Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1389770 | XP_003592186.1 | 96.059 | 203 | 8 | 0 | 1 | 203 | 1 | 203 | 1.18e-144 | 410 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1389770 | G7IDY4 | 96.059 | 203 | 8 | 0 | 1 | 203 | 1 | 203 | 5.66e-145 | 410 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0225860 | Msa1389770 | 0.808712 | 2.706468e-50 | -8.615850e-47 |
| Msa1277220 | Msa1389770 | 0.800904 | 1.158014e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1389770 | MtrunA17_Chr1g0202491 | 96.059 | 203 | 8 | 0 | 1 | 203 | 1 | 203 | 1.09e-148 | 410 |
| Msa1389770 | MtrunA17_Chr6g0469901 | 51.250 | 240 | 78 | 5 | 1 | 201 | 1 | 240 | 3.32e-71 | 216 |
| Msa1389770 | MtrunA17_Chr8g0342361 | 41.379 | 203 | 91 | 6 | 1 | 194 | 1 | 184 | 1.07e-39 | 134 |
| Msa1389770 | MtrunA17_Chr8g0388471 | 30.457 | 197 | 122 | 6 | 1 | 191 | 1 | 188 | 8.20e-13 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1389770 | AT1G64700.1 | 40.816 | 196 | 80 | 6 | 1 | 173 | 1 | 183 | 6.58e-41 | 138 |
| Msa1389770 | AT3G61920.1 | 38.798 | 183 | 76 | 6 | 1 | 173 | 1 | 157 | 1.98e-27 | 103 |
Find 44 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAGAAGTCATGACCTCTTT+TGG | 0.226123 | tig0021300:+56112 | Msa1389770:CDS |
| AAAATTTCCATCAACTGTTC+TGG | 0.301136 | tig0021300:-56414 | None:intergenic |
| TGTCCAGTAGCAGCAGGTTT+TGG | 0.320414 | tig0021300:+56370 | Msa1389770:CDS |
| AACATGGGAAACTGCTTTGT+TGG | 0.338502 | tig0021300:+55970 | None:intergenic |
| ACATGGGAAACTGCTTTGTT+GGG | 0.369538 | tig0021300:+55971 | None:intergenic |
| GTGATGAAACTTACTGATAT+TGG | 0.369825 | tig0021300:-56063 | None:intergenic |
| TAATCTAAGGACATCCTATA+TGG | 0.379325 | tig0021300:-56297 | None:intergenic |
| TTGGTGGTGTTGATAATCTA+AGG | 0.399539 | tig0021300:-56310 | None:intergenic |
| TCTATATTGTTGTTGCTGAT+TGG | 0.412474 | tig0021300:-56189 | None:intergenic |
| AAACTGCTTTGTTGGGGCCA+TGG | 0.412625 | tig0021300:+55978 | Msa1389770:CDS |
| AACTCGTCGAATTGGCTTAG+TGG | 0.417992 | tig0021300:-56138 | None:intergenic |
| AACATCAAATGGTGGAATCA+TGG | 0.434935 | tig0021300:+56029 | Msa1389770:CDS |
| TTTCCAAAACCTGCTGCTAC+TGG | 0.436094 | tig0021300:-56373 | None:intergenic |
| AAGAGTTGATAGAAAGTGTA+AGG | 0.437401 | tig0021300:+56457 | Msa1389770:CDS |
| GGCTTAGTGGTTTCCAAAAG+AGG | 0.440233 | tig0021300:-56125 | None:intergenic |
| CCTCGTACCCTGCTCCATAT+AGG | 0.445768 | tig0021300:+56283 | Msa1389770:CDS |
| TCTTAAGAAACCTCATTCCT+TGG | 0.450979 | tig0021300:-56329 | None:intergenic |
| CTGTCTCTAACTCGTCGAAT+TGG | 0.467798 | tig0021300:-56146 | None:intergenic |
| GATTAGTCCAGAACAGTTGA+TGG | 0.472610 | tig0021300:+56407 | Msa1389770:CDS |
| ATCAAAATAATAACATCAAA+TGG | 0.473777 | tig0021300:+56018 | Msa1389770:CDS |
| TAAGAAACCTCATTCCTTGG+TGG | 0.480806 | tig0021300:-56326 | None:intergenic |
| AGCATGTCCAGGGAACTCTT+TGG | 0.482433 | tig0021300:-56085 | None:intergenic |
| AAGGACATCCTATATGGAGC+AGG | 0.492615 | tig0021300:-56291 | None:intergenic |
