AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1391610

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1391610 MtrunA17_Chr4g0071881 96.684 392 13 0 1 392 5 396 0.0 784
Msa1391610 MtrunA17_Chr2g0298571 69.610 385 111 3 7 391 5 383 0.0 546
Msa1391610 MtrunA17_Chr2g0298561 67.895 380 117 3 12 391 7 381 0.0 522
Msa1391610 MtrunA17_Chr4g0071891 64.881 336 111 5 58 391 7 337 1.09e-152 434
Msa1391610 MtrunA17_Chr4g0031691 24.925 333 219 11 51 363 79 400 7.19e-17 82.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1391610 AT4G25850.1 54.476 391 167 5 1 390 1 381 1.13e-146 421
Msa1391610 AT4G25850.2 54.476 391 167 5 1 390 103 483 1.06e-144 420
Msa1391610 AT5G57240.2 53.525 383 167 5 12 392 5 378 1.74e-144 415
Msa1391610 AT5G57240.5 53.525 383 167 5 12 392 5 378 1.74e-144 415
Msa1391610 AT5G57240.1 53.525 383 167 5 12 392 5 378 1.74e-144 415
Msa1391610 AT4G25860.1 52.196 387 176 4 5 390 4 382 2.25e-139 402
Msa1391610 AT4G25860.2 52.632 380 171 4 12 390 5 376 8.19e-139 401
Msa1391610 AT5G57240.4 50.245 408 167 6 12 392 5 403 1.83e-138 401
Msa1391610 AT5G57240.3 50.245 408 167 6 12 392 5 403 1.83e-138 401
Msa1391610 AT4G25850.3 57.455 275 107 5 1 273 1 267 7.35e-104 308
Msa1391610 AT5G59420.1 24.179 335 219 11 51 363 78 399 9.91e-14 72.8
Msa1391610 AT5G02100.3 23.724 333 223 11 51 363 51 372 1.38e-13 72.4
Msa1391610 AT5G02100.1 23.724 333 223 11 51 363 78 399 2.15e-13 72.0
Msa1391610 AT5G02100.2 29.586 169 110 5 51 217 78 239 7.61e-13 69.3

Find 126 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACTGAATCAGCTCTTATT+TGG 0.134175 tig0021440:-86336 Msa1391610:CDS
TAGCATTGAAGCTGTGATTT+TGG 0.181358 tig0021440:+87687 None:intergenic
TGGCTTTGTCAGAACAATTA+TGG 0.224454 tig0021440:+88096 None:intergenic
CTCCCAGTCATTGCTAATAA+TGG 0.230830 tig0021440:+86297 None:intergenic
ACCACACGTCCTACATCTTT+TGG 0.266880 tig0021440:-87555 Msa1391610:CDS
AGTACGTGGCTCTTTATATT+AGG 0.267147 tig0021440:-88838 Msa1391610:five_prime_UTR
AACTGAATCAGCTCTTATTT+GGG 0.293352 tig0021440:-86335 Msa1391610:CDS
GCTCCTTATAATCCCATTCT+TGG 0.296392 tig0021440:-87528 Msa1391610:CDS
GATGCAAAAGAAGTCATTTC+AGG 0.299497 tig0021440:-86518 Msa1391610:CDS
GGGAATAGTAAGTTGATTAA+AGG 0.299802 tig0021440:-86825 Msa1391610:CDS
AACTTCATGTTGAGTTTATT+CGG 0.304474 tig0021440:+85954 None:intergenic
CTGTTGTTGATCAGGGTGTT+TGG 0.313493 tig0021440:-86360 Msa1391610:intron
ATTAGAATTCTTCCAGTTCC+AGG 0.322110 tig0021440:-86945 Msa1391610:CDS
GGGTTGGGATTGTTAATATA+CGG 0.332141 tig0021440:-86914 Msa1391610:CDS
GAAACTTTGGAACAGCTTGC+TGG 0.333697 tig0021440:+87385 None:intergenic
AGCAGGTACCCATGTATTAA+TGG 0.333978 tig0021440:+86132 None:intergenic
GGCTTTGTCAGAACAATTAT+GGG 0.356535 tig0021440:+88097 None:intergenic
TTCCAGGAGTTAGTTGGGTT+GGG 0.357671 tig0021440:-86929 Msa1391610:CDS
TCTCCAAGAATGGGATTATA+AGG 0.360041 tig0021440:+87525 None:intergenic
AGGAAAACATTGAAATGATA+TGG 0.367856 tig0021440:-87410 Msa1391610:CDS
AGCAGATCTCACTAACTTAC+AGG 0.371004 tig0021440:-87972 Msa1391610:intron
TCCAGTTCCAGGAGTTAGTT+GGG 0.392005 tig0021440:-86934 Msa1391610:CDS
AAGCTGAAGTTCATGGCAAA+AGG 0.393826 tig0021440:-87025 Msa1391610:CDS
TTTCTGTCTGTAAAGTTAAG+AGG 0.393991 tig0021440:-88469 Msa1391610:five_prime_UTR
GTTCTAAAACTCAGAAATCA+TGG 0.418453 tig0021440:-87002 Msa1391610:CDS
GGAATAGTAAGTTGATTAAA+GGG 0.418791 tig0021440:-86824 Msa1391610:CDS
CGTGATGATGTGTCTAATTT+CGG 0.420816 tig0021440:+85909 None:intergenic
AGCATTGAAGCTGAAGTTCA+TGG 0.426779 tig0021440:-87032 Msa1391610:CDS
TTTGAGATACCGCGAAACTT+TGG 0.430109 tig0021440:+87372 None:intergenic
TTCCAGTTCCAGGAGTTAGT+TGG 0.435182 tig0021440:-86935 Msa1391610:CDS
ACCTCTGCATCCTTGACAAT+GGG 0.436791 tig0021440:+86485 None:intergenic
