | tig0020830:+84680 | None:intergenic |
| GACCACCAACTCTGAGAAGA+TGG | 0.520594 | tig0020830:+84464 | None:intergenic |
| ATTGTGGCTTTCTAACGGAA+TGG | 0.533782 | tig0020830:+84732 | None:intergenic |
| AGACTTAGGATAAGAGGTGG+TGG | 0.557065 | tig0020830:+84772 | None:intergenic |
| GGTGGTGGAATAAGCTTCCA+TGG | 0.561828 | tig0020830:+84787 | None:intergenic |
| ACAAATTGTTGATGTTGTTG+AGG | 0.565750 | tig0020830:+84587 | None:intergenic |
| GAAAGCCACAATCAAAACCA+TGG | 0.621885 | tig0020830:-84721 | Msa1386550:CDS |
| GAGTTAGTTCAACAACTCAC+AGG | 0.631511 | tig0020830:-84626 | Msa1386550:CDS |
| ACCTGTTGTTAGTGATAAAG+GGG | 0.645367 | tig0020830:-84552 | Msa1386550:CDS |
| GTGCAATATCAAGAGAGTAG+TGG | 0.659499 | tig0020830:-84530 | Msa1386550:CDS |
| ATGAGACTTAGGATAAGAGG+TGG | 0.667730 | tig0020830:+84769 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAATTATTATGAGACTT+AGG | + | tig0020830:84481-84500 | None:intergenic | 20.0% |
| ATTATGAGACTTAGGATAAG+AGG | + | tig0020830:84473-84492 | None:intergenic | 30.0% | |
| !! | TTTTGATTGTGGCTTTCTAA+CGG | + | tig0020830:84512-84531 | None:intergenic | 30.0% |
| !!! | TTTTTCCATGGTTTTGATTG+TGG | + | tig0020830:84523-84542 | None:intergenic | 30.0% |
| ACAAATTGTTGATGTTGTTG+AGG | + | tig0020830:84652-84671 | None:intergenic | 30.0% | |
| ACAACCTTTAACAGCAGTTA+GGG | - | tig0020830:84558-84577 | Msa1386550:CDS | 35.0% | |
| TAAACCCTAACTGCTGTTAA+AGG | + | tig0020830:84565-84584 | None:intergenic | 35.0% | |
| CACCTGTTGTTAGTGATAAA+GGG | - | tig0020830:84683-84702 | Msa1386550:CDS | 35.0% | |
| ACCTGTTGTTAGTGATAAAG+GGG | - | tig0020830:84684-84703 | Msa1386550:CDS | 35.0% | |
| GACAAAGCAATCAGGTTTAT+TGG | - | tig0020830:84732-84751 | Msa1386550:CDS | 35.0% | |
| ATGAGACTTAGGATAAGAGG+TGG | + | tig0020830:84470-84489 | None:intergenic | 40.0% | |
| ATTGTGGCTTTCTAACGGAA+TGG | + | tig0020830:84507-84526 | None:intergenic | 40.0% | |
| GAAAGCCACAATCAAAACCA+TGG | - | tig0020830:84515-84534 | Msa1386550:CDS | 40.0% | |
| ACCATGGAAAAAAGCTTCAG+TGG | - | tig0020830:84531-84550 | Msa1386550:CDS | 40.0% | |
| CTAACTGCTGTTAAAGGTTG+TGG | + | tig0020830:84559-84578 | None:intergenic | 40.0% | |
| CACAACCTTTAACAGCAGTT+AGG | - | tig0020830:84557-84576 | Msa1386550:CDS | 40.0% | |
| GAGTTAGTTCAACAACTCAC+AGG | - | tig0020830:84610-84629 | Msa1386550:CDS | 40.0% | |
| TGTTGTTGAGGCTTGAATTC+TGG | + | tig0020830:84640-84659 | None:intergenic | 40.0% | |
| GCACCTGTTGTTAGTGATAA+AGG | - | tig0020830:84682-84701 | Msa1386550:CDS | 40.0% | |
| ACCCCTTTATCACTAACAAC+AGG | + | tig0020830:84688-84707 | None:intergenic | 40.0% | |
| GTGCAATATCAAGAGAGTAG+TGG | - | tig0020830:84706-84725 | Msa1386550:CDS | 40.0% | |
| AGACTTAGGATAAGAGGTGG+TGG | + | tig0020830:84467-84486 | None:intergenic | 45.0% | |
| !!! | GCCACTGAAGCTTTTTTCCA+TGG | + | tig0020830:84535-84554 | None:intergenic | 45.0% |
| AGTGGTGTGACAAAGCAATC+AGG | - | tig0020830:84724-84743 | Msa1386550:CDS | 45.0% | |
| CTTCACCATCTTCTCAGAGT+TGG | - | tig0020830:84767-84786 | Msa1386550:CDS | 45.0% | |
| CACCATCTTCTCAGAGTTGG+TGG | - | tig0020830:84770-84789 | Msa1386550:CDS | 50.0% | |
| ! | GACCACCAACTCTGAGAAGA+TGG | + | tig0020830:84775-84794 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0020830 | gene | 84451 | 84807 | 84451 | ID=Msa1386550;Name=Msa1386550 |
| tig0020830 | mRNA | 84451 | 84807 | 84451 | ID=Msa1386550-mRNA-1;Parent=Msa1386550;Name=Msa1386550-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|118 |
| tig0020830 | exon | 84451 | 84807 | 84451 | ID=Msa1386550-mRNA-1:exon:10242;Parent=Msa1386550-mRNA-1 |
| tig0020830 | CDS | 84451 | 84807 | 84451 | ID=Msa1386550-mRNA-1:cds;Parent=Msa1386550-mRNA-1 |
| Gene Sequence |
| Protein sequence |