| AATCTTTGTCCAGTAGCAGC+AGG | 0.497074 | tig0021300:+56364 | Msa1389770:CDS |
| GGAGCAGGGTACGAGGTTGT+TGG | 0.507269 | tig0021300:-56276 | None:intergenic |
| CTGATCAGAAGCAATGTCTT+CGG | 0.510910 | tig0021300:-56514 | None:intergenic |
| CTTCTGAATATAGCATGTCC+AGG | 0.513789 | tig0021300:-56096 | None:intergenic |
| GGAGCCTTTCCAACACAAGC+TGG | 0.520882 | tig0021300:+56538 | Msa1389770:CDS |
| ATCAACACCACCAAGGAATG+AGG | 0.530419 | tig0021300:+56319 | Msa1389770:CDS |
| TTCATCACCAAAGAGTTCCC+TGG | 0.531410 | tig0021300:+56078 | Msa1389770:CDS |
| TGCTCCAGCTTGTGTTGGAA+AGG | 0.532611 | tig0021300:-56542 | None:intergenic |
| AAGACATTGCTTCTGATCAG+TGG | 0.581772 | tig0021300:+56517 | Msa1389770:CDS |
| CAATTCGACGAGTTAGAGAC+AGG | 0.584953 | tig0021300:+56147 | Msa1389770:CDS |
| CATGGGAAACTGCTTTGTTG+GGG | 0.602078 | tig0021300:+55972 | None:intergenic |
| TGAAATGCTCCAGCTTGTGT+TGG | 0.603544 | tig0021300:-56547 | None:intergenic |
| GTAAGGATTGTAGCAAAATG+TGG | 0.619505 | tig0021300:+56474 | Msa1389770:CDS |
| TTCTGAATATAGCATGTCCA+GGG | 0.624978 | tig0021300:-56095 | None:intergenic |
| CCTATATGGAGCAGGGTACG+AGG | 0.632716 | tig0021300:-56283 | None:intergenic |
| AAAATAATAACATCAAATGG+TGG | 0.636922 | tig0021300:+56021 | Msa1389770:CDS |
| AGGACATCCTATATGGAGCA+GGG | 0.647233 | tig0021300:-56290 | None:intergenic |
| TATGAACATGTTGTTAGACA+AGG | 0.651835 | tig0021300:+56231 | Msa1389770:CDS |
| AACAATATAGAAGCTTCATG+TGG | 0.666714 | tig0021300:+56201 | Msa1389770:CDS |
| TGTTGTATTAGAATCTGCCA+TGG | 0.666966 | tig0021300:-55995 | None:intergenic |
| TTAGATTATCAACACCACCA+AGG | 0.752136 | tig0021300:+56312 | Msa1389770:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCAAAATAATAACATCAAA+TGG | + | tig0021300:56018-56037 | Msa1389770:CDS | 15.0% |
| !! | AAAATAATAACATCAAATGG+TGG | + | tig0021300:56021-56040 | Msa1389770:CDS | 20.0% |
| GTGATGAAACTTACTGATAT+TGG | - | tig0021300:56066-56085 | None:intergenic | 30.0% | |
| ! | TCTATATTGTTGTTGCTGAT+TGG | - | tig0021300:56192-56211 | None:intergenic | 30.0% |
| AACAATATAGAAGCTTCATG+TGG | + | tig0021300:56201-56220 | Msa1389770:CDS | 30.0% | |
| TATGAACATGTTGTTAGACA+AGG | + | tig0021300:56231-56250 | Msa1389770:CDS | 30.0% | |
| TAATCTAAGGACATCCTATA+TGG | - | tig0021300:56300-56319 | None:intergenic | 30.0% | |
| AAAATTTCCATCAACTGTTC+TGG | - | tig0021300:56417-56436 | None:intergenic | 30.0% | |
| !! | AAGAGTTGATAGAAAGTGTA+AGG | + | tig0021300:56457-56476 | Msa1389770:CDS | 30.0% |
| TGTTGTATTAGAATCTGCCA+TGG | - | tig0021300:55998-56017 | None:intergenic | 35.0% | |
| ! | AACATCAAATGGTGGAATCA+TGG | + | tig0021300:56029-56048 | Msa1389770:CDS | 35.0% |
| TTCTGAATATAGCATGTCCA+GGG | - | tig0021300:56098-56117 | None:intergenic | 35.0% | |
| !! | TTGGTGGTGTTGATAATCTA+AGG | - | tig0021300:56313-56332 | None:intergenic | 35.0% |
| TTAGATTATCAACACCACCA+AGG | + | tig0021300:56312-56331 | Msa1389770:CDS | 35.0% | |
| TCTTAAGAAACCTCATTCCT+TGG | - | tig0021300:56332-56351 | None:intergenic | 35.0% | |