GTTCCAGGAGTTAGTTGGGT+TGG 0.438089 tig0021440:-86930 Msa1391610:CDS
GATTCAACTTCCAATGAGAA+TGG 0.439825 tig0021440:+88076 None:intergenic
CTTGTCATCTGTTGCGTGGA+GGG 0.441844 tig0021440:+87431 None:intergenic
GATGATTTGTTAAGCATAGT+TGG 0.444318 tig0021440:-88796 Msa1391610:five_prime_UTR
TATATGACATTGATGGTCAA+TGG 0.448483 tig0021440:-86770 Msa1391610:CDS
GCAGGTACCCATGTATTAAT+GGG 0.452463 tig0021440:+86133 None:intergenic
TCTGACAAAGCCATTCTCAT+TGG 0.453093 tig0021440:-88086 Msa1391610:CDS
ACCCAACTAACTCCTGGAAC+TGG 0.454137 tig0021440:+86933 None:intergenic
TAAGTTAGTGAGATCTGCTC+CGG 0.454449 tig0021440:+87976 None:intergenic
TCAGATTCAGAACCGAGTTA+TGG 0.462093 tig0021440:-88055 Msa1391610:CDS
GGCAGATACCGGAGGATTGG+TGG 0.462672 tig0021440:+87452 None:intergenic
AATGTACTATATGACATTGA+TGG 0.462851 tig0021440:-86777 Msa1391610:CDS
CAGACAGAAAGAGAACAATA+AGG 0.465422 tig0021440:+88482 None:intergenic
AAGCCATTATTAGCAATGAC+TGG 0.469518 tig0021440:-86300 Msa1391610:CDS
CTGTGATATGTGTTGCCTGT+TGG 0.480398 tig0021440:-88651 Msa1391610:five_prime_UTR
ATCCCAACCCAACTAACTCC+TGG 0.481594 tig0021440:+86927 None:intergenic
AATATACGGTGCAAAGAGAC+TGG 0.484332 tig0021440:-86900 Msa1391610:CDS
TGATATGTGTTGCCTGTTGG+AGG 0.486120 tig0021440:-88648 Msa1391610:five_prime_UTR
ATGGTGTGTCTCTCCAAGAA+TGG 0.490659 tig0021440:+87515 None:intergenic
ATATGACATTGATGGTCAAT+GGG 0.491161 tig0021440:-86769 Msa1391610:CDS
TTACTAAAGGCAACTACTGA+AGG 0.491965 tig0021440:-88130 Msa1391610:intron
ATTATATTAGTATGCGGTTG+CGG 0.497813 tig0021440:-88556 Msa1391610:five_prime_UTR
TCCAAGTCCAAGAAAAGAAT+AGG 0.500631 tig0021440:+86848 None:intergenic
GAGGGCAGATACCGGAGGAT+TGG 0.501864 tig0021440:+87449 None:intergenic
TGGTGTGTCTCTCCAAGAAT+GGG 0.506010 tig0021440:+87516 None:intergenic
ATGCAAAAGAAGTCATTTCA+GGG 0.510894 tig0021440:-86517 Msa1391610:CDS
ATTGTTCACCCATTAATACA+TGG 0.517678 tig0021440:-86141 Msa1391610:CDS
AGGAGTCCAACGTTCCCCTC+TGG 0.519515 tig0021440:+86210 None:intergenic
AGCTGAAGTTCATGGCAAAA+GGG 0.523135 tig0021440:-87024 Msa1391610:CDS
CAAGCTGTTCCAAAGTTTCG+CGG 0.524100 tig0021440:-87381 Msa1391610:intron
GTTGCGTGGAGGGCAGATAC+CGG 0.524211 tig0021440:+87441 None:intergenic
CAACAATGAGCAGAGTCCAC+TGG 0.527579 tig0021440:-87613 Msa1391610:CDS
AACAAGCGACGTATAAGATT+AGG 0.528065 tig0021440:+88022 None:intergenic
TTCTAAAACTCAGAAATCAT+GGG 0.532310 tig0021440:-87001 Msa1391610:CDS
GCAGGTTTCCACCAATCCTC+CGG 0.534023 tig0021440:-87460 Msa1391610:CDS
GACGTATAAGATTAGGTGCT+CGG 0.534128 tig0021440:+88029 None:intergenic
CCTTAACGTCCTACTCGAGC+AGG 0.539340 tig0021440:-87478 Msa1391610:CDS
CCTTGTCATCTGTTGCGTGG+AGG 0.541491 tig0021440:+87430 None:intergenic
TAGGACGTTAAGGTTACCCT+TGG 0.541780 tig0021440:+87488 None:intergenic
GGGGATATGATAGGTGCAGC+AGG 0.547965 tig0021440:+86115 None:intergenic
TAAGGTTACCCTTGGAAACA+TGG 0.549476 tig0021440:+87496 None:intergenic
ACATTGATGGTCAATGGGAT+AGG 0.553890 tig0021440:-86764 Msa1391610:intron
CTAAATCATATCCTATCGTA+TGG 0.559079 tig0021440:-88383 Msa1391610:intron
GTGTCTTACAGTAATGAAAG+TGG 0.561785 tig0021440:-86175 Msa1391610:CDS
TTATTGCTAATGAAATTGCT+TGG 0.565291 tig0021440:+86061 None:intergenic
AGAGACACACCATGTTTCCA+AGG 0.568528 tig0021440:-87505 Msa1391610:CDS
GGAGCAACTCCAAAAGATGT+AGG 0.569955 tig0021440:+87546 None:intergenic
TTCATTATTATATTAGTATG+CGG 0.578551 tig0021440:-88562 Msa1391610:five_prime_UTR
TTACAGTAATGAAAGTGGGT+TGG 0.581773 tig0021440:-86170 Msa1391610:CDS
CATATGAAATGAATTGTCCT+CGG 0.582471 tig0021440:-86974 Msa1391610:CDS
TACCTCTGCATCCTTGACAA+TGG 0.586448 tig0021440:+86484 None:intergenic
ATGAGCAGAGTCCACTGGAA+AGG 0.592131 tig0021440:-87608 Msa1391610:CDS
AAATCACAGCTTCAATGCTA+CGG 0.596960 tig0021440:-87684 Msa1391610:CDS
