Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0797650 | XP_003611549.2 | 86.538 | 104 | 12 | 1 | 1 | 104 | 1 | 102 | 2.62e-54 | 176 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0797650 | G7K2F6 | 86.538 | 104 | 12 | 1 | 1 | 104 | 1 | 102 | 1.25e-54 | 176 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0698280 | Msa0797650 | 0.805482 | 1.306947e-49 | -8.615850e-47 |
| Msa0797650 | Msa1162890 | 0.802561 | 5.292546e-49 | -8.615850e-47 |
| Msa0797650 | Msa1386550 | 0.830019 | 3.716529e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0797650 | MtrunA17_Chr5g0400961 | 86.538 | 104 | 12 | 1 | 1 | 104 | 1 | 102 | 2.41e-58 | 176 |
| Msa0797650 | MtrunA17_Chr4g0054241 | 54.839 | 62 | 27 | 1 | 9 | 69 | 32 | 93 | 2.62e-14 | 65.9 |
| Msa0797650 | MtrunA17_Chr4g0054231 | 54.839 | 62 | 27 | 1 | 9 | 69 | 31 | 92 | 2.94e-13 | 62.8 |
| Msa0797650 | MtrunA17_Chr4g0054251 | 56.364 | 55 | 23 | 1 | 16 | 69 | 35 | 89 | 2.55e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0797650 | AT2G22880.1 | 37.607 | 117 | 59 | 4 | 1 | 117 | 1 | 103 | 1.89e-11 | 57.0 |
Find 22 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAACCCTAACTGCTGTTAA+AGG | 0.195754 | 5_3:+9875978 | None:intergenic |
| GACAAAGCAATCAGGTTTAT+TGG | 0.268685 | 5_3:-9875808 | Msa0797650:CDS |
| TGTTGTTGAGGCTTGAATTC+TGG | 0.279950 | 5_3:+9875903 | None:intergenic |
| CACAACCTTTAACAGCAGTT+AGG | 0.395073 | 5_3:-9875983 | Msa0797650:CDS |
| CTTCACCATCTTCTCAGAGT+TGG | 0.407398 | 5_3:-9875773 | Msa0797650:CDS |
| CACCATCTTCTCAGAGTTGG+TGG | 0.431356 | 5_3:-9875770 | Msa0797650:CDS |
| ATTATGAGACTTAGGATAAG+AGG | 0.439722 | 5_3:+9876070 | None:intergenic |
| AATGAATTATTATGAGACTT+AGG | 0.448016 | 5_3:+9876062 | None:intergenic |
| ACAACCTTTAACAGCAGTTA+GGG | 0.458936 | 5_3:-9875982 | Msa0797650:CDS |
| ACCACTTTATCACTAACAAC+AGG | 0.505310 | 5_3:+9875855 | None:intergenic |
| AGTGGTGTGACAAAGCAATC+AGG | 0.507552 | 5_3:-9875816 | Msa0797650:CDS |
| CTAACTGCTGTTAAAGGTTG+TGG | 0.514313 | 5_3:+9875984 | None:intergenic |
| GACCACCAACTCTGAGAAGA+TGG | 0.520594 | 5_3:+9875768 | None:intergenic |
| ATTGTGGCTTTCTAACGGAA+TGG | 0.529161 | 5_3:+9876036 | None:intergenic |
| ACCTGTTGTTAGTGATAAAG+TGG | 0.557030 | 5_3:-9875856 | Msa0797650:CDS |
| AGACTTAGGATAAGAGGTGG+TGG | 0.557065 | 5_3:+9876076 | None:intergenic |
| GGTGGTGGAATAAGCTTCCA+TGG | 0.561828 | 5_3:+9876091 | None:intergenic |
| ACAAATTGTTGATGTTGTTG+AGG | 0.565750 | 5_3:+9875891 | None:intergenic |
| GAAAGCCACAATCAAAACCA+TGG | 0.621885 | 5_3:-9876025 | Msa0797650:CDS |
| GAGTTAGTTCAACAACTCAC+AGG | 0.631511 | 5_3:-9875930 | Msa0797650:CDS |
| GTGCGATATCAAGAGAGTAG+TGG | 0.659499 | 5_3:-9875834 | Msa0797650:CDS |