| !! | GATGGAAATTTTAGCACAAG+AGG | + | tig0021300:56425-56444 | Msa1389770:CDS | 35.0% |
| GTAAGGATTGTAGCAAAATG+TGG | + | tig0021300:56474-56493 | Msa1389770:CDS | 35.0% | |
| CTTCTGAATATAGCATGTCC+AGG | - | tig0021300:56099-56118 | None:intergenic | 40.0% | |
| ! | TCAGAAGTCATGACCTCTTT+TGG | + | tig0021300:56112-56131 | Msa1389770:CDS | 40.0% |
| TAAGAAACCTCATTCCTTGG+TGG | - | tig0021300:56329-56348 | None:intergenic | 40.0% | |
| !!! | CAGGTTTTGGAAAGTGAAGT+TGG | + | tig0021300:56383-56402 | Msa1389770:CDS | 40.0% |
| ! | GATTAGTCCAGAACAGTTGA+TGG | + | tig0021300:56407-56426 | Msa1389770:CDS | 40.0% |
| CTGATCAGAAGCAATGTCTT+CGG | - | tig0021300:56517-56536 | None:intergenic | 40.0% | |
| AAGACATTGCTTCTGATCAG+TGG | + | tig0021300:56517-56536 | Msa1389770:CDS | 40.0% | |
| TTCATCACCAAAGAGTTCCC+TGG | + | tig0021300:56078-56097 | Msa1389770:CDS | 45.0% | |
| GGCTTAGTGGTTTCCAAAAG+AGG | - | tig0021300:56128-56147 | None:intergenic | 45.0% | |
| ! | AACTCGTCGAATTGGCTTAG+TGG | - | tig0021300:56141-56160 | None:intergenic | 45.0% |
| CTGTCTCTAACTCGTCGAAT+TGG | - | tig0021300:56149-56168 | None:intergenic | 45.0% | |
| CAATTCGACGAGTTAGAGAC+AGG | + | tig0021300:56147-56166 | Msa1389770:CDS | 45.0% | |
| AGGACATCCTATATGGAGCA+GGG | - | tig0021300:56293-56312 | None:intergenic | 45.0% | |
| AAGGACATCCTATATGGAGC+AGG | - | tig0021300:56294-56313 | None:intergenic | 45.0% | |
| ATCAACACCACCAAGGAATG+AGG | + | tig0021300:56319-56338 | Msa1389770:CDS | 45.0% | |
| AATCTTTGTCCAGTAGCAGC+AGG | + | tig0021300:56364-56383 | Msa1389770:CDS | 45.0% | |
| TTTCCAAAACCTGCTGCTAC+TGG | - | tig0021300:56376-56395 | None:intergenic | 45.0% | |
| TGAAATGCTCCAGCTTGTGT+TGG | - | tig0021300:56550-56569 | None:intergenic | 45.0% | |
| ! | AAACTGCTTTGTTGGGGCCA+TGG | + | tig0021300:55978-55997 | Msa1389770:CDS | 50.0% |
| AGCATGTCCAGGGAACTCTT+TGG | - | tig0021300:56088-56107 | None:intergenic | 50.0% | |
| ! | TGTCCAGTAGCAGCAGGTTT+TGG | + | tig0021300:56370-56389 | Msa1389770:CDS | 50.0% |
| TGCTCCAGCTTGTGTTGGAA+AGG | - | tig0021300:56545-56564 | None:intergenic | 50.0% | |
| CCTATATGGAGCAGGGTACG+AGG | - | tig0021300:56286-56305 | None:intergenic | 55.0% | |
| CCTCGTACCCTGCTCCATAT+AGG | + | tig0021300:56283-56302 | Msa1389770:CDS | 55.0% | |
| GGAGCCTTTCCAACACAAGC+TGG | + | tig0021300:56538-56557 | Msa1389770:CDS | 55.0% | |
| GGAGCAGGGTACGAGGTTGT+TGG | - | tig0021300:56279-56298 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0021300 | gene | 55973 | 56584 | 55973 | ID=Msa1389770;Name=Msa1389770 |
| tig0021300 | mRNA | 55973 | 56584 | 55973 | ID=Msa1389770-mRNA-1;Parent=Msa1389770;Name=Msa1389770-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|203 |
| tig0021300 | exon | 55973 | 56584 | 55973 | ID=Msa1389770-mRNA-1:exon:11770;Parent=Msa1389770-mRNA-1 |
| tig0021300 | CDS | 55973 | 56584 | 55973 | ID=Msa1389770-mRNA-1:cds;Parent=Msa1389770-mRNA-1 |
| Gene Sequence |
| Protein sequence |