AAAGCAAAAGAAGCAAAGCA+AGG 0.598716 tig0021440:-86274 Msa1391610:CDS
AAAAGAATAGGATGATTCGT+AGG 0.600634 tig0021440:+86860 None:intergenic
TACTAAAGGCAACTACTGAA+GGG 0.600949 tig0021440:-88129 Msa1391610:intron
GGACGGACATACCATACGAT+AGG 0.604156 tig0021440:+88372 None:intergenic
TTGTTCACCCATTAATACAT+GGG 0.606408 tig0021440:-86140 Msa1391610:CDS
GGTTCACATCTGTGGTAGCG+TGG 0.606610 tig0021440:-87587 Msa1391610:CDS
GATATGTGTTGCCTGTTGGA+GGG 0.607543 tig0021440:-88647 Msa1391610:five_prime_UTR
ACTGGAAAGGTTCACATCTG+TGG 0.609418 tig0021440:-87595 Msa1391610:CDS
GAGACACCAGAGGGGAACGT+TGG 0.611769 tig0021440:-86216 Msa1391610:CDS
TTGAAAGAAAGAGACACCAG+AGG 0.616272 tig0021440:-86226 Msa1391610:CDS
TTTGGGAAATAAAATAGAGG+CGG 0.617018 tig0021440:+87705 None:intergenic
TCCCATTGTCAAGGATGCAG+AGG 0.618765 tig0021440:-86486 Msa1391610:intron
CCTCCACGCAACAGATGACA+AGG 0.623006 tig0021440:-87430 Msa1391610:CDS
AGCCATTATTAGCAATGACT+GGG 0.623574 tig0021440:-86299 Msa1391610:CDS
ACTGAATCAGCTCTTATTTG+GGG 0.624865 tig0021440:-86334 Msa1391610:CDS
AAAAGAAGCAAAGCAAGGCG+TGG 0.625738 tig0021440:-86269 Msa1391610:CDS
GCTCAAAGCTCCCATTGTCA+AGG 0.627873 tig0021440:-86495 Msa1391610:CDS
TGGTGGAAACCTGCTCGAGT+AGG 0.629646 tig0021440:+87469 None:intergenic
AAGTTAGTGAGATCTGCTCC+GGG 0.630886 tig0021440:+87977 None:intergenic
AGAAGCAAAGCAAGGCGTGG+AGG 0.633160 tig0021440:-86266 Msa1391610:CDS
TCCAAAAGATGTAGGACGTG+TGG 0.636254 tig0021440:+87554 None:intergenic
TGAAAGAAAGAGACACCAGA+GGG 0.638284 tig0021440:-86225 Msa1391610:CDS
ATATAGTACATTGAATGATG+AGG 0.640551 tig0021440:+86788 None:intergenic
CGTAGGATAACTCAGCCACA+AGG 0.646570 tig0021440:+86877 None:intergenic
TTTCCTTGTCATCTGTTGCG+TGG 0.647389 tig0021440:+87427 None:intergenic
TGGCCACCTGAGTTAAAATG+TGG 0.647497 tig0021440:-85997 Msa1391610:three_prime_UTR
AGAATTCTAATTGAGAGCCG+AGG 0.648326 tig0021440:+86957 None:intergenic
TCTAAAACTCAGAAATCATG+GGG 0.654639 tig0021440:-87000 Msa1391610:CDS
TTACAGGACTGTTAACGTGA+AGG 0.654814 tig0021440:-86573 Msa1391610:intron
ACGTGAAGGATACAAACAGT+GGG 0.656469 tig0021440:-86559 Msa1391610:CDS
TGTCTTACAGTAATGAAAGT+GGG 0.672570 tig0021440:-86174 Msa1391610:CDS
GCGTGGAGGGCAGATACCGG+AGG 0.675420 tig0021440:+87444 None:intergenic
GTGCAAAGAGACTGGCCTTG+TGG 0.675498 tig0021440:-86892 Msa1391610:CDS
TATCACAGAAGCTAAACTTG+TGG 0.677922 tig0021440:-86017 Msa1391610:three_prime_UTR
GAGACACACCATGTTTCCAA+GGG 0.680569 tig0021440:-87504 Msa1391610:CDS
GGTGCTCGGTAACCATAACT+CGG 0.684787 tig0021440:+88043 None:intergenic
AACGTGAAGGATACAAACAG+TGG 0.684966 tig0021440:-86560 Msa1391610:CDS
ACAGATGTGAACCTTTCCAG+TGG 0.686752 tig0021440:+87597 None:intergenic
GGTGTAGTTGTAGTAGTACG+TGG 0.695666 tig0021440:-88852 Msa1391610:five_prime_UTR
GGCCACCTGAGTTAAAATGT+GGG 0.742877 tig0021440:-85996 Msa1391610:three_prime_UTR
TTAACAAATCATCAGAAACG+TGG 0.750134 tig0021440:+88806 None:intergenic
GAAAGAAAGAGACACCAGAG+GGG 0.761071 tig0021440:-86224 Msa1391610:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTAATACTAGT+TGG - tig0021440:86006-86025 Msa1391610:three_prime_UTR 10.0%
!!! TTTTTTTTTTAATACTAGTT+GGG - tig0021440:86007-86026 Msa1391610:three_prime_UTR 10.0%
!! TTCATTATTATATTAGTATG+CGG - tig0021440:86189-86208 Msa1391610:CDS 15.0%
!! GCACAATATTATAAAATATA+CGG + tig0021440:86929-86948 None:intergenic 15.0%
!! TATTAGACTCAAATATATAT+TGG + tig0021440:87620-87639 None:intergenic 15.0%
!! AAATTTGATATATGTATTAC+AGG - tig0021440:88162-88181 Msa1391610:intron 15.0%
!! AAAAAAAAAGATGAGAAACA+GGG + tig0021440:86408-86427 None:intergenic 20.0%
!! AAAAAAAAAAGATGAGAAAC+AGG + tig0021440:86409-86428 None:intergenic 20.0%
!! ATGAAATTGACAATATAATG+AGG + tig0021440:87421-87440 None:intergenic 20.0%