| ATGAGACTTAGGATAAGAGG+TGG | 0.667730 | 5_3:+9876073 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAATTATTATGAGACTT+AGG | + | chr5_3:9875785-9875804 | None:intergenic | 20.0% |
| ATTATGAGACTTAGGATAAG+AGG | + | chr5_3:9875777-9875796 | None:intergenic | 30.0% | |
| !! | TTTTGATTGTGGCTTTCTAA+CGG | + | chr5_3:9875816-9875835 | None:intergenic | 30.0% |
| !!! | TTTTTCCATGGTTTTGATTG+TGG | + | chr5_3:9875827-9875846 | None:intergenic | 30.0% |
| ACAAATTGTTGATGTTGTTG+AGG | + | chr5_3:9875956-9875975 | None:intergenic | 30.0% | |
| ACAACCTTTAACAGCAGTTA+GGG | - | chr5_3:9875862-9875881 | Msa0797650:CDS | 35.0% | |
| TAAACCCTAACTGCTGTTAA+AGG | + | chr5_3:9875869-9875888 | None:intergenic | 35.0% | |
| ACCTGTTGTTAGTGATAAAG+TGG | - | chr5_3:9875988-9876007 | Msa0797650:CDS | 35.0% | |
| ACCACTTTATCACTAACAAC+AGG | + | chr5_3:9875992-9876011 | None:intergenic | 35.0% | |
| GACAAAGCAATCAGGTTTAT+TGG | - | chr5_3:9876036-9876055 | Msa0797650:CDS | 35.0% | |
| ATGAGACTTAGGATAAGAGG+TGG | + | chr5_3:9875774-9875793 | None:intergenic | 40.0% | |
| ATTGTGGCTTTCTAACGGAA+TGG | + | chr5_3:9875811-9875830 | None:intergenic | 40.0% | |
| GAAAGCCACAATCAAAACCA+TGG | - | chr5_3:9875819-9875838 | Msa0797650:CDS | 40.0% | |
| ACCATGGAAAAAAGCTTCAG+TGG | - | chr5_3:9875835-9875854 | Msa0797650:CDS | 40.0% | |
| CTAACTGCTGTTAAAGGTTG+TGG | + | chr5_3:9875863-9875882 | None:intergenic | 40.0% | |
| CACAACCTTTAACAGCAGTT+AGG | - | chr5_3:9875861-9875880 | Msa0797650:CDS | 40.0% | |
| GAGTTAGTTCAACAACTCAC+AGG | - | chr5_3:9875914-9875933 | Msa0797650:CDS | 40.0% | |
| TGTTGTTGAGGCTTGAATTC+TGG | + | chr5_3:9875944-9875963 | None:intergenic | 40.0% | |
| AGACTTAGGATAAGAGGTGG+TGG | + | chr5_3:9875771-9875790 | None:intergenic | 45.0% | |
| !!! | GCCACTGAAGCTTTTTTCCA+TGG | + | chr5_3:9875839-9875858 | None:intergenic | 45.0% |
| GTGCGATATCAAGAGAGTAG+TGG | - | chr5_3:9876010-9876029 | Msa0797650:CDS | 45.0% | |
| AGTGGTGTGACAAAGCAATC+AGG | - | chr5_3:9876028-9876047 | Msa0797650:CDS | 45.0% | |
| CTTCACCATCTTCTCAGAGT+TGG | - | chr5_3:9876071-9876090 | Msa0797650:CDS | 45.0% | |
| CACCATCTTCTCAGAGTTGG+TGG | - | chr5_3:9876074-9876093 | Msa0797650:CDS | 50.0% | |
| ! | GACCACCAACTCTGAGAAGA+TGG | + | chr5_3:9876079-9876098 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 9875755 | 9876111 | 9875755 | ID=Msa0797650;Name=Msa0797650 |
| chr5_3 | mRNA | 9875755 | 9876111 | 9875755 | ID=Msa0797650-mRNA-1;Parent=Msa0797650;Name=Msa0797650-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|118 |
| chr5_3 | exon | 9875755 | 9876111 | 9875755 | ID=Msa0797650-mRNA-1:exon:4217;Parent=Msa0797650-mRNA-1 |
| chr5_3 | CDS | 9875755 | 9876111 | 9875755 | ID=Msa0797650-mRNA-1:cds;Parent=Msa0797650-mRNA-1 |
| Gene Sequence |
| Protein sequence |