!!! AGTAAATTTCAAGTTGTAAA+TGG - tig0021440:87675-87694 Msa1391610:CDS 20.0%
!!! AGAAAATGAAAGGTATAATA+TGG + tig0021440:88039-88058 None:intergenic 20.0%
!! TTCATTACAAAGAAAATGAA+AGG + tig0021440:88049-88068 None:intergenic 20.0%
!!! CATTTTCTTTGTAATGAAAT+CGG - tig0021440:88051-88070 Msa1391610:CDS 20.0%
!! AGTTCTTAAAAGAACAATAT+TGG - tig0021440:88308-88327 Msa1391610:intron 20.0%
!!! GTTTTGTTGTTTTAATTAAG+TGG - tig0021440:88336-88355 Msa1391610:intron 20.0%
!! TCAAAAAGAAAAAAAGTTAG+GGG + tig0021440:88659-88678 None:intergenic 20.0%
!! GTCAAAAAGAAAAAAAGTTA+GGG + tig0021440:88660-88679 None:intergenic 20.0%
!! TGTCAAAAAGAAAAAAAGTT+AGG + tig0021440:88661-88680 None:intergenic 20.0%
! ATGTATTGTTGATCCAAAAA+CGG - tig0021440:86156-86175 Msa1391610:CDS 25.0%
!! GAAAATAATTGATCCGTTTT+TGG + tig0021440:86172-86191 None:intergenic 25.0%
!!! CATCTTTTTTTTTTCTTCCT+TGG - tig0021440:86415-86434 Msa1391610:intron 25.0%
!! TACTTGTTTAATGCTCTTTT+TGG - tig0021440:86504-86523 Msa1391610:CDS 25.0%
!! ACTTGTTTAATGCTCTTTTT+GGG - tig0021440:86505-86524 Msa1391610:CDS 25.0%
! TGTAGAAACTAATTTAAGCT+GGG - tig0021440:86573-86592 Msa1391610:intron 25.0%
! TTCTACTCTTTAACCAATTA+CGG - tig0021440:86822-86841 Msa1391610:CDS 25.0%
!! CGATAGACTAGATAATTTTT+AGG + tig0021440:86889-86908 None:intergenic 25.0%
!!! GATTTTGGGAAATAAAATAG+AGG + tig0021440:87052-87071 None:intergenic 25.0%
! AGGAAAACATTGAAATGATA+TGG - tig0021440:87341-87360 Msa1391610:intron 25.0%
! GACTCAAATATATATTGGAT+AGG + tig0021440:87615-87634 None:intergenic 25.0%
!!! TTCTAAAACTCAGAAATCAT+GGG - tig0021440:87750-87769 Msa1391610:intron 25.0%
!!! GGAATAGTAAGTTGATTAAA+GGG - tig0021440:87927-87946 Msa1391610:intron 25.0%
! ATATAGTACATTGAATGATG+AGG + tig0021440:87966-87985 None:intergenic 25.0%
! AATGTACTATATGACATTGA+TGG - tig0021440:87974-87993 Msa1391610:CDS 25.0%
!!! AAGTGGCTTGTTAATTTTAT+TGG - tig0021440:88353-88372 Msa1391610:intron 25.0%
! TAAGACACCAAAAAATTCTT+AGG + tig0021440:88564-88583 None:intergenic 25.0%
! CAAAAAGAAAAAAAGTTAGG+GGG + tig0021440:88658-88677 None:intergenic 25.0%
! TTATTGCTAATGAAATTGCT+TGG + tig0021440:88693-88712 None:intergenic 25.0%
! AACTTCATGTTGAGTTTATT+CGG + tig0021440:88800-88819 None:intergenic 25.0%
TTAACAAATCATCAGAAACG+TGG + tig0021440:85948-85967 None:intergenic 30.0%
!! GATGATTTGTTAAGCATAGT+TGG - tig0021440:85955-85974 Msa1391610:three_prime_UTR 30.0%
ATTATATTAGTATGCGGTTG+CGG - tig0021440:86195-86214 Msa1391610:CDS 30.0%
! AATGACTTCTGACATTTTCT+AGG + tig0021440:86248-86267 None:intergenic 30.0%
TTTCTGTCTGTAAAGTTAAG+AGG - tig0021440:86282-86301 Msa1391610:CDS 30.0%
CTAAATCATATCCTATCGTA+TGG - tig0021440:86368-86387 Msa1391610:intron 30.0%
! AAGTACTGTACTACAGTATA+AGG + tig0021440:86489-86508 None:intergenic 30.0%
AGTTTATGCAAATTGAGTGT+TGG + tig0021440:86542-86561 None:intergenic 30.0%
! CAGCTTAAATTAGTTTCTAC+AGG + tig0021440:86574-86593 None:intergenic 30.0%
CTGTAGAAACTAATTTAAGC+TGG - tig0021440:86572-86591 Msa1391610:intron 30.0%
TAAAGAGTAGAAGTGAAAGA+GGG + tig0021440:86814-86833 None:intergenic 30.0%
TTAAAGAGTAGAAGTGAAAG+AGG + tig0021440:86815-86834 None:intergenic 30.0%
TATAATATTGTGCTCACACT+AGG - tig0021440:86936-86955 Msa1391610:CDS 30.0%
TTTGGGAAATAAAATAGAGG+CGG + tig0021440:87049-87068 None:intergenic 30.0%
TATAAAGTAGATATGCCTCA+AGG + tig0021440:87502-87521 None:intergenic 30.0%
ATGCTTGTACCTAAAGTAAA+GGG + tig0021440:87704-87723 None:intergenic 30.0%
AATGCTTGTACCTAAAGTAA+AGG + tig0021440:87705-87724 None:intergenic 30.0%
!! GTTCTAAAACTCAGAAATCA+TGG - tig0021440:87749-87768 Msa1391610:intron 30.0%
! TCTAAAACTCAGAAATCATG+GGG - tig0021440:87751-87770 Msa1391610:intron 30.0%
CATATGAAATGAATTGTCCT+CGG - tig0021440:87777-87796 Msa1391610:intron 30.0%
AAAAGAATAGGATGATTCGT+AGG + tig0021440:87894-87913 None:intergenic 30.0%
! GAATCATCCTATTCTTTTCT+TGG - tig0021440:87896-87915 Msa1391610:intron 30.0%
!! GGGAATAGTAAGTTGATTAA+AGG - tig0021440:87926-87945 Msa1391610:intron 30.0%
! TATATGACATTGATGGTCAA+TGG - tig0021440:87981-88000 Msa1391610:CDS 30.0%
! ATATGACATTGATGGTCAAT+GGG - tig0021440:87982-88001 Msa1391610:CDS 30.0%
ATGCAAAAGAAGTCATTTCA+GGG - tig0021440:88234-88253 Msa1391610:intron 30.0%
AAACTGAATCAGCTCTTATT+TGG - tig0021440:88415-88434 Msa1391610:five_prime_UTR 30.0%
! AACTGAATCAGCTCTTATTT+GGG - tig0021440:88416-88435 Msa1391610:five_prime_UTR 30.0%
!!! TTGGACTCCTAAGAATTTTT+TGG - tig0021440:88554-88573 Msa1391610:five_prime_UTR 30.0%
TGTCTTACAGTAATGAAAGT+GGG - tig0021440:88577-88596 Msa1391610:five_prime_UTR 30.0%
ATTGTTCACCCATTAATACA+TGG - tig0021440:88610-88629 Msa1391610:five_prime_UTR 30.0%
TTGTTCACCCATTAATACAT+GGG - tig0021440:88611-88630 Msa1391610:five_prime_UTR 30.0%
AGTACGTGGCTCTTTATATT+AGG - tig0021440:85913-85932 Msa1391610:three_prime_UTR 35.0%
CAGACAGAAAGAGAACAATA+AGG + tig0021440:86272-86291 None:intergenic 35.0%
! AGAAGCATAAAGAACGGTAT+TGG + tig0021440:86443-86462 None:intergenic 35.0%
! ACCGTTCTTTATGCTTCTTT+TGG - tig0021440:86445-86464 Msa1391610:intron 35.0%
! CCCAAAAGAAGCATAAAGAA+CGG + tig0021440:86449-86468 None:intergenic 35.0%
! CCGTTCTTTATGCTTCTTTT+GGG - tig0021440:86446-86465 Msa1391610:intron 35.0%
TTACTAAAGGCAACTACTGA+AGG - tig0021440:86621-86640 Msa1391610:intron 35.0%
TACTAAAGGCAACTACTGAA+GGG - tig0021440:86622-86641 Msa1391610:intron 35.0%
! GGCTTTGTCAGAACAATTAT+GGG + tig0021440:86657-86676 None:intergenic 35.0%
!! TGGCTTTGTCAGAACAATTA+TGG + tig0021440:86658-86677 None:intergenic 35.0%
GATTCAACTTCCAATGAGAA+TGG + tig0021440:86678-86697 None:intergenic 35.0%
AACAAGCGACGTATAAGATT+AGG + tig0021440:86732-86751 None:intergenic 35.0%
TTACTTAAAAATGTGCGACC+CGG - tig0021440:86756-86775 Msa1391610:intron 35.0%
TTTAGGCATAAAGCACAGAT+TGG + tig0021440:86872-86891 None:intergenic 35.0%
TGGAAATTAACAAGTGACCT+TGG - tig0021440:87003-87022 Msa1391610:CDS 35.0%
!! AGCATTGAAGCTGTGATTTT+GGG + tig0021440:87066-87085 None:intergenic 35.0%
!! TAGCATTGAAGCTGTGATTT+TGG + tig0021440:87067-87086 None:intergenic 35.0%
AAATCACAGCTTCAATGCTA+CGG - tig0021440:87067-87086 Msa1391610:intron 35.0%
TCTCCAAGAATGGGATTATA+AGG + tig0021440:87229-87248 None:intergenic 35.0%
GTCAATTTCATATACGCTGA+CGG - tig0021440:87430-87449 Msa1391610:CDS 35.0%
CATTAAAAACATTCGCCTTG+AGG - tig0021440:87484-87503 Msa1391610:CDS 35.0%
AAATGGTGTCCCTTTACTTT+AGG - tig0021440:87692-87711 Msa1391610:CDS 35.0%
ATTAGAATTCTTCCAGTTCC+AGG - tig0021440:87806-87825 Msa1391610:intron 35.0%
GGGTTGGGATTGTTAATATA+CGG - tig0021440:87837-87856 Msa1391610:intron 35.0%
! TCCTATTCTTTTCTTGGACT+TGG - tig0021440:87902-87921 Msa1391610:intron 35.0%
TCCAAGTCCAAGAAAAGAAT+AGG + tig0021440:87906-87925 None:intergenic 35.0%
! TATTCTTTTCTTGGACTTGG+AGG - tig0021440:87905-87924 Msa1391610:intron 35.0%
! ATTCTTTTCTTGGACTTGGA+GGG - tig0021440:87906-87925 Msa1391610:intron 35.0%
! GAAAGGTATAATATGGTGTG+AGG + tig0021440:88032-88051 None:intergenic 35.0%
ACTAGCTAATCATTCACTAG+CGG - tig0021440:88094-88113 Msa1391610:CDS 35.0%
GATGCAAAAGAAGTCATTTC+AGG - tig0021440:88233-88252 Msa1391610:intron 35.0%
TGAGATTCTGTTGTTGATCA+GGG - tig0021440:88384-88403 Msa1391610:exon 35.0%
! ACTGAATCAGCTCTTATTTG+GGG - tig0021440:88417-88436 Msa1391610:five_prime_UTR 35.0%
AAGCCATTATTAGCAATGAC+TGG - tig0021440:88451-88470 Msa1391610:five_prime_UTR 35.0%
AGCCATTATTAGCAATGACT+GGG - tig0021440:88452-88471 Msa1391610:five_prime_UTR 35.0%
AAAGCAAAAGAAGCAAAGCA+AGG - tig0021440:88477-88496 Msa1391610:five_prime_UTR 35.0%
GTGTCTTACAGTAATGAAAG+TGG - tig0021440:88576-88595 Msa1391610:five_prime_UTR 35.0%
TTACAGTAATGAAAGTGGGT+TGG - tig0021440:88581-88600 Msa1391610:five_prime_UTR 35.0%
AAAAGTTAGGGGGATATGAT+AGG + tig0021440:88648-88667 None:intergenic 35.0%
TATCACAGAAGCTAAACTTG+TGG - tig0021440:88734-88753 Msa1391610:five_prime_UTR 35.0%
! CGTGATGATGTGTCTAATTT+CGG + tig0021440:88845-88864 None:intergenic 35.0%
!! ATAGTTGGTGTTTTGCTTGC+AGG - tig0021440:85970-85989 Msa1391610:three_prime_UTR 40.0%
!! TAGTTGGTGTTTTGCTTGCA+GGG - tig0021440:85971-85990 Msa1391610:three_prime_UTR 40.0%
AAAAGATGAGAAACAGGGAG+AGG + tig0021440:86403-86422 None:intergenic 40.0%
! AAAGAACGGTATTGGATCCA+AGG + tig0021440:86435-86454 None:intergenic 40.0%
! TTAAGCTGGGTGTTACATTG+TGG - tig0021440:86586-86605 Msa1391610:intron 40.0%
GCGGGCTTAACTATTACTAA+AGG - tig0021440:86608-86627 Msa1391610:intron 40.0%
TCTGACAAAGCCATTCTCAT+TGG - tig0021440:86665-86684 Msa1391610:intron 40.0%
TCAGATTCAGAACCGAGTTA+TGG - tig0021440:86696-86715 Msa1391610:intron 40.0%
GACGTATAAGATTAGGTGCT+CGG + tig0021440:86725-86744 None:intergenic 40.0%
TAAGTTAGTGAGATCTGCTC+CGG + tig0021440:86778-86797 None:intergenic 40.0%
AGCAGATCTCACTAACTTAC+AGG - tig0021440:86779-86798 Msa1391610:CDS 40.0%
GAGTTTCAACCAAATCACAG+TGG + tig0021440:86850-86869 None:intergenic 40.0%
TATTGTGCTCACACTAGGAT+AGG - tig0021440:86941-86960 Msa1391610:CDS 40.0%
CAATGCGTGTATTAGACAGA+CGG + tig0021440:86985-87004 None:intergenic 40.0%
GCTCCTTATAATCCCATTCT+TGG - tig0021440:87223-87242 Msa1391610:intron 40.0%
TAAGGTTACCCTTGGAAACA+TGG + tig0021440:87258-87277 None:intergenic 40.0%
! TTTGAGATACCGCGAAACTT+TGG + tig0021440:87382-87401 None:intergenic 40.0%
! AGCATTGAAGCTGAAGTTCA+TGG - tig0021440:87719-87738 Msa1391610:intron 40.0%
AAGCTGAAGTTCATGGCAAA+AGG - tig0021440:87726-87745 Msa1391610:intron 40.0%
AGCTGAAGTTCATGGCAAAA+GGG - tig0021440:87727-87746 Msa1391610:intron 40.0%
AGAATTCTAATTGAGAGCCG+AGG + tig0021440:87797-87816 None:intergenic 40.0%
AATATACGGTGCAAAGAGAC+TGG - tig0021440:87851-87870 Msa1391610:intron 40.0%
! ACATTGATGGTCAATGGGAT+AGG - tig0021440:87987-88006 Msa1391610:CDS 40.0%
AATATGGTGTGAGGTGATGA+GGG + tig0021440:88023-88042 None:intergenic 40.0%
TAATATGGTGTGAGGTGATG+AGG + tig0021440:88024-88043 None:intergenic 40.0%
TTACAGGACTGTTAACGTGA+AGG - tig0021440:88178-88197 Msa1391610:intron 40.0%
AACGTGAAGGATACAAACAG+TGG - tig0021440:88191-88210 Msa1391610:intron 40.0%
ACGTGAAGGATACAAACAGT+GGG - tig0021440:88192-88211 Msa1391610:intron 40.0%
CTGAGATTCTGTTGTTGATC+AGG - tig0021440:88383-88402 Msa1391610:intron 40.0%
CTCCCAGTCATTGCTAATAA+TGG + tig0021440:88457-88476 None:intergenic 40.0%
TTGAAAGAAAGAGACACCAG+AGG - tig0021440:88525-88544 Msa1391610:five_prime_UTR 40.0%
TGAAAGAAAGAGACACCAGA+GGG - tig0021440:88526-88545 Msa1391610:five_prime_UTR 40.0%
GCAGGTACCCATGTATTAAT+GGG + tig0021440:88621-88640 None:intergenic 40.0%
AGCAGGTACCCATGTATTAA+TGG + tig0021440:88622-88641 None:intergenic 40.0%
!!! GATTGCCCACATTTTAACTC+AGG + tig0021440:88763-88782 None:intergenic 40.0%
GGTGTAGTTGTAGTAGTACG+TGG - tig0021440:85899-85918 Msa1391610:three_prime_UTR 45.0%
CTGTGATATGTGTTGCCTGT+TGG - tig0021440:86100-86119 Msa1391610:exon 45.0%
TGATATGTGTTGCCTGTTGG+AGG - tig0021440:86103-86122 Msa1391610:exon 45.0%
GATATGTGTTGCCTGTTGGA+GGG - tig0021440:86104-86123 Msa1391610:exon 45.0%
! GTTTTACTCTACCCTCCAAC+AGG + tig0021440:86118-86137 None:intergenic 45.0%
AAGTTAGTGAGATCTGCTCC+GGG + tig0021440:86777-86796 None:intergenic 45.0%
AATCACAGTGGCACCGTAAT+TGG + tig0021440:86838-86857 None:intergenic 45.0%
!! ATTACGGTGCCACTGTGATT+TGG - tig0021440:86838-86857 Msa1391610:CDS 45.0%
CGTCTGTCTAATACACGCAT+TGG - tig0021440:86983-87002 Msa1391610:CDS 45.0%
ACAGATGTGAACCTTTCCAG+TGG + tig0021440:87157-87176 None:intergenic 45.0%
ACTGGAAAGGTTCACATCTG+TGG - tig0021440:87156-87175 Msa1391610:intron 45.0%
! ACCACACGTCCTACATCTTT+TGG - tig0021440:87196-87215 Msa1391610:intron 45.0%
TCCAAAAGATGTAGGACGTG+TGG + tig0021440:87200-87219 None:intergenic 45.0%
GGAGCAACTCCAAAAGATGT+AGG + tig0021440:87208-87227 None:intergenic 45.0%
TGGTGTGTCTCTCCAAGAAT+GGG + tig0021440:87238-87257 None:intergenic 45.0%
ATGGTGTGTCTCTCCAAGAA+TGG + tig0021440:87239-87258 None:intergenic 45.0%
AGAGACACACCATGTTTCCA+AGG - tig0021440:87246-87265 Msa1391610:intron 45.0%
GAGACACACCATGTTTCCAA+GGG - tig0021440:87247-87266 Msa1391610:intron 45.0%
TAGGACGTTAAGGTTACCCT+TGG + tig0021440:87266-87285 None:intergenic 45.0%
TTTCCTTGTCATCTGTTGCG+TGG + tig0021440:87327-87346 None:intergenic 45.0%
!! GAAACTTTGGAACAGCTTGC+TGG + tig0021440:87369-87388 None:intergenic 45.0%
CAAGCTGTTCCAAAGTTTCG+CGG - tig0021440:87370-87389 Msa1391610:intron 45.0%
TTCCAGTTCCAGGAGTTAGT+TGG - tig0021440:87816-87835 Msa1391610:intron 45.0%
TCCAGTTCCAGGAGTTAGTT+GGG - tig0021440:87817-87836 Msa1391610:intron 45.0%
TTCCAGGAGTTAGTTGGGTT+GGG - tig0021440:87822-87841 Msa1391610:intron 45.0%
ACCTCTGCATCCTTGACAAT+GGG + tig0021440:88269-88288 None:intergenic 45.0%
TACCTCTGCATCCTTGACAA+TGG + tig0021440:88270-88289 None:intergenic 45.0%
GGATGCAGAGGTAACTAACA+AGG - tig0021440:88277-88296 Msa1391610:intron 45.0%
! CTGTTGTTGATCAGGGTGTT+TGG - tig0021440:88391-88410 Msa1391610:five_prime_UTR 45.0%
AAAAGAAGCAAAGCAAGGCG+TGG - tig0021440:88482-88501 Msa1391610:five_prime_UTR 45.0%
GAAAGAAAGAGACACCAGAG+GGG - tig0021440:88527-88546 Msa1391610:five_prime_UTR 45.0%
TGGCCACCTGAGTTAAAATG+TGG - tig0021440:88754-88773 Msa1391610:five_prime_UTR 45.0%
GGCCACCTGAGTTAAAATGT+GGG - tig0021440:88755-88774 Msa1391610:five_prime_UTR 45.0%
!!! TGCCCACATTTTAACTCAGG+TGG + tig0021440:88760-88779 None:intergenic 45.0%
GGACGGACATACCATACGAT+AGG + tig0021440:86382-86401 None:intergenic 50.0%
GATGAGAAACAGGGAGAGGA+CGG + tig0021440:86399-86418 None:intergenic 50.0%
! AGCTGGGTGTTACATTGTGG+CGG - tig0021440:86589-86608 Msa1391610:intron 50.0%
GGTGCTCGGTAACCATAACT+CGG + tig0021440:86711-86730 None:intergenic 50.0%
CTGCACACGAATACAAGCCA+AGG + tig0021440:87023-87042 None:intergenic 50.0%
CAACAATGAGCAGAGTCCAC+TGG - tig0021440:87138-87157 Msa1391610:intron 50.0%
ATGAGCAGAGTCCACTGGAA+AGG - tig0021440:87143-87162 Msa1391610:intron 50.0%
CTTGTCATCTGTTGCGTGGA+GGG + tig0021440:87323-87342 None:intergenic 50.0%
ACCCAACTAACTCCTGGAAC+TGG + tig0021440:87821-87840 None:intergenic 50.0%
GTTCCAGGAGTTAGTTGGGT+TGG - tig0021440:87821-87840 Msa1391610:intron 50.0%
ATCCCAACCCAACTAACTCC+TGG + tig0021440:87827-87846 None:intergenic 50.0%
CGTAGGATAACTCAGCCACA+AGG + tig0021440:87877-87896 None:intergenic 50.0%
GCTCAAAGCTCCCATTGTCA+AGG - tig0021440:88256-88275 Msa1391610:intron 50.0%
TCCCATTGTCAAGGATGCAG+AGG - tig0021440:88265-88284 Msa1391610:intron 50.0%
! GCTGGGTGTTACATTGTGGC+GGG - tig0021440:86590-86609 Msa1391610:intron 55.0%
! GGTTCACATCTGTGGTAGCG+TGG - tig0021440:87164-87183 Msa1391610:intron 55.0%
CCTGCTCGAGTAGGACGTTA+AGG + tig0021440:87276-87295 None:intergenic 55.0%
CCTTAACGTCCTACTCGAGC+AGG - tig0021440:87273-87292 Msa1391610:intron 55.0%
TGGTGGAAACCTGCTCGAGT+AGG + tig0021440:87285-87304 None:intergenic 55.0%
GCAGGTTTCCACCAATCCTC+CGG - tig0021440:87291-87310 Msa1391610:intron 55.0%
CCTTGTCATCTGTTGCGTGG+AGG + tig0021440:87324-87343 None:intergenic 55.0%
CCTCCACGCAACAGATGACA+AGG - tig0021440:87321-87340 Msa1391610:intron 55.0%
!! GTGCAAAGAGACTGGCCTTG+TGG - tig0021440:87859-87878 Msa1391610:intron 55.0%
AGAAGCAAAGCAAGGCGTGG+AGG - tig0021440:88485-88504 Msa1391610:five_prime_UTR 55.0%
GGGGATATGATAGGTGCAGC+AGG + tig0021440:88639-88658 None:intergenic 55.0%
GGCAGATACCGGAGGATTGG+TGG + tig0021440:87302-87321 None:intergenic 60.0%
GAGGGCAGATACCGGAGGAT+TGG + tig0021440:87305-87324 None:intergenic 60.0%
GTTGCGTGGAGGGCAGATAC+CGG + tig0021440:87313-87332 None:intergenic 60.0%
GAGACACCAGAGGGGAACGT+TGG - tig0021440:88535-88554 Msa1391610:five_prime_UTR 60.0%
AGGAGTCCAACGTTCCCCTC+TGG + tig0021440:88544-88563 None:intergenic 60.0%
GCGTGGAGGGCAGATACCGG+AGG + tig0021440:87310-87329 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
tig0021440 gene 85895 88878 85895 ID=Msa1391610;Name=Msa1391610
tig0021440 mRNA 85895 88878 85895 ID=Msa1391610-mRNA-1;Parent=Msa1391610;Name=Msa1391610-mRNA-1;_AED=0.10;_eAED=0.10;_QI=492|1|1|1|0.6|0.66|6|216|392
tig0021440 exon 85895 86368 85895 ID=Msa1391610-mRNA-1:exon:12769;Parent=Msa1391610-mRNA-1
tig0021440 exon 86487 86589 86487 ID=Msa1391610-mRNA-1:exon:12768;Parent=Msa1391610-mRNA-1
tig0021440 exon 86765 87059 86765 ID=Msa1391610-mRNA-1:exon:12767;Parent=Msa1391610-mRNA-1
tig0021440 exon 87382 87730 87382 ID=Msa1391610-mRNA-1:exon:12766;Parent=Msa1391610-mRNA-1
tig0021440 exon 87973 88143 87973 ID=Msa1391610-mRNA-1:exon:12765;Parent=Msa1391610-mRNA-1
tig0021440 exon 88384 88878 88384 ID=Msa1391610-mRNA-1:exon:12764;Parent=Msa1391610-mRNA-1
tig0021440 five_prime_UTR 88387 88878 88387 ID=Msa1391610-mRNA-1:five_prime_utr;Parent=Msa1391610-mRNA-1
tig0021440 CDS 88384 88386 88384 ID=Msa1391610-mRNA-1:cds;Parent=Msa1391610-mRNA-1
tig0021440 CDS 87973 88143 87973 ID=Msa1391610-mRNA-1:cds;Parent=Msa1391610-mRNA-1
tig0021440 CDS 87382 87730 87382 ID=Msa1391610-mRNA-1:cds;Parent=Msa1391610-mRNA-1
tig0021440 CDS 86765 87059 86765 ID=Msa1391610-mRNA-1:cds;Parent=Msa1391610-mRNA-1
tig0021440 CDS 86487 86589 86487 ID=Msa1391610-mRNA-1:cds;Parent=Msa1391610-mRNA-1
tig0021440 CDS 86111 86368 86111 ID=Msa1391610-mRNA-1:cds;Parent=Msa1391610-mRNA-1
tig0021440 three_prime_UTR 85895 86110 85895 ID=Msa1391610-mRNA-1:three_prime_utr;Parent=Msa1391610-mRNA-1
Gene Sequence

>Msa1391610

ATGGCAACTACTGAAGGGAAGAAGCAGCCCATAATTGTTCTGACAAAGCCATTCTCATTGGAAGTTGAATCAGATTCAGAACCGAGTTATGGTTACCGAGCACCTAATCTTATACGTCGCTTGTTAAGTTTACTTAAAAATGTGCGACCCGGAGCAGATCTCACTAACTTACAGCTTCCGCCTCTATTTTATTTCCCAAAATCACAGCTTCAATGCTACGGTGAATCAGTATACAGCACAACTTCAAATTCAAACTTGCTAAACAAATGCAACAATGAGCAGAGTCCACTGGAAAGGTTCACATCTGTGGTAGCGTGGAGCATATCTACCACACGTCCTACATCTTTTGGAGTTGCTCCTTATAATCCCATTCTTGGAGAGACACACCATGTTTCCAAGGGTAACCTTAACGTCCTACTCGAGCAGGTTTCCACCAATCCTCCGGTATCTGCCCTCCACGCAACAGATGACAAGGAAAACATTGAAATGATATGGTGCCAGCAAGCTGTTCCAAAGTTTCGCGGTACAAGCATTGAAGCTGAAGTTCATGGCAAAAGGGTTCTAAAACTCAGAAATCATGGGGAAACATATGAAATGAATTGTCCTCGGCTCTCAATTAGAATTCTTCCAGTTCCAGGAGTTAGTTGGGTTGGGATTGTTAATATACGGTGCAAAGAGACTGGCCTTGTGGCTGAGTTATCCTACGAATCATCCTATTCTTTTCTTGGACTTGGAGGGAATAGTAAGTTGATTAAAGGGAAGATCGTTGATTCCTCATCATTCAATGTACTATATGACATTGATGGTCAATGGGATAGGACTGTTAACGTGAAGGATACAAACAGTGGGAAAGTCAAAGTGATATACGATGCAAAAGAAGTCATTTCAGGGCTCAAAGCTCCCATTGTCAAGGATGCAGAGGGTGTTTGGCAAACTGAATCAGCTCTTATTTGGGGTGAGTTGACTCAAGCCATTATTAGCAATGACTGGGAGAAAGCAAAAGAAGCAAAGCAAGGCGTGGAGGAAAGACAAAGTAAGATGTTGAAAGAAAGAGACACCAGAGGGGAACGTTGGACTCCTAAGAATTTTTTGGTGTCTTACAGTAATGAAAGTGGGTTGGACTGTGATTGTTCACCCATTAATACATGGGTACCTGCTGCACCTATCATATCCCCCTAA
Protein sequence

>Msa1391610

MATTEGKKQPIIVLTKPFSLEVESDSEPSYGYRAPNLIRRLLSLLKNVRPGADLTNLQLPPLFYFPKSQLQCYGESVYSTTSNSNLLNKCNNEQSPLERFTSVVAWSISTTRPTSFGVAPYNPILGETHHVSKGNLNVLLEQVSTNPPVSALHATDDKENIEMIWCQQAVPKFRGTSIEAEVHGKRVLKLRNHGETYEMNCPRLSIRILPVPGVSWVGIVNIRCKETGLVAELSYESSYSFLGLGGNSKLIKGKIVDSSSFNVLYDIDGQWDRTVNVKDTNSGKVKVIYDAKEVISGLKAPIVKDAEGVWQTESALIWGELTQAIISNDWEKAKEAKQGVEERQSKMLKERDTRGERWTPKNFLVSYSNESGLDCDCSPINTWVPAAPIISP*